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Unraveling The Role Of Novel G5 Peptidase Family Proteins In Virulence And Cell Envelope Biogenesis Of Staphylococcus Aureus, Stephanie M. Marroquin

USF Tampa Graduate Theses and Dissertations

Virulence factors and the bacterial cell envelope are two important components in S. aureus pathogenesis and survival. More importantly, understanding the regulation of these cellular processes is crucial to further understanding and combating this successful pathogen. To date, numerous factors have been identified as playing a role in the regulation of Agr activity in S. aureus, including transcription factors, antisense RNAs, and host elements. Herein we investigate the product of SAUSA300_1984 (termed MroQ), a transmembrane G5 peptidase family protein, as a novel effector of this system. Using a USA300 mroQ mutant we observed a drastic reduction in proteolysis, hemolysis, and …

The Role Of Cpeb2 Alternative Splicing In Tnbc Metastasis, Shaun C. Stevens

USF Tampa Graduate Theses and Dissertations

Breast cancer is the second leading cause of cancer-related deaths for women in the U.S. Although the overall 5-year survival rate for breast cancer is 90%, this rate drops substantially for triple-negative breast cancer (TNBC) due to its high metastatic potential. Furthermore, there is a lack of targeted therapeutics for TNBC, and clinical trials have been largely unsuccessful. These characteristics validate the need for identifying novel therapeutic targets for the treatment of TNBC. The study of alternative splicing (AS) has emerged as a powerful tool to elucidate the molecular underpinnings driving cancer.

Our lab has identified cytoplasmic polyadenylation element-binding protein …

African American Female Caregivers’ Perceptions, Experiences, And Expectations Of The Special Education Process, Lenell Denise Walton

Special Education ETDs

A select few African American families who have a child with a disability have the skills and knowledge to be proactive in the special education process. Special education teams must guide and mentor African American students and their families through the special education process. However, African American families have consistently contended that their child’s special education team does not provide the services necessary to meet their child’s academic goals. This qualitative study examined the perceptions, experiences, and expectations of African American female caregivers regarding the special education process. Participants were nine African American mothers and one sibling whose family members …

Standardized Screening For Peripheral Artery Disease In Primary Setting, Tamara Tomlin

Dissertations

Problem: Peripheral artery disease (PAD) affects eight million Americans and is the third leading cause of death in the U.S. in people 60-85 years of age. Veterans are at a 45% greater risk to develop PAD than non-veterans. Left untreated, PAD can progress to hospitalization for revascularization, amputation, or death. The American Heart Association (AHA) recommends using a standardized screening tool for the diagnosis of PAD; however, a lack of standardized screening is problematic in veterans receiving proper intervention.

Methods: This quality improvement (QI) project utilized a descriptive, observational design. The American Heart Association (AHA) screening tool was administered by …

Heterosexual Parents Who Move Toward Acceptance Of Their Gay Sons, Brady Sullivan

Dissertations

Rejection of gay men by parents is a frequent occurrence. Rhoades et al. (2018) found 49% of a sample of 657 sexual minority children had experienced parental rejection due to their LGBTQ+ identity. However, the current body of literature does not discuss those heterosexual parents who initially reject their sexual minority children and then return to a place of acceptance. Within the framework of attachment theory, this dissertation used a basic qualitative approach, influenced by Grounded Theory methods, to investigate the experience of heterosexual parents who move toward acceptance of their gay son. Fifteen, white, heterosexual parents, ranging from ages …

Staying Afloat: Experiences Of Previously Incarcerated Students, Jodi Burshia, Jodi Burshia

Teacher Education, Educational Leadership & Policy ETDs

The experiences of Previously Incarcerated Indigenous Students (PIIS) need to be understood so that educators and administrators can include the experiences, perspectives, and realities of Indigenous students who have been previously incarcerated in Adult Basic Education (ABE) and collegiate programs at Tribal Community Colleges and Universities (TCUs). To date, considering and providing targeted support for this population has not been part of the focus of TCUs even when all tribal colleges work to strengthen the cultural heritage of their respective tribes (Bad Wound, 1990) and some students at TCUs are PIIS. This qualitative study offers a respectful critique of higher …

