Veterinary Medicine Commons^™

Katrina's Animal Legacy: The Pets Act, Marita Mike, Rebecca Mike, Clark J. Lee

Homeland Security Publications

This article discusses issues related to the federal Pets Evacuation and Transportation Standards Act of 2006 (PETS Act), which was signed into law in the aftermath of Hurricane Katrina. Issues discussed in this article include:

1. Various problems concerning animal evacuations and sheltering that Hurricane Katrina brought to light;
2. Provisions of the PETS Act and related laws and policies which were developed in response to the tragedies brought about by Hurricane Katrina; and
3. Strengths and weaknesses of the PETS Act and recommends next steps to improve implementation of the PETS Act.

Consensus-Based Reporting Standards For Diagnostic Test Accuracy Studies For Paratuberculosis In Ruminants, Ian A. Gardner, Søren S. Nielsen, Richard J. Whittington, Michael T. Collins, Douwe Bakker, Beth Harris, Srinand Sreevatsan, Jason E. Lombard, Raymond Sweeney, David L. Smith, Jerie Gavalchin, Shigetoshi Eda

Zoonotics and Wildlife Disease: Publications

The Standards for Reporting of Diagnostic Accuracy (STARD) statement was developed to encourage complete and transparent reporting of key elements of test accuracy studies in human medicine. The statement was motivated by widespread evidence of bias in test accuracy studies and the finding that incomplete or absent reporting of items in the STARD checklist was associated with overly optimistic estimates of test performance characteristics. Although STARD principles apply broadly, specific guidelines do not exist to account for unique considerations in livestock studies such as herd tests, potential use of experimental challenge studies, a more diverse group of testing purposes and …

Bovine Tuberculosis In A Nebraska Herd Of Farmed Elk And Fallow Deer: A Failure Of The Tuberculin Skin Test And Opportunities For Serodiagnosis, W. Ray Waters, Gary E. Stevens, Mark A. Schoenbaum, Kathy A. Orloski, Suelee Robbe-Austerman, N. Beth Harris, S. Mark Hall, Bruce V. Thomsen, Arach J. Wilson, Roger E. Brannian, Jeffrey T. Nelson, Shawn Schafer, Javan Esfandiari, Meghan Dutton, Rena Greenwald, Konstantin P. Lyashchenko

Zoonotics and Wildlife Disease: Publications

In 2009, Mycobacterium bovis infection was detected in a herd of 60 elk (Cervus elaphus) and 50 fallow deer (Dama dama) in Nebraska, USA. Upon depopulation of the herd, the prevalence of bovine tuberculosis (TB) was estimated at ∼71–75%, based upon histopathology and culture results. Particularly with elk, gross lesions were often severe and extensive. One year ago, the majority of the elk had been tested for TB by single cervical test (SCT), and all were negative. After initial detection of a tuberculous elk in this herd, 42 of the 59 elk were tested by SCT. …

Distribution Of Culicoides Sonorensis (Diptera: Ceratopogonidae) In Nebraska, South Dakota, And North Dakota: Clarifying The Epidemiology Of Bluetongue Disease In The Northern Great Plains Region Of The United States, E. T. Schmidtmann, M. V. Herrero, A. L. Green, D. A. Dargatz, J. M. Rodriquez, T. E. Walton

Zoonotics and Wildlife Disease: Publications

The presence or absence of the biting midge Culicoides sonorensis Wirth & Jones (Diptera: Ceratopogonidae), a primary vector of bluetongue viruses (genus Orbivirus, family Reoviridae, BTV) in North America, was assessed on ranches and farms across the Northern Great Plains region of the United States, specifically Nebraska, South Dakota, and North Dakota, as part of a 2-yr regional study of BTV exposure among cattle. Blacklight/suction trap samples totaling 280 2-night intervals were taken at 140 aquatic sites (potential larval habitat for C. sonorensis) on 82 livestock operations (ranches and farms) that span a south-to-north gradient of expected decreasing …

Effect Of Ejaculation Frequency On Spermatozoa Survival In Diluted Semen From Pleven Blackhead Rams, Stanimir Yotov, Ivan Fasulkov, Nasko Vassilev

