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2021

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Articles 121 - 150 of 556

Full-Text Articles in Veterinary Medicine

Successful Atac-Seq From Snap-Frozen Equine Tissues, Sichong Peng, Rebecca Bellone, Jessica L. Petersen, Theodore S. Kalbfleisch, Carrie J. Finno Jun 2021

Successful Atac-Seq From Snap-Frozen Equine Tissues, Sichong Peng, Rebecca Bellone, Jessica L. Petersen, Theodore S. Kalbfleisch, Carrie J. Finno

Veterinary Science Faculty Publications

An assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) has become an increasingly popular method to assess genome-wide chromatin accessibility in isolated nuclei from fresh tissues. However, many biobanks contain only snap-frozen tissue samples. While ATAC-seq has been applied to frozen brain tissues in human, its applicability in a wide variety of tissues in horse remains unclear. The Functional Annotation of Animal Genome (FAANG) project is an international collaboration aimed to provide high quality functional annotation of animal genomes. The equine FAANG initiative has generated a biobank of over 80 tissues from two reference female animals and experiments to begin …

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Attenuated Strain Of Cvb3 With A Mutation In The Car‑Interacting Region Protects Against Both Myocarditis And Pancreatitis, Ninaad Lasrado, Arunakumar Gangaplara, Chandirasegaran Massilamany, Rajkumar Arumugam, Allison Shelbourn, Mahima T. Rasquinha, Rakesh H. Basavalingappa, Gustavo Delhon, Shi-Hua Xiang, Asit K. Pattnaik, David Steffen, Jay Reddy Jun 2021

Attenuated Strain Of Cvb3 With A Mutation In The Car‑Interacting Region Protects Against Both Myocarditis And Pancreatitis, Ninaad Lasrado, Arunakumar Gangaplara, Chandirasegaran Massilamany, Rajkumar Arumugam, Allison Shelbourn, Mahima T. Rasquinha, Rakesh H. Basavalingappa, Gustavo Delhon, Shi-Hua Xiang, Asit K. Pattnaik, David Steffen, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

Coxsackievirus B3 (CVB3), is commonly implicated in myocarditis, which can lead to dilated cardiomyopathy, in addition to causing acute pancreatitis and meningitis. Yet, no vaccines are currently available to prevent this infection. Here, we describe the derivation of a live attenuated vaccine virus, termed mutant (Mt) 10, encoding a single amino acid substitution H790A within the viral protein 1, that prevents CVB3 infection in mice and protects from both myocarditis and pancreatitis in challenge studies. We noted that animals vaccinated with Mt 10 developed virus neutralizing antibodies, predominantly containing IgG2a and IgG2b, and to a lesser extent IgG3 and IgG1. …

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Contemporary Challenges And Opportunities For The Management Of Bird Damage At Field Crop Establishment, Christophe Sausse, Alice Baux, Michel Bertrand, Elsa Bonnaud, Sonia Canavelli, Alexandra Destrez, Page E. Klug, Lourdes Olivera, Ethel Rodriguez, Guilllermo Tellechea, Sebastian Zuil Jun 2021

Contemporary Challenges And Opportunities For The Management Of Bird Damage At Field Crop Establishment, Christophe Sausse, Alice Baux, Michel Bertrand, Elsa Bonnaud, Sonia Canavelli, Alexandra Destrez, Page E. Klug, Lourdes Olivera, Ethel Rodriguez, Guilllermo Tellechea, Sebastian Zuil

United States Department of Agriculture Wildlife Services: Staff Publications

Bird damage, from sowing to crop establishment, is an important issue for farmers in many parts of the world. However, reliable and cost-effective solutions remain elusive because management tools and research on the subject are limited. The spatial variability of damage across landscapes and the adaptative behaviour of birds create further challenges. Additionally, the issue must be tackled at the landscape scale and involve a variety of stakeholders with conflicting interests and objectives. We summarize some of the challenges and opportunities identified to face these difficulties and address four major research directions for operational solutions including 1) crop damage assessment, …

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Untangling A Cat’S Cradle: Diphacinone, Acetaminophen, Trauma, And Multiple Deaths In A Kentucky Cat Colony, Megan C. Romano, Uneeda K. Bryant, Lynne M. C. Cassone, Jennifer G. Janes, Michelle N. Helm, Kyle A. Francis Jun 2021

Untangling A Cat’S Cradle: Diphacinone, Acetaminophen, Trauma, And Multiple Deaths In A Kentucky Cat Colony, Megan C. Romano, Uneeda K. Bryant, Lynne M. C. Cassone, Jennifer G. Janes, Michelle N. Helm, Kyle A. Francis

