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Development Of A Loop-Mediated Isothermal Amplification Assay For Visual Detection Of Staphylococcus Aureus, SONG Taoping, QIU Huali, WANG Shujuan, PENG Xinkai, YANG Lixia 2015 Changsha Centre of Supervision & Inspection on Food Quality Safety, Changsha, Hunan 410013, China

Development Of A Loop-Mediated Isothermal Amplification Assay For Visual Detection Of Staphylococcus Aureus, Song Taoping, Qiu Huali, Wang Shujuan, Peng Xinkai, Yang Lixia

Food and Machinery

To develop a loop-mediated isothermal amplification (LAMP) assay for visual detecting Staphylococcus aureus, 2 sets of specific primers were designed and 6 primers targeting the nuc gene was selected. The method could detect Staphylococcus aureus within 45 min at a constant temperature of 63 ℃. The sensitivity of the method was 1 pg/μL, and had no cross reaction with other pathogens. The result indicated that the LAMP assay was a rapid, specific and simple tool for field diagnosis of Staphylococcus aureus.


Research On Degenerate Primers For Detection Of Staphylococcus Aureus Enterotoxin A, B Gene, LIU Jichao, CHEN Bairu, JIANG Tiemin, CHEN Lijun 2015 Beijing Sanyuan Foods Co.Ltd., Beijing 100163, China

Research On Degenerate Primers For Detection Of Staphylococcus Aureus Enterotoxin A, B Gene, Liu Jichao, Chen Bairu, Jiang Tiemin, Chen Lijun

Food and Machinery

The degenerate primers were designed to establish a PCR assay for synchronous and fast detection of staphylococcal enterotoxin A and B. According to the sequences of SEA and SEB gene, primers were designed and used for PCR. The lengths of the amplified primers were 105 bp and 135 bp. To evaluate the specificity of PCR assay, 2 strains of SEA, SEB and 4 strains of non-SEA-B were tested by PCR. SEB was 10-fold serially diluted and amplified by PCR to verify its sensitivity. The results showed that the detection of Staphylococcus aureus enterotoxin A, B strain DNA results were positive …


Determination Of Mercury Content In The Food Paper-Plastic Composite Packaging Materials, PENG Xianglian, FU Hongjun 2015 College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; National Engineering Laboratory for Rice and Byproducts Processing, Changsha, Hunan 410004, China

Determination Of Mercury Content In The Food Paper-Plastic Composite Packaging Materials, Peng Xianglian, Fu Hongjun

Food and Machinery

A method for determining mercury content in the food paper-plastic composite packaging materials with wet digestion-atomic fluorescence spectrometry was developed. The sample of 0.500 0 g with 10 mL of concentrated nitric acid and 2 mL of hydrogen peroxide digestion 4 h could get the best results. The best optimized atomic fluorescence spectrometer detection conditions of mercury were as followed: a negative high voltage of 210 V, the shielding gas flow rate of 900 mL/min, the carrier gas flow rate of 500 mL/min, injection volume of 0.5 mL, the Hg lamp current of 10 mA, the atomizer height of 10 …


Detection Of Methimazole Residue In Animal-Derived Foods By Combining Solid-Phase Extraction With High Performance Liquid Chromatography, CHI Yonghong, YUN Yaguang 2015 Baotou Light Industry Vocational Technical College, Baotou, Inner Mongolia 014035, China

Detection Of Methimazole Residue In Animal-Derived Foods By Combining Solid-Phase Extraction With High Performance Liquid Chromatography, Chi Yonghong, Yun Yaguang

Food and Machinery

The residue of antithyroid drug - methimazole in animal-derived food products was studied. The complex matrix of food products was purified by using Cleanert S C18 solid-phase extraction column and the experimental parameters including the loading solvent, washing solution and the eluent were optimized in detail. The method for the accurate and sensitive detection of trace methimazole in pig kidney, liver and muscle samples was successfully developed by combining solid-phase extraction with high performance liquid chromatography. Under the optimized experimental conditions, good recoveries (>85%) were obtained at three spiked concentrations in chosen animal-derived samples with RSD less than …


Determination Of Vitamin C In Red Globe Grape Based On Visible/Near-Infrared Diffuse Reflectance Spectroscopy, CHEN Chen, LU Xiaoxiang, ZHANG Peng, CHEN Shaohui, LI Jiangkuo 2015 College of Biotechnology and Food Science, Tianjin University of Commerce Tianjin, Tianjin 300134, China; Key Laboratory of Food Biotechnology, Tianjin 300134, China

