Life Sciences Commons^™

Management Of Root Knot Nematode (Meloidogyne Incognita ) In Indiana Soybeans, David Edgardo Perla Martinez

Open Access Theses

The aim of this project was to evaluate different strategies for management of Root Knot Nematode (RKN) on soybean and tomato in Indiana. Seed treatments were evaluated under field and greenhouse conditions, but no effect on RKN populations was observed. Soybean lines evaluated for resistance to RKN under greenhouse conditions showed that six lines may be resistant to RKN. Four different commercial mustard cover crops were evaluated for their bio-fumigant impact on RKN populations in the production of tomato. Euruca sativa, Cv. Nemat was a poor host of RKN. A positive impact on the vigor of the tomato plants followed …

Differences In Carbon Dioxide Evolution From Samples Of Shelled Corn Subjected To Various Storage Treatments, Cininta Alp Pertiwi

Open Access Theses

Shelled corn was collected from two sources - commercial grain elevators and corn harvested from Purdue University farms and then frozen until tested. Using a carbon dioxide (CO₂) test kit manufactured by Woods End Laboratories, CO₂ evolution measurements were conducted on shelled corn from both sources, after the samples were re-wetted to approximately 21% moisture content, placed in a 473 ml glass jar, and incubated at room temperature for 72 hours. The CO₂ test kit uses a gel that changes color in response to a change in the level of CO₂ in the surrounding air. …

The Effects Of Radar On Avian Behavior: Implications For Wildlife Management At Airports, Eleanor R. Sheridan

Open Access Theses

Airports are areas with a high availability of resources for wildlife to forage, breed, and roost. Airports also have different types of radars to assist with air traffic control as well as tracking of wildlife that could become a risk for aircraft. The effect of radar electromagnetic radiation on wildlife behavior is not well understood. The goal of this study was to determine if bird behavior is affected by radar in two contexts: static radar (e.g., surveillance radar) and approaching radar (e.g., aircraft weather radar). We used brown-headed cowbirds as a model species. In the static radar context, we performed …

Abcb11 Functions With B1 And B19 To Regulate Rootward Auxin Transport, Jesica Elyse Reemmer

Open Access Theses

Auxin transport is essential for the architecture and development of erect plants. In a network of transporters directing auxin flows, ATP-Binding Cassette (ABC) transporters are a ubiquitous family of proteins that actively transport important substrates, including auxins, across the plasma membrane. ABCB1 and ABCB19 have been shown to account for the majority of rootward auxin transport, but residual fluxes to the root tip in Arabidopsis b1b19 double mutants implies the involvement of at least one additional auxin transporter in this process. Of specific interest, the severe dwarfism seen in abcb1abcb19 is strikingly reminiscent of that seen in mutants defective in …

Ems Induced Mutations In Dhurrin Metabolism And Their Impacts On Sorghum Growth And Development, Jenae Lavon Skelton

Open Access Theses

Sorghum is the fifth most important grain crop in the world. It is a staple food, feed, and silage crop in many developing countries in the semi-arid tropics. One factor that can impact sorghum forage quality is dhurrin content. Dhurrin is a cyanogenic glucoside naturally produced in the plant. When tissues containing dhurrin are crushed, hydrogen cyanide (HCN) is released during dhurrin decomposition. HCN is toxic to humans and livestock. While there is natural genetic diversity for the concentration of dhurrin within sorghum lines, there have been no naturally occurring dhurrin-free genotypes identified to date. ^ We have identified ethyl …

An Analysis Of Marketing Margins In The Us Beef Industry, John A. Tobin

Open Access Theses

The US beef industry has undergone significant structural changes in the last 30 years at the wholesale and retail levels. Meanwhile producer prices have failed to keep pace with increases in the price of beef received by wholesalers and retailers, resulting in concerns being raised about market power being exercised in the beef industry by stakeholders. ^ This study uses marketing margins to determine what factors affect margins, test for differences among different procurement methods of purchasing cattle, and test for differences among different cuts of beef. This paper extends previous research providing more detail regarding the factors that may …

Refining Phylogenetic Hypotheses Using Chloroplast Genomics And Incomplete Data Sets In Lasthenia (Madieae, Asteraceae), Joseph Frederic Walker