Veterinary Diagnostic Laboratory Faculty Publications

This manuscript describes an animal cruelty investigation in which several cats from a feral colony were suspected of having been poisoned. Three of the cats were submitted for complete postmortem examination by a qualified veterinary pathologist, and appropriate toxicologic analyses were selected and interpreted by a qualified veterinary toxicologist. Diagnosis in poisoning cases requires communication and collaboration between the pathologists and the toxicologist. A truly definitive diagnosis is not always possible in poisoning cases, particularly in a forensic investigation – a court of law requires a higher standard to establish a cause of death than do routine diagnostic cases. This …

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Serological Survey Of Leptospira Infection In Arabian Horses In Poland, Bernard Wasiński, Katarzyna Paschalis-Trela, Jan Trela, Michał Czopowicz, Jerzy Kita, Monika Żychska, Anna Cywińska, Iwona Markowska-Daniel, Craig N. Carter, Lucjan Witkowski Jun 2021

Serological Survey Of Leptospira Infection In Arabian Horses In Poland, Bernard Wasiński, Katarzyna Paschalis-Trela, Jan Trela, Michał Czopowicz, Jerzy Kita, Monika Żychska, Anna Cywińska, Iwona Markowska-Daniel, Craig N. Carter, Lucjan Witkowski

Veterinary Diagnostic Laboratory Faculty Publications

Leptospirosis is one of the most common zoonotic infections worldwide, including in most livestock, some companion animals, horses, wildlife, and humans. Epidemiological estimation of its prevalence in all species is difficult due to the variety of clinical presentations and challenges regarding laboratory diagnosis. The purpose of this study was to measure the seroprevalence of leptospiral infection in Arabian horses kept in the largest breeding farms in Poland, representing over 15% of the Polish Arabian horse population. Leptospira antibodies were detected by MAT (cut-off 1:100) in 33.2% of serum samples (204 of 615 animals) (CI 95%: 29.6–37.0%), most frequently reacting with …

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Comparing Management Programs To Reduce Red–Tailed Hawk Collisions With Aircraft, Brian Washburn, Craig K. Pullins, Travis L. Guerrant, Gregory J. Martinelli, Scott F. Beckerman Jun 2021

Comparing Management Programs To Reduce Red–Tailed Hawk Collisions With Aircraft, Brian Washburn, Craig K. Pullins, Travis L. Guerrant, Gregory J. Martinelli, Scott F. Beckerman

United States Department of Agriculture Wildlife Services: Staff Publications

Wildlife-aircraft collisions (wildlife strikes) pose a serious safety risk to aircraft. Raptors (i.e., hawks and owls) are one of the most frequently struck guilds of birds within North America. Although raptors (most notably red-tailed hawks [Buteo jamaicensis]) are commonly managed at most airports and military bases, there is no scientific information available regarding comparisons of the efficacy of raptor management programs for reducing raptor-aircraft collisions. Therefore, we conducted a study to examine the efficacy of 2 raptor hazard management programs implemented at Chicago's O'Hare International Airport (ORD). The first raptor management program (Phase I) occurred during January 2010–June 2013 and …

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Retrospective Analysis Of The Incidence, Aetiology And Risk Factors Of Sepsis Based On Post-Mortem Examination Records In Ruminants, Batrisyia Syazana Faridon, Mohd Zamri-Saad, Annas Salleh Jun 2021

Retrospective Analysis Of The Incidence, Aetiology And Risk Factors Of Sepsis Based On Post-Mortem Examination Records In Ruminants, Batrisyia Syazana Faridon, Mohd Zamri-Saad, Annas Salleh

The Thai Journal of Veterinary Medicine

Sepsis is an important disease in both humans and animals. In animals, it is usually caused by septicaemia after infection and has not been well-studied. This article describes a retrospective analysis of sepsis cases in small and large ruminants submitted to the Post-Mortem Laboratory, Faculty of Veterinary Medicine, Universiti Putra Malaysia between the years 2007 and 2017. The incidence of sepsis was found to be at 40.5% in small ruminants and 38.6% in large ruminants. In both small and large ruminants, sepsis was mainly caused by bacterial infection, which was significantly (p<0.05) higher compared to parasitic and viral infections. Most cases of sepsis occur due to infections originating from the gastrointestinal tract and this is followed by the respiratory tract. Escherichia coli has been found to be the most common bacterial pathogen of sepsis in ruminants; significantly (p<0.05) higher compared to other bacterial pathogens like Klebsiella pneumoniae, Pasteurella sp., and Staphylococcus aureus. Sepsis involving E. coli has often resulted in pure isolation of the pathogen, thus being suggestive of a single infection. Underlying or concurrent infection is the most important risk factor leading to sepsis in ruminants, with significant difference (p<0.05) compared to other risk factors such as stress or malnutrition. Associations between the gastrointestinal system as the point of entry and sepsis caused by either E. coli or K. pneumoniae have been noted. This study provides an insight into a basic understanding of sepsis in ruminants.