Determination Of Vitamin C In Red Globe Grape Based On Visible/Near-Infrared Diffuse Reflectance Spectroscopy, Chen Chen, Lu Xiaoxiang, Zhang Peng, Chen Shaohui, Li Jiangkuo

Food and Machinery

For establishing the determination model of vitamin C content of Red Globe Grape using visible / near infrared diffuse reflectance spectroscopy, and evaluating its application value, effects on scaling model results of different chemometry modeling methods, different spectra pretreatment methods and different effective wavelength intervals were discussed, and the samples in prediction set were used to verified the accuracy of the optimal model. The results showed that in the whole spectral range, application of modified partial least squares (MPLS) combined with first derivative, 5 points smoothing, weighted multivariate discrete correction (WMSC) could obtain the optimal calibration models, the cross validation …


Sampling Methods And Application Research On Rapid Measuring Instrument Of Tobacco Widthbased On Scanning Imaging, LIU Yang, LIU Minchang, WEN Ruoyu, ZHAO Weiyi, ZENG Jian, ZHOU Xuezheng 2015 China Tobacco Chuanyu Industrial Corporation Technical Research Center, Chengdu, Sichuang 610066, China

Sampling Methods And Application Research On Rapid Measuring Instrument Of Tobacco Widthbased On Scanning Imaging, Liu Yang, Liu Minchang, Wen Ruoyu, Zhao Weiyi, Zeng Jian, Zhou Xuezheng

Food and Machinery

The width of cut tobacco is an important index to evaluate sensory qualities and physical indices of tobacco products. The rapid measuring instrument of cut tobacco showshigh efficiency and accuracy. This study based on a kind of cut tobacco set up the sampling methods about the rapid measuring instrument. Meanwhile, tobacco width measuring methods by using this instrument and using the plastic rod are contrasted. Moreover, this paper had done some work of application about different tobacco cuts including conditional tobacco, expanded tobacco and tobacco flake and different types of tobacco cutters. The results show that the rapid measuring instrument …


Research On Application Of Cognitive Radio Wireless Sensor Networks In Food Production, BAO Yafu, ZHANG Shuang, SUN Hui 2015 Changchun Vocational Institute of Technology, Changchun, Jilin 130033, China

Research On Application Of Cognitive Radio Wireless Sensor Networks In Food Production, Bao Yafu, Zhang Shuang, Sun Hui

Food and Machinery

Wireless sensor networks are facilitated in detecting food security and production. The key of this system is to capture the information from sensors without error. The idea of Cognitive Radio network (CRN) is used to eliminate signal interference and channel jamming in wireless sensor networks based on RFID and simulation results in Matlab shows that the modeling and analysis are valid.


Construction Of Consumer Safety Food Trust Evaluation Mechanism Under Information Exchange, WANG Zhenduo 2015 School of Electronic & Information, College of Siyuan, Xi'an, Shaanxi 710038, China

Construction Of Consumer Safety Food Trust Evaluation Mechanism Under Information Exchange, Wang Zhenduo

Food and Machinery

One of the important reasons for the consumer's degree of trust is not high, is that consumers are hard to obtain the information generated by the corresponding safety food certification information asymmetry. This kind of information asymmetry arises because the cognitive ability to consumer overvalued, certification bodies lack of credibility, administrative organs of local protectionism, and the media reported that the distortion and food enterprise and consumer communication channels of the building neglect problem on existence. Therefore, must be taken to strengthen the publicity and education of consumers, strengthen the management and supervision of certification bodies to the establishment of …


Analysis On Mechanical Properties Of Green Walnut Based On Workbench, ZHENG Jiahong, WU Dongze, LIANG Jinsheng, NIU Shuoya 2015 College of Mechanical and Electrical Engineering, Shaanxi University of Science & Technology, Xian, Shaanxi 710021, China

Analysis On Mechanical Properties Of Green Walnut Based On Workbench, Zheng Jiahong, Wu Dongze, Liang Jinsheng, Niu Shuoya

Food and Machinery

According to the characteristics of the stress in the process of the peeling of walnut, analyzed the static characteristics of the walnut and create a geometrical model of the green walnut. By the pressure text of the green walnut under different loading direction, simulated the mechanical characteristics at the same time by using workbench. Then, by comparing the pressure test and the simulation results, two results were obtained, which proves the feasibility of the simulation analysis. In addition, the establishment of mechanical model can be also used to analyze the walnut green damage theory during the peeling process, which provided …


Analysis And Calculation Method Of Potato Sorting Conveyor Line Statics, SUN Jun, YU Wenqiang, LI Xueqiang, SHI Yuanyuan, YU Mingzhi 2015 School of Mechanical Engineering, Shandong University of Technology, Zibo, Shandong 255000, China

