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Full-Text Articles in Organic Chemistry

Developing Solvent Tolerant Microbial Membranes: Lipid Extraction And Laurdan Fluorescence Approaches For Biofuel Optimization, Gladstone Anku Aug 2026

Developing Solvent Tolerant Microbial Membranes: Lipid Extraction And Laurdan Fluorescence Approaches For Biofuel Optimization, Gladstone Anku

Electronic Theses and Dissertations

The high demand for sustainable energy has increased interest in advanced biofuels, though production is limited by solvent toxicity to microbial hosts. This study investigated the role of lipid composition in modulating membrane fluidity and solvent tolerance using Bacillus subtilis as a model organism. Five strains were cultured, and membrane lipids were extracted and analyzed using thin-layer chromatography and gas chromatography–mass spectrometry; however, inconsistent results limited compositional characterization. To address this problem, reconstituted vesicles composed of phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) were used as model membranes. Membrane fluidity was assessed using Laurdan fluorescence spectroscopy by measuring generalized polarization (GP) values …


Adsorption Of Pfas On Bridged Functionalized Organosilica Materials, Elisha Lawerh Kabutey May 2026

Adsorption Of Pfas On Bridged Functionalized Organosilica Materials, Elisha Lawerh Kabutey

Electronic Theses and Dissertations

PFAS are hazardous contaminants that have a devastating impact on human health and the environment. Their hazardous nature has led to the development of various adsorption methods for removing these contaminants from water sources. In this study, functionalized organosilica materials were synthesized from bis[3-(trimethoxysilyl)propyl] amine using the sol-gel method. The surface amino groups of the organosilica were converted into amine hydrochloride groups. Their adsorption properties were evaluated using salts of perfluorooctanoic acid, perfluorooctanesulfonic acid, and perfluorobutanesulfonic acid. Results showed excellent adsorption capacity of the materials. Adsorption of PFAS leads to particle agglomeration and flotation of the spent material. A column …


Mechanism Of Formation Of Guanine-Guanine Crosslinks As Products Of One-Electron Oxidation Of 1-Methylguanosine In Acidic Medium, Ibrahim Braimah Mr May 2026

Mechanism Of Formation Of Guanine-Guanine Crosslinks As Products Of One-Electron Oxidation Of 1-Methylguanosine In Acidic Medium, Ibrahim Braimah Mr

Electronic Theses and Dissertations

Reactive oxygen species (ROS) from normal metabolism and environmental exposure may cause oxidative DNA damage. Guanine (G) is the most oxidizable nucleobase. One-electron oxidation (OEO) of G in produces guanine radical cation (G•⁺), which deprotonates to form guanyl radical G(-H)•.This radical exists in two tautomeric forms: G(N1-H)• and G(N2-H)•. Dimerization of guanyl radical yields G-G cross-links (D1/D2); a tautomeric form for dimer formation remains elusive. This study presents the first characterization of G• dimerization products from the OEO of 1-MeGuo in acidic medium. Photolysis at 470 nm in the presence of S₂O₈²⁻ and Ru(II)(bpy)₃²⁺ generates two major stereoisomers (D1AW* and …


Unraveling Phytosulfokine Trafficking In Arabidopsis Thaliana Using Fiber-Optic Fluorescence Microscopy, Issaka Obuaba Dec 2024

Unraveling Phytosulfokine Trafficking In Arabidopsis Thaliana Using Fiber-Optic Fluorescence Microscopy, Issaka Obuaba

Electronic Theses and Dissertations

As sessile organisms, plants manage stress through complex signaling networks involving phytohormones such as phytosulfokine (PSK). PSK, a disulfated pentapeptide, regulates plant growth, development, and stress responses by interacting with specific PSK receptors (PSKRs). In this study, we explored the trafficking dynamics of PSK, its post-application fate, and the synthesis of an analog. We administered both native PSK and a fluorescent version tagged with TAMRA (5(6)-carboxytetramethylrhodamine) to various Arabidopsis thaliana genotypes, including wild type, a PSKR-deficient mutant, and a strain overexpressing PSKR1 tagged with green fluorescent protein (GFP) over the wild-type background. Fiber-optic fluorescence microscopy revealed that receptor presence influences …


