Veterinary Medicine Commons^™

Expression And Tissue Distribution Of Extracellular Calcium-Sensing Receptor (Casr) Mrna In Chickens, Guangcun Deng, Jianmin Bi, Jian Qiao, Guiei He, Boning Wu, Miaojie Zhang, Nana Lv

Turkish Journal of Veterinary & Animal Sciences

Extracellular calcium-sensing receptor (CaSR) is a recently discovered G-protein-coupled receptor that may play a key role in regulating parathyroid hormone secretion and calcium homeostasis. In the present study, based on the reported sequence of the chicken CaSR gene (GenBank accession: XM_416491), specific primers were designed and synthesized, and CaSR gene mRNA expression and tissue distribution in chickens were analyzed using semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) analysis. It was observed that CaSR mRNA was expressed in the kidney and parathyroid to a greater degree than in the small intestine, oviduct, and brain (P < 0.01). Sequence analysis of the PCR products obtained from the 5 tissues showed that these cDNAs shared 100% homology with each other and the corresponding region of the reported CaSR cDNA. The results may provide a basis and additional insight for understanding the physiological function of CaSR in chickens.

The Effects Of Ichthyophthiriasis On Some Haematological Parameters In Common Carp, Malgorzata Witeska, Elzbieta Kondera, Katarzyna Lugowska

Turkish Journal of Veterinary & Animal Sciences

The present study examined the haematological effects of ichthyophthiriasis in common carp. Blood was collected from 4 groups of juvenile carp: 1. healthy; 2. with minor symptoms of ichthyophthiriasis; 3. with severe symptoms of ichthyophthiriasis (moribund); survivors of ichthyophthiriasis after a 3-week recovery period. Blood was subjected to a standard haematological procedure and the following parameters were evaluated: haematocrit (Ht), haemoglobin concentration (Hb), erythrocyte (RBC) and leukocyte (WBC) counts, mean cell volume (MCV), mean cell haemoglobin (MCH), and mean cell haemoglobin concentration (MCHC). The differential leukocyte count and thrombocyte count were calculated using stained blood smears. No change in Ht …

Molecular Characterization Of Ornithobacterium Rhinotracheale Isolated From Broiler Chicken Flocks In Iran, Mohammad Hassanzadeh, Vahid Karrimi, Neda Fallah, Iradj Ashrafi

Turkish Journal of Veterinary & Animal Sciences

The present study was designed to isolate Ornithobacterium rhinotracheale in broiler flocks in Iran and characterize it using biochemical, polymerase chain reaction assays, and phylogenetic analyses. A total of 150 tracheal swabs were collected from broiler chickens in slaughterhouses. Additionally, 150 lung and 150 tracheal tissue samples were taken from dead birds of broiler flocks that were developing symptoms of respiratory diseases. For PCR assay DNA was extracted from ORT isolates and also from tissue samples. ORT was isolated from 1 out of 150 swabs samples and 3 out of 300 total tissue samples. Four ORT isolates together with 7 …

Ochratoxin A-Induced Serum Biochemical Alterations In New Zealand White Rabbits (Oryctolagus Cuniculus), Masood Saleem Mir, Prabakar Dwivedi

Turkish Journal of Veterinary & Animal Sciences

Our objective was to study the effects of oral ochratoxin A (OT-A) intoxication in rabbits. New Zealand white rabbits of 8 weeks age, weighing 350-400 g, were utilized for the study. Rabbits were allotted randomly to 3 groups. Group 1 (6 animals) served as control. Groups 2 and 3 (12 animals each) were fed diets supplemented with 1000 and 2000 ppb OT-A, respectively, for 8 weeks. Blood samples were collected at weekly intervals from day 0 up to week 8 and sera were separated for biochemical analysis. Serum glucose and chloride levels showed marked progressive decrease whereas a marginal decrease …

Interaction Between Particle Sizes Of Alfalfa Hay And Corn Grain On Milk Yield, Milk Composition, Chewing Activity, And Ruminal Ph Of Dairy Cows, Mehmet Ali̇ Bal, E. Banu Büyükünal Bal

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to determine the interactions between alfalfa hay and corn grain particle sizes on nutrient intake, milk yield, milk composition, chewing activity, and ruminal pH of dairy cows. Alfalfa hay was chopped either at 4 cm (long; L) or 1 cm (short; S) theoretical length of cut. Particle size of corn grain was obtained by grinding either at 1 mm (coarse; C) or less than 1 mm (fine; F) mean particle size. Four lactating multiparous Holstein cows were assigned in a 4×4 Latin Square design in a 2×2 factorial arrangement of treatments with 21-day periods. …

