Physical Sciences and Mathematics Commons^™

Monitoring Doxorubicin Cellular Uptake And Trafficking Using In Vitro Raman Microspectroscopy: Short And Long Time Exposure Effects On Lung Cancer Cell Lines, Zeineb Farhane, Franck Bonnier, Hugh Byrne

Articles

Raman microspectroscopy is a non-invasive, in vitro analytical tool which is being increasingly explored for its potential in clinical applications and monitoring the uptake, mechanism of action and cellular interaction at a molecular level of chemotherapeutic drugs, ultimately as a potential label-free preclinical screening and companion diagnostic tool. In this study, doxorubicin (DOX), a “gold standard” chemotherapeutic drug, is employed as a model in the in vitro lung cancer cell line A549 in order to demonstrate the potential of Raman microspectroscopy to screen and identify spectroscopic markers of its trafficking and mechanism of action. Confocal laser scanning microscopy (CLSM) was …

Determination Of Spectral Markers Of Cytotoxicity And Genotoxicity Using In Vitro Raman Microspectroscopy: Cellular Responses To Polyamidoamine Dendrimer Exposure, Esen Efeoglu, Alan Casey, Hugh Byrne

Articles

Although consumer exposure to nanomaterials is ever increasing, with potential increased applications in areas such as drug and/or gene delivery, contrast agents and diagnosis, determination of cyto- and geno- toxic effect of nanomaterials on human health and the environment still remains challenging. Although many techniques have been established and adapted to determine the cytotoxicity and genotoxicity of nano-sized materials, these techniques remain limited by the number of assays required, total cost, use of labels and they struggle to explain the underlying interaction mechanisms. In this study, Raman microspectroscopy is employed as an in vitro label free high content screening technique …

A Natural, Calcium-Rich Marine Multi-Material Complex Preserves Bone Structure, Composition And Strength In An Ovariectomized Rat Model Of Osteoporosis, Orlaith Brennan, Joseph Sweeney, Brian O'Meara, Amro Widaa, Franck Bonnier, Hugh Byrne, Denis O'Gorman, Fergal O'Brien

Articles

Calcium supplements are used as an aid in the prevention of osteopenia and osteoporosis and also for the treatment of patients when used along with medication. Many of these supplements are calcium carbonate based. This study compared a calcium-rich, marine multi-mineral complex (Aquamin) to calcium carbonate in an ovariectomised rat model of osteoporosis in order to assess Aquamin’s efficacy in preventing the onset of bone loss. Animals were randomly assigned to either non-ovariectomy control (Control), ovariectomy (OVX) plus calcium carbonate, ovariectomy plus Aquamin or ovariectomy plus Aquamin delay where Aquamin treatment started 8 weeks post OVX. At the end of …

Advancing Vibrational Spectroscopy For Cellular And Sub Cellular Analysis: Raman Spectroscopy As An In Vitro Chemotherapeutic Screeening And Assessment Protocol, Zeineb Farhane

Doctoral

Vibrational spectroscopy, including Raman micro-spectroscopy, has attracted considerable attention over the last few years, as a powerful, non-invasive tool for clinical applications, especially in cancer diagnosis, in vivo and ex vivo. As a molecular fingerprinting technique with optical resolution, Raman micro-spectroscopy is able to monitor biochemical processes, drug uptake, efficacy and mode of action and mechanisms of interaction of chemotherapeutic drugs at a subcellular level. In vitro applications may be more strategically achievable, and can help guide drug design and discovery, and eventually evaluate To this end, different lung cell lines were used and Raman micro-spectroscopy was coupled to valuable …

Advancing Vibrational Spectroscopy For Cellular And Sub Cellular Analysis: Raman Spectroscopy As A Novel In Vitro Nanotoxicological Assessment Protocol, Esen Efeoglu

Doctoral

This work is designed to establish a ‘High content Nanotoxicological Screening method’ using in vitro Raman microspectroscopy. The undeniable increase of nanotechnology based products has brought challenges in terms of determining their toxicological properties. Considering the total time and cost of screening nanomaterials by conventional methods, the need for a rapid, label-free technique which will provide a wide range of information on multiple parameters is unquestionable. This study investigated the applicability of Raman microspectroscopy as a High Content Screening technique to clarify cell-nanoparticle interaction by determining the localisation of the nanoparticles and consequent effects in these localised areas in terms …

Freedericksz Transition In Nematic Liquid Crystal Couette Flow, Arup Mukherjee

Department of Mathematics Faculty Scholarship and Creative Works

This article investigates the effects of an external magnetic field on the Freedericksz transition for an elastically anisotropic nematic liquid crystal sample occupying the annular region between two concentric cylinders in relative (slow) rotation. Assuming both azimuthal and radial magnetic fields and strong anchoring conditions for the liquid crystal director perpendicular to the surface of the cylinders, we investigate and characterize the differences in the director distortions and the critical field value for the onset of the transition.

