Life Sciences Commons^™

Fire Management Of Spinifex Pastures In The Coastal And West Pilbara, John Stretch

Agriculture reports

Spinifex dominated plant communities are a major natural pasture resource in the Pilbara. Spinifex is noted for its ridged, hard and generally sharp pointed leaf blades, although individual species vary in this respect. Triodia pungens and T. schinzii are often referred to as soft spinifex. Soft spinifex is useful as stock feed in its own right, whereas 'hard' spinifex species are rarely grazed by stock. Soft spinifex dominated pastures are relatively uncommon in eastern parts but important on the western coastal plains of the Pilbara (Figure 1). Information on spinifex management options available to the pastoralist or station manager is …

Characterization Of P39, A Borrelia Burgdorferi Specific Protein, Jill M. Troyer

Theses and Dissertations in Biomedical Sciences

Borrelia burgdorferi, the causative agent of Lyme disease, expresses a species specific, 39 kiloDalton protein of unknown function called P39. This protein is highly immunogenic and the presence of anti-P39 antibodies in patient sera is used as an indicator of B. burgdorferi infection. P39 is also a candidate for vaccine developement due to conserved expression of P39 among B. burgdorferi isolates. Since little is presently known about this important molecule, the present study was designed to characterize P39 and was carried out in six separate investigations. First, infectious and noninfectious isolates of B. burgdorferi were established in vitro, and …

The Diet Of Oryzomys Palustris Based On Stomach Content Analysis, Shannon L. Wright

Biological Sciences Theses & Dissertations

Oryzomys palustris (Harlan) is unusual in that it is one of two carnivorous mammals in the Family Muridae in North America. A diet analysis of Oryzomys palustris. the marsh rice rat, was done based on stomach contents. This was accomplished by taking the animals from two study areas located on the Eastern Shore of Virginia. The animals were taken monthly for one year. Once caught, the animals were sacrificed and their stomach contents were identified to the lowest taxonomic level possible. This study showed that rice rats were omnivorous mammals, eating a variety of foods, including dicots, monocots, crabs, …

Brown-Headed Cowbird (Molothrus Ater) Range Expansion Into The Southeast: Effects On The Passerine Populations Using North American Breeding Bird Survey Data, Elizabeth Pruitt Henderson

Biological Sciences Theses & Dissertations

North American Breeding Bird Survey data that were collected from 1971-1992 in Virginia, North Carolina, south Carolina, Georgia, Florida, Alabama and Tennessee were used in this analysis of the impact of Brown-headed Cowbird range expansion on four warbler and four vireo host species.

Maps of mean cowbird abundances were produced using kriging for the periods 1971-1975, 1976-1980, 1981-1985, 1986-1990 and 1991-1992. These maps illustrated a complex invasion of the southeastern United States by Brown-headed Cowbird.

Linear regression analyses of host and cowbird abundances within physiographic strata, showed no species to be in decline or increasing in abundance over the entire …

Breeding Birds And Associated Upland Habitats Of Sherburne National Wildlife Refuge, Kent Sundseth

Culminating Projects in Biology

Sherburne National Wildlife Refuge supports a variety of upland habitats. These upland habitats may be divided into four main types: oak dominated woodlands, oak savanna, grassland and conifer plantations. Each of these habitats occupies a location along a vegetation gradient which begins with open grassland and ends with a stand of densely planted conifers. As the vegetation changes along the gradient so do the species of birds breeding there.

During the spring and summer months of 1994 -1996, a network of 73 survey points was established in the four main habitat types across the refuge. In 1995 and 1996 these …

Secondary Electron Emission From Simple Metals: Comparative Studies For Al, Mg, And Be, M. Rosler

Scanning Microscopy

In the secondary electron emission (SEE) from solids, the role of different excitation processes is now as ever of special interest from both the theoretical and the experimental points of view. Depending on the primary energy, the relative importance of different excitation mechanisms related to conduction as well as core electrons will be discussed for different simple metals. So far, first principles results are available only for Al for primary energies up to 10 keV. Starting from a microscopic description of the SEE based on the transport equation formalism, calculations were performed for other nearly-free-electron metals (Mg, Be) up to …

Spiranthes Cernua (L.) Rich., William Davison

Specimens by Name

No abstract provided.