A Case Study Of The Clinical Form Of Johne’S Disease In A Heifer, Agnieszka Wiszniewsk Laszczych, Joanna Szteyn, Aleksandra Ruszczynska

Turkish Journal of Veterinary & Animal Sciences

Johne's disease-paratuberculosis-affects domesticated and wild ruminants throughout the world. The first symptoms of the disease appear 6 months to 15 years after infection. The clinical stage of paratuberculosis usually occurs in older animals and is rare in those under 2 years of age. While performing analyses on a farm in the northeastern region of Poland a 2-year-old heifer that manifested symptoms of dyspepsia, proceeding with watery diarrhea, was observed. ELISA test (IDEXX) results demonstrated a positive serological reaction for paratuberculosis. Clinical examination, microbiological analysis of feces, autopsy, and histopathological examination confirmed the incidence of Johne's disease in the heifer.

The Effects Of L-Carnitine Administration On Energy Metabolism In Pregnant Halep (Damascus) Goats, Ci̇han Kaçar, Abuzer Kaffar Zonturlu, Mahmut Karapehli̇van, Umut Çağri Ari, Meti̇n Öğün, Mehmet Çi̇ti̇l

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of parenteral administration of L-carnitine on some biochemical parameters in Halep (Damascus) goats during the last month of pregnancy. L-carnitine was administrated to goats in group I (n = 13) by subcutaneous injections once a week during the last month of the pregnancy. Physiologic salt solution was administered to goats in group II (n = 12) by the same route during the same period. Differences of glucose concentration between groups were not significant (P > 0.05). Serum \beta-hydroxybutyric acid (BHB) concentrations in both groups increased until parturition. However, differences between groups …

The Effect Of Feeding Frequency On The Hormonal Profile, Carcass Characteristics, And Feedlot Performance In Iranian Holstein Calves, Armin Towhidi, Aboulfazl Zali, Abbas Khoshsokhan, Homayoon Khazali, Mehdi Zhandi, Kamran Rezayazdi, Reza Masoumi, Hamid Gholami

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to investigate the effect of feeding frequency on body weight, carcass characteristics, and hormonal profile in Iranian Holstein calves. The study included 12 Holstein bull calves that were assigned to 2 groups according to initial live body weight. The animals were fed a TMR ration based on live body weight. The calves were fed a standard diet for 31 weeks; control calves were fed twice a day and treatment calves were fed 7 times a day. Live body weights were recorded every 21 days. Blood samples were collected 1 h after the final …

Reproductive Performance Following Unilateral Ovariectomy For Treatment Of Ovarian Tumors In 7 Mares, Mehmet Can Gündüz, Güven Kaşikçi, Ragip Kiliçarslan, Meli̇h Uçmak, Oktay Düzgün, Çağatay Tek

Turkish Journal of Veterinary & Animal Sciences

Unilateral ovariectomies were performed on 7 mares under inhalation anesthesia for treatment of ovarian tumors. All mares were evaluated for a 2-year history of infertility; 6 mares showed persistent anestrus while 1 mare showed persistent estrus. The presumptive clinical diagnosis of the tumors was made by rectal palpation and ultrasonographic imaging of the ovaries. The affected ovaries were large, spherical, and hard, and the contralateral ovaries were small and inactive. The flank approach technique was used for all of the mares. All mares were operated on successfully. One of the mares had delayed healing and abscess formation in the incision …

Acute Pulmonary Emphysema Cum Pulmonary Edema Apparently Associated With Feeding Of Brassica Juncea In A Dairy Buffalo, Ghulam Muhammad, Muhammad Saqib, Abeera Naureen

Turkish Journal of Veterinary & Animal Sciences

This preliminary report describes the occurrence of acute pulmonary emphysema cum pulmonary edema ensuing in extensive subcutaneous emphysematous swellings in a dairy buffalo (Bubalus bubalis) apparently associated with a sudden shift from berseem (Trifolium alexendrinum) to Brassica juncea fodder. Tachypnea, expiratory dyspnea, open-mouth breathing, loud expiratory grunt with abdominal lift, and crackles in ventral aspects of the lungs with normal rectal temperature characterized the condition clinically. A substantial improvement was noted 3 h after treatment with parenteral administration of prednisolone plus dexamethasone, diclofenac sodium, and furosemide. Per os administration of chlortetracycline was undertaken after dyspnea had abated. Extensive subcutaneous swellings …

