Veterinary Medicine Commons^™

A Joint Matrix Completion And Filtering Model For Influenza Serological Data Integration., Xiao-Tong Yuan, Tong Zhang, Xiu-Feng Wan

CVM Publications

Antigenic characterization based on serological data, such as Hemagglutination Inhibition (HI) assay, is one of the routine procedures for influenza vaccine strain selection. In many cases, it would be impossible to measure all pairwise antigenic correlations between testing antigens and reference antisera in each individual experiment. Thus, we have to combine and integrate the HI tables from a number of individual experiments. Measurements from different experiments may be inconsistent due to different experimental conditions. Consequently we will observe a matrix with missing data and possibly inconsistent measurements. In this paper, we develop a new mathematical model, which we refer to …

Heterogeneous Nuclear Ribonucleoprotein K Supports Vesicular Stomatitis Virus Replication By Regulating Cell Survival And Cellular Gene Expression, Phat X. Dinh, Anshuman Das, Rodrigo Franco, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a member of the family of hnRNPs and was recently shown in a genome-wide small interfering RNA (siRNA) screen to support vesicular stomatitis virus (VSV) growth. To decipher the role of hnRNP K in VSV infection, we conducted studies which suggest that the protein is required for VSV spreading. Virus binding to cells, entry, and nucleocapsid uncoating steps were not adversely affected in the absence of hnRNP K, whereas viral genome transcription and replication were reduced slightly. These results indicate that hnRNP K is likely involved in virus assembly and/or release from …

Growing South Dakota (Summer 2013), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Fish, Field & Forest: Natural Resource Management Research Covers the Gamut
[Page] 5 Profiles In Leadership: David Willis; Combined Department Creates Interdisciplinary Opportunities
[Page] 6 Research On the Range: Cottonwood Field Station Has Provided Research Data for 106 Years
[Page] 8 Prairie Oasis: Natural Diversity Showcased at Oak Lake Field Station
[Page] 9 Special Section: Investigating in Agriculture & Biological Sciences at SDSU: An Update On the College of Ag’s Enhanced Facilities and Continuing Efforts Important to Helping Address Future Agricultural Challenges and Opportunities.[Page] 13 South Dakota 4-H Update: Skill-a-thons, New Exhibit Hall Planned
[Page] 14 After The …

Envelope Determinants Of Equine Lentiviral Vaccine Protection, Jodi K. Craigo, Corin Ezzelarab, Sheila J. Cook, Chong Liu, David W. Horohov, Charles J. Issel, Ronald C. Montelaro

Veterinary Science Faculty Publications

Lentiviral envelope (Env) antigenic variation and associated immune evasion present major obstacles to vaccine development. The concept that Env is a critical determinant for vaccine efficacy is well accepted, however defined correlates of protection associated with Env variation have yet to be determined. We reported an attenuated equine infectious anemia virus (EIAV) vaccine study that directly examined the effect of lentiviral Env sequence variation on vaccine efficacy. The study identified a significant, inverse, linear correlation between vaccine efficacy and increasing divergence of the challenge virus Env gp90 protein compared to the vaccine virus gp90. The report demonstrated approximately 100% protection …

Tricarboxylic Acid Cycle And One-Carbon Metabolism Pathways Are Important In Edwardsiella Ictaluri Virulence., Neeti Dahal, Hossam Abdelhamed, Jingjun Lu, Attila Karsi, Mark L. Lawrence

CVM Publications

Edwardsiella ictaluri is a Gram-negative facultative intracellular pathogen causing enteric septicemia of channel catfish (ESC). The disease causes considerable economic losses in the commercial catfish industry in the United States. Although antibiotics are used as feed additive, vaccination is a better alternative for prevention of the disease. Here we report the development and characterization of novel live attenuated E. ictaluri mutants. To accomplish this, several tricarboxylic acid cycle (sdhC, mdh, and frdA) and one-carbon metabolism genes (gcvP and glyA) were deleted in wild type E. ictaluri strain 93-146 by allelic exchange. Following bioluminescence tagging of the E. ictaluri ?sdhC, ?mdh, …

The 23rd Annual Research Conference Abstract Booklet, Mulu Aderie Alemu, Nikki Lynn Rogers

University of Gondar Research Conferences

Staff members, postgraduate and senior undergraduate students of the University, invited guests and speakers participated in the conference. The annual conference of the University is meant to share experiences in research activities among juniors and seniors, staff and students, and invited guests. It is also meant to motivate students and young faculty to engage in research and also to initiate and strengthen interdisciplinary collaborations. The findings of the studies and the resulting recommendations are expected to be used in solving the diverse societal problems we have been facing.

