Veterinary Medicine Commons^™

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Cell-Type Specific Distribution Of Chloride Transporters In The Rat Suprachiasmatic Nucleus, Michael A. Belenky, Patricia J. Sollars, David B. Mount, Seth L. Alper, Yosef Yarom, Gary E. Pickard

School of Veterinary and Biomedical Sciences: Faculty Publications

The suprachiasmatic nucleus (SCN) is a circadian oscillator and biological clock. Cell-to-cell communication is important for synchronization among SCN neuronal oscillators and the great majority of SCN neurons use γ-aminobutyric acid (GABA) as a neurotransmitter, the principal inhibitory neurotransmitter in the adult central nervous system. Acting via the ionotropic GABAA receptor, a chloride ion channel, GABA typically evokes inhibitory responses in neurons via Cl− influx. Within the SCN GABA evokes both inhibitory and excitatory responses although the mechanism underlying GABA-evoked excitation in the SCN is unknown. GABA-evoked depolarization in immature neurons in several regions of the brain is a function …

Hair Whorls In The Dog (Canisfamiliaris). I. Distribution, Lisa M. Tomkins, Paul Mcgreevy

Anatomy Collection

Hair whorl characteristics were assessed in the domestic dog (Canis familiaris) in the regions of cephalic, cervical (dorsal, ventral, and lateral), thoracic and brachial axillary regions, the chest, shoulders, elbows, ventral abdominal region, and on the caudal thighs (ischiatic). They were classified as simple or tufted, and their position was recorded as the distance between their centers and bony landmarks within each region. The distribution of whorls was explored in a cohort of domestic dogs (N = 120) comprising a variety of breeds and cross‐breeds, sourced from shelters (N = 60) and the general public (N = 60). …

An Investigation Of Humeral Stress Fractures In Racing Thoroughbreds Using A 3d Finite Element Model In Conjunction With A Bone Remodeling Algorithm, Ryan James Moore

Master's Theses

The humerus of a racing horse Thoroughbred is highly susceptible to stress fractures at a characteristic location as a result of cyclic loading. The propensity of a Thoroughbred to exhibit humeral fracture has made equines useful models in the epidemiology of stress fractures. In this study, a racing Thoroughbred humerus was simulated during training using a 3D finite element model in conjunction with a bone remodeling algorithm. Nine muscle forces and two contact forces were applied to the 3-dimensional finite element model, which contains four separate load cases representing fore-stance, mid-stance, aft-stance, and standing. Four different training programs were incorporated …

The Duration Of The Effects Of Repeated Widespread Badger Culling On Cattle Tuberculosis Following The Cessation Of Culling, Helen E. Jenkins, Rosie Woodroffe, Christl A. Donnelly

Michigan Bovine Tuberculosis Bibliography and Database

Background: In the British Isles, control of cattle tuberculosis (TB) is hindered by persistent infection of wild badger (Meles meles) populations. A large-scale field trial—the Randomised Badger Culling Trial (RBCT)—previously showed that widespread badger culling produced modest reductions in cattle TB incidence during culling, which were offset by elevated TB risks for cattle on adjoining lands. Once culling was halted, beneficial effects inside culling areas increased, while detrimental effects on adjoining lands disappeared. However, a full assessment of the utility of badger culling requires information on the duration of culling effects.

Methodology/Principal Findings: We monitored cattle TB incidence in and …

The Minor Envelope Glycoproteins Gp2a And Gp4 Of Porcine Reproductive And Respiratory Syndrome Virus Interact With The Receptor Cd163, Phani B. Das, Phat X. Dinh, Israrul H. Ansari, Marcelo De Lima, Fernando A. Osorio, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

Porcine reproductive and respiratory syndrome virus (PRRSV) contains the major glycoprotein, GP5, as well as three other minor glycoproteins, namely, GP2a, GP3, and GP4, on the virion envelope, all of which are required for generation of infectious virions. To study their interactions with each other and with the cellular receptor for PRRSV, we have cloned each of the viral glycoproteins and CD163 receptor in expression vectors and examined their expression and interaction with each other in transfected cells by coimmunoprecipitation (co-IP) assay using monospecific antibodies. Our results show that a strong interaction exists between the GP4 and GP5 proteins, although …

