Forensic Science and Technology Commons^™

Recovery Of Nuclear Dna From Skeletal Remains Decomposed In Freshwater, Zoe L. Sikon

Master's Theses

This research compared freshwater and dry decomposition environments and their impact on DNA recovery from Sus scrofa domesticus(domestic pig) femora over a 3-month period. Prior to decomposition, initial cuttings were taken from "fresh" bone samples. Cuttings were then collected at 3-week intervals from waterlogged and dry bone samples. All samples were extracted using the vendor recommended protocol for the Applied Biosystems PrepFiler BTA extraction kit and an updated version of the same protocol, that substitutes Proteinase K with additional Dithiothreitol (DTT) (Thermo Fisher Scientific, 2012.). DNA quantitation was performed using pig-specific custom primer sets, with the QuantStudio 5 Real-Time …

The Application Of Particle-Correlated Raman Spectroscopic Analysis Of Soils To Mock Casework Scenarios, Sam Gong

Master's Theses

Soil is a continuous material, reaching across geological bodies, and is distinctly variable and differentiable based on aspects such as geographic location, seasonal factors, and human interference. In combination with its highly transferable nature, the complexity of soil makes it valuable material for forensic trace evidence and object-to-scene association. The development of forensic soil analysis is currently focused on mineralogy. Raman spectroscopy is a chemical technique that has previously been studied for mineralogy uses. Additionally, Raman spectroscopy has been researched for use in other fields of forensic science as well. As such, it is possible to use Raman spectroscopy for …

Effects Of Ethanol On Gaba Transaminase, Mary Corrigan

Master's Theses

Ethanol is the primary active ingredient in alcoholic beverages, and functions in the body as a central nervous system (CNS) depressant. The overt effects ethanol has on the body, e.g. diminished cognitive ability, loss of motor control, slurred speech, impaired balance, etc., are well documented. While the behavioral consequences of consumption are well understood, how ethanol actually functions on a mechanistic level to elicit those effects has yet to be satisfactorily elucidated.

The structural homology between ethanol and the C3 and C4 carbons of gamma-hydroxybutyric acid (GABA), the major inhibitory neurotransmitter in the mature CNS, has led us to hypothesize …

The Detection Of Blood And The Recovery Of Dna From Heat-Damaged Surfaces, Teresa A. Distefano

Master's Theses

When investigating a scene exposed to high temperatures and long burn times, investigators should consider that recoverable blood could be linked to a source and give important profile information after DNA analysis. By locating blood with presumptive and confirmatory reagents, even after exposure to damaging conditions, investigators can attempt not only to confirm that blood was present but also to determine the DNA profile of the blood. Experimenting with the different burn times and temperatures in which blood is still detectable can be followed with DNA analysis, which will show whether the blood is viable after exposure to these damaging …

An Evaluation Of The Effect Of Degradation On Irisplex Snps, Maria Gruber

Master's Theses

The information from short tandem repeats (STRs), the current standard forensic DNA typing technology, does not always result in identification. In these cases, there are other informative markers in DNA that can be used to help generate investigative leads, one of which is single nucleotide polymorphisms (SNPs). One of the uses of SNPs is for phenotype determination. Among the phenotypic characteristics that can be predicted is eye color. A commonly used tool for eye color prediction is the free, online statistical prediction model IrisPlex, which includes 6 SNPs known to control eye color.

While SNPs can be useful, they, like …

Trace Dna Detection Using Diamond Dye: A Recovery Technique To Yield More Dna, Leah Davis

Master's Theses

This study aspires to find a new screening approach to trace DNA recovery techniques to yield a higher quantity of trace DNA from larger items of evidence. It takes the path of visualizing trace DNA on items of evidence with potential DNA so analysts can swab a more localized area rather than attempting to recover trace DNA through the general swabbing technique currently used for trace DNA recovery. The first and second parts consisted of observing trace DNA interaction with Diamond Dye on porous and non-porous surfaces.