Turkish Journal of Veterinary & Animal Sciences

The effect of ejaculation frequency on spermatozoa survival in diluted Pleven Blackhead ram semen was investigated. Thirty-two ejaculates were examined. From each ram 4 ejaculates were collected at 10-min intervals in the morning and in the afternoon. Semen parameters, namely, color, density, transparency, volume, concentration, motility, and abnormal sperm, for each ejaculate were determined. The sperm motility was assessed from the moment of dilution up to 60 min at 10-min intervals, then again at 90 min and 120 min. The semen characteristics were determined by means of "Motic Image Plus" equipment and the data were processed statistically. Sperm volume and …

2011 Nebraska Beef Cattle Report

Nebraska Beef Cattle Reports

Cow/Calf

• Winter Grazing System and Supplementation of Beef Cows During Late Gestation Influence Heifer Progeny
• Influencing Steer Performance Through Maternal Nutrition
• Integration of DNA Marker Information into Breeding Value Predictions
• Granulosa Cell Gene Expression is Altered in Follicles from Cows with Differing Reproductive Longevity

Growing

• Performance of Growing Cattle Fed Corn Silage or Grazing Corn Residue from Second Generation Insect-protected (MON 89034), Parental, or Reference Corn Hybrids
• Nutrient Composition of Spoiled Wet Byproducts Mixed and Stored with Straw
• Use of Dry Rolled Corn, Dry or Wet distillers Grains Plus Solubles as an Energy Source in High Forage Diets for Growing …

Roles Of The 15-Kda Selenoprotein (Sep15) In Redox Homeostasis And Cataract Development Revealed By The Analysis Of Sep 15 Knockout Mice, Marina V. Kasaikina, Dmitri E. Fomenko, Vyacheslav M. Labunskyy, Salil A. Lachke, Wenya Qiu, Juliet A. Moncaster, Jie Zhang, Mark W. Wojnarowicz, Sathish Kumar Natarajan, Mikalai I. Malinouski, Ulrich Schweizer, Petra A. Tsuji, Bradley A. Carlson, Richard L. Maas, Marjorie F. Lou, Lee E. Goldstein, Dolph L. Hatfield, Vadim N. Gladyshev

School of Veterinary and Biomedical Sciences: Faculty Publications

The 15-kDa selenoprotein (Sep15) is a thioredoxin-like, endoplasmic reticulum-resident protein involved in the quality control of glycoprotein folding through its interaction with UDP-glucose:glycoprotein glucosyltransferase. Expression of Sep15 is regulated by dietary selenium and the unfolded protein response, but its specific function is not known. In this study, we developed and characterized Sep15 KO mice by targeted removal of exon 2 of the Sep15 gene coding for the cysteinerich UDP-glucose:glycoprotein glucosyltransferase-binding domain. These KO mice synthesized a mutant mRNA, but the shortened protein product could be detected neither in tissues nor in Sep15 KO embryonic fibroblasts. Sep15 KO mice were viable …

The Impact Of Tricaine Methanesulfonate, 2-Phenoxyethanol, And Carvone-Methyl Salicylate On The Innate Immune Response Of Zebrafish (Danio Rerio), Charles R. Wulff

Honors Theses

Anesthesia plays a vital role in the maintenance of aquaculture species, where it is used to minimize stress during complex handling tasks such as transport, assessment, and harvesting. However, anesthetics have been shown to suppress the innate immune response, which could impact immunity and increase risk of infection. Tricaine methanesulfonate (MS-222) and 2-Phenoxyethanol (2-PE) represent two of the most commonly used anesthetics in aquaculture, with R-(+)-carvone, in the form of carvone-methyl salicylate (CMS) has recently been proposed as an alternative anesthetic for food fish. These three anesthetics were used to assess the influence of anesthetics on the immune system of …

