Open Access. Powered by Scholars. Published by Universities.®

Optics Commons™

Open Access. Powered by Scholars. Published by Universities.®

Discipline
Institution
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 811 - 840 of 2316

Full-Text Articles in Optics

Combined High-Speed Single Particle Tracking Of Membrane Proteins And Super-Resolution Of Membrane-Associated Structures, Hanieh Mazloom Farsibaf, Keith A. Lidke Nov 2018

Combined High-Speed Single Particle Tracking Of Membrane Proteins And Super-Resolution Of Membrane-Associated Structures, Hanieh Mazloom Farsibaf, Keith A. Lidke

Shared Knowledge Conference

Many experiments have shown that the diffusive motion of lipids and membrane proteins are slower on the cell surface than those in artificial lipid bilayers or blebs. One hypothesis that may partially explain this mystery is the effect of the cytoskeleton structures on the protein dynamics. A model proposed by Kusumi [1] is the Fence-Picket Model which describes the cell membrane as a set of compartment regions, each ~ 10 to 200 nm in size, created by direct or indirect interaction of lipids and proteins with actin filaments just below the membrane. To test this hypothesis, we have assembled a …


Charge Transfer Plasmon Resonances In Metallic Nanorod-Film Systems, Paul J. Gieri Nov 2018

Charge Transfer Plasmon Resonances In Metallic Nanorod-Film Systems, Paul J. Gieri

Shared Knowledge Conference

Understanding how the plasmonic response of colloidally grown metallic nanostructures changes when coupled to a metallic film is an important research problem with significant consequences for a number of applications such as sensing, solar energy harvesting, spectroscopy, and photochemistry, to name a few. In this work we investigate, both through experimental and theoretical approaches, the optical response of ligand coated gold nanorods and their interaction with gold films. We find that the scattering response of these systems is dominated by a charge transfer plasmon, in which charge flows between the particle and film. Additionally, we show that the characteristics of …


Pulsed Fiber Optics Lasers As Highly Sensitive Sensors, Hanieh Afkhamiardakani Nov 2018

Pulsed Fiber Optics Lasers As Highly Sensitive Sensors, Hanieh Afkhamiardakani

Shared Knowledge Conference

An interferometer or resonator is a device in which optical beams of specific frequencies circulate with minimal losses. These losses are completely compensated by the gain inside a laser resonator. A small perturbation introduced inside the laser can affect its frequency, which in turns becomes a metric of that perturbation. The perturbation is usually caused by an electric or magnetic field, rotation, acceleration, nonlinear index of refraction etc. Tiny changes of optical frequency are monitored by superimposing the laser field and a reference field (from the same laser) on a detector. This technique requires creating a laser in which two …


Effects Of Fabrication Errors On The Focusing Performance Of A Sector Metalens, S. S. Stafeev, A. G. Nalimov, Liam O’Faolain, M. V. Kotlyar Nov 2018

Effects Of Fabrication Errors On The Focusing Performance Of A Sector Metalens, S. S. Stafeev, A. G. Nalimov, Liam O’Faolain, M. V. Kotlyar

Cappa Publications

Using e-beam lithography, a 16-sector spiral metalens was fabricated in an amorphous silicon, capable of converting linearly polarized incident light into an azimuthally polarized optical vortex. When illuminated by a 633-nm linearly polarized laser beam, the metalens generated a near-surface subwavelength focal spot equal to 0.75 of the incident wavelength at full-width of half-maximum intensity. The focusing performance of the spiral metalens was numerically shown to be sensitive to the deviation of the factual microrelief from the calculated height. For the designed microrelief height, a circularly polarized incident beam was focused into a bright ring with a reverse energy flow …


Rainbow Horizons: High Altitude Visible Spectrum Analysis, F Gonzalez, N Schragal, E Leiser, A Raney, J Seese Oct 2018

Rainbow Horizons: High Altitude Visible Spectrum Analysis, F Gonzalez, N Schragal, E Leiser, A Raney, J Seese

Von Braun Symposium Student Posters

No abstract provided.


