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Full-Text Articles in Animal Studies

Training The Concept Of Innovate In Dolphins (Tursiops Truncatus) Is Both Creative And Cognitively Stimulating, Deirdre Yeater, Kathleen M. Dudzinski, Dawn Melzer, Andrew R. Magee, Michaela Robinett, Gonzalo Guerra, Kimberly Salazar, Terri Bolton, Heather Manitzas Hill Jan 2024

Training The Concept Of Innovate In Dolphins (Tursiops Truncatus) Is Both Creative And Cognitively Stimulating, Deirdre Yeater, Kathleen M. Dudzinski, Dawn Melzer, Andrew R. Magee, Michaela Robinett, Gonzalo Guerra, Kimberly Salazar, Terri Bolton, Heather Manitzas Hill

Psychology Faculty Publications

Creative or novel behaviors in bottlenose dolphins (Tursiops truncatus) can be indicators of flexible thinking and problem solving. Over 50 years ago, two rough-tooth dolphins demonstrated creative novel behaviors acquired through reinforcement training in human care. Since this novel training, a variety of species have been trained to respond to this conceptual cue. The current study assessed the creativity of 12 bottlenose dolphins (5 females, 7 males) housed at the Roatan Institute for Marine Sciences (RIMS) in Roatan, Honduras. Individual differences were found across four constructs measured for creativity: fluency, flexibility, elaboration, and originality. Variability in performance occurred across test …


Mild Traumatic Brain Injury In Yucatan Minipigs: Assessing Changes In Approachability And Mechanical Nociception Following Diffuse Central Fluid Percussion Injury, Mark D. Pavlichenko Jan 2024

Mild Traumatic Brain Injury In Yucatan Minipigs: Assessing Changes In Approachability And Mechanical Nociception Following Diffuse Central Fluid Percussion Injury, Mark D. Pavlichenko

Theses and Dissertations

Traumatic brain injury (TBI) is a major healthcare concern affecting millions. Symptoms of TBI include sensory sensitivity and social changes that may persist long-term. Due to similarities in cytoarchitecture, metabolism, and inflammation, higher order minipig models are advantageous for translational TBI research, however, there remains a shortage of information regarding their behavioral and sensory sequelae following injury. Therefore, in this study, we assessed changes in sociability and somatosensation in adult male and female Yucatan minipigs for up to one-week following a sham or central fluid percussion injury (cFPI). Specifically, the voluntary human approach task (VHAT) and the forced human approach …


Multi-Year Effectiveness Of Pzp-22 In Free-Roaming Suburban White-Tailed Deer, Allen T. Rutberg, Kali M. Pereira, Kayla A. Grams, John W. Turner Jr. Jan 2024

Multi-Year Effectiveness Of Pzp-22 In Free-Roaming Suburban White-Tailed Deer, Allen T. Rutberg, Kali M. Pereira, Kayla A. Grams, John W. Turner Jr.

Human–Wildlife Interactions

To address the challenge that is posed to the use of fertility control to mitigate human–deer (Cervidae) conflicts by the need to regularly re-inoculate free-roaming animals, we captured, ear-tagged, and hand-injected 68 individual adult female white-tailed deer (Odocoileus virginianus) with the PZP-22 immunocontraceptive vaccine from February through April, 2014 to 2017, in the Village of Hastings-on-Hudson, New York, USA. Approximately 2.5 years after initial treatments, we booster-darted 29 of the previously treated females with either PZP-22 or native PZP (ZonaStat-D). We observed fawn production between 2014 and 2021. The combination of initial PZP-22 hand-treatments followed by single dart-delivered PZP …


The Auricular Surface Of Chimpanzees (Pan Troglodytes) As An Indicator Of Age, Kalila Garrison Jan 2024

The Auricular Surface Of Chimpanzees (Pan Troglodytes) As An Indicator Of Age, Kalila Garrison

All Master's Theses

Great ape skeletal remains are scarce and their use in biological profile estimations has been limited. Skeletal remains analysis provides key demographic information and ecological context to the individual. Chimpanzees (Pan troglodytes) share skeletal morphological characteristics with humans, and this study seeks to understand if two noninvasive methods using the auricular surface of the ilium to age human skeletal remains could be applied to chimpanzees. The sample consisted of n = 8 individual left auricular surfaces from known age groups of both sexes. Firstly, the Buckberry and Chamberlain Method assigns numerical values to five different traits on the auricular …


