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2012

Biology

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Full-Text Articles in Life Sciences

Comparing Biology Grades Based On Instructional Delivery And Instructor At A Community College: Face-To-Face Course Versus Online Course., Amanda Rosenzweig Dec 2012

Comparing Biology Grades Based On Instructional Delivery And Instructor At A Community College: Face-To-Face Course Versus Online Course., Amanda Rosenzweig

University of New Orleans Theses and Dissertations

Through distance learning, the community college system has been able to serve more students by providing educational opportunities to students who would otherwise be unable to attend college. The community college of focus in the study increased its online enrollments and online course offerings due to the growth of overall enrollment. The need and purpose of the study is to address if there is a difference in students’ grades between face-to-face and online biology related courses and if there are differences in grades between face-to-face and online biology courses taught by different instructors and the same instructor. The study also …


Secondary Structure, A Missing Component Of Sequence- Based Minimotif Definitions, David P. Sargeant, Michael R. Gryk, Mark W. Maciejewsk, Vishal Thapar, Vamsi Kundeti, Sanguthevar Rajasekaran, Pedro Romero, Keith Dunker, Shun-Cheng Li, Tomonori Kaneko, Martin Schiller Dec 2012

Secondary Structure, A Missing Component Of Sequence- Based Minimotif Definitions, David P. Sargeant, Michael R. Gryk, Mark W. Maciejewsk, Vishal Thapar, Vamsi Kundeti, Sanguthevar Rajasekaran, Pedro Romero, Keith Dunker, Shun-Cheng Li, Tomonori Kaneko, Martin Schiller

Life Sciences Faculty Research

Minimotifs are short contiguous segments of proteins that have a known biological function. The hundreds of thousands of minimotifs discovered thus far are an important part of the theoretical understanding of the specificity of protein-protein interactions, posttranslational modifications, and signal transduction that occur in cells. However, a longstanding problem is that the different abstractions of the sequence definitions do not accurately capture the specificity, despite decades of effort by many labs. We present evidence that structure is an essential component of minimotif specificity, yet is not used in minimotif definitions. Our analysis of several known minimotifs as case studies, analysis …


Simple, Compact Source For Low-Temperature Air Plasmas, D. Sheehan, J. Lawson, M. Sosa, Richard Long Oct 2012

Simple, Compact Source For Low-Temperature Air Plasmas, D. Sheehan, J. Lawson, M. Sosa, Richard Long

Richard A. Long

A simple, compact source of low-temperature, spatially and temporally uniform air plasma using a Telsa induction coil driver is described. The low-power ionization discharge plasma is localized (2 cm X 0.5 cm X 0.1 cm) and essentially free of arc channels. A Teflon coated rolling cylindrical electrode and dielectric coated ground plate are essential to the source’s operation and allow flat test samples to be readily exposed to the plasma. The plasma is a copious source of ozone and nitrogen oxides. Its effects on various microbes are discussed.


Individual Cell Growth Rates Of Marine Bacteria, Measured By Bromodeoxyuridine Incorporation, Koji Hamasaki, Richard A. Long, Farooq Azam Oct 2012

Individual Cell Growth Rates Of Marine Bacteria, Measured By Bromodeoxyuridine Incorporation, Koji Hamasaki, Richard A. Long, Farooq Azam

Richard A. Long

We tested the application of 5-bromo-2’-deoxyuridine (BrdU), a thymidine analogue that becomes incorporated into DNA during growth, to measure growth rates of individual marine bacteria cells. Immunocytochemical detection of BrdU incorporation into bacterial DNA has the potential for single-cell-based growth measurement. Optimized procedure for immunocytochemistry was applicable to 14 marine heterotrophic bacterial isolates belonging to g-proteobacteria, α-proteobacteria, Cytophaga-Flavobacterium-Bacteroides (CFB) group and Gram-positive bacteria. The relationship between cell-specific fluorescence intensity and specific growth rate was linearly correlated among CFB group isolates, which indicated a potential of the method for quantitative measurement. Analysis of the detection limit indicated …