Investigating Phytosulfokine Trafficking: Insights Into The Role Of Phytohormones In Plant Signaling, Martin Tindi Dec 2024

Investigating Phytosulfokine Trafficking: Insights Into The Role Of Phytohormones In Plant Signaling, Martin Tindi

Electronic Theses and Dissertations

Phytosulfokine is a critical signaling peptide involved in plant growth, development, and stress responses. This thesis investigates the trafficking mechanisms of Phytosulfokine (PSK) in Arabidopsis thaliana, employing fluorescently labelled PSK for non-destructive imaging using a custom-built fiber-optic fluorescence microscope. The study aims to determine the mobility and fate of PSK in plant tissues. Experimental results reveal the short-range and long-range movement of PSK across leaves. Additionally, the project highlights the optimization of non-destructive imaging techniques and the synthesis of caged dexamethasone to facilitate optogenetic studies. These findings provide new insights into PSK trafficking mechanisms in plants, enhancing our understanding …


Application Of Functionalized Organosilicas In Adsorption Of Nitrates, Stephen Amoako Aug 2024

Application Of Functionalized Organosilicas In Adsorption Of Nitrates, Stephen Amoako

Electronic Theses and Dissertations

This study addresses the critical environmental issue of elevated nitrate levels in water bodies, primarily due to excessive use of nitrogenous fertilizers and improper waste disposal. It focuses on reducing nitrate concentrations in polluted water to permissible levels through the effectiveness of hybrid materials in nitrate adsorption. We synthesized nine amino-functionalized adsorbents using grafting and sol-gel techniques. Batch adsorption tests confirmed the high nitrate adsorption capacities of these adsorbents, with sol-gel materials showing the highest efficiency due to their abundant amino group contents. Among these, the surfactant-free, sol-gel adsorbent was the most effective, combining ease of synthesis with cost-efficiency. Our …


Synthesis Of Bacterial Glycerophospholipids For Biomembrane Model Studies: A Means To Advanced Biofuels, Felix Adulley Dec 2023

Synthesis Of Bacterial Glycerophospholipids For Biomembrane Model Studies: A Means To Advanced Biofuels, Felix Adulley

Electronic Theses and Dissertations

To reduce reliance on fossil fuels, sustainable biofuels are being pursued, especially advanced biofuels like 1-butanol that have higher energy content and greater compatibility with existing infrastructure than ethanol. A persistent challenge is the yield-limiting toxicity of biofuels and process solvents, such as tetrahydrofuran, to the microbes that ferment biomass into biofuel. The cell membrane is a focal point of toxicity, and understanding how it interacts with fuels and solvents is key to improving yield. Phospholipid bilayers are the core of biomembranes, and model biomembranes of defined composition provide the ideal platform for biophysical studies. To this end, glycerophospholipids characteristic …


Extraction, Purification, And Characterization Of Potential Bioactive Compounds Produced By Janthinobacterium Lividum Tajx1901, Andy Elorm Agbakpo Aug 2023

Extraction, Purification, And Characterization Of Potential Bioactive Compounds Produced By Janthinobacterium Lividum Tajx1901, Andy Elorm Agbakpo

Electronic Theses and Dissertations

Underexplored environments such as soil samples continue to be an untapped source of bacterial strains with great potential to produce secondary metabolites for medicinal applications. As a result, these microorganisms represent a broad and yet unknown reservoir of new strains capable of producing these novel compounds. The current research primarily seeks to perform the isolation, purification, and characterization of secondary metabolites from a soil bacterium (Janthinobacterium lividum TAJX1901). The isolated soil bacterium was successfully cultured on rich media agar plates, followed by extraction using methanol and chloroform. The purification methods utilized include flash column chromatography, preparative thin-layer chromatography, and …


Mechanisms Of Formation Of Novel Guanine-Guanine Cross-Links As Major End Products During One-Electron Oxidation Of Guanine Derivatives, Evan Dunn May 2023

Mechanisms Of Formation Of Novel Guanine-Guanine Cross-Links As Major End Products During One-Electron Oxidation Of Guanine Derivatives, Evan Dunn