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Investigation Of Avian Polyomavirus And Psittacine Beak And Feather Disease Virus In Parrots In Taiwan, Sheng-Chang Chung, Ching-Dong Chang, Perng-Chih Shen, Wei Lin, Chiu-Huang Ting, Hung-Yi Wu Jun 2021

Investigation Of Avian Polyomavirus And Psittacine Beak And Feather Disease Virus In Parrots In Taiwan, Sheng-Chang Chung, Ching-Dong Chang, Perng-Chih Shen, Wei Lin, Chiu-Huang Ting, Hung-Yi Wu

The Thai Journal of Veterinary Medicine

This study aimed to understand the related statistics of APV and PBFDV in parrots, such as the positive percentage and the distributions of various ages, seasons, breeds and areas, in order to obtain more information on the health care of captive parrots in Taiwan today. Blood samples were collected from parrots that were household pets and from commercial aviaries in the northern (e.g. Taipei), central (e.g. Taichung) and southern (e.g. Kaohsiung) Taiwan regions randomly. Collection targets were mainly small and medium species such as Psittacula krameri and others such as Psittacus erithacus. Sixty samples were collected every month and a …

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Administration Of The Α-Tocopherol For Repairing Testicle Histological Damage In Rats Exposed To Dioxin, Wurlina Wurlina, Imam Mustofa, Dewa Ketut Meles, Sri Mulyati, Desak Ketut Sekar Cempaka Putri, Niluh Suwasanti Jun 2021

Administration Of The Α-Tocopherol For Repairing Testicle Histological Damage In Rats Exposed To Dioxin, Wurlina Wurlina, Imam Mustofa, Dewa Ketut Meles, Sri Mulyati, Desak Ketut Sekar Cempaka Putri, Niluh Suwasanti

The Thai Journal of Veterinary Medicine

This study aims to determine the α-tocopherol effect on repairing histological damage to the testicles of rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin. The testicle histological damage and repairs were evaluated based on spermatogenic staging changes, seminiferous tubule diameter and the thickness of the epithelium. A total of 100 rats were divided into five groups. The control group (CG) rats only received a placebo. Meanwhile, in treatment groups, rats were exposed to dioxin 700 ng/kg/BW/day and four hours later they received a 0, 77, 140 and 259 α-tocopherol mg/kg/BW/day in the T0, T1, T2 and T3 groups, respectively. Dioxin exposure and α-tocopherol administration …

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Molecular Evidence Of Cryptosporidiosis In Farmed Blue Foxes (Vulpes Lagopus) And Raccoon Dogs (Nyctereutes Procyonoides) In Heilongjiang, China, Shakeel Hussain, Lixin Wang, Zhijun Hou Jun 2021

Molecular Evidence Of Cryptosporidiosis In Farmed Blue Foxes (Vulpes Lagopus) And Raccoon Dogs (Nyctereutes Procyonoides) In Heilongjiang, China, Shakeel Hussain, Lixin Wang, Zhijun Hou

The Thai Journal of Veterinary Medicine

Cryptosporidium, an important zoonotic protozoan, causes Cryptosporidiosis in humans as well as in animals. This study aimed to identify Cryptosporidiosis in farmed fur animals (Blue Fox and Raccoon Dogs). A total of 283 specimens (223 foxes, 60 Raccoon dogs) were screened through nested-PCR by targeting the actin gene. The results showed the overall prevalence of cryptosporidiosis was 9.1% where the prevalence in foxes and raccoon dogs was 9.1% and 8.3%, respectively. The prevalence in males (15%) was significantly higher than that in females (5.7%). The findings of this study portrayed new knowledge that foxes and raccoon dogs are likely to …

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Left Lateral Liver Lobe Torsion In A Siamese Cat, Dongwook Kim, Ki-Jeong Na, Dongwoo Chang, Gonhyung Kim Jun 2021

Left Lateral Liver Lobe Torsion In A Siamese Cat, Dongwook Kim, Ki-Jeong Na, Dongwoo Chang, Gonhyung Kim

The Thai Journal of Veterinary Medicine

A 6-year-old neutered male Siamese cat was presented with an acute onset of vomiting, anorexia and lethargy lasting for four days. An abdominal mass was palpated on physical examination. The cat showed severe anemia, elevated liver enzymes and hypoalbuminemia. Abdominal ultrasonography revealed a suspected hepatic mass without blood flow within the affected region. Computed tomography was performed to better visualize the abdominal cavity and blood flow. Prompt diagnosis of torsion of the left lateral liver lobe was made and immediate surgery for liver lobectomy was performed. Histopathological examination revealed no evidence of neoplasia. The cat remained clinically normal during the …

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Radiographic And Ultrasonographic Appearances Of Chronic Tenosynovitis Of The Lateral Extensor Tendon At The Right Tarsus In An Argentinian Polo Pony, Metha Chanda, Ananya Pongpradit, Wutthiwong Theerapan, Attapon Kamlangdee, Chanikarn Puangthong, Thapana Jarutummasiri Jun 2021