Coupling Between Bacterioplankton Species Composition, Population Dynamics, And Organic Matter Degradation, Jarone Pinhassi, Farooq Azam, Johanna Hemphälä, Richard A. Long, Josefina Martinez, Ulla Li Zweifel, Åke Hagström Oct 2012

Coupling Between Bacterioplankton Species Composition, Population Dynamics, And Organic Matter Degradation, Jarone Pinhassi, Farooq Azam, Johanna Hemphälä, Richard A. Long, Josefina Martinez, Ulla Li Zweifel, Åke Hagström

Richard A. Long

To study the effect of substrate addition on short term bacterial population dynamics and species composition in seawater mesocosms were maintained with water collected off Scripps Pier (La Jolla, California USA) Protein enrichment (BSA) triggered a dynamic response from the microbial food web, whereas enrichment with starch had no effect. In the protein enriched mesocosm the number of both nucleoid-containing cells and metabolically active cells increased by 3.0 X 105 cells ml-1 from Day 1 to Day 4 In the same time period the density of a set of 31 phylogenetically different bacteria (α- and g-Proteobactena as well as Flexibacter-Cytophaga-Bacteroides) …


Abundant Protein-Containing Particles In The Sea, Richard A. Long, Farooq Azam Oct 2012

Abundant Protein-Containing Particles In The Sea, Richard A. Long, Farooq Azam

Richard A. Long

The interaction of bacteria with particulate organic matter has implications for organic matter cycling and bacterial ecology in the ocean. Until recently, the focus has been on 'classical' particles visible by unaided eye (marine snow) or light microscopy. Recent discoveries of several new types of abundant particles, from sub-micrometer to sub-marine snow, are changing our ideas of the physical and chemical nature of the particle field with which pelagic bacteria interact. Previous workers have discovered polysaccharide-containing (Alcian Blue stainable) transparent exopolymer particles (TEP) ranging from 3 to 100s of micrometers. Looking for additional components of the sub-marine snow particle field, …


Integrating Technology, Field Experience And Collaboration In An Undergraduate Biology Research Course, Melissa M. Grigione, Dan Farkas Oct 2012

Integrating Technology, Field Experience And Collaboration In An Undergraduate Biology Research Course, Melissa M. Grigione, Dan Farkas

Cornerstone 3 Reports : Interdisciplinary Informatics

No abstract provided.


Stomatal Conductance Trends Of The Jade Plant (Crassula Ovata) In Relation To Circadian Rhythm Entrainability, Katie Cahoon, Martin Garcia, Matthew Pierga Oct 2012

Stomatal Conductance Trends Of The Jade Plant (Crassula Ovata) In Relation To Circadian Rhythm Entrainability, Katie Cahoon, Martin Garcia, Matthew Pierga

Featured Research

The effect of an inverse light cycle on a Crassulacean acid metabolism (CAM) plant was observed in the study. CAM plants are unique in that they open their stomata at night in order to conserve water, an evolution that has come about because these plants primarily exist in very arid climates. By placing a plant in a chamber in which the lights could be programmed to turn on when it was dark out, and to turn off during normal daylight hours, the stomatal conductance of the leaves of a jade plant (Crassula ovata) were recorded several times a day over …


Is There A Correlation Between Water Potential And Mechanical Strength Of Stems Of Malosma Laurina?, William Chang, Lauren Price, Nicolas Pak Oct 2012

Is There A Correlation Between Water Potential And Mechanical Strength Of Stems Of Malosma Laurina?, William Chang, Lauren Price, Nicolas Pak

Featured Research

The purpose of this experiment was to see if there was a correlation between water potential and the stem mechanical strength of the Malosma laurina. This experiment was conducted with the use of a Scholander-Hammel pressure chamber to find water potential and the Instron 5500 to test the mechanical strength of the stems. After analysis of the data, there was no direct correlation between water potential and the mechanical strength of stems of Malosma laurina.