Evaluation Of Mass Spectrometric Methods For Detection Of The Anti-Protozoal Drug Imidocarb, A. F. Lehner, J. A. Hitron, J. May, C. Hughes, R. Eisenberg, N. Schwint, D. P. Knowles, P. Timoney, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Imidocarb [N,N'-bis[3-(4,5-dihydro-1H-imidazol-2-yl)phenyl]urea, C₁₉H₂₀N₆O₁, m.w. 348.41] is a symmetrical carbanilide derivative used to treat disease caused by protozoans of the Babesia genus. Imidocarb, however, is also considered capable of suppressing Babesia-specific immune responses, allowing Babesia-positive horses to pass a complement fixation test (CFT) without eliminating the infection. This scenario could enable Babesia-infected horses to pass CFT-based importation tests. It is imperative to unequivocally identify and quantify equine tissue residues of imidocarb by mass spectrometry to address this issue. As a pretext to development of sensitive tissue assays, we have investigated possibilities of mass …

Histological Examination Of Testicular Cell Development And Apoptosis In The Ostrich Chick, Wei Lan, Ke-Mei Peng, Huazhen Liu, Hui Song, Yang Wang, Li Tang

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to observe the microstructure and ultrastructure of the testis, demonstrate testicular cell development and apoptosis, and elucidate regularity of development and apoptosis in ostrich chick testes. By employing light microscopy 3 obvious development characteristics were detected with ostrich age increasing: first, many primordial germ cells and a few spermatogonia were found while seminiferous tubule integrity was not evident in testes of 1-day-old ostrich chicks nor were primordial germ cells. In the 30-day-old ostrich chicks spermatogonia were completely differentiated, but very few primary spermatocytes were observed in testes at 45 days of age. Second, the …

Effects Of Varying Levels Of Protected Fat On Performance Of Shami Goats During Early And Mid Lactation, Hosam Titi

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the responses of early lactating Shami goats to varying amounts of dietary supplementation of Ca salts of fatty acids. Shami goats (n = 75) in their first week of lactation were randomly assigned to 3 isonitrogenous dietary (TMR) treatments containing 0%, 3%, or 5% added Ca salt. The study took 60 days during which the following variables were measured: milk production and composition, body weight, feed intake, serum cholesterol and triglyceride, kids weaning weight, and milk fatty acids. Milk production and energy corrected milk were higher (P < 0.05) at 5% level. Percentages of milk composition were higher (P < 0.05) at 3% with CP% not different from 5%. No differences were observed in yields of different milk components including casein, final body weights, feed intake, or weaning weights of kids. Intake of metabolizable energy was higher (P < 0.05) at 5% level of Ca salts. Differences in milk fatty acids were mainly restricted to unsaturated ones with no effect on the saturated fatty acids. Such results suggest that milk production and content of lactating Shami goat can be changed by a level of 5% of Ca salts.

Improvement Studies On Mutton Sheep For Marmara Region Conditions: I. Fertility, Lamb Survival, And Growth Traits Of Lambs, Ayhan Ceyhan, Tamer Sezenler, İsmai̇l Erdoğan, Osman Torun

Turkish Journal of Veterinary & Animal Sciences

The present study was conducted to compare the fertility of ewes, the survival rates, and the growth performance of lambs that were crossbred by mating German Black-Headed Mutton (GBM) rams with Kıvırcık ewes. The average lambing rates of the GBM × K(F1), Bandırma-I and Bandırma-II genotype, and Kıvırcık ewes were 80.00%, 75.73%, 76.78%, and 83.72%, respectively. The differences in lambing rates between the genotypes were significant (P < 0.01). The genotypes had a significant effect on litter size at birth, but not on fecundity. The differences in lambs' survival rates between the genotypes were significant (P < 0.05). The birth weight (BW), weaning weight (WW), and yearling live weight (YLW) of the lambs were 3.77 kg, 34.11 kg, and 43.71 kg for the GBM × K (F1); 3.74 kg, 32.98 kg, and 44.91 kg for the Bandırma-I; 3.73 kg, 33.18 kg, and 45.71 kg for the Bandırma-II; and 3.45 kg, 30.92 kg, and 42.22 kg for the Kıvırcık lambs. The results of this study showed that crossbreeding between the GBM and Kıvırcık breed did not significantly increase lamb production. However, crossbred lambs did not have significant survival problems when compared to native Kıvırcık lambs and had higher growth performance than purebred Kıvırcık lambs.