Social Interaction, Social Cognition, And Personality In Gartersnakes And Ball Pythons, Morgan Skinner Jan 2024

Social Interaction, Social Cognition, And Personality In Gartersnakes And Ball Pythons, Morgan Skinner

Theses and Dissertations (Comprehensive)

Snakes are often considered non-social animals. However, this is mostly an untested assumption and the limited research that has been done has shown that snakes interact socially when given the opportunity. Here, the complexity of snake social interactions was explored through a social cognition and personality lens. Group and individual behavioural assays were used to explore flexibility and consistency in social interactions. The results provide evidence that elucidate the function, development, and evolution of these behaviours in snakes. In the first experimental chapter, chapter 2, social network analysis was used to provide a detailed description of aggregation behaviour in 4 …


The Influence Of Anesthesia And Nicotine On Dopamine Signaling In The Rat Dorsal Striatum, Gracie E. Rosenbaum Jan 2024

The Influence Of Anesthesia And Nicotine On Dopamine Signaling In The Rat Dorsal Striatum, Gracie E. Rosenbaum

EWU Masters Thesis Collection

The striatum is highly innervated by dopamine neurons and is involved in movement control (dorsal striatum) reward learning (ventral striatum). Fast-scan cyclic voltammetry (FSCV) is a technique that uses microelectrodes (diameter ~5 μm, length ~150 μm) to monitor dopamine signaling in the brain. Although urethane and isoflurane anesthetics are commonly used in in vivo studies, isoflurane’s potential influence on dopamine signaling has not been thoroughly investigated. In addition to this, the addictive nature of nicotine indicates the importance of understanding its impact of dopamine neurotransmission within the different striatal subregions. Male Sprague-Dawley rats (Rattus norvegicus) 300 – 450 grams were …


Developing Resources To Foster Farmed Animal Agency In Sanctuary Education, Emily Tronetti Jan 2024

Developing Resources To Foster Farmed Animal Agency In Sanctuary Education, Emily Tronetti

Antioch University Dissertations & Theses

Farmed animal sanctuaries provide lifelong care to formerly farmed animals. Many strive to educate their community about farmed animals and promote more compassionate lifestyles, such as veganism. Important to this is cultivating empathy and concern for the well-being of individual farmed animals. Essential to well-being is agency, which is the capacity of a living being to engage with their environments and to make choices for themselves. Farmed animals outside of sanctuaries have had their agency systematically suppressed and denied. Sanctuary educators can bring awareness to this and inspire alternative, agency-centered relationships with not only farmed animals but all living beings. …


Living Among Wildlife: Elevating Human-Wildlife Interactions And Coexistence, Bridget Rebecca Murphy Dec 2023

Living Among Wildlife: Elevating Human-Wildlife Interactions And Coexistence, Bridget Rebecca Murphy

Graduate Student Portfolios, Professional Papers, and Capstone Projects

After a semester of learning, both in class and in nature, my writing honed in further on this human-nature divide. To me, I see humans as part of nature – as we are mammals, animals, part of the food chain, biological beings no higher than others on our planet. We have simply constructed this false narrative around us within our societies, minds and media that embeds this division between us and nature, between us and wildlife. Humans have been managing, stewarding, living off and within landscapes for thousands of years. As time and technology evolved, a lot of people began …


A Year Of Wins For Farmed Animals, Lewis Bollard Dec 2023

A Year Of Wins For Farmed Animals, Lewis Bollard

General – Farm Animal Issues

Although the European Union shelved its ambitious farmed animal welfare reforms, there were other “wins” for farmed animals in 2023. There were 130 new corporate pledges to eliminate cages for laying hens and the worst abuses of broiler chickens. So far, over 1,000 corporate pledges have been fully implemented, and 39% of American hens, 60% of European hens, and 80% of British hens are cage-free, up from 6%, 41%, and 48%, respectively, a decade ago. The US Supreme Court upheld California’s Proposition 12, which bans the sale of eggs, pork, and veal from caged animals and their offspring in California. …


