Forensic Science and Technology Commons^™

Potential Applications Of Microrna Profiling To Forensic Investigations, Claire L. Glynn

Forensic Science Publications

Within the forensic science community, there is a continued push to develop novel tools to aid in criminal investigations. microRNA (miRNA) analysis has been the focus of many researcher’s attention in the biomedical field since its discovery in 1993; however, the forensic application of miRNA analysis has only been suggested within the last 10 years and has been gaining considerable traction recently. The primary focus of the forensic application of miRNA analysis has been on body fluid identification to provide confirmatory universal analysis of unknown biological stains obtained from crime scenes or evidence items. There are, however, other forensic applications …

Effect Of 17a - Ethynylestradiol On The Pupation Rate, Emergence Rate, And Sex Ratio Of Phormia Regina (Calliphoridae), Bethany K. Hoschar

Honors Theses

After death, blowflies are attracted to decomposing remains for feeding and reproduction purposes. Since blowflies are usually the first species to colonize a set of remains, they are important to forensic scientists when estimating the time since death. Using the knowledge of the life cycle of a blowfly and any interferences of the environment that can affect the development, forensic entomologists can estimate the time since the arrival of blowflies, otherwise considered time since deposition. This research investigated how a specific estrogen, 17 a-ethynylestradiol, present in pollutants affects the development time of blowflies, and therefore the time since death estimation. …

Founding The Domain Of Ai Forensics, Ibrahim Baggili, Vahid Behzadan

Electrical & Computer Engineering and Computer Science Faculty Publications

With the widespread integration of AI in everyday and critical technologies, it seems inevitable to witness increasing instances of failure in AI systems. In such cases, there arises a need for technical investigations that produce legally acceptable and scientifically indisputable findings and conclusions on the causes of such failures. Inspired by the domain of cyber forensics, this paper introduces the need for the establishment of AI Forensics as a new discipline under AI safety. Furthermore, we propose a taxonomy of the subfields under this discipline, and present a discussion on the foundational challenges that lay ahead of this new research …

Evaluation Of Dna From Blood And Saliva Overtime, Janine Smalling

Honors Theses

In this research, we will examine the quantity of DNA over time. In John M. Butlers, "Fundamentals of Forensic DNA Typing", he charts out the average amount of DNA extracted from different bodily fluid samples. Examples include blood, semen, saliva, urine, etc. Though valuable, the values presented are in the cases of a fresh extraction of the sample. It is important to note that in most cases when DNA is being extracted from crime scene evidence, the samples are not fresh and have been degraded to some extent. On a crime scene, time is only one of the factors affecting …

Haplotype Analysis For Irish Ancestry, Heather Miller Coyle, Robert Whiting

Forensic Science Publications

Forensic haplotype analysis of the male Y chromosome is currently used to establish the number of male donors in sexual assaults, the number of male bleeders in blood pattern analysis, and for ancestry correlation to genetic founder populations in biogeographic studies. In forensic laboratory applications, its primary use is for DNA profile generation with trace amounts of male DNA in the presence of excess female DNA (e.g. spermatozoa identification, male component of fingernail scrapings). Our study supports the potential use of the Y chromosome in a “dragnet” approach (most haplotypes are unique) similar to that described by Kayser in 2017 …

Map My Murder: A Digital Forensic Study Of Mobile Health And Fitness Applications, Courtney Hassenfeldt, Shabana Baig, Ibrahim Baggili, Xiaolu Zhang

Electrical & Computer Engineering and Computer Science Faculty Publications

The ongoing popularity of health and fitness applications catalyzes

the need for exploring forensic artifacts produced by them. Sensitive

Personal Identifiable Information (PII) is requested by the applications

during account creation. Augmenting that with ongoing

user activities, such as the user’s walking paths, could potentially

create exculpatory or inculpatory digital evidence. We conducted

extensive manual analysis and explored forensic artifacts produced

by (n = 13) popular Android mobile health and fitness applications.

We also developed and implemented a tool that aided in the timely

acquisition and identification of artifacts from the examined applications.

