Biological and Physical Anthropology Commons^™

The Forensic Implications Of Hydrochloric Acid (Hcl) Exposure On Dna Preservation In Human Teeth, Shaelyn Lee Zimmerman

Graduate Student Theses, Dissertations, & Professional Papers

Identity is a human right, and forensic anthropological methods are often aimed at returning identity to decedents. Three main pathways are used for the identification of human remains: fingerprints, dental records, and DNA analysis. Identification efforts may be hindered when perpetrators attempt to obscure the victim’s identity. In cases where corrosive substances, such as hydrochloric acid (HCl) are used, dental comparison and fingerprinting often fail and genetic analysis becomes the best chance of achieving personal identification.

Previous studies have shown teeth are an excellent source of DNA. Large, multi-rooted teeth, such as molars, are often preferred because they contain the …

Characterizing Regional Human Necromicrobiome Communities In A Semi-Arid Desert To Inform Postmortem Interval (Pmi) Estimation, Georgia Rane Swelstad

Graduate Student Theses, Dissertations, & Professional Papers

Accurate estimation of the postmortem interval (PMI) remains challenging in forensic anthropology, particularly in arid and high-elevation environments where decomposition processes and insect activity are highly variable. Postmortem microbial succession has emerged as a promising alternative framework for PMI estimation. However, the effects of climate and elevation on microbial decomposition dynamics remain insufficiently characterized.

This study examines skin-associated microbial succession in human remains decomposing at the Forensic Investigation Research Station (FIRS) in western Colorado, a semi-arid, high-elevation environment. Microbial samples collected from five anatomical sites during the early postmortem interval are analyzed using 16S ribosomal RNA (rRNA) gene sequencing, and …

On Creating Bog Bodies: The Effects Of Ph Value On Dna Decomposition In A Bog Environment, Gabrielle B. Giangreco

Graduate Student Theses, Dissertations, & Professional Papers

Bog bodies have been a consistent area of interest in both archaeology and biological anthropology, due to their high rates of physical preservation. While there are many physical analyses that have been done on these remains, DNA analysis is lacking. Most studies focus on changes to the remains themselves or attempt to determine why they were placed in a bog. This study aims to examine the effects of pH level on the breakdown of DNA on remains placed inside a bog along with any larger differences in the preservation of the remains. The results of this study seem to point …

Establishing Metric Method’S To Identify Variation In Human Biological Sex Using The Upper And Lower Limb Bones Of Non-Population Specific Samples., Roland P. Sanchez

Graduate Student Theses, Dissertations, & Professional Papers

Sex estimation of human skeletal remains is a facet of the biological profile. The pelvis has been suggested as the best source to accurately predicting the biological sex, but not always available after the recovery of human remains. At times, only long bones of human skeletal remains are recovered for identification. It has been shown that metric measurements using long can accurately predict biological sex. The most common methods use discriminant functions to predict the likelihood an individual falls within a certain population. These studies rely on population specific collections of skeletal remains for increased accuracy. It’s advised to avoid …

Hormonal Decay After Death: Evaluating Dhea-S As A Potential Molecular Marker For Postmortem Estimation, Marisa Lydia Novak

Graduate Student Theses, Dissertations, & Professional Papers

Post-mortem interval (PMI) estimation is a central component of forensic anthropological investigation, but traditional methods for estimating time since death lose accuracy after ~72 hours. Recent research indicates that some steroid hormones may undergo predictable degradation patterns after death, offering potential use as biochemical clocks for PMI estimation beyond this threshold. Dehydroepiandrosterone (DHEA), and its sulfate form (DHEA-S) are of particular interest due to their metabolic stability, systemic influence, and association with trauma. This study investigates whether DHEA-S hormonal degradation patterns correlate with time elapsed since death and body temperature, evaluating their potential as biochemical PMI markers. 160 mL of …

Evaluating The Accuracy Of Stature Estimation From Tibial Radiographs Using Known And Newly Developed Regression Formulas, Atlantis Kae Baron

Graduate Student Theses, Dissertations, & Professional Papers

This study assesses the accuracy of stature estimation from tibial radiographs using digital measurements as an alternative to traditional osteometric tools. Stature estimation is crucial in forensic anthropology for identifying individuals, especially cases involving skeletal remains. Traditional methods rely on physical measurements and established regression formulas, such as those by Trotter & Gleser (1958).