Radiographic And Ultrasonographic Appearances Of Chronic Tenosynovitis Of The Lateral Extensor Tendon At The Right Tarsus In An Argentinian Polo Pony, Metha Chanda, Ananya Pongpradit, Wutthiwong Theerapan, Attapon Kamlangdee, Chanikarn Puangthong, Thapana Jarutummasiri

The Thai Journal of Veterinary Medicine

A 19-year-old polo pony presented with mild lameness alongside a lateral protuberance of the right tarsus. Radiographic findings revealed various defects, including an osteochondral lesion of the medial trochlear ridge of the talus, a sclerotic lesion of the tarsometatarsal joint, a radiolucent area on the distal border of the fourth tarsal bone and an enthesophyte at the proximolateral aspect of the metatarsal bone. Ultrasonography demonstrated the interesting finding of synovial sheath thickening of the lateral extensor tendon with synovial fluid accumulation. In addition, the presence of neutrophils and a granular mucinous background, according to cytological analysis of the fluid, indicated …

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Alfaxalone Successfully Immobilizes Small Indian Mongooses (Urva Auropunctata): A Field Report, Caroline C. Sauvé, Yves Rondenay, Are R. Berentsen, Mel J. Rivera-Rodriguez, Patrick A. Leighton Jun 2021

Alfaxalone Successfully Immobilizes Small Indian Mongooses (Urva Auropunctata): A Field Report, Caroline C. Sauvé, Yves Rondenay, Are R. Berentsen, Mel J. Rivera-Rodriguez, Patrick A. Leighton

United States Department of Agriculture Wildlife Services: Staff Publications

We investigated intramuscular administration of alfaxalone (5.3–10.0 mg/kg) as an immobilizing agent in free-ranging small Indian mongooses (Urva auropunctata) on the island of St. Kitts, West Indies. From 5–11 January 2020, we successfully immobilized 10 of 11 mongooses. Alfaxalone provided rapid onset (median = 3 min) of immobilization. Mean duration of immobilization was 16.8 ± 3 min. Mean recovery time was 5.6 ± 1 min. There was no effect of dose administered on induction, anesthesia, or recovery times at the dose range used. We concluded that alfaxalone represents an effective alternative to dissociative agents for the immobilization of free-ranging mongooses. …

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Strategic Testing Approaches For Targeted Disease Monitoring Can Be Used To Inform Pandemic Decision-Making, James D. Nichols, Tiffany L. Bogich, Emily Howerton, Ottar N. Bjørnstad, Rebecca K. Borchering, Matthew Ferrari, Murali Haran, Christopher Jewell, Kim M. Pepin, William J.M. Probert, Juliet R.C. Pulliam, Michael C. Runge, Michael Tildesley, Cecile Viboud, Katriona Shea Jun 2021

Strategic Testing Approaches For Targeted Disease Monitoring Can Be Used To Inform Pandemic Decision-Making, James D. Nichols, Tiffany L. Bogich, Emily Howerton, Ottar N. Bjørnstad, Rebecca K. Borchering, Matthew Ferrari, Murali Haran, Christopher Jewell, Kim M. Pepin, William J.M. Probert, Juliet R.C. Pulliam, Michael C. Runge, Michael Tildesley, Cecile Viboud, Katriona Shea

United States Department of Agriculture Wildlife Services: Staff Publications

More than 1.6 million Servere Acute Respiratory Syndrome Cronovirus 2(SARS-COV-2)tests were administered daily in the United States at the peak of the epidemic, with a significant focus on individual treatment. Here, we show that objective-driven, strategic sampling designs and analyses can maximize information gain at the population level, which is necessary to increase situational awareness and predict, prepare for, and respond to a pandemic, while also continuing to inform individual treatment. By focusing on specific objectives such as individual treatment or disease prediction and control (e.g., via the collection of population- level statistics to inform lockdown measures or vaccine rollout) …

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An Evaluation Of Body-Grip Trap Trigger Configurations For Reducing River Otter Take Incidental To Beaver Trapping, Alec J. Sundelius, Nicholas Forman, Nathan M. Roberts, Shawn T. Rossler, David B. Ruid, Robert C. Willging Jun 2021

An Evaluation Of Body-Grip Trap Trigger Configurations For Reducing River Otter Take Incidental To Beaver Trapping, Alec J. Sundelius, Nicholas Forman, Nathan M. Roberts, Shawn T. Rossler, David B. Ruid, Robert C. Willging

United States Department of Agriculture Wildlife Services: Staff Publications

River otter (Lontra canadensis) populations in North America have been the focus of significant restoration efforts. Wildlife management agencies, concerned about the unintentional take of river otters incidental to beaver (Castor canadensis) trapping, may recommend techniques to avoid capturing river otters. River otter avoidance techniques that are ineffective or diminish trap performance for beavers are undesirable. We conducted a field evaluation in 2015 and 2016 in Wisconsin to assess how two trigger configurations (offset and center) on body-grip traps would affect the incidental capture rate of river otters during beaver trapping. We also evaluated effects of …