Beam Theory For Classical And Quantum Nonlinear Optics---Exposing Classical And Quantum Correlations Of Transverse-Spatial Modes, Robert Nicholas Lanning Oct 2018

Beam Theory For Classical And Quantum Nonlinear Optics---Exposing Classical And Quantum Correlations Of Transverse-Spatial Modes, Robert Nicholas Lanning

LSU Doctoral Dissertations

Optics is arguably the most important branch of physics that has ever been studied. It is not only an essential ingredient of many other branches of physics that we study, it governs how we see, how we measure, and how we communicate in the modern world. And as the world continues to change, so do our tools and resources. In a relatively short amount of time, we have progressed from rudimentary tools that shape the world around us, to tools that harness the fundamental laws of nature. Unsurprisingly, the laws of nature governing optics remain paramount. This is because many …


Indirect Imaging Using Computational Imaging Techniques, Aparna Viswanath Oct 2018

Indirect Imaging Using Computational Imaging Techniques, Aparna Viswanath

Electrical Engineering Theses and Dissertations

The work describes various methods employed towards solving the problem of indirect imaging. Computational techniques are employed to indirectly decipher information about an object hidden from view of a camera. Notion of virtualizing the source of illumination and detectors on real world rough surfaces was exploited to construct a non line of sight computational imager. Diversity was explored from the stand point of both illumination of the object and imaging of light reflected from the object. To understand the impact of scattering by real world rough surfaces, an instrument was developed that allows characterization of isoplanatic angle for different surface …


Design And Optimization Of A 3-D Plasmonic Huygens Metasurface For Highly-Efficient Flat Optics, Bryan M. Adomanis Sep 2018

Design And Optimization Of A 3-D Plasmonic Huygens Metasurface For Highly-Efficient Flat Optics, Bryan M. Adomanis

Theses and Dissertations

For miniaturization of future USAF unmanned aerial and space systems to become feasible, accompanying sensor components of these systems must also be reduced in size, weight and power (SWaP). Metasurfaces can act as planar equivalents to bulk optics, and thus possess a high potential to meet these low-SWaP requirements. However, functional efficiencies of plasmonic metasurface architectures have been too low for practical application in the infrared (IR) regime. Huygens-like forward-scattering inclusions may provide a solution to this deficiency, but there is no academic consensus on an optimal plasmonic architecture for obtaining efficient phase control at high frequencies. This dissertation asks …


Efficiency Enhancement Of Perovskite Solar Cells With Plasmonic Nanoparticles: A Simulation Study, Ali Hajjiah, Ishac Kandas, Nader Shehata Sep 2018

Efficiency Enhancement Of Perovskite Solar Cells With Plasmonic Nanoparticles: A Simulation Study, Ali Hajjiah, Ishac Kandas, Nader Shehata

Biology Faculty Publications

Recently, hybrid organic-inorganic perovskites have been extensively studied due to their promising optical properties with relatively low-cost and simple processing. However, the perovskite solar cells have some low optical absorption in the visible spectrum, especially around the red region. In this paper, an improvement of perovskite solar cell efficiency is studied via simulations through adding plasmonic nanoparticles (NPs) at the rear side of the solar cell. The plasmonic resonance wavelength is selected to be very close to the spectrum range of lower absorption of the perovskite: around 600 nm. Both gold and silver nanoparticles (Au and Ag NPs) are selected …


Reconstruction Of The 3d Temperature And Species Concentration Spatial Distribution Of A Jet Engine Exhaust Plume Using An Infrared Fourier Transform Spectrometer Hyperspectral Imager, Mason D. Paulec Sep 2018

Reconstruction Of The 3d Temperature And Species Concentration Spatial Distribution Of A Jet Engine Exhaust Plume Using An Infrared Fourier Transform Spectrometer Hyperspectral Imager, Mason D. Paulec