This research analyzed tibial radiographs from the Forensic Anthropology Skeletal Trauma (FAST) database with ImageJ software to measure tibial lengths digitally. The sample included 59 tibiae, with measurements compared against three regression formulas; Trotter & Gleser (1958), Simon et al. (2023), and a new regression formula …

Comparative Bioinformatic Methodologies In Paleogenetic Pathogen Identification In The Casas Grandes, Victoria Lynne Giovannangelo

Graduate Student Theses, Dissertations, & Professional Papers

The application of metagenomic tools to further elucidate ancient DNA (aDNA) holds promise in paleopathology, particularly for inferring disease patterns in ancient populations, especially in those that are understudied. This study conducts a comparative analysis of the efficacy of taxonomic pipelines--Kraken 2, HOPS/MALT, and HAYSTAC--in detecting pathogens within highly degraded aDNA samples extracted from 22 Medio Period Paquimé individuals from the Casas Grandes archaeological site in Northwest Mexico. The taxonomic results from the pipelines are compared with contamination detection program mapDamage, although concerns arose about the latter program’s ability to work with prokaryotes. Additionally, the findings are compared against prior …

Umfc #132: A Comprehensive Forensic Case Report, Allison Paige Mitchell

Graduate Student Theses, Dissertations, & Professional Papers

This case report of an individual designated as University of Montana Forensic Case 132 (UMFC 132) serves to partially fulfill the requirements for the Master of Arts (MA) degree in Forensic and Biological Anthropology. UMFC 132 is a complete human skeleton, used as part of the teaching collection in the University of Montana Forensic Anthropology Laboratory. The minimum number of individuals (MNI) of UMFC 132 is 1. The analysis of UMFC 132 was conducted according to bioarchaeological and forensic anthropological standards of skeletal assessment. UMFC 132 was estimated to be a mid-adult, between the ages of [30-40 years] of age …

Peoples Of India: The Use Of Craniometric Data To Distinguish A Separate Ancestral Group, Stephanie A. Craig

Graduate Student Theses, Dissertations, & Professional Papers

The Peoples of India come from an area with a long history of migrations into and out of the area. This area is considered the second most diverse genetically outside of Africa. Many would group these people as Asian due to the country’s location. However, when studies are done on the skeletal remains from this area, the remains tend to give results of mixed or indeterminate ancestry unless the analyst is familiar with identifying Peoples of India. This study aims to determine the Peoples of India as a separate ancestral group separated from Asians, Europeans, and Africans. To do this, …

Comprehensive Case Report Umfc#140, Marymargaret C. Pratt

Graduate Student Theses, Dissertations, & Professional Papers

University of Montana Forensic Collections (UMFC) Case #140 was observed and recorded fall 2024. The information included are a complete biological profile, any taphonomic features or pathological conditions present, and a literature review on forensic odontology due to the severe nature of the teeth. This individual is consistent with a 5'2"-5'6" adult male ranging between the ages of 35-50 of ambiguous population affinity. Evidence of osteoarthritis and bone spurs are present along the vertebral spine, clavicle, and articulation points throughout the post-cranial skeleton. There is severe dental attrition found along the buccal view of the maxillary and mandibular molars. The …

Reimagining The Accuracy Of The Lovejoy Et Al. (1985) Age Estimation Method Of The Auricular Surface Of The Ilium Through Modern Color Photographs, Alyssa L. Edwards

Graduate Student Theses, Dissertations, & Professional Papers

The ability to accurately estimate the age at time-of-death of an individual is crucial in numerous fields, particularly in a biological or forensic context. There are various methods, features, and bones that are utilized for determining the estimated age of an individual. This study dives into the Lovejoy et al. (1985) age estimation method of the auricular surface of the ilium by using the current age phase descriptions against different reference photos. The study tests whether updated color photos, when working collaboratively with the current age phase descriptions, increases the age estimation accuracy when estimating age based on the auricular …

Bearing Witness: Ancient Dna Analysis And The Dynamics Of Food Procurement In A Historic Missoula Chinese Community, Sarah Elizabeth Grenfell

