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Articles 31 - 60 of 63
Full-Text Articles in Veterinary Medicine
Comparative Evaluation Of Indirect Enzyme Linked Immunosorbent Assay, Rose Bengal Plate Test, Microagglutination Test, And Polymerase Chain Reaction For Diagnosis Of Brucellosis In Buffaloes, Malik Raies Ul Islam, Mohinder Pratap Gupta, Pritam Kaur Sidhu, Gursimran Filia, Hari Mohan Saxena, Tawheed Ahmed Shafi
Comparative Evaluation Of Indirect Enzyme Linked Immunosorbent Assay, Rose Bengal Plate Test, Microagglutination Test, And Polymerase Chain Reaction For Diagnosis Of Brucellosis In Buffaloes, Malik Raies Ul Islam, Mohinder Pratap Gupta, Pritam Kaur Sidhu, Gursimran Filia, Hari Mohan Saxena, Tawheed Ahmed Shafi
Turkish Journal of Veterinary & Animal Sciences
In the present study, 178 blood samples from buffaloes were tested against indirect enzyme linked immunosorbent assay (I-ELISA), rose bengal plate test (RBPT), microagglutination test (MAT), modified microagglutination test (mMAT), and polymerase chain reaction (PCR) to select the most suitable test for efficient and effective diagnosis of bovine brucellosis. Win Episcope 2 software was used to determine the agreement between tests (kappa values at 95% confidence interval). I-ELISA was pair compared with all the other tests. Out of 178 samples, 102 were found positive by I-ELISA, 81 by RBPT, 85 by MAT, 79 by mMAT, and 68 by PCR. Substantial …
Comparative Diagnostic Methods For Canine Ehrlichiosis, Chetan Parmar, Riddhi Pednekar, Anant Jayraw, Mukulesh Gatne
Comparative Diagnostic Methods For Canine Ehrlichiosis, Chetan Parmar, Riddhi Pednekar, Anant Jayraw, Mukulesh Gatne
Turkish Journal of Veterinary & Animal Sciences
The efficacy of blood and buffy coat smear examination, commercially available dot-enzyme-linked immunosorbent assay (ELISA) kit, and nested polymerase chain reaction (PCR) was evaluated in the diagnosis of canine ehrlichiosis in 40 dogs exhibiting symptoms suggestive of the disease. The intracytoplasmic rickettsial organisms were seen in the smears of only 1 dog (2.5%) with an extremely low level of parasitemia. Nested PCR with 16S rRNA revealed characteristic 378 bp bands indicating positive reaction in 8 (20.0%) dogs and sera samples of 21 (52.5%) dogs revealed anti-Ehrlichia antibodies. The dog that revealed organisms in the blood also showed positive results with …
Determination Of Multidrug Resistance (Mdr1) Gene And Its Mutations In Dogs By Using Polymerase Chain Reaction, Sariya Asawakarn, Valaiporn Ruangchaiprakarn, Naparee Srisowanna, Leelawat Wongwan, Anusorn Kanuengthong, Gunnaporn Suriyaphol
Determination Of Multidrug Resistance (Mdr1) Gene And Its Mutations In Dogs By Using Polymerase Chain Reaction, Sariya Asawakarn, Valaiporn Ruangchaiprakarn, Naparee Srisowanna, Leelawat Wongwan, Anusorn Kanuengthong, Gunnaporn Suriyaphol
The Thai Journal of Veterinary Medicine
The objective of this study is to establish a polymerase chain reaction (PCR) laboratory for detection of multidrug resistance1 (MDR1) mutation in Thailand. Blood samples were collected from 67 dogs, namely 16 Collies, 9 Shetland Sheepdogs, 5 Border Collies, 6 Shih tzus, 13 German Shepherds and 18 mixed breeds. Genomic DNA was extracted and PCR was performed to amplify 341 and 577 bp fragments to detect homozygous wild-type (+/+) and homozygous mutant (-/-), respectively. For the heterozygous mutant (+/-), both PCR fragments would be presented. An analysis of DNA sequences encompassing the 4-base pair deletion in the coding region of …
