Open Access. Powered by Scholars. Published by Universities.®

Food Biotechnology Commons™

Open Access. Powered by Scholars. Published by Universities.®

5,274 Full-Text Articles 15,980 Authors 1,013,149 Downloads 56 Institutions

All Articles in Food Biotechnology

Faceted Search

5,274 full-text articles. Page 185 of 262.

Screening And Safety Evaluation Of Lactic Acid Bacteria Producing Fibrinolytic Enzyme, YIN Mengwen, ZHOU Qian, LEI Chan, HU Xinjie 2017 College of Food Sichuan Agriculture University, Ya’an, Sichuan 625014, China

Screening And Safety Evaluation Of Lactic Acid Bacteria Producing Fibrinolytic Enzyme, Yin Mengwen, Zhou Qian, Lei Chan, Hu Xinjie

Food and Machinery

In this study, 2 strains of Enterococcus faecalis HYD09 and HYN06 isolated from traditional fermented food were used to determine the enzyme activity of fermentation supernatant, and evaluate the basic safety of the strains. The safer strain were tested for gastrointestinal viability; meanwhile, the fermentation supernatant was subjected to protease; the crude enzyme was obtained from the fermentation broth and its activity was measured by Native-Page. The results showed: the enzyme activities of HYD09 and HYN06 fermentation supernatant were 115.3 U/mL and 107.2 U/mL, respectively; HYD09 and HYN06 indole test, nitrate reductase assay, amino acid decarboxylase test hemolysis test were …


Optimal Cultivation Conditions For Streptococcus Salivarius Bd3900 To Express Substances Capable Of Inhibiting Streptococcus Mutans Biofilm Formation, YU Yanan, WU Zhengjun, HAN Jin 2017 State Key Laboratory of Dairy Biotechnology, Shanghai 200436, China; Shanghai Engineering Research Center of Dairy Biotechnology, Shanghai 200436, China

Optimal Cultivation Conditions For Streptococcus Salivarius Bd3900 To Express Substances Capable Of Inhibiting Streptococcus Mutans Biofilm Formation, Yu Yanan, Wu Zhengjun, Han Jin

Food and Machinery

In order to increase the metabolites of the strain BD3900 inhibiting the S. mutans biofilm formation, the inhibition rate of biofilm formation by Streptococcus mutans was used as indexes. The effects of the cultivation time and temperature, the carbon resources, the concentration of sucrose and the inoculum amount were investigated. The results showed that optimal cultivation conditions were composed of a cultivation period of 16 h, a cultivation temperature of 34 ℃, and a sucrose concentration of 1 g/100 mL. Under the optimized cultivation conditions, the inhibitory ability of the strain BD3900 on the formation of S. mutans biofilm was …


Rapid Quantitative Detection Of Trehalose Syrup Component Based On Near Infrared Spectroscopy, ZHENG Ruina, XIE Ding, YANG Qianyuan 2017 College of Chemistry and Biology, Changsha University of Science and Technology, Changsha, Hunan 410114, China

Rapid Quantitative Detection Of Trehalose Syrup Component Based On Near Infrared Spectroscopy, Zheng Ruina, Xie Ding, Yang Qianyuan

Food and Machinery

A rapid detection method of using the near-infrared spectra(NIRS)to determine the concentration of syrup (trehalose, maltose and glucose ) in producing trehalose by synthase was established. The 65 NIRS samples of trehalose syrup were divided into a calibration set of 48 samples and a prediction set of 17 samples. Calculation results showed that the first derivative and savitzky-golay filter pretreatment is optimal, and the partial least squares (PLS) was more robust and predictive than the principal component regression (PCR) in building model by TQ analyst. Using PLS, 1D and savitzky-golay filter combination pretreatment model could not only reduce the background …


Determination Of Hydrazodicarbonamide In Bread By High Performance Liquid Chromatography In Tandem Atmospheric Pressure Chemical Ionization Source Mass Spectrometry, ZHAO Youyou, HUA Jin, GAO Yuanhui, ZHANG Qi, GUO Haiqian, ZHANG Xinxin, YANG Puchen 2017 Shanxi Entry-exit Inspection and Quarantine Bureau, Taiyuan, Shanxi 030051, China

Determination Of Hydrazodicarbonamide In Bread By High Performance Liquid Chromatography In Tandem Atmospheric Pressure Chemical Ionization Source Mass Spectrometry, Zhao Youyou, Hua Jin, Gao Yuanhui, Zhang Qi, Guo Haiqian, Zhang Xinxin, Yang Puchen