Graduate Student Theses, Dissertations, & Professional Papers

In 2019, construction of a brewery called Cranky Sam Public House in downtown Missoula, Montana provided an unexpected opportunity to recover and preserve late nineteenth and early twentieth century archaeological evidence of a Missoula neighborhood that included a Chinese temple, a Chinese store, and dwellings with Chinese residents. The area, like many urban landscapes in the American West, also included a restricted, or red-light district. Teams of volunteer University of Montana graduate and undergraduate students conducted on-the-spot salvage archaeology monitoring and were able to recover a sample of artifacts and ecofacts from this once-bustling part of Missoula. The objectives of …

Repatriation And A Biological Profile Of Indigenous Remains Of West Texas, Haley P. Rebardi

Graduate Student Theses, Dissertations, & Professional Papers

The Native American Graves Protection and Repatriation Act (NAGPRA) was created in response to the injustice, mishandling, looting, and trafficking of Native American remains and cultural artifacts. However, even with the latest amendment in December of 2023, there are still gray areas in the context and language. Notably, an issue with NAGPRA is the act only includes institutions and organizations that receive and are supported by Federal funding. Consequently, the act does not cover private collections. The main concern in these contexts is the lack of communication, awareness, and/or permission with and from descendant tribes or potential descendants when it …

Complexities Of Companionship: Exploring The Human-Dog Relationship Through Mtdna Analysis Of Bridge River Canine Remains, Seth Warnick

Graduate Student Theses, Dissertations, & Professional Papers

Dogs (Canis lupus familiaris) hold an unquantifiable level of cultural influence and companionship across swathes of societies all over the globe. While dogs are genetically very distinct from Homo sapiens, throughout human history the two organisms have spread across the world together and have biologically gone through similar selective, social, and environmental constraints. Today the biological makeup of dog breeds is incredibly diverse, however, the archaeological record and genetic research has provided insight into the humble beginnings of a relationship between humans and dogs that has transcended the fabric of emerging civilization itself. Located on the Northwest interior plateau, Bridge …

Meta-Analysis Of Scent Detection Canines And Potential Factors Influencing Their Success Rates, Molly Marie Jaskinia

Graduate Student Theses, Dissertations, & Professional Papers

Objective: This is a meta-analysis focused on the success rates of scent detection canines and potential factors that could influence their accuracy. A series of statistical analyses were conducted to determine if certain demographic factors, such as the dog’s gender, age, and breed, have an effect on a scent dog’s accuracy during a search. Or if more circumstantial factors, like the dog’s level of experience in scent work, the type of target scent, and their handler’s awareness of the target’s location, affect the outcome of the search.

Materials and Methods: A dataset was created from 37 different articles consisting of …

Umfc #12: A Comprehensive Forensic Analysis Case Report, Kaylee M. Hinds

Graduate Student Theses, Dissertations, & Professional Papers

The University of Montana Forensic Case 12 (UMFC) is a human skeleton with an MNI of one that was recovered in August 1983 from an old schoolyard in Helena, Montana, by construction workers who were building a highway. The individual was given to the University of Montana Anthropology Department following the authority’s investigation. At the time, the University of Montana aided in the analysis of the skeletal remains and both departments had deemed the case as forensically insignificant and historic. The initial recoverers believed that the individual was a descendant of the Last Chance Gulch era and reflected Chinese ancestry, …

Activity Pattern Analysis From A Commingled And Fragmentary Necropolis: Entheseal Changes At Kourion Amathus Gate Cemetery (Kagc), Hannah Burgess Carson

Graduate Student Theses, Dissertations, & Professional Papers

Entheseal changes (EC) have been used to reconstruct past human activity patterns, but little research has been done in Cyprus or outside of complete, well-preserved remains. The Kourion Amathus Gate Cemetery (KAGC) is a necropolis located on the southern coast of Cyprus. The Late-Roman (1-7 c. CE) cist graves contained commingled and fragmentary remains. Due to the commingled and fragmentary nature of KAGC, the primary purpose of this study was to see if EC analyses could be conducted on this population. Following that, this study sought to observe possible patterns of preservation as well as trends in EC data between …

