Life Sciences Commons^™

Expression Of Α4Β1 Integrin On Tissue Eosinophils In The August Rat, John C. Phipps

Masters Theses

The integrin α4β1 is expressed on a wide variety of peripheral leukocytes, and functions in recruitment of these cells to sites of inflammaiton. We infected august (AUG) rats with the helminth parasite Nippostrongylus brasiliensis and later used leukocytes from the peritoneum of the sensitized animals and antibodies against α4β1 integrin to localize that adhesion molecule on the cell surfaces after antigen challenge. We found that after antigen challenge, eosinophils, monocytes, and small lymphocytes, but not mast cells, expressed α4β1 integrin if the animals had been previously infected, but cells from uninfected animals did not show expression of this molecule. Our …

Expression And Regulation Properties Of Pseudomonas L-Arabinose Utilization Plasmids, Mary E. Barecki

Masters Theses

The L-arabinose utilization pathway in Pseudomonas fluorescens is a unique metabolic pathway. In Pseudomonas, L-arabinose is converted to the end product α-ketogluterate and does not involve phosphorylated intermediates. This differs from the well studied L-arabinose pathway found in Escherichia coli which contains phosphorylated intermediates.

The enzymes of the L-arabinose pathway have been shown to be induced in the presence of L-arabinose in P. saccharophila. The current study determined regulatory properties of previously cloned chromosomal fragments of P. fluorescens which confer an L-arabinose positive phenotype to the surrogate host, Pseudomonas putida. Cells of P. putida containing plasmids pPZ221, …

The Effects Of Benzo(A)Pyrene And Dimethylbenzanthracene On Cultured Porcine Aortic Endothelial Function, Michelle Lynn Kagey

Masters Theses

Polycyclic aromatic hydrocarbons are environmental contaminants which have been shown to initiate and promote atherosclerosis and carcinogenesis in various animal species. These findings have been based primarily on the increase in incidence and size of smooth muscle cell tumors in these animals were exposed to benzo(a)pyrene (BaP) or dimethylbenzanthracene (DMBA). The endothelium has also been identified as an important mediator in vascular disease and atherogenesis, although the endothelial response to these PAHs has not been investigated. Thus, the focus of this study was on characterization of the endothelial response to BaP and DMBA. Concentration/response relationships for these chemicals were examined, …

Strength Properties Of Steam Exploded Laser Printed Papers, Dhamodharan Srinivasan

Masters Theses

The mam objective of this study was to investigate the effect of steam explosion pulping on the strength properties of recycled fibers. The properties considered were zerospan tensile strength, tensile breaking length, tensile stretch, tensile energy absorption, scott bond and pentosan content. This study also examined the important operating parameters that affect the properties. Laser printed waste paper was pulped by the steam explosion process. The experiments were statistically modeled to cover the various process variables. Chemical addition, residence time, pressure, and dispersant level were varied during the steam explosion experiments. Chemical addition was kept at four levels and other …

Comparison Of Forest Stand Edges In Riparian And Mesic Habitats Along Watts Bar Reservoir Shoreline, Ruth Anne Hanahan

Masters Theses

Approximately fifty years ago, the landscape upslope from the natural, riverine position banks of the Tennessee River was inundated by the closing of Watts Bar Dam. On the currently forested shoreline, habitats within the direct influence of the reservoir pool are riparian. Those above that influence are mesic. The purpose of this research was to determine compositional and structural differences between edges of mature forest stands established in a riparian habitat and those established in a mesic habitat along Watts Bar Reservoir shoreline.

Thirty quadrats were placed on shoreline sites with mature, minimally-disturbed forest stands: 15 in a riparian habitat …

The Flora And Ecology Of Seidman Park, Kent County, Michigan, Pamela J. Laureto

Masters Theses

A floristic study of Seidman Park, Kent County, Michigan was performed. The study identified 521 vascular plants belonging to 96 families and 284 genera. The plant communities of Seidman Park were delimited by identification of dominant species and observation of the physical environment. It was believed that communities existed within the upland forest that could not be identified by observation alone. The use of Non-Metric Multidimensional Scaling (NMS) ordination techniques identified three communities within the upland forest. These were a xeric community, a dry-mesic community and a nutrient-rich community. Three sub-communities of the dry-mesic community were also identified.

