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Articles 31 - 60 of 1261
Full-Text Articles in Life Sciences
Influence Of Soil And Land Cover On Chronic Wasting Disease Prevalence In White-Tailed Deer And Mule Deer Across North America, Madison L. Miller
Influence Of Soil And Land Cover On Chronic Wasting Disease Prevalence In White-Tailed Deer And Mule Deer Across North America, Madison L. Miller
Masters Theses
Chronic wasting disease (CWD) is a fatal neurodegenerative disease that infects deer and is caused by a pathogenic prion. CWD is a concerning wildlife disease because it is incurable, potentially poses a risk to human health, and is spreading rapidly. CWD prions are transmitted both directly via bodily fluids and indirectly through environmental reservoirs such as soil. In this study, we investigated the influence of land cover and soil characteristics on CWD prevalence in white-tailed deer and mule deer. We acquired CWD prevalence data from seven North American regions and used ArcGIS to obtain land cover and soil characteristic data …
Research Day 2022 Program, Western Michigan University Homer Stryker M.D. School Of Medicine
Research Day 2022 Program, Western Michigan University Homer Stryker M.D. School Of Medicine
Research Day
No abstract provided.
An Investigation Of Chronic Pain As A Much-Neglected Symptom Of Hypermobile Ehlers-Danlos Syndrome, Sydney Collins
An Investigation Of Chronic Pain As A Much-Neglected Symptom Of Hypermobile Ehlers-Danlos Syndrome, Sydney Collins
Honors Theses
Ehlers Danlos Syndrome is a group of heritable loose connective tissue disorders with 13 distinguished subtypes. The hypermobile type of Ehlers Danlos Syndrome (hEDS) is the most common subtype and is caused by a genetic mutation that leads to defective collagen fibrils. This leads to joint instability and hypermobility, skin elasticity, widespread pain, fatigue, and generalized tissue fragility. Chronic pain is reported to be a symptom in as high as 92% of the hEDS population (Voermans et al., 2010); despite this prevalence, there is a significant lack of research, awareness, and treatment standardization regarding pain in hEDS. This literature review …
The Flavoprotein Rcla Is A Hypothiocyanous Acid Reductase, Irina Chapman
The Flavoprotein Rcla Is A Hypothiocyanous Acid Reductase, Irina Chapman
Masters Theses
Reactive chlorine species (RCS) - hypochlorous acid (HOCl), hypothiocyanous acid (HOSCN), and chloramines - are powerful antimicrobial oxidants generated by the innate immune system. Bacteria developed defense mechanisms against RCS, which are highly toxic for invading pathogens. One such defense system involves RclA, an enzyme from the flavin disulfide reductase family, which is massively upregulated upon exposure of Escherichia coli to RCS. Recent research suggests that RclA functions as a Cu(II) reductase to provide resistance against RCS. However, the exact mechanism used by RclA was subject to debate. In this study, we demonstrate that RclA is unlikely to function as …
The Impacts Of Aging, Sedentarism, And Exercise On Neurotrophic Factor Expression And Innervation In The Heart And The Effects Of Treatment With Α-Cgrp On Heart Function, Gabriel Almeida Alves
The Impacts Of Aging, Sedentarism, And Exercise On Neurotrophic Factor Expression And Innervation In The Heart And The Effects Of Treatment With Α-Cgrp On Heart Function, Gabriel Almeida Alves
Dissertations
Neurotrophic factors (NFs) are important molecules responsible for development, differentiation, regeneration, and maintenance of new and mature neurons. Neurotrophic factors act as neurocytokines and may assist with the regulation of axonal and dendritic arrangements and synaptic plasticity between neurons themselves or with other non-neural target tissues. In this study, we analyze the levels of two NFs: glial cell line-derived neurotrophic factor (GDNF) and nerve growth factor (NGF). Cardiomyocytes produce these neurotrophic factors which assist with the innervation pattern of the heart. The heart is innervated by the two branches of the autonomic nervous system; namely the sympathetic nervous system and …