The third part involved applying the Diamond Dye solution by spraying it onto …

The Impact Of Bone Marrow Transplantation On Forensic Human Identification And Genetic Genealogy Testing, Lisa A. Sikop

Master's Theses

Forensic Investigative Genetic Genealogy has rapidly evolved to become a highly effective investigative tool to assist with identifying perpetrators of violent crimes and unidentified human remains. With forensic DNA (STR) analysis, it has previously been researched that a person who has received a bone marrow transplant (BMT) may produce a multi-allelic STR profile, as both the bone marrow donor and the recipients' DNA is present. A multi-allelic STR profile can easily be misinterpreted as a mixture DNA profile, when in fact it is a chimeric DNA profile originating from a single individual. While the impact of chimerism on STR profiles …

Evaluation Of Raman Microspectroscopy And Hyperspectral Imaging As A Screening Tool To Determine Optimal Sampling Sites For Dna Recovery From Human Skeletal Remains, Kathleen M. Smith

Master's Theses

Numerous challenges exist with forensic genetic testing of human skeletal remains due to diagenesis patterns in bone microstructure, DNA degradation, and the presence of PCR inhibitors. Diagenesis is the microscopic breakdown of the bone matrix, which consists primarily of mineralized calcium hydroxyapatite and collagen. The process of diagenesis occurs in a heterogeneous, non-uniform manner along the diaphysis of a long bone, and determining the region with the most intact bone microstructure is not possible with the naked eye. Therefore, taking cuttings from the diaphysis for DNA testing is somewhat of a “blind” process, and decades of research and casework have …

The Rise Of Terrorism In Africa And What It Means For U.S. Policymakers, Bridget Mary Hughes

Master's Theses

This thesis utilizes three case studies to measure the rise of terrorism in Africa. This qualitative data leads the reader to one ultimate question: what does the rise in terrorism in Africa mean for the United States (U.S.) and U.S. policymakers, National Security academia, students in National Security, if anything at all? The answer is: if civilian and military compounds are being targeted by these violent extremists, as these three case studies demonstrate, it is my recommendation that U.S. policymakers cease sending troops to the African theater and, instead, allocate funding to the citizens and nonprofits of the countries whom …

Quantifying Pain: A Ratio Of Cortisol, Substance P, And Pituitary Adenylate Cyclase-Activating Polypeptide As Biomarkers For Pain In Animal Cruelty And Neglect, Danielle Sherer

Master's Theses

Understanding the relationship between cortisol, Substance P, and PACAP during experienced pain and recovery, as well as the contrast between expression levels and serum levels, will provide an objective measure of pain and suffering to aid in the prosecution of animal cruelty and neglect cases.

There is no consensus at this time as to a recognized biomarker for pain experienced by an animal. Cortisol, Substance P, and Pituitary Adenylate Cyclase-Activating Peptide (PACAP) have been investigated separately in various animal and human studies with regard to stress, pain, and injury. Cortisol is a steroid hormone in the glucocorticoid class that has …

Evaluation Of Raman Spectroscopy As A Screening Tool To Predict Dna Recovery Potential In Skeletonized Human Remains, Cody Silverman

Honors Theses

The purpose of this study is to determine if Raman spectroscopy can be used as a rapid and non-destructive screening tool to predict DNA recovery potential for bone, and to explore the correlation between the compositional microstructure of bone and preservation of DNA. The two primary structural components of bone are calcium hydroxyapatite and collagen. It is proposed that by analyzing bone using Raman spectroscopy, the presence, state, and distribution of hydroxyapatite and collagen can be measured, which may be useful in predicting if the sample is viable for DNA analysis. The ultimate goal of this study is to develop …

An Investigation Of The Effect Of Temperature And Humidity On The Formation Of Antemortem Root Banding, Mae Griffin

Honors Theses

Microscopical hair analysis has become a controversial practice within forensic science primarily due to issues related to interpretation, and specifically overstating the significance of an association. The validity of microscopical hair comparisons has suffered from intense scrutiny by the forensic community, with one criticism being a paucity of peer reviewed research. An often-unrecognized aspect of microscopical hair analysis is its use in providing reconstructive information based on identifiable features. One example of this is postmortem root band or PMRB. PMRB is the formation of a band slightly above the root bulb and below the skin surface on the hair of …

An Interdisciplinary Analysis Of Dehumanization During The Holocaust, Alissa Marie Davies

Honors Theses

The body of literature surrounding the Holocaust and dehumanization is extensive. Multiple theories have been proffered to try and explain the Holocaust’s causation, each with varying degrees of acceptance from the Jewish and global communities. Meanwhile dehumanization has been studied however often through the lens of a singular field. Despite the abundance of research on the Holocaust, the subject of perpetrators has had limited exploration as of yet. The present analysis seeks to examine the mechanics of dehumanization and how these mechanics could explain the behaviors behind the perpetration of the Holocaust and by extension, other genocides. This analysis will …