Research activities at the University of Gondar are primarily aimed at solving …

Glycerol Concentration Effects On Quality And Longevity Of Post-Thaw Goat Semen, Nitira Anakkul, Junpen Suwimonteerabutr, Theerawat Tharasanit, Nawapen Phutikanit, Jinda Singlor, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

This study determined the effects of glycerol concentration (7, 10 and 14% (v/v)) supplemented into freezing medium on post-thaw sperm qualities. Semen was collected from 3 Black Bengal bucks and then pooled to reduce individual variation. Motility, viability, morphology and acrosome integrity were evaluated before and after cryopreservation. Computer-assisted semen analysis was used to assess sperm motion patterns at 0, 1, 2 and 3 hours post-thawing. The laparoscopic artificial insemination was performed to test the fertilizing ability of frozen-thawed semen. The spermatozoa cryopreserved with 10% glycerol tended to have a higher percentage of motility, progressive motility, spermatozoa with intact acrosome …

Probable Pig To Duck Transmission Of The Pandemic H1n1 2009 (Ph1n1) And Its Reassortant In Commingling Experimental Condition, Nataya Charoenvisal, Juthatip Keawcharoen, Donruethai Sreta, Siriporn Tantawet, Suphattra Jittimanee, Jirapat Arunorat, Korakrit Poonsuk, Alongkorn Amonsin, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Pandemic H1N1 2009 (pH1N1) virus is considered as a low pathogenic influenza virus, however, it rapidly spread among humans and was finally found in the swine population of 6 continents. Interspecies transmissions among different animal species are of interest. In this study, sentinel ducks were commingled with pH1N1 or pandemic H1N1 reassortment virus (rH1N1) inoculated pigs in separate groups. According to the results, both studied viruses were able to cross-species transmit to a few sentinel ducks with mild or no clinical signs. Viral shedding measured by a modified real time RT-PCR detection from the oropharyngeal and cloacal swabs were also …

Preference Testing As Environmental Enrichment Assessment For Laboratory Mice, Navakanit Sachanonta, Waridtha Sa-Ngeunreung, Somchai Sa-Ing-Kaew, Raywadee Butraporn

The Thai Journal of Veterinary Medicine

In the field of biomedical research, a wide variety of environmental enrichment items are available for laboratory mice to fulfill its physiological and behavioral needs which influence the outcome of animal experiments. However, to take a decision on the most suitable enrichment, researchers are likely to consider the cost of enrichment item more than animal preference and animal well-being. To develop a suitable environmental enrichment program for mice in Laboratory Animal Center, National Institute of Health (LAC-Thai NIH), we evaluated mouse preference for cages containing different types of nesting material and enrichment device. We carried out a simple preference test …

Welfare Assessment Of Flight-Restrained Captive Birds: Effects Of Inhibition Of Locomotion, Shawn Jen-Lung Peng, Fang-Chia Chang, Judy Sheng-Ting I, Andrew Chang-Young Fei

The Thai Journal of Veterinary Medicine

Morphological and physiological adaptations have allowed birds to utilize flying as part of the primary locomotion. However, birds in captivity are often deprived of this natural skill due to limited living space or wing amputation. This article aims to investigate the essentialness of flight and welfare assessment of flight restraint in captive birds, using animal-based measurement. Ten wild-caught great mynahs (Acridotheres grandis) were chosen as test subject and evaluated in three separate studies. In pathology study, bird wings were partially amputated to assess possible neuroma formation at the site of amputation. The birds involved in this study were evaluated on …