Undergraduate Honors Theses

Guanine (G), as the most oxidizable base in DNA, is the major focus of studies of oxidation damage to DNA. The present thesis reports the first detailed characterization of G dimerization products potentially resulting from recombination of neutral G• radicals. Previous research has discovered a novel type of products of G• dimerization, D1 and D2, formed from one-electron oxidation of G derivatives. However, the mechanism of this dimerization remains elusive. While there appears to be a tautomeric equilibrium between two forms of G•, G(N1-H)• or G(N2-H)•, it remains unclear which intermediate participates in the formation of …


Synthesis Of A Phenyl Substituted Zinc Dipyrrin Complex For The Purpose Of Analyzing Aromatic Substitutions On The Characteristics Of Compounds Of This Class, Kole Owen May 2023

Synthesis Of A Phenyl Substituted Zinc Dipyrrin Complex For The Purpose Of Analyzing Aromatic Substitutions On The Characteristics Of Compounds Of This Class, Kole Owen

Undergraduate Honors Theses

The field of photochemistry is as innovative in development as it is broad in application. However, utilization of energy from the sun’s electromagnetic radiation remains secondary to the combustion of fossil fuels for the global energy consumption. This is neither a sustainable nor renewable system, and it has contributed to a major decline in the health of our global environment as the greenhouse gases emission has led to an incline in global temperatures and ocean acidity. To develop effective ways to utilize solar energy, experimental effort is being directed towards the understanding of photosensitizers, molecules which absorb solar radiation and …


Non-Destructive Imaging Of Phytosulfokine Trafficking In Plants Using Fiber-Optic Fluorescence Microscopy, Bernard Abakah May 2023

Non-Destructive Imaging Of Phytosulfokine Trafficking In Plants Using Fiber-Optic Fluorescence Microscopy, Bernard Abakah

Electronic Theses and Dissertations

Plants secrete peptide ligands and use receptor signaling to respond to stress and control development. Understanding these phenomena is key to improving plant health and productivity for food, fiber, and energy applications. Phytosulfokine (PSK), a sulfated peptide hormone, regulates plant cell division, growth, and stress tolerance via specific phytosulfokine receptors (PSKRs). This study uses fiber-optic fluorescence microscopy to elucidate trafficking of PSK in live plants. The microscope features two-color optics and an objective lens connected to a 1-m coherent imaging fiber mounted on either a conventional upright microscope body or 5-axis positioning system (X–Y–Z plus pitch and yaw). PSK and …


Folic Acid – Carbon Dots – Doxorubicin Nanoparticles As Cancer Theranostic, Michael Tetteh Dec 2022

Folic Acid – Carbon Dots – Doxorubicin Nanoparticles As Cancer Theranostic, Michael Tetteh

Electronic Theses and Dissertations

This work focused on engineering bi-functionalized nanoparticles (NPs) based on carbon dots (CDs) to improve early cancer detection and treatment. Therefore, using folic acid (FA) as a targeting agent, the CDs were prepared to deliver high concentrations (HC) of doxorubicin (DOX) and gemcitabine (GEM) covalently and non-covalently to cancer cells. The prepared FA-CDs-DOX/GEM-HC NPs were characterized using ultraviolet-visible spectroscopy, fluorescence spectroscopy, and Fourier transform infrared spectroscopy. Assessment of the drug loading capacity (DLC) and drug loading efficiency (DLE) indicated that the non-covalent NPs have low DLC but high DLE compared to the relatively low DLE and high DLC of covalent …


Friedel-Crafts Alkylation Of Benzene With A Superacid Catalyst, Josh T. Cutright May 2022

Friedel-Crafts Alkylation Of Benzene With A Superacid Catalyst, Josh T. Cutright

Undergraduate Honors Theses

Long-chain alkylbenzenes are industrially synthesized precursors to commercial surfactants such as laundry detergent. The currently used catalysts in the processes of their synthesis are corrosive and harmful to the environment. These problems can be avoided utilizing heterogeneous highly acidic catalysts. Solid catalysts do not corrode equipment and are relatively simple to remove from the post-reaction mixture. Phosphotungstic acid (PTA) supported on silica gel could be a possible catalyst due to its high acidity with an estimated pKa ≈ -13. The catalyst PTA-SiO2 was prepared via the sol-gel method to covalently embed it in a silica support. The catalyst was …


Study Of The Cell Membrane And The Synthesis Of Chimeric Human Bacterial Phospholipids, Opeyemi O. Tade Dec 2021