Forensic Artifact Finder (Forensicaf): An Approach & Tool For Leveraging Crowd-Sourced Curated Forensic Artifacts, Tyler Balon, Krikor Herlopian, Ibrahim Baggili, Cinthya Grajeda-Mendez

Electrical & Computer Engineering and Computer Science Faculty Publications

Current methods for artifact analysis and understanding depend on investigator expertise. Experienced and technically savvy examiners spend a lot of time reverse engineering applications while attempting to find crumbs they leave behind on systems. This takes away valuable time from the investigative process, and slows down forensic examination. Furthermore, when specific artifact knowledge is gained, it stays within the respective forensic units. To combat these challenges, we present ForensicAF, an approach for leveraging curated, crowd-sourced artifacts from the Artifact Genome Project (AGP). The approach has the overarching goal of uncovering forensically relevant artifacts from storage media. We explain our approach …

Forensicast: A Non-Intrusive Approach & Tool For Logical Forensic Acquisition & Analysis Of The Google Chromecast Tv, Alex Sitterer, Nicholas Dubois, Ibrahim Baggili

Electrical & Computer Engineering and Computer Science Faculty Publications

The era of traditional cable Television (TV) is swiftly coming to an end. People today subscribe to a multitude of streaming services. Smart TVs have enabled a new generation of entertainment, not only limited to constant on-demand streaming as they now offer other features such as web browsing, communication, gaming etc. These functions have recently been embedded into a small IoT device that can connect to any TV with High Definition Multimedia Interface (HDMI) input known as Google Chromecast TV. Its wide adoption makes it a treasure trove for potential digital evidence. Our work is the primary source on forensically …

Duck Hunt: Memory Forensics Of Usb Attack Platforms, Tyler Thomas, Mathew Piscitelli, Bhavik Ashok Nahar, Ibrahim Baggili

Electrical & Computer Engineering and Computer Science Faculty Publications

To explore the memory forensic artifacts generated by USB-based attack platforms, we analyzed two of the most popular commercially available devices, Hak5's USB Rubber Ducky and Bash Bunny. We present two open source Volatility plugins, usbhunt and dhcphunt, which extract artifacts generated by these USB attacks from Windows 10 system memory images. Such artifacts include driver-related diagnostic events, unique device identifiers, and DHCP client logs. Our tools are capable of extracting metadata-rich Windows diagnostic events generated by any USB device. The device identifiers presented in this work may also be used to definitively detect device usage. Likewise, the DHCP logs …

Another Brick In The Wall: An Exploratory Analysis Of Digital Forensics Programs In The United States, Syria Mccullough, Stella Abudu, Ebere Onwubuariri, Ibrahim Baggili

Electrical & Computer Engineering and Computer Science Faculty Publications

We present a comprehensive review of digital forensics programs offered by universities across the United States (U.S.). While numerous studies on digital forensics standards and curriculum exist, few, if any, have examined digital forensics courses offered across the nation. Since digital forensics courses vary from university to university, online course catalogs for academic institutions were evaluated to curate a dataset. Universities were selected based on online searches, similar to those that would be made by prospective students. Ninety-seven (n = 97) degree programs in the U.S. were evaluated. Overall, results showed that advanced technical courses are missing from curricula. We …

Amino Acid Composition Analysis As A Means To Differentiate Hair Samples From Individuals Of Similar Demographics And The Effect Of Hair Treatments, Trisha Brady

Honors Theses

Hair is a forensically-relevant exhibit due to its ability to be shed, torn, or transferred during a crime. Current forensic hair analysis includes microscopic hair comparison and mitochondrial or nuclear DNA analyses, each with limitations. Novel methods, such as those evaluated herein, have potential to complement conventional techniques. These include evaluating functional groups with infrared (IR) spectroscopy, elements with laser induced breakdown spectroscopy (LIBS), and amino acid ratios with gas chromatography- mass spectrometry (GC-MS). Hair from two demographically similar individuals was divided into three sample groups per individual: untreated, bleached, and dyed. Spectral differences between individuals and treatments were evaluated …

Evaluation Of The Effect Of The Humic Acid Inhibitor On Forensic Genetic Investigations Of Human Skeletal Remains, Madeline King

