Forensic Science and Technology Commons^™

Prevalence Of Non-Self Dna On Three Different Sebaceous Skin Locations, Jan Kirstine Zamora

Student Theses

This project serves to provide additional information on the prevalence of non-self DNA on the exposed skin locations specifically the inner upper arm, the nape area of the neck and the neck area just below the ear, where current literature is lacking. Skin tape lifts from these locations were collected from 15 male participants and 13 female participants once a week over a period of three weeks. Only single source profiles were obtained from six volunteers. Eight volunteers had only one mixture DNA profile in nine samples, while mixture DNA profiles for at least two collection occasions were observed in …

Method Optimization For The Determination Of Cannabinoids In Blood By Liquid Chromatography Tandem Mass Spectrometry (Lc-Ms/Ms), Kevin D. Carrera

Student Theses

Delta-9-tetrahydrocannabinol (THC) continues to be one of the most popular drugs in the USA. Along with THC, other cannabinoids such as cannabidiol (CBD), are on the rise due to an increase in medicinal usage as well as the passage of different legislations removing these products from their current schedule I status. Thus, an urgency exists to develop robust and sensitive analytical methods to determine cannabinoids, especially THC, CBD and metabolites, in biological samples. The purpose of this study was to investigate different analytical procedures to determine the best method to identify and quantify CBD, THC and their metabolites in whole …

Seasonal Effects On The Arrival And Colonization Of Blow Flies (Diptera: Calliphoridae) On Carrion In New Jersey, Amanda A. Hernandez

Student Theses

Blow flies (Order: Diptera, Family: Calliphoridae) are a valuable forensic tool. Female blow flies will lay eggs (colonize) on remains often within minutes of death and entomological methods are based on their behavior and development to extrapolate postmortem intervals (PMI). External factors, such as temperature and weather patterns can influence timing of this behavior. This study seeks to gain a better understanding of the environmental factors that impact this behavior across two seasons. In this study, arrival and colonization behavior was investigated on rat carcasses from sunrise to sunset in a semi-rural region of New Jersey (n=64). Video recording software …

A Comparison Of Methods Used For The Elimination Of Organics From Soil Prior To Analysis By Ftir-Atr And Sem-Eds, Argeliz Pomales

Student Theses

This project suggests improvements to the treatment methods utilized for the sample preparation and evaluation of minerals in soil samples encountered in forensic science casework and research. The evaluation of pre-treated and post-treated soil samples were performed using Fourier-Transform Infrared Spectroscopy with Attenuated Total Reflection (FTIR-ATR) and Scanning Electron Microscopy-Energy Dispersive Spectroscopy (SEM-EDS). Because soil samples contain a significant amount of organic matter and water which interfere with examination of these minerals while using these instruments, pretreatment methods are necessary to isolate the mineral fraction for examination and analysis.

Soil samples were first sieved with various size stainless steel mesh …

Effects Of External Decontamination Of Methamphetamine In Juvenile Hair, Rebecca Mitrani

Student Theses

Methamphetamine is a common illicit substance abused in the United States. Hair is one matrix that is analyzed to determine the presence and concentration of methamphetamine. When hair is analyzed, an external decontamination step is commonly performed prior to drug extraction to avoid a potential positive result from environmental exposure in someone that has not ingested the drug themselves. In child-custody scenarios, however, it is important to know if a child is often around such illicit substances. This study serves to assess if there is a significant difference in methamphetamine concentrations in samples from children up to 12 years old …

Investigating The Mechanisms Driving Mercury Reduction And Emissions In Soil, Hamil Patel

Student Theses

The water-induced emission of mercury from soil has been studied, but a complete understanding of the mechanisms driving mercury reduction and emission in soil has not been reached. The current understanding is an immediate expulsion of soil gaseous mercury upon water treatment and an upward transport of dissolved soil ionic mercury as the water evaporates. We analyzed emissions from several types of samples using various methods to further our understanding on the mechanisms involved. Light-induced emissions from aqueous samples of humic acid (HA) spiked with mercury (II) chloride (HgCl₂) showed that at higher concentrations of HA, less mercury …