Theses and Dissertations

The measurement of combustion byproducts is useful for determining pollution of any fuel burning application, efficiency of combustion, and determining detectability of aircraft exhausts. Both intrusive and non-intrusive techniques have been utilized to measure these quantities. For the majority of the non-intrusive techniques, the absorption and emission spectra of the gases are utilized for measurements. For this research, the use of the Telops Infrared Fourier Transform Spectrometer (IFTS) Hyperspectral Imager (HSI) was explored within the scope of combustion diagnostic methods, as an option for remote measurements of a jet turbine to determine concentration of species and temperature of the combustion …


Catalysis Of Stark-Tuned Interactions Between Ultracold Rydberg Atoms, A. L. Win, W. D. Williams, T. J. Carroll, C. I. Sukenik Sep 2018

Catalysis Of Stark-Tuned Interactions Between Ultracold Rydberg Atoms, A. L. Win, W. D. Williams, T. J. Carroll, C. I. Sukenik

Physics Faculty Publications

We have experimentally investigated a catalysis effect in the resonant energy transfer between ultracold 85Rb Rydberg atoms. We studied the time dependence of the process, 34p + 34p → 34s + 35s, and observed an enhancement of 34s state population when 34d state atoms are added. We have also performed numerical model simulations, which are in qualitative agreement with experiment and indicate that the enhancement arises from a redistribution of p-state atoms due to the presence of the d-state atoms.


Charge State Dynamics And Quantum Sensing With Defects In Diamond, Jacob D. Henshaw Sep 2018

Charge State Dynamics And Quantum Sensing With Defects In Diamond, Jacob D. Henshaw

Dissertations, Theses, and Capstone Projects

In recent years, defect centers in wide band gap semiconductors such as diamond, have received significant attention. Defects offer great utility as single photon emitters, nanoscale sensors, and quantum memories and registers for quantum computation. Critical to the utility of these defects, is their charge state.

In this dissertation, experiments surrounding the charge state dynamics and the carrier dynamics are performed and analyzed. Extensive studies of the ionization and recombination processes of defects in diamond, specifically, the Nitrogen Vacancy (NV) center, have been performed. Diffusion of ionized charge carriers has been imaged indirectly through the recapture of said carriers by …


Evaluation And Quantification Of Diffractive Plenoptic Camera Algorithm Performance, Jack A. Shepherd Iii Sep 2018

Evaluation And Quantification Of Diffractive Plenoptic Camera Algorithm Performance, Jack A. Shepherd Iii

Theses and Dissertations

A diffractive plenoptic camera is a novel approach to the traditional plenoptic camera which replaces the main optic with a Fresnel zone plate making the camera sensitive to wavelength instead of range. However, algorithms are necessary to reconstruct the image produced by plenoptic cameras. While many algorithms exist for traditional plenoptic cameras, their ability to create spectral images in a diffractive plenoptic camera is unknown. This paper evaluates digital refocusing, super resolution, and 3D deconvolution through a Richardson-Lucy algorithm as well as a new Gaussian smoothing algorithm. All of the algorithms worked well near the Fresnel zone plate design wavelength, …


Techniques For Improved Space Object Detection Performance From Ground-Based Telescope Systems Using Long And Short Exposure Images, David J. Becker Aug 2018

Techniques For Improved Space Object Detection Performance From Ground-Based Telescope Systems Using Long And Short Exposure Images, David J. Becker

Theses and Dissertations

Space object detection is of great importance in the highly dependent yet competitive and congested space domain. Detection algorithms employed play a crucial role in fulfilling the detection component in the space situational awareness mission to detect, track, characterize and catalog unknown space objects. Many current space detection algorithms use a matched filter or a spatial correlator on long exposure data to make a detection decision at a single pixel point of a spatial image based on the assumption that the data follows a Gaussian distribution. This research focuses on improving current space object detection algorithms and developing new algorithms …