Development Of The Framework For A Lean, Energy Efficient, And Environmentally Friendly Port: Umm Qasr Port As A Case Study, Safaa A.J. Alfayyadh

World Maritime University Ph.D. Dissertations

The research focus is to examine rigorously how the implementation of Lean within the Umm Qasr Port improves the operation processes and to explore the Lean impact on environment improvement and energy efficiency management. In this research, the ROPMEE model has been developed by the researcher to evaluate the service quality in the cargo delivery process in the Port of Umm Qasr as it covers all the functional and non-functional areas in the cargo delivery process compared to other quality dimensions. The findings confirm that the process quality dimension is the most influential factor in service quality in the Port …

A Transfer Matrix Approach To Aid In The Design And Optimization Of Hybrid Advanced Passive Structures For Enhancing Photovoltaic Efficiency, James Walshe, Sarah Mccormack, Hind Ahmed, John Doran

Conference Papers

The addition of a luminescent down-shifting (LDS) layer directly onto a photovoltaic (PV) cell introduces additional loss mechanisms within the system. The combination of non-ideal photo-luminescent materials encapsulated within a limited range of viable host materials, with the increased reflection losses arising from the newly created interface represent losses which must be overcome for LDS to offer an enhancement to the underlying cells efficiency. Exploiting the interaction between the highly enhanced electric fields established close to a metal nanoparticles (MNP’s) surface is one route aimed at mitigating the poor optical properties of the luminophore-host combinations available. Alternative approaches, aimed at …

Proactive It Incident Prevention: Using Data Analytics To Reduce Service Interruptions, Mark G. Malley

Walden Dissertations and Doctoral Studies

The cost of resolving user requests for IT assistance rises annually. Researchers have demonstrated that data warehouse analytic techniques can improve service, but they have not established the benefit of using global organizational data to reduce reported IT incidents. The purpose of this quantitative, quasi-experimental study was to examine the extent to which IT staff use of organizational knowledge generated from data warehouse analytical measures reduces the number of IT incidents over a 30-day period, as reported by global users of IT within an international pharmaceutical company headquartered in Germany. Organizational learning theory was used to approach the theorized relationship …

E-Business Strategy To Adopt Electronic Banking Services In Ethiopia, Teklebrhan Woldearegay Gebreslassie

Walden Dissertations and Doctoral Studies

E-banking services in Ethiopia are increasing among low-income populations; however, with over 53 million mobile service users countrywide, more than 85% of the population still lacks access to banking services. A single case study was used to explore e-business strategies that bank managers use to promote the adoption of electronic banking services to the unbanked population in Ethiopia. The extended resource-based view of strategy served as the conceptual framework for this study. Data were collected from interviews with 12 experienced bank managers from leading commercial bank in Ethiopia. Data were analyzed using coding techniques and word clustering, with the help …

How Attitude Toward The Behavior, Subjective Norm, And Perceived Behavioral Control Affects Information Security Behavior Intention, David Philip Johnson

Walden Dissertations and Doctoral Studies

The education sector is at high risk for information security (InfoSec) breaches and in need of improved security practices. Achieving data protections cannot be through technical means alone. Addressing the human behavior factor is required. Security education, training, and awareness (SETA) programs are an effective method of addressing human InfoSec behavior. Applying sociobehavioral theories to InfoSec research provides information to aid IT security program managers in developing improved SETA programs. The purpose of this correlational study was to examine through the theoretical lens of the theory of planned behavior (TPB) how attitude toward the behavior (ATT), subjective norm (SN), and …