Porcine Reproductive And Respiratory Syndrome Virus Nonstructural Protein 1Β Modulates Host Innate Immune Response By Antagonizing Irf3 Activation, Lalit Beura, Saumendra Sarkar, Byungjoon Kwon, Sakthivel Subramaniam, Clinton J. Jones, Asit K. Pattnaik, Fernando A. Osorio

School of Veterinary and Biomedical Sciences: Faculty Publications

Porcine reproductive and respiratory syndrome virus (PRRSV) infection of swine leads to a serious disease characterized by a delayed and defective adaptive immune response. It is hypothesized that a suboptimal innate immune response is responsible for the disease pathogenesis. In the study presented here we tested this hypothesis and identified several nonstructural proteins (NSPs) with innate immune evasion properties encoded by the PRRS viral genome. Four of the total ten PRRSV NSPs tested were found to have strong to moderate inhibitory effects on beta interferon (IFN-β) promoter activation. The strongest inhibitory effect was exhibited by NSP1 followed by, NSP2, NSP11, …

Dairy Animal Care: Quality Assurance

Nebraska Beef Quality Assurance Program

Dairy producers are encouraged to participate in the voluntary Dairy Animal Care and Quality Assurance (DACQA) Certification program. The purpose of this program is to enhance and demonstrate quality animal care practices, which assure food safety, quality and value as well as enhance consumer confidence in the milk and beef products that are harvested from cattle on America’s dairy farms.

Identification Of Parelaphostrongylus Odocoilei (Nematoda: Protostrongylidae) First-Stage Larvae In The Feces Of Gray Wolves (Canis Lupus) By Molecular Methods, Heather M. Bryan, Kathrin A. Sim, Chris T. Darimont, Paul C. Paquet, Brent Wagner, Violeta Muñoz-Fuentes, Judit E. Smits, Nell B. Chilton

Parasitology Collection

First-stage nematode larvae with a dorsal-spine (DSL) were detected in five of 1,565 fecal samples from gray wolves (Canis lupus) collected in British Columbia, Canada, between 2005 and 2008. Molecular techniques were used to identify the DSL because it was not possible to determine their species identity using morphologic characters. The DSL were identified as Parelaphostrongylus odocoilei based on the results of single-strand conformation polymorphism (SSCP) analyses and DNA sequencing of the ribosomal DNA first and second internal transcribed spacers. Finding DSL of P. odocoilei in the feces of gray wolves was unexpected because P. odocoilei adults are parasites of …

An Enzootic Vector-Borne Virus Is Amplified At Epizootic Levels By An Invasive Avian Host, Valerie A. O'Brien, Amy T. Moore, Ginger R. Young, Nicolas Komar, William K. Reisen, Charles R. Brown

Zoonotics and Wildlife Disease: Publications

Determining the effect of an invasive species on enzootic pathogen dynamics is critical for understanding both human epidemics and wildlife epizootics. Theoretical models suggest that when a naive species enters an established host–parasite system, the new host may either reduce (‘dilute’) or increase (‘spillback’) pathogen transmission to native hosts. There are few empirical data to evaluate these possibilities, especially for animal pathogens. Buggy Creek virus (BCRV) is an arthropod-borne alphavirus that is enzootically transmitted by the swallow bug (Oeciacus vicarius) to colonially nesting cliff swallows (Petrochelidon pyrrhonota). In western Nebraska, introduced house sparrows (Passer domesticus …

Molecular Genealogy Tools For White-Tailed Deer With Chronic Wasting Disease, Holly B. Ernest, Bruce R. Hoar, Jay A. Well, Katherine I. O'Rourke

Zoonotics and Wildlife Disease: Publications

Molecular genetic data provide powerful tools for genealogy reconstruction to reveal mechanisms underlying disease ecology. White-tailed deer (Odocoileus virginianus) congregate in matriarchal groups; kin-related close social spacing may be a factor in the spread of infectious diseases. Spread of chronic wasting disease (CWD), a prion disorder of deer and their cervid relatives, is presumed to be associated with direct contact between individuals and by exposure to shared food and water sources contaminated with prions shed by infected deer. Key aspects of disease ecology are yet unknown. DNA tools for pedigree reconstruction were developed to fill knowledge gaps in …

