Food Biotechnology Commons^™

Analysis On Quality In Three Kinds Of Basal Tea Of Noval-Jasmine Tea Beverage, Zhang Fen, Chen Jiaxian, Yuan Dongyin, Zhang Xuhao, Wen Lixiang

Food and Machinery

Objective: This study aimed to explore the quality features of basal tea of noval-jasmine tea. Methods: Taking three kinds of basal tea（Fujian Xianghao, Yunnan Maojian, Sichuan Xiangming）of noval-jasmine tea beverage scenting tea dhool from Fujian, Yunnan and Sichuan as the research objects, the quality of the base tea including main biochemical components, taste, aroma were analyzed by electronic tongue, solid phase microextraction combined with gas chromatography-mass spectrometry (GC-MS), sensory evaluation and chemical analysis. Results: Water extracts of three kinds of basal tea was 49.40%~51.40%, tea polyphenols was 18.00%~23.20%, caffeine was 3.20%~4.30%, free amino acid was 3.32%~3.50%. The main biochemical components …

Study On The Effect Of Ultrasonic Aging Of Crude Baijiu After Second Distillation, Zhang Yingying, Chen Xuepeng, Yu Yougui, Tan Wenjun, Wu Jinlan, Dai Shan, Zheng Qing

Food and Machinery

Objective: Accelerated manmade aging of base liquor after heavy distillation. Methods: A single factor experimental design method was adopted based on the study of impurity removal in second distillation of Luzhou-flavor crude Baijiu. The changes in the content of total acids and total esters in the liquor were used as indicators to preliminarily explore the effect of ultrasonic treatment conditions on the crude Baijiu. Results: The results showed that: among the three single factors of ultrasonic treatment power, temperature and time, there were varying degrees of differences between the levels of each individual factor; At an ultrasonic frequency of 45 …

Exploring The Nutritional, Antioxidant, And Lipid-Lowering Properties Of Saba Banana (Musa Acuminata X Balbisiana Bbb Group) Peels, Paul Alteo Bagabaldo, Liezl M. Atienza, Katherine Ann T. Castillo-Israel, Aimee Sheree A. Barrion, Antonio C. Laurena, Maria Amelita C. Estacio

The Philippine Agricultural Scientist

This study investigated the nutritional and functional components of ‘saba’ banana peel (SBP) to determine its health-promoting potential for special food applications. SBP has undergone analyses measuring proximate composition, vitamin C, antioxidant activity, phytochemical content, estimated glycemic index (eGI), pancreatic lipase inhibition, and cholesterol micellar solubility inhibition. Data were processed using SPSS v20 and expressed as mean ± SD. Proximate analysis showed that SBP contained 83.83 ± 1.06% moisture, 6.74 ± 0.83% crude fat, 5.58 ± 0.24% crude protein, 9.97 ± 0.04% total ash, 9.91 ± 0.10% crude fiber, and 60.08% nitrogen-free extracts. Vitamin C content was found to be …

Development Of An Intact Mass Spectrometry Method For The Detection And Differentiation Of Major Bovine Milk Proteins, Emily F. Harley-Dowell

Department of Food Science and Technology: Dissertations, Theses, and Student Research

The authentication of products with claims regarding protein sources or compositions is a challenge for traditional analytical methods, which generally lack the required specificity whole protein analysis can provide. For example, the establishment of milk as “A2” is achieved through genetic testing of cows before milk production, with no methods to authenticate milk products themselves. Establishment of A2 milk is completed through genetic testing of the cows before milk production, but with no methods to authenticate the milk products themselves. Intact protein mass spectrometry (MS) has the potential to directly authenticate protein products, including specific proteoform claims. The development of …

Village-Level Processing Of Pangi (Pangium Edule Reinw.) Seeds By The Obu Manuvu Improves The Edibility And Level Of Some Nutritional Factors [Research Note], Rovi Gem E. Villame-Gayagas, Jennifer P. Fronteras, Maria Christna D. Nacar, Aaron P. Lorilla, Pedro A. Alviola Iv

