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Concentration Of Listeria Monocytogenes In Skim Milk And Soft Cheese Through Microplate Immunocapture, Steven A. Rogers, Melissa Calicchia, Rosalee S. Hellberg 2018 Chapman University

Concentration Of Listeria Monocytogenes In Skim Milk And Soft Cheese Through Microplate Immunocapture, Steven A. Rogers, Melissa Calicchia, Rosalee S. Hellberg

Food Science Faculty Articles and Research

Microplate immunocapture is an inexpensive method for the concentration of foodborne pathogens using an antibody-coated microplate. The objective of this study was to determine the efficacy of microplate immunocapture as an alternative to traditional enrichment for concentrating Listeria monocytogenes to levels detectable with selective plating or real-time PCR. L. monocytogenes isolates serologically characterized as Type 1 (1/2a) and Type 4 (untypeable) were grown overnight and diluted to 100 to 106 colony-forming units (CFU)/mL. The isolates were used to optimize microplate immunocapture in tryptic soy broth with 0.6% yeast extract (TSBYE), skim milk, and queso fresco samples. Following …


Effects Of Ph And Heat Treatments On Structure And Functional Properties Of Rice Proteins, LIU Fang, XU Zhou, CHEN Maolong, CHENG Yunhui 2018 College of Chemical and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China

Effects Of Ph And Heat Treatments On Structure And Functional Properties Of Rice Proteins, Liu Fang, Xu Zhou, Chen Maolong, Cheng Yunhui

Food and Machinery

In this study, rice protein was used as raw material to study the effect of pH treatment and heat treatment on the structure and functional properties of rice protein. Studies have found that the functional properties of rice proteins have been improved to some extent after being treated with different pH values for 40 minutes. The solubility, emulsifying and emulsifying stability, foaming property and surface hydrophobicity of RP treated at pH 12.0/40 min were 5.16%, 0.626% and 12.64%, and 135%, respectively, with 2.46, 2.85, 1.12, and 2.51 times higher than that of the RP. At this time, the fraction of …


Experimental Study On The Expression Level Of Opn And Tgf-Β1 In Rat Liver Fibrosis Model With Tuber Granule Intervention, YUAN Yong 2018 Hainan Medical College, Haikou, Hainan 570102, China

Experimental Study On The Expression Level Of Opn And Tgf-Β1 In Rat Liver Fibrosis Model With Tuber Granule Intervention, Yuan Yong

Food and Machinery

Methods The 30 rats were randomly divided into normal control group, the non-treatment group of liver fibrosis model and the treatment group of liver fibrosis model. Each group of rats are take the following operation: Western blotting test OPN protein expression after eitht weeks。The PCR detection of TGF-β1 mRNA expression level. Results This study showed that the treatment group of liver fibrosis model of HA, LN, PCIII, CIV radioimmunoassay were higher than the treatment group have significant differences (P<0.01), and the differences were statistically significant than the non-treatment group of liver fibrosis model at the same time(P<0.05). The non-treatment group …


Locally Sonochemical Effect On Total Phenolic Contents And Theirantioxidant Abilities In The Extracts From Sweet Orange Peels, ZHANG Zheng, MA Yaqin, WANG Pengxu, CHENG Chuanxiang, ZHU Panpan 2018 Citrus Research Institute, Southwest University, Chongqing 400712, China; National Citrus Engineering Research Center, Chongqing 400712, China

Locally Sonochemical Effect On Total Phenolic Contents And Theirantioxidant Abilities In The Extracts From Sweet Orange Peels, Zhang Zheng, Ma Yaqin, Wang Pengxu, Cheng Chuanxiang, Zhu Panpan

Food and Machinery

The effect of ultrasonic parameters including ultrasonic treatment temperature and time and the ultrasonic treatment power on the content and antioxidant capacity of phenolic acids from orange leaves peels in a local position were explored. The results indicated that ferulic acid content was the highest in orange leaves and peels, and both the content of seven phenolic acids and total phenolic contents (TPC) were increased with the increase of treatment time and temperature with some small differences. 30 ℃ was found to be the optimal temperature. Ultrasonic treatment power was also revealed to have a positive effect on the content …


