Biomedical Engineering and Bioengineering Commons^™

Design, Development, And Characterization Of Breathforce : A Respiratory Training System For Patients With Spinal Cord Injuries., Kevin L Tran

Electronic Theses and Dissertations

Pulmonary and cardiovascular dysfunction are consistently reported as the leading causes of morbidity and mortality among the 1,275,000 people who are living with chronic spinal cord injury (SCI) in the United States. Respiratory-cardiovascular complications from neurological disorders (primarily COPD and sleep apnea) are currently the number one cause of death and disability in the US.

The main goal of this project is to develop an inspiratory-expiratory training device for use in the rehabilitation of patients with respiratory motor and cardiovascular deficits that incorporates existing technologies and promotes successful training methodologies performed at the clinic and at home.

An embedded microprocessor …

Biomechanical Testing Of An Exercise For Strengthening The Proximal Femur., Alyssa Osbourne

Electronic Theses and Dissertations

Based on the principles of cutting edge bone remodeling research, a unique therapeutic exercise device was designed specifically to improve bone quality at the most critical location of the proximal femur prone to fracture: the superior-lateral femoral neck where the fracture first initiates during a fall. The exercise/device is intended to work by inducing enough strain in the bone to stimulate the body’s natural bone remodeling mechanisms to increase bone density in the proximal femur and consequently prevent a fracture from arising if a fall to the side does occur.

In order to test the proposed exercise, experiments simulating the …

Custom Software For The 3d Printing Of Patient Specific Plate Bending Templates In Pelvic Fracture Repair., Gordon B Lents

Electronic Theses and Dissertations

The purpose of this work is to reduce the operative time and blood loss incurred during open reduction and internal fixation (ORIF) of traumatic pelvic injuries through the creation of patient specific bending templates for reconstruction plates. These templates are 3D printed in a resin capable of being sterilized and taken into the operating room so that bending may be performed by the surgeon before the patient is opened or by another team member in parallel with the surgeon.

A novel software extension was created in 3D modeling software to allow a surgeon to individually position screws on a pelvic …

Development And Evaluation Of A Biocompatible Electroactive Sensor For Continuous Blood Pressure Measurement., Scott D. Cambron

Electronic Theses and Dissertations

Piezo-active composites have been implemented for sensing and transduction for decades. The 0-3 ceramic/polymer composite is one of the most common composite types used for sensing applications, owing to their tailorable properties of the two-phase composition, consisting of a three-dimensionally connected polymer/rubber matrix (inactive phase) with a dispersion of isolated piezo-ceramic particles (active phase). This thesis describes a method to develop novel biocompatible perivascular band comprised of a two-phase piezo-active composite to be fabricated using simple manufacturing processes. Biomaterials such as tissue scaffolds comprised of silk fibroin (SF) and chitosan (CS), and biocompatible soft rubbers will be implemented as the …

Examining The Effects Of Macrophage Populations On Cancerous Tumor Growth., Grace E. Mahlbacher

Electronic Theses and Dissertations

The most abundant immune cell types of the tumor microenvironment macrophages recruited there by tumor-eluted factors. The role of these immune cells in tumor progression, and the interplay between tumor and immune cells is an emerging field of research with potential for novel treatment strategies. Here, a TIE2 expressing macrophage (TEM) subtype is integrated into a virtual tumor model. Within the 2D microenvironment, the TEM will differentiate from an extravasated monocyte precursor, congregate around the abluminal side of the vasculature in response to a chemoattractant gradient, secrete cytokines which favor differentiation of a separate angiogenic macrophage subtype [1]. The effects …

A Novel Mra-Based Framework For The Detection Of Changes In Cerebrovascular Blood Pressure., Yitzhak Atakilt Gebru

Electronic Theses and Dissertations

Background: High blood pressure (HBP) affects 75 million adults and is the primary or contributing cause of mortality in 410,000 adults each year in the United States. Chronic HBP leads to cerebrovascular changes and is a significant contributor for strokes, dementia, and cognitive impairment. Non-invasive measurement of changes in cerebral vasculature and blood pressure (BP) may enable physicians to optimally treat HBP patients. This manuscript describes a method to non-invasively quantify changes in cerebral vasculature and BP using Magnetic Resonance Angiography (MRA) imaging.