The Philippine Agricultural Scientist

Pangi (Pangium edule Reinw) seed is known to be a poisonous material. However, it becomes safe for consumption after processing. This study determined the effect of village-level processing by the Obu Manuvu community of Marilog District, Davao City, Philippines on the antinutrient and nutritional composition of Pangi seeds. Processing methods involved washing with running water for about 15 s, boiling in water for 30 min, and soaking in flowing water in a nearby river overnight. Results showed that after processing, the levels of antinutrients such as cyanogenic glycosides and oxalates were reduced significantly (p < 0.05) by 99.15% and 78.23%, respectively. Further, the tannin content was also significantly reduced by 96.15%. The crude fat content significantly increased (p < 0.05) by about 54%. Crude ash and fiber decreased, while protein did not change significantly (p < 0.05). Minerals such as calcium and zinc increased (p < 0.05) by 221.43% and 64.39%, respectively while the iron and manganese levels remained unaffected. Findings of the study suggested that the processing method by the Obu Manuvu effectively reduces the level of antinutrients, thereby improving the edibility and safety of Pangi seeds. Further, the processing method improves the level of some nutrients, specifically crude fat, calcium, and zinc.

Valorisation Of Underutilised Dairy Waste Residues: Production Of Lactic Acid Through Microbial Fermentation, Chatan Rai Surana

ORBioM (Open Research BioSciences Meeting)

Annually, approximately 190 million tonnes of liquid waste or co-products is generated by the dairy sector across the globe. These waste streams are nutrient-rich and currently underutilised which gives scope to transform them through microbial fermentation to produce economically valuable products and reduce their negative environmental impact. Such an approach can contribute to the circular bioeconomy by making food production systems more sustainable.

In this study, whey from acid casein hydrolysis (acid whey) and salty whey from Cheddar cheese manufacture were evaluated as feedstocks for production of lactic acid. A total of 466 lactic acid bacteria strains were screened on …

Determination Of Rutin, Hyperoside, Quercetin In Xiang Lotus By Hplc, Zhong Feifei, Li Jing, Lei Deqing, Yang Yun, Wu Jianfang, Ouyang Li

Food and Machinery

Objective: To establish a HPLC method for simultaneous determination of rutin, hyperoside, quercetin in Xiang lotus. Methods: The HPLC method was performed on an Agilent ZORBAX SB-C₁₈ column (4.6 mm×250 mm, 5 m) with gradient elution of methanol and 0.6% phosphoric acid solution. The flow rate was 1.0 mL/min. The column temperature was 30 ℃ and the detection wavelength was set at 255 nm. Results: The contents of rutin, hypericin and quercetin in 20 batches of lotus samples were 1.32～2.06, 1.36～2.43, 1.41～3.26 mg/kg, respectively. The linear range of the concentration of rutin, hypericin and quercetin was 5～200 g/mL ( …

Nanozymatic Activity Of Zr-Based Mofs: Tuning The Coordinatively Unsaturated Metal Sites For Enhancing Hydrolytic Activity Toward Peptide Bonds, Xu Keyu, Xia Liwei, Xing Keyu, Cheng Yunhui, Xu Zhou

Food and Machinery

Objective: To develop efficient and stable artificial proteases and investigate the relationship between coordinatively unsaturated metal sites and hydrolysis activity of peptide bond. Methods: Three zirconium-based metal-organic frameworks （Zr-MOFs） with different coordinatively unsaturated sites （12-connected Zr-MOF, 6-connected Zr-MOF, and 4-connected Zr-MOF） were prepared to modulate protein hydrolase activity. The protein hydrolase activity of three Zr-MOFs was evaluated by the hydrolysis rate of bis-glycopeptide （Gly-Gly） as an indicator. Subsequently, soy protein, surimi protein and casein were hydrolyzed using the best performing protein hydrolase nanoenzymes, and the hydrolysis products were separated using SDS-polyacrylamide gel electrophoresis, and the hydrolysis efficiency of the three …

Effects Of Three Media On The Isolation Of Two Kinds Of Antimicrobial-Resistant Bacteria From Chicken Products, Huang Liujuan, Fan Yanli, Zhong Hai, Jiang Jing, Shao Yi

Food and Machinery

Objective: To evaluate the isolation effects of different media on antimicrobial risistant （ART） bacteria flora from the surface of cold fresh and cooked chicken products. Methods: Based on high-throughput sequencing technology, the flora structure and abundance of tetracycline-resistant （T^r） and sulfamethazole-resistant （S^r） bacteria isolated by brain heart infusion （BHI）, tryptic soy agar （TSA） and plate count agar （PCA） media was comparatively analyzed. Results: Among the 101 genera of ART bacteria from cold fresh chicken samples, 30, 5 and 3 genera of T^r bacteria were isolated only on BHI, TSA and PCA media respectively, while the …