Additionally, our work explored the type of …

Iot Ignorance Is Digital Forensics Research Bliss: A Survey To Understand Iot Forensics Definitions, Challenges And Future Research Directions, Tina Wu, Frank Breitinger, Ibrahim Baggili

Electrical & Computer Engineering and Computer Science Faculty Publications

Interactions with IoT devices generates vast amounts of personal data that can be used as a source of evidence in digital investigations. Currently, there are many challenges in IoT forensics such as the difficulty in acquiring and analysing IoT data/devices and the lack IoT forensic tools. Besides technical challenges, there are many concepts in IoT forensics that have yet to be explored such as definitions, experience and capability in the analysis of IoT data/devices and current/future challenges. A deeper understanding of these various concepts will help progress the field. To achieve this goal, we conducted a survey which received 70 …

Multiple Contacts Of Drug Contaminated Fingermarks And Their Analysis With Raman Microspectroscopy, Victoria Deprimo

Master's Theses

This thesis research aimed to determine if substrate, enhancement technique, and multiple contacts affect the detection and identification of drugs in fingermarks using Raman Spectroscopy. It has the potential to be of great importance in forensic science as fingermarks are one of the most important traces left behind at crime scenes and illicit drugs are a significant criminal justice problem. Thus, being able to associate illicit drugs with a specific fingermark has great potential for forensic science, as it can put the drugs in the hands of a specific individual.

The ridges of fingerprints trap trace amounts of material that …

Forensic Characterization And Discrimination Of Manila Envelopes, María Isabel Sánchez Melo

Master's Theses

Envelopes can be found at crime scenes when ransom, threat letters or potentially harmful substances are sent to victims. Therefore, they are important probative items of evidence analyzed by forensic document examiners. Manila envelopes are commonly used in the United States to transport or send documents as they are made of thick and durable paper. Although there are many studies on the forensic analysis of office paper and paper-based banknotes, and only a few on white envelopes, there does not exist previously published research on the physical or chemical characterization of manila envelopes. The goal of this research was to …

Determining The Interference Of Lactate And Lactate Dehydrogenase In An Ethanol Enzyme Assay, Julia Liebl

Master's Theses

Enzymatic ethanol assays are widely used to determine blood alcohol content for individuals admitted to a hospital. In some instances, this hospital data is later used as evidence in a court of law, most commonly in DUI cases. As the enzymatic assay targets, but does not exclusively measure ethanol, it is theoretically possible that interference could occur and produce a falsely elevated result, leading to wrongful convictions or other consequences. This project examined the potential of clinically relevant levels of one potential interferant, lactate and the hepatic enzyme lactate dehydrogenase (LDH) to cause significant and falsely elevated results. Threshold levels …

Investigating The Masking Effects Of Blood On Organic Gunshot Residue Using Gc/Ms, Raman, And Atr-Ftir, Tyler Willits

Master's Theses

Gunshot residue (GSR) can be an indicator for determining the distance of a firearm discharge and linking potential suspects and witnesses to a shooting event. However, the evidentiary value of inorganic gunshot residue (IGSR), commonly used to detect GSR, has been called into question due to the potential for false negatives and false positives. Organic gunshot residue (OGSR) acts as supporting evidence; this increases the overall confidence of the examiner, as well as the value of gunshot residue evidence as a whole.

Current research has focused on three main areas for OGSR. The first is what compounds we should identify …

Dna Methylation Methods For Donor Age Prediction Using Touch Dna, Emily Neverett

Master's Theses

The International Labor Organization (ILO) estimated over 30 million individuals fall victim to human trafficking each year, of which, 50% are children below the age of 16. In 2012, the ILO reported there to be 168 million child laborers worldwide, with many trafficked into hazardous conditions to manufacture consumer products that are sold in developed countries. This is a modern form of slavery with poor working conditions, no access to education, and low wages. The hidden nature of this crime, however, makes it extremely difficult to identify and locate victims of forced child labor, and thus making it challenging to …

Detection Of Larval Aggregations Using A Drone Mounted Thermal Imaging Camera, Megan Descalzi