Methods: MRA images and BP measurements were obtained from patients (n=15, M=8, F=7, Age= 49.2 …

Development Of A Directional Bone Reaming System., Richard J Ackermann

Electronic Theses and Dissertations

Preparation of long bones such as the femur or tibia for placement of intramedullary devices for the treatment of fractures usually involves reaming with a series of central cutters driven by a drill-like device with a flexible shaft over a guide wire. The reamers sequentially enlarge the intramedullary canal into a tunnel of circular cross-section and a diameter appropriate for the procedure. The current technology is concentric, meaning that the system is self-centering within the original intramedullary canal and the expansion is symmetric with respect to the original centerline. A novel system for laterally deflecting the head of a 12mm …

Psychophysiological Analysis Of A Pedagogical Agent And Robotic Peer For Individuals With Autism Spectrum Disorders., Mohammad Nasser Saadatzi

Electronic Theses and Dissertations

Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by ongoing problems in social interaction and communication, and engagement in repetitive behaviors. According to Centers for Disease Control and Prevention, an estimated 1 in 68 children in the United States has ASD. Mounting evidence shows that many of these individuals display an interest in social interaction with computers and robots and, in general, feel comfortable spending time in such environments. It is known that the subtlety and unpredictability of people’s social behavior are intimidating and confusing for many individuals with ASD. Computerized learning environments and robots, however, prepare a predictable, …

Mechanism Of Interaction Of Peptide Modified Nanoparticles With Porphyromonas Gingivalis., Ankita Jain

Electronic Theses and Dissertations

Studies suggest that P. gingivalis functions as a keystone pathogen and interacts with primary colonizers in the supragingival biofilm such as S. gordonii. This interaction contributes to the initial colonization of the oral cavity by P. gingivalis and thus represents a potential target for therapeutic intervention. We have identified a peptide (BAR) derived from the streptococcal SspB protein that functions to inhibit P. gingivalis adherence to S. gordonii. In addition, we showed that nanoparticles (NPs) functionalized with BAR inhibit this interaction more potently than free soluble peptide, possibly by promoting interaction with P. gingivalis at higher valency than …

A Cad System For Early Diagnosis Of Autism Using Different Imaging Modalities., Marwa Maher Tawfik Ismail

Electronic Theses and Dissertations

The term “autism spectrum disorder” (ASD) refers to a collection of neuro-developmental disorders that affect linguistic, behavioral, and social skills. Autism has many symptoms, most prominently, social impairment and repetitive behaviors. It is crucial to diagnose autism at an early stage for better assessment and investigation of this complex syndrome. There have been a lot of efforts to diagnose ASD using different techniques, such as imaging modalities, genetic techniques, and behavior reports. Imaging modalities have been extensively exploited for ASD diagnosis, and one of the most successful ones is Magnetic resonance imaging(MRI),where it has shown promise for the early diagnosis …

Gamma-Radiation Exposure Alters The Cardiovascular Extracellular Matrix., Nicholas Allen

Electronic Theses and Dissertations

Ionizing radiation has been associated with various cardiovascular complications; however, the associated molecular changes from radiation exposure still remain largely uncharacterized. Alterations to the cardiovascular tissue microenvironment, i.e. the extracellular matrix (ECM), directly affect the function of integrated vascular cells, including cell adhesion, potential to form vessels, and endothelial permeability, which can promote cardiovascular pathologies. The ECM is constantly remodeled in response to stimuli, such as TGF-β1, which leads to excessive ECM accumulation. We hypothesize that radiation exposure will alter the cardiovascular ECM. Human Cardiac Fibroblasts (HCFs) were utilized to produce ECM as an in vitro model to study changes …

Fabrication, Characterization And Skin Permeation Of Antoxidant-Loaded Lipid Vesicles For Transdermal Drug Delivery., Emily Martin

Electronic Theses and Dissertations

Excess production of Reactive Oxygen Species (ROS), which can be caused by radiation, cigarette smoke, and pollutants, can cause many damaging cellular effects, such as DNA damage and unregulated cell apoptosis. If left unmitigated, chronic overproduction of ROS has been linked to the formation of cancer, diabetes, atherosclerosis, rheumatoid arthritis, chronic inflammation, Parkinson’s disease, Alzheimer’s disease, multiple sclerosis, and amyotrophic lateral sclerosis. ROS is typically eliminated with antioxidants, which scavenge and inhibit the formation of ROS by becoming oxidized by the ROS and by binding to its precursors.

Curcumin is an effective lipid-soluble antioxidant agent. However, its poor bioavailability, rapid …

Development And Evaluation Of Custom Prosthetic Devices For A Companion Animal Utilizing Additive Manufacturing., Benjamin Joseph Cahill

Electronic Theses and Dissertations

BACKGROUND AND SIGINIFICANCE: Few options exist for companion animals in need of prosthetic devices. With the rise of rapid prototyping technology, the availability and customization of prosthetic devices for individual companion animals is now a viable and cost effective alternative to the current options of a peg leg prosthetic, or wheel-assisted prosthetic device. The goals of this study were to describe the (1) specific needs and (2) biomechanics of a feline with bilateral thoracic limb amputation, (3) develop custom prosthetic devices utilizing rapid prototyping technology, and (4) describe the biomechanics of a feline with bilateral thoracic limb amputation using the …

Efficacy Of Subcutaneous Electrocardiogram Leads For Synchronous Timing And Aortic Root Cannulation For Chronic Counterpulsation Therapy., Stephen R. Carnahan

Electronic Theses and Dissertations

Background: Counterpulsation devices (CPD) require electrocardiogram (ECG) lead implantation for timing device filling and ejection with the native heart. Non- implantable leads limit the scope of CPD treatment to short-term therapy. Standard transvenous/epicardial ECG leads increase the invasiveness of therapy.