Prevalence Of Escherichia Coli O157 In Red Meat And Meat Products Determined By Vidas Ecpt And Lightcycler Pcr, Seran Temelli̇, Ayşe Gül Eyi̇gör, Şahsene Anar
Prevalence Of Escherichia Coli O157 In Red Meat And Meat Products Determined By Vidas Ecpt And Lightcycler Pcr, Seran Temelli̇, Ayşe Gül Eyi̇gör, Şahsene Anar
Turkish Journal of Veterinary & Animal Sciences
This study aimed to determine the prevalence of Escherichia coli O157 in retail red meat and meat products with the Vitek Immunodiagnostic Assay System, including H7 Escherichia coli phage technology (VIDAS ECPT), and a real-time polymerase chain reaction system (LightCycler PCR; LCPCR). A total of 106 red meat and meat product samples were analyzed with VIDAS ECPT and LCPCR. VIDAS ECPT presumptive positive samples were subjected to VIDAS Immuno Concentration E. coli O157, followed by culture and serology. Among the 27 out of 72 (37.50%) red meat samples and 3 out of 34 (8.82%) red meat products that tested positive …
Toxinotyping Of Clostridium Perfringens Isolates By Elisa And Pcr From Lambs Suspected Of Enterotoxemia, Hasan Hüseyi̇n Hadi̇mli̇, Osman Ergani̇ş, Zafer Sayin, Zeki̇ Aras
Toxinotyping Of Clostridium Perfringens Isolates By Elisa And Pcr From Lambs Suspected Of Enterotoxemia, Hasan Hüseyi̇n Hadi̇mli̇, Osman Ergani̇ş, Zafer Sayin, Zeki̇ Aras
Turkish Journal of Veterinary & Animal Sciences
Clostridium perfringens is a gram-positive, anaerobic bacterium that causes a wide range of diseases in humans and animals. The purposes of this study were to determine, using the enzyme-linked immunosorbent assay (ELISA), the clostridial toxins in intestinal samples of lambs with suspected enterotoxemia; to conduct molecular typing of C. perfringens isolates; and to investigate the presence of the genes of 4 major toxins (\alpha, \beta, \epsilon, and \iota) in the isolates by polymerase chain reaction (PCR). According to the ELISA results, clostridial toxins were determined in 32 intestinal samples. Of the samples, 13 (40.62%) were C. perfringens type A, 1 …
The Virulence Of Thai Isolated Mycoplasma Gallisepticum In Challenged Embryonated Eggs, Somsak Pakpinyo, Suwarak Wanaratana, Sarawoot Mooljuntee
The Virulence Of Thai Isolated Mycoplasma Gallisepticum In Challenged Embryonated Eggs, Somsak Pakpinyo, Suwarak Wanaratana, Sarawoot Mooljuntee
The Thai Journal of Veterinary Medicine
This study was to investigate the virulence and pathogenicity of Thai isolated Mycoplasma gallisepticum (MG)inoculated into embryonated eggs and the chance of virulence study in embryonated eggs instead of experimentalchickens. One hundred and twenty eight-day-old embryonated eggs were divided into 4 groups as follows. Group 1consisting of 12 eggs served as sham negative control and was inoculated with 0.1 ml of broth into yolk sac. Group 2(2.1, 2.2 and 2.3), 3 (3.1, 3.2 and 3.3) and 4 (4.1, 4.2 and 4.3) were inoculated with 0.1 ml of MG strains F, 6/85 and Thaiisolated into yolk sacs, respectively. Each subgroup consisted …
First Report Of Fasciola Hepatica In Equus Caballus Host Species From Tunisia Based On The Ribosomal Internal Transcribed Spacer Regions, Nabil Amor, Sarra Farjallah, Khaled Said, Badreddine Ben Slimane
First Report Of Fasciola Hepatica In Equus Caballus Host Species From Tunisia Based On The Ribosomal Internal Transcribed Spacer Regions, Nabil Amor, Sarra Farjallah, Khaled Said, Badreddine Ben Slimane
Turkish Journal of Veterinary & Animal Sciences