Photosynthetic Advantage Of Invasive Species, Gabby Gurule-Small, Alis Sokolova, Patrick Stephens Oct 2012

Photosynthetic Advantage Of Invasive Species, Gabby Gurule-Small, Alis Sokolova, Patrick Stephens

Featured Research

Californians have greatly benefited from the introduction of plant and animal species necessary for food or other human pursuits; however, there are many other introduced species that can wreak havoc on the state’s environment and economy. Invasive species threaten the diversity and abundance of native species by both competing for resources and causing changes to the natural habitat. We hypothesize that invasive species will have higher photosynthetic and conductance rates which contribute to their success. Through their impacts on natural ecosystems, agricultural lands, and water delivery systems, invasive species may also negatively affect human health and possibly even the economy.


The Effect Of Riparian And Arid Environments On Stomatal Conductance In Baccharis Salicifolia And Heteromeles Arbutifolia, Danalit Rangel, Vicki Mac, Ariel Lan Oct 2012

The Effect Of Riparian And Arid Environments On Stomatal Conductance In Baccharis Salicifolia And Heteromeles Arbutifolia, Danalit Rangel, Vicki Mac, Ariel Lan

Featured Research

A riparian environment is characterized by higher moisture levels than an arid environment; therefore they have different species of plants that can adapt to their natural habitats. It is critical that we explore the characteristics plants have in relation to their native environments. We propose to test the hypothesis that Baccharis salicifolia would have a higher stomatal conductance rate to water vapor loss compared to Heteromeles arbutifolia because Baccharis salicifolia thrives in a riparian environment in which water abundance would increase stomatal opening thereby contributing to the greater conductance. Using the LI-6400, we measured the conductance rate, photosynthetic rate, CO2 …


Effect Of Ambient Air Temperature On Leaf Size In Raphanus Sativus, Matthew Fleming, Michael Mccormack Oct 2012

Effect Of Ambient Air Temperature On Leaf Size In Raphanus Sativus, Matthew Fleming, Michael Mccormack

Featured Research

Global warming is an increasing problem in today’s society. Thus, it is important to know how plants, specifically those that are cultivated for human consumption, react to rising average temperatures. This experiment tested how the leaf size of a common crop plant, Raphanus sativus (radish), is affected by two different temperatures. It is hypothesized that as temperature rises, the leaf size will increase, giving the leaf greater ability to cool via transpiration because of an larger surface area for stomatal conductance. This hypothesis was tested by growing two samples of plants in separate incubators. One sample was grown at a …


The Relationship Between Leaf Mechanical Strength And Photosynthetic Rates, Aaron Van Loon, Brianna Manes, Taylor Stucky, Thomas Addington Oct 2012

The Relationship Between Leaf Mechanical Strength And Photosynthetic Rates, Aaron Van Loon, Brianna Manes, Taylor Stucky, Thomas Addington

Featured Research

Photosynthesis is the process by which plants convert light energy to chemical energy, helping sustain life in the biosphere. There are many factors that affect the rate of photosynthesis such as atmospheric CO2 concentration, temperature, and sunlight. In this experiment, we attempt to determine what effect, if any, the mechanical strength of a plant has on its rate of photosynthesis. To perform this experiment we used both the LI-COR 6400 and the Instron Mechanical Testing Device. With the LI-COR we measured photosynthetic rate of our plant subjects in real time during the prime photosynthetic hours, 10-12am. The Instron allowed us …


The Effect Of Dirt On Inhibition Of Light Absorption In Musa Leaves, Lorelle Knight, Alexis Carrington, Angela French, Roxanne Barker Oct 2012

The Effect Of Dirt On Inhibition Of Light Absorption In Musa Leaves, Lorelle Knight, Alexis Carrington, Angela French, Roxanne Barker

Featured Research

Dirt can be transported by wind, human activity and many other factors. It was hypothesized that dirt particles collected on leaves will decrease leaf reflectance and thus make the leaf less healthy. The rationale for this is that the more dirt present on the leaf, the more inhibited photons will be in reaching leaf pigments. The leaf will therefore be less healthy as it will be less able to perform photosynthesis. To test this hypothesis, eight leaf samples with varying amounts of dirt present, were collected from the plant, Musa. A Unispec spectrophotometer was used to test the reflectance of …


Lifelines Fall 2012, Southern Adventist University Oct 2012

Lifelines Fall 2012, Southern Adventist University

Lifelines - Biology Department Newsletter

The Fall 2012 issue of Lifelines features articles on undergraduate research in biology, field ecology, and the addition of Dr. Aaron Corbit to the faculty.