Environmental Justice Analysis Of Drinking Water Policies In Michigan Cities, Annika Paldan
Environmental Justice Analysis Of Drinking Water Policies In Michigan Cities, Annika Paldan
Honors Theses
This thesis examined environmental justice aspects of drinking water policies in four cities across Michigan: Flint, Birmingham, St. Joseph, and Benton Harbor. An overview of the history of the environmental justice movement, environmental racism, and drinking water policies provided the basis for a four-part evaluative criterion to assess environmental justice components at the municipal level. These criteria include (1) housing tenure, (2) age, size, and service line composition of the infrastructure, (3) public participation in the policy process, and (4) emergency management of the city. Findings indicate that environmental justice has come a long way, with cities now incorporating components …
Literature Review Of Human Hdr Syndrome With Gata3 Haplo Insufficiency, Olivia Ambursley-Gries
Literature Review Of Human Hdr Syndrome With Gata3 Haplo Insufficiency, Olivia Ambursley-Gries
Honors Theses
This literature mining project is regarding mutations that cause hearing loss in patients with human hypo parathyroid, deafness, and renal dysplasia (HDR) syndrome caused by a mutation in the GATA3 gene. The disease is unique; not all diagnosed individuals present with all symptoms of the triad. Van Esh et al. found that the haploinsufficiency of the GATA3 is found on chromosome 10p14-p15 (ref.1). The focus of this work is to condense the literature on GATA3 haploinsufficiency correlating to human HDR syndrome. Due to the lack of global healthcare access, it can be assumed that many cases remain undiagnosed; 180 have …
Retinal Regeneration In A Genetic Mouse Model Of Glaucoma, Joshua Paris
Retinal Regeneration In A Genetic Mouse Model Of Glaucoma, Joshua Paris
Masters Theses
Glaucoma is a degenerative retinal disease characterized by progressive loss of retinal ganglion cells (RGCs). Previous studies have shown that application of a specific α7 nicotinic acetylcholine receptor agonist, PNU-282987 (PNU), onto the murine retina induces neurogenesis of numerous retinal cell types, including RGCs. The aim of this study is to characterize the short-term and long-term effects of PNU in a glaucoma model. The effects of PNU were analyzed in a DBA/2J mouse model that auto-induces a glaucoma-like condition in adulthood. These mice manifest an elevated intraocular pressure (IOP) starting at 6 months, followed by loss of RGCs. To assess …
Effects Of Traditional And Microbially-Focused Restoration Techniques On Soil Communities In Tallgrass Prairies, Zachary J. Whitacre
Effects Of Traditional And Microbially-Focused Restoration Techniques On Soil Communities In Tallgrass Prairies, Zachary J. Whitacre
Masters Theses
Tallgrass prairies have virtually disappeared in many parts of their former range due to the conversion of this ecosystem to farmland. In more recent years there have been efforts to restore these prairies on reclaimed agricultural land. However, these restored prairies do not resemble their remnant counterparts in many ways, such as in soil microbial community composition and metrics related to carbon storage. In Chapter 1, I show that bacterial communities in a restored prairie and an adjacent remnant prairie in southwest Michigan differ in their immediate and longer-term responses to prescribed fire, a commonly used prairie restoration and maintenance …
Strong Women Breaking Ground: Roles Of Women In Agriculture In Michigan, April L. Shirey
Strong Women Breaking Ground: Roles Of Women In Agriculture In Michigan, April L. Shirey
Masters Theses
Agriculture in Michigan is changing. While the number of farms and farmers continue to decrease, women are increasingly taking on the role of farmer instead of the “farmer’s wife”. The number of female producers increased from 8,275 to 26,059 where the number of producers in Michigan decreased from 56,014 to 47,641 from 2007 to 2017 (USDA, 2007, 2017). Women are becoming the face of farming in Michigan, yet little research examines the impacts of these shifts. In this research, I conduct semi-structured interviews with female farmers throughout lower Michigan beginning in the summer of 2020 to learn more about these …