Mutations In Topoisomerase Genes And Expression Of Acrab Multidrug Efflux System In Fluoroquinolone-Resistant Salmonella Enterica From Pork And Patients, Wechsiri Wannaprasat, Rungtip Chuanchuen1

The Thai Journal of Veterinary Medicine

The aim of this study was to further examine the mechanisms of ciprofloxacin resistance in Salmonella enterica isolates from pork and patients. Twenty-four isolates including 18 ciprofloxacin- resistant isolates and six susceptible strains were used. Mutations in the quinolone resistance-determining regions (QRDRs) of gyrA, gyrB, parC and parE were examined. Expression of AcrAB-TolC efflux pump was quantitatively determined using qRT-PCR. While nucleotide modifications were found in gyrA, gyrB and parC, all mutations identified were novel. All 18 ciprofloxacin resistant isolates carried mutations in at least one target gene and all six isolates susceptible to ciprofloxacin did not carry mutations in …

Surgical Removal Of Foreign Bodies In The Gastrointestinal Tract Of Monocellate Cobra, Naja Kaouthia, Taksa Vasaruchapong, Lawan Chanhome

The Thai Journal of Veterinary Medicine

A wild-captured female monocellate cobra (Naja kaouthia) had abnormal posture and move with difficulty as consequences of swelling at the middle third of the body. The swelling area was firm on palpation with average size of 10x4 cm. The shape of the swelling area was similar to a small bottle which was obviously recognized on the dorsal recumbency. Therefore, the foreign body obstruction in gastrointestinal tract was diagnosed without radiographic examination. Digital manipulation was performed, but could not move the foreign bodies. Therefore, gastrotomy was considered. The foreign bodies were a plastic bottle and a piece of cloth which were …

Comparison Of Repetitive Sequence-Based Polymerase Chain Reaction (Rep-Pcr) And Pulsed-Field Gel Electrophoresis (Pfge) For Genetic Characterization Of Arcobacter Spp., Panvipa Phasipol, Nipa Chokesajjawatee, Taradon Luangtongkum

The Thai Journal of Veterinary Medicine

Arcobacter has been associated with foodborne illness in humans. Recently, this organism has been receiving more attention as a pathogen of public health concern. The contamination of Arcobacter is frequently observed in foods of animal origin especially poultry products; however, the source of contamination as well as the molecular epidemiology of Arcobacter is not clearly understood. In the present study, we compared the use of repetitive sequence-based polymerase chain reaction (rep-PCR) and pulsed-field gel electrophoresis (PFGE) for genetic characterization of Arcobacter. Thirty Arcobacter butzleri isolates from retail chicken carcasses and 3 Arcobacter reference strains were typed with rep-PCR and PFGE. …

Susceptibility Of Clostridium Difficile Isolated From Healthy Captive Asian Elephants To Metronidazole And Vancomycin, Nattawooti Sthitmatee, Thanya Warinrak, Waroot Wongkalasin

The Thai Journal of Veterinary Medicine

Susceptibility to metronidazole and vancomycin, drugs of choice for Clostridium difficile infection, of 15 C. difficile isolates from 6 healthy Asian elephants was determined. All of the isolates belonged to only 1 ribotype pattern and carried both toxin A and B genes. The Minimal inhibitory concentration range of metronidazole and vancomycin, drugs of choice for treatment of C. difficile infection, was 0.125-4.0 μg/ml and 0.125-2.0 μg/ml, respectively. Moreover, MIC50 and MIC90 for metronidazole were 0.75 and 1.5 μg/ml while vancomycin was 1.0 and 2.0 μg/ml. There was no evidence of resistance to these antimicrobials. These results might be a preliminary …

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Mrna And Protein Expressions Of Prostaglandin E2 Receptors (Ep2 And Ep4), Cyclooxygenase-2 And Prostaglandin E Synthase In The Cervix Of Cyclic Bitches And Those With Pyometra, Pichanun Linharattanaruksa, Theerawat Swangchan-Uthai, Sayamon Srisuwatanasagul, Muhammad Khalid, Kaywalee Chatdarong