Fasciolosis caused by Fasciola spp. (Platyhelminthes: Trematoda: Digenea) is considered the most important helminth infection of ruminants in tropical countries, causing considerable socioeconomic problems. Samples identified morphologically as Fasciola sp. in Equus caballus host species from the city of Tunis (north of Tunisia) were genetically characterized, for the first time, by sequences of the 1st (ITS-1), the 5.8S and 2nd (ITS-2) Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA). Comparison of the ITS of the Tunisian samples with sequences of Fasciola spp. from GenBank showed that the specimens examined had sequences identical to those of F. hepatica sequences selected …
Maedi-Visna Virus Infection In A Merino Lamb With Nervous Signs, Ahmet Akkoç, Meri̇ç Kocatürk, Ayli̇n Alasonyalilar Demi̇rer, Sezgi̇n Şentürk, Giacomo Renzoni, Silvia Preziuso
Maedi-Visna Virus Infection In A Merino Lamb With Nervous Signs, Ahmet Akkoç, Meri̇ç Kocatürk, Ayli̇n Alasonyalilar Demi̇rer, Sezgi̇n Şentürk, Giacomo Renzoni, Silvia Preziuso
Turkish Journal of Veterinary & Animal Sciences
A Maedi-Visna virus (MVV) infection was diagnosed in a 10-month-old lamb from a Turkish flock. The animal suddenly showed nervous signs and died within 2 days. At necropsy, different pulmonary and nervous lesions were observed. A Coenurus cerebralis cyst was also present in the right-brain hemisphere. Severe nonpurulent meningoencephalitis and lymphoproliferative pneumonia were detected by histopathology. MVV proviral DNA was demonstrated by polymerase chain reaction (PCR) in formalin-fixed and paraffin-embedded samples collected from the brain and the lungs. This unusual report in a young animal confirms that not only adult sheep but also lambs can develop MVV disease. The hypothesis …
Disseminated Tuberculosis In Captive Malayan Tapir (Tapirus Indicus), Theerayuth Kaewamatawong, Wijit Banlunara, Anudep Rungsipipat, Nopadon Pirarat, Suphasawatt Puranaveja, Angkana Sommanustweechai
Disseminated Tuberculosis In Captive Malayan Tapir (Tapirus Indicus), Theerayuth Kaewamatawong, Wijit Banlunara, Anudep Rungsipipat, Nopadon Pirarat, Suphasawatt Puranaveja, Angkana Sommanustweechai
The Thai Journal of Veterinary Medicine
Tuberculosis caused by Mycobacterium tuberculosis is an important zoonotic disease that concerns the survival of endangered species and public health aspects. A 17-year-old, female Malayan tapir from a zoo in Thailand died with a sign of panting after anesthesia for the treatment of her oral problems. Grossly, multiple caseous white nodules were scattered throughout the parenchyma and bulged from the capsular surface in affected organs such as lung, lymph node and liver. Histologic examination revealed multifocal to coalescing granulomas characterized by caseous necrotic center, loosely organized inflammatory zone with low numbers of Langhans’s giant cells and thin fibrotic zone. A …
Detection Of Necrotizing Hepatopancreatitis (Nhp) In Wild Shrimp From Laguna Madre, Mexico By A Multiplex Polymerase Chain Reaction, Gabriel Aguirre-Guzman, Jesus Genaro Sanchez-Martinez, Roberto Pérez-Castañeda, Rafael Orta-Rodriguez
Detection Of Necrotizing Hepatopancreatitis (Nhp) In Wild Shrimp From Laguna Madre, Mexico By A Multiplex Polymerase Chain Reaction, Gabriel Aguirre-Guzman, Jesus Genaro Sanchez-Martinez, Roberto Pérez-Castañeda, Rafael Orta-Rodriguez
The Thai Journal of Veterinary Medicine
The Laguna Madre in Mexico is a nursery, growth, and refuge area for shrimp and other wildlife species. These organisms support an important fishery for local industry (third place in Mexico), which may suffer diseases that are few studied. Wild shrimp were randomly collected from two sampling stations (Carrizal and Carbonera) once a month for one year (September 2007 to September 2008) to screen for virus and bacteria. The shrimp were taxonomically identified, organized in pools of three organisms per species and analyzed for the presence of WSSV, IHHNV, NHP and HPV by a commercial multiplex PCR kit DiagXotics Inc. …