Elisa Validation Method For The Detection Of Ketamine In Hair, Daria A. Centonza

Student Theses

An ELISA method was developed and validated to detect ketamine in human hair samples. Ketamine is an anesthetic drug that causes memory loss, dissociative sensations, and hallucinations. Due to these adverse effects, ketamine is a common drug used in drug facilitated sexual assaults (DFSAs). It is very important to be able to detect the substances used in DFSAs over a longer period of time due to the delayed reporting of these crimes. Victims, often out of fear and from the sedative/memory loss effects of the drugs, tend to report these crimes when it is too late to use urine and …

Increasing Gas Chromatography-Flame Ionization Detection Sensitivity For Carfentanyl Detection: An Examination Of Procaine At Different Concentrations And Split Ratios, Sonalia T. Balli

Student Theses

An opioid abuse crisis in recent years has contributed to many overdoses and overdose-related deaths in the United States. In 2017, fentanyl and fentanyl analogs contributed to more than half of NYC’s overdose deaths in 2017. Carfentanyl – an extremely dangerous fentanyl analogue 100 times more potent than fentanyl – is very difficult to detect in drug samples because it is typically present in concentrations lower than analytical instruments can detect at standard settings. In this work, I hypothesize that gas chromatography-flame ionization detection (GC-FID), when optimized for the detection of carfentanyl by adjusting the split ratio, is more sensitive …

Stigma Towards Offenders Diagnosed With Mental Illness, Christina N. Yocca

Student Theses

Mental health stigma is a key factor to what makes members of the community who are diagnosed with mental illness feel like outcasts to the “normal” population. This study investigated the relationship between right-winged beliefs and offenders diagnosed with mental illness. Sigma as it relates to mental health, offenders, and right-winged beliefs have been studied separately before, but there is a lack of information on the stigma that the public has towards offenders who struggle with their mental health and the possible predictors of stigma towards this group. Overall, this study aimed to further research in the intersectionality of stigmatized …

Evaluating A Test For Shedding Propensity Using Tape Lifts From Different Skin Locations, Xiao M. Chen

Student Theses

The shedding propensity of a person can assist data interpretation in casework when assessing the possibility of passive transfer for DNA analysis. Past studies on shedding propensity evaluated palmar skin (washed and unwashed) deposits. This study compared different skin locations with respect to shedding propensity, and explored the potential of tape-lifts as a skin surface collection method. Eight different skin types and samples were collected with adhesive tape disks from 28 participants over three non-consecutive days; the washed and unwashed fingers from both hands, toe, and arm, neck below ear, and nape. Samples were extracted, quantified, amplified, genotyped, and evaluated …

Reproducibility Of Individual Dna Deposits Detected Through Cellular Fluorescence, Natalee Small-Davidson

Student Theses

Contact traces are an important part of DNA casework, but the probative value of any identified associations depends on the possibility of passive transfer. There is known individual variation in DNA left behind during contact, this DNA shedding propensity has an effect on whose DNA is detected. This study evaluated this variability using a cell staining approach. Volunteers were asked to deposit a fingerprint on a clean glass slide, then wash their hands and deposit a second fingerprint after a 30-minute wait without touching anything. Three sets of samples were collected over three consecutive weeks. Fingerprints were stained with a …

Detecting Gsr Indicative Particles On Decayed Bones Using A Novel Field Kit, Sven Engling

Student Theses

Decomposed human remains are complex forensic puzzles, escalating in difficulty as the remains’ age obscures evidence, like trauma. Research has shown that scanning electron microscopes with energy dispersive X-ray spectrometers (SEM-EDX) are capable of detecting and identifying gunshot residue (GSR) particles on bones. However, SEM-EDX work is time consuming, expensive, and not accessible to every forensic department. Therefore, a preliminary field test capable of detecting GSR indicative particles, like lead, could save departments money and assist in trauma identification. This study examines the viability of using either the 3M Lead Check Test swabs or a sodium rhodizonate solution as part …