The Thai Journal of Veterinary Medicine

Prostaglandins play a vital role in regulation of cervical patency. Prostaglandin E2 (PGE2) synthesis is regulated by cyclooxygenase (COX) and prostaglandin E synthase (PGES). PGE2 acts through prostaglandin E receptors (EP), EP2 and EP4, to stimulate muscle relaxation. The aim of this study was to investigate mRNA expressions (EP2, EP4, COX-2, and PGES) and protein expressions (EP2, EP4, and COX-2) in the bitch cervix. Two groups of bitches, normal cyclic bitches and bitches with pyometra, were studied. Cyclic bitches were categorized into anestrus (n = 10), estrus (n = 7), and diestrus (n = 11), whereas bitches with pyometra were …

Coccidiosis In A Greater Slow Loris (Nycticebus Coucang), Wijit Banlunara, Sawang Kesdangsakonwut, Piyaporn Kongmekee, Angkana Sommanustweechai

The Thai Journal of Veterinary Medicine

Since Eimeria pachylepyron was firstly described in slow loris (Nycticebus coucang) in 1972, there has not been any reports of the disease throughout the world. An adult male 0.8 kg bw greater slow loris (Nycticebus coucang) had diarrhea, emaciation and dead. Severe necro-hemorrhagic jejunitis with the presence of coccidian protozoa and secondary bacterial septicemia were the causes of death. Endogenous stages of E. pachylepyron were detected exclusively in the jejunal enterocytes especially in the crypt of Lieberkühn. The typical round unsporulated oocysts with homogeneous eosinophilic thick wall were closely related to E. pachylepyron. This is the first report to confirm …

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

Indices Of Myocardial Contractility, Anusak Kijtawornrat

The Thai Journal of Veterinary Medicine

Myocardial contractility is defined as the velocity of cycling of heavy meromyosin cross-bridges. Contractility may be defined also as the velocity of myocardial fiber shortening or change in ventricular dimension during contraction when the fiber shortens or the ventricle contracts against no load. In the clinic; however, myocardial contractility may be estimated by some parameters of systolic function adjusted for the degree of stretch on the fibers (the preload) just before contraction begins and the hindrance to ejection of blood (the afterload). Excitation-contraction coupling is a physicochemical process during which electrophysiological changes (initiated by depolarization of the cell) translate to …

Expression Of Oestrogen Receptor Alpha And Progesterone Receptor In Sow Oviducts After Different Artificial Insemination, Sayamon Srisuwatanasagul, Padet Tummaruk, Annop Kunavongkrit

The Thai Journal of Veterinary Medicine

In pig production, different insemination methods which allow deposition of sperm in the uterine body (intrauterine insemination, IUI) and uterine horn (deep intrauterine insemination) have been applied in order to reduce number of spermatozoa. Earlier studies reported that steroid hormones influenced the transportation of spermatozoa, ovum and embryos in the sow reproductive tract which was related to the presence of their specific receptors; oestrogen receptor alpha (ERα) and progesterone receptor (PR). Therefore, the present study aims to evaluate the immunolocalization of ERα and PR in different parts of sow oviducts, after different artificial insemination methods. Twelve sows were divided into …

Effects Of Myogenic Factor 5 (Myf5) Gene On Carcass And Meat Quality Of Mong Cai Pigs, Thi Kim Khang Nguyen, Trong Ngu Nguyen

The Thai Journal of Veterinary Medicine

The aim of this study was to analyze the impact of Myogenic Factor 5 gene (MYF5) on carcass and meat quality of Mong Cai pigs. PCR-RFLP was applied to genotype 100 animals at three loci defined as MYF5/Hin1II, MYF5/HaeIII and MYF5/MspI. Results showed that MC pigs had two genotypes at each locus namely CD and DD,EF and EE, and GH and HH for the MYF5/Hin1II, MYF5/HaeIII and MYF5/MspI loci, respectively. The frequencies of DD, EE and HH were predominant (0.78-0.94) in the population. In addition, association analysis of three loci revealed that animals carrying CD genotype provided higher dressing percentage …