Ancient Migrations In West Mexico: Mtdna Analyses, Patricio Gutiérrez Ruano

Graduate Student Theses, Dissertations, & Professional Papers

Despite the mounting evidence that suggests The Aztatlán tradition in West Mexico was a major cosmopolitan region during the Postclassic period (AD 900-1521) with connections to the rest of what is now Mexico, archaeologists have characterized items in West Mexico as culturally distinct from the rest of Mesoamerica. Recently, endogenous, and exogenous material culture has been interpreted as movement and exchange of goods and ideas between subregions and surrounding areas, all of which mention physical contact and trade were involved between Aztatlán and elsewhere. This has included interacting with areas as far as the U.S. Southwest, as well as in …

Preliminary Study In More Effective Utilization Of 87sr/86sr Analysis In The Field Of Forensic Anthropology, Samantha Powers

Graduate Student Theses, Dissertations, & Professional Papers

Isotopic analyses are gaining attention in the field of anthropology. The current utilization of strontium isotopes primarily focuses on residence and migration patterns of past populations extracting data from tooth enamel to indicate early-life residence This study aims to identify a skeletal feature to be set as a field standard in strontium analysis of modern remains when seeking end-of-life residence, the impact of diagenesis on modern remains, and to test the accuracy of the petrous as an alternative to tooth enamel.

The results of this research were unable to suggest such a standard but gathered helpful data to be used …

Morphometric Ancestral Analysis Of Infraorbital Foramen And Maxillo-Facial Landmarks Of Adult North American Skulls Using X-Ray And Computed Tomography Scans, Anna-Marie Lynn David

Graduate Student Theses, Dissertations, & Professional Papers

Skeletal remains provide a variety of information about a species population and the sub populations within that species. The infraorbital foramen has previously been used to understand dietary niches, paleoecology, the nervous system, and the effect of the nervous system on other bone functions in the facial region in humans and other mammals. In medicine, the precise location of the infraorbital foramen has been studied to aid and guide maxillo-facial procedures and surgeries. In this research project, the null hypothesis states that the placement and location of the infraorbital foramen in relation to other facial landmarks were the same between …

Identifying Skeletal Trauma Markers Associated With Intimate Partner Violence (Ipv), Haley K. Omeasoo

Graduate Student Theses, Dissertations, & Professional Papers

Objectives: This is a validation study of Biddle (2019), where much of the same materials are used, with the addition of new data from other databases to include more Native American and minority samples. A comparison database will also be analyzed against this data, with known cases of live individuals assaulted and assessed by health officials, some in IPV situations. All data is analyzed to discover which fracture locations are most common in IPV situations and which ethnic, sex, and age groups are most affected. Most fractures analyzed are those of the maxillofacial area, concentrating on the Zygomaticomaxillary Complex (ZMC) …

The Effects Humidity & Temperature Has On Dna Contamination During Storage, Samantha L. Ramey

Graduate Student Theses, Dissertations, & Professional Papers

Studies have been conducted on DNA cross-contamination throughout the criminal investigation process in order to evaluation the possibility that DNA from one sample could potentially influence the outcome of another. However, no published studies have examined the potential for contamination during the storage of genetic evidence after samples have been taken from crime scenes or suspects. This study is a continuation of a preliminary project, which examined storage drying time in relation to cross-contamination. The current study tested temperature and humidity for the potential of cross-contamination during storage prior to extraction. Prior to storage, 50 μl of male saliva was …

A Comprehensive Forensic Case Report With The University Of Montana Forensic Anthropology Lab University Of Montana Forensic Case #167, Tyler J. Trettin

Graduate Student Theses, Dissertations, & Professional Papers

This report consists of an inventory of the skeletal remains of case# 167, assessment of the minimum number of individuals (MNI), a biological profile if possible, and a literature review of the assessment and understanding of ballistic trauma. The skeletal remains are consistent with an MNI of one. The remains are likely from a male individual, and skeletal features are representative of a person of Caucasian ancestry. The individual is estimated to be between of 35-65 years old with an estimated stature of between 5’5” and 5’9”.