Investigation Of Antimicrobial Susceptibility For Enterococci Isolated From Cats And Dogs And The Determination Of Resistance Genes By Polymerase Chain Reaction, Süheyla Türkyilmaz, Vasfi̇ye Erdem, Bülent Bozdoğan
Investigation Of Antimicrobial Susceptibility For Enterococci Isolated From Cats And Dogs And The Determination Of Resistance Genes By Polymerase Chain Reaction, Süheyla Türkyilmaz, Vasfi̇ye Erdem, Bülent Bozdoğan
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to investigate the antimicrobial susceptibility and to determinate the resistance genes by Polymerase Chain Reaction (PCR) among enterococci isolated from healthy cats and dogs in İzmir, Turkey. From a total of 114 rectal swabs, 91 (31 cats and 60 dogs) stains were isolated as enterococci. Twenty eight isolates were identified as Enterococcus faecalis and 63 as Enterococcus faecium with the API 20 IDStrep system. Antimicrobial susceptibilities were determined by disc diffusion. Susceptibility rates of all enterococci strains were found as 100.0%, 95.6%, 94.5%, 87.9%, 84.6%, 30.8%, 29.7%, and 0.0% for teicoplanin, penicillin, vancomycine, chloramphenicol, …
Surveillance Of Mycoplasma Synoviae Infection In Mixed Thai Native Chickens In The Area Of Nakornpathom Province, Somsak Pakpinyo, Somkid Khanda, Supanat Boonyapisitsopa
Surveillance Of Mycoplasma Synoviae Infection In Mixed Thai Native Chickens In The Area Of Nakornpathom Province, Somsak Pakpinyo, Somkid Khanda, Supanat Boonyapisitsopa
The Thai Journal of Veterinary Medicine
This study was to conduct a surveillance of Mycoplasma synoviae (MS) infection in mixed Thai native chickens. Samples were submitted from 30 mixed Thai native chicken flocks, 15 birds per flock, aged between 1-4.5 months in the area of Nakornpathom province in the September 2005-October 2006 period. Each bird was bled for MS serology by serum plate agglutination (SPA) and enzyme linked immunosorbent assay (ELISA) test kits and swabbed for MS antigen detection by polymerase chain reaction (PCR) technique. Results revealed that the positive reactors detected by the SPA test, ELISA and PCR procedure were 12, 9 and 8 flocks, …
The Outbreak Of Koi Herpesvirus (Khv) In Koi (Cyprinus Carpio Koi) From Chiang Mai Province, Thailand, Surachai Pikulkaew, Tongkorn Meeyam, Wijit Banlunara
The Outbreak Of Koi Herpesvirus (Khv) In Koi (Cyprinus Carpio Koi) From Chiang Mai Province, Thailand, Surachai Pikulkaew, Tongkorn Meeyam, Wijit Banlunara
The Thai Journal of Veterinary Medicine
Twenty-two sick koi with cumulative mortality showed clinical signs of depression, brachial hemorrhage and necrosis, focal hemorrhage, pale patches and ulcers on the skin. Clinical examination showed the heavy infection by opportunistic organisms such as Dactylogyrus sp., Gyrodactylus sp. and Saprolegnia sp. of the skin, gill and fins. Histopathologically, the gill showed severe acute diffuse necrosis of the branchial epithelial cells and severe diffuse lymphocytic-monocytic interstitial nephritis with necrosis of the tubular epithelial cells were predominantly found in most sick fish. The presence of homogeneous pale basophilic intranuclear viral inclusions was observed. Transmission electron microscopy showed herpesvirus-like particles in the …
Detection Of Bovine Tuberculosis By Tuberculin Test And Polymerase Chain Reaction In Van, Turkey, Hasan Solmaz, Zi̇ya İlhan, Abdulbaki̇ Aksakal, Ti̇mur Gülhan, İsmai̇l Hakki Eki̇n