Investigating The Role And Localization Of B Cells In The Red-Eared Slider, Trachemys Scripta, Whitney Hurst

Theses and Dissertations

The immune system of humans has been studied extensively, but very little is known about the immune response in reptiles. Our lab has been working to better understand the immune system of a pond turtle, Trachemys scripta (red-eared slider). Turtles are known for their longevity and their immune system should play an important role in their ability to survive. Our main interest is understanding B cell function and location in slider turtles. B cells are a type of white blood cell that play a vital role in the immune response of humans, with the majority secreting antibodies that mark invading …

Numerical Investigation Of The Impact Of Distortion On The Hydrodynamics In The Lower Mississippi River Physical Model (Lmrpm), Ali Heidarizhaleh

LSU Doctoral Dissertations

The Lower Mississippi River Physical Model (LMRPM) is a distorted-scale, 1:6000 horizontal and 1:400 vertical, movable-bed model that is being used to complement ongoing numerical and field studies directed at studying how non-cohesive sediment transport will be impacted by future changes in relative sea level rise and river discharges, as well various management strategies, such as river sediment diversions, in the lower ~190 miles of the Mississippi River The LMRPM was designed using Froude number (Fr) similarity between the prototype and the model, while the flow Reynolds number is relaxed, but determined to be high enough to ensure rough turbulent …

Fighting Mass Diffusion Of Fake News On Social Media, Abdallah Musmar

USF Tampa Graduate Theses and Dissertations

Fake news has been considered one of the most challenging problems in the last few years. The effects of spreading fake news over social media platforms are widely observed across the globe as the depth and velocity of fake news reach far more than real news (Vosoughi et al., 2018). The plan for the following dissertation is to investigate the mass spread of fake news across social media and propose a framework to fight the spread of fake news by mixing preventive methods that could hinder the overall percentage of fake news sharing. We plan to create a study on …

Incarnational Literacy: Multimodal Explorations Through Virtual Reality And Transformational Autoethnography, Csaba Osvath

USF Tampa Graduate Theses and Dissertations

Incarnational literacy is a multimodal, maker-centered, arts-based literacy engagement. As an emerging method and theoretical orientation, it incorporates core principles and transformative tools from the domains of creativity, arts-based inquiry, literacy, and spirituality. This experimental, multimodal dissertation chronicles the conception and ongoing development of incarnational literacy through a series of autoethnographic narratives, demonstrating the existential benefits of the integrated practice of artmaking and literacy engagements. This study also highlights the role and function of videogames and virtual reality experiences, revealing the benefits of introspective gameplay as tools of inquiry and meaning-making - further enriching the developing practice and domain of …

Understanding Nonprofit Boards: An Exploratory Study Of The Governance Practices Of Regional Nonprofits, Susan Ryan Goodman

USF Tampa Graduate Theses and Dissertations

Many researchers have catalogued best practices of board governance. Most of the existing research employed surveys to review compliance with these practices, and the results are highly quantitative as well as self-reported. This study used interviews of board members representing a cross-section of nonprofits to examine conformity with recommended governance best practices and procedures as well as to provide insight into how these practices have changed during their tenure.Semi-structured interviews were conducted with 18 nonprofit board members in the Tampa Bay area. Using the thematic coding approach proposed by Braun and Clark (2006), four themes emerged from the data: Recruiting, …

Efficient Management Of Nitrogen And Phosphorus At Centralized Water Reclamation Facilities, Helene Kassouf

USF Tampa Graduate Theses and Dissertations

A variety of process configurations are employed at municipal water reclamation facilities (WRFs), such as 5-stage Bardenpho, oxidation ditch, modified Ludzack-Ettinger (MLE) process, and sidestream recycle. However, all of these configurations face certain challenges in achieving optimum treatment of nitrogen and phosphorus. In this dissertation, the driving objectives were to: (1) quantitatively assess the fate of nitrogen and phosphorus (mass fluxes) at a treatment facility that employs biological nutrient removal, aerobic digestion, and sidestream recycle, (2) evaluate the performance of a microbial fuel cell (MFC) as a technology to remove nitrogen from sidestreams at a treatment plant that employs that …