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Prevalence, Risk Factors And Antimicrobial Resistance Of Salmonella Infection In Turtles In Khon Kaen Province, Patchanee Sringam, Peerapol Sukon, Sarawut Sringam, Sunpetch Angkititrakul Jun 2021

Prevalence, Risk Factors And Antimicrobial Resistance Of Salmonella Infection In Turtles In Khon Kaen Province, Patchanee Sringam, Peerapol Sukon, Sarawut Sringam, Sunpetch Angkititrakul

The Thai Journal of Veterinary Medicine

Considering the impact of Salmonella in turtles and the role of turtles as a source of Salmonella outbreaks in humans, knowledge of Salmonella status in turtles is important. A cross-sectional study was conducted to investigate the prevalence, risk factors and antimicrobial resistance of Salmonella infections in turtles. Cloacal swabs were collected from 378 turtles in Turtle Villages located in Khon Kaen province of northeastern Thailand between January 2018 and April 2019. All samples were examined for Salmonella spp. isolation and identification by ISO 6597:2002. The overall prevalence of Salmonella in the turtles was 37.57% (142/378). Habitat, season and sex were …

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Expression Of Recombinant S2 Domain Of Spike Protein Of Porcine Epidemic Diarrhea Virus, Jiraporn Sritun, Natnaree Inthong, Siriluk Jala, Sakuna Phatthanakunanan, Kaitkanoke Sirinarumitr, Preeda Lertwatcharasarakul, Theerapol Sirinarumitr Jun 2021

Expression Of Recombinant S2 Domain Of Spike Protein Of Porcine Epidemic Diarrhea Virus, Jiraporn Sritun, Natnaree Inthong, Siriluk Jala, Sakuna Phatthanakunanan, Kaitkanoke Sirinarumitr, Preeda Lertwatcharasarakul, Theerapol Sirinarumitr

The Thai Journal of Veterinary Medicine

Porcine epidemic diarrhea virus (PEDV) is the causative agent of severe watery diarrhea in suckling piglets. PEDV outbreaks have been consistently reported in Europe, Asian countries and Thailand. The recombinant protein, especially the spike (S) protein, from field strain PEDV could be used to develop a sensitive diagnostic test or an effective vaccine. In this study, the recombinant S2 domain protein from the PEDV Thai isolate was expressed using the E. coli expression system. The full length S2 domain of the S gene of the PEDV G2b Thai isolate was amplified using a PCR technique and was cloned into the …

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Identification And Characterization Of A Novel Protective Antigen, Sesec_00396 Of Streptococcus Equi Ssp. Zooepidemicus, Li Yi, Weiping Yang, Jinpeng Li, Shaohui Wang, Yang Wang Jun 2021

Identification And Characterization Of A Novel Protective Antigen, Sesec_00396 Of Streptococcus Equi Ssp. Zooepidemicus, Li Yi, Weiping Yang, Jinpeng Li, Shaohui Wang, Yang Wang

The Thai Journal of Veterinary Medicine

Streptococcus equi ssp. zooepidemicus (SEZ) is responsible for a wide variety of infections in many animal species. Studies aimed at developing safe and effective vaccines against SEZ infections are thus of great interest. SeseC_00396 was a novel protein identified in the previous study. In this study, recombinant SeseC_00396 of SEZ was expressed and showed a strong immunoreactivity with mini-pig convalescent sera. Studies in mice have revealed that the recombinant protein induced a marked antibody response which was associated with protection against SEZ infection. The hyperimmune sera against SeseC_00396 could efficiently kill the bacteria. In addition, it was also found that …

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Prevalence And Risk Factors Of Haemotropic Mycoplasma Ovis Infection In Selected Smallholder Sheep And Goat Flocks In Malaysia, Bura Thlama Paul, Faez Firdaus Abdullah Jesse, Eric Lim Teik Chung, Azlan Che-Amat, Mohd Lila Mohd-Azmi Jun 2021

Prevalence And Risk Factors Of Haemotropic Mycoplasma Ovis Infection In Selected Smallholder Sheep And Goat Flocks In Malaysia, Bura Thlama Paul, Faez Firdaus Abdullah Jesse, Eric Lim Teik Chung, Azlan Che-Amat, Mohd Lila Mohd-Azmi

The Thai Journal of Veterinary Medicine

Outbreaks of haemotropic Mycoplasma ovis cause haemolytic anaemia with decreased production outcomes and mortality in sheep and goats worldwide but there is a lack of published data on the epidemiology of M. ovis in Malaysia's small ruminant flocks. This preliminary study investigates the prevalence and risk factors of Mycoplasma ovis infection in selected smallholder farms in Negeri Sembilan state. A total of 341 blood samples was randomly collected from 5 farms between January and December 2019. The farmers also completed a structured questionnaire to provide farm management data and environmental information. Giemsa stained blood smears were examined microscopically to detect …

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Immune-Responsiveness, Performance And Blood Chemistry Of Broiler Chickens Fed Black Cumin Seed And/Or Turmeric, Sabry M. El-Bahr, A. Al-Ankari, Saad Shousha Jun 2021

