Life Sciences Commons^™

The Impact Of An Omega-3 Enriched Diet On Hyperactivity And Biochemistry In An Animal Model For Attention-Deficit/Hyperactivity Disorder, Nadine M. Hammoud

Open Access Theses

Attention-deficit/hyperactivity disorder (ADHD) is the most diagnosed behavioral disorder in children. It affects around 5% of children worldwide and 11% of children in the United States, with rates increasing. Pharmaceutical treatments, such as amphetamines and methylphenidates, are not effective for everyone and are known to have unwanted side effects. While the etiology of the disorder is not yet fully understood, there are clear genetic and environmental components. Nutritional insufficiencies have recently become a popular environmental risk factor under investigation. Essential fatty acids (EFA), omega-3 polyunsaturated fatty acids (PUFA) in particular, are needed for proper brain development and function. Our lab …

Postural Sway In Infants At Low And High Risk For Autism Spectrum Disorder, Rachel C. Harris

Open Access Theses

ABSTRACT Harris, Rachel C. M. S., Purdue University, December 2014. Postural Control in Infants at Low and High Risk for Autism Spectrum Disorder. Major Professor: Laura J. Claxton. Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder more commonly known for deficits in social and communication skills. More recently, aspects of motor development have been shown to be delayed in children with ASD, including deficits in their postural control abilities. Children with ASD have difficulty integrating information from their sensory systems to help control their balance. Infants at risk for ASD and infants who are later diagnosed with ASD have delays …

Analysis Of Vitamin E Metabolites By Liquid Chromatography-Tandem Mass Spectrometry, Tianlin Xu

Open Access Theses

Naturally occurring forms of Vitamin E are metabolized to various carboxychromanols and conjugated carboxychromanols. Recent studies showed that vitamin E metabolites, especially the long-chain carboxychromanols are more bioactive than unmetabolized vitamin E forms. It is necessary to quantify vitamin E metabolites in biological environment. Here a simple and effective extraction method was developed to achieve extraction efficacy of more than 90% of various forms of vitamin E and metabolites with less than 10% inter- or intra-day variation. An LC-MS/MS assay was developed and optimized to acquire best sensitivity for the detection of vitamin E and metabolites. This method allows simultaneous …

1,25-Dihydroxyvitamin D Alters Lipid Metabolism And Epithelialto- Mesenchymal Transition In Metastatic Epithelial Breast Cancer Cells, Alle Nicole Barnard

Open Access Theses

Evidence suggests that high vitamin D status (marked by serum 25-hydroxyvitamin D, 25(OH)2 D) is associated with a decreased risk of breast cancer. It has been established that 1,25-dihydroxyvitamin D (1,25(OH) 2 D) can alter glycolysis and the Krebs cycle of breast cancer cells (Jiang et al., 2010; Zheng et al., 2013) but little information is available on 1,25(OH) 2 D's alterations of lipid metabolism in breast cancer cells. Thus, the current research investigates if there was an effect of 1,25(OH) 2 D on proteins that regulate lipid metabolism in MCF10A, MCF10A-ras, MCF10CA1h, and MCF10CA1a epithelial breast cancer cells. While …

Design, Development And Testing Of A Balance Board With Variable Torsional Stiffness And Time Delay, Denise Renee Cruise

Open Access Theses

The ability to balance and maintain upright posture can decline for a variety of reasons, such as aging and neuromuscular impairment. As the ability to balance declines, the risk of falling increases. Falls are a major cause of injury, and often lead to a dramatic decline in quality of life. Currently, to alleviate balance deficiencies, people participate in balance training, which most commonly refers to standing on an unstable balance board; the most common boards used are either passive wobble boards, or more advanced commercial systems such as the Biodex System SD ® or the Neurocom SMART Balance Master® . …

Fatty Acids And Mercury In Seventy Seven Species Of Commercially Available Finfish In The United States, Dennis P. Cladis

Open Access Theses

Finfish are consumed across the United States and constitute an important part of the American diet. However, seafood consumption can be a tenuous topic, with supporters highlighting the health benefits of eicosapentaenoic acid (EPA, C20:5n3) and docosahexaenoic acid (DHA, C22:6n3) and opponents emphasizing the neurotoxicity of methylmercury. Because all fish contain varying amounts of EPA, DHA, and methylmercury, the need for clear and unbiased information is essential to alleviate the confusion experienced by many consumers and empower them to make informed decisions regarding seafood consumption. As the market changes and more fish originate from aquaculture sources, where diets are controlled, …

Bridging The Omega-3 Gap: The Disparity Between Actual And Target Intakes Of Eicosapentaenoic Acid (Epa) And Docosahexaenoic Acid (Dha) In Children, Lyndsey Rae Herdzina-Huss

Open Access Theses

To improve children's diet quality for optimal growth and development and the prevention of chronic disease, we conducted two studies. One was focused on analyzing nationally representative data; the other was designed to test the feasibility of children accepting and consuming oily fish in a childcare setting. The objective of our first study was to provide evidence for 2-18 year old children's fish and shellfish consumption patterns and their eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) intake. The aims of this study were to estimate proportion of children consuming fish, to identify major dietary sources of EPA and DHA, and …

Physical Activity Informational Websites: Accuracy, Language Ease, And Fear Appeal, Samantha Rose Paige