Space-Time Coded Artm Cpm For Aeronautical Mobile Telemetry, Chad Carl Josephson

Theses and Dissertations

This dissertation explores the application of Silvester's space-time block code to the multi-index CPM called "ARTM CPM" in the IRIG 106 standard to solve the "two antenna problem"---the use of two transmit antennas to provide full spatial coverage on an airborne test article and the accompanying self interference due to different delays between the two transmit antennas and the ground-based receive antenna. A symbol-level encoding scheme is derived that allows the burst-based space-time block code to operate in a continuously streaming mode. The results show that the space-time block code can solve the two antenna problem with differential delays, but …

Assessment Of Executive Function Using A Series Of Operant Conditioning Based Tasks In T1dm Rodents, Kevin T. Murphy

Electronic Thesis and Dissertation Repository

This study examined the impact of Type 1 Diabetes Mellitus (T1DM) on executive function using a series of operant conditioning based tasks in rats. Sprague Dawley rats were randomized to either non-diabetic (n = 12; 6 male) or diabetic (n = 14; 6 male) groups. Diabetes was induced using multiple low-dose streptozotocin injections. All diabetic rodents were insulin-treated using subcutaneous insulin pellet implants. At week 14 of the study, rats were placed on a food restricted diet to induce 5 - 10% weight loss. Rodents were familiarized and tested on a series of tasks that required continuous adjustments to novel …

The Synthesis And Application Of Polyglyoxylamides, Quinton Ea Sirianni

Electronic Thesis and Dissertation Repository

Self-immolative polymers (SIPs) are a subset of degradable polymers that can be triggered to fully depolymerize with a single stimulus event. This amplifying behaviour makes them ideal for real-world applications including sensors, recyclable plastics, and drug delivery vehicles. Polyglyoxylates (PGs) are a class of SIPs that can be synthesized in a one-pot reaction and designed to respond to different stimuli including light, heat, reduction, and oxidation. However, with the exception of poly(ethyl glyoxylate) (PEtG), the various glyoxylate monomers must be prepared and purified before polymerization, which can be difficult. Furthermore, PGs typically possess properties such as water-insolubility and low glass …

Examining Public Health Risk Communication Via Social Media By Provincial And Local Health Authorities In Ontario During The Covid-19 Pandemic, Marc Resendes

Electronic Thesis and Dissertation Repository

Risk communication campaigns are essential during public health crises to inform the public about ways to mitigate, alleviate and manage potential risks. The purpose of this study was to describe risk communication on social media by Ontarian health authorities amid COVID-19, in addition to examining the strategies that guided their social media use. This was completed through (a) a narrative review of risk communication literature; (b) a qualitative content analysis of select health authority Twitter messaging following three major COVID-19 milestones; and (c) key informant interviews with those coordinating social media responses to COVID-19. Information giving and news updates were …

Fórmulas De Tratamiento En Ocaña, Colombia, Liceth Bohorquez Leon

Electronic Thesis and Dissertation Repository

The paradigm of forms of address has been a recurrent topic of study from a sociolinguistic perspective. Voseo, tuteo and ustedeo are the forms of address used in the Hispanic world. Voseo expreses confidence and familiarity, while tuteo same as voseo, denotes confidence and familiarity. Finally, ustedeo has a connotation of formality, respect and distance. This thesis aims to examine how forms of address are used in Ocaña, Colombia, and the relationship between their use and the social factors that affect the choice of pronoun. To achieve this aim, a sociolinguistic survey was applied to 88 participants. The …

Enhance Dopamine Autoreceptor Functioning During Blockade Of Dopamine Transporters, Rachel Laura Pace

Electronic Theses and Dissertations

Dopamine autoreceptors (DARs) and dopamine transporters (DATs) influence dopamine transmission in the brain's mesolimbic pathway. Prior studies have focused on how activation of DARs influences trafficking of DATs, but how DATs influence DARs remains unclear. Male and female C57BL/6J mice received daily injections of either cocaine (DAT blocker) or saline for seven days then underwent stereotaxic surgery to obtain fixed potential amperometric recordings of DAR-mediated dopamine release. All mice received a mid-surgery injection of cocaine, and DAR-mediated dopamine release was assessed once more during maximal DAT blockade. The current study found that DAR functionality was increased during peak cocaine effects, …