Immune-Responsiveness, Performance And Blood Chemistry Of Broiler Chickens Fed Black Cumin Seed And/Or Turmeric, Sabry M. El-Bahr, A. Al-Ankari, Saad Shousha

The Thai Journal of Veterinary Medicine

Two hundred and fifty one-day old broiler chicks were used to evaluate the effect of black seeds and/or turmeric on performance and immune response to Newcastle Disease Virus (NDV), Infectious Bronchitis Virus (IBV) and Infectious Bursal Disease virus (IBDV) in broiler chickens of six weeks of age. The birds were divided into five groups (50 birds in each) with 5 replicates (10 birds each). Birds in the first group were fed a basal diet and served as a control. Birds of groups 2-5 were fed a basal diet mixed with black seed (20g/ kg basal diet), turmeric (20g/ kg basal …

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The Effect Of Low-Level Laser Therapy On Rat Unicortical Femoral Defect, Latif Emrah Yanmaz, Ugur Ersoz, Sitkican Okur, Betul Apaydin Yildirim, Selim Comakli, Yavuz Selim Sağlam, Elif Dogan, Ozhan Karatas, Ferda Turgut, Zafer Okumuş Jun 2021

The Effect Of Low-Level Laser Therapy On Rat Unicortical Femoral Defect, Latif Emrah Yanmaz, Ugur Ersoz, Sitkican Okur, Betul Apaydin Yildirim, Selim Comakli, Yavuz Selim Sağlam, Elif Dogan, Ozhan Karatas, Ferda Turgut, Zafer Okumuş

The Thai Journal of Veterinary Medicine

The aim of this study was to determine the effectiveness of low-level laser therapy (LLLT) in the healing of experimentally created unicortical defect (UCD) of rat femurs. Twenty-four adult Wister rats were randomly divided into 2 groups: UCD and UCD+LLLT. The animals were subdivided into 3 groups (each group had 4 animals) and then they sacrificed at day 8, 15 and 22 for radiological, pathological and biochemical analysis. Bone healing was significantly different between the groups at day 15 and 22 (P < 0.05), while bone formation was significantly increased in the UCD+LLLT group at day 22 compared to the UCD group (P < 0.05). On histopathological examination, significant difference was found between the UCD and UCD+LLLT groups on the 22nd day (P < 0.05) while there was no difference on the 8th and 15th day. In the UCD group, the plasma Alkaline phosphatase activity decreased on the 15th and 22nd days, Calcium on the 15th day and Phosphorus on the 8th and 22nd days compared to the UCD+LLLT group. In addition, Glutathione levels, Plasma Catalase, Glutathione Peroxidase and Superoxide Dismutase activity significantly reduced in the UCD group compared with UCD+LLLT on the 8th, 15th and 22nd day (P < 0.05). In conclusion, LLLT applied at a dose of 904µm accelerates fracture healing in UCD of a rat’s femur. However, this effect shows an increase after the 22nd day of LLLT application.

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Occurrence And Sequence Type Of Antimicrobial Resistant Salmonella Spp. Circulating In Antibiotic-Free Organic Pig Farms Of Northern-Thailand, Pakpoom Tadee, Prapas Patchanee, Ben Pascoe, Samuel K. Sheppard, Dethaloun Meunsene, Tunyamai Buawiratlert, Phacharaporn Tadee Jun 2021

Occurrence And Sequence Type Of Antimicrobial Resistant Salmonella Spp. Circulating In Antibiotic-Free Organic Pig Farms Of Northern-Thailand, Pakpoom Tadee, Prapas Patchanee, Ben Pascoe, Samuel K. Sheppard, Dethaloun Meunsene, Tunyamai Buawiratlert, Phacharaporn Tadee

The Thai Journal of Veterinary Medicine

Salmonella is one of the major foodborne pathogens, often as the result of pork consumption. Currently, with the situation of antimicrobial resistance, organic farming has been suggested as an alternative for healthier options. However, there is little evidence to support this. In this study, we investigated the occurrence of Salmonella circulating in local organic pig farms in northern Thailand and typed isolated clones to better understand the population structure and transmission dynamics of the underlying Salmonella contamination. In total, 112 samples from 11 organic pig farms were processed from October to December 2018. Salmonella was detected in 9 targeted farms. …

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Possibility Of Trypsin Treated Dog Erythrocytes As Universal Blood: Evaluation By Ftir Microspectroscopy, Pornphimon Metheenukul, Kanjana Thumanu, Thanit Metheenukul Jun 2021

Possibility Of Trypsin Treated Dog Erythrocytes As Universal Blood: Evaluation By Ftir Microspectroscopy, Pornphimon Metheenukul, Kanjana Thumanu, Thanit Metheenukul