Study On The Effect Of Maillard Reaction On Antioxidant Activity Of Sesame Polypeptides, QIAN Senhe, WANG Zhou, WEI Ming, XUE Zhenglian 2018 Department of Biochemistry, Anhui Polytechnic University, Wuhu, Anhui 241000, China; Research Institute of Microbial Pharmaceutical Industry, Anhui Polytechnic University, Wuhu, Anhui 241000, China

Study On The Effect Of Maillard Reaction On Antioxidant Activity Of Sesame Polypeptides, Qian Senhe, Wang Zhou, Wei Ming, Xue Zhenglian

Food and Machinery

Based on the preparation of sesame peptides, the effect of Maillard reaction on antioxidant activity of sesame polypeptides was studied. The results showed that the suitable ratio of peptide to sugar was 1∶2 for Maillard reaction of sesame polypeptide. The Maillard reaction could significantly improve the antioxidant activity of sesame polypeptide, and the reducing power, ABTS+ radical scavenging rate, DPPH radical scavenging rate and hydroxyl radical scavenging rate were increased by 121.4%, 304.5%, 81.2% and 103.2%, respectively. The contents of lysine methionine and tyrosine were reduced, but the contents of alanine and valine were increased, moreover, aspartic acid, glutamic …


Effect Of Salt Content On Physicochemical Properties And Antioxidant Activity During Post-Fermentation Of Douche, WAN Jun, CHENG Weiwei, SHEN Xiaolu, JIANG Aimin 2018 Guangdong AIB Polytechnic College, Guangzhou, Guangdong 510507, China

Effect Of Salt Content On Physicochemical Properties And Antioxidant Activity During Post-Fermentation Of Douche, Wan Jun, Cheng Weiwei, Shen Xiaolu, Jiang Aimin

Food and Machinery

The impact of salt content on post-fermentation of douchi was studied. In the post-fermentation stage, 8%, 10%, 12% of salt were added for 30 days fermentation. The results showed that, with the increase of salinity, the total acids and pH decreased, the hardness and chewiness increased, which shallowed the color and declined the taste of douchi. The high salinity was bad for producing the total amino acid nitrogen and γ-PGA, and also inhibited the antioxidant activity of douchi. Finally, 8% of salt was considered to be the best, but the flavor remained to be improved.


Study On Enzymatic Hydrolysis-Mild Thermal Oxidation Of Chicken Fat For Preparing Chicken Flavor, YU Jingyang, CHEN Huangnv, XIA Shuqin, ZHANG Xiaoming 2018 School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China

Study On Enzymatic Hydrolysis-Mild Thermal Oxidation Of Chicken Fat For Preparing Chicken Flavor, Yu Jingyang, Chen Huangnv, Xia Shuqin, Zhang Xiaoming

Food and Machinery

At present, commercial refined chicken fat and chicken protein hydrolysates are usually used as raw materials for the industrialized production of Maillard reaction chicken flavor. However, commercial refined chicken fat contains a large amount of anti-oxidant, which is hard to be oxidized by low-temperature heating. It is easy to produce off-flavor for chicken fat when heated at high temperature. In order to form enough chicken flavor precursors for Maillard reaction chicken flavor preparing, we studied the mild thermal oxidation of refined chicken fat based on enzymatic hydrolysis, which was with the lipolytic ratio, fatty and greasy taste as evaluation indexes. …


Preparation, Characterization And Sustained-Release Property Of Na2Seo3-Loading Chitosan Microsphere, ZHANG Chi, SHANG Longchen, WU Shaowei, LI Guangda, LIU Xinping 2018 College of Biological Science and Technology, Hubei Minzu University, Enshi, Hubei 445000, China

Preparation, Characterization And Sustained-Release Property Of Na2Seo3-Loading Chitosan Microsphere, Zhang Chi, Shang Longchen, Wu Shaowei, Li Guangda, Liu Xinping

Food and Machinery

Chitosan was chosen as wall material to prepare the Na2SeO3-loading microspheres with emulsion-crosslink method. Then, the microspheres’ appearance, drug loading capacity, encapsulation efficiency and its sustained-release property were chosen as evaluation criteria to optimize the process condition through the single factor experiments and the orthogonal tests. In addition to this, the structure of the microspheres was characterized preliminarily by the scanning electron microscopy (SEM), infrared spectroscopy (IR), atomic fluorescence, thermogravimetric analysis (TG), and its sustained-release property were researched in vitro under the circumstance of the simulated body fluid (SBF) which was prepared by ourselves. The best process condition …


