Life Sciences Commons^™

Consistency Is Key: Investigating Vocal Consistency In Field Sparrows, Stephanie Stanton

Masters Theses

No abstract provided.

Exploring Song As A Reproductive Isolation Mechanism In A Hybridizing Population Of Winged Warblers, Amy E. Janik

Masters Theses

Reproductive isolation mechanisms such as song are used by birds to promote assortative mating by species and prevent gene flow when closely related species come into secondary contact. Based on the strength of a reproductive barrier, signals in hybrid zones can diverge and become more dissimilar to maintain species or converge to become more similar and make species discrimination more difficult. When two closely related species overlap in territories and have similar songs, song convergence is likely and has the capacity to disrupt species recognition. We test the hypothesis that song acts as a reproductive isolation mechanism by examining song …

Physiology And Genetics Shape The Microbiome Of A Seabird Species (Oceanodroma Leucorhoa) More Than Environmental And Social Factors, Douglas Steven Pearce

Masters Theses

The microbiome provides multiple benefits to animal hosts that can profoundly impact health and behavior. Microbiomes are well-characterized in humans and animals in controlled settings, yet assessments of wild bird microbial communities remain vastly understudied. This study examines the multiple factors that affect the microbiome of a burrow-nesting Procellariiform bird species, Leach’s storm-petrel. 16S rRNA-based Illumina Mi-Seq analyses are used to assess the composition and structure of bird and burrow-associated bacterial communities. Results indicate that sex and skin site contribute to bird-associated bacterial community variation, and MHC heterozygosity impacts these bacterial assemblages in a sex and site-specific manner, potentially having …

The Application Of Chitosan On An Experimental Infection Of Pseudogymnoascus Destructans Increases Survival In Little Brown Bats, Torin C. Kulhanek

Masters Theses

This research builds upon a previous study conducted that tested the efficacy of chitosan on experimentally-infected bats as a laboratory model of White-nose syndrome. White-nose syndrome is a fungal infection caused by Pseudogymnoascus destructans, formerly known as Geomyces destructans. The pathology of this disease is not yet fully understood, but it is devastating bats throughout Northeastern America and continues to spread. Here we tested multiple application time points and concentrations of chitosan on the wings of experimentally-infected bats and compared them with the controls. We grossly viewed the wings first to get a sense of the amount of …

Injury-Induced Neuronal Turnover With Zinc Sulfate Affects Cilitated Olfactory Sensory Neurons More Than Microvillous Olfactory Sensory Neurons In The Adult Zebrafish, James Hentig

Honors Theses

Zinc sulfate is a known olfactory toxicant, although its effects on the olfactory epithelium of zebrafish have not been examined. Previous observations utilizing Triton X-100 found selective retention of some olfactory sensory neurons and loss of others. Based on those findings, we hypothesized that the retention of the ability to sense amino acids, detected by microvillous olfactory sensory neurons, and loss of the ability to perceive bile salts, detected by ciliated olfactory sensory neurons, is a universal response to chemical ablation. Fish were treated with zinc sulfate and survived for 2, 5, 10 and 14 days post treatment and processed …

One Fish, Two Fish, Red Fish, Blue Fish: The Analysis Of The Specter Cell Cycle Mutant In Zebrafish, Tetiana Petrachkova

Masters Theses

Cell division is controlled by genes that regulate the cell cycle. Here we show that the zebrafish specter (spr) mutant is mutation in the cyclin B1 gene, a gene necessary for the G2 to M transition of the cell cycle.

The spr mutation mapped to the cyclin B1 gene. Sequencing showed a transition (C139→T) that caused a nonsense mutation in exon 2 of the cyclin B1 gene. In situ hybridization of cyclin B1 revealed that the mRNA is absent in the mutant embryo by gastrulation. We found that the earliest visible mutant phenotype was a darkening of the head caused …

Extrinsic And Intrinsic Factors Influence Parasite Burden In The Big Brown Bat (Eptesicus Fuscus), Maarten Vonhof

Faculty Research and Creative Activities Award (FRACAA)

In most host-pathogen systems, the key feature of transmission dynamics and rate of spread of a parasite or disease is the variation among hosts in the numbers of parasites they harbor and the factors controlling infection. Pathogens are typically aggregated across their host populations: relatively few individuals are heavily infected, while most potential hosts suffer light infections or remain uninfected. Aggregation among hosts may be generated by variation among individuals in their exposure to parasite infective stages and by variation in susceptibility once an infectious agent has been encountered. Host heterogeneity in parasite burden has a strong influence on the …

Microglia Respond To Both Permanent And Temporary Deaferentation In The Adult Zebrafish Olfactory Bulb, Amanda K. Mckenna