Compressive Direct Imaging Of A Billion-Dimensional Optical Phase Space, Samuel H. Knarr, Daniel J. Lum, James Schneeloch, John C. Howell Aug 2018

Compressive Direct Imaging Of A Billion-Dimensional Optical Phase Space, Samuel H. Knarr, Daniel J. Lum, James Schneeloch, John C. Howell

Mathematics, Physics, and Computer Science Faculty Articles and Research

Optical phase spaces represent fields of any spatial coherence and are typically measured through phase-retrieval methods involving a computational inversion, optical interference, or a resolution-limiting lenslet array. Recently, a weak-values technique demonstrated that a beam's Dirac phase space is proportional to the measurable complex weak value, regardless of coherence. These direct measurements require raster scanning through all position-polarization couplings, limiting their dimensionality to less than 100 000 [C. Bamber and J. S. Lundeen, Phys. Rev. Lett. 112, 070405 (2014)]. We circumvent these limitations using compressive sensing, a numerical protocol that allows us to undersample, yet efficiently measure, high-dimensional phase spaces. …


In Vivo Vascular Imaging With Photoacoustic Microscopy, Hsun-Chia Hsu Aug 2018

In Vivo Vascular Imaging With Photoacoustic Microscopy, Hsun-Chia Hsu

McKelvey School of Engineering Graduate Student Theses & Dissertations

Photoacoustic (PA) tomography (PAT) has received extensive attention in the last decade for its capability to provide label-free structural and functional imaging in biological tissue with highly scalable spatial resolution and penetration depth. Compared to modern optical modalities, PAT offers speckle-free images and is more sensitive to optical absorption contrast (with 100% relative sensitivity). By implementing different regimes of optical wavelength, PAT can be used to image diverse light-absorbing biomolecules. For example, hemoglobin is of particular interest in the visible wavelength regime owing to its dominant absorption, and lipids and water are more commonly studied in the near-infrared regime.

In …


Automating Mobile Device File Format Analysis, Richard A. Dill Aug 2018

Automating Mobile Device File Format Analysis, Richard A. Dill

Theses and Dissertations

Forensic tools assist examiners in extracting evidence from application files from mobile devices. If the file format for the file of interest is known, this process is straightforward, otherwise it requires the examiner to manually reverse engineer the data structures resident in the file. This research presents the Automated Data Structure Slayer (ADSS), which automates the process to reverse engineer unknown file for- mats of Android applications. After statically parsing and preparing an application, ADSS dynamically runs it, injecting hooks at selected methods to uncover the data structures used to store and process data before writing to media. The resultant …


Room Temperature Operation Of Quantum Cascade Lasers Monolithically Integrated Onto A Lattice-Mismatched Substrate, Rowel Go Aug 2018

Room Temperature Operation Of Quantum Cascade Lasers Monolithically Integrated Onto A Lattice-Mismatched Substrate, Rowel Go

Electronic Theses and Dissertations

Quantum Cascade Lasers (QCLs) are semiconductor devices that, currently, have been observed to emit radiation from ~ 2.6 μm to 250 μm (1 to 100 terahertz range of frequencies.) They have established themselves as the laser of choice for spectroscopic gas sensing in the mid-wavelength infrared (3-8 μm) and long-wavelength infrared (8-15 μm) region. In the 4-12 μm wavelength region, the highest performing QCL devices, in terms of wall-plug efficiency and continuous wave operation, are indium phosphide (InP) based. The ultimate goal is to incorporate this InP-based QCL technology to silicon (Si) substrate since most opto-electronics are Si-based. The main …


A System For Conducting Laser-Induced Fluorescence Measurements On Gas Mixtures Exposed To Alpha Radiation, Patrick Ables Aug 2018

A System For Conducting Laser-Induced Fluorescence Measurements On Gas Mixtures Exposed To Alpha Radiation, Patrick Ables