The Increase Of Cd4+Cd25+ T Cells In The Peripheral Blood Of Pigs Persistently Infected With Porcine Reproductive And Respiratory Syndrome Virus, Jingwen Zeng, Danting He, Xin Guo, Xinna Ge, Hanchun Yang

Turkish Journal of Veterinary & Animal Sciences

Regulatory T lymphocytes (T-regs) may be involved in suppressing the host's immunity, which is important for viral clearance, and may therefore contribute to persistent viral infection. However, nothing is known regarding the situation of porcine T-regs during persistent infection with porcine reproductive and respiratory syndrome virus (PRRSV). This study, which used flow cytometry and real-time PCR, determined that the frequencies of CD4+CD25+ T-cells and porcine Forkhead/winged helix transcription factor (FoxP3) mRNA levels in peripheral blood significantly increased during PRRSV persistence, indicating that CD4+CD25+ T-regs might play an important role in PRRSV persistent infection. This study contributes to a better understanding …

The Effect Of Year And Season Of Birth, Sex, Sire, And Breeder On Ossification Of The Distal Epiphyseal Cartilage Of The Radial Bone In Thoroughbred Horses, Jaroslaw Luszczynski, Magdalena Pieszka

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the effect of specific factors on ossification of the distal epiphyseal cartilage of the radial bone in Thoroughbred horses. The study was carried out on 452 Thoroughbred horses, two years old, that were under race training. X-ray tests were conducted and interpreted every month starting at the 18th month of life and continuing until ossification of the distal epiphyseal cartilage of the radial bone occurred. In the statistical model, the year of birth, sex, birth season, sire, and breeder were treated as constant effects. Horses that were born in the spring season …

Double-Layer Mesh Hernioplasty For Repairing Umbilical Hernias In 10 Goats*, Jose M. Vilar, Juan A. Corbera, Giuseppe Spinella

Turkish Journal of Veterinary & Animal Sciences

Umbilical hernias in goats are uncommon and can vary in their etiology and management. Hernioplasty can be done by closing the abdominal wall with a horizontal mattress pattern using absorbable sutures. However, larger defects (hernial ring size >3 cm) generally require the use of prosthetic materials that allow for a tension-free repair. In this study, 10 young female goats with umbilical hernias and hernial ring sizes ranging from 7-10 cm in width were treated using a double-layer polypropylene mesh. An ultrasonographic examination was performed to assess healing and connective tissue production.

Genetic Immunization In The Horse: The Potential For Enhanced Immune Responses With Deacylated Polyethyleneimine (Pei) And Immunostimulatory Cytokines As Vaccine Adjuvants, Deborah Lee Even

Theses and Dissertations--Veterinary Science

DNA vaccines in larger animals, such as horses, are generally less effective and elicit significantly weaker immune responses, than in small animal model systems. To provide optimal protection against pathogenic microorganisms, the induction of both humoral and cellular immune responses from DNA vaccination may be necessary. One limitation to DNA immunization in the horse is the difficulty in generating high levels of antigen-specific antibody and CTL responses. Previous work in the laboratory has demonstrated that expression constructs containing native sequences encoding the surface unit (SU) envelope glycoprotein (pCiSU) of the Equine Infectious Anemia Virus (EIAV) are ineffective at stimulating immune …

Characterization Of Spiroplasma Mirum And Its Role In Transmissible Spongiform Encephalopathies, Hilari Maree French

LSU Doctoral Dissertations

There are many contradictory reports in the literature involving the culture of Spiroplasma mirum, its resistance to disinfectants and antibiotics, and its potential role in Transmissible Spongiform Encephalopathies (TSEs). These contradictions led to an interest in a possible link between spiroplasma and TSEs and the development of a research plan to elucidate this connection. It was hypothesized that Spiroplasma was associated with a neurodegenerative disease such as TSE. In this work we further characterized S. mirum laboratory strains and continued to evaluate the possible correlations they have with TSE infections and the prion. To optimize recovery of S. mirum from …