The Influence Of Demographic Information On Public Attitudes Towards Individuals Who Commit Sexual Offenses, Emily Bogdan

Student Theses

Research exploring the factors that shape public attitudes towards individuals who commit sexual offenses is needed to inform policy and reduce stigma that these individuals face as they reenter society. Prior research has explored demographic factors of those who offend and have been victimized, but few have studied how these variables may interact with one another to shape attitudes toward people who commit sexual offenses. The current study explores whether offender gender, victim gender, and victim age shape the public’s attitudes towards these individuals. Participants were presented with a vignette describing the offense and then they were asked to respond …

Dna Extraction And Genotyping From Burned Skeletal Remains, Kayla M. Rooney

Student Theses

In this research, bovine leg bones were burned, both with and without meat intact, in order to determine if it was possible to extract and amplify DNA to obtain profiles. The meat was burned over an open flame and the DNA was extracted using the Qiagen DNeasy Blood & Tissue Mini Kit, quantified with the NanoDrop, and amplified using the Bovine Genotypes Panel 3.1 Kit, and separated using the 3500 Genetic Analyzer. Profiles were analyzed using the Thermo Fisher Cloud Microsatellite Analysis software. The samples burned with the meat intact produced lower quantities of DNA than the bones burned with …

Effects Of Improper Ammunition Storage, Victoria S. Andre

Student Theses

Firearms are commonly used by law enforcement, hunters, and civilians either for protection, sport or criminal activity. Ammunition is used in traditional firearms such as rifles and handguns, and consists of a cartridge case, propellant, a bullet (projectile), and primer. The two types of priming systems within the cartridge that are commonly used today are rimfire and centerfire cartridges. There are several types of malfunctions that can occur with ammunition. Improper ammunition storage is one major factor that plays a role in ammunition failure. One form of improper ammunition storage is where the storage environment being used abruptly changes due …

Cocaine, Cocaethylene And Ethanol In Postmortem Blood Submitted To The New York City Office Of Chief Medical Examiner, Jada T. Alexis

Student Theses

Cocaine overdose deaths are on the rise in the United States. This increase may be due to the co-consumption of cocaine with other drugs. When cocaine is ingested in conjunction with alcohol, a specific metabolite called cocaethylene forms. Information regarding cocaethylene, specifically its interpretive value in post-mortem cases, has not been widely studied. Data from the Office of the Chief Medical Examiner in New York City revealed that there were 195 cases in 2017 positive for cocaethylene. These cases were most likely to involve black (42.1%) males (79.5%) in Manhattan (28.2%) aged 50 - 59 (34.4%). Most of the cases …

Investigation Of Postmortem Methamphetamine Cases Submitted To The New York City Office Of Chief Medical Examiner, Isaiah Jewell

Student Theses

Methamphetamine (N-methylamphetamine) is a central nervous system stimulant (CNS) and sympathomimetic drug with a high addiction potential. In the United States, there has been a significant increase in the presence of methamphetamine in recent years, specifically in the Northeastern region of the country. The New York City Office of Chief Medical Examiner (NYC-OCME) postmortem methamphetamine casework from 2018 and 2019 was analyzed and revealed that the presence of the drug increased drastically within a span of one year, jumping from 65 cases in 2018 to 99 cases in 2019. Males were overwhelmingly responsible for much of the casework, taking up …

Bullet Ricochet Of Polymer Coated Bullets, Stephen Daly

Student Theses

Polymer coated bullets were designed to limit a shooter’s exposure to lead. However, despite the advantages that these bullets give there are still difficulties which arise in a forensic firearms examination. Unlike in a typical metal coated bullet, individualizing striations will typically not be imparted to a polymer coated bullet making it unable to be linked to a specific firearm. Due to this difficulty any information that can be gained at the scene of the crime could be crucial. Some obtainable information includes the angle of ricochet and the angle of incidence of the discharged bullet as well as traces …

Determination Of Human Shedding Propensity Based On Str Results, Genevieve Trapani