Simulation Of Bulk Evaporation And Condensation In Cryogenic Propellant Tanks Using The Energy Of Fluid Method, Elijah Gasmen

Electronic Theses and Dissertations

The design of cryogenic propellant storage systems for long duration space missions relies on accurate prediction of tank self-pressurization. Incident solar radiation heats the cryogenic liquids in the tank over time, vaporizing the cryogenic liquid. As the liquid vaporizes, the tank pressure increases. The objective of the current research is to develop a finite volume based Compuational Fluid Dynamic (CFD) model of tank pressurization in reduced gravity using an Energy of Fluid (EOF) method. A commercially available CFD model is significantly enhanced to include the EOF method, which will solve the energy equation in terms of internal energy. Model validation …

Pressure Retarded Osmosis: A Potential Technology For Seawater Desalination Energy Recovery And Concentrate Management, Joshua Benjamin

USF Tampa Graduate Theses and Dissertations

Currently, a significant challenge with reverse osmosis-based desalination is reducing the energy consumption and environmental impacts of the process. This project analyzed the viability of using pressure-retarded osmosis (PRO) for energy recovery in seawater desalination facilities using brine concentrate (the draw solution) and other water sources (the feed solution) such as wastewater effluent. The primary goal of this project is to decrease the cost and overall energy consumption of seawater desalination through PRO-based energy recovery. Process modeling, statistical and sensitivity analysis, energy and cost analysis, geospatial and GIS analysis, laboratory-scale testing, water quality analysis, SEM-EDS microscopy, computational fluid dynamics (CFD), …

Ecological Genomics Of Two Coastal Foundation Plant Species, Jeannie Mounger

USF Tampa Graduate Theses and Dissertations

I was born and raised in rural Florida on a tributary of the Withlacoochee River. “Withlacoochee” comes from a series of Muscogee Creek words that together mean “big little water,” possibly owing to the seasonal fluctuations in the breadth and flow of the river or to the chain of lakes that in part form it. The name contains within it an understanding of the capacity of land and water to undergo great shifts. It conjures images of mutable boundaries, of water covering and receding across the landscape over and over again. Many of my clearest memories from childhood are related …

Resolution Of Lava Tubes With Ground Penetrating Radar, Sanaz Esmaeili

USF Tampa Graduate Theses and Dissertations

Finding habitable places to keep astronauts safe from surface radiation, magnetic storms, and temperature fluctuations will be an important component of future planetary exploration missions. Remote sensing surveys on other planets indicate the presence of lava tubes. Ground Penetrating Radar (GPR) has shown great potential for detection of tunnels in terrestrial environments. In this research, the capabilities of this near surface rapid exploratory tool for detection of lava tubes are investigated. This dissertation describes terrestrial examples of how GPR can be utilized to explore tubes and addresses the capabilities of GPR for resolving tube-related features such as the floor, fractures, …

Get Into It: Unpacking The Relationship Between Hegemonic Norms And The Race And Gender Of Pop Artists, Bailey Shelton

Master's Theses

Many contemporary popular musical artists are victims of media controversy while they simultaneously make musical chat history. As a culture founded and sustained by a white supremacist capitalistic patriarchy, race and sex appear to influence the way hegemonic in-groups treat minorities and the way minorities are perceived and live within the confines of said hegemony. With the context of understanding feminist theories, hegemonic norms, parasocial relationships, celebrity appeal, and norms, the researcher wanted to understand if there are clear relationship among these frameworks in modern society. This study employed a 2x2 2between subjects factorial design method to gather information about …

Teacher Leadership In The Context Of Chinese Education, Chudi Zhou, Qian Yang, Ying Dong, Xiaoying Wang

Dissertations

In response to addressing the increasing requirements to enhance the competencies of students and teachers, as well as the quality of schools in the global environment, the need for fostering teacher leadership has been proposed by many scholars and practitioners. School context is regarded as an important influence on the successful development of teacher leadership. While many studies related to teacher leadership have been reported in Western countries, a limited number of studies have been reported within the Chinese educational context. We also observed that among the limited studies, there was a lack of empirical research on how schools provide …