Detection Of Bovine Tuberculosis By Tuberculin Test And Polymerase Chain Reaction In Van, Turkey, Hasan Solmaz, Zi̇ya İlhan, Abdulbaki̇ Aksakal, Ti̇mur Gülhan, İsmai̇l Hakki Eki̇n
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to identify the presence and prevalence of bovine tuberculosis in the eastern Anatolian border province of Van and its villages and to collect the preliminary data required for programs for the prevention and control of this zoonotic disease. The tuberculin test was performed on 210 cattle. Nasal swab and milk samples were obtained from the animals tested. Three cattle that had a positive tuberculin test also tested positive for the DNA target, a 580-bp fragment of IS6110 specific for members of the Mycobacterium tuberculosis complex. This fragment was also recovered from milk taken from …
Detection Of Bordetella Avium By Polymerase Chain Reaction In The Lungs And Tracheas Of Turkeys With Pneumonia, Süheyla Türkyilmaz, Sevi̇n Kirdar, Fulya Ocak, Şebnur Hazimoğlu
Detection Of Bordetella Avium By Polymerase Chain Reaction In The Lungs And Tracheas Of Turkeys With Pneumonia, Süheyla Türkyilmaz, Sevi̇n Kirdar, Fulya Ocak, Şebnur Hazimoğlu
Turkish Journal of Veterinary & Animal Sciences
Lung, trachea, and serum samples obtained from turkeys showing clinical signs of respiratory disease were examined for the presence of Bordetella avium infection (bordetellosis) using culture, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR). The material of this study consisted of 2 live turkeys at the age of 24 weeks with respiratory distress, nasal discharge, and cough that had been brought to the Microbiology Laboratory of the Faculty of Veterinary Medicine, Adnan Menderes University. The results of the study show that B. avium, the causative agent of bordetellosis, was not isolated from either the lung or trachea, while both …
Investigation Of Chicken Infectious Anemia Virus Infection By Pcr And Elisa In Chicken Flocks, H. Hüseyi̇n Hadi̇mli̇, Osman Ergani̇ş, Leyla Güler, Uçkun Sai̇t Uçan
Investigation Of Chicken Infectious Anemia Virus Infection By Pcr And Elisa In Chicken Flocks, H. Hüseyi̇n Hadi̇mli̇, Osman Ergani̇ş, Leyla Güler, Uçkun Sai̇t Uçan
Turkish Journal of Veterinary & Animal Sciences
Presence of the chicken infectious anemia virus (CIAV) infection in chicken flocks in some provinces of Turkey was investigated by ELISA and PCR in this study. From 38 flocks (16 commercial layer, 10 commercial broilers, 4 breeder layers and 8 breeder broilers), 922 serum samples were tested for CIAV-specific antibodies using a commercially available competitive ELISA. CIAV antibodies were positive in 609 (66.0%) sera from 34 flocks (89.5%). A total of 95 thymus samples from 25 flocks, 57 samples from 15 commercial layers, and 38 samples from 10 commercial broiler flocks were tested by PCR. In 53 (55.8%) thymus samples …
Detection Of Coxiella Burnetii In Cattle By Pcr, Şükrü Kirkan, Osman Kaya, Serten Tekbiyik, Uğur Parin
Detection Of Coxiella Burnetii In Cattle By Pcr, Şükrü Kirkan, Osman Kaya, Serten Tekbiyik, Uğur Parin
Turkish Journal of Veterinary & Animal Sciences
This study is aimed to detect Coxiella burnetii in cattle by Polymerase Chain Reaction (PCR). A total of 138 cattle blood samples were collected from 8 farms and then examined in laboratory conditions. The examination of Coxiella burnetii was carried out by PCR with specific primers. In this study, a total of 6 (4.3%) cattle serum samples were found PCR positive for Coxiella burnetii. This result proves that cattle are an important reservoir of Coxiella burnetii infection.