Proceedings Of The International Conference On Genetic Improvement Of Sorghum And Pearl Millet, Darrell Rosenow, Gary Peterson, John Mullet, Henry Nguyen, Gebisa Ejeta, John Axtell, David Andrews, Jeff Dahlberg, A. Bruce Maunder, Kay Porter, Mike Gilbert, John Witcombe, John Stenhouse, C. Tom Hash, Kanayo Nwanze, Sam Mukuru, Anand Kumar, Francisco Gomez, Aboubacar Toure, Ouendeba Botorou

INTSORMIL Impacts and Bulletins

In 1971, an international symposium, "Sorghum in the Seventies", organized by the All India Coordinated Sorghum Improvement Project with support from the Indian Council of Agricultural Research and the Rockefeller Foundation was held in Hyderabad, India. The symposium reviewed the current knowledge base of the scientific, production and nutritional aspects of sorghum as a crop and as a human food. In 1981, ICRISAT, INTSORMIL, and the Indian Council of Agricultural Research (ICAR) sponsored "Sorghum in the Eighties", an international symposium at ICRISAT Center in India, to review the achievements accomplished in sorghum research during the preceding 10 years. They reviewed …

Hexapod Herald - Vol. 8, No. 13, September 27, 1996

Hexapod Herald and Other Entomology Department Newsletters

Grants

Congratulations

Office news

Welcome

Calendar of events

Deadlines

Simultaneous Identification Of A Specific Gene Protein Product And Transcript Using Combined Immunocytochemistry And In Situ Hybridization With Non-Radioactive Probes, Gwen V. Childs

Scanning Microscopy

Simultaneous identification of messenger RNA (mRNA) and proteins in the same cells or tissues is a valuable tool to help the cell biologist evaluate the cell secretory cycle. Some cells may produce the mRNA and delay the production of the proteins. Alternatively, the proteins may be rapidly secreted. Other cells may produce both in sequence within the same time frame. Because of this difference, some cells can only be identified by their mRNA product. Others may have both products. This presentation describes a non-radioactive approach to the detection of both products with dual-peroxidase labeling protocols in use in this laboratory …

Deposition Of Supercoiled Dna On Mica For Scanning Force Microscopy Imaging, B. Samori, I. Muzzalupo, G. Zuccheri

Scanning Microscopy

The deposition of DNA molecules on mica is driven and controlled by the ionic densities around DNA and close to the surface of the substrate. Dramatic improvements in the efficiency and reproducibility of DNA depositions were due to the introduction of divalent cations in the deposition solutions. The ionic distributions on DNA and on mica determine the mobility of adsorbed DNA molecules, thus letting them assume thermodynamically equilibrated conformations, or alternatively trapping them in non-equilibrated conformations upon adsorption.

With these prerequisites, mica does not seem like an inert substrate for DNA deposition for microscopy, and its properties greatly affect the …

Spirit Resting Places: A Nature Reader, Martin T. Thompson

MALS Final Projects, 1995-2019

When not involved in the matters of earning a living by teaching, I tramp the woods and fields to hunt or fish, or just to saunter through nature's mysterious beauty, and I read and write. That my writings embody reflections on my observations of the natural world is an extension of the self-discovery quest I have taken up. The pieces in this collection are mottled leafy pattern pieces that attempt to help me make sense of a life that began in rural Upstate New York. I was born and raised in the snow-belt region of Tug Hill, 15 miles east …

Fluorescence Imaging And Spectroscopy Of Biomaterials In Air And Liquid By Scanning Near-Field Optical/Atomic Force Microscopy, H. Muramatsu, N. Chiba, K. Nakajima, T. Ataka, M. Fujihira, J. Hitomi, T. Ushiki

Scanning Microscopy

We have developed scanning near-field optical/atomic force microscopy (SNOM/AFM). The SNOM/AFM uses a bent optical fiber simultaneously as a dynamic force AFM cantilever and a SNOM probe. Resonant frequency of the optical fiber cantilever is 15-40 kHz. Optical resolution of the SNOM/AFM images shows less than 50 nm. The SNOM/ AFM system contains photon counting system and polychrometer/intensified coupled charge devise (ICCD) system to observe fluorescence image and spectrograph of micro areas, respectively. Cultured cells were stained with fluorescein isothiocyanate (FITC)-labeled anti-keratin antibody or FITC-labeled phalloidin after treatment with Triton X-100. Fluorescence and topographic images were obtained in air and …