Methods: To overcome these limitations, subcutaneous ECG leads were tested in chronic (n=6) and acute (n=5) bovine models. ECG waveforms from clinical- grade epicardial (control) leads and subcutaneous (test) leads were simultaneously recorded resulting in 830 data epochs (30-s each) for a total of 44,614 heart beats. Device triggering using R-wave detection was calculated for each lead type and compared. …

Development Of A Novel Device For Ventricular Assist Device Outflow Graft Anastomosis., Young Choi

Electronic Theses and Dissertations

Purpose: Left ventricular assist device (LVAD) therapy can be live-saving for advanced heart failure patients. Conventional anastomosis (surgical connection) of LVAD outflow grafts to the aorta requires aortic clamping and hand-suturing. Aortic clamping increases the risk for neurological complications. Hand-suturing may be time-consuming and requires significant surgical dexterity. There is currently no commercially available device for sutureless anastomosis of large vascular grafts (diameter > 5mm). To overcome these limitations, a prototype LVAD outflow graft anastomosis device (GrAD) that facilitates sutureless anastomosis was developed and tested to demonstrate proof-of-concept and feasibility for (1) secured attachment withstanding physiological pressures, and (2) comparable attachment …

Automated Analysis Of Cancerous Histological Samples., Thomas Allen Tennill

Electronic Theses and Dissertations

The use of immunohistochemistry has become commonplace in the field of cancer diagnosis. A major limitation to the ability to use this tool effectively is the time consuming tasks required for the analysis of data due to the fact that it is largely done by hand. Additionally, because of this, there is an inherent level of subjectivity in the results obtained from this process that may depend on who it is conducting the analysis. Therefore, there exists a need for a method that is able to quantify results from immunohistochemical techniques in a way that it is both time-effective and …

Foot Strike Patterns And Body Alignment Effects On Muscle Activity During Running., Nicole Knapp

Electronic Theses and Dissertations

The mechanics of the running stride was altered around the 1970s when shoes were released with an elevated heel (McDougall 2009). At this point in time a heel strike running style became increasingly common and has remained the popular pattern when running in traditional shoes. In fact, the exact same person has been seen to alter their stride when running in a traditional shoe where they heel strike verses barefoot where they take on a forefoot strike (Lieberman 2010).

When an individual heel strikes, the ground reaction force is greater and contains an initial peak which is void in a …

Synthesis And Functional Evaluation Of Peptide Modified Poly (Lactic-Co-Glycolic Acid) Nanoparticles To Inhibit Porphyromonas Gingivalis Biofilm Formation., Paridhi Kalia

Electronic Theses and Dissertations

Periodontal disease is an oral inflammatory disorder that afflicts roughly 46% of the adults in the U.S. Currently, treatment of periodontal disease involves the removal of plaque from the gingival pocket (with possible antibiotic treatment) and if necessary, gingival surgery. To our knowledge, no therapeutic approach exists that promotes host-biofilm homeostasis by limiting pathogen recolonization of the oral cavity after prophylaxis or treatment. The interaction of the pathogen Porphyromonas gingivalis with commensal streptococci is critical for initiation of periodontitis and represents a target for limiting P. gingivalis colonization of the oral cavity. Previous studies showed that a synthetic peptide …

Novel Biomechanical Test Method For Cancellous Bone Screws And Screw Augmentation With Cement., Kevin Lancaster

Electronic Theses and Dissertations

It is often difficult to achieve adequate screw fixation for plate constructs in fractures with poor quality bone or in metaphyseal bone with extensive bone loss or comminution[7][17]. Furthermore, rescuing or augmenting failed screw fixation in inadequate bone with various cement products has yielded mixed results when tested with pure axial pullout loading[20][21]. In most cases, plate/screw constructs experience both vertical (translational) and horizontal (pullout) forces during physiologic loading[17][20][21]. The increased use of locked screws in plate constructs has also changed the loading patterns of bone screws. For this study, a novel “toggle-out” testing method was developed to more realistically …

Development Of A Vascularized, Induced Pluripotent Stem Cell-Derived Liver-Tissue Mimic For Therapeutic Applications., Venkat Madhav Ramakrishnan