Y-Chromosome Dna Extraction From Post-Cranial Skeletal Elements, Mykala D. Ward

Graduate Student Theses, Dissertations, & Professional Papers

The use of DNA in forensic science has become an integral tool for victim and perpetrator identifications, missing person’s cases, paternity testing, etc. A major use of DNA is in the identification of unknown deceased individuals. With a reported number of individuals well over 8,000 in the United States, improved methods to accurately collect and analyze DNA from modern human bone are needed.

This project took the preliminary steps to improve DNA sampling and extraction methods by analyzing the Y-chromosome DNA yield from the two bone types. While both types are composed of the same materials, cortical bone is the …

Reading The Bones: A Taphonomic Investigation Of Archaeofaunal Remains Recovered From Site 48pa551, Northwest Wyoming, Morgan H. Thurman

Graduate Student Theses, Dissertations, & Professional Papers

This is a preliminary taphonomy study of archaeofaunal remains found at site 48PA551, more commonly known as the Dead Indian Creek Site. 48PA551 is a well-known and commonly cited example of a McKean Complex occupation dating to between 3,800 and 4,800 B.P. The University of Montana held a field school at the site conducting small test excavations under the supervision of Dr. Anna Marie Prentiss in 2018. In the course of this testing a highly fragmentary bone bed consisting mostly of mammalian bone, cervids largely, was discovered eroding into the nearby creek. Ten 50 x 50 cm quadrants were excavated …

The Effects Of Heavy Metal Contamination In Soil On Dna Degradation After Decomposition, Samantha Hofland

Graduate Student Theses, Dissertations, & Professional Papers

The application of DNA is an ever-expanding field of research with many implications in the field of forensic investigation. Research into DNA obtained from soil is ongoing and suggests a new avenue in forensic investigation. This research seeks to understand the effects of heavy metal on the preservation of DNA within soil after decomposition of remains. Using pigs as a proxy for human remains, the effects of heavy metal contamination on the microbial environment and endogenous pig DNA was observed. This gave broad insight into the effect of surface burial and Western Montana; summer climate has on DNA degradation.

A Snapshot Of Care: Creating Models Of Care For Individuals Included In The Terry Collection, Felicia Robyn Sparozic

Graduate Student Theses, Dissertations, & Professional Papers

Utilizing the bioarchaeology of care model, this research formulates a model of care based on ethnohistorical and skeletal markers of pathologic conditions. The index of care is the method used to create models of care, and this requires social, cultural, economic, environmental, and mortuary contexts to determine the resources required to aid a person through injury or disability. This paper investigates the impact of known medical, occupational, and morgue records that accompanied each selected individual in the skeletal collection. This additional information, beyond what is gained from skeletal analysis, generates a complex model of care. A more complex model of …

A Comparative Analysis Of Homicide Rates Utilizing The University Of Tennessee Forensic Data Bank, Anna F. Hampton

Graduate Student Theses, Dissertations, & Professional Papers

Homicide has piqued the interest of society through media not only in the 21^st century, but also dating back to the 1800s with crime fiction novels. Homicide holds the attention of our society with crime drama television series and has become part of American culture. With the attention that homicide receives, studies focusing on homicide are therefore important. This research utilizes the University of Tennessee Forensic Data Bank (FDB) to examine and compare homicide rates to the Global Study on Homicide conducted by the United Nation Office on Drugs and Crime (UNODC). Variables examined include sex, ancestry, cause of …

Diet-Breadth Analysis In The American Southwest: Metabarcoding Method With Coprolites, Paige Nicole Plattner

Graduate Student Theses, Dissertations, & Professional Papers

Molecular analysis of coprolites is proposed as new means of studying bio-archaeological remains that aids in the ability to capture diet breadth from past populations. This research utilizes the targeted PCR method, known as metabarcoding, to study dietary elements preserved in the coprolites from three greater Southwest sites. The Illumina MiSeq technology was used to sequence the samples after multiple rounds of targeted PCR using two primers (16s and 12s) proven successful for taxonomic identification with animal DNA and one primer specific to plant DNA (Rias et al 2011).

The sequencing data was then run through two different bioinformatics pipelines …

Spirit Eye Cave: Reestablishing Provenience Of Trafficked Prehistoric Human Remains Using A Composite Collection-Based Ancient Dna Approach, Tre Blohm

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.