Study Of The Cell Membrane And The Synthesis Of Chimeric Human Bacterial Phospholipids, Opeyemi O. Tade

Electronic Theses and Dissertations

Phospholipid bilayers are the principal component of the cell membrane. Membranes ensure the maintenance of processes required for cells’ survival by regulating the inflow and outflow of nutrients and other molecules using membrane proteins. However, studying the cell membrane is challenging because of its complexity and small size. In-vitro membrane models made of phospholipids are important tools for studying membranes. In this work, we aim to study the fluidity of phospholipid bilayers of different lipids using general polarization (GP) of the fluorescent probe Laurdan as a measure. We will focus on the relative importance of head groups and fatty acids …


Preparation And Study Of Bacterial Membrane Models, Enoch Asimbisa Dec 2021

Preparation And Study Of Bacterial Membrane Models, Enoch Asimbisa

Electronic Theses and Dissertations

Fuel molecules are organic solvents that have disruptive effects on the bacterial membrane. This is a significant barrier in biofuel production, as it limits the fuel concentration that can be achieved through fermentation. One potential way of overcoming this barrier is to identify lipid compositions that can better withstand solvent stress, for which it is important to understand how organic solvents disrupt the membrane. Use of biophysical characterization techniques to quantify physical properties like fluidity and thickness will enable us to understand the mechanism by which solvents disrupt membranes. Native membranes are very complex, and we sought to develop in-vitro …


Synthesis Of Phosphatidylethanolamine Lipids For Model Studies Of The Cell Membrane, John Hayford G. Teye-Kau Dec 2021

Synthesis Of Phosphatidylethanolamine Lipids For Model Studies Of The Cell Membrane, John Hayford G. Teye-Kau

Electronic Theses and Dissertations

Concerns about global warming have resulted in a surge of research into alternatives to fossil fuels. In recent years, biofuels have gained traction due to their low environmental impact. Biofuel production most commonly employs microorganisms to convert biomass to fuel for industrial and transportation applications. Compounds made in biofuel production, however, are toxic to cell membranes and disrupt their integrity, harming the microorganisms and limiting biofuel yield. A key to overcoming this challenge is understanding how fuels interact with microorganisms’ cell membranes, which perform a host of functions, including transport, cell recognition, transduction, and movement. Phospholipids are the cell membrane’s …


Synthesis Of Phytosulfokine Analogs As Probes For Studying Plant Signaling And Molecular Trafficking, Thomas Ntim Dec 2021

Synthesis Of Phytosulfokine Analogs As Probes For Studying Plant Signaling And Molecular Trafficking, Thomas Ntim

Electronic Theses and Dissertations

Plants are exposed to a wide range of biotic and abiotic stresses that hinder their growth and reduce crop productivity. In their adaptive response, plants use signaling molecules that are trafficked throughout the plant. This research focuses on the chemical synthesis and assessment of analogs of the plant signal phytosulfokine (PSK, a sulfated pentapeptide), its delivery to plants and its observation using a fiber-optic fluorescence microscope. PSK regulates growth, cell expansion, heat tolerance, and tissue longevity. Analogs of PSK were synthesized using solid-phase peptide synthesis. Pure PSK and TAMRA-labeled PSK were delivered into the wild-type Arabidopsis thaliana Col-0 and a …


Synthesis Of Flagellin 22 As A Probe For Plant Signaling And Molecular Trafficking Towards Improved Crops, Edward Offei Aug 2021

Synthesis Of Flagellin 22 As A Probe For Plant Signaling And Molecular Trafficking Towards Improved Crops, Edward Offei

Electronic Theses and Dissertations

Plant signaling involves the transport of information within and between plant cells from receptors to effectors. Plants are affected by biotic and abiotic stress conditions like insect attack and extreme temperatures, respectively, which cause disease, the induction of senescence and the reduction of crop yield. To improve plant traits for feed, fiber, and energy applications, it is critical to understand the short- and long-range signaling mechanisms plants use to control growth, biomass composition, senescence and responses to environmental stresses. It is known that many plant signaling molecules have profound effects on plants, through mechanisms that remain largely obscure. A key …


Synthesis Of The Diazonium Zwitterionic Polymer/Monomer For Use As The Electrolyte In Polymer Electrolyte Membrane (Pem) Fuel Cells, Josiah Marshall Aug 2021