Aflatoxin M1 Contamination In Cow’S Milk In Kayseri (Central Turkey), Hi̇kmet Mavuş Buldu, A. Nedret Koç, Güven Uraz

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to determine the levels of aflatoxin M1 (AFM1) in the milk of cows in the villages of Kayseri province, Turkey. Ninety milk samples were investigated for AFM1 contamination using the enzyme linked immunoassay (ELISA). The mean value of AFM1 levels was 59.9 ng/L, 95% confidence interval was 54.4-65.5 ng/L, and standard deviation was 26.6 ng/L. For 63 samples (70%) the values were significantly above the limit permitted by European Union criteria (EU) and the Turkish Food Codex (TFC) (P < 0.05). However, the differences in AFM1 levels between villages were not significant (P > 0.05). In conclusion, since the level of AFM1 in the milk of cows was higher …

Eosinophils In Lung Tissue In 2 Chilean Criollo Horses With Exacerbated Recurrent Airway Obstructions “Heaves”, Alexander Ortloff, Hugo Folch, Gabriel Moran

Turkish Journal of Veterinary & Animal Sciences

We report on 2 cases of recurrent airway obstruction (RAO) observed in Chilean Criollo horses aged 13 and 15 years. Both animals were classified as having severe RAO. The analysis of the recovered cells of bronchoalveolar lavage fluid (BALF) showed high percentages of eosinophils (82% and 80% in the differential cell counts of the 2 horses) and poor responses to bronchodilator and corticosteroid drugs. The histopathological lung evaluation showed eosinophilic infiltration in the peribronchial tissue, as well as in the lumen of the bronchi and bronchioli. In both horses, immunohistochemistry confirmed high p65 homodimer activity in bronchial cells. This report …

Bilateral Testicular Hypoplasia Among Mature Sahel Bucks In Nigeria, Ikechukwu Igbokwe, Augustine Ikpo, Hafsat Grema, Fakilahyel Mshelbwala, Nanacha Igbokwe

Turkish Journal of Veterinary & Animal Sciences

Among 1608 Sahel goat bucks presented for slaughter at the Maiduguri Metropolitan abattoir in March-April 2007, 65.7% had plain white or black-white coat colour, and 91.2% were aged >1.5-3.0 years with mean body weights of 15.6-25.7 kg. Twenty-two (1.4%) of the mature bucks, aged 1.9 ± 0.3 years and weighing 20.6 ± 3.5 kg, had bilateral small hypoplastic testes that were symmetric, approximately 7 times smaller in weight, with a 2-fold reduction in longitudinal length and mid-circumference than the full-sized normal testes. The epididymes were also reduced in size as the testes. Histologically, the seminiferous tubules and caudal epididymides showed …

Statistical Evaluation Of Some Environmental Factors Affecting The Gross Efficiency Of Crude Protein In High Producing Dairy, Pouya Zamani, Seyed Reza Miraei Ashtiani, Daryoush Alipour, Hasan Aliarabi, Ali Asghar Saki, Ahmad Ahmadi, Khalil Zaboli

Turkish Journal of Veterinary & Animal Sciences

Some environmental factors affecting the gross efficiency of crude protein (GECP) were studied using 2824 records of 501 lactating Holstein cows. The effects of some independent variables on the GECP were tested using a general linear model analysis. The GECP was significantly affected by parity and pregnancy stage and linearly related to dry matter intake, milk yield, milk fat, protein and lactose percentages, protein and lactose yields, dietary levels of net energy for lactation (NEL), crude protein (CP), ruminally undegradable protein (RUP), and ether extract (EE). Quadratic relation to the GECP with dietary levels of NEL, CP, RUP, and EE …

Analysis Of Buffalo Liver Samples For The Presence Of Chlorpyrifos Residues By Using High Performance Liquid Chromatography, Pradeep Kumar, S.P. Singh, K. Shrikant, D. Madhukar