Evaluation Of Α-Pyrrolidindovalerophenone (Α-Pvp) And Its Isomers In Male And Female Sprague-Dawley Rats Trained To Discriminate Mdpv, Kaley Cargile
Evaluation Of Α-Pyrrolidindovalerophenone (Α-Pvp) And Its Isomers In Male And Female Sprague-Dawley Rats Trained To Discriminate Mdpv, Kaley Cargile
Masters Theses
Recreational use of synthetic cathinones, 3,4-methylenedioxypyrovalerone (MDPV) and α‐pyrrolidinovalerophenone (α‐PVP), has become increasingly popular, thus prompting characterization of their behavioral and neurochemical effects. MDPV has been studied for several years now, though there is still much unknown about α‐PVP, and its isomers. The primary objective of this study was to characterize the discriminative effects of α‐PVP and its isomers in comparison to MDPV. Male and female Sprague-Dawley rats were trained to discriminate 0.5mg/kg MDPV from saline injections under a fixed ratio 20 (FR 20) schedule of food reinforcement. Substitution tests were conducted with MDPV (0.05-0.5mg/kg), α‐PVP (0.05-0.5mg/kg), (S)-α‐PVP (0.05-1mg/kg) and …
Effects Of High Intensity Interval Training On Cardiorespiratory Fitness And Body Composition In Women: A Review, Rachel Marie Dykstra
Effects Of High Intensity Interval Training On Cardiorespiratory Fitness And Body Composition In Women: A Review, Rachel Marie Dykstra
Dissertations
Background: A growing body of literature indicates that women, in comparison to men, report significantly more barriers to exercise, often times related to perceived lack of time due to their social roles (e.g. family responsibility, childcare). Therefore, it has been considered that exercise programs that require less time commitments would be more attractive for women to alleviate this concern. Objective: This review was conducted to comprehensively understand the present literature surrounding women and such a time-efficient protocol: high intensity interval training (HIIT). The primary aim was to determine the impact of HIIT on both cardiorespiratory fitness (CRF) and body composition. …
How Gata3 Affects Neuronal Survival In The Inner Ear, Hiba Ahmad
How Gata3 Affects Neuronal Survival In The Inner Ear, Hiba Ahmad
Honors Theses
Broadly speaking, Gata3 is a transcription factor involved in neurosensory specification and hair cell differentiation. Previous studies have shown that Gata3 null mutants do not develop neurons, so Gata3 appears to be necessary for neuronal survival. To better understand the role of Gata3 in spiral ganglion neurons of the inner ear, a Neurod1-cre model was used to conditionally knock out Gata3 at E9-E9.5, the timepoint when neuroblasts first begin to delaminate from the otic placode. The mutants used in this study were Nd1-cre: Gata3 f/f mutants. In situ was performed to determine gene expression within the cochlea, and immunohistochemistry was …
Crayfish And Climate Change: How The Growing Acidity Of Freshwater Lakes, Streams, And Ponds Negatively Impact The Nervous System Of Crayfish, Lauren Irmen
Honors Theses
The nervous system of crayfish, a freshwater crustacean, is largely regulated by the flow of calcium (Ca2+) ions in a cell across a concentration gradient that produces electrical impulses and controls movement (Krause et al., 1998). Crayfish obtain Ca2+ from their external aquatic environment. Therefore, rapid changes in the concentration of Ca2+ in freshwater environments may cause negative impacts to the neural system of crayfish and subsequently the freshwater food chain. These impact of altered Ca2+ levels may create hyperactive or hypoactive movement due to over-, under-stimulation, respectfully in the skeletal muscle of the crayfish, …
Regenerative Rehabilitation And Genomics: Implications For Occupational Therapy, John V. Rider
Regenerative Rehabilitation And Genomics: Implications For Occupational Therapy, John V. Rider
The Open Journal of Occupational Therapy