Effects Of Roads On Behavior And Survival Of Black Bears In Coastal North Carolina, David M. Brandenburg

Masters Theses

I studied the effects of roads on the behavior and survival of black bears (Ursus americanus) at Marine Corps Base Camp Lejeune in Coastal North Carolina from May 1990 to December 1992. I used mark-recapture methods, radiotelemetry, and a Geographic Information System (GIS) for the study.

I estimated there were 16 bears on the study area in 1990. By 1992, the population likely was reduced to ≤6 bears (3.3 bears/100 km²), primarily from vehicle-kills. Since 1988, vehicle-kills accounted for 20 of 28 (71 %) of the total known mortality. The annual female survival rate was 71 …

Dietary Staus Index: Association With Food Groups And Body Mass Index In Rural East Tennessee Women Living In Public Housing, Linda Lou Knol

Masters Theses

Dietary Status Index: Association with Food Groups and Body Mass

Index in Rural East Tennessee Women Living in Public Housing Objective: To determine if any differences existed in the number of servings per week consumed from designated food groups between women with low, medium or high Dietary Status Index (DSI) scores. Also, to determine if an association exists between Dietary Status Index (DSI) and Body Mass Index (BMI). DSI, a measure of both dietary adequacy and moderation, is the arithmetic mean of the Dietary Adequacy Score (DAS) and the Dietary Moderation Score (DMS).

Subjects: 121 non-pregnant females, 18 years or …

The Factors Influencing Electrographic Toner Flotation, Udaykumar Ratilal Raval

Masters Theses

Conventional deinking is the process of removing the ink from usable paper fiber. Electrographic toners are hydrophobic in nature because they have negligible negative charge. A study of the effect of fundamental properties leading to electrographic toner flotation in presence of free fibers has not been reported.

Flotation deinking of fused "sharp" toner fraction below 300 micron in the presence of free fibers was performed in the Hallimond tube. Cationic (Dodecyltrimethyl ammonium bromide - DTAB) and anionic (Sodium dodecyl sulfate - SDS) surfactants having similar molecular weights were used at two concentration levels and two pH levels. The effect of …

The Effects Of Fines, Drying Intensity And Recycling On Pulp And Paper Properties, Chiranjiv K. Singh

Masters Theses

The effects of fines level, drying intensity and recycling on pulp and paper properties of bleached softwood kraft were evaluated graphically and statistically.

Fiber fines (100-mesh) were mixed in proportions of 0, 10, 20, 40% with the long fiber fraction. These pulps were tested for fiber length distribution. Handsheets prepared from these pulps were dried at low (air dry) and high (220°F, 4.25 min) drying intensities. These handsheets were recycled and new handsheets were dried at the above drying levels.

Water retention values increased with increasing level of fines for virgin pulp. Recycling resulted in a 20% decrease in water …

Molecular Approaches In Attempts To Identify The Flagellin B-Type Gene In Clone Pkw52, Yolanda F. Kirkpatrick

Masters Theses

Pseudomonas aeruginosa is an important pathogen for susceptible individuals particularly those immunocompromised due to the prolonged use of drugs, noscomial infections, burns, or cancer. In addition, P. aeruginosa infections are high among cystic fibrosis patients. P. aeruginosa presents a unique health concern and challenge for researchers due to its multiple virulence factors and the increasing number of patients developing P. aeruginosa infections. P. aeruginosa produces a variety of toxins and enzymes. The flagellum of P. aeruginosa allows the bacterium to be motile. Motility has enabled the P. aeruginosa bacterium to rapidly colonize the host's body.

The major structural component of …

Identification, Life Cycle, And Presence Of Nectria Species Associated With Beech Bark Disease In The Great Smoky Mountains National Park And The Effectiveness Of Nematogonum Ferrugineum As A Biocontrol, Brenda A. Rutherford

Masters Theses

Two species of Nectria, N. galligena Bresadola and N. coccinea var. faginata Lohman, Watson, and Ayers, are associated with beech bark disease (BBD) in North America. In 1993, beech bark disease was discovered in the Great Smoky MOuntains National Park (GSMNP).