Master's Theses

This paper documents modifications to an existing vacuum system to allow laser-induced fluorescence spectroscopy measurements within simulated atmospheres under a variety of conditions. This added capability will expand the laboratory’s ability to experimentally validate a computational model that calculates the effects of radiation within the atmosphere. The computational model could reveal radiation-induced chemical products that can be used to develop an alternative detection method that can be implemented from a safe distance. The selection of molecules for experimental validation has been limited to those which can be detected utilizing cavity ringdown spectroscopy. The current model indicates nitric oxide and ozone …


Compensation Of Non-Linear Bandwidth Broadening By Laser Chirping In Thomson Sources, C. Maroli, V. Petrillo, I. Drebot, L, Serafini, B. Terzić, G. A. Krafft Aug 2018

Compensation Of Non-Linear Bandwidth Broadening By Laser Chirping In Thomson Sources, C. Maroli, V. Petrillo, I. Drebot, L, Serafini, B. Terzić, G. A. Krafft

Physics Faculty Publications

A new laser chirping prescription is derived by means of the phase-stationary method for an inci- dent Gaussian laser pulse in conjunction with a Li enard-Wiechert calculation of the scattered radia- tion flux and spectral brilliance. This particularly efficient laser chirp has been obtained using the electric field of the laser and for electrons and radiation on axis. The frequency modulation is some- what reduced with respect to that proposed in the previous literature, allowing the application of this procedure to lasers with larger values of the parameter a0. Numerical calculations have been performed using mildly focused and …


Scattering Of Few Photon Fields By Two Level Systems In A One Dimensional Geometry, William Konyk Aug 2018

Scattering Of Few Photon Fields By Two Level Systems In A One Dimensional Geometry, William Konyk

Graduate Theses and Dissertations

Recent experimental progress has realized strong, efficient coupling of effective two level systems to waveguides. We study the scattering of multimode photons from such emitters coupled losslessly to the confined geometry of a one dimensional waveguide. We develop novel techniques for describing the scattered state of both single and multi-photon wavepackets and explore how such wavepackets interact with arrays of emitters coupled to a one dimensional waveguide. Finally, we apply these techniques and analyze the capability of two particular systems to act as a quantum conditional logic gate.


Thermally Stable Hybrid Cavity Laser Based On Silicon Nitride Gratings, Simone Iadanza, Andrei P. Bakoz, Praveen K. J. Singaravelu, Danilo Panettieri, Stefan Schulz, Ganga Chinna Rao Devarapu, Sylvain Guerber, Charles Baudot, Frédéric Boeuf, Stephen Hegarty, Liam O'Faolain Jul 2018

Thermally Stable Hybrid Cavity Laser Based On Silicon Nitride Gratings, Simone Iadanza, Andrei P. Bakoz, Praveen K. J. Singaravelu, Danilo Panettieri, Stefan Schulz, Ganga Chinna Rao Devarapu, Sylvain Guerber, Charles Baudot, Frédéric Boeuf, Stephen Hegarty, Liam O'Faolain

Cappa Publications

In this paper, we show the experimental results of a thermally stable Si3N4 external cavity (SiN EC) laser with high power output and the lowest SiN EC laser threshold to our knowledge. The device consists of a 250 μm sized reflective semiconductor optical amplifier butt-coupled to a passive chip based on a series of Si3N4 Bragg gratings acting as narrow reflectors. A threshold of 12 mA has been achieved, with a typical side-mode suppression ratio of 45 dB and measured power output higher than 3 mW. Furthermore, we achieved a mode-hop free-lasing regime in the range of 15–62 mA and …


Investigation Of Spin And Dip-Coating Phase Change Chalcogenide Materials As A Novel Technique For Coating And Functionalizing Conformal Optics, Paul Vecchio Jul 2018

Investigation Of Spin And Dip-Coating Phase Change Chalcogenide Materials As A Novel Technique For Coating And Functionalizing Conformal Optics, Paul Vecchio