The completion of the human genome project has paved the way for health care practitioners to use genetic and environmental information to tailor medical treatment. This innovative approach to health care is rapidly evolving, and occupational therapists need to be aware of the impact it will have on future practice. Regenerative rehabilitation is a product of knowledge and techniques from the fields of rehabilitation and regenerative medicine with the common goal to restore function. Occupational therapists have the potential to play a significant role in regenerative rehabilitation research and implementation. The purpose of this article is to (a) increase understanding …
Patella Resection In Total Knee Arthroplasty: An Analytical Comparison Of Three Techniques, Kelsey Cushway
Patella Resection In Total Knee Arthroplasty: An Analytical Comparison Of Three Techniques, Kelsey Cushway
Research Day
Patella resection, as a routine component of TKA, can be both difficult to plan and difficult to execute. The primary purpose of this study was to evaluate the repeatability of three unique patellar resection techniques used in total knee arthroplasty. The secondary purpose of this study was to establish whether different surgical techniques were able to reproduce preoperative plans made by each surgeon. We used radiographic measurements to evaluate patellar thickness and patellar cut angle preoperatively and postoperatively. Three techniques (45 cases in total) were evaluated, revealing qualitative differences between surgical techniques and significant quantitative differences between average patellar thickness …
Research Day 2021 Program, Western Michigan University Homer Stryker M.D. School Of Medicine
Research Day 2021 Program, Western Michigan University Homer Stryker M.D. School Of Medicine
Research Day
No abstract provided.
Comparison Of Cardiac Muscle Between Male And Female Rats With Exercise And Aging: Analyzing Vitals, Gdnf Expression, Ngf Expression, Rna Expression, Sympathetic Innervation, Parasympathetic Innervation, And Sensory Innervation, Katelyn Kurrie
Honors Theses
The direction of my research-based thesis answers the question of how exercise affects the cardiovascular system with aging in male and female rats. My study would highlight the important differences of the cardiovascular system of both sexes which could lead to sex-specific insight on the cardiovascular disease epidemic which prevails as the most common cause of death in the United States. The studies completed will test the hypothesis: neurotrophic factor will be higher in females than males due to the higher presence of estrogen in females. The trophic factor is predicted to increase in both males and females with regular …
An Examination Of Barriers And Determinates Impacting The Healthcare Of Kalamazoo County's Transgender And Non-Binary Young Adult Population, Tyler Signorella
An Examination Of Barriers And Determinates Impacting The Healthcare Of Kalamazoo County's Transgender And Non-Binary Young Adult Population, Tyler Signorella
Honors Theses
Historically non-binary and transgender individuals have faced and continue to face a unique set of obstacles and challenges in seeking and receiving general healthcare. Within the US this is well documented on a national level. In this exploratory study we will seek to understand the experience of young adults in Kalamazoo County comparing their perspectives to the already established pool of information. This will be accomplished through surveying emergent adults identifying as either transgender or gender non-binary within Kalamazoo County and then synthesizing this data into an analyzable collective format.
Incorporating Worksheets To Enhance Active Learning In A Laboratory Course, Zakiya S. Kelley
Incorporating Worksheets To Enhance Active Learning In A Laboratory Course, Zakiya S. Kelley
STEM Instructional Graduate Teaching Assistant Posters
Study Goals:
To create worksheets that would aid the students in properly completing the required experiments and challenge them to do more than just follow the instructions. Each week there were often multiple experiments to be completed and pre-made samples to be observed within the class period. By providing the worksheet, I ensured the students completed all tasks and comprehended the covered material before class ended.
