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Full-Text Articles in Veterinary Medicine

Vaccine Development From Serotypes O1, O2 And O78 Of Escherichia Coli Against Avian Colibacillosis: Layer Chickens, Osman Ergani̇ş, H. H. Hadi̇mli̇, Hasan Solmaz Jan 2002

Vaccine Development From Serotypes O1, O2 And O78 Of Escherichia Coli Against Avian Colibacillosis: Layer Chickens, Osman Ergani̇ş, H. H. Hadi̇mli̇, Hasan Solmaz

Turkish Journal of Veterinary & Animal Sciences

A trivalent Escherichia coli vaccine (TECA) was prepared from serotypes O1, O2 and O78 and one series of TECA was combined with an inactivated Newcastle Disease vaccine. TECA and combined vaccine were experimentally investigated for immunogenic effects in pullets and hens. This study was carried out at two stages consisting of rearing and laying periods: (1) A number of fourty chicks aged 15 days were subcutaneously vaccinated with 0.2 ml TECA . A similar group served as non-vaccinated controls. Half of the vaccinated group were revaccinated with 0.5 ml TECA at 71 days of age. The vaccinated and nonvaccinated pullets …


Immunoblot Analysis Of Campylobacters Of Chickens, Mehmet Akan, Jale Erdeğer, Zi̇ya İlhan Jan 2002

Immunoblot Analysis Of Campylobacters Of Chickens, Mehmet Akan, Jale Erdeğer, Zi̇ya İlhan

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine immunogenic structures by immunoblotting in thermophilic Campylobacter strains isolated from chickens. The bands obtained from C. jejuni strains were found to be distributed between 10.8 and 116.5 kD by SDS-PAGE analysis of whole cell proteins. The bands 78, 63, 52, 48.5, 43.5, 29.8, 25.6, 22, 18.5, 17.2 and 16.5 kD were detected as the major common bands in all C. jejuni strains. Electrophoretic analysis of C. coli strains revealed that protein band profiles ranged between 10.3 and 118 kD, and 76, 63-62, 52, 48.5, 44 and 30.5 kD bands were all major …


The Diagnosis Of Antibodies Against Newcastle Disease Virus By Hi And Elisa Tests In Vaccinated Chickens, Nejat Aydin, Hakan Yardimci, K. Semi̇h Gümüşsoy, Belgi̇n Erdem Jan 2001

The Diagnosis Of Antibodies Against Newcastle Disease Virus By Hi And Elisa Tests In Vaccinated Chickens, Nejat Aydin, Hakan Yardimci, K. Semi̇h Gümüşsoy, Belgi̇n Erdem

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine serum antibody levels against the Newcastle disease virus in vaccinated chicken flocks in addition to experimental animals by hemagglutination inhibition (HI)test and enzyme linked immunosorbent assay (ELISA) and compare the results. Methanol-precipitated antigen prepared from the LaSota strain of Newcastle disease virus was used with peroxidase-labelled rabbit anti-chicken IgG as a conjugate and ortho-phenylenediamine as a substrate in ELISA. The negative threshold was found to be an optical density of 0.19 (OD)at a 490-nm wavelength.In addition to the 50 experimental animals, a total of 504 chicken sera (broiler,layer and breeder flocks)collected from …


Pharmacokinetics And Tissue Levels Of Tilmicosin In Fowls, Oya Keleş, Tülay Bakirel, Süleyman Şener, Gül Baktir, Gürdal Dağoğlu, Oktay Özkan Jan 2001

Pharmacokinetics And Tissue Levels Of Tilmicosin In Fowls, Oya Keleş, Tülay Bakirel, Süleyman Şener, Gül Baktir, Gürdal Dağoğlu, Oktay Özkan

Turkish Journal of Veterinary & Animal Sciences

Tilmicosin is a long-acting macrolide antibiotic used in the treatment of respiratory disease. It was administered once (50 mg/kg b.wt) to fowls via the oral route for determination of kinetic properties and tissue levels. After oral administration, tilmicosin's pharmacokinetics conformed to a two compartment open model. Tilmicosin was slowly eliminated from the serum and lung with mean half-lives of 30.18 ± 2.38 and 75.74 ± 3.67 hours, respectively. The mean maximum concentration of the drug in the lungs was found to be 6.2 times greater that of serum. Serum and lung tilmicosin concentrations reached peak values 4.66 ± 2 and …


Diagnosis Of Lymphoid Leukosis (Ll) By Elisa (Enzyme-Linked Immunosorbent Assay) And Marek's Disease (Md) By If (Immunofluorescence) Test In Chickens, Selda Özbi̇lgi̇n, Ayşi̇n Şen, Mi̇hri̇ban Ülgen, K. Tayfun Çarli, Gürsel Sönmez, Özlem Özmen Jan 2001

Diagnosis Of Lymphoid Leukosis (Ll) By Elisa (Enzyme-Linked Immunosorbent Assay) And Marek's Disease (Md) By If (Immunofluorescence) Test In Chickens, Selda Özbi̇lgi̇n, Ayşi̇n Şen, Mi̇hri̇ban Ülgen, K. Tayfun Çarli, Gürsel Sönmez, Özlem Özmen