Changes Of Chemical Components Of Tibetan Tea During Pile Fermentation Process, Deng Junlin, He Yanghang, Chen Jian, Liu Gang, Xia Chen

Food and Machinery

Objective: The changes in the main chemical components in water extracts of Tibetan tea during the pile fermentation process were investigated. Methods: Research was done on tea samples and water extracts from various fermentation stages of Tibetan tea. Soluble solids, soluble sugars, total polyphenols, total flavonoids, and in vitro antioxidant activity (ABTS and DPPH) were all examined. Additionally, ultra-performance liquid chromatography (UPLC) was used to find theophylline, caffeine, gallic acid (GA), and 8 catechins monomers. By using enzyme-linked immunoassay, the amount of theaflavin, thearubigin, and theabrownin were determined. Results: The soluble solids in Tibetan tea reduced and then slightly increased …

Evaluation Of The Performance Of Enrofloxacin In The Detection Of Egg By Quantum Dot Microsphere Immunochromatography Strip, Zhang Fusheng, Wang Yuchen, Lu Lisheng, Deng Jiajun, Chen Junsong, Yang Linfen, Guo Jing, Wu Renwei, Lai Weihua

Food and Machinery

Objective: This study aimed to realize rapid on-site screening of enrofloxacin in eggs. Methods: According to the requirements of the “Maximum residue limit of 41 veterinary drugs in the national food safety standard” and the “Opinions on regulating the use of rapid food testing” issued by the State Market Supervision and Administration in January 2023, the spiked samples at three concentration levels of enrofloxacin were prepared. Fifty blank samples and fifty spiked samples at a concentration level of 0.5 time the standard limit requirement (5 μg/kg) were used to evaluate the false positive rate of the rapid detection method. Fifty …

A Fluorescence Elisa Based On Dsdna-Cuncs For The Detection Of E. Coli O157:H7 In Milk, Cao Wenkai, Shan Shan, Liu Daofeng, Peng Juan, Xing Keyu, Lai Weihua

Food and Machinery

Objective: This study aimed to developed a novel fluorescent enzyme-linked immunosorbent assay method for the sensitive detection of E. coli O157:H7 in milk samples. Methods: The pyrophosphate-Cu²⁺ coordination complex was hydrolyzed by alkaline phosphatase, and Cu²⁺ was released from copper nanoclusters as signal reporting probes. Key factors such as Cu²⁺ concentration, pyrophosphate concentration, and DNA template concentration and length were optimized. Results: Under the control of the optimized conditions, the method exhibited a good linear relationship (R²=0.980 8) with the target E. coli O157:H7 in the range of 5×10⁴~1×10⁸ CFU/mL. The …

Determination Of Acrylamide In Fried Foods By High Performance Liquid Chromatography Based On Quechers, Dong Wenjing, Dai Jinbo, Nie Rongrong, Shen Jie, Ye Caiping, Cai Zhitao

Food and Machinery

Objective: This study aimed to popularize the detection capability of acrylamide in fried foods and optimize the pre-treatment process and the reliance on expensive instruments. Methods: The acrylamide in fried foods were extracted by methanol as extraction solvent and used C₁₈ and Z-Sep⁺ as clean up materials. Then separated it with C₁₈ liquid chromatography column by using water as mobile phase for isocratic elution and detected at wavelength 197 nm. Results: This method showed a good linearity (R²>0.999) of acrylamide in the range of 0.1～2.0 mg/L and the limits of detection was 0.007 mg/kg. …

Determination Of Zn2+ Cd2+ Pb2+ In Food Base On Bi-Co-Btc Electrochemical Sensor, Ding Kewu, Dai Lili, Huang Dihui, Sun Zhongwei, Ye Ruihong