Physics and Astronomy Summer Fellows

Chalcogenide glasses (ChGs) have excellent infrared (IR) transparency ranging up to 20 μm, large nonlinear refractive indices, and tailorable thermo-optic coefficients (dn/dT) that have great potential for applications in detectors, sensors, waveguides, imaging devices, photonic waveguides, acousto-optics, and optical devices. Spin and dip coating ChGs from solution is a low-cost, simple, and scalable method for depositing films over a large area. In this study, we utilize the novel approach of spin and dip coating to create thin ChG films of arsenic trisulfide (As2S3) and arsenic selenide (As2Se3). To realize the full potential …


Nanostructured Fibers As A Versatile Photonic Platform: Radiative Cooling And Waveguiding Through Transverse Anderson Localization, Norman Nan Shi, Cheng-Chia Tsai, Michael J. Carter, Jyotirmoy Mandal, Adam C. Overvig, Matthew Y. Sfeir, Ming Lu, Catherine L. Craig, Gary D. Bernard, Yuan Yang, Nanfang Yu Jul 2018

Nanostructured Fibers As A Versatile Photonic Platform: Radiative Cooling And Waveguiding Through Transverse Anderson Localization, Norman Nan Shi, Cheng-Chia Tsai, Michael J. Carter, Jyotirmoy Mandal, Adam C. Overvig, Matthew Y. Sfeir, Ming Lu, Catherine L. Craig, Gary D. Bernard, Yuan Yang, Nanfang Yu

Publications and Research

Broadband high reflectance in nature is often the result of randomly, three-dimensionally structured materials. This study explores unique optical properties associated with one-dimensional nanostructures discovered in silk cocoon fibers of the comet moth, Argema mittrei. The fibers are populated with a high density of air voids randomly distributed across the fiber cross-section but are invariant along the fiber. These filamentary air voids strongly scatter light in the solar spectrum. A single silk fiber measuring ~50 μm thick can reflect 66% of incoming solar radiation, and this, together with the fibers' high emissivity of 0.88 in the mid-infrared range, allows …


Novel Compact Narrow-Linewidth Mid-Infrared Lasers For Sensing Applications, Behsan Behzadi Jul 2018

Novel Compact Narrow-Linewidth Mid-Infrared Lasers For Sensing Applications, Behsan Behzadi

Optical Science and Engineering ETDs

The mid-infrared (2-14 μm) spectral region contains the strong absorption lines of many important molecular species, which make this region crucial for several well-know applications such as spectroscopy, chemical and biochemical sensing, security, and industrial monitoring. To fully exploit this region through absorption spectroscopic techniques, compact and low-cost narrow-linewidth (NLW) mid-infrared (MIR) laser sources are of primary importance.

This thesis is focused on three novel compact NLW MIR lasers: demonstration and characterization of a new glass-based spherical microlaser, investigation of the performance of a novel fiber laser, and the design of a monolithic laser on a silicon chip. Starting with …


Laser-Induced Recoverable Surface Patterning On Ni50ti50 Shape Memory Alloys, Saidjafarzoda Ilhom Jul 2018

Laser-Induced Recoverable Surface Patterning On Ni50ti50 Shape Memory Alloys, Saidjafarzoda Ilhom

Masters Theses & Specialist Projects

Shape memory alloys (SMAs) are a unique class of smart materials exhibiting extraordinary properties with a wide range of applications in engineering, biomedical, and aerospace technologies. In this study, an advanced, efficient, low-cost, and highly scalable laser-assisted imprinting method with low environmental impact to create thermally controllable surface patterns is reported. Two different imprinting methods were carried out mainly on Ni50Ti50 (at. %) SMAs by using a nanosecond pulsed Nd:YAG laser operating at 1064 nm wavelength and 10 Hz frequency. First, laser pulses at selected fluences were directly focused on the NiTi surface, which generated pressure pulses of up to …


Design, Fabrication, And Characterization Of A One-Dimensional Single-Material Polarizing Photonic Crystal, Ehsan Ordouie Jul 2018