Master's Theses

When a body’s temperature reaches ambient temperature after death, remote detection of the body can become difficult. At this point, useful search tactics include the use of cadaver dogs, search parties and aerial imaging devices, all of which can be costly and time-consuming for every day law enforcement use. This study investigated the potential of a novel search technique in which a small, unmanned aerial system (drone) mounted with a forward-looking infrared radar (FLIR) was utilized to detect decomposing animal carcasses via the heat generated by associated Diptera larval aggregations. Hot water baths were utilized as analogs for larval aggregations …

Small Rna Sequencing And Rt-Qpcr Validation Of Forensically Relevant Body Fluids, Karly Johannsen

Master's Theses

Body fluid identification is an important aspect of forensic investigations because it assists with the reconstruction of crime scenes and can refute and/or support witness statements. Currently, there is no universal method for body fluid identification. Each body fluid has several tests for its identification, both presumptive and confirmatory. A universal method for body fluid identification that is sensitive, specific, efficient, and minimally destructive is necessary.

In recent years miRNAs have been heralded as novel biomarkers for the identification of body fluids. Several research groups across the US and internationally have been formed to determine which miRNAs are suitable for …

On Efficiency Of Artifact Lookup Strategies In Digital Forensics, Lorenz Liebler, Patrick Schmitt, Harald Baier, Frank Breitinger

Electrical & Computer Engineering and Computer Science Faculty Publications

In recent years different strategies have been proposed to handle the problem of ever-growing digital forensic databases. One concept to deal with this data overload is data reduction, which essentially means to separate the wheat from the chaff, e.g., to filter in forensically relevant data. A prominent technique in the context of data reduction are hash-based solutions. Data reduction is achieved because hash values (of possibly large data input) are much smaller than the original input. Today's approaches of storing hash-based data fragments reach from large scale multithreaded databases to simple Bloom filter representations. One main focus was put on …

Immersive Virtual Reality Attacks And The Human Joystick, Peter Casey, Ibrahim Baggili, Ananya Yarramreddy

Electrical & Computer Engineering and Computer Science Faculty Publications

This is one of the first accounts for the security analysis of consumer immersive Virtual Reality (VR) systems. This work breaks new ground, coins new terms, and constructs proof of concept implementations of attacks related to immersive VR. Our work used the two most widely adopted immersive VR systems, the HTC Vive, and the Oculus Rift. More specifically, we were able to create attacks that can potentially disorient users, turn their Head Mounted Display (HMD) camera on without their knowledge, overlay images in their field of vision, and modify VR environmental factors that force them into hitting physical objects and …

Frameup: An Incriminatory Attack On Storj: A Peer To Peer Blockchain Enabled Distributed Storage System, Xiaolu Zhang, Justin Grannis, Ibrahim Baggili, Nicole Lang Beebe

Electrical & Computer Engineering and Computer Science Faculty Publications

In this work we present a primary account of frameup, an incriminatory attack made possible because of existing implementations in distributed peer to peer storage. The frameup attack shows that an adversary has the ability to store unencrypted data on the hard drives of people renting out their hard drive space. This is important to forensic examiners as it opens the door for possibly framing an innocent victim. Our work employs Storj as an example technology, due to its popularity and market size. Storj is a blockchain enabled system that allows people to rent out their hard drive space …

Construction Of A Reference Allelic Ladder For An Odocoileus Str Multiplex, Jolene Strand

Master's Theses

Odocoileus is a genus of Cervidae (deer) consisting of white-tailed deer (Odocoileus virginianus, Zimmerman, 1780), mule deer (Odocoileus hemionus, Rafinesque, 1817) and blacktailed deer (Odocoileus hemionus hemionus, Rafinesque, 1817). Hunting Odocoileus is only legal with proper permits according to laws that dictate when, where, how, and what can be hunted. Anything outside the legal limits is considered to be poaching. A useful tool for investigating poaching cases is Short Tandem Repeat (STR) DNA analysis. This is commonly used in human forensic casework to link DNA evidence found at crime scenes to either victims or suspects. In poaching cases, it can …

The Potential Interference Of Body Products And Substrates To The Identification Of Ignitable Liquid Residues On Worn Clothing, Gina Guerrera, Erika Chen, Robert Powers, Brooke Weinger Kammrath