Experimental Oral Transmission Of Chronic Wasting Disease To Red Deer (Cervus Elaphus Elaphus): Early Detection And Late Stage Distribution Of Protease-Resistant Prion Protein, Aru Balachandran, Noel P. Harrington, James Algire, Andrei Soutyrine, Terry R. Spraker, Martin Jeffrey, Lorenzo González, Katherine I. O'Rourke

Zoonotics and Wildlife Disease: Publications

Chronic wasting disease (CWD), an important emerging prion disease of cervids, is readily transmitted by intracerebral or oral inoculation from deer-to-deer and elk-to-elk, suggesting the latter is a natural route of exposure. Studies of host range susceptibility to oral infection, particularly of those species found in habitats where CWD currently exists are imperative. This report describes the experimental transmission of CWD to red deer following oral inoculation with infectious CWD material of elk origin. At 18 to 20 months post-inoculation, mild to moderate neurological signs and weight loss were observed and animals were euthanized and tested using 3 conventional immunological …

Detection Of The Abnormal Isoform Of The Prion Protein Associated With Chronic Wasting Disease In The Optic Pathways Of The Brain And Retina Of Rocky Mountain Elk (Cervus Elaphus Nelsoni), T. R. Spraker, Katherine I. O'Rourke, T. Gidlewski, J. G. Powers, J. J. Greenlee, M. A. Wild

Zoonotics and Wildlife Disease: Publications

Eyes and nuclei of the visual pathways in the brain were examined in 30 Rocky Mountain elk (Cervus elaphus nelsoni) representing 3 genotypes of the prion protein gene PRNP (codon 132: MM, ML, or LL). Tissues were examined for the presence of the abnormal isoform of the prion protein associated with chronic wasting disease (PrP^CWD). Nuclei and axonal tracts from a single section of brain stem at the level of the dorsal motor nucleus of the vagus nerve were scored for intensity and distribution of PrP^CWD immunoreactivity and degree of spongiform degeneration. This obex scoring …

Prion Genotypes Of Scrapie-Infected Canadian Sheep 1998–2008, Noel P. Harrington, Katherine I. O'Rourke, Yuquin Feng, Jasmine Rendulich, Cathleen Difruscio, Aru Balachandran

Zoonotics and Wildlife Disease: Publications

This report describes the genetics of the prion protein gene (PRNP) at codons 136, 154, and 171 for sheep diagnosed with naturally acquired classical scrapie in Canada between 1998 and 2008. Genotyping analysis was performed on 249 sheep with confirmed classical scrapie infection representing 98 flocks from 6 provinces. A further case-control analysis of 3 of these flocks compared the genotypes between infected sheep (n = 72) and those of their healthy flockmates (n = 1990). The incidence of classical scrapie in the Canadian sheep population was highly associated with the ARQ haplotype (91.8%) and the …

Increased Risk Of Chronic Wasting Disease In Rocky Mountain Elk Associated With Decreased Magnesium And Increased Manganese In Brain Tissue, Stephen N. White, Katherine I. O'Rourke, Thomas Gidlewski, Kurt C. Vercauteren, Michelle R. Mousel, Gregory E. Phillips, Terry R. Spraker

Zoonotics and Wildlife Disease: Publications

Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) of Rocky Mountain elk in North America. Recent studies suggest that tissue and blood mineral levels may be valuable in assessing TSE infection in sheep and cattle. The objectives of this study were to examine baseline levels of copper, manganese, magnesium, zinc, selenium, and molybdenum in the brains of Rocky Mountain elk with differing prion genotypes and to assess the association of mineral levels with CWD infection. Elk with leucine at prion position 132 had significantly lower magnesium levels than elk with 2 copies of methionine. Chronic wasting disease-positive elk …

School Of Veterinary Medicine & Biomedical Sciences: 2010 Annual Report

School of Veterinary and Biomedical Sciences: Information and History

On September 4, 2009, The Department of Veterinary Medicine and Biomedical Sciences was renamed the School of Veterinary Medicine and Biomedical Sciences. The School is informally organized into six functional areas: • Undergraduate Teaching • Research and Graduate Studies • Extension • Veterinary Diagnostic Center (VDC) • Great Plains Veterinary Educational Center (GPVEC) • Professional Program in Veterinary Medicine (PPVM) While teaching and research activities bridge the entire faculty, subsets of the faculty are primarily involved with one area, i.e., the undergraduate program, the research and graduate program, the veterinary medical students clinical teaching at the GPVEC, the pre-clinical teaching …