Development Of An Assay To Determine Single Nucleotide Polymorphisms In The Prion Gene For The Genetic Diagnosis Of Relative Susceptibility To Classical Scrapie In Sheep, Mary Lynn Johnson, Jessica M. Evoniuk, Charles L. Stoltenow, Katherine I. O'Rourke, Dale A. Redmer
Development Of An Assay To Determine Single Nucleotide Polymorphisms In The Prion Gene For The Genetic Diagnosis Of Relative Susceptibility To Classical Scrapie In Sheep, Mary Lynn Johnson, Jessica M. Evoniuk, Charles L. Stoltenow, Katherine I. O'Rourke, Dale A. Redmer
Zoonotics and Wildlife Disease: Publications
The objective of this study was to develop a reliable Taqman® 5' Nuclease Assay for genotyping sheep for scrapie susceptibility. The sheep prion gene contains 2 single nucleotide polymorphisms (SNPs) that may mediate resistance to classical scrapie, one at codon 136, alanine (A) or valine (V), and another at codon 171, arginine (R) or glutamine (Q). The R allele appears to confer resistance to classical scrapie, with the AA136 RR171 genotype the most resistant to scrapie and QR171 only rarely infected in the US sheep population. The Assays by DesignSM protocol was used for development of …
Detection Of The Eaea Gene In Escherichia Coli From Chickens By Polymerase Chain Reaction, Ayşe Kiliç, Hasan Basri̇ Ertaş, Adi̇le Muz, Gökben Özbey, Hakan Kalender
Detection Of The Eaea Gene In Escherichia Coli From Chickens By Polymerase Chain Reaction, Ayşe Kiliç, Hasan Basri̇ Ertaş, Adi̇le Muz, Gökben Özbey, Hakan Kalender
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to isolate Escherichia coli from chickens and to determine the presence of the eaeA gene, a virulence factor detected in E. coli, in the isolates by polymerase chain reaction (PCR). Different chicken organs were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. E. coli was isolated from 48% (48/100) of the samples. DNA was extracted from these isolates and was amplified by PCR, using a pair of primers derived from the eaeA (virulence) gene. In the agarose gel examination of PCR products, 48% (48/100) of the isolates were determined …
Isolation Of Salmonella Spp. In Camel Sausages From Retail Markets In Aydın, Turkey, And Polymerase Chain Reaction (Pcr) Confirmation, Gökben Özbey, Fi̇li̇z Kök, Adi̇le Muz
Isolation Of Salmonella Spp. In Camel Sausages From Retail Markets In Aydın, Turkey, And Polymerase Chain Reaction (Pcr) Confirmation, Gökben Özbey, Fi̇li̇z Kök, Adi̇le Muz
Turkish Journal of Veterinary & Animal Sciences
A total of 100 camel sausage samples from different retail markets in Aydın, located in southwest Turkey, were examined for the presence of Salmonella spp. by culture method. After sausage samples was preenriched in buffered peptone water and incubated 18-20 h at 37 °C. were transferred to Rappaport-Vassiliadis broth and Selenite broth, respectively, and incubated at 42 and 37 °C, respectively. After 24 and 48 h of incubation, one loopful from each of the enriched broths was streaked onto plates of Salmonella-Shigella agar and xylose lysine deoxycholate agar and incubated at 37 °C for 24 h. A 572-bp fragment of …
Identification Of Meat Species By Polymerase Chain Reaction (Pcr) Technique, O. İrfan İlhak, Ali̇ Arslan
Identification Of Meat Species By Polymerase Chain Reaction (Pcr) Technique, O. İrfan İlhak, Ali̇ Arslan
Turkish Journal of Veterinary & Animal Sciences
The origin of horse, dog, cat, bovine, sheep, porcine, and goat meat was determined by the polymerase chain reaction (PCR) technique, using species-specific primers. Test mixtures of meat were prepared by adding 5%, 2.5%, 1%, 0.5%, and 0.1% levels of pork, horse, cat, or dog meat to beef, sheep, and goat meat. Samples taken from those combinations were analyzed by PCR for species determination. Mitochondrial DNA (mt DNA) fragments of 439, 322, 274, 271, 225, 212, and 157 bp for horse, dog, cat, bovine, sheep, porcine, and goat meat, respectively, were amplified. PCR was conducted at 30 cycles for mixtures …
Laboratory Diagnosis Of Mycoplasma Gallisepticum (Mg) Infection In Experimental Layer Chicken Receiving Mg Vaccines And Mg Organisms, Somsak Pakpinyo, Pinyo Pitayachamrat, Seubchat Saccavadit, Than Santaswang, Achara Tawatsin, Jiroj Sasipreeyajan
Laboratory Diagnosis Of Mycoplasma Gallisepticum (Mg) Infection In Experimental Layer Chicken Receiving Mg Vaccines And Mg Organisms, Somsak Pakpinyo, Pinyo Pitayachamrat, Seubchat Saccavadit, Than Santaswang, Achara Tawatsin, Jiroj Sasipreeyajan