The Relationship Of Stomatal Conductnace To Mechanical Strength In Leaves Of Santa Monica Plants, Russell P. Mott, Steven R. Fleming, John R. Macbeth Oct 2012

The Relationship Of Stomatal Conductnace To Mechanical Strength In Leaves Of Santa Monica Plants, Russell P. Mott, Steven R. Fleming, John R. Macbeth

Featured Research

The Santa Monica Mountains ecosystem has a high diversity of plants with different lifestyles that produce different physiological characteristics individual to all plants. Studies in Australia, another Mediterranean ecosystem, have shown that mechanical strength of leaves is relatable to soil stress. This experiment seeks to determine whether mechanical strengths of leaves correlate to stomatal conductance of leaves across different species in the Santa Monica Mountains. Four species of plants are tested for their stomatal conductance in the field, and the leaves are tested for tensile strength using Young’s Modulus for comparison across leaves. These data show that there was no …


The Effect Of Leaf Trichome Density On Stem Mechanical Strength In Salvia Leucophylla, S. Mellifera, And S. Apiana, Brieanna English, Jeff Scanlon, Anushree Mahajan Oct 2012

The Effect Of Leaf Trichome Density On Stem Mechanical Strength In Salvia Leucophylla, S. Mellifera, And S. Apiana, Brieanna English, Jeff Scanlon, Anushree Mahajan

Featured Research

Salvia species in southern California exhibit a variety of leaf trichome densiIes. S. mellifera, S. leucophylla, and S. apiana were chosen as study organisms because they exhibit varying trichome densiIes. A UniSpec was used to measure NDVI in leaves and an Instron was used to measure stem mechanical strength. This study provides preliminary evidence that suggests plants with high leaf trichome density have less stem mechanical strength, and those with low leaf trichome density have greater mechanical strength. Data generated from this study supports the idea that this may be a general and loose trend amongst plant families similar to …


A Comparison Of Brittlebush (Encelia Farinosa) Productivity And Health During Drought And Post Rainfall, Gabi Diciolli, Karre Lawson, Anders Reimer Oct 2012

A Comparison Of Brittlebush (Encelia Farinosa) Productivity And Health During Drought And Post Rainfall, Gabi Diciolli, Karre Lawson, Anders Reimer

Featured Research

Encelia farinosa is well adapted for drought tolerance as its leaves transform color based on water availability. We were puzzled by a question that any desert botanist must consider. How does the photosynthetic efficiency of E. farinosa fluctuate as it transitions from a state of drought to a state of soil saturation within a 72 hour period? In this experiment we looked at the effects of irrigation on the health and quantum yield of the brittlebush by looking at photosynthetic rates using the portable photosynthesis system LI-COR 6400 when applied to two leaves on a given plant and water potential …


Characterization Of The Stem Cell Niche In Drosophila Testes, Vittorio Mena Jr. Oct 2012

Characterization Of The Stem Cell Niche In Drosophila Testes, Vittorio Mena Jr.

Seton Hall University Dissertations and Theses (ETDs)

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Achieving High Accuracy Prediction Of Minimotifs, Tian Mi, Sanguthevar Rajasekaran, Jerlin Camilus Merlin, Michael R. Gryk, Martin Schiller Sep 2012

Achieving High Accuracy Prediction Of Minimotifs, Tian Mi, Sanguthevar Rajasekaran, Jerlin Camilus Merlin, Michael R. Gryk, Martin Schiller

Life Sciences Faculty Research

The low complexity of minimotif patterns results in a high false-positive prediction rate, hampering protein function prediction. A multi-filter algorithm, trained and tested on a linear regression model, support vector machine model, and neural network model, using a large dataset of verified minimotifs, vastly improves minimotif prediction accuracy while generating few false positives. An optimal threshold for the best accuracy reaches an overall accuracy above 90%, while a stringent threshold for the best specificity generates less than 1% false positives or even no false positives and still produces more than 90% true positives for the linear regression and neural network …


Endoplasmic Reticulum Involvement In Yeast Cell Death, Nicanor Austriaco Aug 2012