Study On Volatile Flavor Compounds Of Meat From Yanbian Yellow Cattle, HOU Tingting, NAN Jingxi, LIN Mo, XU Meina, LIU Tian, WANG Juan, LI Guanhao 2018 Agricultural College of Yanbian University, Yanji, Jilin 133000, China; Food Research Center of Yanbian University, Yanji, Jilin 133000, China

Study On Volatile Flavor Compounds Of Meat From Yanbian Yellow Cattle, Hou Tingting, Nan Jingxi, Lin Mo, Xu Meina, Liu Tian, Wang Juan, Li Guanhao

Food and Machinery

Volatile flavor compounds in three parts of meat from Yanbian yellow cattle were analyzed by headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). The extraction fibers were optimized by comparison of 65 μm PDMS/DVB, 75 μm CAR/PDMS and 100 μm PDMS. The results showed that 65 μm PDMS/DVB had the best extraction effect on the volatile components of meat from Yanbian yellow cattle. Furthermore, the volatile components in Longissimus dorsi, rump and Sirloin of Yanbian Yellow Cattle were respectively investigated, and the differences of the volatile components in the three parts were analyzed with principal component analysis. The …


Gc/Ms For Simultaneous Determination Of 14 Photo-Initiators In Fatty Food, XU Wenyang, CHEN Hua 2018 Hunan Research Institute of Food Quality Supervision and Inspection, Changsha, Hunan 410017, China

Gc/Ms For Simultaneous Determination Of 14 Photo-Initiators In Fatty Food, Xu Wenyang, Chen Hua

Food and Machinery

To establish a GC-MS method for determination of 14 photo-initiators in fatty food. The homogeneous samples were extracted by acetone-hexane(2∶8,v:v) for 15 min at room temperature. The supernatant was purified by HLB column, and the eluent was then collected, which was further purified by PSA. The purified eluent was centrifuged and determined by GC-MS. The recoveries were between 73.0%~92.4% at spiked levels of 0.000 2~0.001 0 mg/kg, and the relative standard deviation of the methods were 4.1%~8.9%, with the LOD ranging from 0.02~0.20 μg/kg. It was in accordance with the requirement of technical parameters for analysis with fast and simple …


Watermelon Sugar Content Detection Based On The External Characteristics And Sub-Band Spectrum Centroid Method, ZHUANG Wei, LI Zhenfeng, SONG Feihu, LI Jing, ZHU Guanyu 2018 School of Mechanical Engineering of Jiangnan University, Wuxi, Jiangsu 214222, China; Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment and Technology, Wuxi, Jiangsu 214222, China

Watermelon Sugar Content Detection Based On The External Characteristics And Sub-Band Spectrum Centroid Method, Zhuang Wei, Li Zhenfeng, Song Feihu, Li Jing, Zhu Guanyu

Food and Machinery

At present, the vibration nondestructive testing method has high requirements on hardware system and test conditions, and few practical application systems have been designed..In order to solve this problem, a portable vibration test detection system was built. The time signal was denoised by the method of wavelet threshold denoising. The sub-band spectrum centroid of a certain watermelon variety was extracted. The product of square of transverse diameter and the longitudinal diameter was proposed as the volume parameters. It is found that the frequency point of sub-band spectrum centroid is negatively correlated with the quality of watermelon and the volume parameters, …


Detection Models Of Mildew Degree In Honeysuckle Based On Hyperspectral Imaging Technology, FENG Jie, LIU Yunhong, WANG Qingqing, YU Huichun, SHI Xiaowei 2018 College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan 471023, China

Detection Models Of Mildew Degree In Honeysuckle Based On Hyperspectral Imaging Technology, Feng Jie, Liu Yunhong, Wang Qingqing, Yu Huichun, Shi Xiaowei