The Thai Journal of Veterinary Medicine
This experiment was designed to study the results of laboratory diagnosis of Mycoplasma gallisepticum (MG) infection in layer chicken receiving MG vaccines and MG organisms. One hundred and twenty 1-day-old-malelayer chickens were raised in separate isolation rooms and equally allocated into 4 separated groups. When 4 weeks old, groups 1, 2, 3 and 4 served as a negative control, a MG commercially inactivated vaccine group, a MG commercially live vaccine group and a MG infected group, respectively. In each group, ten birds were swabbed and bled when 4, 5, 6, 7, 8, 9 and 11 weeks old. Swab samples were …
Comparison Of Polymerase Chain Reaction And Conventional Methods For The Diagnosis Of Listeria Monocytogenes In Stuffed Mussels, Ergün Ö. Göksoy, Şükrü Kirkan, Osman Kaya
Comparison Of Polymerase Chain Reaction And Conventional Methods For The Diagnosis Of Listeria Monocytogenes In Stuffed Mussels, Ergün Ö. Göksoy, Şükrü Kirkan, Osman Kaya
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to evaluate the role of stuffed mussel as a source of Listeria monocytogenes. Polymerase chain reaction is a rapid procedure with both sensitivity and specificity for quick detection and identification of L. monocytogenes. A total of 50 mussel samples were investigated for L. monocytogenes. L. monocytogenes was not identified by the conventional method. However, PCR amplification products demonstrated that 5 out of 50 samples showed positive reactions with L. monocytogenes. The PCR positive samples showed specific amplification at approximately 343 bp for L. monocytogenes.
Characterization Of Footrot Bacteria Dichelobacter Nodosus Using Pcr Amplification And Dna Sequence Analysis, Ifakat Tülay Çağatay, Jon G. H. Hickford
Characterization Of Footrot Bacteria Dichelobacter Nodosus Using Pcr Amplification And Dna Sequence Analysis, Ifakat Tülay Çağatay, Jon G. H. Hickford
Turkish Journal of Veterinary & Animal Sciences
Dichelobacter nodosus is an essential causative agent of footrot in ruminants, particularly in sheep, goats and cattle. In this study, more than 100 footrot samples were collected from 4 different farming regions in New Zealand (NZ). Selective media were chosen and isolation and routine growth conditions were optimized for NZ D. nodosus serotypes. Approximately 1000 primary plates were anaerobically subcultured several times and examined with Gram staining in order to detect single colonies of D. nodosus. Both the variable region and a part of the conserved region of fimbrial subunit gene (fimA) were amplified from bacterial DNA using polymerase chain …
Identification Of Vibrio Anguillarum By Pcr (Rpon Gene) Associated With Vibriosis In Marine Fish In Turkey, Di̇dem Demi̇rcan, Akin Candan
Identification Of Vibrio Anguillarum By Pcr (Rpon Gene) Associated With Vibriosis In Marine Fish In Turkey, Di̇dem Demi̇rcan, Akin Candan
Turkish Journal of Veterinary & Animal Sciences
The main causative agent of vibriosis is Vibrio anguillarum. In this study, 33 V. anguillarum strains were isolated from the internal organs of marine fish showing typical clinical signs of vibriosis, and these strains were identified by biochemical tests. For comparison of these results, API 20E and BIONOR Mono-Va agglutination kits were used. Finally strains were amplified by PCR using the rpoN gene. Although the phenotypic properties of V. anguillarum strains varied, the rpoN gene from chromosomal DNA was amplified in all strains. In conclusion, this gene could be used in the diagnosis of V. anguillarum strains isolated in Turkey.
Detection Of Listeria Monocytogenes By Using Pcr In Helix Pomatia, Şükrü Kirkan, Ergün Ö. Göksoy, Osman Kaya
Detection Of Listeria Monocytogenes By Using Pcr In Helix Pomatia, Şükrü Kirkan, Ergün Ö. Göksoy, Osman Kaya
Turkish Journal of Veterinary & Animal Sciences
The detection of Listeria monocytogenes in Helix pomatia, both raw and cooked is presented here. Polymerase chain reaction (PCR) is a rapid procedure with both sensitivity and specificity for quick detection and identification of Listeria monocytogenes from raw and cooked Helix pomatia. A total of 30 bags (10 g each) of H. pomatia samples were investigated for the Listeria monocytogenes inlB gene with the PCR method. PCR amplification products demonstrated that 18 of the 30 samples showed positive reactions to Listeria monocytogenes in the PCR. All PCR positive samples showed specific amplification of the 343 bp fragment for Listeria monocytogenes.