A Year Of Wins For Farmed Animals, Lewis Bollard Dec 2023

A Year Of Wins For Farmed Animals, Lewis Bollard

Nexus – UNEP – Animal Welfare, Environment, Sustainable Development

Although the European Union shelved its ambitious farmed animal welfare reforms, there were other “wins” for farmed animals in 2023. There were 130 new corporate pledges to eliminate cages for laying hens and the worst abuses of broiler chickens. So far, over 1,000 corporate pledges have been fully implemented, and 39% of American hens, 60% of European hens, and 80% of British hens are cage-free, up from 6%, 41%, and 48%, respectively, a decade ago. The US Supreme Court upheld California’s Proposition 12, which bans the sale of eggs, pork, and veal from caged animals and their offspring in California. …


Using A Toxic Aging Coin To Assess Hexavalent Chromium-Induced Neurotoxicity., Samuel Thomas Vielee Dec 2023

Using A Toxic Aging Coin To Assess Hexavalent Chromium-Induced Neurotoxicity., Samuel Thomas Vielee

Electronic Theses and Dissertations

We are facing an aging crisis, with 20% of the U.S. population projected to be geriatric (65+) by 2030 and live another 40+ years. Age-related diseases accompany a growing geriatric population, emphasizing the need to understand their etiology. Environmental pollutants compound this crisis by: 1) geriatrics are more susceptible, exacerbating age-related diseases and comorbidities, and 2) they accelerate biological aging, inducing age-related diseases at younger ages. We address this crisis using a ‘toxic aging coin’ approach; heads examines how age impacts toxicity, tails examines how chemicals accelerate aging. This thesis applies the heads side for Cr(VI)-induced neurotoxicity across ages. We …


Habitat Associations Of Plains Spotted Skunk (Spilogale Interrupta) Across The Plains Ecoregion. And The Landcover Occupancy Models Of The Gray Fox (Urocyon Cinereoargenteus) In The Southeastern Plains Region In Arkansas, Michael Shaw Dec 2023

Habitat Associations Of Plains Spotted Skunk (Spilogale Interrupta) Across The Plains Ecoregion. And The Landcover Occupancy Models Of The Gray Fox (Urocyon Cinereoargenteus) In The Southeastern Plains Region In Arkansas, Michael Shaw

Graduate Theses and Dissertations

Much of the landscape of the United States has been altered by humans, leading to the eradication of most populations of large mammalian predators. Consequently, many species of mammalian mesopredators (medium-sized mammalian predators) have come to dominate the landscape. Species such as the gray fox (Urocyon cinereoargenteus) may appear to be on the rise and doing well due to their widespread range, but at a local scale may need monitoring and less is known in areas of its range. Others such as the plains spotted skunk have a wide range across the plains but are on the decline even with …


Demographics, Sexual Dimorphism, And Ecological Aspects Of Ambystoma Annulatum (Ringed Salamander) In Northwest Arkansas, Usa, Brian M. Becker Dec 2023

Demographics, Sexual Dimorphism, And Ecological Aspects Of Ambystoma Annulatum (Ringed Salamander) In Northwest Arkansas, Usa, Brian M. Becker

Graduate Theses and Dissertations

The need to study and monitor amphibian populations is increasing along with the threats to their population stability and persistence in nature. Northwest Arkansas is one of the fastest growing areas in the nation and with that growth comes rapid changes in land use, massive alterations to habitats, habitat loss, and the introduction of nonnative plants and animals. Ambystoma annulatum (Ringed Salamander) is an Ambystomatid endemic to the Ozark and Ouachita Mountains of southern Missouri, northern and western Arkansas, and eastern Oklahoma giving it a relatively small distribution compared to most Ambystoma. Therefore, Arkansas constitutes a significant portion of the …


Why Humans Kill Animals And Why We Cannot Avoid It, Benjamin L. Allen, Christopher Bobier, Stuart J. Dawson, Peter J S Fleming, Jordan Hampton, David Jachowski, Graham I H Kerley, John D. Linnell, Kelly Marnewick, Liaan Minnie, Mike Muthersbaugh, M Justin O'Riain, Dan Parker, Gilbert Proulx, Michael J. Somers, Keifer Titus Oct 2023