Food and Machinery

Hyperspectral imaging technology was applied to develop a rapid, accurate and non-destructive detection method for honeysuckle mildew degree levels. The original spectral data were analyzed by three pretreatment methods with Savitzky-Golay (SG) convolution smoothing, Multiple Scatter Correct (MSC) and SG-MSC. A comparison was made among SG, MSC and SG-MSC based on Partial Least Squares (PLS), of which the best pretreatment method was SG-MSC. The Successive Projection Algorithm (SPA) and Competitive Adaptive Reweighted Sampling (CARS) were used to extract the characteristic wavelengths after SG-MSC pretreatment. Partial Least Square Discriminant Analysis (PLS-DA) and Last Squares Support Vector Machine (LS-SVM) were applied to …


Analysis Of Monosaccharide Composition And Content Of Polysaccharide From Nostoc Sphaeroides Kützing Extractive, ZHOU Xi, WANG Xi, LIU Shuiping, LUO Cheng, WANG Zongcheng, ZHUANG Ruihua 2018 Zhuzhou Institute for Food and Drug Control, Zhuzhou,Hunan 412000, China

Analysis Of Monosaccharide Composition And Content Of Polysaccharide From Nostoc Sphaeroides Kützing Extractive, Zhou Xi, Wang Xi, Liu Shuiping, Luo Cheng, Wang Zongcheng, Zhuang Ruihua

Food and Machinery

A simple and sensitive high performance liquid chromatographic method for simultaneous determination of eight kinds of neutral monosaccharides, two kinds of uronic acid and one kinds of glycosamine by precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) has been developed. The polysaccharides hydrolyzed effects of hydrolysis time, temperature and trifluoroacetic acid (TFA) concentration were investigated. HPLC were established for analyzing the monosaccharide composition and the content of polysaccharide from Nostoc sphaeroides Kützing extractive. The results showed that the recoveries of nine kinds of monosaccharides, such as Man, GlcUA, Glc, Xyl, were 81.08%~102.1%. Compared with the chemical method. The established method is accurate, goodlineal …


Study On The Removal Of Methylene Blue Using Rice Starch As An Adsorbent By Mini-Hydrocyclone, WU Han, YU Jianfeng, JIANG Yuting, HE Shiyuan, ZHAO Jiang 2018 School of Mechanical Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China; Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment & Technology, Wuxi, Jiangsu 214122, China

Study On The Removal Of Methylene Blue Using Rice Starch As An Adsorbent By Mini-Hydrocyclone, Wu Han, Yu Jianfeng, Jiang Yuting, He Shiyuan, Zhao Jiang

Food and Machinery

Rice starch was used as an adsorbent to adsorb the dyeing wastewater simulated by methylene blue in mini-hydrocyclone. The single experiments were used to explore the effect of starch concentration, split ratio and feed flow rate on the efficiency of adsorption and separation. Moreover, the response surface analysis method was used to optimize process parameters. The results showed that the starch concentration, split ratio and feed flow rate all influenced the efficiency of adsorption and separation. The order of effect on adsorption efficiency was as followed: starch concentration > split ratio > feed flow rate, and the order of effect on separation …


Design And Application Of Round Fruit Sorting And Labeling Machine, SUN Kai, YAN Xuehui, ZHU Rongsheng 2018 College of Engineering, Northeast Agricultural University, Harbin, Heilongjiang 150030, China

Design And Application Of Round Fruit Sorting And Labeling Machine, Sun Kai, Yan Xuehui, Zhu Rongsheng

Food and Machinery

At present, the sorting and labeling of round fruits in peasant household is still depending on the manpower with low efficiency. This paper presents a solution for the integration of sorting and labeling. An automatic sorting and labelling machine is designed for selecting and labeling the size of round fruit in peasant household. This machine is mainly composed of roller type sorting device and automatic labelling device, which can automatically complete the sorting and labeling of round fruit. The optimal parameters of each part were determined through the elaboration of the whole structure and the working principle of each part …


Evaluation Of Food Filling Machine Design Scheme Based On Interval Analytic Hierarchy Process And Membership Degree, SHI Hongyan, ZHENG Shuxian 2018 College of Mechanical Engineering, Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolla 028043, China

Evaluation Of Food Filling Machine Design Scheme Based On Interval Analytic Hierarchy Process And Membership Degree, Shi Hongyan, Zheng Shuxian

Food and Machinery

In view of the uncertainty in the evaluation of the design of food filling machine, a new evaluation method for the design of food filling machine was proposed. Firstly, the evaluation index system of the design scheme of the food filling machine was set up, and the grade of the design scheme evaluation and the interval number of the food filling machine were put forward. Moreover, the interval number weight of the evaluation index of the design scheme of the food filling machine was determined by the interval analytic hierarchy process. Furthermore, an evaluation model of food filling machine design …