The Thai Journal of Veterinary Medicine

A common canine erythrocyte antigen is DEA 1.1 but it is the most immunogenic. In this study, Fourier transformed infrared (FTIR) microspectroscopy was used to examine the alterations of membrane biomolecules in trypsin-treated DEA 1.1+ canine erythrocytes. Production of canine DEA 1.1- erythrocytes was achieved by treating DEA 1.1+ red cells (5% cell suspension) with 1 mg/ml trypsin for 120 mins, without affecting the intracellular 2,3-diphosphoglycerate level. FTIR microspectroscopy was employed to study the effects of trypsin-treatment on canine trypsinized red cell membrane components. Production of canine DEA 1.1- erythrocytes was achieved without affecting the intracellular 2,3-diphosphoglycerate level. The FTIR …

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A Rapid Fecal Immunochemical Test For Gastrointestinal Bleeding Detection In Cynomolgus Macaques (Macaca Fascicularis), Taratorn Kemthong, Krittiga Sapkanarak, Piyachada Rounmakok, Thipchompoo Sing-Ayudthaya, Lalitta Suriya-Arunroj, Florian Föger, Martin Werle, Suchinda Malaivijitnond Jun 2021

A Rapid Fecal Immunochemical Test For Gastrointestinal Bleeding Detection In Cynomolgus Macaques (Macaca Fascicularis), Taratorn Kemthong, Krittiga Sapkanarak, Piyachada Rounmakok, Thipchompoo Sing-Ayudthaya, Lalitta Suriya-Arunroj, Florian Föger, Martin Werle, Suchinda Malaivijitnond

The Thai Journal of Veterinary Medicine

We used the Fecal Immunochemical Occult Blood Test (FIT) kit, a lateral flow immunochromatographic assay specific for human hemoglobin, on cynomolgus monkeys (Macaca fascicularis). We aimed to employ this test to detect gastrointestinal injury in captive animals at the National Primate Research Center of Thailand-Chulalongkorn University (NPRCT-CU). The cross-reactivity and sensitivity of the test for monkey blood were determined and compared with the human blood. The anti-human hemoglobin antibody of the FIT kit reacted with the monkey blood in a similar way as it did with humans and the intensity (T/C ratio) values between the two data sets closely correlated …

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Downregulation Of Kynurenine 3-Monooxygenase Inhibits Cancer Stemness-Related Genes In Both Human And Canine Breast Tumor Cell Lines, Chia-Tang Chen, Chiao-Hsu Ke, Hsin-Pei Weng, Chi-Hsun Liao, Chen-Si Lin Jun 2021

Downregulation Of Kynurenine 3-Monooxygenase Inhibits Cancer Stemness-Related Genes In Both Human And Canine Breast Tumor Cell Lines, Chia-Tang Chen, Chiao-Hsu Ke, Hsin-Pei Weng, Chi-Hsun Liao, Chen-Si Lin

The Thai Journal of Veterinary Medicine

Canine mammary tumors (CMT) and human breast cancer (HBC) are the most frequent cancers in both female dogs and women worldwide, and they share an urgency for the development of effective markers. Overexpression of kynurenine 3-monooxygenase (KMO), a secondary enzyme of the kynurenine pathway, has been shown to indicate poor prognosis in CMT patients. To further investigate the role of KMO in mammary tumor development, we first analyzed the significant pathways related to KMO from the Database for Annotation, Visualization and Integrated Discovery (DAVID). The results revealed that several Kyoto Encyclopedias of Genes and Genomes (KEGG) pathways might correlate with …

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The Use Of Human Suprapubic Bladder Catheter To Manage Obstructive Urolithiasis In A Saanen Buck, Nurul Hayah Khairuddin, Mark Wen Han Hiew, Syahirah Ahmad Affandi, Azim Salahudin Muhamad, Nur Asyikin Haron, Rozanaliza Radzi, Chen Hui Cheng, Ganesh Vythilingam Jun 2021

The Use Of Human Suprapubic Bladder Catheter To Manage Obstructive Urolithiasis In A Saanen Buck, Nurul Hayah Khairuddin, Mark Wen Han Hiew, Syahirah Ahmad Affandi, Azim Salahudin Muhamad, Nur Asyikin Haron, Rozanaliza Radzi, Chen Hui Cheng, Ganesh Vythilingam

The Thai Journal of Veterinary Medicine

A three-year-old Saanen buck presented with a complaint of stranguria for 24 hours. A physical examination revealed that the perineal urethra was turgid, swollen, and tender, and the urinary bladder was distended. The goat was diagnosed with obstructive urolithiasis. A percutaneous tube cystotomy technique was performed to relieve progressive urinary bladder distension. Using a human suprapubic urinary bladder catheter system, the technique was performed twice due to the recurrence of the blocked urethra. Following drainage of the urine, all vital parameters returned to normal. After surgical cystotomy, an intraoperative observation revealed the urinary bladder was filled with white sandy urolith, …