Forensic Science Publications

The question of whether deposits on clothing as well as their chemical composition are being mistaken for ignitable fluids is a concern for forensic analysts. Body products and oil secretions can have similar chemical profiles to ignitable liquid residues (ILRs) as a result of comparable chemical compounds that may be found in both sources. This study investigated whether substrates of unworn and worn clothing, with endogenous body secretions and body products could interfere with ILR analysis. Sample extraction was completed by passive headspace concentration with activated charcoal strips (ACS) and desorption with carbon disulfide followed by analysis with gas chromatography-mass …

A Practitioner Survey Exploring The Value Of Forensic Tools, Ai, Filtering, & Safer Presentation For Investigating Child Sexual Abuse Material, Laura Sanchez, Cinthya Grajeda, Ibrahim Baggili, Cory Hall

Electrical & Computer Engineering and Computer Science Faculty Publications

For those investigating cases of Child Sexual Abuse Material (CSAM), there is the potential harm of experiencing trauma after illicit content exposure over a period of time. Research has shown that those working on such cases can experience psychological distress. As a result, there has been a greater effort to create and implement technologies that reduce exposure to CSAM. However, not much work has explored gathering insight regarding the functionality, effectiveness, accuracy, and importance of digital forensic tools and data science technologies from practitioners who use them. This study focused specifically on examining the value practitioners give to the tools …

The Impact Of Environmental Degradation On The Analysis Of Manufactured Fibers, Alexis R. Weber

Master's Theses

The analysis of fiber evidence involves the ability to link a questioned fiber back to its known source. But before the collection and analysis of questioned fibers, they could be potentially exposed to various environmental conditions for an extended period of time. There has been little research on how manufactured fibers are affected by various environments and if this interaction affects the ability to link a questioned fiber back to its known source. Changes to the manufactured fibers are essential to be aware of, in order to avoid the possibility of erroneous exclusions when performing fiber comparisons. A six-month time …

Assay Development For The Identification Of Species And Bioactive Compounds Of Morinda Citrifolia (Noni) In Dietary Supplements By Rt-Qpcr, Dna Sanger Sequencing And Spectroscopy (Uv-Vis/Ftir), Ricardo L. Fernandez Walker

Master's Theses

Regulation standards for dietary supplements lack greatly. Due to this, fraud during the manufacturing of these products might be committed. Previous investigations on herbal dietary supplements have shown that the main ingredient was not present in most of the herbal supplements tested and that these products also contained other substances not stated in the label The purpose of this study was to develop a fluorescent qPCR assay for the identification of Morinda citrifolia herbal species in dietary supplements. Primers for genetic regions rbcL and matK of M. citrifolia were designed. The potential of novel matK primer showed discrimination potential …

Inception: Virtual Space In Memory Space In Real Space, Peter Casey, Rebecca Lindsay-Decusati, Ibrahim Baggili, Frank Breitinger

Electrical & Computer Engineering and Computer Science Faculty Publications

Virtual Reality (VR) has become a reality. With the technology's increased use cases, comes its misuse. Malware affecting the Virtual Environment (VE) may prevent an investigator from ascertaining virtual information from a physical scene, or from traditional “dead” analysis. Following the trend of antiforensics, evidence of an attack may only be found in memory, along with many other volatile data points. Our work provides the primary account for the memory forensics of Immersive VR systems, and in specific the HTC Vive. Our approach is capable of reconstituting artifacts from memory that are relevant to the VE, and is also capable …

Digital Forensics In The Next Five Years, Laoise Luciano, Ibrahim Baggili, Mateusz Topor, Peter Casey, Frank Breitinger

Electrical & Computer Engineering and Computer Science Faculty Publications

Cyber forensics has encountered major obstacles over the last decade and is at a crossroads. This paper presents data that was obtained during the National Workshop on Redefining Cyber Forensics (NWRCF) on May 23-24, 2017 supported by the National Science Foundation and organized by the University of New Haven. Qualitative and quantitative data were analyzed from twenty-four cyber forensics expert panel members. This work identified important themes that need to be addressed by the community, focusing on (1) where the domain currently is; (2) where it needs to go and; (3) steps needed to improve it. Furthermore, based on the …

I Know What You Did Last Summer: Your Smart Home Internet Of Things And Your Iphone Forensically Ratting You Out, Gokila Dorai, Shiva Houshmand, Ibrahim Baggili