Open Access Theses

The genus Lasthenia (Madieae, Asteraceae), consists of predominantly annual plant species that are largely endemic to the California Floristic Province of western North America and occupy a large range of habitat types. With high levels of morphological and ecological diversity, Lasthenia is a robust tool, capable of providing a natural non-model organism for answering a diverse array of ecological and evolutionary questions. Future studies would benefit greatly from a strong phylogenetic hypothesis and more molecular resources, such as the whole plastome sequence for a representative species in the genus. Over a decade ago there was a study that laid a …

Effectiveness Of Field Days On Promoting The Adoption Of Recommended Improved Maize Varieties By Small Holder Farmers In Lilongwe District, Malawi, Yakosa Charity Zgawa Tegha

Open Access Theses

The study described the contribution of field days to promoting the adoption of improved maize varieties by small holder farmers of Chitsime and Mpingu Extension Planning Areas (EPAs) in Lilongwe District, Malawi. Sixty farmers from two EPAs where the field days had been conducted were randomly selected. Data were collected through personal interviews because literacy was a problem among farmers. The results of the study showed that 31 (51.7%) of the farmers interviewed were female. Use of other methods such as radio, mobile van, leaflets, posters, village meetings and messages sent to farmers’ cellphones helped to popularize the improved maize …

Iron Nutriture Following Roux-En-Y Gastric Bypass Surgery, Breanne N. Wright

Open Access Theses

Roux-en-Y gastric bypass surgery (RYGB) is effective for weight loss, but is commonly associated with iron deficiency and its clinical manifestation, anemia. Diagnosing iron deficiency is complex because iron status depends on other nutrients; additionally, anemia following surgery is not specifically due to deficiency in iron, as it can be due to deficiencies in other nutrients including zinc, copper, vitamin B₆, folate, and vitamin B ₁₂. In patients who have undergone RYGB, our aims were to 1) conduct a comprehensive assessment of nutrients involved in iron homeostasis, 2) determine the contribution of dietary intake to iron deficiency, …

Role Of Resveratrol Metabolites In Adipose Function, Mikaela Lynn Allan

Open Access Theses

The rise in obesity rate has drastically increased over the last few decades and has quickly grown into a worldwide epidemic. Large increases in visceral adipose tissue accumulation increase risk for metabolic disorders including insulin resistance, type 2 diabetes, and cardiovascular diseases. Currently, increased levels of circulating non-esterified fatty acids (NEFA) have been suggested to be the mediator linking obesity and type 2 diabetes. Studies identifying ways to alleviate mobilization of NEFA in adipose tissue via dietary phytochemicals may useful as a therapeutic approach. Despite the established benefits of resveratrol in health and in the development of obesity, the role …

Efficacy Of Cleaning Method For Removal Of Exogenous Welding Fume Contamination From Nail Tissue Prior To Use As A Biomarker For Welding Fume Manganese Exposure, Jeffrey Corral Bainter

Open Access Theses

Nail tissue has been proposed as a biomarker for body burden of occupational exposure to manganese from welding fumes. Though recent studies have shown correlation between manganese exposure and both nail tissue concentration as well as concentrations in dopaminergic regions of the brain, concerns of the validity of nail tissue as a biomarker have arisen due to the potential for exogenous contamination of Mn to undermine the quantization of endogenous Mn in nail. Previous studies have used a cleaning method of 1% Triton X-100 surfactant plus sonication in order to attempt to remove exogenous welding fume contamination. Determination of the …

Characterization Of Water-Solid Interactions In Crystalline Ingredients And Development Of Deliquescence Measurement Recommendations, Matthew C. Allan

Open Access Theses

There are five major mechanisms of water-solid interactions. The primary focus of this thesis was on two of these: deliquescence and hydrate formation. Many crystalline food ingredients are deliquescent compounds (e.g., NaCl, sucrose, and ascorbic acid) and some are both deliquescent and hydrate formers (e.g., glucose, thiamine HCl, citric acid). Deliquescence is the first order phase transformation of a crystalline solid to a solution above a critical relative humidity (RH) known as the deliquescence point (RH0). A crystalline hydrate is a pseudo-polymorph in which water is incorporated into the crystal structure, altering the molecular formula and the physical properties.^ To …