Student Theses

Trace DNA evidence may be discovered at a crime scene after having been deposited by a person of interest via active or passive transfer. Based on previous studies, passive transfer of one’s DNA is influenced by their shedding propensity, or probability of depositing a detectable amount of DNA through touch. Determining the shedding propensity of a person of interest can aid in trace DNA interpretation in forensic casework. This study explored STR profile quality and the presence of a DNA mixture for different skin surface locations, including fingertips before and after handwashing. As expected, unwashed fingers showed a higher prevalence …

Criterion Validity Of The Ohio State University Traumatic Brain Injury Identification Method In A Criminal Justice Sample, Sarah M. Monaghan

Student Theses

The purpose of the current study was to determine the clinical utility of the Ohio State University Traumatic Brain Injury Identification Method (OSU-TBI-ID) in a criminal justice sample, by evaluating the criterion-related validity of this instrument. It was hypothesized that this tool could differentiate between incarcerated individuals with or without a history of Traumatic Brain Injury (TBI) on measures evaluating important TBI-related sequalae. The sample consisted of 95 incarcerated men detained at a private correctional facility in a Mid-Atlantic state. Measures used in this study to evaluate executive functioning difficulties, psychiatric difficulties, substance use problems, institutional misconduct and recidivism were …

Effect Of Age And Sex On The Discrimination Of Monozygotic Twins Using Cg18562578 Dna Methylation In Saliva, Carla Pierre Louis

Student Theses

DNA methylation is a type of epigenetic modification that impacts gene expression without disturbing the genetic nucleotide sequence. DNA methylation has the potential to be included in the daily forensic analysis because among other uses, it has the potential to be applied to discrimination of monozygotic (MZ) twins that currently cannot be differentiated via forensic DNA profiling. In previous research, our group showed that DNA methylation at the cg18562578 site in chromosome 3 could be used to discriminate MZ twins. Because studies have shown that DNA methylation measures can be influenced by demographic factors such as sex and age; here, …

Estimating Muzzle To Target Distance From The Physical Characteristics Of A Bullet Hole In Different Wood Substrates, Alan Lee

Student Theses

Determining the muzzle to target distance of a firearm discharge is an integral part in crime scene reconstruction. Shooter distance is most often estimated using the Modified Griess test, which can be used to visualize gunshot residue (GSR) patterns around bullet holes. However, this test has a 3- to 5-feet range limit as the plume of GSR particles can only travel a certain distance past the muzzle. The purpose in this study was to develop a new method that overcomes this range limitation by analyzing the physical damage characteristics of a bullet hole. Test fires were conducted with a .22 …

The Determination Of G1 And G7 Ballistic Coefficients Of .22lr Caliber Ammunition In Gypsum Board Through Doppler Radar Acquisition, Jose A. Colon Jr.

Student Theses

In this experiment, a method of calculating ballistic coefficients of .22LR (long rifle) caliber ammunition was developed using Doppler radar to track the pre-impact and post-impact velocities of projectiles passing through gypsum board. The method demonstrated has distinct advantages in accuracy and flexibility over conventional chronograph projectile tracking that forensic firearm experts should consider when determining the optimal method to obtain a ballistic coefficient (BC). G1 and G7 ballistic coefficients were determined for three .22LR ammunition brands with respective muzzle velocities of approximately 1050ft/s (320m/s), 1230ft/s (375m/s), and 1640ft/s (500m/s) fired from a Ruger 10/22 semi-automatic carbine using an Infinition …

Effects Of Microwave Radiation On The Digestion Of Proteins Involved In Body Fluid Identification, Aaron J. Fox

Student Theses

Body fluid identification plays an important role in understanding how the events during a crime may have taken place. The presence of a body fluid may help identify an individual who committed a crime, while the type of body fluid present may help investigators determine how a crime occurred. Current body fluid identification techniques are not always conclusive and may only suggest the presence of a body fluid type. A protocol developed by the New York City Office of the Chief Medical Examiner (OCME) established a method that confirms body fluid type through mass spectrometry. To identify marker proteins in …

Performance Of A Dna And Protein Co-Extraction With Trypsin In Comparison To Two Proteinase K Based Dna Extraction Methods, Dinura A. Gunatilake

Student Theses

Effective DNA extraction methods are important for forensic applications. The main goal of this experiment was to determine if a newly developed trypsin based protein/DNA co-extraction method applied to contact traces would yield comparable results to a commercial Proteinase K method (QIAamp DNA Investigator Kit) and a Chelex extraction method with Tween 20 used in forensic laboratories. This was tested on 20 sets of sebaceous fingerprints on glass slides.