Turkish Journal of Veterinary & Animal Sciences

In this study, our aim was to diagnose lymphoid leukosis and Marek's disease, which are neoplastic diseases of chickens, by ELISA and Indirect Immunofluorescence Test (IIF), respectively. Samples (eggs and tissues) were selectively taken from the flocks suspected of having lymphoid leukosis and Marek's disease. The tissues were macroscopically and microscopically examined. Tissues belonging to some flocks showed typical lesions for lymphoid leukosis and Marek's disease. Seven broiler breede flocks and three commercial layer flocks were tested for avian leukosis virus (ALV) group specific antigen (p27) in the albumen. ALV p27 antigen was detected in five out of ten flocks. …


Morphology Of The Spinal Cord In The Chicken, Duck And Pigeon, R. Meri̇h Haziroğlu, İ. Önder Orhan, Di̇nçer Yildiz, M. Erdem Gülti̇ken Jan 2001

Morphology Of The Spinal Cord In The Chicken, Duck And Pigeon, R. Meri̇h Haziroğlu, İ. Önder Orhan, Di̇nçer Yildiz, M. Erdem Gülti̇ken

Turkish Journal of Veterinary & Animal Sciences

The morphology of the spinal cord in 15 chickens, 15 ducks and 15 pigeons obtained from the Ankara region were examined. The spinal cord consisted of 15 cervical, 7 thoracic, 14 lumbosacral and 5 coccygeal segments (a total of 41 segments) in the chickens; 15 cervical, 8 thoracic, 16 lumbosacral and 5 coccygeal segments (a total of 44 segments) in the ducks and 13 cervical, 6 thoracic, 13 lumbosacral and 5 coccygeal segments (a total of 37 segments) in the pigeons. The cervical enlargement was composed of cervical segments 13-15 and thoracic segments 1-2 in the chickens; cervical segments 12-15 …


Detection Of Antibodies Against Salmonella Enteritidis In Chicken Serum And Egg Yolk With Elisa, Gülay Altay, Hakan Yardimci Jan 2001

Detection Of Antibodies Against Salmonella Enteritidis In Chicken Serum And Egg Yolk With Elisa, Gülay Altay, Hakan Yardimci

Turkish Journal of Veterinary & Animal Sciences

A total of 473 sera from hens of different age groups and different breeds and a total of 430 eggs were collected from 11 breeders and egg producers in the districts of Adapazarı, Afyon, Ankara and Konya. Lipopolysaccharide (LPS), heat-extract (HE) and flagella antigens were tested in the standardizing ELISA technique. Flagella antigen was found useful in the screening of field samples. Of the 48 (10.2%) rapid plate agglutination positive sera, 7 were negative in ELISA. One hundred and seventy-three (36.6%) of the 473 sera and 76 (17.7%) of the 430 egg yolks were found to be positive with ELISA. …


Typing Of Salmonella Spp. Isolated From Chickens In Elazığ Region, Hakan Kalender, Adi̇le Muz Jan 1999

Typing Of Salmonella Spp. Isolated From Chickens In Elazığ Region, Hakan Kalender, Adi̇le Muz

Turkish Journal of Veterinary & Animal Sciences

In this study, 365 chickens slaughtered at a local abattoir and 162 chickens admitted to Elazığ Veterinary Control and Research Institute were investigated for Salmonellosis. Salmonella strains were identified and serogrouped. Antibiotic susceptibility tests were also performed. Fifty seven (10.81%) Salmonella strains were isolated from 527 chickens. Salmonella strains were serogrouped by coagglutination test and slide agglutination test. Of 57 strains, 53 were in group D1, 4 were in group B. Of 53 strains in group D1, 39 were serotyped as S. enteritidis and 14 as S. gallinarum. Four strains in group B were serotyped as S. typhimurium. In phagetyping …


Investigation Of Listeria Species In Chicken Body Parts And Samples Of Water Used For Washing Carcasses, Ali̇ Arslan, Zafer Gönülalan, Ahmet H. Di̇nçoğlu, Fi̇li̇z Kök Jan 1999

Investigation Of Listeria Species In Chicken Body Parts And Samples Of Water Used For Washing Carcasses, Ali̇ Arslan, Zafer Gönülalan, Ahmet H. Di̇nçoğlu, Fi̇li̇z Kök

Turkish Journal of Veterinary & Animal Sciences

In this study chicken body parts (leg, neck, wing, breast) and samples of water for washing carcasses, obtained from a slaughterhouse in Elazığ, were investigated in terms of listeria species. 20 samples were examined from each body part and carcass the water. L. monocytogenes, L. innocua and L. welshimeri were determined in 2, 7 and 4 leg samples; in 1, 3 and 5 wing samples and in 2, 5 and 4 water samples respectively. Only L. innocua was observed in 3 breast samples. L. monocytogenes and L. innocua were found in 3 and 2 leg samples respectively. In conclusion, it …


Pathological Findings In Caecal Coccidiosis Of Chickens, Ayhan Atasever, K. Semi̇h Gümüşsoy Jan 1999

Pathological Findings In Caecal Coccidiosis Of Chickens, Ayhan Atasever, K. Semi̇h Gümüşsoy

Turkish Journal of Veterinary & Animal Sciences

Caecal coccidiosis was determined in 12 white Leghorn chickens aged 18 weeks. Macroscopically, slight thickness, con-gestion and petechie were observed in the caecum. The lumen of caecum was full of blood and, necrotic areas were seen on the mucosal surface due to the damagae of villi epithelisl cells. Microscopically, it was observed that erythrocyte, epithelial cells and forms of parasites were present in the lumen of caecum due to the damage of villi epithelial cells. Lymphocyte, plasma cell and heterophil cell infiltrations were found in the propria mucosa as well as gangrenous typhilitis containing focal necrosis.