Evaluation Of Different Laparoscopic Sterilization Techniques In A Canine Birth Control Program, Avijit Dutta, Swapan Maiti, Ajith Parameswaran Pillai, Naveen Kumar

Turkish Journal of Veterinary & Animal Sciences

Three laparoscopic sterilization techniques under xylazine-ketamine anesthesia were evaluated at a 10 mmHg pressure gradient of CO_2 in 60 mongrel bitches, equally divided into 3 groups (A, B, and C). Laparoscopic bilateral oopherectomy was done in group A, laparoscopic ovariohysterectomy by endoclip and electrocautery in group B, and sterilization by electrocautery in group C. All of the animals recovered from anesthesia smoothly after the completion of laparoscopic surgery. Intraoperative observations revealed good visualization of the reproductive organs at 10 mmHg of pressure in the Trendelenburg position. Comparison among the various laparoscopic techniques revealed a more effective hemostasis with titanium endoclip …

Responses To Abrupt Changes In Feeding And Illumination In Laying Hens, Ashraf M. Khalil, Kanji Matsui, Ken-Ichi Takeda

Turkish Journal of Veterinary & Animal Sciences

This study aimed to investigate the responses to abrupt changes in feeding and illumination during the egg-laying period. Six hens were housed individually in cages under constant environmental conditions, with a photoperiod of 15 h (0400-1900) and ad libitum access to food for 10 days. Then the same hens were subjected to a feed withdrawal trial (between 1200 and 0830), followed by a 5-h reduction in the photoperiod (0400-1400). The heart rate (HR), body temperature (BT), and locomotor activity (LA) of the laying hens were measured continuously using radiotelemetry, with simultaneous recording of the time of oviposition. Behavioral responses to …

Intestinal Geotrichosis In A German Shepherd, Yajane Lee, Wei-Li Hsu, Chin-Fu Lin, Shao-Wei Lee, Feng-Pang Cheng

Turkish Journal of Veterinary & Animal Sciences

A 4-year-old sexually intact male German shepherd with a 3-month history of chronic watery diarrhea was referred to the Veterinary Medical Teaching Hospital. Dehydration and serum biochemistry revealed hypoalbuminemia, and fecal material contained a large quantity of round arthroconidia that were microscopically observed. A specimen was submitted for fungal culture and yielded a white to cream-colored subsurface colony. Microorganisms derived from the colony exhibited chains of smooth, septate hyaline hyphae that were producing 1-celled arthroconidia. Geotrichum candidum was thus identified. Colonoscopic features included erythema, edema, and loss of the usual fine vascular pattern, with granularity of the mucosa of the …

Michigan Bovine Tuberculosis: Annotated Bibliography

Michigan Bovine Tuberculosis Bibliography and Database

The bibliography includes 259 research publications from the years 1930-2010. It is international in scope, including articles relating to North and South America, Africa, Europe, Australia, and New Zealand. It includes journal articles, dissertations, government publications, reports, and proceedings. Each publication entry includes citation, abstract, and key words.

The bibliography (PDF) was generated from an MS Access database. A "zipped" version of that database is attached below as a related file (it is approximately 175 Mb)

Evaluation Of A Rapid Serological Test For The Determination Of Mycobacterium Bovis Infection In Badgers (Meles Meles) Found Dead, Mark A. Chambers, Konstantin P. Lyashchenko, Rena Greenwald, Javan Esfandiari, Eurig James, Leslie Barker, Jeff Jones, Gavin Watkins, Simon Rolfe

Michigan Bovine Tuberculosis Bibliography and Database

Between October 2005 and May 2006, a total of 727 badgers found dead in Wales were reported, and 550 were delivered to the Regional Laboratories of the Veterinary Laboratories Agency (VLA). Of the 459 carcasses suitable for examination, 55 were deemed to be infected with Mycobacterium bovis on the basis of culture, spoligotyping, and variable-number tandem repeat typing. Acid-fast bacteria were observed histologically in a further six badgers, but these bacteria were not confirmed as M. bovis by culture. A rapid serological test (BrockTB Stat-Pak) performed on thoracic blood showed a sensitivity of 35% and a specificity of 99%. Presence …