Turkish Journal of Veterinary & Animal Sciences

Food safety is of great importance to all involved in the food chain. The problem of pesticide residues is one of the concerns for the consumers. In the present study, 254 buffalo liver tissue samples were collected from 4 slaughterhouses and analyzed both qualitatively and quantitatively for the presence of chlorpyrifos residues. The samples were processed by extraction with acetonitrile followed by sonication and liquid-liquid partition. The extracts were cleaned up by performing alumina column chromatography. Chlorpyrifos residues in the samples were detected and quantified by employing high performance liquid chromatography. High performance liquid chromatography (HPLC) was performed by using …

Expression And Detection Of The Fmdv Vp1 Transgene And Expressed Structural Protein In Arabidopsis Thaliana, Li Pan, Yongguang Zhang, Yonglu Wang, Jianliang Lv, Peng Zhou, Zhongwang Zhang, Yuzhen Fang, Shoutian Jiang

Turkish Journal of Veterinary & Animal Sciences

To explore the feasibility of developing a new type of plantderived foot-and-mouth disease virus (FMDV) oral vaccine, the plant seed-specific expression vector p7SBin438/VP1 carrying the VP1 gene of the FMDV strain O/China/99 was constructed and transformed into Agrobacterium tumefaciens strain GV3101. This strain was used for transformation of Arabidopsis thaliana via the floral-dip method. The kanamycin-resistant transgenic plants were selected, and the VP1 gene and protein expressions were confirmed by PCR and sandwich-ELISA, respectively. The ELISA positive plants were further confirmed by Western blot assay, and the VP1 gene in the transgenic progeny was confirmed by PCR. The results showed …

Pathological And Immunohistochemical Evaluation Of The Effects Of Interferon Gamma (Ifn-Γ) And Aminoguanidine In Rats Experimentally Infected With Fasciola Hepatica, Enver Beytut, Ati̇la Akça, Hali̇l İbrahi̇m Gökce

Turkish Journal of Veterinary & Animal Sciences

The present study was aimed at pathologically and immunohistochemically evaluating the effects of interferon gamma (IFN-\gamma) and aminoguanidine (AG) in rats experimentally infected with Fasciola hepatica. A total of 44 Wistar rats were divided into 4 groups. With the exception of the controls (fourth group), the remaining rats were infected orally with 25 metacerceria of F. hepatica. The first group was administered 0.2% AG in drinking water daily, in order to block nitric oxide (NO) production. The second group was administered 250 units of IFN-\gamma daily, in order to stimulate NO synthesis. The third group was administered a placebo only. …

Effect Of Dietary Supplementation Of Mannan Oligosaccharides On Hydrogen Ion Concentration Of The Digestive Tract And Microbial Populations Of The Ceca Of Japanese Quail (Coturnix Japonica), Eleftherios Bonos, Efterpi Christaki, Amin Abrahim, Nikolaos Soultos, Panagiota Florou-Paneri

Turkish Journal of Veterinary & Animal Sciences

The effect of the dietary supplementation of mannan oligosaccharides (MOS) on hydrogen ion concentration of the digestive tract and microbial populations of the ceca of the Japanese quail (Coturnix japonica) was investigated in this experimentation. Two hundred twenty-five 1-day old Japanese quail divided into 3 groups, with 3 subgroups each, were fed a basal diet that served as control, or a basal diet containing 1 g MOS/kg or 2 g MOS/kg. On days 21 and 42, hydrogen ion concentrations of the quail crop, proventriculus, gizzard, duodenum, jejunum, ileum, large intestine, and ceca were measured in situ. Furthermore, on the same …

Infectious Pancreatic Necrosis In A Recirculating Rainbow Trout (Oncorhynchus Mykiss) Culture System, Nasrollah Ahmadi, Mostafa Akhlaghi, Ahmad Oryan, Arsalan Hosseini