The results of this study demonstrate the trypsin co-extraction method yielded the highest amount of DNA. In the first comparison, the mean total DNA yields for the trypsin-co extraction method and …

The Relationship Between Impact Energy And Glass Fracture Via .177 Caliber Steel Bbs, Jocenel Beach

Student Theses

Fractured glass is often found at shooting scenes and can provide useful information for crime scene reconstruction. Mechanical glass fracture from a projectile impact can result in different types of fracture patterns. The degree of fracture depends on several factors including: type of glass, the thickness of the glass, curvature (if any), distance from the muzzle, contact angle, and type of projectile. Correlating impact energy to the degree of glass fracture can provide useful information regarding the muzzle-to-target distance for a particular firearm/ammunition combination. This research focuses on the projectile impact velocity of .177 steel BBs shot from a pneumatic …

Further Evaluation Of A Dry Vacuuming Technique For Recovery Of Dna From Handwritten Documents, Christian Hopkins

Student Theses

A previously developed, non-destructive, homemade vacuum method for collecting biological material from handwritten documents had promising DNA results for white copy paper, preserved indented writing, and latent prints. Prior to casework implementation, additional validation experiments are warranted and here the method was tested for different paper substrates. This work describes testing of notebook paper, bank deposit slips, magazine pages, and manila envelopes. The quantity of recovered DNA varied from donor to donor, but the mean quantities showed a trend that can be explained by the different sizes and surface properties of the tested paper types. The rougher paper type, like …

Developing A Maceration Method For Isolation Of Dna From Cancellous Bones, Bryan Bernal

Student Theses

Prior to isolating DNA, it is necessary to remove any biological materials that may be present on commingled remains. Manual maceration of the outer surface is time-consuming. This study will address this issue by developing a simple method for processing bone samples prior to DNA isolation. A liquid-based technique was applied to macerate bone fragments by incubating bone fragments in an enzyme solution, removing potential contaminants, followed by a bone tissue disruption. Swine (Sus scrofa) bones were used to simulate human bones. Microscopic analysis suggested this method was effective for surface material removal. The methods effect on the …

The Application Of A Simple Tissue-Disruption Method To Dna Extraction For Species Identification, Jordana R. Fox

Student Theses

The TissueLyser II was used to disrupt animal bones and plant tissue without dry ice or liquid nitrogen for species identification. Bone fragments of fresh swine (Sus scrofa) ribs were used. A 521bp fragment of the cyt b locus was analyzed, and the BLASTn results confirmed that the origin of all the samples was swine (E-value = 0.0). Bone fragments of bovine, chicken, duck, fish, sheep, and swine were also used to test the procedure. An approximately 600bp fragment of the COI locus was analyzed, and the BLASTn results confirmed the origin of all the samples (E-value = …

Archaeology Or Crime Scene? Teeth Micro And Macro Structure Analysis As Dating Variable, Jessica A. Vincenty

Student Theses

Simple methods to aid in the determination of forensic or archaeologic relevancy of skeletonized remains have been researched since the 1950s. With advances in microscopic imaging techniques and machine learning computer data analysis methods the relevancy of decontextualized, comingled remains has room for improvement. This thesis is a study done to pioneer a new approach to analyzing dental skeletal remains to determine forensic relevancy.

Archaeological dental samples collected from the ancient city of Ur in modern day southern Iraq in addition to modern dental extractions were processed for scanning electron microscopy imaging. Archaeological and modern samples displayed different surface and …