Estimating The Extent Of Spatial Association Of Mycobacterium Bovis Infection In Badgers In Ireland, G. E. Kelly, G. E. Mcgrath, S. J. More

Michigan Bovine Tuberculosis Bibliography and Database

Mycobacterium bovis infects the wildlife species badgers Meles meles who are linked with the spread of the associated disease tuberculosis (TB) in cattle. Control of livestock infections depends in part on the spatial and social structure of the wildlife host. Here we describe spatial association of M. bovis infection in a badger population using data from the first year of the Four Area Project in Ireland. Using second-order intensity functions, we show there is strong evidence of clustering of TB cases in each the four areas, i.e. a global tendency for infected cases to occur near other infected cases. Using …

Emergence Of Diseases From Wildlife Reservoirs, J. C. Rhyan, T. R. Spraker

Michigan Bovine Tuberculosis Bibliography and Database

Interest in the epidemiology of emerging diseases of humans and livestock as they relate to wildlife has increased greatly over the past several decades. Many factors, most anthropogenic, have facilitated the emergence of diseases from wildlife. Some livestock diseases have ‘‘spilled over’’ to wildlife and then ‘‘spilled back’’ to livestock. When a population is exposed to an infectious agent, depending on an interaction of factors involving the host, agent, and environment, the population may be resistant to infection or may become a dead-end host, a spillover host, or a maintenance host. Each exposure is unique; the same species of host …

Histomorphology Of The Oviduct Epithelium In The Angora Rabbit, Asuman Özen, Emel Ergün, Aytül Kürüm

Turkish Journal of Veterinary & Animal Sciences

The present study was undertaken to investigate cyclic changes in the structure of oviduct epithelial cells, and the content of oviductal mucus in the Angora rabbit using light and electron microscopy. Ten female Angora rabbits, 5 of which were in the estrual stage and the other 5 were in the luteal stage of the estrous cycle, were used in the study. Tissue samples taken from the fimbria, ampulla and isthmus of the oviduct were examined under light and electron microscope. Ciliated cells were demonstrated to be predominant in the fimbria and ampulla, whereas secretory cells were determined to be most …

Comparative Studies On The Sensitivity Of Polymerase Chain Reaction (Pcr) And Microscopic Examination For The Detection Of Trypanosoma Evansi In Horses, M.A. Muieed, Zafar Iqbal Chaudhry, A. R. Shakoori

Turkish Journal of Veterinary & Animal Sciences

The lack of reliable diagnostic methods is a setback in the effective control of diseases. Diagnosis of Trypanosoma evansi infection (surra) in livestock is of considerable economic importance. Detection of trypanosomes in blood has been the "gold standard"; however, finding the organisms or establishing patency of parasitaemia has not always been possible even in symptomatic infections. As a consequence of low dose infection, the pre-patent period in Trypanosoma evansi may be longer and, even when symptoms have developed, trypanosomes may still not be demonstrable in blood, thus delaying treatment and thereby increasing morbidity and mortality. Trypanosoma evansi, a protozoan parasite …

Effect Of A Direct-Fed Microbial (Eubios 1090) In The Presence Of Antibiotics (Carbadox Or Ctc-Denagard) On Post-Weaning Pig Growth Performance And Immune Response, Kimberley Santos, Charles Maxwell, Elizabeth Kegley, Charles Rosenkrans

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

A study was conducted to determine the effects of a probiotic (Eubios 1090), in the presence of two different antibiotics, on performance in nursery pigs. A total of 216 pigs were weaned at an average of 21 d, blocked by initial body weight (BW = 6.79 kg), and distributed into 32 pens of 6 to 7 pigs per pen in an offsite nursery facility. Pens were randomly assigned to 1 of 4 dietary treatments (8 pens per treatment) that were fed throughout post-weaning phase 1 (day (D) 0 to 10), phase 2 (D 10 to 20), and phase 3 (D …

Isolation And Cultivation Of Equine Corneal Keratocytes, Fibroblasts And Myofibroblasts, Dylan G. Buss, Elizabeth A. Giuliano, Ajay Sharma, Rajiv R. Mohan

Pharmacy Faculty Articles and Research

Objective—To establish an in vitro model for the investigation of equine corneal wound healing. To accomplish this goal, a protocol to isolate and culture equine corneal keratocytes, fibroblasts and myofibroblasts was developed.