Strategies To Support Survivors Of Corporate Downsizing, Joseph Linton

Walden Dissertations and Doctoral Studies

Between 2003 and 2011, 1.2 million U.S. workers were laid off every year. Some organizational leaders of information technology/telecommunications organizations lack strategies to improve affective commitment to the organization after downsizing events. The strains of overwork, organizational changes, anxiety over job insecurity, and lack of choice often result in physical illness, emotional trauma, and extreme disengagement termed survivor syndrome. The purpose of this single case study was to explore strategies organizational leaders in the Midwest United States in information technology/telecommunications organizations used to improve affective commitment to the organization after downsizing. The conceptual framework was Herzberg's 2-factor theory. Data were …

Relationship Between Corporate Governance And Information Security Governance Effectiveness In United States Corporations, Robert Elliot Davis

Walden Dissertations and Doctoral Studies

Cyber attackers targeting large corporations achieved a high perimeter penetration success rate during 2013, resulting in many corporations incurring financial losses. Corporate information technology leaders have a fiduciary responsibility to implement information security domain processes that effectually address the challenges for preventing and deterring information security breaches. Grounded in corporate governance theory, the purpose of this correlational study was to examine the relationship between strategic alignment, resource management, risk management, value delivery, performance measurement implementations, and information security governance (ISG) effectiveness in United States-based corporations. Surveys were used to collect data from 95 strategic and tactical leaders of the 500 …

On Comparative Algorithmic Pathfinding In Complex Networks For Resource-Constrained Software Agents, Michael Moran

Walden Dissertations and Doctoral Studies

Software engineering projects that utilize inappropriate pathfinding algorithms carry a

significant risk of poor runtime performance for customers. Using social network theory,

this experimental study examined the impact of algorithms, frameworks, and map

complexity on elapsed time and computer memory consumption. The 1,800 2D map

samples utilized were computer random generated and data were collected and processed

using Python language scripts. Memory consumption and elapsed time results for each of

the 12 experimental treatment groups were compared using factorial MANOVA to

determine the impact of the 3 independent variables on elapsed time and computer

memory consumption. The MANOVA indicated a …

Strategies To Minimize The Effects Of Information Security Threats On Business Performance, Stella Ifeyinwa Okoye

Walden Dissertations and Doctoral Studies

Business leaders in Nigeria are concerned about the high rates of business failure and economic loss from security incidents and may not understand strategies for reducing the effects of information security threats on business performance. Guided by general systems theory and transformational leadership theory, the focus of this exploratory multiple case study was to explore the strategies small and medium-sized enterprise (SME) leaders use to minimize the effects of information security threats on business performance. Semistructured interviews were conducted with 5 SME leaders who worked in SME firms that support oil and gas industry sector in Port Harcourt, Nigeria, had …

Nursing Informatics Competency Program, Kristina Ann Dunn

Walden Dissertations and Doctoral Studies

Currently, C Hospital lacks a standardized nursing informatics competency program to validate nurses' skills and knowledge in using electronic medical records (EMRs). At the study locale, the organization is about to embark on the implementation of a new, more comprehensive EMR system. All departments will be required to use the new EMR, unlike the current policy that allows some areas to still document on paper. The Institute of Medicine, National League of Nursing, and American Association of Colleges of Nursing support and recommend that information technology be an essential core competency for nurses. Evidence of the need for nursing informatic …

Urban Dwellers Experiences Regarding Loss Of Natural Environments Due To Rapid Urbanization, Erica Montanye

Walden Dissertations and Doctoral Studies

Little is known about how residents of rapidly growing cities are impacted by the loss of natural environments. Large cities are expanding at an exponential rate, reducing the presence of, and access to, natural environments for urban dwellers. Many benefits to human health regarding the presence of natural environments near where people live and work are known, but impacts of the loss of natural environments for urban dwellers are unknown. The purpose of this qualitative investigation was to understand residents' experiences regarding the loss of natural environments and related impacts. Attention restoration theory and place attachment were the theoretical lenses …

Constraining Times Of Extension In The Southern Rio Grande Rift And Basin And Range Using Apatite And Zircon (U-Th)/He Thermochronology, Julian Biddle

Open Access Theses & Dissertations

The Rio Grande rift and the Basin and Range are adjacent extensional domains that have evolved contemporaneously in western North America. Although the rift is often considered to be the easternmost boundary of Basin and Range extension, there is geologic and geophysical evidence that suggests the two are discrete provinces. Existing low-temperature thermochronologic data indicate that a period of synchronous extension occurred across the entire length of the rift from ~25-10 Ma. However, these studies were more focused on the northern and central sections of the rift, and thermochronologic data remains sparse in southern New Mexico. We collected rock samples …