Why Humans Kill Animals And Why We Cannot Avoid It, Benjamin L. Allen, Christopher Bobier, Stuart J. Dawson, Peter J S Fleming, Jordan Hampton, David Jachowski, Graham I H Kerley, John D. Linnell, Kelly Marnewick, Liaan Minnie, Mike Muthersbaugh, M Justin O'Riain, Dan Parker, Gilbert Proulx, Michael J. Somers, Keifer Titus

Animal production and livestock research articles

Killing animals has been a ubiquitous human behaviour throughout history, yet it is becoming increasingly controversial and criticised in some parts of contemporary human society. Here we review 10 primary reasons why humans kill animals, discuss the necessity (or not) of these forms of killing, and describe the global ecological context for human killing of animals. Humans historically and currently kill animals either directly or indirectly for the following reasons: (1) wild harvest or food acquisition, (2) human health and safety, (3) agriculture and aquaculture, (4) urbanisation and industrialisation, (5) invasive, overabundant or nuisance wildlife control, (6) threatened species conservation …


Fauna As Fieldworkers: An Analysis On The Use Of Animals As A Form Of Organic Weed And Pest Management In Modern Viticulture, Mariana Del Carmen Campano Oct 2023

Fauna As Fieldworkers: An Analysis On The Use Of Animals As A Form Of Organic Weed And Pest Management In Modern Viticulture, Mariana Del Carmen Campano

Independent Study Project (ISP) Collection

Recent data on the current state of pesticide use in global agriculture suggests serious concerns over significant overuse and negative effects on both environmental and human health, and current trends only suggest continued increases in future global pesticide use. Without prompt, large-scale intervention, this global abuse of chemical pesticides spells significant threats of not only continued harm to global health and environmental quality, but also to the long term viability of agricultural lands and the growing threat of pesticide resistant insects and weeds. Various methods have been explored in the realm of integrated pest management (IPM), but a current lack …


Analyzing Human - Nonhuman Primate Conflict Mitigation Techniques In Mto Wa Mbu, Northern Tanzania, Lily Adams Oct 2023

Analyzing Human - Nonhuman Primate Conflict Mitigation Techniques In Mto Wa Mbu, Northern Tanzania, Lily Adams

Independent Study Project (ISP) Collection

Human – non-human primate conflict is particularly crucial due to primates’ high capacity to live among human populations. The study to analyze Human - Nonhuman Primate Conflict Mitigation Techniques, was carried in November 2023 at Mto wa Mbu, Northern Tanzania. To analyze techniques currently being used to mitigate human – non-human primate conflict, this study collected data through semi-structured interviews. Over 87% (n=35) of respondents used multiple mitigation techniques simultaneously. 80% of respondents (n=32) reported using projectiles to ward off foraging primates, 75% of respondents (n=30) reported using loud noises (made either by the voice/body or by manipulating noisemakers), 67.5% …


Creating A Framework For An Effective Koala Recovery Center: A Case Study Of Friends Of The Koala, Nsw, Finley Scoular Oct 2023

Creating A Framework For An Effective Koala Recovery Center: A Case Study Of Friends Of The Koala, Nsw, Finley Scoular

Independent Study Project (ISP) Collection

Koala populations in New South Wales, Queensland, and the Australian Capital District are currently faced with factors that threaten their survival. Within one decade, their status has shifted from stable to vulnerable to endangered. The cause of this is attributed to increased exposure to dog attacks, vehicle collisions, diseases, and habitat fragmentation and destruction. The koalas' status of endangered in these territories has illuminated the need for human intervention. This study focuses on the specific form of human intervention that involves the rescue, rehabilitation, and release of wild koalas. Due to the recent koala status change from vulnerable to endangered, …


Við Pollurinn: The Effects Of Human-Caused Stimuli On Harbor Seals (Phoca Vitulina) In Ísafjörður, Ella Jorgensen Oct 2023

Við Pollurinn: The Effects Of Human-Caused Stimuli On Harbor Seals (Phoca Vitulina) In Ísafjörður, Ella Jorgensen

Independent Study Project (ISP) Collection

Harbor seals are important for the health of Iceland’s marine ecosystems. They are normally seen hauled-out onto land to rest. There are many factors that affect the haul-out patterns of harbor seals including location, season, time of day, tide height, weather, and human-caused stimuli. The presence of human-caused stimuli can also have a negative impact on haul-out patterns and behaviors. The Icelandic harbor seal population is threatened as it has been declining since 1980. Climate change and the rise of tourism in Iceland may be a factor contributing to this decline; and there is a gap in knowledge on the …