Ten sites for permanent plots were selected for incidence of the disease at different elevations in the GSMNP. These plots were rated four times in 1994 and 1995 for the presence of two scale insects, Cryptococcus fagisuga Lindinger and Xylococculus betulae (Pergande) Morrison, and the presence of perithecia of Nectria spp. The beech trees in each plot were also …

Analysis Of Free Sugars And Chlorophyll In Spinach From A Local Retail Market, Harvey J. Drews

Masters Theses

The accuracy (percentage recovery) and precision (coefficient of variation or CV) of extraction and an anion-exchange HPLC method using amperometric detection for measurement of free sugars in spinach were determined. Five samples of fresh spinach leaves were extracted and analyzed by the HPLC method for glucose, fructose and sucrose. Different amounts (0.093 to 0.453% of spinach, wet basis or WB) of each of the latter sugars were added to each of five spinach samples and these spiked samples were analyzed in a similar manner as the unspiked samples. The average percentage recoveries of glucose, fructose and sucrose were respectively, 106.6, …

The Hydrologic Characterization Of Three Forested Headwater Riparian Wetlands In East Tennessee, Mark Hale Eisenbies

Masters Theses

Headwater riparian wetlands form an important hydrologic link between terrestrial and aquatic systems. These wetlands are small and account for only nine percent of the total wetland area in the US, however, they account for nearly half of the upland/wetland edge. Three headwater riparian wetlands were instrumented with flumes, solution samplers and shallow wells to monitor hydrologic and chemical interactions between physiographic positions and streams within the watersheds. The objectives of this study were to examine the general hydrologic and chemical characteristics of such wetlands. Water sampling was conducted in soils and streams during baseflow and stormflow conditions. Cross sections …

The Effect Of Wet End Operations On Sizing Loss In Alkaline Papers, Suresh B. Cherukuri

Masters Theses

Changes in wet end of alkaline papermaking affect the size development as well as sizing loss. These changes include pH, alkalinity and type of filler used. Precipitated and ground varieties of carbonate fillers and the alkalinity associated with · them are believed to be the causes for the gradual sizing loss in alkaline papers due the accelerated hydrolysis reaction of sizing chemicals at higher alkalinity.

Precipitated and ground calcium carbonate fillers are used as fillers and compared to non carbonate filler such as kaolin clay for their response to size development and sizing loss at lower and higher alkalinity ranges …

The Effect Of Manganese Peroxidase On Peroxide Bleaching Of High Yield Pulps, Cyril Vusani Simelane

Masters Theses

The main objective of this study was to investigate the effect of adding a pretreatment stage in conventional hydrogen peroxide bleaching of high yield pulps. To achieve this, three main variables considered crucial in the effectiveness of manganese peroxidase on the oxidation of lignin substructures, were chosen to evaluate the effect of this enzyme on high yield pulps. The responses to evaluate this effect were brightness, brightness reversion, pulp viscosity, and yield. Other properties included opacity, scattering and absorption coefficients, and the Hunter Lab values.

The pulps were bleached using two sequences Q(MnP), and Q(MnP)QP, to assess the effect of …

The Role Of Nitric Oxide In The Modulation Of The Leukocyte-Endothelial Cell Interactions During Ischemia And Reperfusion, Fernando López-Neblina

Masters Theses

Nitric oxide (NO) is involved in several metabolic pathways and physiological phenomena. The role of NO in inflammation after ischemia and reperfusion (I/R) is controversial. The early phases of inflammation include the leukocyte-endothelial (L/EC) cell interactions which have been divided in rolling, activation, adhesion and migration.

The first experiment was to demonstrate the role of NO in a model of renal I/R. We demonstrated that exogenous NO improves survival and renal function tests after renal I/R, while endogenous NO does not appear to be an important contributor to renal I/R. We also demonstrated that NO modulate the infiltration of neutrophils …

Effects Of Insulin And Insulin-Mimetics On Mrna Levels Of C-Fos And C-Jun In Primary Rat Hepatocytes In Culture, Guangmin Li

Masters Theses

Insulin is capable of regulating cellular and metabolic processes as well as gene expression. Selenate and vanadate are two compounds that have been found to mimic the action of insulin in several tissue and cell types. cFos and cJun are the products of immediate early responsive genes c-fos and c-jun. They are members of the family of the AP-1 (Activator Protein-1) transcription factors. AP-1 transcription factors bind as cJun.cJun homodimer or cJun.cFos heterodimer to the TRE found in the promoter regions of several genes. AP-1 is characterized by its ability to alter gene expression in response to various peptide …

The Effect Of Age And Recycling On Paper Quality, Zhuang Wu

Masters Theses

The effects of recycling paper that has undergone aging for different periods on paper and fiber properties like strength, weighted average fiber length, and water retention value were investigated in this study. Old Newspapers (ONP) with different periods of natural aging (between 1 to 10 months) were recycled repeatedly for up to six times. Another goal was the study of aging effects on paper properties using accelerated aging at high temperatures (105±2) °C. Four different aging times (24, 48, 72, and 144 hours) were employed.