Electrical Characterization Of Submicrometer Silicon Devices By Cross-Sectional Contact Mode Atomic Force Microscopy, P. De Wolf, T. Trenkler, T. Clarysse, M. Caymax, W. Vandervorst, J. J. Snauwaert, L. Hellemans

Scanning Microscopy

Two contact mode atomic force microscopic (AFM) techniques under ambient conditions are presented for the electrical evaluation of cross sectioned silicon devices. In the first technique, a conductive AFM tip is used as a voltage probe to determine the local potential distribution on the cross section of a silicon device under operation. The electrical potential is measured simultaneously with the surface topography with nanometer resolution and mV accuracy, offering an easy way of correlating topographic and electrical features. A second method, nanometer spreading resistance profiling (nano-SRP), performs localized spreading resistance measurements to determine the spatial distribution of charge carriers in …

Nucleic Acid Detection By In Situ Molecular Immunogold Labeling Procedures, Marc Thiry

Scanning Microscopy

We have recently combined immunogold labeling procedures with molecular biology methods to pinpoint the precise locations of nucleic acids in biological material at the ultrastructural level. These new immunocytological approaches involve the incorporation of labeled nucleotides in the nucleic acids present at the surface of ultrathin sections prior to immunogold labeling. The antibodies used recognize a nucleoside analogue (bromodeoxyuridine) or a hapten (biotin) employed to label nucleotides. Examples of high-resolution detection include DNA or RNA present in different substructures of cell nuclei, and in particular, in adenovirus-induced intranuclear regions of HeLa cells. In addition to being highly sensitive and specific, …

Freeze-Dried Human Leukocytes Stabilized With Uranyl Acetate During Low Temperature Embedding Or With Oso4 Vapor After Embedding, L. Edelmann, A. Ruf

Scanning Microscopy

Two new simple stabilization procedures for freeze-dried biological material are introduced which are compatible with low temperature embedding (LTE) in Lowicryl. The first method uses a Lowicryl K11M/HM20 mixture supplemented with 0.3% uranyl acetate for LTE. For the second method polymerized Lowicryl blocks containing the freeze-dried material are exposed to OsO₄ vapor which penetrates into the Lowicryl block and stabilizes the embedded specimen. The quality of structural preservation is demonstrated with human leukocytes.

Towards In Situ Atomic Force Microscopy Imaging Of Biofilm Growth On Stainless Steel, D. T. Goddard, A. Steele, I. B. Beech

Scanning Microscopy

Atomic force microscopy (AFM) has been used to visualise the formation of bacterial biofilms on polished surfaces of 316 stainless steel. Imaging under ambient conditions revealed both the bacterial cells and the matrix of exopolymeric substances (EPS). These images exhibited good resolution with cell surface features as small as 30 nm distinguishable. In situ imaging was also carried out, and although the resolution was considerably reduced, images revealing the process of bacteria division have been obtained.

X-Irradiation-Induced Changes Of The Prelysosomal And Lysosomal Compartments And Proteolysis In Ht-29 Cells, Z. Somosy, A. Takáts, G. Bognár, A. L. Kovács, Á. Telbisz, A. Rácz, J. Kovács, G. J. Köteles

Scanning Microscopy

As a consequence of external and internal ionizing radiation, lysosome-like bodies have been observed to increase both in size and number in some cell types. We investigated this process by morphological methods (electron microscopy, cationized ferritin uptake, acid phosphatase histochemistry, morphometry) in cultured HT-29 cells. In parallel with these studies, we measured the rate of protein degradation on the basis of 14C-valine release from prelabeled cellular proteins. We found that at 2 and 4 Gy doses of X-irradiation the volume of the vacuolar (probably lysosomal) compartment increased without detectable changes of acid phosphatase activity. A 2 Gy irradiation dose did …