Electronic Theses and Dissertations

This dissertation describes the incorporation of several technologies (stem cells, gene therapy, tissue engineering and regenerative medicine) into a single project that aims to produce a liver-tissue mimic for therapeutic applications. The liver is arguably one of the most complex organs in the body. In addition to its remarkable capacity to regenerate, it performs a host of vital functions. As a result, its impairment has widespread systemic consequences. The work described herein focused on the liver in the context of cardiovascular disease and used the heritable disorder Familial Hypercholesterolemia (FH) as a clinical disease model. As (a) the only definitive …

Synthesis, Optimization, And Degradation Of A Fish Glue/Plga Polymer., Adam T. Schworer 1989-

Electronic Theses and Dissertations

Biomaterials used for tissue engineering, specifically polymers, come from either naturally occurring, like gelatin or collagen, or synthetic sources, such as polyesters. Often times, it is advantageous to combine biomaterials in order to maximize certain material properties, such as cellular adhesion and degradation. The purpose of this study is to provide direction for the synthesis, characterization, and a combination of a combination biomaterial between fish glue (FG), a natural source polymer, and poly-lactic-co-glycolic acid (PLGA), a synthetically made polyester. First, FG is characterized for molecular weight via Gel Permeation Chromatography, GPC. Two combination methods to mix FG/PLGA solutions are then …

Fibroblast-Derived Extracellular Matrix: An Alternative Cell Culture Substrate That Alters Lung Cancer Cell Line Phenotype., Michael Thomas Scherzer

Electronic Theses and Dissertations

Poor lung cancer survival can largely be contributed to the metastatic cells that invade and spread throughout the body. The tumor microenvironment (TME) is composed of multiple cell types, as well as non-cellular components. The TME plays a critical role in the development of metastatic cancers by providing migratory cues that change the growing tumor’s properties. The Extracellular Matrix (ECM), a main component of the TME, has been shown to change composition during tumor progression, allowing cancer cells to invade tissue and survive away from the primary cancer site. Although the ECM is well-known to influence the fate of tumor …

Development Of A Neuromusculoskeletal Computer Model In A Chondrodystrophic Dog., Gregory Russo States

Electronic Theses and Dissertations

Intervertebral disc disease (IVDD) is a naturally occurring disease in dogs that produces a spontaneous injury to the spinal cord. IVDD is characterized by mineralization of the intervertebral disc nucleus pulposus, which reduces its load bearing capacity and results in high rates of intervertebral disc herniation (IVDH). IVDH is disproportionately present in Dachshunds compared to other breeds, affecting an estimated 1 in 5 Dachshunds during their lifetime (Levine, J. M. et al., 2011). Assessment of injury severity and recovery in animal models is generally performed using a point scale, where subjects are graded according to metrics such as pain perception, …

Fabrication And Characterization Of Antioxidant Loaded Liposomes., Brian Gettler

Electronic Theses and Dissertations

Radiation exposure is both a major obstacle in space exploration and an occupational hazard for various careers causing DNA damage and ROS. In order to reduce the effects of radiation, the primary and most explored approach has been focused around mitigation in terms of exposure duration, exposure intensity, and shielding usage. In some situations none of these reduction strategies were viable and so the use of antioxidants is being explored as an alternate strategy for therapeutic purposes. One such antioxidant is curcumin, otherwise known as diferuloylmethane, is a component of turmeric with both antioxidant properties and anti-inflammatory properties. Another antioxidant …

Development Of An Application For Patient-Centered Dementia Care., David Collar Alston

Electronic Theses and Dissertations

Dementia care in the United States faces several challenges, many of them associated with managing the information that is associated with patients. Protected patient information about patients is often separated between many sources. For example, a new doctor may have information on patient medicines, but not the document associated with the caregiving goals of the patient and their family. Problems can develop if a patient is treated by a new caregiver, who may not know the patient’s treatment and behavior history. There is a strong desire for a system that can collect, organize, and analyze patient information in a user …

Pulsatile Flow Does Not Improve Efficacy In Ex Vivo Lung Perfusion., Keith A. Zoeller

Electronic Theses and Dissertations

Introduction Ex vivo lung perfusion (EVLP) has the potential to increase the donor pool for lung transplantation by facilitating extended evaluation of marginal organs. Current methodology employs continuous flow pumps for perfusion. In vivo, continuous flow has been shown to increase pulmonary vascular resistance (PVR). Thus, pulsatile flow EVLP may reduce PVR and improve organ preservation by providing physiologic flow morphology. Methods Lung blocks harvested from male, Yorkshire pigs were allocated into continuous (CF, n=3) and pulsatile flow (PF, n=4) groups. Lungs were ventilated at 4-5 mL/kg, 30% FiO2 and perfused with an acellular, albumin-based solution corrected for osmolarity, acid/base …