Objectives:
- To improve student participation and information retention when completing laboratory experiments in the classroom
- To emphasize key points of the topic being explored
The Role Of Homologous Cyclin B’S In The Cell Cycle And, In Their Absence, The Effect On Zebrafish Early Development, Tetiana Petrachkova
The Role Of Homologous Cyclin B’S In The Cell Cycle And, In Their Absence, The Effect On Zebrafish Early Development, Tetiana Petrachkova
Dissertations
Regulation of cell division is essential for normal embryo development. The Cyclins and their Cyclin-dependent kinases are key regulators controlling this process. In this thesis, I examine the role of cyclin B1 and cyclin B2 in zebrafish development. It is thought that both Cyclins are necessary for a cell to progress past the G2/M checkpoint into mitosis. First, I show that zygotic Cyclin B1 is essential for normal cell cycle progression, but not for cells to enter mitosis. Lack of zygotic Cyclin B1 in the early arrest mutant specter, which carries a nonsense mutation in the cyclin B1 gene, …
The Assessment Of Co2 Exposures, Workload Demands, And Musculoskeletal Disorder Risk On Heart Rate For Select Craft Brewery Job Tasks, David L. Huizen
The Assessment Of Co2 Exposures, Workload Demands, And Musculoskeletal Disorder Risk On Heart Rate For Select Craft Brewery Job Tasks, David L. Huizen
Dissertations
Americans have embraced craft beer, and the ensuing demand has resulted in an ever-increasing number of craft breweries opening over the past 20 years. Craft brewers are generally small employers and, as such, lack the resources necessary to address the safety and health of their employees adequately. Brewers face many safety and health hazards in their work environment. These hazards include exposures to carbon dioxide (CO2), workload demand, and MSD risk. It is essential to evaluate these hazards in craft breweries as they handle carbon dioxide (CO2) differently than large breweries by having the CO2 …
Changes In Gene Expression Profiles In Müller Glia Following Exposure To An Α7 Nicotinic Acetylcholine Receptor Agonist, Megan L. Stanchfield
Changes In Gene Expression Profiles In Müller Glia Following Exposure To An Α7 Nicotinic Acetylcholine Receptor Agonist, Megan L. Stanchfield
Masters Theses
Previous studies from this lab have determined that dedifferentiation of Müller glia (MG) occurs after application of an α7 nicotinic acetylcholine receptor agonist, PNU-282987 (PNU), to retinal pigment epithelial (RPE) cells in adult rodents. This study was designed to explore the role of the HB-EGF/Ascl1/Lin28a signaling pathway in MG dedifferentiation to retinal progenitor cells. RNAseq was performed on MG following contact with RPE-J cells treated with PNU-282987. Up- or down-regulated genes were compared with published literature of MG dedifferentiation that occurs in lower vertebrate regeneration or with transcript profiles during early mammalian development. Between 8-12 hours, up-regulation was observed in …
High-Efficiency Transformation Of Yeast By Electroporation, Lilly Ruell
High-Efficiency Transformation Of Yeast By Electroporation, Lilly Ruell
Honors Theses
This paper analyzes and optimizes efficiency in the yeast transformation protocol using electroporation. Three variables were tested for optimization, they are the following: DNA concentration, cell density and electroporation voltage. Further verification of transformation was done through the analyzation of HPLC data to ensure protein production. The results conclude that DNA concentration was the most important dependent variable for transformation efficiency. A voltage of 2000V is the optimal condition to achieve efficient yeast transformation. Cell density had no observational difference in transformation efficiency at the range of DNA concentration necessary for research use. Improving this efficiency of the yeast transformations …
Putative Flagellar Channel-Lining Residues And Their Role In Filament Synthesis, Morphology And Stability, Troy Burtchett
Putative Flagellar Channel-Lining Residues And Their Role In Filament Synthesis, Morphology And Stability, Troy Burtchett
Masters Theses
Bacterial flagella are comprised of ~20,000 repeating subunits called flagellin and are synthesized by transporting unfolded flagellin monomers through the central channel of the flagellar filament to the distal end where they are folded and incorporated into the growing structure. The transport mechanism is predicted to be facilitated by a hydrophilic coating of amino acid side chains on the inside wall of the central channel that repels the mostly hydrophobic surface of the unfolded flagellin monomer. The lack of interaction between the flagellin monomer and the central channel wall is thought to allow for the passive diffusion of protein monomers. …