1,25-Dihydroxyvitamin D Alters Lipid Metabolism And Epithelialto- Mesenchymal Transition In Metastatic Epithelial Breast Cancer Cells, Alle Nicole Barnard

Open Access Theses

Evidence suggests that high vitamin D status (marked by serum 25-hydroxyvitamin D, 25(OH)2 D) is associated with a decreased risk of breast cancer. It has been established that 1,25-dihydroxyvitamin D (1,25(OH) 2 D) can alter glycolysis and the Krebs cycle of breast cancer cells (Jiang et al., 2010; Zheng et al., 2013) but little information is available on 1,25(OH) 2 D's alterations of lipid metabolism in breast cancer cells. Thus, the current research investigates if there was an effect of 1,25(OH) 2 D on proteins that regulate lipid metabolism in MCF10A, MCF10A-ras, MCF10CA1h, and MCF10CA1a epithelial breast cancer cells. While …

Evolution Of Food Quality Demand In The Food Service Industry In China: The Case Of Duck, Rachel Alison Carnegie

Open Access Theses

Booming economic growth and rising consumer incomes have impacted food preferences and purchasing behavior in China. At the same time, several internationally publicized food safety incidents, particularly in the animal husbandry sector, have heightened awareness of and concern for food safety and quality in meat and dairy. Rising quality demand and safety concerns have been studied at length in the food retail sector, but also appear to be important in the food service industry. This research uses data from a survey of duck restaurant managers and consumers in Beijing, Shanghai, Chengdu, and Guangzhou to determine preferences and willingness to pay …

High Voltage Atmospheric Cold Plasma Treatment Of Refrigerated Chicken Eggs For Control Of Salmonella Enteritidis On External Surfaces, Yi Chen

Open Access Theses

The global market for eggs is projected to reach 1.154 trillion by the year 2015. The number one risk to consumers from chicken eggs is contamination with Salmonella Enteritidis (SE). FDA estimates each year 142,000 illnesses in the U.S. are caused by SE contamination in chicken eggs. The first objective of this research study was to evaluate a novel High Voltage Atmospheric Cold Plasma (HVACP) technology in reducing externally inoculated SE on refrigerated chicken eggs. Raw, chicken eggs attached with SE population (10 7 CFU/ml), were placed in plastic containers filled with either dry Air (22%O2 , 78%N2 ) or …

Design, Development And Testing Of A Balance Board With Variable Torsional Stiffness And Time Delay, Denise Renee Cruise

Open Access Theses

The ability to balance and maintain upright posture can decline for a variety of reasons, such as aging and neuromuscular impairment. As the ability to balance declines, the risk of falling increases. Falls are a major cause of injury, and often lead to a dramatic decline in quality of life. Currently, to alleviate balance deficiencies, people participate in balance training, which most commonly refers to standing on an unstable balance board; the most common boards used are either passive wobble boards, or more advanced commercial systems such as the Biodex System SD ® or the Neurocom SMART Balance Master® . …

Agronomic And Genetic Characterization Of Aluminum Tolerance In A Recombinant Inbred Population Of Sorghum [Sorghum Bicolor (L.) Moench], Amanda Christine Easterly

Open Access Theses

Acid soils are estimated to cover up to 30% of arable soils globally and lead to significant limitations on agricultural productivity, primarily through aluminum toxicity. In sorghum [Sorghum bicolor (L.) Moench], significant genetic variation exists for tolerance to phytotoxic species of aluminum; tolerance is conferred through the exudation of citrate at the root tip, binding aluminum in the soil rhizosphere. A gene in the multidrug and toxic compound extrusion (MATE) family is the primary tolerance locus in sorghum. The purpose of this research was to evaluate a recombinant inbred line (RIL) population of sorghum segregating for SbMATE to establish …

Particle Swarm Optimization Using Multiple Neighborhood Connectivity And Winner Take All Activation Applied To Biophysical Models Of Inferior Colliculus Neurons, Brandon S. Coventry