Investigating Monodelphis Domestica As An Alternative To The Mus Musculus As An Animal Model, Cristian M. Botello, John L. Vandeberg, Mario Gil Sep 2023

Investigating Monodelphis Domestica As An Alternative To The Mus Musculus As An Animal Model, Cristian M. Botello, John L. Vandeberg, Mario Gil

Research Symposium

Background: Mus Musculus is one of the first and one of the most widely used animal models in current neuroscience literature (Phifer-Riley & Nachmann, 2015). However, the research community needs alternatives to rodent models to study the mammalian brain. Research is needed to see if antibodies that target tyrosine hydroxylase, which are well researched in mice, can also be used to study the Monodelphis domestica brain.

Methods: Following transcardial perfusions and brain extractions, mouse and opossum brains were processed and stained for tyrosine hydroxylase (and with Nissl). Opossum brains will then be sliced and processed using IHC methods to compare …


Evaluation Of Heat Abatement Use, Vaginal Temperature, And Activity Of Pregnant, Lactating Holstein Cows Housed On Pasture With Or Without The Choice Between Shade And Sprinklers For Heat Abatement., Kevin M. Braman Aug 2023

Evaluation Of Heat Abatement Use, Vaginal Temperature, And Activity Of Pregnant, Lactating Holstein Cows Housed On Pasture With Or Without The Choice Between Shade And Sprinklers For Heat Abatement., Kevin M. Braman

Theses and Dissertations

The objective of this thesis was to determine how pregnant, lactating Holstein cows on pasture interact with shade and sprinklers when offered a choice or no-choice between methods. Authors hypothesized that cows would use shade more then sprinklers, but sprinklers would be more effective at cooling cows. Additionally, authors predicted cows using shade more would have decreased lying bouts and steps and an increase in time lying, compared to cows choosing sprinkler. Results indicate cows will use both methods when offered alone or together. However, cows will choose to be exposed or in shade at lower temperature humidity index values, …


A Four-Pronged Approach To Addressing A Wild Pig Invasion In A Bottomland And Upland Forested Landscape, Tyler Scott Evans Aug 2023

A Four-Pronged Approach To Addressing A Wild Pig Invasion In A Bottomland And Upland Forested Landscape, Tyler Scott Evans

Theses and Dissertations

Among exotic species that are capable of invading, establishing, and reaching pest status, few pose the range of impacts to biotic (e.g., competition with native species, predation, herbivory, introduction of other exotics) and abiotic (e.g., soil, hydrology) ecosystem components that can be attributed to the wild pig (Sus scrofa). Despite the presence of wild pigs throughout the southeastern United States for centuries, new invasions continue to occur in previously uninhabited and often under-investigated landscapes, including bottomland and upland forests. The recent invasion of the Sam D. Hamilton Noxubee National Wildlife Refuge (hereafter, NNWR) in east-central Mississippi represents an …


Shark-Diving Tourism In The Macaronesian Archipelagos: Challenges And Opportunities, Pedro Gonzáles Mantilla Jun 2023

Shark-Diving Tourism In The Macaronesian Archipelagos: Challenges And Opportunities, Pedro Gonzáles Mantilla

ITSA 2022 Gran Canaria - 9th Biennial Conference: Corporate Entrepreneurship and Global Tourism Strategies After Covid 19

The Macaronesian region is typically heavily exploited by fisheries; however, in recent years, marine wildlife tourism has become popular and a shark-diving industry has emerged, potentially presenting an alternative for the sustainable use of sharks. Combining a literature review with interviews with dive operators conducting shark encounters in the Macaronesian archipelagos, we provide an overview of the challenges and conservation potential of shark-diving tourism for these territories. Owing to the regular presence of important shark species for tourism and the growth of the scuba-diving industry, shark-diving has potential to expand over the region. Yet, the overlap between European industrial fishing …


Statistical And Biological Analyses Of Acoustic Signals In Estrildid Finches, Moises Rivera Jun 2023

Statistical And Biological Analyses Of Acoustic Signals In Estrildid Finches, Moises Rivera