Food and Machinery

A method of determining the content of hydrazodicarbonamide(HDC) in bread by high performance liquid chromatography in tandem atmospheric pressure chemical ionization source mass spectrometry (HPLC-APCI-MS) was established. Using 80 ℃ water as extractant, N-hexane extraction was used to remove sample impurities. And then the samples purified were separated by using Xbridge BEH Prep OBD Amide column. The gradient elution was performed by using wateracetonitrile (37) (v/v), with the flow velocity 0.6 mL/min. The ionization were achieved by using APCI source positive ion mode with MRM scan mode. The internal standard calibration curves were used for quantitative. The method of adding-recovery …


Measurement Uncertainty Of 3 Alkaloids In Soup Flavor Seasoning By Lc-Ms/Ms, MA Lixing, LU Xu, CHEN Richun 2017 Fujian Saifu Food Inspection Research Institute Co., Ltd., Fuzhou, Fujian 350011, China

Measurement Uncertainty Of 3 Alkaloids In Soup Flavor Seasoning By Lc-Ms/Ms, Ma Lixing, Lu Xu, Chen Richun

Food and Machinery

In order to properly improve the integrity and reliability of the results, the LC-MS/MS method was used to determine the discrepancy of the detection results of papaverine, narcotine, thebaine in soup flavor seasoning powder. This paper based on “DB 31/2010—2012 of local standards for food safety, the Determination of Papaverine, Morphine, Narcotine, Codeine and Thebaine in Hot Pot Food by Liquid Chromatography-Tandem Mass Spectrometry”, analyzed the residue results of papaverine, narcotine, thebaine in soup flavor seasoning powder and the sources of the uncertainty of measurement. It also synthesized the total uncertainty. The results showed that the synthesis of relative standard …


Regional Discrimination Of Three Kinds Of Tea By Isotope Ratio Analysis, ZHANG Jianhui, WANG Xiali, HU Yonghui, FANG Xuanqi, DAI Xuan, ZHANG Li, LIU Lansong 2017 Hunan Institute of Food Quality Supervision Inspection and Research, Changsha, Hunan 410111, China

Regional Discrimination Of Three Kinds Of Tea By Isotope Ratio Analysis, Zhang Jianhui, Wang Xiali, Hu Yonghui, Fang Xuanqi, Dai Xuan, Zhang Li, Liu Lansong

Food and Machinery

The isotope ratio of five kinds of heavy metal elements in tea samples from different areas was measured, and the variance, cluster and discriminant analyses were undertaken using SAS (statistical analysis system) software. The results showed that 206Pb/207Pb, 66Zn/67Zn, 68Zn/67Zn, 114Cd/110Cd, 112Cd/110Cd, 26Mg/25Mg 6 indexes existed significant differences in the three regions (99% significant level). The cluster analysis results of the six indexes showed that the clustering accuracy of samples form three regions as Guzhang (78.26%), Shimen (94.44%) and Anhua (92.10%). The …


Legislative Defects And Perfection Offood Safety Crime For Food Operators, HAN Jin, YANG Tongtong, HAN Ying 2017 Harbin Engineering University, Harbin, Heilongjiang 150001, China

Legislative Defects And Perfection Offood Safety Crime For Food Operators, Han Jin, Yang Tongtong, Han Ying

Food and Machinery

Operators of food safety crime is the main target of the current field of food safety criminal legislation. From a legal perspective, the relevant provisions did not fully reflect the reality that food safety crime is becoming popular, and the harm to public safety is getting serious. Which has a series defects, such as narrowing the subjects, lighting sectencing, and too rigid fine punishment, etc. As the operators’ food safety crime has evolved into a endangering public safety crime, it is necessity to take measures, such as expansion of the food safety criminal regulation scope, more strict punishment, and adopt …


Study On Temperature And Humidity Control Ability Of Heating Type Heat Pump Drying System, WU Yaosen, GONG Li, LIU Qinghua, LIU Jun, LONG Chengshu 2017 Guangdong Institute of Modern Agricultural Equipment, Guangzhou, Guangdong 510630, China

Study On Temperature And Humidity Control Ability Of Heating Type Heat Pump Drying System, Wu Yaosen, Gong Li, Liu Qinghua, Liu Jun, Long Chengshu

Food and Machinery

In this paper, the heat and moisture balance of heating type heat pump drying system (HHPDS) is analyzed from the point of view of air conditioning. The ability that to effectively control the drying temperature and humidity is defined as the control capability. The relationship between the control capability and the drying temperature and humidity, the temperature and humidity of the environment and the amount of material is explained based on the principle of heat pump. Through the qualitative analysis of the influence of various factors, the conditions of determining the control ability of the HHPDS were obtained and it …