Development And Application Of Reverse Transcription Loop-Mediated Isothermal Amplification (Rt-Lamp) For Feline Coronavirus Detection, Somporn Techangamsuwan, Araya Radtanakatikanon, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Mutated feline coronavirus (FCoV) causes a highly fatal disease in cats, named feline infectious peritonitis (FIP). Common FIP clinical signs represent an accumulation of fluid in the thorax and/or abdomen. Screening tests currently rely on fluid analysis with cytologic examination to differentiate cellular components. Accordingly, accurate ante-mortem diagnosis is prerequisite for effective treatment strategies. This study focused on developing a rapid diagnostic tool for FCoV by using reverse transcription loop-mediated isothermal amplification (RT-LAMP). Clinical samples composed of bodily fluids, plasma and feces were obtained from FIP-suspected cats (n = 63), apparent healthy cats living with (n = 12) and without …

Effect Of Polysaccharide Gel Extracted From Durio Zibethinus Rind On Immune Responses, Bacteria Counts And Cholesterol Quantities In Chickens, Nustha Kitprathaung, Nattaya Ngamrojanavanich, Piyarat Chansiripornchai, Sununta Pongsamart, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

Polysaccharide gel (PG) from the rind of durian (Durio zibethinus Murr.) is a soluble powder that gives good results as an excipient in pharmacological and food preparation. The aim of the present study was to evaluate the effects of PG as a feed-supplement diet on body weight gain, immune stimulation, total bacteria and Salmonella in feces, and cholesterol levels in broilers. Eighty, one-day-old broiler chicks were divided into 4 groups. Three experimental groups were fed a commercial diet coated with PG 1, 2 and 3 g/100 g, respectively, and the control group was fed a commercial diet without PG. The …

Production Of Black Goat Using Laparoscopic Artificial Insemination And Embryo Transfer, Nitira Anakkul, Junpen Suwimonteerabutr, Theerawat Tharasanit, Saritvich Panyaboriban, Sarawanee Khunmanee, Natthakarn Thanomsuksinchai, Mongkol Techakumphu1

The Thai Journal of Veterinary Medicine

Laparoscopic artificial insemination (LAI) and embryo transfer (ET) were used to produce cross-bred blackcolored goat. In Experiment 1, LAI with frozen-thawed semen of black buck (Australian Melaan) was performed in 75% Saanen cross-bred does (white color, n = 70). The total numbers of 68 kids were born from 50 does. The skin colors of kids born were black (10.29%), white (39.71%) and other colors (50%). In Experiment 2, two cross-breeding programs were tested including program I: frozen semen of Australian Melaan inseminated to Black Bengal female (n = 7) and program II: frozen semen of Black Bengal inseminated to 50% …

Prevalence Of Salmonella Enterica, Escherichia Coli And Staphylococcus Aureus In Raw Meat In Thai Self-Service Style Restaurants In Khon Kaen Municipality, Sunpetch Angkititrakul, Arunee Polpakdee, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

One hundred and fifty eight raw meat samples were collected from 33 Thai self-service style restaurants in Khon Kaen municipality during July-October 2011. The fresh meat included pork, beef and chicken and was provided for self-grilling or boiling. All the samples were examined for contamination of three major foodborne pathogens including Salmonella enterica, Escherichia coli and Staphylococcus aureus using standard protocol. High prevalence of S. enterica (44%), E. coli (60%) and S. aureus (25%) was observed. Seventy-seven percent of the samples were positive for at least one of the three pathogens, while only 8% were found to carry all three …

Histological Characteristics Of Auditory Tube Diverticulum Of Domestic Donkey (Equus Asinus), Seyed Morteza Ghazi, Behzad Mobini