Food and Machinery

Objective: This study focused on the preparation of a new electrochemical sensor for detection of heavy metal content in food. Methods: In this study, a new type of bismuth-based metal-organic framework was prepared by hydrothermal method. 1,3,5-benzenetricarboxylic acid （H₃BTC） was used as a ligand, which supplemented by bismuth nitrate pentahydrate [Bi（NO₃）₃ 5H₂O] and cobalt nitrate hexahydrate [Co（NO₃）₂·6H₂O] metal salts to prepare novel bismuth-based metal-organic frameworks. Bi-based metal-organic framework composites supported by multi-walled carbon nanotubes were prepared by ultrasonic self-assembly. The surface of glassy carbon electrode （GCE） was …

A Study On The Application Of Non-Prosecution Of Criminal Compliance By Food Production And Operation Enterprises, Cai Kunpeng

Food and Machinery

With the current criminal compliance pilot, how to apply it correctly in the field of food safety is a new research direction. Starting from the criminal behavior of substantive law, this paper probes into the rationality of applying compliance non-prosecution in food production and operation enterprises, and through the analysis of the application of criminal compliance non-prosecution, puts forward the measures for applying the system in the field of food safety.

Simulation Analysis Of Flow Channel Of Different Twin-Screw Extruder With Different Groove Depth, Zhang Yiming, Huang Zhigang, Yang Yanan

Food and Machinery

Objective: The flow channel distribution of polylactic acid （PLA） in different groove depths of a double screw extruder was discussed. Methods: Three kinds of screw elements with different depth of screw groove and their corresponding flow channel models were established by Solidworks software. Polyflow fluid simulation software was used for numerical simulation, and FieldView software was used for numerical analysis of post-processing results. The pressure field, shear rate field, viscosity field and residence time distribution curve （RTD） of polylactic acid （PLA） in three different screw extruders with different groove depths were studied. Results: The depth of the screw groove was …

Prediction Method Of Fragrant Pear Weight Based On Kinect Camera, Zhang Runzhi, Zhang Xiao, Wu Gang

Food and Machinery

Objective: Aiming to obtain the weight of fragrant pear quickly to provide a basis for developing the fragrant pear grading device. Methods: This method acquired RGB-D images of fragrant pear by Kinect camera and converted them into point cloud data. The point cloud data was pre-processed and interpolated to generate a fragrant pear model. Then calculated the size parameter of the fragrant pear model. Finally, using the fragrant pear's volume predicted its weight. Results: Experimental results showed that the average relative error of the volume was 2.8%. Then the volume of fragrant pears was calculated by the error-compensated measurement method …

Fruit Classification System Based On The Pressure Sensor Coupled With Support Vector Machine, Wang Feifei, Liu Peng, Sun Fengwei, Zhou Qiong

Food and Machinery

Objective: To improve fruit classification accuracy while considering low computational cost and low sensor cost. Methods: A fruit classification system based on capacitive pressure sensor was proposed. The system used support vector machine algorithm with a Gaussian kernel function (GKF-SVM) to classify fruits. The capacitive pressure sensors used were made of thin copper sheets and a layer of vinyl acetate, and these sensors were fixed to the thumb and index finger of a polyamide spandex glove that simulates a robotic hand. The obtained capacitance value was expressed in the form of digital level, and the data was extracted by data …

Identification Method Of Canned Food For Production Line Sorting Robot Based On Improved Pso-Svm, Gao Haiyan, Gao Jinyang, Wang Weicheng

Food and Machinery

Objective: To solve the problems of poor accuracy and low efficiency in target recognition methods for existing sorting robots in food production lines. Methods: On the basis of the analysis of the binocular vision food sorting system, a combination of improved particle swarm optimization algorithm and support vector machine was proposed for target recognition of food sorting robots. By improving the particle swarm optimization algorithm to optimize support vector machine parameters, an optimized support vector machine classification model was obtained. The classifier was trained for both global and local features, and feature weight coefficients were dynamically assigned to obtain the …

Research Progress Of Food Packaging Defect Detection Based On Machine Vision, Ge Minghui, Zhang Jun, Lu Huijuan

Food and Machinery

Food packaging can develop defects during the production process due to various factors. The types of packaging defects are numerous with complex background. Machine vision detection, which uses visual imaging and computer information processing to complete tasks such as identification, detection, and measurement of packaging, has faster execution speed and higher accuracy compared to traditional manual inspection. This can significantly improve the degree of production automation. This article analyzes the common defects in food packaging and their causes, introduces traditional machine vision detection algorithms, and explores the research application of deep learning algorithms in food packaging defect detection. It also …