Animal material—Equine corneal buttons were aseptically harvested from healthy research horses undergoing humane euthanasia for reasons unrelated to this study. Slit-lamp biomicroscopy was performed prior to euthanasia by a board-certified veterinary ophthalmologist to ensure that all samples were harvested from horses free of anterior segment disease.

Procedure—Equine corneal stroma was isolated using mechanical techniques and stromal subsections were then cultured. Customized media at different culture conditions was used …

Pathological And Clinical Findings And Tissue Distribution Of Salmonella Gallinarum Infection In Turkey Poults, Lati̇fe Beyaz, Ayhan Atasever, Fuat Aydin, K. Semi̇h Gümüşsoy, Seçi̇l Abay

Turkish Journal of Veterinary & Animal Sciences

This study aimed to experimentally induce Salmonella enterica subsp. enterica serovar Enteritidis (Salmonella Gallinarum) infection in turkey poults in order to detect S. Gallinarum using immunohistochemical, bacteriological, and histological procedures. The study included 90 white turkey poults that were divided into 3 groups (1-day-old, 3-week-old, and 2-month-old), each of which was orally inoculated with an inoculum of 0.3 mL of S. Gallinarum strain 9 broth culture that contained approximately 2 × 10^4, 1 × 10^6, and 1 × 10^9 cfu/mL, respectively. The duration of the experiment was 18 days. Clinical, pathological, and bacteriological findings were evaluated daily until 18 days …

Contemporary Grouping In Mixed-Size Dairy Herds Experiencing Four Seasons, Mohammad Ali̇ Nilforooshan

Turkish Journal of Veterinary & Animal Sciences

Countries with different herd structures and climates use different combinations of herd, year, and season to define the effect of their climatic conditions and management. Estimates of variance components and Akaike information criterion values resulting from 8 analytical models differentiated by different combinations of herd, year and season effects were compared for milk and fat yields. Data consisted of pedigree and records of 11,850 Holsteins freshening in 38 dairy herds of Isfahan, Iran, between January 1994 and October 2001. Heritability estimates were lowest for the models that used herd-year combination as a random effect. Moreover, the use of these models …

Immune Response To Type-2 Porcine Circovirus In Weaned Pigs, Hong Chen, Ke-Ying Zhang, Xue-Mei Ding, Zhi-Wen Xu, Dai-Wen Chen, Gang Tian

Turkish Journal of Veterinary & Animal Sciences

Sixteen crossbred barrows (Duroc × Landrace × Yorkshire) weaned at 28 days with an initial body weight of 5.12-8.64 kg and no antibodies to PCV2 were used in a 5-week trial to evaluate the immune response after intranasal inoculation with type-2 porcine circovirus. The percentage of peripheral blood ANAE+ T cells, PCV2 antibody titer, immunoglobulins, and serum IFN-\gamma, IL-2, and sIL-2R levels were determined. The results show that the percentage of peripheral blood ANAE+ T cells was notably higher in inoculated pigs 5 days post-inoculation (dpi) and significantly lower 21 dpi than in the control group. The anti-PCV2 titer in …

The Performance Of A Small-Scale, High-Density, Continuous System For Culturing The Rotifer Brachionus Plicatilis, Umur Önal, İhsan Çeli̇k, Sebahatti̇n Ergün

Turkish Journal of Veterinary & Animal Sciences

The performance of a high-density continuous system was evaluated as an alternative to conventional low-density batch-culture systems for culturing the rotifer Brachionus plicatilis. Rotifer densities ranging from 1700 to 3760 individuals/mL were achieved in 4 different trials that lasted 10-18 days. Problems associated with NH_3-N and pH were controlled by increasing the water exchange rate and periodic addition of ammonia remover. While NH_3-N and pH values were 0-8.8 mg/L and 6.9-9.3, respectively, the most important problem was the accumulation of TSS at concentrations of up to 85 mL/L within a very short time after start-up. Further studies are required to …

Reference Values For Some Haematological, Haematochemical, And Electrophoretic Parameters In The Girgentana Goat, Giuseppe Piccione, Stefania Casella, Lucio Lutri, Irene Vazzana, Vincenzo Ferrantelli, Giovanni Caola

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to determine haematological, haematochemical, and electrophoretic reference values for the Girgentana goat in order to form a basis for clinical interpretation. The study included 348 female Girgentana goats aged from 1 to 6 years. The animals were divided into 3 groups: goats aged 1-2 years (group A), goats aged 3-4 years (group B), and goats aged 5-6 years (group C). Blood samples were collected from each animal every morning at the same time (0800) by means of jugular venipuncture, and red blood cell (RBC), white blood cell (WBC), neutrophil (NEU), lymphocyte (LYM), monocyte (MONO), …