Masters Theses

The major goal of this study was to investigate a microglial response following deafferentation in the adult zebrafish, Danio rerio, olfactory bulb. Microglia were identified in the olfactory bulb using the 4c4 antibody and phagocytic-dependent labeling (DiA). Microglia responded to both methods of deafferentation namely, permanent (cautery) and temporary (Triton X- 100). On one and three days following both permanent and temporary deafferentation there was a significant increase in microglia in the treated olfactory bulb. At five and seven days after permanent and temporary deafferentation there was no longer a significant difference between the treated and untreated olfactory bulbs, …

Salmonid Habitat Restoration On The Chocolay River, Michigan, Ross J. Crawford

Masters Theses

This project seeks to improve salmonid habitat quality by improving riparian vegetation on the adjacent banks (from toe to terrace) on the Chocolay River in Michigan's Upper Peninsula. Quantities of large woody debris (LWD) were also analyzed to determine the heterogeneityof stream habitats, or channel roughness. Percentages of rock, gravel, sand, and silt were analyzed to determine spawning habitat quality. As the proportions of fines (

Population Demographics And Genetics Of Spix's Disk-Winged Bat: Insights Regarding Survival, Mate Choice, Gene Flow And Effective Population Size, Michael Buchalski

Dissertations

Simultaneous study of the demographics and genetics of populations are relatively rare within the literature, despite insights their combined use offers regarding the life history, ecology, and evolution of species. Here I take a comprehensive approach, using capture-recapture data, polymorphic microsatellite markers, and various modeling techniques to examine the demographics and genetics of multiple local populations of Spix’s disk-winged bat (Thyroptera tricolor) in southwestern Costa Rica. T. tricolor is a highly gregarious, neotropical bat species known to form kin-based social groups with high retention of offspring of both sexes. The implications of this highly unusual social structure for …

The Rise And Fall Of Kin Structure In The Neotropical, Family-Living Buff-Breasted Wren (Cantorchilus Leucotis) In Gamboa, Panama, Sarah C. Alessi

Masters Theses

Family-living species provide an exciting model to examine how natal dispersal and kin structure influence genetic structuring within local populations. Juvenile buff-breasted wrens (Cantorchilus leucotis) of both sexes delay dispersal and exhibit short-distance natal dispersal, which should lead to kin-structured populations in which relatives of both sexes occupy neighboring territories. Blood samples collected from juvenile and adult wrens in Gamboa, Panama were analyzed using microsatellite markers to determine whether related individuals are spatially clustered on neighboring territories, spatial clusters of relatives change over time, and if kin structure is sex-specific. Global and local spatial autocorrelation analyses detected genetic …

Characterizing Potential Sustainable Energy Feedstocks, Jesse George Thompson

Dissertations

Trap grease offers a promising and less expensive alternative to food grade vegetable oils for biodiesel production, but its commercial marketability could be affected by its intense and offensive odor. Identification, quantitation and eventual elimination of the odorant compounds are vital challenges that need to be addressed before commercialization can be achieved. A rapid method using HS-SPME and GC/MS was developed in this study to identify and quantify the major volatile odorant compounds present in trap grease. The main odorant compounds were identified as short chain free fatty acids (SC-FFA) from C₄ - C₁₀.

Sensory evaluations by …

Characterization Of Membrane Potentials In Vascular Smooth Muscle Of Hagfish, Lamprey And Trout, Prentiss Jones Jr.

Dissertations

The objective of this study was to characterize membrane potentials in systemic arteries of Pacific hagfish, Eptatretus stouti, Rainbow trout, Onchorhyncus mykiss, and Sea lamprey Petromyzon marinus. Previous studies have characterized membrane potentials in piscine tissue. However, these studies utilized non-vascular tissues such as cardiac muscle and skeletal muscle (69, 161). Characterization of membrane potentials in fish vasculature is without precedent in the literature. The hypothesis of this study was that membrane potentials in fish vascular smooth muscle differ little between species when comparable vasculature is evaluated.

Comparative Genomic Analyses And Expression Of A Set Of Overlapping Open Reading Frames Of Frog Virus 3, Type Species Of Ranavirus (Family Iridoviridae), Wendy Guat Hoon Tan

Dissertations

Frog virus 3 (FV3) is the type species member of the genus Ranavirus (family Iridoviridae ). To better understand the molecular mechanisms involved in the replication of FV3, including transcription of its highly methylated DNA genome, the complete nucleotide sequence of the FV3 genome has been determined. The FV3 genome is 105, 903 base pairs long excluding the terminal redundancy. The G+C content of FV3 genome is 55% and it encodes 98 non-overlappingpotential open reading frames (ORFs) containing 50 to 1293 amino acids. Eighty-four ORFs have significant homology to known proteins of other iridoviruses, whereas 14 are unique FV3 ORFs. …