Design Of Semi-Automatic Pellet Racking Machine, ZHANG Yaling, ZHU Wangchun, CHENG Hao 2017 Guilin University of Electronic Science and Technology, Guilin, Guangxi 451000, China

Design Of Semi-Automatic Pellet Racking Machine, Zhang Yaling, Zhu Wangchun, Cheng Hao

Food and Machinery

Aiming at overcoming the shortcomings of the existing material dispensing technology, a semi-automatic pellet racking machine was designed, which multiply controlled the entire packaging process by the controlling system. Combining the bilateral asymmetric oscillating structure with the conveyor belt powered by the stepper motor, the racking machine distributed the pellets apart by taking two steps. The infrared count structure and aggregate silo were used to make the pellets discharged in accordance with a certain number of packaging, while the total number of pellets and the number of times were recorded. The device was concise, ingenious and could ensure the accuracy …


Design Of 3d Sugar Painting Creative Product Printing Based On Fdm, YANG Jianwei, HU Yanan, WANG Yi, ZHANG Hongxing 2017 Xi Jing University, Xi’an, Shaanxi 710021, China

Design Of 3d Sugar Painting Creative Product Printing Based On Fdm, Yang Jianwei, Hu Yanan, Wang Yi, Zhang Hongxing

Food and Machinery

The 3D sugar painting printer based on FDM technology was designed. It included the mechanical mechanism, control system and HMI interface. The mechanical mechanism consisted of the feeding mixing device and the Core XY axis linkage system. The feed mixing mechanism could accurately feed and stir the raw materials. The control system was based on CPU1214C, controlling the system motion, temperature, and stirring feeding. Siemens OP73/Micro touch screen was used to install WinCC Flexible as development platform, helping to realize human-computer interaction. This equipment could be used to print out any complex structure and delicious 3D sugar painting.


Preparation And Antimicrobial Activities Of Spi/Clove Oil Microcapsule Films, DONG Zeng, CAO Wengen, WANG Haichao, JIA Guiqi, ZHANG Xingtao, GAO Guizhen 2017 School of Biotechnology and Food Engineering, Suzhou University, Suzhou, Anhui 234000, China

Preparation And Antimicrobial Activities Of Spi/Clove Oil Microcapsule Films, Dong Zeng, Cao Wengen, Wang Haichao, Jia Guiqi, Zhang Xingtao, Gao Guizhen

Food and Machinery

Clove oil was loaded into β-Cyclodextrin (β-CD) using the saturated aqueous solution method. Antibacterial film was prepared by adding the microcapsule into Soy Protein Isolate (SPI) film, microcapsule embedding ratio, the mechanical properties of films and the bacteriostatic action to Bacillus subtilis and Escherichia coli were determined. The results showed that the embedding ratio was (67.42±3.82)%; the effect of bacteriostatic action to Bacillus subtilis and Escherichia coli of film adding more microcapsule was better,but the mechanical properties were not very well.


Expression Of Recombinant Antifreeze Peptide In Lactococcus Lactis And Its Antifreeze Activity, ZHANG Li, HUANG Xinyang, MA Tianyi, WU Jinhong, WANG Shaoyun, WANG Zhengwu 2017 School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China

Expression Of Recombinant Antifreeze Peptide In Lactococcus Lactis And Its Antifreeze Activity, Zhang Li, Huang Xinyang, Ma Tianyi, Wu Jinhong, Wang Shaoyun, Wang Zhengwu

Food and Machinery

In order to optimize the expression of antifreeze protein SF-P, using Nisin as the inducer to induced the expression of recombinant Lactococcus lactis. The expression conditions of induction time, induced pH, induced temperature and inducer Nisin concentration were optimized. The optimal expression conditions were determined by SDS-PAGE and Western blot. Furthermore, the physiological characteristics of recombinant bacteria under freezing stress were investigated by comparing the growth status, the acid production of bacteria and the changes of sodium and potassium contents before and after freezing stress. The results showed that the optimal expression conditions were determined: pH of 7.0, Nisin concentration …


Effect Of Extraction Process On Quality And Volatile Flavor Components Of Oil From Macadamia Nuts, SHUAI Xixiang, DU Liqing, ZHANG Ming, TU Xinghao 2017 South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524091, China

Effect Of Extraction Process On Quality And Volatile Flavor Components Of Oil From Macadamia Nuts, Shuai Xixiang, Du Liqing, Zhang Ming, Tu Xinghao