Letitia Carson In Court: African American Women, Property, And Wages In The Pacific Northwest, Stephanie Marie Vallance

Dissertations and Theses

Letitia Carson arrived in Oregon from Missouri in 1845, accompanied by David Carson and their newborn child, a daughter named Martha. The Carsons settled in the Soap Creek Valley and took advantage of Oregon's Provisional Government's donation land claim program, living on 640 acres in the newly formed Benton County with Martha and a second child, a son named Adam, born a few years after arriving in Oregon. Within ten years, however, David would be dead and Letitia would be dispossessed of all property and belongings. A former slave, Letitia had little social standing in the new territory and no …

Explorations And Developments In Multifunctionality, 3d Printing, And Accelerated Aging For Thermoplastics And Thermosets, Zachary Brounstein

Nanoscience and Microsystems ETDs

If the world is to progress through the fourth industrial revolution, rapid advances in materials science must complement new technological feats in manufacturing, computation and device functionality, and a broader understanding of how materials behave as they age and degrade. To this effect, much effort has been directed towards polymeric materials to address and solve many problems in this new era. This includes developing polymer composites that incorporate fillers to imbue multifunctionality, fabricating novel formulations for additive manufacturing, and conducting aging studies to assess the performance of these materials over long time spans. Building and expanding on the latest research …

The Photophysical Studies Of Transition Metal Polyimines Encapsulated In Metal Organic Frameworks (Mof’S), Jacob M. Mayers

USF Tampa Graduate Theses and Dissertations

Light harvesting systems provide a platform that converts solar energy into other forms of energy. One of the most common examples of photon capturing and conversion into chemical energy is observed in photosynthetic organisms in both Eurkaroyic and Prokaryotic domains. Nature provides a model for successful light harvesting platforms which includes the compartmentalization of antenna complexes that contain separated donor and acceptor pairs that participate in efficient electron transfer processes. In order to mimic such systems, crystalline porous materials that exhibits regular cavities and pore dimensions provides an excellent starting place. Metal organic frameworks (MOFs) are a class of porous …

Oppression, Resistance, And Empowerment: The Power Dynamics Of Naming And Un-Naming In African American Literature, 1794 To 2019, Melissa "Maggie" Romigh

USF Tampa Graduate Theses and Dissertations

Oppression, Resistance, and Empowerment: The Power Dynamics of Naming and Un-naming in African American Literature, 1794 to 2019 researches and discusses the way African American authors both discuss naming and un-naming in their works and the way they use naming in their works to illustrate the dynamics of power in relationships—racial, familial, gender-related, work-related, etc. Chapter 1 focuses on the earliest forms of African American literature, memoirs in particular, also known as “slave narratives.” In their memoirs, many of those men and women who were formerly enslaved wrote about having their names taken from them and replaced with names chosen …

Copper Electrodeposition Assisted By Hydrogen Evolution For Wearable Electronics: Interconnections And Fiber Metallization, Sabrina M. Rosa Ortiz

USF Tampa Graduate Theses and Dissertations

The current project focused on studying an innovative and novel method, called hydrogen evolution assisted (HEA) electroplating, for rapid and localized electrochemical copper deposition that can be applied for integration of electronic circuits to printed circuit boards (PCBs) and fabrics for developing smart textile (e-textile) and advanced wearable electronics. This method has shown a great enhancement to the deposition rate of copper compared to the conventional galvanostatic electroplating method, opening new venues for patterning conductive circuit layouts on fabrics and for the direct integration of devices into fabrics. While due to the slow growth rate and low mechanical flexibility of …

Engagement And Meaningfulness As Determinants Of Employee Retention: A Longitudinal Case Study, Calvin Williams

USF Tampa Graduate Theses and Dissertations

The lack of financial resources has long challenged government institutions to move employee wages toward the market rate average for hourly and salaried positions. This area of public employment, where motivation other than a pay increase is necessary due to constrained budgets, is not well studied or understood. The primary purpose of this longitudinal case study is to investigate factors that influence Administrative Service employees and increase their level of retention at the University of South Florida (USF), a state funded university. The objective of the case study was to better understand how enhanced psychological meaningfulness, and increased employee engagement …