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Effect Of Daily Environment And Season In A Tropical Environment On The Expression Of Heat Shock Protein, And Steroidogenic And Apoptotic Genes In Bovine Cumulus-Oocyte Complex, S. Kanwichai, S. Panasophonkul, J. K. Bernard, W. Suriyasathaporn Jun 2021

Effect Of Daily Environment And Season In A Tropical Environment On The Expression Of Heat Shock Protein, And Steroidogenic And Apoptotic Genes In Bovine Cumulus-Oocyte Complex, S. Kanwichai, S. Panasophonkul, J. K. Bernard, W. Suriyasathaporn

The Thai Journal of Veterinary Medicine

The objectives of this study were to determine the differences in the expression of heat shock protein, and steroidogenic and apoptosis genes by cumulus-oocyte complex (COC) of beef cattle in a tropical environment as affected by seasonal and day effect of temperature and relative humidity. Real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to measure gene expression in COC in different seasons including cool (Jan to Feb), summer (Mar to Apr) and rainy (May to Jul) seasons. Ambient temperature and relative humidity recorded on the day before oocyte collection were used to classify environment groups as: low temperature and low …

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Boar Sperm Production In A Tropical Environment, Janyaporn Rungruangsak, Somchai Sangkaphet, Kakanang Buranaamnuay, Panupan Pongpeng, Padet Tummaruk Jun 2021

Boar Sperm Production In A Tropical Environment, Janyaporn Rungruangsak, Somchai Sangkaphet, Kakanang Buranaamnuay, Panupan Pongpeng, Padet Tummaruk

The Thai Journal of Veterinary Medicine

In a tropical environment, sow productivity is strongly associated with boar sperm production. Regular monitoring of semen quality in boars that produce semen for artificial insemination is important. The objective of the present study was to investigate the boar sperm production in an evaporative cooling system in a tropical environment in association with breed and season. A total of 2878 ejaculates from 68 boars were collected from a boar stud in the western region of Thailand. The breeds consisted of 22 Duroc, 22 Yorkshire, 18 Landrace and six hybrid boars. Seasons were classified as cool, hot or rainy. Semen volume, …

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Nutrient Digestibility, Fermentation Pattern, Blood Biochemical Level And Inflammatory Response Of Nulliparous Dairy Goats Fed With Various Levels Of Oil Palm Fronds, Ulia Renfelia Baysi, Pramote Paengkoum, Wanwisa Ngampongsai, Chaiyawan Wattanachant, Pin Chanjula, Pitunart Noosen Jun 2021

Nutrient Digestibility, Fermentation Pattern, Blood Biochemical Level And Inflammatory Response Of Nulliparous Dairy Goats Fed With Various Levels Of Oil Palm Fronds, Ulia Renfelia Baysi, Pramote Paengkoum, Wanwisa Ngampongsai, Chaiyawan Wattanachant, Pin Chanjula, Pitunart Noosen

The Thai Journal of Veterinary Medicine

This study was conducted to investigate the effect of oil palm frond (OPF) inclusion in total mixed ration (TMR) diets on nutrient digestibility, fermentation pattern, blood biochemical level and inflammatory response in nulliparous dairy goats. Three nulliparous female dairy goats (Saanen x Thai native, 33.88±1.78 kg) were randomly assigned in a 3x3 Latin square design. The diets consisted of 0% OPF (CON), 20% OPF (LOPF) and 40% OPF (HOPF) on a dry matter (DM) basis. The inclusion of OPF in the diets significantly increased the DM and nutrient intake of the dairy goats (p<0.05). There was no significant difference in the digestibility of DM, organic matter and crude protein of LOPF and HOPF, (p>0.05) but those were significantly higher …

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Multidrug Resistant Escherichia Coli Harboring Extendedspectrum Β-Lactamase-Encoding Genes Isolated From Clinically Healthy Pigs, Khin Khin Lay, Haidee E. Torio, Asinamai Athliamai Bitrus, Wanida Mala, Nuananong Sinwat, Rungtip Chuanchuen Jun 2021

Multidrug Resistant Escherichia Coli Harboring Extendedspectrum Β-Lactamase-Encoding Genes Isolated From Clinically Healthy Pigs, Khin Khin Lay, Haidee E. Torio, Asinamai Athliamai Bitrus, Wanida Mala, Nuananong Sinwat, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

A total of 292 E. coli isolates obtained from fecal samples from pigs in Central (n = 103) and Northeastern (n = 189) provinces of Thailand were included in this study. Eighty-six E. coli isolates were phenotypically confirmed to be β- lactamases producers and screened for the presence of β-lactamase genes. The CTX-M family was most frequently identified (90.7%). The blaCTX-M-15 gene belonging to CTX-M group I (59.3%) was the most predominantly identified CTX-M genotype followed by blaCTX-M-14 (31.4%) and blaCTX-M-4 (25.6%). The blaTEM-1 gene was prevalent (75.6%). The blaCTX-M-4 and blaCTX-M-14 genes were located on conjugative plasmid. The results …

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