Isolation Of Arcanobacterium (Actinomyces) Pyogenes From Abscessed Cattle Kidney And Identification By Pcr, Hasan Basri̇ Ertaş, Ayşe Kiliç, Gökben Özbey, Adi̇le Muz
Isolation Of Arcanobacterium (Actinomyces) Pyogenes From Abscessed Cattle Kidney And Identification By Pcr, Hasan Basri̇ Ertaş, Ayşe Kiliç, Gökben Özbey, Adi̇le Muz
Turkish Journal of Veterinary & Animal Sciences
In this study, the presence of Arcanobacterium spp., which causes a variety of purulent infections involving the skin, joints and visceral organs, was investigated in abscessed kidney samples of cattle. A total of 500 cattle were examined at postmortem and 100 samples with abscess were collected. Arcanobacterium pyogenes was isolated in 40 (40%) of the samples examined. DNA extracted from the isolates were amplified by Polymerase Chain Reaction (PCR) using specific primers derived from plo gene of A. pyogenes and all the isolates were determined to be positive by PCR. It was concluded that PCR employed in this study may …
Isolation Of Clostridium Perfringens From Chickens And Detection Of The Alpha Toxin Gene By Polymerase Chain Reaction (Pcr), Hakan Kalender, Hasan Basri̇ Ertaş
Isolation Of Clostridium Perfringens From Chickens And Detection Of The Alpha Toxin Gene By Polymerase Chain Reaction (Pcr), Hakan Kalender, Hasan Basri̇ Ertaş
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to isolate Clostridium perfringens from chickens and to detect the gene encoding the alpha toxin produced by all types of C. perfringens by polymerase chain reaction (PCR). Intestinal contents of 160 slaughtered chickens from 8 different farms in Elazığ province were analyzed. C. perfringens was isolated from 8 (5%) of the samples. DNA samples extracted from suspected isolates grown on selective agar were amplified by PCR using a pair of primers derived from the alpha toxin gene. All of 8 suspected isolates were found to be C. perfringens by conventional methods and PCR. Isolates were …
Identification Of Chicken Originated Campylobacter Coli And Campylobacter Jejuni By Polymerase Chain Reaction (Pcr), Hasan Basri̇ Ertaş, Burhan Çeti̇nkaya, Adi̇le Muz, Hasan Öngör
Identification Of Chicken Originated Campylobacter Coli And Campylobacter Jejuni By Polymerase Chain Reaction (Pcr), Hasan Basri̇ Ertaş, Burhan Çeti̇nkaya, Adi̇le Muz, Hasan Öngör
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to isolate Campylobacter species from the intestines and livers of chicken and to identify Campylobacter coli and Campylobacter jejuni by both conventional methods and Polymerase Chain Reaction (PCR). Four specific primers derived from the ceuE gene present in the genomes of C. coli and C. jejuni were used for PCR identification. In the examination of 150 intestine and liver samples by culture and PCR, 25 (16.6%) and 32 (21.3%) were identified as C. coli and C. jejuni, respectively. It was concluded that the PCR assay used in this study may successfully be applied for …
Detection Of Cattle Infected With Theileria Annulata In Fields By Nested Pcr, Ifat And Microscopic Examination Of Blood Smears, Zati̇ Vatansever, Serpi̇l Nalbantoğlu
Detection Of Cattle Infected With Theileria Annulata In Fields By Nested Pcr, Ifat And Microscopic Examination Of Blood Smears, Zati̇ Vatansever, Serpi̇l Nalbantoğlu
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to diagnose Theileria annulata infected cattle by microscopy, IFAT and nested PCR and to compare the sensitivity and specificity of these diagnostic methods. A total of 147 blood and sera samples were collected from healthy cattle in four localities of Polatlı district, Ankara, where tropical theileriosis is prevalent. Examination results revealed positivity rates of 31.3%, 44.9% and 61.2% for microscopy, IFAT and nested PCR, respectively. It was shown that nested PCR is more sensitive and specific when compared to microscopical examination and serological findings. It was also shown that nested PCR is more sensitive …