Food and Machinery

The effects of two extraction methods (pressing extraction, aqueous extraction) on the quality and volatile flavor compounds from macadamia nuts oil were investigated. The investigation focused on the composition of triglyceride, sterol, fatty acid and volatile flavor components, as well as the contents of tocopherols and mineral elements in macadamia nuts oil. For the fatty acid composition, triglyceride composition, and contents of tocopherols, the results showed that there were not significantly variations in pressing extracted oil and aqueous extracted oil. Fatty acid composition analysis results illustrated that the main fatty acid consisted of oleic acid, palm acid and palmitic acid, …


Optimization Of Extraction Technology Of Protein From Momordica Charantia Residue And Its Inhibitory Effect On Proliferation Of K562 Cells In Vitro, LI Meijuan, HAO Jinghao, MA Tiantian, SHI Guoqing, ZHANG Xinwu, HUANG Jihong 2017 Henan Province Food Industry Research Institute Co., Ltd,. Zhengzhou, Henan 450053, China;The State Light Industry Food Quality Supervision and Detection Station, Zhengzhou City, Zhengzhou, Henan 450053, China

Optimization Of Extraction Technology Of Protein From Momordica Charantia Residue And Its Inhibitory Effect On Proliferation Of K562 Cells In Vitro, Li Meijuan, Hao Jinghao, Ma Tiantian, Shi Guoqing, Zhang Xinwu, Huang Jihong

Food and Machinery

Single factor combined with uniform design were used to optimized the extraction process of protein from Momordica charantia residue by salt dissolving method, and the inhibitory activity in vitroon K562 cell proliferation of crude extract was determined by MTT assay. The test results showed that the optimal conditions were as followed: salt concentration of 0.25 mol/L, time 3.59 h, pH=9, ratio of liquid-material 10.36︰1 (mL/g) and the temperature of 25 ℃, extracting twice. Under this condition, the actual extraction rate was 9.28%. In the range of experimental concentration, the crude protein from Momordica charantia residue had a significant inhibitory effect …


Effect Of Different Extraction Methods On The Properties And Biological Activity Of Polysaccharides From Radix Glehniae, JING Yongshuai, ZHANG Danshen, ZHANG Ruijuan, SU Lei, PAN Mengshu, SUN Shuaishuai, WU Lanfang, ZHENG Yuguang 2017 College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China

Effect Of Different Extraction Methods On The Properties And Biological Activity Of Polysaccharides From Radix Glehniae, Jing Yongshuai, Zhang Danshen, Zhang Ruijuan, Su Lei, Pan Mengshu, Sun Shuaishuai, Wu Lanfang, Zheng Yuguang

Food and Machinery

The polysaccharides were extracted from Radix Glehniae, by using hot water extraction, ultrasonic extraction, microwave extraction, lye extraction, acid extraction and cellulase extraction, and the corresponding polysaccharide was GLP-W, GLP-U, GLP-M, GLP-L, GLP-A and GLP-E. The extraction rate, soluble sugar content, viscosity, structural characteristics, solid morphology, antioxidant activity and hypoglycemic activity of each polysaccharide were measured. The results showed: the extraction rates of polysacchar-ides were GLP-A(39.62%)>GLP-E(38.33%)>GLP-L(24.80%)>GLP-W(23.93%)>GLP-M(18.92%)>GLP-U(12.68%); The viscosity of the polysaccharide solution by different extraction methods were increased with the increase of polysaccharide solution concentration, and at the same concentration, GLP-L had the largest viscosity. The content of soluble sugar …


Extraction Optimization Of Volatile Oil From Chinese Elder Herb By Supercritical Co2 And Its Composition Analysis, JIANG Hongyu, LIU Yufeng, XIE Guofei, LIU Yilin, LUO Xiaofang, WANG Zongcheng 2017 Hunan Provincial Engineering Research Center for Ginkgo Biloba, Hunan University of Science and Engineering, Yongzhou, Hunan 425199, China

Extraction Optimization Of Volatile Oil From Chinese Elder Herb By Supercritical Co2 And Its Composition Analysis, Jiang Hongyu, Liu Yufeng, Xie Guofei, Liu Yilin, Luo Xiaofang, Wang Zongcheng

Food and Machinery

The extraction conditions of volatile oil from Chinese elder herb by supercritical CO2 were optimized by single factor experiment and response surface methodology. Flow rate of CO2, the pressure, temperature and time of extraction were employed to examine the influences of the yield of volatile oil. The chemical constituents were separated and identified by Gas chromatography-mass spectrometry (GC-MS). The optimal extracting conditions were flow rate of CO2 25 L/h, extraction pressure 30.5 MPa, at 45.0 ℃ for 2.8 h. The extraction rate of volatile oil from Chinese elder herb was 0.86%. 16 kinds of volatile compounds …