The Thai Journal of Veterinary Medicine

The purpose of this study was to investigate histological characteristics of auditory tube diverticulum in domestic donkeys (Equus asinus) using conventional histological and histochemical methods. Specimens were collected from twenty regions (five different locations of each compartment of auditory diverticulum). The mucosa consisted of pseudostratified columnar epithelium with brush-like cilia and forms of some non-isometric folds. The few goblet cells, which were irregularly distributed over the whole luminal surface, reacted strongly with Alcian blue pH 2.5, whereas neutral mucosubstances were not observed. The lamina muscularis mucosa was absent. The lamina propria-submucosa contained all connective tissue fibers, tubulo-acinar glands, parasympathetic ganglia, …

Antiparasitic Efficacy Of 10% W/V Fipronil Spot-On (Fiproline Spot-On) Against Experimental Tick (Rhipicephalus Sanguineus) Infestations On Dogs, Sonthaya Tiawsirisup, Kanokwan Thiansirikhun, Kanoksak Thanadumkerng, Nuttika Pastarapatee, Nunaree Trirattananuwong, Wilai Rattanatayarom

The Thai Journal of Veterinary Medicine

This study was conducted to investigate the efficacy of 10% w/v fipronil spot-on for treatment and prevention of Rhipicephalus sanguineus infestations on dogs. Twelve Beagles were randomly separated into two groups which were treatment and control group (6 dogs/group). The treatment group received 10% fipronil spot-on at the dosage of 6.7 mg/kg on day 0. A group of 60 Rhipicephalus sanguineus was released to feed on each dog on day -7, -2, 7, 14, 21, and 28. The ticks were counted and removed on days-4, 3, 10, 17, 24, and 31. Mean number of tick infestations in the control group …

Different Influence Of Intracellular Glutamine Depletion On Glutamate Uptake Mediated By Amino Acid Transport Systems; Eaats And X-C, In Placental (Bewo) Cells, Boonrit Thongsong

The Thai Journal of Veterinary Medicine

Glutamate transporters are responsible for active transport of glutamate over the cell membrane and play an important role in the glutamate-glutamine cycle. The purpose of the present study was to investigate the influence of intracellular glutamine depletion on glutamate uptake mediated by amino acid transport systems; EAATs and X-c in placental choriocarcinoma (BeWo) cells as a model of human trophoblasts. Intracellular glutamine was depleted by culturing the cells in regular medium without glutamine and by treating with an inhibitor of glutamine synthetase (methionine sulfoximine; MSX), for 16 hours. The uptake of glutamate was measured by the use of appropriate substrates …

Genetic Diversity Of Spirometra Erinaceieuropaei From Dogs In Hunan Province, China Based On Analyses Of Two Mitochondrial Sequences, Liang Liang, Jian Liang, Hui Shen, Xiao Lei Yao

The Thai Journal of Veterinary Medicine

Sequence variability in two mitochondrial DNA (mtDNA) regions, namely NADH dehydrogenase subunit 5 (nad5) and small subunit of ribosomal RNA (rrnS) in Spirometra erinaceieuropaei from dogs in Hunan province in China was examined. A portion of the nad5 (pnad5) and rrnS (prrnS) genes were amplified separately from individual S. erinaceieuropaei by polymerase chain reaction (PCR). Representative amplicons were subjected to sequencing in order to estimate sequence variability. The sequences of pnad5 and prrnS were 531 and 328 bp in size, respectively. The intra-specific sequence variations within each of the S. erinaceieuropaei were 0-3.5% for pnad5 and 0-1.4% for prrnS, while …

Implications Of Cat Ownership Statistics And Social Changes: A Longitudinal Study In Taiwan From 2001 To 2009, Li Huan Chen, Ting Wei Chang, Meng Chih Tung, Chang Young Fei, I Tsun Chiang

The Thai Journal of Veterinary Medicine

Pet ownership statistics in Taiwan had not been investigated until Animal Protection Act was announced on November 4, 1998. This longitudinal study aims to investigate the demographic information of household cats in Taiwan and discuss its implications with social changes from 2001 to 2009 biennially. Household cats were surveyed biennially by randomly dialed telephone survey. A structured questionnaire was designed to obtain household demographics and population-associated data on pet ownership. Related social changes data were obtained from the Statistical Yearbook of the Ministry of Interior, Taiwan. Results showed that household cat ownership increased from 2001 to 2009, but the average …