Open Access Theses

Age-related hearing loss is a prevalent neurological disorder, affecting as many as 63% of adults over the age of 70. The inability to hear and understand speech is a cause of much distress in aged individuals and is becoming a major public health concern as age-related hearing loss has also been correlated with other neurological disorders such as Alzheimer's dementia. The Inferior Colliculus (IC) is a major integrative auditory center, receiving excitatory and inhibitory inputs from several brainstem nuclei. This complex balance of excitation and inhibition gives rise to complex neural responses, which are measured in terms of firing rate …

Managing Swine Manure In Double-Crop Soybean, Donald Joseph Graper

Open Access Theses

Animal production, especially swine (Sus scrofa domesticus ) and poultry, has increased in the United States prompting the need to manage the larger volume of manure beyond land applications to supply nitrogen (N) for corn (Zea mays ) production. Applying swine manure to soybean (Glycine max ) has been shown to increase grain yield due to N supply. The goal of this research was to determine if swine manure applications to double-crop soybean following wheat can limit manure N loading to the soil by extending management options, increase soybean biomass and grain production, and increase N removal …

Variation In Round Goby Feeding Interactions In Near-Shore Lake Michigan Based On Three Trophic Indicators, Michael Lee Henebry

Open Access Theses

Understanding trophic interactions of non-native species is key to elucidating their potential ecological role in newly-invaded ecosystems. The Great Lakes region has long been characterized by a high rate of invasive species establishment. Round Goby (Neogobius melanostomus ) were first detected in Lake Michigan in 1994, and have since increased dramatically in abundance. While past studies of Round Goby in Lake Michigan have primarily focused on specific regions and habitats, Lake Michigan is a large, diverse system and Round Goby trophic interactions may vary across regions and over time. During May, July, and September 2010-2011, we collected Round Goby …

Price Impacts Of Increased Peruvian Table Grape Supply, Jacob W. Iest

Open Access Theses

In the last decade, Peru has begun to structure its economy and complete infrastructure projects to promote agricultural export growth. Bolstered by the signing of the Peru Trade Promotion Agreement (PTPA) with the U.S., table grape production has increased significantly since 2006. Peru has a climatic advantage for a quality harvest from November to March, which overlaps the end of the U.S. growing season. With this supply increase expected to continue, U.S. producers and marketers question the effect Peru will have on "late-season" table grape prices in North America.

This thesis is a study of the effects of an increase …

Two Essays On Large Agricultural Producer Buying Behaviors, Jacqueline K. Holland

Open Access Theses

The trend of consolidation of large farms into high volume operations has continued over the past 30 years. Large farm management is a juggling act of a variety of biological processes, financial management, technology adaptation, and human resource development activities. This research looks to provide insights into the underlying management psychology of those large farmers to serve not only as a benchmark for other producers, but to provide customer information to agribusinesses so these firms can strengthen their relationships with their large farmer customers. The first part of this analysis will use Best-Worst modeling to examine five predetermined success factors …

The Combined Effects Of Anthropogenic And Environmental Stressors On Fundulus Grandis Development, Christopher Jacob Klinkhamer

Open Access Theses

The 2010 Deepwater Horizon oil spill resulted in the release of ~ 5 million barrels of crude oil from the Macondo wellhead into the Gulf of Mexico. Oil from the spill was documented on 1,772km of shoreline with 847km of shoreline remaining oiled a year after the spill, and 687km of shoreline remaining oiled two years after the spill. Currently, there are few data available concerning long-term, sublethal effects associated with chronic exposure to crude oil in the Gulf of Mexico. In addition to the anthropogenic-induced stress of polycyclic aromatic hydrocarbon (PAH) exposure, aquatic organisms living in estuarial areas of …

Using The Microsoft Kinect To Assess Human Bimanual Coordination, Joshua James Liddy

Open Access Theses

Optical marker-based systems are the gold-standard for capturing three-dimensional (3D) human kinematics. However, these systems have various drawbacks including time consuming marker placement, soft tissue movement artifact, and are prohibitively expensive and non-portable. The Microsoft Kinect is an inexpensive, portable, depth camera that can be used to capture 3D human movement kinematics. Numerous investigations have assessed the Kinect's ability to capture postural control and gait, but to date, no study has evaluated it's capabilities for measuring spatiotemporal coordination. In order to investigate human coordination and coordination stability with the Kinect, a well-studied bimanual coordination paradigm (Kelso, 1984, Kelso; Scholz, & …