Open Access Theses

Introduction. Health information is one of the most common searches on the Internet. Literature supports that, in general, health information readily available to Internet consumers is not accurate, lacks plain language for ease of understanding, and does not incorporate behavior-change theoretical frameworks. The purpose of this study was to evaluate each of these components. Method. Three keywords, "physical activity," "exercise," and "fitness," were entered into four popular search engines: Google, Bing, and Yahoo. The first 5 result pages were considered, totaling a sample of 493 websites. After exclusionary criteria, 102 websites were reviewed and analyzed with guidance from ACSM recommendations, …

Deforestation Of Cloud Forest In The Central Highlands Of Guatemala: Soil Erosion And Sustainability Implications For Q'Eqchi' Maya Communities, Ian Christopher Pope

Open Access Theses

Understanding the nexus between deforestation, food production, land degradation, and culture contributes knowledge that is useful for development practitioners working to enhance conservation and food security. Documenting deforestation and soil erosion in the Sierra Yalijux and Sierra Sacranix in the Central Highlands of Guatemala adds new knowledge about the rates and dynamics of deforestation and land degradation in areas with unique and sensitive cloud forest ecosystems. It also suggests possible areas of emphasis for efforts targeted at combining cloud forest conservation with sustainability for indigenous Q'eqchi' communities. In addition, this work contributes to a small but growing body of literature …

Neurotrophins And Their Effects On Breast Cancer Cell Proliferation And Migration, Kayla Elise Minser

Open Access Theses

Cancer is a large health issue in all parts of the world. In the United States alone, approximately 1 in 4 deaths are cancer related. Breast cancer is a particularly prevalent form, accounting for a little over 14 percent of all cancer incidence. The largest obstacle to overcome for breast cancer morbidity is metastasis. Over 90 percent of all breast cancer related deaths are due to metastasis. Because metastasis is a complex, multi-step process, it is difficult to treat. A recent observation in the Kirshner lab has revealed a type of phenotypic plasticity, where migratory cancer cells have a neuronal-like …

Consumer Perceptions Of Child-Friendly Shaped Healthy Fruit And Vegetable Snacks, Selena Lauren Baker

Open Access Theses

Fruits and vegetables (FV) are widely recognized as healthful foods by the public, and most individuals are aware of dietary guidance recommendations to consume more FV. However, actual consumption of FV has been and continues to be low in the United States and many other countries, despite public health efforts to change this trend. The sub-optimal intake of FV among children and adolescents is of particular concern due to high nutrient requirements for proper growth and development during these life stages. Fruit and vegetable intake patterns in childhood have been shown to track into later life and may affect individuals' …

An Economic Analysis Of Sizing Constraints: Price Discrimination And Changes In Welfare Under The New York City Soda Ban, Brian A. Bourquard

Open Access Theses

This thesis uses profit maximizing techniques and nonlinear, second-degree price discrimination theory to describe changes in consumption and gains and losses in welfare of consumption under a container size constraint. It observationally examines the New York City soda ban to provide insight into retailer behavior in the event of such a ban, and examines three cases to highlight potential retailer decisions to compensate for a ban: to continue using a restricted menu of pricing options; to sell only to high type soda consumers; or to serve both markets with a one-size-fits-all strategy. This thesis finds that the ban generally reduces …

An Exploratory Analysis Of Fruit And Vegetable Consumption In Black Men, Jacqueline Reiter

Open Access Theses

Objective: Multiple peer-reviewed studies have found an association between fruit and vegetable consumption and lower risk for diseases such as hypertension, stroke and cancer. In other related studies, education level, retail food environment, and fruit and vegetable consumption were also examined together to discover patterns and associations. Currently, Black males have a higher risk for poor health outcomes. Limited research has focused specifically on Black men's fruit and vegetable consumption. This study explored the association between education level, food store access (measured by proximity) and fruit and vegetable consumption in black African American men.

Design: The data for this study …

Efficacy Of Manual Dishwashing And Sanitizer Wiping In Removing Food Soils From Chel Knives, Xiaodi Sun

Open Access Theses

Improperly cleaned and sanitized chef knives may present a potential contamination risk and thus be a risk for foodborne illness. Therefore, ensuring effective cleaning and sanitization of knife surfaces may help prevent the spread of foodborne pathogens. The primary objective of this research was to compare the efficacies of two different cleaning methods (three-compartment manual dishwashing and sanitizer wiping) at removing food soils from contaminated chef knives using procedures that are typical of restaurant kitchens. Knife wiping and washing procedures were determined and standardized according to an observation of knife cleaning behavior in a kitchen. Adenosine triphosphate (ATP) bioluminescence was …

Pharmaceuticals And Personal Care Products: Emerging Contaminants In Aquatic Ecosystems, Jenny E. Zenobio

Open Access Theses

In recent years, the presence of pharmaceuticals and personal care products (PPCPs) in aquatic systems has led to research on their fate and effects. PPCPs have been found in mixture in wastewater effluents, surface, ground, and drinking water at low concentrations from areas of intense urbanization. Although adverse effects to human health from the current environmental concentrations are unlikely, the impacts to ecological receptors are not clear. We performed field and laboratory studies to quantify and evaluate effects of PPCPs on fish. First, a field study was conducted at the Baca National Wildlife Refuge, Colorado (2010-2012) because a portion of …