Key Technology On The Preparation Of Full Bean Milk By Minuteness Wet Pulverization Method, YANG Xinjun, CUI Zhengwei, ZHANG Yuzhong, DAI Ning 2017 College of Mechanical Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China; Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment & Technology, Wuxi, Jiangsu 214122, China

Key Technology On The Preparation Of Full Bean Milk By Minuteness Wet Pulverization Method, Yang Xinjun, Cui Zhengwei, Zhang Yuzhong, Dai Ning

Food and Machinery

To investigate the application of minuteness wet pulverization method on the preparation of full bean milk, experimental and numerical analysis were applied in this paper. The influences of different technological parameters on the fiber grain size after the pulverization were also studied, and the empirical formula was obtained by dimensional analysis. The results showed that the average volume size of fiber grain decreased with the increase of cycle number, static blade number and rotating speed. However, the cut depth determined the limitation size of fiber grain. The increase of cycle number would not affect the grain size after the pulverization …


Preparation Of Iron-Chelating Peptides From Zanthoxylum Bungeanum Seed Protein Using Enzymatic Hydrolysis, YUAN Hong, CHAI Liqin, QUAN Li, WANG Lixia, TIAN Chengrui, WANG Xiaoyu 2017 College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an, Shaanxi 710119, China

Preparation Of Iron-Chelating Peptides From Zanthoxylum Bungeanum Seed Protein Using Enzymatic Hydrolysis, Yuan Hong, Chai Liqin, Quan Li, Wang Lixia, Tian Chengrui, Wang Xiaoyu

Food and Machinery

Zanthoxylum bungeanum seed protein was hydrolyzed with different proteases, and the optimal protease was screened based on the hydrolysis degree and iron-chelating ability of hydrolysates, then iron-chelating peptides were prepared by the optimal protease. Single factor method and response surface methodology based on Box-Behnken design using four factors and three levels were carried out to optimize the conditions of Zanthoxylum bungeanum seed iron-chelating peptide, and the effects, including substrate concentration, enzyme/substrate ratio, hydrolysis pH, temperature, time on iron-chelating ability of hydrolysate were studied. The results indicated alcalase proteases was the optimal protease to prepare iron-chelating peptides from Zanthoxylum bungeanum seed …


Quality Changes Of Yak Meat Under Different Heat Medium And Time, LI Shengsheng, JIN Yichao 2017 Academy of Animal and Veterinary Sciences, Qinghai University, Xining, Qinghai 810016, China; Qinghai Academy of Animal and Veterinary Sciences, Xining, Qinghai 810016, China

Quality Changes Of Yak Meat Under Different Heat Medium And Time, Li Shengsheng, Jin Yichao

Food and Machinery

Cooking loss, heat shrinkage rate, color, shear force, and texture of yak meat were measured, which were heated by water and steam at 10, 20, 30, 40, 50, 60 min, respectively, and the correlation of heat time and quality were analyzed. The results showed that cooking loss of yak meat significantly increased with the extension of heat time (P<0.05), and the increased rate of cooking loss heated by water 21.21% higher than by steam. The heat shrinkage rate of yak meat by water bigger than by steam after being heated from 10 min to 40 min, and the heat shrinkage …


Designing Model For Cigarette Weight Based On Filling Power Of Cut Tobacco, XIANG Hu, HE Xiaoqiang, LIU Geyi, LI Shaochen, YANG Tao, WANG Long, GUO Ge 2017 Qujing Cigarette Factory, HongyunHonghe Tobacco ﹝Group﹞ Co., Ltd., Qujing, Yunnan 655001, China

Designing Model For Cigarette Weight Based On Filling Power Of Cut Tobacco, Xiang Hu, He Xiaoqiang, Liu Geyi, Li Shaochen, Yang Tao, Wang Long, Guo Ge

Food and Machinery

To design a suitable cigarettes weight, on the basis of filling power of cut tobacco, build designing model for cigarette unit weight considertng the specification of cigarette, cigarette paper, tipping paper, ecreteur and the weight of filter rod. The examples indicated that the hardness of the cigarette whose weight designed by the modelis suitable.By evaluation, the sensory quality of the cigarette meets the design requirements, and nothing quality defects such as loose-end and fall of burning cone appeared during the process of evaluating. The unit cigarette weight designed by the model could not only guarantee the physical index and sensory …


Digital Commons powered by bepress