Frictional Resistance Analysis And Parameter Optimization Of Extruder Screw Based On The Principle Of Continuum Medium Hypothesis, MAO Jun, LU Nan, XIE Miao, LU Jinnan, JIA Kai 2018 School of Mechanical Engineering, Liaoning Technical University, Fuxin, Liaoning 123000, China

Frictional Resistance Analysis And Parameter Optimization Of Extruder Screw Based On The Principle Of Continuum Medium Hypothesis, Mao Jun, Lu Nan, Xie Miao, Lu Jinnan, Jia Kai

Food and Machinery

Based on the hypothesis of continuous medium in theoretical mechanics, the friction resistance model of screw rotary extrusion material was established. The influence of multiple structural parameters of screw on friction resistance was analyzed by using this model, and the range of parameter selection was also given. The friction force between screw and material was the target, and the screw structure parameter was the variable, using Matlab optimization toolbox to optimize the design of screw structure parameters. Moreover, the friction force of the material flow in the optimized screw was analyzed by Ansys. The results showed that the friction stress …


Studies On Α-Lipoic Acid Molecularly Imprinted Membrane And Its Antioxidant Properties, YANG Bowen, CHEN Yuqin, ZHU Qiujin 2018 School of Liquor and Food Engineering, Guizhou University, Guiyang, Guizhou 550025, China

Studies On Α-Lipoic Acid Molecularly Imprinted Membrane And Its Antioxidant Properties, Yang Bowen, Chen Yuqin, Zhu Qiujin

Food and Machinery

Thermo-sensitive α-lipoic acid molecularly imprinted polymers (ALA-MIPs) were synthesized through pre-polymerization using α-lipoic acid as a functional monomer and N-vinylcaprolactam (NVCL) as both thermo-sensitive monomer and functional monomer. Poly(acrylonitrile) (PAN) and Poly(vinylidene fluoride) (PVDF) were used as the main raw material to synthesis molecularly imprinted membranes through the heterogeneous particle-filling method by adding ALA-MIPs. The morphology of the molecularly imprinted membrane(MIM) was investigated by scanning electron microscopy. The mechanical properties, hydrophilic properties, water absorption abilities, and ALA release properties of the membrane were also investigated. The results show that the PVDF membrane has better mechanical properties and hydrophobic properties when …


Design The Rural Food Packaging Design Under The Ethical Concept, ZHANG Jiaxin, ZHU Li 2018 School of Architecture and Art, Central South University, Changsha, Hunan 410083, China

Design The Rural Food Packaging Design Under The Ethical Concept, Zhang Jiaxin, Zhu Li

Food and Machinery

Food packaging in the countryside deeply reflects a kind of intimate complex relationship between human and design, human and tradition, human and natue. We incorporate design ethics with the process of food packaging design, as to make people to think about this comprehensive relationship from the perspective of ethics and introduce ethical care into design practice from top to bottom, and finally we can establish clear design ethics awareness for future country food packaging.


Preparation Of Eggshell-Powder Nanosilver And Its Antibacterial Properties, CHEN Qiaoling, CHEN Bisang, WU Xiuting, YOU Yuting 2018 College of Biological Science And Biotechnology, Minnan Normal University, Zhangzhou, Fujian 363000, China

Preparation Of Eggshell-Powder Nanosilver And Its Antibacterial Properties, Chen Qiaoling, Chen Bisang, Wu Xiuting, You Yuting

Food and Machinery

Eggshell-powder as reactant react with AgNO3 and NaBH4 through ultrasonic reduction method to prepare the eggshell-powder nanosilver. The eggshell-powder nanosilver morphology and morphology changes of treated bacteria were characterized by using Scanning Electron Microscope (SEM). And its antibacterial properties were characterized by the microorganism meathods. The results showed that silver nanoparticles of eggshell powder had strong bactericidal effect on Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Candida albicans etc. And the minimum inhibitory concentration (MIC) of this four bacterias were 1.5,3.0,3.0,6.0 mg/L and minimum bactericidal concentration (MBC) were 6, 12, 12, 24 mg/L.


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