Endoplasmic Reticulum Involvement In Yeast Cell Death, Nicanor Austriaco

Biology Faculty Publications

Yeast cells undergo programed cell death (PCD) with characteristic markers associated with apoptosis in mammalian cells including chromatin breakage, nuclear fragmentation, reactive oxygen species generation, and metacaspase activation. Though significant research has focused on mitochondrial involvement in this phenomenon, more recent work with both Saccharomyces cerevisiae and Schizosaccharomyces pombe has also implicated the endoplasmic reticulum (ER) in yeast PCD. This minireview provides an overview of ER stress-associated cell death (ER-SAD) in yeast. It begins with a description of ER structure and function in yeast before moving to a discussion of ER-SAD in both mammalian and yeast cells. Three examples of …


Condensin Ii Promotes The Formation Of Chromosome Territories By Inducing Axial Compaction Of Polyploid Interphase Chromosomes, Christopher R. R. Bauer, Tom A. Hartl, Giovanni Bosco Aug 2012

Condensin Ii Promotes The Formation Of Chromosome Territories By Inducing Axial Compaction Of Polyploid Interphase Chromosomes, Christopher R. R. Bauer, Tom A. Hartl, Giovanni Bosco

Dartmouth Scholarship

The eukaryotic nucleus is both spatially and functionally partitioned. This organization contributes to the maintenance, expression, and transmission of genetic information. Though our ability to probe the physical structure of the genome within the nucleus has improved substantially in recent years, relatively little is known about the factors that regulate its organization or the mechanisms through which specific organizational states are achieved. Here, we show that Drosophila melanogaster Condensin II induces axial compaction of interphase chromosomes, globally disrupts interchromosomal interactions, and promotes the dispersal of peri-centric heterochromatin. These Condensin II activities compartmentalize the nucleus into discrete chromosome territories and indicate …


Sesquiterpene Synthase Gene And Protein, Joe Chappell, Bryan Greenhagen Jun 2012

Sesquiterpene Synthase Gene And Protein, Joe Chappell, Bryan Greenhagen

Plant and Soil Sciences Faculty Patents

The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention.


Minor Fibrillar Collagens, Variable Regions Alternative Splicing, Intrinsic Disorder, And Tyrosine Sulfation, Ming Fang, Reed B. Jacob, Owen Mcdougal, Julia Oxford Jun 2012

Minor Fibrillar Collagens, Variable Regions Alternative Splicing, Intrinsic Disorder, And Tyrosine Sulfation, Ming Fang, Reed B. Jacob, Owen Mcdougal, Julia Oxford

Biomolecular Research Center Publications and Presentations

Minor fibrillar collagen types V and XI, are those less abundant than the fibrillar collagens types I, II and III. The alpha chains share a high degree of similarity with respect to protein sequence in all domains except the variable region. Genomic variation and, in some cases, extensive alternative splicing contribute to the unique sequence characteristics of the variable region. While unique expression patterns in tissues exist, the functions and biological relevance of the variable regions have not been elucidated. In this review, we summarize the existing knowledge about expression patterns and biological functions of the collagen types V and …


Electrical Advantages Of Dendritic Spines, Allan T. Gulledge, Nicholas T. Carnevale, Greg J. Stuart Apr 2012

Electrical Advantages Of Dendritic Spines, Allan T. Gulledge, Nicholas T. Carnevale, Greg J. Stuart

Dartmouth Scholarship

Many neurons receive excitatory glutamatergic input almost exclusively onto dendritic spines. In the absence of spines, the amplitudes and kinetics of excitatory postsynaptic potentials (EPSPs) at the site of synaptic input are highly variable and depend on dendritic location. We hypothesized that dendritic spines standardize the local geometry at the site of synaptic input, thereby reducing location-dependent variability of local EPSP properties. We tested this hypothesis using computational models of simplified and morphologically realistic spiny neurons that allow direct comparison of EPSPs generated on spine heads with EPSPs generated on dendritic shafts at the same dendritic locations. In all morphologies …


Teaching Stress Physiology Using Zebrafish (Danio Rerio), Michael Cooper, Shree Dhawale, Ahmed Mustafa Apr 2012

Teaching Stress Physiology Using Zebrafish (Danio Rerio), Michael Cooper, Shree Dhawale, Ahmed Mustafa

Ahmed Mustafa Dr.