Epizootiologic Survey Of Mycobacterium Bovis In Wildlife And Farm Environments In Northern Michigan, Gary W. Witmer, Amanda E. Fine, James Gionfriddo, Michael Pipas, Kirk Shively, Kim Piccolo, Patrick Burke

Michigan Bovine Tuberculosis Bibliography and Database

Bovine tuberculosis (bovine TB), caused by Mycobacterium bovis, has reemerged in northern Michigan, USA, with detections in white-tailed deer (Odocoileus virginianus) in 1994 and in cattle in 1998. Since then, significant efforts have been directed toward reducing deer densities in the area in the hopes of reducing the bovine TB prevalence rate in deer and eliminating spillover of the disease into cattle. Despite the success of the efforts to reduce deer densities, additional cattle herds have become infected. Other mammals can be infected with M. bovis, and some carnivores and omnivores had been found to be infected …

Comparative Evaluation Of Clinical Samples From Naturally Infected Dogs For Early Detection Of Canine Distemper Virus, Muhammad Zubair Shabbir, Masood Rabbani, Arfan Ahmad, Ali̇ Ahmed, Khushi Muhammad, Ikram Anwar

Turkish Journal of Veterinary & Animal Sciences

Early detection of canine distemper (CD) is critical for appropriate and timely treatment. The present study included 45 suspected cases with respiratory signs for a comparative evaluation of clinical samples in the early detection of canine distemper virus (CDV). Thirty-four dogs exhibited digestive system involvement, but none showed any neurological symptoms. Clinical progression of CD, based on age, sex, vaccination status, disease, and clinical findings, was observed. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect viral nucleoprotein RNA in plasma, conjunctival and nasal swabs. Only 10 dogs were positive for CDV infection. Among the 45 dogs, CDV RNA …

Collection And Preparation Of Milk Samples For Microbiological Culturing, Jeffrey M. Bewley, Robert J. Harmon, Stephan Locke

Agriculture and Natural Resources Publications

In developing individual farm mastitis control and treatment strategies, it is often necessary to characterize the types of bacteria that are present on your farm. To answer this question, a microbiological analysis, or milk culture, must be performed on milk samples collected from cows showing clinical or subclinical signs of mastitis. Results of the milk cultures will help identify which bacteria are causing the mastitis. In turn, this information can be used to alter mastitis control, prevention, and treatment options to fit your herd’s conditions.

During an investigation of a herd dealing with high somatic cell counts or a high …

Development Of A New Tacaribearenavirus Infection Model And Its Use To Explore Antiviral Activity Of A Novelaristeromycin Analog, Brian B. Gowen, M. H. Wong, D. Larson, W. Ye, K. H. Jung, E. J. Sefing, Ramona T. Skirpstunas, Donald F. Smee, John D. Morrey, S. W. Schneller

Animal, Dairy, and Veterinary Science Faculty Publications

Background

A growing number of arenaviruses can cause a devastating viral hemorrhagic fever (VHF) syndrome. They pose a public health threat as emerging viruses and because of their potential use as bioterror agents. All of the highly pathogenic New World arenaviruses (NWA) phylogenetically segregate into clade B and require maximum biosafety containment facilities for their study. Tacaribe virus (TCRV) is a nonpathogenic member of clade B that is closely related to the VHF arenaviruses at the amino acid level. Despite this relatedness, TCRV lacks the ability to antagonize the host interferon (IFN) response, which likely contributes to its inability to …

Alterations In Serum Tartrate-Resistant Acid Phosphatase And C-Terminal Telopeptide Of Type I Collagen In Experimental Canine Osteotomies Fixed Using 2 Different Techniques, Michail Paskalev, Svetozar Krastev