Physical And Chemical Attributes Of A Genetically Modified Fruit Pectin, Carl Patrick Littrell

Open Access Theses

Pectin is an important polymer used in the food industry as a thickening and gelling agent. Though pectin is ubiquitous in plants, chemical and structural differences among pectin molecules prevent most from being viable for industrial use. Enzymes found naturally in fruit cell walls during the ripening process impair many desirable attributes of fruit pectins, rendering them unsuitable for industrial applications. Pectin methylesterase (PME) is one such enzyme whose expression can be altered during ripening through the use of recombinant genetic engineering. Reduction in levels of PME results in increased degree of methylation and molecular size of pectin, greatly increasing …

Habitat Effects On Chick-A-Dee Call Complexity, Jacqueline Renee Lynch

Open Access Theses

Past studies on the communication systems of species in urban environments (such as Common Starlings (Sturnus vulgaris), nightingales (Luscinia megarhynchos Brehm), brown-headed cowbirds ( Molothrus ater), Southern brown tree frogs (Litoria ewingii )) have shown multiple ways that species change vocal signaling behavior to adjust to urban habitats (e.g. alarm calls and singing). This study further investigates the changes in signaling in relation to the chick-a-dee call of the Carolina chickadee (Poecile carolinensis). A secondary goal of this study was to compare both the amount of information and rate of calling across …

Investigation Into The Control Of Melittin Secondary Structure And Antimicrobial Activity, Zachary B. Molinets

Open Access Theses

Antimicrobial resistance has been an exponentially growing problem since the discovery of antibiotics. Antibiotics have been misused for many years and this misuse has grown into a real problem for the medical community. While there are countless safeguards to prevent infection by a resistant strain of bacteria, there are still many plagued by it and must be treated with sometimes dangerous antibiotics. Melittin, along with many other peptides, contain potent antimicrobial properties, but are also toxic toward enthrocytes. The control of the secondary structure of peptides provides the key to adjusting their activity.

Key Residues Of Human Cytoplasmic Protein Tyrosine Phosphatase-A And -B For Substrate Binding And Specificity, Byunghyun Park

Open Access Theses

Reversible tyrosine phosphorylation plays an important role in signaling pathways that are essential for regulating cellular growth, differentiation and metabolism. Moreover, several human diseases such as diabetes, obesity and cancers are associated with the deregulation of protein tyrosine phosphatases (PTPs). Several studies provide evidence that PTPs not only contribute to cellular differentiation, but over-expression of these molecules also leads to transformation of non-transfomed cells as well. Based on these results, designing specific PTP inhibitors may ultimately function as potential therapeutic agents to treat various diseases including cancer, diabetes, and autoimmune diseases. EphA2 is a receptor tyrosine kinase which is hypo-phosphorylated …

Clinical Investigation Of Plethysmographic Variability Index: A Derivative Index Of Pulse Oximetry In Anesthetized Dogs, Vaidehi V. Paranjape

Open Access Theses

Plethysmographic Variability Index (PVI) is a derivative index of pulse oximetry that allows evaluating an individual's intravascular volume status. Perfusion Index (PI) represents the strength of pulse signal at the anatomic site of measurement from which PVI is calculated using changes in PI over respiratory cycles. Plethysmographic Variability Index has been used to detect hypovolemia and predict fluid responsiveness in mechanically ventilated human patients however, fewer studies are available in spontaneously breathing patients. The use of PVI has not been explored extensively in dogs so far. The goals of this study were to establish a common range for PVI and …

Field To Flight: A Techno-Economic Analysis Of Stover To Aviation Biofuels Supply Chain, Amanda C. Bittner

Open Access Theses

Greenhouse gas emissions have been a growing concern. The transportation sector contributes to one-third of GHG emissions in the United States from fossil fuel burning. The Renewable Fuel Standard set a requirement for 16 billion gallons (ethanol equivalent) of cellulosic biofuels to be used in the market. Aviation biofuels can help to meet both of these problems as well as improve U.S. energy security.

Investment in the biofuel industry carries a lot of risk. The biofuel industry is run by the private sector, but can be incentivized by government. Cellulosic biofuels carry even more risk than first generation biofuels, because …