A straightforward and inexpensive laboratory experiment is presented that investigates the physiological stress response of zebrafish after a 5 °C increase in water temperature. This experiment is designed for an undergraduate physiology lab and allows students to learn the scientific method and relevant laboratory techniques without causing significant stress to animals. An additional experimental design and a set of additional questions for lab report are also included.


A Unified View Of Base Excision Repair, Karen Almeida, Robert Sobol Apr 2012

A Unified View Of Base Excision Repair, Karen Almeida, Robert Sobol

Karen H Almeida

Base excision repair (BER) proteins act upon a significantly broad spectrum of DNA lesions that result from endogenous and exogenous sources. Multiple sub-pathways of BER (short-path or longpatch) and newly designated DNA repair pathways (e.g., SSBR and NIR) that utilize BER proteins complicate any comprehensive understanding of BER and its role in genome maintenance, chemotherapeutic response, neurodegeneration, cancer or aging. Herein, we propose a unified model of BER, comprised of three functional processes: Lesion Recognition/Strand Scission, Gap Tailoring and DNA Synthesis/Ligation, each represented by one or more multiprotein complexes and coordinated via the XRCC1/DNA Ligase III and PARP1 scaffold proteins. …


Interaction Of Fish Aryl Hydrocarbon Receptor Paralogs (Ahr1 And Ahr2) With The Retinoblastoma Protein, Rebeka Merson, Sibel Karchner, Mark Hahn Apr 2012

Interaction Of Fish Aryl Hydrocarbon Receptor Paralogs (Ahr1 And Ahr2) With The Retinoblastoma Protein, Rebeka Merson, Sibel Karchner, Mark Hahn

Rebeka Rand Merson

The aryl hydrocarbon receptor (AHR) mediates the toxic effects of 2,3,7,8-tetrachlorodibenzo-pdioxin (TCDD) and related compounds. In some mammalian cell lines, TCDD induces G1 cell cycle arrest, which depends on an interaction between the AHR and the retinoblastoma tumor suppressor (RB). Mammals possess one AHR, whereas fishes possess two or more AHR paralogs that differ in the domains important for AHR-RB interactions in mammals. To test the hypothesis that fish AHR paralogs differ in their ability to interact with RB, we cloned RB cDNA from Atlantic killifish, Fundulus heteroclitus, and studied the interactions of killifish RB protein with killifish AHR1 and …


The Effects Of Dependent Infants On The Social Behavior Of Mantled Howler Monkeys (Alouatta Palliate), Keziah Katz Apr 2012

The Effects Of Dependent Infants On The Social Behavior Of Mantled Howler Monkeys (Alouatta Palliate), Keziah Katz

Honors Theses and Capstones

Six weeks of observation on eight groups of wild mantled howler monkeys revealed that the presence of a dependent infant alters the frequency of social interactions between a female with an infant and other adult monkeys. Males preferred to spend time with females without infants but still spent more time with females with infants than with males. Females without infants spent significantly less time with females with infants than with males or other females without infants and females with infants decreased their frequency of social interaction overall without preference for males, females or other females with infants.


Cloning And Characterization Of The Cell Wall Acting Enzyme Cd1034 From The Pathogen Clostridium Difficile, Zachary Suter Apr 2012

Cloning And Characterization Of The Cell Wall Acting Enzyme Cd1034 From The Pathogen Clostridium Difficile, Zachary Suter

Honors Projects in Science and Technology

The manifestation of multidrug resistance in bacteria over the past several decades has resulted in one of the foremost challenges in the management of infectious diseases. The question arises, “How do we address this growing problem?” One solution to stem the growing rise in antimicrobial resistance is to investigate new targets, while another approach is to re-examine classical antibacterial targets with a fresh perspective. The aim of this paper is to begin the process of antibacterial development for the pathogen Clostridium difficile by characterizing the cell wall acting glucosaminidase CD1034. It is inunderstanding how CD1034 functions biochemically that it can …