The results showed that naturally aged ONP loses little in strength properties up to 7 …

Comparison Of The Effects Of Aerobic Dance To Water Aerobic Training On Maximal Oxygen Consumption, Terri L. Bedford, Tamara A. Dusterwinkle, Darcy J. Hoppman

Masters Theses

Water aerobics is one mode of exercise that is gaining popularity. Water aerobics allows adults who participate in an exercise program to improve their cardiorespiratory fitness. The purpose of this study was to determine if water aerobic training is as effective as land dance aerobic training in improving cardiorespiratory fitness in sedentary healthy adults. Eighteen subjects participated in this study; nine in dance aerobics and nine in water aerobics. Before and after eight weeks of training, subjects performed a graded maximal exercise test on a Schwinn Air-Dyne while maximal oxygen uptake was measured via a Beckman Metabolic Cart. ANCOVA was …

Ecology And Community Relationships Of The River Cooter, Pseudemys Concinna In A Southern Illinois Backwater, Michael J. Dreslik

Masters Theses

In Illinois the River cooter, Pseudemys concinna, is an enigmatic endangered species. Even throughout its range, ecological studies on the River cooter are rare. During 1994 and 1995 I quantified the: growth rates and trends, population size and structure and dietary habits of a population from floodplain lake in Gallatin county, Illinois. Population estimation (Schnabel method) predicted 136 individuals at a density of 4.6 turtles/ha with a biomass of 3.6 Kg/ha. The sex ratio is slightly male biased (1:1.14). From back-calculated growth data, the von Bertalanffy growth model (Fabens' method) estimates males with a higher growth rate (k) and …

Electromyographic Comparison Of Internal And External Obliques Using A Modified Version Of Kendall's Strength Test Positions For Upper And Lower Abdominals, Rebecca A. Currier, Margie L. Johnson

Masters Theses

The purpose of this study was to examine the activity of the internal obliques (upper abdominals) versus the external obliques (lower abdominals) during a modified version of Kendall's upper and lower abdominal strength tests using surface electromyography on adults. The study was not correlated to Kendall's theory due to the necessity to have the internal oblique electrode placed on the anterior abdomen. Twenty-four women and sixteen men participated in the study. All subjects were taught two positions ("easy" and "hard") for both abdominal tests and performed eight trials. A normalized ratio was generated by dividing one "hard" trial by the …

Mechanistic Studies On Malate Dehydrogenase From Escherichia Coli, S. Kirk Wright

Masters Theses

Kinetic studies and chemical modification studies were performed on malate dehydrogenase from Escherichia coli (eMDH). Chemical modification studies using diethylpyrocarbonate and iodoacetic acid, along with log V/K profiles indicate that one ionizing group with a pK_a between 7.9 and 8.5 acts as a general acid/base in the catalytic mechanism. Log V profiles suggest that malate binds to the correctly protonated form of the enzyme. These results imply that a histidine residue is required for catalysis.

A primary deuterium kinetic isotope effect of 1.43 (±0.14) was observed on V/K using malate-2-d, while no isotope effect was measured on V_{max …}

Parental Investment In The Red-Eared Slider Turtle, Trachemys Scripta, Michael D. Marlen

Masters Theses

This study was conducted to determine egg and hatchling components of the red-eared slider (Trachemys scripta). In addition, energy components transferred from egg to hatchling were used to determine the level of parental investment in embryogenesis and hatchling care. Eggs of the red-eared slider, collected from central Illinois ponds, were obtained by inducing gravid females to lay by an injection of oxytocin. Egg and hatchling lipids were extracted with petroleum ether while egg and hatchling protein content was determined using the micro-Kjeldahl procedure. Eggs averaged 70.7% water by mass, and dry mass of whole eggs and egg yolks averaged 2.4g …