Bufadienolides In The Chemical Defenses Of The Toads, Bufo Americanus And Bufo Fowleri, Catherine E. Merovich

Dissertations

Resource equalization represents the historical and theoretical basis for American school finance and its ideals continue to dominate discussion and practice. During the last decade and a half, the national aspiration for equitable distribution of school finance resources has started a transformation into a derivative notion of adequacy. This equity policy derivative embraces the idea of sufficiency, which in turn implies a standard. The concept of educational adequacy has grown to mean the achievement of high absolute standards. Equity (equalization) theorists measure resource level on a relative basis; each student relative to all others. Adequacy adherents measure student achievement against …

Health Impacts Of Polychlorinated Biphenyls (Pcbs) In Aquatic Organisms, Marla Ann Fisher

Dissertations

Polychlorinated biphenyls (PCBs) are a major contaminant of global extent in water resources and aquatic biota. Due to its high lipid solubility, PCBs fail to be degraded and, therefore, continue to bioaccumulate throughout the environment and food chain. To determine the impact of PCBs on the immune system ofaged and juvenile Japanese medaka (Oryzias latipes), fish were injected with the coplanar PCB congener 126 and examined after 3 and 14 days. PCB 126 produced oxidative stress in both age groups of fish 14 days post-injection; however, juvenile medaka appeared more susceptible than aged fish. Humoral immunity, as determined by antibody …

Identification And Classification Of Coryneform Bacteria Isolated From Bovine Mammary Glands, Jeffrey L. Watts

Dissertations

Coryneform bacteria are frequently isolated from bovine mastitis and these infections are associated with economic losses. Corynehacterium bovis, a lipid requiring species, has been the most frequently isolated coryneform from the milk of infected bovine mammary glands. However, the taxonomic status of this organism is uncertain. In the current study, a polyphasic approach was used to identify coryneform bacteria isolated from bovine mastitis and determ ine the phylogenetic relationships among the identified species. A total of 212 coryneform bacteria isolated from bovine mastitis was obtained from mastitis reference laboratories in the United States and Canada. Presumptive identification based upon …

The Effects Of Photoperiod On The Prolactin Cells Of Notropis Cornutus (Mitchell), Matthew Kevin Hettinger

Masters Theses

Three groups of the common shiner (Notropis cornutus, Mitchell) were adapted to three different photoperiods. These photoperiods were 16L:8D (summer), 12L:12D (controls), and 8L:16D (winter). The prolactin cells were studied histologically and morphologically with respect to staining characteristics, cell and nuclear morphology, nuclear size, and cellular density. The prolactin cells of those fish under the long photoperiod exhibited greater cellular activity than the controls as evidenced by an increase in nuclear size, cellular density, and by becoming more circular. The prolactin cells of those fish under the short photoperiod exhibited the same characteristics as those in the long photoperiod. The …

Studies On The Scorpions Of Libya, Nuri Milad Barbash

Masters Theses

No abstract provided.

Effects Of Indomethacin On Gastric Secretion And Duodenal Ulcer Formation In Bile Duct-Ligated Rats, Diane M. Przewozniak

Masters Theses

No abstract provided.

Effect Of Cold Exposure On Serum Levels Of Corticosteroid-Binding Globulin In Male And Virgin Female Rats, Erik R. Larsen

Masters Theses

No abstract provided.

Studies Of Michigan Lycosidae, Robert John Wolff

Masters Theses

No abstract provided.

Physiological Iron Deficiency Anemia In Suckling Beagle Pups, Timothy F. Porter

Masters Theses

No abstract provided.

Territory And Mating System In The Bobolink, Dolichonyx Oryzivorus, Arlo J. Raim

Masters Theses

No abstract provided.

Limnodrilus Hoffmeisteri: Observations Of The Coelomic Cells And Stage I Sperm Under Laboratory Conditions, Field Conditions, And After Exposure To Several Levels Of Radiation, Edward Michael Block

Masters Theses

No abstract provided.

A Comparative Study Of The Helminth Fauna Of Salmo Gairdneri Rich. From Three Different Environments: A Hatchery, A Lake And, A River, Jose Felipe Ribeiro Amato

Masters Theses

No abstract provided.

Organization Of The Bird Community Of A Michigan Oak Forest, Jerome D. Wenger

Masters Theses

No abstract provided.

Populations Of Some Mammals Of Oak Forests In Southwestern Michigan, James R. Hodgson

Masters Theses

No abstract provided.

Bird Populations Of Three Oak Forest Areas In Southern Michigan, Ted B. Gottshall

Masters Theses

No abstract provided.

The Accumulation And Retention Of Radiophosphorous By Escherichia Coli, Douglas Gunnison

Masters Theses

No abstract provided.