Dissertations, Theses, and Capstone Projects

Acoustic communication is a process that involves auditory perception and signal processing. Discrimination and recognition further require cognitive processes and supporting mechanisms in order to successfully identify and appropriately respond to signal senders. Although acoustic communication is common across birds, classical research has largely disregarded the perceptual abilities of perinatal altricial taxa. Chapter 1 reviews the literature of perinatal acoustic stimulation in birds, highlighting the disproportionate focus on precocial birds (e.g., chickens, ducks, quails). The long-held belief that altricial birds were incapable of acoustic perception in ovo was only recently overturned, as researchers began to find behavioral and physiological evidence …


Towards Urban Biodiversity: Simulating Design Parameters For Wildlife-Inclusive Green Infrastrcuture, Alisa Abdulghany, Baher Farahat May 2023

Towards Urban Biodiversity: Simulating Design Parameters For Wildlife-Inclusive Green Infrastrcuture, Alisa Abdulghany, Baher Farahat

BAU Journal - Creative Sustainable Development

As of 2021, more than half of the global population resides in urban areas. This resulted in an overwhelming footprint affecting species habitat areas leading to biodiversity loss. By definition, urban biodiversity is the diversity of living things within the urban realm. By providing chances for habitat as part of new developments, preserving on-site habitats, and attempting to connect with the local ecosystems, we can help promote biodiversity. Green infrastructure (GI), which includes all semi-natural areas in the urban context, can serve as a vessel for biodiversity. Therefore, the problem can be defined by the urban footprint that expands on …


Gpr75 Deficiency Attenuates High Fat Diet-Driven Obesity And Glucose Intolerance, Sakib Hossain May 2023

Gpr75 Deficiency Attenuates High Fat Diet-Driven Obesity And Glucose Intolerance, Sakib Hossain

NYMC Student Theses and Dissertations

Recently, a collaboration between Regeneron Pharmaceuticals and the Schwartzman-Garcia labs at New York Medical College published an exome sequencing study of individuals across the United Kingdom, United States, and Mexico which concluded that individuals possessing non-functioning, truncated mutations to the orphan g protein coupled receptor (GPCR), GPR75, had lower BMI and 54% reduced likelihood of obesity. The present study was undertaken to fully characterize the metabolic phenotype of Gpr75 deficient mice when fed a high fat diet (HFD) and explore potential mechanisms by which GPR75 activation links to increased adiposity and decreased glucose tolerance. After 14 weeks of HFD-feeding we …


Associative Learning: Unmet Criterion For Plant Sentience, Luigi Baciadonna, Catherine Macri, Martin Giurfa May 2023

Associative Learning: Unmet Criterion For Plant Sentience, Luigi Baciadonna, Catherine Macri, Martin Giurfa

Animal Sentience

In a thought-provoking target article, Segundo-Ortin & Calvo (S&C) discuss the possibility that plants are sentient, focusing on a series of capacities normally attributed only to human and nonhuman animals. S&C propose learning as a marker for sentience. We review studies reporting associative learning in plants and find that they either lack essential controls or fail to produce replicable results. The capacity to learn has not yet been demonstrated in plants, so it cannot be used to support the hypothesis that plants are sentient. Further studies are needed. But agnosticism about sentience should not deter us from investigating unexpected new …


Humanity In Animals: A Exploration Of The Complexity Of Elephants As Intellectual Beings, Xing Z. Huang May 2023

Humanity In Animals: A Exploration Of The Complexity Of Elephants As Intellectual Beings, Xing Z. Huang

Honors Projects

Loxodonta (Elephants) are endangered species with only around 40,000-50,000 left in the world. They are part of the Elephantidae family with only three known existing species. Known for their cognitive capacity in their ability to recognize themselves in a mirror among many other actions, such as using self-made tools to aid them in their daily lives. Through past studies, it can be seen that vocalization and physical touch are the key steps of communication. Elephants have demonstrated their ability to distinguish between different frequencies and utilize them to communicate changes in the environment or indicate the absence of a herd …


A Deeper Understanding Of Noise Effects On Cetaceans, Jason N. Bruck May 2023

A Deeper Understanding Of Noise Effects On Cetaceans, Jason N. Bruck

Faculty Publications

Recent research with cetaceans under human care is illuminating just how dolphins are affected by human-made noise both in terms of their ability to cooperate as well as their ability to habituate to such noise. This research is providing granular detail to regulators assessing the problems associated with anthropogenic effects and is highlighting a role for behavior/cognition research in conservation.