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to monitor serum tartrate-resistant acid phosphatase (TRAP) and C-terminal telopeptide fragments of type I collagen (CTX) over time in canine experimental osteotomies fixed using 2 osteosynthesis techniques, to determine the relevance of both markers in monitoring bone healing, and to investigate the influence of the 2 osteosynthesis techniques on their concentrations. Transperiosteal osteotomy of the diaphyses of the right tibia and fibula was performed in 12 dogs. The dogs were then randomly assigned to 1 of 2 groups: group 1 (6 dogs) underwent osteotomy fixed with intramedullary osteosynthesis (IMO) and group 2 (6 …

Anatomical Study Of The Brain Of The African Ostrich, Ke-Mei Peng, Yueping Feng, Gaoying Zhang, Huazhen Liu, Hui Song

Turkish Journal of Veterinary & Animal Sciences

The anatomical characteristics of the African ostrich brain were investigated in this study. The average weight, length, and width of the total brain are 26.34 g, 59.26 mm, and 42.30 mm, respectively. The cerebellum appears relatively well developed and obviously protrudes dorsally. The posterior superior part of the cerebellar vermis almost forms an angle of 130°. The ostrich brain has many more transverse fissures of the cerebellar vermis than do the brains of domestic fowls. Therefore, the surface area of the African ostrich's cerebellum is larger. The formation of the cerebrum is an obtuse triangle. Its surface is smooth, without …

A Comparitive Study On Traumatic Reticuloperitonitis And Traumatic Pericarditis In Egyptian Cattle, Mohamed M. Ghanem

Turkish Journal of Veterinary & Animal Sciences

This study was carried out on 15 Holstein cows divided into 3 groups of 5. Group 1 was the control, group 2 had traumatic reticuloperitonitis (TRP), and group 3 had traumatic pericarditis (TP). Comparison between the groups included clinical, hematological, biochemical, and ultrasonographic changes. TRP cows had arched backs, a sharp decrease in milk yield, and a reluctance to move, with abduction of the fore limbs. TP cows had edema of the brisket and submandibular region, with jugular vein distension and pulsation. TRP cows had a significant increase in PCV, leukocytes, and neutrophils, and a significant decrease in RBC, hemoglobin, …

Identification Of Babesia And Theileria Species In Small Ruminants In Central Anatolia (Turkey) Via Reverse Line Blotting, Abdullah İnci̇, Anil İça, Alparslan Yildirim, Önder Düzlü

Turkish Journal of Veterinary & Animal Sciences

This study was conducted between January 2006 and September 2008 to identify Babesia and Theileria species in sheep and goats in Central Anatolia via reverse line blotting (RLB). In total, 421 sheep and 152 goats were randomly selected from herds located in Kayseri, Yozgat, and Sivas provinces (all in central Turkey). The amplified hypervariable V4 region of the 18S rRNA gene of ovine piroplasm species, including Theileria lestoquardi, T. ovis, Babesia ovis, B. motasi, and B. crassa, was hybridized against species-specific probes. Theileria ovis and B. ovis were observed in the study animals. Among the 573 small ruminants included in …

The Effect Of A Single Administration Of Parenteral Oxytetracycline And Flunixin Meglumine Combination On The Reproductive Performance Of Dairy Cows With Subclinical Endometritis, Çağatay Tek, Ahmet Sabuncu, Serkan İki̇z, Funda Bağcigi̇l, Mehmet Can Gündüz, M. Ragip Kiliçarslan, Yakut Özgür

Turkish Journal of Veterinary & Animal Sciences

This study explored the effects of a single administration of flunixin meglumine (acting as a non-steroidal anti-inflammatory drug) and oxytetracycline combination on the reproductive performance of cows diagnosed with subclinical endometritis during the postpartum period. Cows aged 3 to 9 at 40 to 60 days postpartum were studied. Endometrial samples were collected from cows that had a uterine cornu (middle of the cornu) width of greater than 3.5 cm in the ultrasonographic examination, and 40 cows diagnosed with subclinical endometritis were included in the study. Cows in group 1 (n = 20) received a single dose of 20 mg of …

Evaluation Of The Efficacy Of Esterified Glucomannan, Sodium Bentonite, And Humic Acid To Ameliorate The Toxic Effects Of Aflatoxin In Broilers, Hasan Ghahri, Reza Habibian, Mehdi Abdollah Fam