Honors Theses

Postmortem survival of DNA in human skeletal remains occurs due to the compact microstructure of the skeleton and its ability to provide a strong, protective physical barrier to environmental insults. On a molecular level, DNA preservation in bones/teeth involves electrostatic interactions between the negatively-charged DNA backbone and positively charged calcium residues in hydroxyapatite, the latter of which is one of the main components of bone microstructure. Despite these protections, over time endogenous DNA becomes damaged, limiting our ability to detect it and affecting its utility in making a positive identification. Hence, forensic genetic investigations of unidentified human remains (UHRs) are …

Extraction Of Human Dna From Soil In A Simulated Clandestine Grave, Arely Joaly Parra Lopez

Honors Theses

Locard’s Exchange Principle states that “every contact leaves a trace.” The same principle applies when a perpetrator of a homicide decides to bury a victim in a clandestine grave. If a perpetrator originally buried a murder victim in haste to prevent discovery and then decided to later move the victim’s body to a remote location for disposal, decomposition fluids from the victim’s body (which contain cellular material and therefore DNA) would remain in the surrounding grave soil at the original burial site. It is possible that investigators could: 1) prove that a human body had once laid in that location …

Investigating The Use Of Saligae® To Identify Expirated Blood On Various Material, Maria Bigos

Honors Theses

Expirated bloodstains and impact bloodstains have very similar physical characteristics. The main way Blood Pattern Analysts identify bloodstains in based on their physical characteristics. This can lead to difficulty distinguishing between expirated blood and impact spatter. Expirated blood can be identified through the presence of air bubbles, however these are often only present on non-porous surfaces. However, if expirated bloodstains can be identified through the presence of saliva, this can provide an alternative way to identify expirated bloodstains. This study examined if SALIgAE can be accurately used to identify the presence of saliva in blood and thus, be a way …

Correlation Between Tooth Root Translucency, Crystallinity, And Dna Preservation In Teeth For Unidentified Human Remains (Uhr) Investigations, Paul Yount

Master's Theses

Endogenous genetic material protected by the rigid, mineralized hard tissues of bones and teeth is often targeted for forensic DNA testing in missing persons and unidentified human remains (UHR) investigations. Although standard approaches have narrowed sample selection to a few optimal skeletal elements (i.e., weight-bearing long bones, teeth), subsampling and decisions beyond this can be challenging due to the non-uniform, heterogeneous pattern of postmortem diagenesis. One particular component of skeletal microstructure — the inorganic mineral matrix [hydroxyapatite (HAp), Ca10(PO4)6(OH)2] — is purported to play a major role in DNA preservation. The potential correlation between molecular changes to skeletal microstructure and …

Investigation Of Palatal Rugoscopy For Forensic Human Identification, Cameron Filipe

Honors Theses

Human identification is critical to the field of forensic science as well as in many sub-disciplines, including forensic pathology, forensic anthropology, and forensic odontology. While few widely-accepted methods of identification dominate the discipline (primarily fingerprint and dental record analysis), the study of palatal rugae patterns (rugoscopy) is an emerging tool in human identification. Some studies suggest that patterns of the palatal rugae are an individualizing characteristic among humans. While the use of rugoscopy for identification has been explored as an identifying marker in the dental and orthodontic communities, its use for forensic applications is not widely recognized. Also, while some …

Zooming Into The Pandemic! A Forensic Analysis Of The Zoom Application, Andrew Mahr, Meghan Cichon, Sophia Mateo, Cinthya Grajeda, Ibrahim Baggili

Electrical & Computer Engineering and Computer Science Faculty Publications

The global pandemic of COVID-19 has turned the spotlight on video conferencing applications like never before. In this critical time, applications such as Zoom have experienced a surge in its user base jump over the 300 million daily mark (ZoomBlog, 2020). The increase in use has led malicious actors to exploit the application, and in many cases perform Zoom Bombings. Therefore forensically examining Zoom is inevitable. Our work details the primary disk, network, and memory forensic analysis of the Zoom video conferencing application. Results demonstrate it is possible to find users' critical information in plain text and/or encrypted/encoded, such …

Memory Foreshadow: Memory Forensics Of Hardware Cryptocurrency Wallets – A Tool And Visualization Framework, Tyler Thomas, Mathew Piscitelli, Ilya Shavrov, Ibrahim Baggili

Electrical & Computer Engineering and Computer Science Faculty Publications

We present Memory FORESHADOW: Memory FOREnSics of HArDware cryptOcurrency Wallets. To the best of our knowledge, this is the primary account of cryptocurrency hardware wallet client memory forensics. Our exploratory analysis revealed forensically relevant data in memory including transaction history, extended public keys, passphrases, and unique device identifiers. Data extracted with FORESHADOW can be used to associate a hardware wallet with a computer and allow an observer to deanonymize all past and future transactions due to hierarchical deterministic wallet address derivation. Additionally, our novel visualization framework enabled us to measure both the persistence and integrity of artifacts produced by the …