Synthesis Of The Diazonium Zwitterionic Polymer/Monomer For Use As The Electrolyte In Polymer Electrolyte Membrane (Pem) Fuel Cells, Josiah Marshall

Electronic Theses and Dissertations

My research goals are to synthesize new zwitterionic perfluorosulfonimide (PFSI) monomer/polymers. They are expected to replace traditionally used perfluorosulfonic acid (PFSA) polymers as the electrolyte in PEM fuel cells. For the PFSI monomer preparation, we designed a nine-step synthesis route. Thus far, I have successfully completed the synthesis of 4- (2-bromotetrafluoroethoxy)-benzenesulfonyl amide, 4-acetoxybenzenesulfonic acid sodium salt, and 4-chlorosulfonyl phenyl acetate. The coupling reaction of 4-(2-bromotetrafluoroethoxy)- benzenesulfonyl amide with 4-chlorosulfonyl phenyl acetate, was troublesome due to slow reaction kinetics and byproducts. Additionally, I did a methodology study for the homopolymerziation of the perfluoro 3(oxapent-4-ene) sulfonyl fluoride monomer. We compared the weight …


Synthesis Of A Zinc Dipyrrin Complex For Photocatalytic Reduction Of Co2, Sylvia Meredith May 2021

Synthesis Of A Zinc Dipyrrin Complex For Photocatalytic Reduction Of Co2, Sylvia Meredith

Undergraduate Honors Theses

Zinc dipyrrin complexes have the potential to act as cheap, effective photosensitizers. Synthesizing and studying different types could lead to more efficient solar energy harvesting processes, especially the production of solar fuel. Here, two attempts to synthesize 1,3,7,9-tetraphenyl-5-mesityldipyrromethene are reported and discussed. According to 1H NMR, the first synthesis attempt was not successful. The second synthesis attempt was not purified effectively, so 1H NMR produced inconclusive results. Further purification strategies or alternate synthesis methods are required.


Theranostic Nanoparticles Folic Acid-Carbon Dots-Drug(S) For Cancer, Godwin Kweku Babanyinah May 2021

Theranostic Nanoparticles Folic Acid-Carbon Dots-Drug(S) For Cancer, Godwin Kweku Babanyinah

Electronic Theses and Dissertations

This study aims to prepare theranostic nanoparticles (NPs) that are expected to increase cancer diagnostics and therapeutic efficacy. We prepared the NPs constituting carbon dots (CDs) as an imaging agent, folic acid as a targeting agent, doxorubicin (DOX), or gemcitabine (GEM) as chemotherapy agents. The NPs include noncovalent FA-CDs-DOX, covalent CDs-FA-DOX, and covalent FA-CDs-GEM. Through ultraviolet-visible spectroscopy, fluorescence spectroscopy, and Fourier transform-infrared spectroscopy, the fabrication of these NPs was confirmed. It was discovered that the high drug loading efficiency is the noncovalent series while the high drug loading capacity is the covalent series The in-vitro pH-dependent drug release data indicate …


Alkylation Of Benzene On Immobilized Phosphotungstic Acid, Kofi Kankam Dec 2020

Alkylation Of Benzene On Immobilized Phosphotungstic Acid, Kofi Kankam

Electronic Theses and Dissertations

Linear alkylbenzenes (LAB) are key intermediates in the synthesis of linear alkylbenzene sulfonate surfactants that are used in the manufacture of detergents. Production of LAB with traditional Lewis acids as catalysts, such as hydrofluoric acid, results in the formation of large amounts of toxic wastes and corrosion of industrial equipment. Phosphotungstic acid (PTA) has gained much attention in recent years as a solid catalyst for various alkylation reactions. This research work aims to develop a novel material based on PTA-containing silica gel, which can effectively catalyze LAB synthesis. Sol-gel synthesis of silica gel in the presence of PTA and tetraethyl …