Turkish Journal of Veterinary & Animal Sciences

A study was conducted to determine the efficacy of esterified glucomannan, sodium bentonite, and humic acid in counteracting the toxic effects of aflatoxin in a naturally contaminated diet fed to broilers. Seven-day-old chicks were randomly assigned to 1 of the 9 dietary treatments. The treatments were as follows: 1) control; 2) naturally contaminated diet with aflatoxin; 3, 4, 5, 6, and 7) naturally contaminated diet with aflatoxin supplemented with 0.2%, 0.4%, 0.6%, 0.8%, and 1.0% humic acid, respectively; 8 and 9) naturally contaminated diet supplemented with 0.5% sodium bentonite and 0.1% esterified glucomannan, respectively. Compared with the control, the naturally …

Preliminary Findings Of Behavioral Patterns In Captive Alpine Musk Deer (Moschus Sifanicus) And Prospects For Future Conservation, Xiuxiang Meng, Hong Yang, Qisen Yang, Zuojian Feng, Xu Peng, Genevieve C. Perkins

Turkish Journal of Veterinary & Animal Sciences

Captive farming of alpine musk deer (Moschus sifanicus) in China has been used for conservation and harvesting of musk since the mid 1950s. Despite this long history, management practices and captive breeding have been primarily based on trial and error due to lack of behavioral and ecological information about this vulnerable species. Understanding behavioral patterns plays a vital part in determining appropriate management systems; hence the aim of this study was to determine the effect of captivity on behavioral patterns of alpine musk deer by comparing wild-caught and the captive-born alpine musk deer. From August 2002 to January 2003, the …

Effect Of Urea And Oregano Oil Supplementation On Growth Performance And Carcass Characteristics Of Lamb Fed Diets Containing Different Amounts Of Energy And Protein, Önder Canbolat, Ali̇ Karabulut

Turkish Journal of Veterinary & Animal Sciences

The experiment was carried out using a 4 × 4 factorial design. There were 16 experimental groups. Each group consisted of 3 lambs. The experiment lasted 56 days. During the experiment, the oregano oil (0 and 5 g/kg DM) and urea (0, 6, 12, and 18 g/head per day) were given to lambs consuming low energy/low protein diets (2350 Kcal/kg DM/ 10.80% CP/DM, respectively) and normal energy/normal protein (2600 Kcal/kg DM, 14.97% CP/DM, respectively) diets. Daily body weight gain of lambs consuming normal energy/normal protein diets and supplemented with 6, 12 and 18 g/head per day urea were significantly (P …

First Reported Case Of Chironomid Midge Larva Infestations In Rainbow Trout (Oncorhynchus Mykiss Walbaum 1792) Eggs, Şevki̇ Kayiş, İlhan Altinok, Fi̇kri̇ Balta

Turkish Journal of Veterinary & Animal Sciences

Six days after transfer of eyed stage rainbow trout (Oncorhynchus mykiss) eggs into an incubator, egg mortality occurred during the winter of 2007. During the outbreak, approximately 30% of the eggs died. Rheocricotopus fuscipes (Kieffer, 1909) and Eukiefferiella claripennis (Lundbeck, 1898) (subfamily Orthocladiinae), Chironomid midge larvae were observed on the eggs by examining the eggs under the microscope. Histological sections of the infested eggs exhibited midge bite marks. This is the first report of Chironomid midges preying on fish eggs.

Blood Plasma Mineral Profile And Health Status In Postpartum Cows Fed An Anionic Diet Before Parturition, Cazim Crnkic, Salko Muratovic, Slavica Piplica, Aida Kavazovic, Stanimir Kutlaca

Turkish Journal of Veterinary & Animal Sciences

The effects of an anionic versus cationic (control) diet on plasma mineral levels and health status were investigated in 24 dry pregnant Holstein-Friesian cows during the last 3 weeks of pregnancy. The cows were in their third or fourth parity, had a moderate level of milk production in their previous lactation, and did not have a history of milk fever. The anionic diet significantly improved plasma calcium status, as compared to the control (2.18 vs. 1.99 mmol L^{-1}) and nonsignificantly reduced the incidence of subclinical hypocalcemia (1/12 vs. 5/12) without affecting other periparturient diseases. Other plasma mineral levels were unaffected, …