A Common Tortoise Tick, Hyalomma Aegyptium Linne 1758 (Acari: Ixodidae), Identified On Eastern Hedgehog (Erinaceus Concolor Martin 1838) In Central Anatolia, Serkal Gazyağci, Nursel Aşan, Yasi̇n Demi̇rbaş

Turkish Journal of Veterinary & Animal Sciences

Ticks are obligate blood sucker arthropods that infect animals and humans. A common tortoise tick, Hyalomma aegyptium, was collected from a young and an adult male hedgehog, Erinaceus concolor, from Central Anatolia in July 2008. More ticks were determined on the young one. This is the second record of tortoise tick that parasitizes a hedgehog.

Conception Rates For Standing Estrus And Fixed-Time Insemination In Dairy Heifers Synchronized With Gnrh And Pgf_{2\Alpha}, Samir Z. El-Zarkouny

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to evaluate the effectiveness of GnRH PGF-based protocols on reproductive outcome in Holstein heifers. Heifers were randomly assigned to the control group (n = 14), or treatment groups (n = 50) treated with GnRH (day -7) and PGF_{2\alpha} (day 0). Heifers that exhibited estrus were inseminated (Select Synch, n = 34) and heifers that did not exhibit estrus up to 48 h after PGF_{2\alpha} injection received a second GnRH injection and were fixed-time inseminated (Ovsynch, n = 16). Conception rates were 40%, 55.9%, and 31.3%, and overall pregnancy rates (P < 0.05) were 64.3%, 97%, and 87.5% in the control, Select Synch, and Ovsynch groups, respectively. Ovsynch heifers had 1.8 ± 0.5 and 3.2 ± 0.6 ng mL^{–1} of serum P4, versus 3.3 ± 0.5 and 3.8 ± 0.6 ng mL–1 in the Select Synch heifers at the time of the first GnRH and PGF_{2\alpha} injection, respectively. Conception rates were 55.6% and 58.3% when P4 concentrations were high, and were 50% and 25% when P4 concentrations were low at the time of the first GnRH and PGF_{2\alpha} injection, respectively. Dairy heifers synchronized with GnRH and PGF_{2\alpha} should be inseminated when estrus is observed, and heifers that do not exhibit estrus up to 48 h after PGF_{2\alpha} injection should be given a second GnRH injection and fixed-time inseminated 16-20 h later.

Effect Of Pre-Evisceration, Skin-On Carcass Decontamination Sanitation Strategies For Reducing Bacterial Contamination Of Cattle During Skinning, Mehmet Çalicioğlu, Dennis R. Buege, John B. Luchansky

Turkish Journal of Veterinary & Animal Sciences

The effectiveness of pre-evisceration, skin-on carcass sanitation on reducing bacterial contamination of beef carcasses was tested using 3 cattle per treatment and 3 cattle as controls at each of 3 abattoirs in southern Wisconsin. The sanitation procedure included stunning, bleeding, tying off the esophagus, sealing the anus, and then sanitizing the hide with: i) 20% trisodium phosphate, ii) 200 ppm iodophor, iii) 75% ethanol, or iv) hot water (ca. 80 °C). Two sets of combined sponge samples (3 × 100 cm^2) were taken from the hide before and after the sanitation step, as well as from the carcass after the …

All-Trans Retinoic Acid Regulates Intracellular Redox Status In Marc-145 Cells Infected With Porcine Reproductive And Respiratory Syndrome Virus, Yan Lin, De Wu

Turkish Journal of Veterinary & Animal Sciences

A relationship between oxidative stress, and apoptosis and/or death has been suggested for many years. The objective of this study was to determine the influence of all-trans retinoic acid (ATRA) on the cell survival rate and the antioxidant defense system. MARC-145 cells were treated with ATRA after infection with porcine reproductive and respiratory syndrome virus (PRRSV). The cell survival rate, total antioxidant capability (T-AOC), glutathione peroxidase (GSH-P_X) activity, superoxide dismutase (SOD) activity, lactate dehydrogenase (LDH) activity, and glutathione (GSH) content were determined in samples collected from cells incubated with ATRA for 24 or 48 h. The results show that PRRSV …