A Study Of Hippocampal Microglia Distribution In Pre-Adolescent Mice Chronically Exposed To Lead, Salvador Dominguez

Open Access Theses & Dissertations

Background: Lead is a major unresolved child health hazard. Children are uniquely vulnerable to the effects of lead exposure, which include disruption of brain development and brain function. How lead exposure alters brain development and brain function is not currently known. Our laboratory has been developing a mouse model to understand effects of low-level lead exposure in the developing brain. In previous studies, we have shown that early chronic low level lead exposure diminished the number of microglia in the dentate gyrus region of the hippocampus, an area responsible for learning and memory. Building on the results of the previous …

Structural Modifications In The Rbxcs1-Xpo 4 (0 ≤ X ≤ 1) Superprotonic Conductor Series: A Single-Crystal X-Ray Diffraction And Impedance Spectroscopy Study, Andres Jose Encerrado Manriquez

Open Access Theses & Dissertations

We have used single-crystal X-ray diffraction measurements to investigate the structural modifications induced by Rb-doping of the protonic conductor CsH2PO4. Data collected on the RbxCs 1-xH2PO4 (0 ≤ x ≤ 1) series shows that the monoclinic P21/m CsH2PO4 presentation persists upon Rb-doping up until x = 0.8. Rb0.8Cs0.2H2PO 4 exhibits a previously unreported P21/c monoclinic structure, where the mirror plane is lost and disorder is present in the PO4 tetrahedra even at room temperature. Higher levels of x display a tetragonal I-42d unit cell isomorphic with the known structure of RbH2PO 4. The temperature dependence of the proton conductivity determined …

Spectroscopic And Microscopic Analysis Of Graphene For Sensor Applications, Tamanna Tasneem Khan

Open Access Theses & Dissertations

As a two-dimensional material, graphene shows very good thermal and electrical conductivities, which, with its unique optical properties, makes it suitable for a variety of applications. In this study, we present detailed investigations by confocal Raman and Drude model analysis of the material's changes and improvements, as it transitioned from 3D graphite to 2D graphene. Besides Raman spectral recording, which can detect single, a few, and multi-layers of graphene, confocal Raman mapping allows distinction of such domains and direct visualization of material inhomogeneity. Moreover, far-infrared transmittance measurements, which are related to electrical conductivity, demonstrate a distinct increase of conductivity with …

Sample Size Estimation For Linear Mixed Models With Dependent End Points, Michael Nsiah-Nimo

Open Access Theses & Dissertations

The primary objective is sample size estimation in linear mixed model settings. Sample size estimation is an important component of planning a well thought out scientific experiment. Whenever sample size estimation is performed, taking into account a priori model based inferences will provide a sample size estimate that will achieve the desired power without inflating the type I error rate of the study.

One common practice is a traditional approach cited in the literature that uses the largest sample size after you Bonferroni the type I error rate to estimate sample sizes as such. We are going to take into …

Evaluating Binary Splits On Nominal Inputs, Isaac Xoese Ocloo

Open Access Theses & Dissertations

The maximally selected statistic approach in building tree models is shown to be a cause of variable selection bias. In this study we propose three methods to solve this problem in building regression trees with nominal predictor variables. Out of the three methods

proposed we explored only two in detail and defer one for further research. We developed an exact method to compute the p-value corresponding to the maximized splitting statistic in regression trees for nominal predictor variables with at most 10 distinct levels and a

method to estimate the best cutoff point as a parameter in a parametric nonlinear …

Compressive Vector Reconstruction: Hypothesis For Blind Image Deconvolution, Alonso Orea Amador

Open Access Theses & Dissertations

Alternative imaging devices propose to acquire and compress images simultaneously. These devices are based on the compressive sensing (CS) theory. A reduction in the measurement required for reconstruction without a post-compression sub-system allows imaging devices to become simpler, smaller, and cheaper. In this research, we propose a new algorithm to compress and reconstruct blurred images for CS imaging devices. Blur effect in images is common due to relative motion, lens, limited aperture dimensions, lack of focus, and/or atmospheric turbulence. Our intention is to compress a blurred image with CS techniques and then reconstruct a blur-free version using the proposed algorithm. …