Ion And Electron Emission From Liquid Metal Sources, H. Niedrig

Scanning Microscopy

In liquid metal ion sources, the emission is located at the apex of a liquid cone (the often so-called Taylor cone), formed by electrostatic forces and surface tension. Reversal of the extraction voltage polarity results in electron emission from the liquid metal surface. For small apex radii, ≤ 1 μm, steady field emission of electrons has been observed, whereas for apex radii ≥ 10 μm, explosive pulsed emission occurs. Since the onset voltage for electron emission has been found to be considerably lower than the critical voltage for the formation of the Taylor cone, it has been concluded that dc …

Cropwatch No. 96-22, Sept. 20, 1996, Lisa Brown Jasa

Crop Watch

Inside

Evapotranspiration data ....... 150

Freeze outlook ...................... 151

Extension resources ............. 151

Turfgrass seed ...................... 152

Harvest safety ....................... 153

Precision farming conference ........................ 154

Open-Path Fourier Transform Infrared Studies Of Large-Scale Laboratory Biomass Fires, Robert J. Yokelson, David W. T. Griffith, Darold E. Ward

Chemistry and Biochemistry Faculty Publications

A series of nine large-scale, open fires was conducted in the Intermountain Fire Sciences Laboratory (IFSL) controlled-environment combustion facility. The fuels were pure pine needles or sagebrush or mixed fuels simulating forest-floor, ground fires; crown fires; broadcast burns; and slash pile burns. Mid-infrared spectra of the smoke were recorded throughout each fire by open path Fourier transform infrared (FTIR) spectroscopy at 0.12 cm⁻¹ resolution over a 3 m cross-stack pathlength and analyzed to provide pseudocontinuous, simultaneous concentrations of up to 16 compounds. Simultaneous measurements were made of fuel mass loss, stack gas temperature, and total mass flow up the …

A Comparison Of The Renal Structures Of The Anaconda And The Ball Python, H. Ditrich

Scanning Microscopy

The renal vascular system of the Ball python (Python regius) and the anaconda (Eunectes noteus; Serpentes - Squamata) has been investigated using light microscopy, transmission electron microscopy (TEM), and scanning electron microscopy (SEM) of vascular corrosion casts and critical-point dried non-corroded specimens. The average glomerular diameters of these two species differ significantly (anaconda: 59.1 μm, python: 124.3 μm). Also, the relative proportions of the renal tubules are different. These findings can be related to the different habitats of the two species (aquatic versus terrestrial environment).

What Can We Learn From Past Reform Efforts, R. Max Peterson

The National Forest Management Act in a Changing Society, 1976-1996: How Well Has It Worked in the Past 20 Years?: Will It Work in the 21st Century? (September 16-18)

7 pages.

Contains footnotes and references.

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Rural Communities In An Urban Society And Global Economy, Michael Preston

The National Forest Management Act in a Changing Society, 1976-1996: How Well Has It Worked in the Past 20 Years?: Will It Work in the 21st Century? (September 16-18)

8 pages.

Can Managers Adapt To New Relationships And Roles Under Nfma?, Elizabeth Estill

The National Forest Management Act in a Changing Society, 1976-1996: How Well Has It Worked in the Past 20 Years?: Will It Work in the 21st Century? (September 16-18)

5 pages.

Nfma Implementation Options: Managing Environmental Decisions [Outline], Susan Yonts-Shepard

The National Forest Management Act in a Changing Society, 1976-1996: How Well Has It Worked in the Past 20 Years?: Will It Work in the 21st Century? (September 16-18)

3 pages.

Nfma And Ecosystem Management, Richard L. Knight

The National Forest Management Act in a Changing Society, 1976-1996: How Well Has It Worked in the Past 20 Years?: Will It Work in the 21st Century? (September 16-18)

8 pages.

Contains 1 page of references.

The Need For A New Nfma Planning Rule, Jack Ward Thomas

The National Forest Management Act in a Changing Society, 1976-1996: How Well Has It Worked in the Past 20 Years?: Will It Work in the 21st Century? (September 16-18)

7 pages.

Global Trends And The Future Of National Forests, Nels C. Johnson

The National Forest Management Act in a Changing Society, 1976-1996: How Well Has It Worked in the Past 20 Years?: Will It Work in the 21st Century? (September 16-18)

23 pages.

Contains 3 pages of references.