Analysis And Calculation Method Of Potato Sorting Conveyor Line Statics, Sun Jun, Yu Wenqiang, Li Xueqiang, Shi Yuanyuan, Yu Mingzhi

Food and Machinery

The operation, of sorting and the arrival of potatoes, is still on low mechanization degree and involves heavy laboring intensity. however, there is little research on optimizing the devices of conveyor line interiorly. Based on a reference to some foreign research results about the conveyor line with the function of sorting potatoes by sizes, this article present proper design parameters adjusted to the properties sorting and warehouse management of potatoes in domestic situation by simulating and optimizing design. It offers the reference data for the design and manufacture of domestic device for sorting and conveying the potatoes. It also helps …


Dynamics Simulation Of Food Sorting Robot, JIANG Yi, LI Zhengyang, YING Zaien, PING Xueliang 2015 School of Mechanical Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China; Jiangsu Province Key Laboratory of Advanced Food Manufacturing Equipment and Technology, Wuxi, Jiangsu 214122, China

Dynamics Simulation Of Food Sorting Robot, Jiang Yi, Li Zhengyang, Ying Zaien, Ping Xueliang

Food and Machinery

According to the structural features of food sorting robots with six degrees of freedom, kinetic theory analysis was carried out by using Lagrange approach. Virtual prototype modeling was established, and the curves of angle, angular velocity, angular acceleration, moment and power were obtained by rigid body dynamics simulation within ADAMS. The results showed that the robot had a good motion stability and dynamic response, which in addition provide a theoretical basis for motor selection. Coupled dynamics simulation was also carried out by SimDesigner, which demonstrates the rationality of the manipulate design.


Motion Simulation Analysis Of Yield Monitor Device On Tomato Harvester Based On Solidworks Motion, YUAN Changfu, ZHANG Hongwen, MA Wanli, WANG Lei, CHEN Mingchang 2015 Machinery and Electricity Engineering College, Shihezi University, Shihezi, Xinjiang 832000, China

Motion Simulation Analysis Of Yield Monitor Device On Tomato Harvester Based On Solidworks Motion, Yuan Changfu, Zhang Hongwen, Ma Wanli, Wang Lei, Chen Mingchang

Food and Machinery

In order to study the influence of the initial velocity of tomato on weighing accuracy of yield monitor, a motion simulation model of yield monitor on tomato harvester was established. The tomato on the harvester was chosen for the research object. The analysis of motion process was completed by applying a method combined the motion theoretical analysis and motion simulation analysis. The results showed that in order to avoid the accuracy of the motion symmetry of tomato, tomato in the range of the load belt before entering the weighing belt is relatively static, and the initial movement speed of tomato …


Simulation And Optimization Of Spray In Eggs Cleaning System, CHEN Yonggang, YAN Qiusheng 2015 Dongguan Polytechnic, Dongguan, Guangdong 523808, China;Guangdong University of Technology, Guangzhou, Guangdong 510006, China

Simulation And Optimization Of Spray In Eggs Cleaning System, Chen Yonggang, Yan Qiusheng

Food and Machinery

No abstract provided.


Simulation Analysis On A Stuffing Machine Molding Cutter Based On Solidworks, XU Huajun, TANG Ping 2015 Anhui Techincal College on Water Rresoures and Hydroelectric Power, Hefei, Anhui 231603, China

Simulation Analysis On A Stuffing Machine Molding Cutter Based On Solidworks, Xu Huajun, Tang Ping

Food and Machinery

No abstract provided.


Preparation And Experimental Study On Online Removing Inpurities And Sorting Device In Tomato Sauce Factory, LIU Zhiyong, YANG Jiapeng, LIU Junxia, WANG Xiaohui, LIU Wen, YUAN Yong, SUN Tianlong, HA Iling 2015 Xinjiang Institute of Engineering, Urumqi, Xinjiang 830091, China

Preparation And Experimental Study On Online Removing Inpurities And Sorting Device In Tomato Sauce Factory, Liu Zhiyong, Yang Jiapeng, Liu Junxia, Wang Xiaohui, Liu Wen, Yuan Yong, Sun Tianlong, Ha Iling

Food and Machinery

Tomato sauce factory impurities removing mainly rely on a large number of manpower. It is not only time wasting, low collecting effect, and the food hygiene is also difficult to secure. The study developed an automatical removing impurity sorting device which can alternative artificial clean up the weeds and impurity. The device was composed of the bracket, roller, non-slip pattern conveyor belt, side baffle, baffle plate, motor, reducer, photoelectric sensor and automatic control system. The experimental analysis demonstrated that when removing impurity sorting machine working angle between 25° to 45°, the device selected fruit rate and the rate of removing …