Mesoporous Adsorbents For Perfluorinated Compounds, Bertha Lotsi May 2020

Mesoporous Adsorbents For Perfluorinated Compounds, Bertha Lotsi

Electronic Theses and Dissertations

Effective adsorbents for polyfluorinated compounds (PFCs) were successfully prepared. And they were tested in the adsorption of perfluorooctanoic and perfluorooctanesulfonic acids. Bridged silsesquioxanes containing secondary and tertiary amino groups were synthesized by sol-gel condensation of bis[3-(trimethoxysilyl)propyl]amine and bis[3-(methylamino)propyl]-trimethoxysilane in acidic media with surfactants. Obtained materials are mesoporous with a high BET surface area. They combine high structural stability with a high concentration of surface amino groups serving as adsorption sites. Batch adsorption tests demonstrated their extremely high adsorption capacity on PFCs: in some experiments, it reached up to 88% of the adsorbent weight. Adsorption of PFCs changed the surfaces of …


Immobilization Of Phosphotungstic Acid On Silica Surface For Catalytic Alkylation Of Aromatic Compounds, Anastasia Kuvayskaya May 2020

Immobilization Of Phosphotungstic Acid On Silica Surface For Catalytic Alkylation Of Aromatic Compounds, Anastasia Kuvayskaya

Electronic Theses and Dissertations

Superacidic mesoporous materials containing covalently embedded PTA were synthesized by sol-gel method. Tetraethyl orthosilicate (TEOS) and phosphotungstic acid (PTA) were used as precursors in the synthesis, ionic and nonionic surfactants were used as pore-forming agents, the reaction proceeded in acidic media. TEM images revealed mesoporous structure with embedded PTA clusters. FT-IR spectra of obtained materials contained characteristic bands of PTA at 957 cm-1. Synthesized catalysts had high BET surface area and high concentration of acidic sites. Alkylation of 1,3,5-trimethylbenzene by 1-decene demonstrated high catalytic activity. The catalyst obtained with Pluronic P123 as a template was the most effective and resulted …


Synthesis And Study Of Hydroxytyrosol Derivatives, Ebenezer Ametsetor Dec 2019

Synthesis And Study Of Hydroxytyrosol Derivatives, Ebenezer Ametsetor

Electronic Theses and Dissertations

Hydroxytyrosol is one of the most powerful known antioxidants. It is a naturally occurring polyphenol, most commonly produced in olive trees, (Olea europaea). The remarkable antioxidant and pharmacological properties of hydroxytyrosol has made it an outstanding compound in the polyphenol family and of great interest to many researchers. Hydroxytyrosol can scavenge free radicals produced during cellular oxidative stress and helps to protect the integrity of cells in living systems. Despite its numerous biological and pharmacological uses, it is found in very low concentration in olive oil, this limits its availability for biomedical applications. This work reports a novel and effective …


Structure Elucidation Of A Pyrrolobenzodiazepine Alkaloid And A Biologically Active Polyketide Produced By Rhodococcus Sp. Mtm3w5.2 Via Two-Dimensional Nmr Spectroscopy, Garrett Johnson Dec 2019

Structure Elucidation Of A Pyrrolobenzodiazepine Alkaloid And A Biologically Active Polyketide Produced By Rhodococcus Sp. Mtm3w5.2 Via Two-Dimensional Nmr Spectroscopy, Garrett Johnson

Electronic Theses and Dissertations

As the battle against ever-increasing drug resistence bacteria rages on, novel and sometimes more complex natural products can be used to combat this. In this study, two-dimensional NMR techniques were utilized to collect a complete spectral data set for two natural products. The first structure, a synthesized Pyrrolobenzodiazepine alkaloid natural product was confirmed through these methods. The second, a strain of Rhodococcus, MTM3W5.2, produces a novel antibacterial molecule in broth cultures and the active compound was fractionated using a Sephedex LH-20 column. Chromatographic purification yielded a pure sample at 58.90 minutes, RT.58. HRMS data deduced an exact mass of …


Preparation Of Folic Acid-Carbon Dots-Doxorubicin Nanoparticles As Targeting Tumor Theranostics, Samson Dada Dec 2019

Preparation Of Folic Acid-Carbon Dots-Doxorubicin Nanoparticles As Targeting Tumor Theranostics, Samson Dada