Electrical & Computer Engineering and Computer Science Faculty Publications

The adoption of smart home Internet of Things (IoT) devices continues to grow. What if your devices can snitch on you and let us know where you are at any given point in time? In this work we examined the forensic artifacts produced by Nest devices, and in specific, we examined the logical backup structure of an iPhone used to control a Nest thermostat, Nest Indoor Camera and a Nest Outdoor Camera. We also integrated the Google Home Mini as another method of controlling the studied Smart Home devices. Our work is the primary account for the examination of Nest …

Forensic Analysis Of Immersive Virtual Reality Social Applications: A Primary Account, Ananya Yarramreddy, Peter Gromkowski, Ibrahim Baggili

Electrical & Computer Engineering and Computer Science Faculty Publications

Our work presents the primary account for exploring the forensics of immersive Virtual Reality (VR) systems and their social applications. The Social VR applications studied in this work include Bigscreen, Altspace VR, Rec Room and Facebook Spaces. We explored the two most widely adopted consumer VR systems: the HTC Vive and the Oculus Rift. Our tests examined the efficacy of reconstructing evidence from network traffic as well as the systems themselves. The results showed that a significant amount of forensically relevant data such as user names, user profile pictures, events, and system details may be recovered. We anticipate that this …

Survey Results On Adults And Cybersecurity Education, Frank Breitinger, Joseph Ricci, Ibrahim Baggili

Electrical & Computer Engineering and Computer Science Faculty Publications

Cyberattacks and identity theft are common problems nowadays where researchers often say that humans are the weakest link in the security chain. Therefore, this survey focused on analyzing the interest for adults for ‘cyber threat education seminars’, e.g., how to project themselves and their loved ones. Specifically, we asked questions to understand a possible audience, willingness for paying / time commitment, or fields of interest as well as background and previous training experience. The survey was conducted in late 2016 and taken by 233 participants. The results show that many are worried about cyber threats and about their children exploring …

Experience Constructing The Artifact Genome Project (Agp): Managing The Domain's Knowledge One Artifact At A Time, Cinthya Grajeda, Laura Sanchez, Ibrahim Baggili, Devon R. Clark, Frank Breitinger

Electrical & Computer Engineering and Computer Science Faculty Publications

While various tools have been created to assist the digital forensics community with acquiring, processing, and organizing evidence and indicating the existence of artifacts, very few attempts have been made to establish a centralized system for archiving artifacts. The Artifact Genome Project (AGP) has aimed to create the largest vetted and freely available digital forensics repository for Curated Forensic Artifacts (CuFAs). This paper details the experience of building, implementing, and maintaining such a system by sharing design decisions, lessons learned, and future work. We also discuss the impact of AGP in both the professional and academic realms of digital forensics. …

An Overview Of The Usage Of Default Passwords, Brandon Knierem, Xiaolu Zhang, Philip Levine, Frank Breitinger, Ibrahim Baggili

Electrical & Computer Engineering and Computer Science Faculty Publications

The recent Mirai botnet attack demonstrated the danger of using default passwords and showed it is still a major problem. In this study we investigated several common applications and their password policies. Specifically, we analyzed if these applications: (1) have default passwords or (2) allow the user to set a weak password (i.e., they do not properly enforce a password policy). Our study shows that default passwords are still a significant problem: 61% of applications inspected initially used a default or blank password. When changing the password, 58% allowed a blank password, 35% allowed a weak password of 1 character.

Investigating The Isolation And Amplification Of Micrornas For Forensic Body Fluid Identification, Kelsie R. O'Leary, Claire L. Glynn

Forensic Science Publications

Background: The discovery of forensic DNA typing evolved molecular biology far beyond what could have been expected in terms of its forensic application, and now there exists other developments in molecular biology which are ready for application to forensic challenges. One such challenge is the identification of the body fluid source of stains recovered from evidence items and crime scenes. Currently there are significant efforts in the research field to develop novel methods for the molecular identification of body fluids, with microRNAs (miRNAs) revealing great potential. MiRNAs have been shown to have high tissue specificity and are less susceptible to …