Neuronal Effects Of Cocaine In An Animal Model Of Social Stress: Analysis Of Neuronal Recordings, Eboni Eddins May 2023

Neuronal Effects Of Cocaine In An Animal Model Of Social Stress: Analysis Of Neuronal Recordings, Eboni Eddins

Honors Theses

Studies that use Intermittent (episodic) Social Defeat (ISD) in rats demonstrate that ISD increases cocaine-self administration several weeks after the end of the adverse experience and suggest that a history of social stress makes individuals more vulnerable to substance abuse in the long term. The medial prefrontal cortex (mPFC) plays a key role in regulating drug-seeking behavior. The present study investigates whether ISD enhances the response of mPFC neurons to cocaine. Male Long Evans rats (3-4 months) were implanted with electrode arrays in the mPFC (prelimbic area) and divided into two groups (Control, n= 4; Stress, n= 4). They were …


An Ecological Perspective Of American Rodent-Borne Orthohantavirus Surveillance, Nathaniel Mull May 2023

An Ecological Perspective Of American Rodent-Borne Orthohantavirus Surveillance, Nathaniel Mull

Graduate Theses and Dissertations

Orthohantaviruses are a global group of viruses found primarily in rodents, though several viruses have also been found in shrews and moles. Many rodent-borne orthohantaviruses are capable of causing one of several diseases in humans, and the mortality associated with these diseases ranges from < 0.1% - 50% depending on the specific etiological virus. In North and South America, orthohantavirus research was ignited by an outbreak of severe disease in the Four Corners region of the United States in 1993. However, despite the discovery of over 20 orthohantaviruses in the Americas, our understanding of orthohantavirus ecology and virus-host dynamics in this region is still limited, and orthohantavirus surveillance is generally restricted in scope to select regions and small portions of host distributional ranges. In Chapter I, I present a literature review on the current understanding of American rodent-borne orthohantavirus ecology. This review focused on under-studied orthohantaviruses, addressing gaps in knowledge by extrapolating information from well-studied orthohantaviruses, general rodent ecology, and occassionally from Eurasian orthohantavirus-host ecology. There were several key conclusions generated from this review that warrant further research: 1) the large number of putative orthohantaviruses and gaps in orthohantavirus evolution necessitate further surveillance and characterization, 2) orthohantavirus traits differ and are more generalizable based on host taxonomy rather than geography, and 3) orthohantavirus host species are disproportionately found in grasslands and disturbed habitats. In Chapter II, I present a prioritized list of rodent species to target for orthohantavirus surveillance based on predictive modeling using machine learning. Probable orthohantavirus hosts were predicted based on traits of known orthohantavirus hosts using two different types of evidence: RT-PCR and virus isolation. Predicted host distributions were also mapped to identify geographic hotspots to spatially guide future surveillance efforts. In Chapter III, I present a framework for understanding and predicting orthohantavirus traits based on reservoir host phylogeny, as opposed to the traditional geographic dichotomy used to group orthohantaviruses. This framework establishes three distinct orthohantavirus groups: murid-borne orthohantaviruses, arvicoline-borne orthohantaviruses, and non-arvicoline cricetid-borne orthohantaviruses, which differ in several key traits, including the human disease they cause, transmission routes, and virus-host fidelity. In Chapter IV, I compare rodent communities and orthohantavirus prevalence among grassland management regimes. Sites that were periodically burned had high rodent diversity and a high proportion of grassland species. However, rodent seroprevalence for orthohantavirus was also highest in burned sites, representing a trade-off in habitat management outcomes. The high seroprevalence in burned sites is likely due to the robust populations supported by the high quality habitat resulting from prescribed burning. In Chapters V and VI, I describe Ozark virus and Sager Creek virus, two novel orthohantaviruses discovered from specimens collected during Chapter IV. Both chapters report full genome sequences of the respective viruses and compare both nucleotide and protein phylogenies with related orthohantaviruses. Additionally in Chapter VI, I support the genetic analyses with molecular and ecological characterizations, including seasonal fluctuations in host abundance, correlates of prevalence, evidence of virus shedding, and information on host cell susceptibility to Sager Creek virus.