Cycling Of Gypsiferous White Sands Aerosols In The Shallow Critical Zone At White Mountain, New Mexico, Patrick Richard Rea

Open Access Theses & Dissertations

Dry deposition significantly affects evolution of the critical zone by nutrient supply and contributing to soil genesis. Dust influx and cycling in soils are difficult to quantify because dust sources can be chemically similar to local soils. White Sands, New Mexico, emits gypsum dust with a unique chemical and isotopic signature, providing an opportunity to investigate dust deposition and its movement in soils. This study evaluated the mobility of White Sands dust particles in the critical zone at White Mountain, New Mexico, a highland 100 km downwind. Four soil profiles were collected over limestone, igneous, mixed limestone and dolostone, and …

Household Water Consumption In El Paso, Texas: Perceptions And Behaviors Towards Water In Urban Households, Diego Armando Sanchez

Open Access Theses & Dissertations

The main objective of this research is to determine perceptions and behaviors of household water consumption and to examine how those may affect the patterns of water use and consumption levels in households of El Paso, Texas.

I conducted a qualitative exploratory research to collect important data that is often left unexplored in quantitative research. The collected data is a mixture of qualitative and quantitative. I do report the quantitative data using descriptive statistics, which help the reader see basic patterns in the study.

Qualitative data can help to understand the reasons that impact water consumption perceptions and behaviors in …

Renewable Energy Systems Optimization Using Monte Carlo Simulation And Evolutionary Algorithms, Nicolas Lopez

Open Access Theses & Dissertations

This Dissertation explores the Renewable Energy Integration Problem, and proposes a Genetic Algorithm embedded with a Monte Carlo simulation to solve large instances of the problem that are impractical to solve via full enumeration. The Renewable Energy Integration Problem is defined as finding the optimum set of components to supply the electric demand to a hybrid

microgrid. The components considered are solar panels, wind turbines, diesel generators, electric batteries, connections to the power grid and converters, which can be inverters and/or rectifiers. The methodology developed is explained as well as the combinatorial formulation. In addition, 2 case studies of a …

Data Analytics In Performance Of Kick-Out Distribution And Effectiveness In Senior Championship Football In Ireland, Roisin Donnelly, Darragh Daly

Journal Articles

n recent years, data analytics has been a growing phenomenon in many fields, including sport, and there has been an increased focus on how technology will impact the work of coaching andperformance professionals. This paper provides a reflection of the use of data analytics within the Gaelic Athletic Association’s (GAA) football coaching practice in Ireland, and evaluates for coaches how to enhance kick- out distribution and effectiveness. Specifically, the study aimed to dissect and analyse kick- out strategies to assess from a coaching perspective, the impact on distribution andeffectiveness. The research is a …

Genesis Of Unique Carbonate Fans In A Pre-Salt Reservoir Analogue, Indio Mountains, West Texas, Andre Llanos

Open Access Theses & Dissertations

Hydrocarbon exploration in pre-salt sediments of rift basins on the South Atlantic Margin have proven to be successful major oil plays. Located on the western margin of the rift, the Brazilian pre-salt reservoirs of the Santos, Campos, and Espirito Santo Basins are hosted by lacustrine/marine-influenced carbonates that comprise laterally extensive, laminated microbialite facies. The Indio Mountains, located in West Texas, provide well-exposed outcrops of Lower Cretaceous marine and non-marine rift basin strata that can be considered a reservoir-analog to the South Atlantic Margin pre-salt facies. Of particular interest for hydrocarbon exploration are unique carbonate features that have been discovered in …

Classification Of Radar Jammer Fm Signals Using A Neural Network Approach, Ariadna Estefania Mendoza

Open Access Theses & Dissertations

A Neural Network (NN) used to classify radar signals is proposed for the purpose of military survivability and lethality analysis. The goal of the NN is to correctly differentiate Frequency-Modulated (FM) signals from Additive White Gaussian Noise (AWGN) using limited signal pre-processing. The FM signals used to test the NN approach are the linear or chirp FM and the power-law FM. Preliminary simulations using the moments of the signals in the time and frequency domain yielded better results in the frequency domain, suggesting that time domain training would not be as effective frequency domain training. To test this hypoThesis, we …