Electronic Theses and Dissertations

Carbon dots (CDs) have attracted much attention as an excellent gene/drug delivery and biological imaging agent for early cancer theranostics. In this study, we prepared two series of nanoparticles (NPs), which are composed of (CDs) with a targeting agent, folic acid (FA), and a chemotherapeutic agent Doxorubicin (Dox). All the NPs and their intermediates were characterized using ultraviolet-visible spectroscopy (UV-vis), fluorescence spectroscopy, and Fourier transform-infrared spectroscopy (FT-IR). The drug loading capacity (DLC) and drug loading efficiency (DLE) of two series of FA-CDs-Dox were assessed using UV-vis absorption spectroscopy at the wavelength of 485 nm. Both showed good DLE and DLC …


Synthesis, Characterization And Biological Evaluation Of Novel (S,E)-11-[2-(Arylmethylene) Hydrazono] Pyrrolo [2,1-C] [1,4] Benzodiazepine Derivatives, David Mingle Aug 2019

Synthesis, Characterization And Biological Evaluation Of Novel (S,E)-11-[2-(Arylmethylene) Hydrazono] Pyrrolo [2,1-C] [1,4] Benzodiazepine Derivatives, David Mingle

Electronic Theses and Dissertations

Pyrrolo [2,1-c] [1,4] benzodiazepine (PBD) is a class of natural products obtained from various actinomycetes which have both anti-tumor and antibiotic activities and can bind to specific sequences of DNA. PBD-dilactam was initially produced using isatoic anhydride and (L)-proline which was then converted to the PBD-thiolactam using Lawesson's reagent. Reaction of thiolactam with hydrazine in ethanol afforded PBD-11-hydrazinyl. Condensation of 11-hydrazinyl PBD with aldehydes possessing various substitutions was performed to obtain (S,E)-11-[2-(arylmethylene) hydrazono] pyrrolo [2,1-c] [1,4] benzodiazepine derivatives. 1HNMR, 13C-NMR, DEPT, IR, GC-MS and X-ray crystallography were used for the characterization. Inhibition activity of the products …


Synthesis Of Diazonium N-(Perfluoroalkyl) Benzenesulfonimide Polymers For Proton Exchange Membrane Fuel Cells (Pemfcs), Helal Alharbi Aug 2019

Synthesis Of Diazonium N-(Perfluoroalkyl) Benzenesulfonimide Polymers For Proton Exchange Membrane Fuel Cells (Pemfcs), Helal Alharbi

Electronic Theses and Dissertations

The objective of the research is to synthesize the diazonium N-(perfluoroalkyl) benzenesulfonimide (PFSI)zwitterionicpolymers as electrolytes in polymerelectrolyte membrane (PEM) fuel cells. The proposed diazoniumPFSI zwitterionic polymer (I) is expected to enhance the thermal and chemical stability, increase the proton conductivity of electrolytes, and improve the catalyst efficiency for PEM fuel cells. Synthesis of the perfluorobenzoyl peroxide initiator, homopolymerization of perfluoro (3-oxapent-4-ene) sulfonyl fluoride,coupling reaction with4-sulfamonylacetanilide, couplingreaction with 4-nitrobenzene sulfonyl amide, n-deacetylation reaction, and diazotization reactionhave been carried outsuccessfully in the lab. The intermediate chemicals are characterized by GC-MS, IR, NMR, and GPC spectroscopies.


Synthesis And Evaluation Of 1,2,4-Oxadiazolidinones: The Search For A Potential Non-Β-Lactam Β-Lactamase Inhibitors., Chimdi Eke Kalu May 2019

Synthesis And Evaluation Of 1,2,4-Oxadiazolidinones: The Search For A Potential Non-Β-Lactam Β-Lactamase Inhibitors., Chimdi Eke Kalu

Electronic Theses and Dissertations

β-lactam antibiotics have been the most widely used drug of choice to combat infectious disease caused by bacteria. Unfortunately, their effectiveness is drastically threatened by bacterial β-lactamases. β-lactamases is responsible for the resistance to most antibiotic drugs. For decades, β-lactam β-lactamases inhibitors have been used to reduce bacterial resistance; however, in this study 1,2,4-oxadiazolidinone derivatives as a non-β-lactam β-lactamases inhibitor against TEM-1 and P99 β-lactamases. The significance of oxadiazolidinone is the prominent five-membered ring scaffold in its structure, which is configurationally stable and present in other biologically active compounds such as linezolid and avibactam. Oxadiazolidinones were synthesized by treating nitrones …