Physical Condition And Reproductive Success Of White-Tailed Deer At Fort Chaffee, Ar: A Five-Year Study, Donald A. Phillips

Masters Theses

A baseline study conducted in 1991 demonstrated that the resident white-tailed deer (Odocoileus virginianus) population at Fort Chaffee, Arkansas was in poor condition, with below average reproductive success, body weight, and antler size, and above average parasite load compared to other regional deer populations. Consequently, a management program was initiated in 1991 which included: (1) neutralizing acidic soils, (2) prescribed burning, (3) planting supplemental food plots, and (4) liberalizing the harvest of female adults from the population. The purpose of this study was to monitor changes in the health and reproductive success of the herd as this program was implemented. …

Small Mammal Community Structure In Restored Prairies, Kathleen A. Falout

Masters Theses

The Illinois Department of Natural Resources currently manages approximately 4,000 hectares of natural prairie. High human impacts coupled with small patch size result in challenging management decisions for prairie conservation. The ideal restored prairie would consist of a mosaic of habitats varying in vegetation from forb-grass dominated areas to oak savannas, with concurrent stages of vegetative succession occurring across the habitats. A study was conducted at the Middle Fork State Fish and Wildlife Area located in Vermillion County, IL, to analyze small mammal diversity and community composition among three different stages of prairie restoration.

The three treatment categories were: fallow …

Physiological Responses Among Power Air Ball, Spri Tube, And Arm Movement During Step Aerobics, Lisa Dallas

Masters Theses

This study compared caloric expenditure between three step aerobic dance routines (STEP) using the same arm movements; one without any upper body resistive exercise product (ARM), one with a 2 lb. Power Air Ball (BALL), and one with a Spri Tube (TUBE). Twenty-seven college aged females familiar with step aerobics volunteered to be subjects. Maximal oxygen uptake was measured using the Bruce protocol. A 55 minute video was followed for each of the STEP routines to control for arm and leg movement. The order of the STEP routines was randomly assigned. An Aerosport TEEM 100 gas analyzer calculated and recorded …

Growth Of Bluegill, Largemouth Bass, And Channel Catfish In Relation To Fish Abundances, Food Availability, And Other Limnological Variables, Sean P. Callahan

Masters Theses

Bluegill, largemouth bass, and channel catfish are widely distributed and popular sports species. We assessed growth of sympatric populations of these species in 14 reservoirs throughout Illinois, and attempted to relate growth to environmental factors. Principal components analysis (PCA) classified the lakes using 20 morphometric and limnological variables. We examined growth rate relationships with the principal components, via correlations, and also developed simple and multiple regression models using individual variables. Using size-specific growth for two size classes, percent littoral zone of a lake was correlated with growth of both channel catfish (300 and 450 mm) and small bluegill (50 mm). …

The Influence Of Environmental Ph And Bicarbonate Concentration On The Excretion Of Nitrogenous Waste Products In Two Species Of Freshwater Teleost, Paul D. Fabsits

Masters Theses

The catabolism of amino acids derived from dietary protein results in the production of ammonia and bicarbonate ion, waste products that an animal must eliminate. Often, animals excrete a nitrogenous waste product that is correlated with water availability: Freshwater fish, living where water is abundant, usually excrete ammonia while terrestrial animals excrete urea or uric acid. Recent studies report that fish inhabiting alkaline waters (pH 10) characterized by high bicarbonate concentrations do not excrete ammonia, instead synthesize urea, an energetically expensive process. The conversion to urea excretion by a normally ammonotelic animal is likely to be stimulated by a specific …

The Effects Of Group Selection On The Characteristics And Use Of Cavity Trees And Snags, Mary Lynn Lambert

Masters Theses

I studied the effects of group selection tree harvest on the availability, composition, and use of cavity trees, snags and cavities in the Ozark Mountains of northwestern Arkansas. Sixteen plots dominated by oaks and hickories that were 70-102 years old were surveyed prior to harvest. Tree species, diameter, height and number of visible cavities were recorded. In addition, 192 cavity trees were marked for wildlife observations during the summer and winter. Subsequently, plots were harvested by commercial contractors to either 15 or 19 m²/ha basal area, using either free or structural control thinning methods. A complete survey of …