Design, Fabrication, And Characterization Of A One-Dimensional Single-Material Polarizing Photonic Crystal, Ehsan Ordouie

Graduate Theses - Physics and Optical Engineering

We examine a multilayered one-dimensional (1D) polarizing photonic crystal designed and fabricated out of a single material. This polarizer is designed for high reflection of the s polarization and low reflection of p polarization at the wavelength of 632.8 nm. This device is fabricated implementing the oblique angle deposition technique to produce six-bilayers of alternating high to low indices of titanium dioxide using e-beam PVD for depositing on top of a fused silica substrate. For modeling, we used transfer-matrix method and numerical finite-difference time-domain analysis to simulate behavior of the 1D photonic bandgap structure. Both model and simulation predict better …


Broadband Measurement And Reduction Of Quantum Radiation Pressure Noise In The Audio Band, Jonathan Daniel Cripe Jun 2018

Broadband Measurement And Reduction Of Quantum Radiation Pressure Noise In The Audio Band, Jonathan Daniel Cripe

LSU Doctoral Dissertations

One hundred years after Albert Einstein predicted the existence of gravitational waves in his general theory of relativity, the Laser Interferometer Gravitational-Wave Observatory (LIGO) made the first direct detection of gravitational waves. Since the first detection of gravitational waves from a binary black hole merger, LIGO has gone on to detect gravitational waves from multiple binary black hole mergers, and more recently from a binary neutron star merger in collaboration with telescopes around the world. The detection of gravitational waves has opened a new window to the universe and has launched the era of gravitational wave astronomy.

With the first …


Super‐Resolution Imaging Of Amyloid Structures Over Extended Times By Using Transient Binding Of Single Thioflavin T Molecules, Kevin Spehar, Tianben Ding, Yuanzi Sun, Niraja Kedia, Jin Lu, George R. Nahass, Matthew D. Lew, Jan Bieschke Jun 2018

Super‐Resolution Imaging Of Amyloid Structures Over Extended Times By Using Transient Binding Of Single Thioflavin T Molecules, Kevin Spehar, Tianben Ding, Yuanzi Sun, Niraja Kedia, Jin Lu, George R. Nahass, Matthew D. Lew, Jan Bieschke

Electrical & Systems Engineering Publications and Presentations

Oligomeric amyloid structures are crucial therapeutic targets in Alzheimer's and other amyloid diseases. However, these oligomers are too small to be resolved by standard light microscopy. We have developed a simple and versatile tool to image amyloid structures by using thioflavin T without the need for covalent labeling or immunostaining. The dynamic binding of single dye molecules generates photon bursts that are used for fluorophore localization on a nanometer scale. Thus, photobleaching cannot degrade image quality, allowing for extended observation times. Super‐resolution transient amyloid binding microscopy promises to directly image native amyloid by using standard probes and record amyloid dynamics …


Imaging The Three-Dimensional Orientation And Rotational Mobility Of Fluorescent Emitters Using The Tri-Spot Point Spread Function, Oumeng Zhang, Jin Lu, Tianben Ding, Matthew D. Lew Jun 2018

Imaging The Three-Dimensional Orientation And Rotational Mobility Of Fluorescent Emitters Using The Tri-Spot Point Spread Function, Oumeng Zhang, Jin Lu, Tianben Ding, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

Fluorescence photons emitted by single molecules contain rich information regarding their rotational motions, but adapting single-molecule localization microscopy (SMLM) to measure their orientations and rotational mobilities with high precision remains a challenge. Inspired by dipole radiation patterns, we design and implement a Tri-spot point spread function (PSF) that simultaneously measures the three-dimensional orientation and the rotational mobility of dipole-like emitters across a large field of view. We show that the orientation measurements done using the Tri-spot PSF are sufficiently accurate to correct the anisotropy-based localization bias, from 30 nm to 7 nm, in SMLM. We further characterize the emission anisotropy …