Organized Caudal Photoreceptors In The Medicinal Leech, Baylee Bancroft
Organized Caudal Photoreceptors In The Medicinal Leech, Baylee Bancroft
Honors Theses
Visual systems are essential to an organism’s survival. There are a wide range of photosensory organs across the animal kingdom, varying by means of structure, complexity, and the way in which they transduce information. Regardless of variation, visual systems provide the organism with information regarding external stimuli based on how light interacts with surrounding matter. The medicinal leech, Hirudo verbana, is one of the many important model organisms of neurobiology as this segmented annelid possesses a relatively simple nervous system and a rudimentary visual system. The leech also exhibits a restricted range of quantifiable overt behaviors and is capable of …
Examining The Effects Of Time On Antibiotic Resistance Within Prairie Restorations, Haley Ritsema
Examining The Effects Of Time On Antibiotic Resistance Within Prairie Restorations, Haley Ritsema
Honors Theses
Widespread antibiotic use in agriculture spreads antibiotic resistance (AR) genes throughout the ecosystem and can impact bacterial community composition and function. Restoring agricultural fields back to their native prairies has been shown to remediate other damaging effects of agriculture, and have the potential to remediate AR from the soil as well. In this study, our first aim was to determine if soil bacterial communities return to pre-agricultural conditions over time following restoration to native plant communities. We did this by assessing the soil characteristics, extracellular enzyme activities, bacterial community composition, and functional metagenomics across a chronosequence of seven restored prairies, …
The Effect Of Pre-Workout Supplementation On Fitness Assessment Tests, Kassidy Davis
The Effect Of Pre-Workout Supplementation On Fitness Assessment Tests, Kassidy Davis
Honors Theses
Research regarding the benefits and detriments of Pre-workout supplement has gradually increased since its popularity arose in the early 1980’s. There have been a number of studies on its effects on human physiology during a workout; however, there are few studies regarding Preworkout supplement during fitness assessment tests. Therefore, further insights concerning Preworkout supplement and fitness testing is needed. Pre-workout supplement is taken prior to a training session and has been shown to enhance a workout by increasing aspects such as focus, reaction time, stamina, and power. This could be viewed as solely a psychological enhancement than an actual physical …
Glut5 And Fructose Metabolism Inhibition In A Simulated Small Intestine Environment, Isabella Trainor
Glut5 And Fructose Metabolism Inhibition In A Simulated Small Intestine Environment, Isabella Trainor
Honors Theses
This thesis describes an experiment that was designed in order to obtain information that could be useful for treating individuals with HFI. The author hypothesized that MSNBA would inhibit GLUT5 and fructose metabolism in the small intestine. The final experimental design involves exposing a small intestine cell line to fructose and MSNBA. The cell line would be exposed to MSNBA over the course of three different periods, and data was intended to be obtained using western blotting and a fructose assay kit. Although the final experimental design project was not fully carried out due to circumstances surrounding COVID-19, a plausible …
Understanding Stem Instructors’ Experiences With And Perceptions Of Deaf And Hard Of Hearing Students: The First Step Towards Increasing Access And Inclusivity, Makayla Long
Honors Theses
Through an anonymous survey we analyzed Western Michigan University (WMU) STEM instructors’ experience with and understanding of teaching and interacting with Deaf and Hard of Hearing (HoH) students. The goal of this study was to capture the experiences of WMU STEM instructors and the resources available to them given their direct impact on Deaf/HoH students’ ability to succeed and receive a post-secondary education. The findings of this study support the claim that instructors are not well supported or aware of the needs of Deaf/HoH students, and do not have access to adequate resources to resolve the issue.
The first steps …