Turkish Journal of Veterinary & Animal Sciences

During October 2009, a suspected outbreak of infectious pancreatic necrosis, with typical signs and high mortality, was observed in cultured rainbow trout (Oncorhynchus mykiss) fry (mean weight of 9 g) in a recirculating system in Fars Province, Iran. Tissue samples, including pyloric caeca as well as pancreatic tissues, liver, kidney, and spleen, were aseptically collected from 20 affected moribund fish for histopathological and molecular assays. Histopathologically, the exocrine pancreatic acinar cells exhibited the most notable pathology, including focal to extensive degeneration and coagulative necrosis with mononuclear inflammatory infiltrates. To confirm the diagnosis of clinical infectious pancreatic necrosis (IPN), a reverse …

First Report Of Fasciola Hepatica In Equus Caballus Host Species From Tunisia Based On The Ribosomal Internal Transcribed Spacer Regions, Nabil Amor, Sarra Farjallah, Khaled Said, Badreddine Ben Slimane

Turkish Journal of Veterinary & Animal Sciences

Fasciolosis caused by Fasciola spp. (Platyhelminthes: Trematoda: Digenea) is considered the most important helminth infection of ruminants in tropical countries, causing considerable socioeconomic problems. Samples identified morphologically as Fasciola sp. in Equus caballus host species from the city of Tunis (north of Tunisia) were genetically characterized, for the first time, by sequences of the 1st (ITS-1), the 5.8S and 2nd (ITS-2) Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA). Comparison of the ITS of the Tunisian samples with sequences of Fasciola spp. from GenBank showed that the specimens examined had sequences identical to those of F. hepatica sequences selected …

Chronic Continuous Perfusion Of The Hibernation Triggering Opioid Dadle Did Not Cause Any Major Physiological Alterations In Rats, İbrahi̇m Akyazi, Ülker Çöteli̇oğlu

Turkish Journal of Veterinary & Animal Sciences

[D-Ala2, D-Leu5] enkephalin (DADLE), a delta opioid receptor agonist, can induce mammalian hibernation and has hibernation-like effects in non-hibernating species. It has also been demonstrated that DADLE has protective efficacy on various tissues such as nervous, cardiac, and hepatic tissues. Despite substantial research conducted in the last decade about the tissue protective efficacy of DADLE, detailed underlying mechanisms have not been fully elucidated. In the present study we investigated in a rat model whether continuous perfusion of DADLE can induce potentially tissue protective alterations in physiological parameters. In the study 48 young male Wistar rats were used. They were divided …

Can The Vascular Endothelial Growth Factor (Vegf) And Total Estradiol 17Β Be Used As A Marker Of Equine Oocyte Maturation?, Mehmet Can Gündüz, Güven Kaşikçi, Özge Yilmaz, Si̇nem Özlem Engi̇nler, Meli̇h Uçmak, Ati̇la Ateş, Feraye Esen Gürsel, Gamze Evkuran, Meri̇h Duygu Töre

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to quantitate vascular endothelial growth factor (VEGF) and estradiol-17\beta in follicular fluid (FF) and serum and to correlate the levels of these substances with oocyte maturation. Transvaginal ultrasound-guided follicular aspirations were performed with the purpose of oocyte collection when the follicle was >35 mm in diameter. The mares were divided into 2 groups according to age, and the changes in VEGF and estradiol-17\beta in follicular fluid and serum were examined. Levels of estradiol-17\beta in the follicular fluid and serum of young mares (4-10 years old) did not differ from those in old mares (20-24 …

The Effect Of Organic Acid, Zeolite, Or Their Combination On Performance, Some Serum Indices, And Ileum Ph Values In Broilers Fed With Different Phosphorus Levels, İsmai̇l Abaş, Tanay Bi̇lal, Hüseyi̇n Eseceli̇

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the effects of the addition of organic acid (OA), zeolite (ZE), or both to broiler diets with microbial phytase containing low and adequate levels of phosphorus (P) on performance, some serum indices, and ileum pH values. For the study, 480 broiler chicks 1-day old were assigned equally to 8 groups, with 6 replicates (10 chicks per replicate) carried out over 42 days. A basal diet containing 600 FTU of phytase enzyme was formulated separately for the starter and grower periods. Experimental groups were divided into 2 main groups: low P [(0.39% P; …