Exploring The Learning Efficacy Of Digital Forensics Concepts And Bagging & Tagging Of Digital Devices In Immersive Virtual Reality, Courtney Hassenfeldt, Jillian Jacques, Ibrahim Baggili

Electrical & Computer Engineering and Computer Science Faculty Publications

This work presents the first account of evaluating learning inside a VR experience created to teach Digital Forensics (DF) concepts, and a hands-on laboratory exercise in Bagging & Tagging a crime scene with digital devices. First, we designed and developed an immersive VR experience which included a lecture and a lab. Next, we tested it with (n = 57) participants in a controlled experiment where they were randomly assigned to a VR group or a physical group. Both groups were subjected to the same lecture and lab, but one was in VR and the other was in the real world. …

Investigating The Efficacy Of Dna Damage With Bleach In Forensic Laboratories And At Crime Scenes, Alyssa Tuccinardi

Honors Theses

Household/commercial bleach (6% NaOCl, sodium hypochlorite) degrades DNA through oxidative damage, production of chlorinated base products, and cleavage of DNA strands (breaking it into smaller and smaller fragments). The presence of these lesions significantly impacts the ability to generate a full genetic profile from an evidentiary sample. In fact, knowledge of the damaging effect of bleach on DNA is the basis for its use in forensic laboratories to clean workbenches and prevent cross-contamination of samples between cases. Additionally, bleach is used intentionally by criminals to clean up crime scenes and destroy DNA evidence. A previous study demonstrated that bleach has …

Photofading Of Black Dyes In Cotton And Polyester Fibers Resulting From Environmental Exposure To Sunlight, Maggie E. Mahaney

Honors Theses

Color is one of the most important properties analyzed during forensic fiber examinations due to its discriminatory power. Differing colors in fibers are usually sufficient grounds for exclusion, but in cases where a lengthy period has passed between crime commission and evidence recovery, the high probability of environmental degradation having altered the questioned fibers’ properties could lead to false exclusions. Photofading, the loss of color resulting from exposure to light, is a common form of degradation observed in fibers. Although well-known in the dyeing industry, it is not typically factored into forensic fiber examinations. Published photofading research has been limited …

Small Island Taphonomy In Western Australia, Courtney J. Newberry

Honors Theses

Forensic death investigations rely on postmortem interval estimations to establish a timeline surrounding a decedent’s death. Several methods are used, often together, to make such estimations. One of the main methods is the evaluation of the stage of decomposition; although decomposition follows the same general steps, the length of the process can vary by location and environmental factors. Animal scavenging can also impact decomposition by consuming flesh and impacting insect activity on the remains.

This study investigates the scavenging guilds and rate of decomposition of pig (Sus scrofa) legs on Rottnest Island, Australia. Three sites with unique environments were selected …

Investigating The Potential And Pitfalls Of Ev-Encapsulated Micrornas As Circulating Biomarkers Of Breast Cancer, Brian M. Moloney, Katie E. Gilligan, Doireann P. Joyce, Clodagh P. O'Neill, Killian P. O'Brien, Sonja Khan, Claire L. Glynn, Ronan M. Waldron, Ciarán M. Maguire, Emma Holian, Erin Naughton, Mohamed Elhadi, Andrea B. Grealish, Carmel Malone, Emma Mcdermott, Peter Dockery, Thomas Ritter, Adriele Prina-Mello, Michael J. Kerin, Róisín M. Dwyer

Forensic Science Publications

Extracellular vesicles (EVs) shuttle microRNA (miRNA) throughout the circulation and are believed to represent a fingerprint of the releasing cell. We isolated and characterized serum EVs of breast tumour-bearing animals, breast cancer (BC) patients, and healthy controls. EVs were characterized using transmission electron microscopy (TEM), protein quantification, western blotting, and nanoparticle tracking analysis (NTA). Absolute quantitative (AQ)-PCR was employed to analyse EV-miR-451a expression. Isolated EVs had the appropriate morphology and size. Patient sera contained significantly more EVs than did healthy controls. In tumour-bearing animals, a correlation between serum EV number and tumour burden was observed. There was no significant relationship …