An Apple Quality Grading System Based On Motion Controller, WU Xiaoqiang, HUANG Yunzhan, ZHAO Yongjie 2015 Inner Mongolia University for the Nationalities College of Mechanical Engineering College, Tongliao, Inner Mongolia 028000, China

An Apple Quality Grading System Based On Motion Controller, Wu Xiaoqiang, Huang Yunzhan, Zhao Yongjie

Food and Machinery

A kind of apple quality grading system is designed based on motion controller and machine vision system, the system includes the detection module, image acquisition and processing module motion control module and execution module and other components. The operation is simple and accuracy is high, and it can realize the automation of apple quality grading, improve the labor efficiency. The non-contact structure is applied, the structure can overcome the previous mechanical damage caused by the contact of apple. It can ensure the quality after the apple classification. Research shows that this system is high reliability, good openness, grading error less …


Design Of Temperature Remote Monitoring System For Beer Pasteurization Machine Based On Gsm, ZHAO Fang, ZHENG Xiaoxi, QI Zegang 2015 Zhengzhou Technical College, Zhengzhou, Henan 450121, China

Design Of Temperature Remote Monitoring System For Beer Pasteurization Machine Based On Gsm, Zhao Fang, Zheng Xiaoxi, Qi Zegang

Food and Machinery

The key factor which affects the taste and quality of beer is beer pasteurization machine spray water temperature. The system, which is used to beer pasteurization machine temperature’s automatic control and remote monitoring, is deigned based on MSP430 and GSM wireless communications. The temperature detection adopts DS18B20 digital temperature sensor instead of the traditional platinum RTD, and the MSP430 is main controller with low-power and strong anti-interference ability. The control system can adjust each zone temperature of pasteurization machine in real-time, and the real-time temperature of each zone and abnormal temperature alarm information can be send to the monitoring center …


Design Of A Pitting And Slicing Device For Jujube, WEN Huaixing, ZHOU Gaimei, SHI Pengtao 2015 Department of Mechanical and Electrical Engineering, Shaanxi University of Science and Technology, Xian, Shaanxi 710021, China

Design Of A Pitting And Slicing Device For Jujube, Wen Huaixing, Zhou Gaimei, Shi Pengtao

Food and Machinery

A kind of pitting and slicing integrated device was designed. The material feeding was completed by material panel, hairbrush, slider-crank mechanism; The positioning clamp for jujube was accomplished by a flexible device connected with spring; The pitting can achieved by trough type cam mechanism and slider-crank mechanism; Use the Geneva mechanism to finish the intermittent feeding and intermittent rotating of pitting panel and slicing panel; The slicing was fulfilled by the fixed blade and rotated slicing panel. The results show that the device can meet the requirements of deep processing for jujube, its broken rate was lower than 5%.


Development Of Green Walnut Peeling Machine Block, WU Dongze, ZHENG Jiahong, LIANG Jinsheng, NIU Shuoya 2015 College of Mechanical and Electrical Engineering, Shaanxi University of Science & Technology, Xian, Shaanxi 710021, China

Development Of Green Walnut Peeling Machine Block, Wu Dongze, Zheng Jiahong, Liang Jinsheng, Niu Shuoya

Food and Machinery

Combined with the green walnut peel machine exist peeling the peel into a drip shape, design a kind of economical and practical green walnut peel block machine on the study of green walnut peel principle, which has carried on the design of cylinder, peeling mechanism. The structure of the machine is simple, convenient, economic, and safety. The efficiency of green walnut peel is about 98.33%,and the damage rate is 2%. It reduces the machine corrosion by the green peel juice from the peeling process and environmental pollution, which has a very good development prospect.


Design And Research On Compliant Manipulator For Shrimps Head Cutting, HAN Cui, ZHANG Xiuhua, LI Na, WANG Zehe, YI Jinggang 2015 Mechanic and Electronics College, Agriculture University of Hebei, Baoding, Hebei 071001, China

Design And Research On Compliant Manipulator For Shrimps Head Cutting, Han Cui, Zhang Xiuhua, Li Na, Wang Zehe, Yi Jinggang

Food and Machinery

Designed a compliant manipulator device to cut shrimp head, and used the flexible deformation materials to complete the trajectory. It is proved the best by experiments that the knife went along the trajectory from the back force of shrimp to head. Not only can achieve the best results, but also gains access to a higher headless shrimp yield and good sensory quality. Three dimensional modeling was conducted by using Solidworks software, and the motion simulation by ADAMS software, Through the manufacture and experiments of the compliant manipulator, the headless shrimp yield was improved to 70.78%. The device was simple and …


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