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2004

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Articles 61 - 90 of 289

Full-Text Articles in Veterinary Medicine

Identification Of A Novel Bovine Herpesvirus 1 Transcript Containing A Small Open Reading Frame That Is Expressed In Trigeminal Ganglia Of Latently Infected Cattle, Melissa Inman, Joe Zhou, Heather Webb, Clinton J. Jones May 2004

Identification Of A Novel Bovine Herpesvirus 1 Transcript Containing A Small Open Reading Frame That Is Expressed In Trigeminal Ganglia Of Latently Infected Cattle, Melissa Inman, Joe Zhou, Heather Webb, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine herpesvirus 1 (BHV-1), like other Alphaherpesvirinae subfamily members, establishes latency in sensory neurons. The latency-related (LR) RNA is abundantly expressed during latency, and expression of an LR protein is required for the latency reactivation cycle in cattle. Within LR promoter sequences, a 135-aminoacid open reading frame (ORF) was identified, ORF-E, that is antisense to the LR RNA. ORF-E is also downstream of the gene encoding the major viral transcriptional activator, bICP0. Strand-specific reverse transcription-PCR demonstrated that a transcript containing ORF-E was consistently expressed in trigeminal ganglia (TG) of latently infected calves, productively infected cultured cells, and acutely infected calves. …

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Animal Health Matters, David H. Zeman Apr 2004

Animal Health Matters, David H. Zeman

Animal Health MATTERS Newsletter

Head/Director's Message [Page] 1-Equipment grant to support bioterrorism preparedness and TSE testing at the ADRDL [Page] 1- Call for Cases for the Summer Meeting Diagnostic News [Page] 2- BVD ear notch test – submission guidelines [Page] 2- Tritrichomonas pouch culture procedures at the ADRDL [Page] 2- Equine infectious anemia testing Research News [Page] 3- VSD and the Governor’s 2010 Research Initiative Extension News [Page] 3-Toxoplasma gondii and schizophrenia? [Page] 4- A survey of agents associated with neonatal diarrhea in Iowa swine including C. difficile and PRRSV [Page] 4- Avian influenza: should we be concerned about poultry in Asia?

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“French Heartworm” Infection Of Dogs & Foxes In Newfoundland Apr 2004

“French Heartworm” Infection Of Dogs & Foxes In Newfoundland

Other Publications in Zoonotics and Wildlife Disease

French Heartworm is the common name for a parasitic disease affecting foxes, dogs, and other members of the dog family. Caused by the nematode (worm) Angiostrongylus vasorum, this disease was first thought to exist in France in the mid 1800's. It is now considered to exist generally in Europe but has not been reported in North America other than on the Island of Newfoundland, with the exception of infected animals imported from Europe but diagnosed in North America. The first case recorded in Newfoundland was in Aquaforte (Avalon Peninsula) in 1973.
When and how it might have come over …

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Seroprevalence Of Bartonella Infection In American Free-Ranging And Captive Pumas (Felis Concolor) And Bobcats (Lynx Rufus), Bruno B. Chomel, Yoko Kikuchi, Janice S. Martenson, Melodie E. Roelke-Parker, Chao-Chin Chang, Rickie W. Kasten, Janet E. Foley, John Laudre, Kerry Murphy, Pamela K. Swift, Vicki L. Kramer, Stephen J. O'Brien Apr 2004

Seroprevalence Of Bartonella Infection In American Free-Ranging And Captive Pumas (Felis Concolor) And Bobcats (Lynx Rufus), Bruno B. Chomel, Yoko Kikuchi, Janice S. Martenson, Melodie E. Roelke-Parker, Chao-Chin Chang, Rickie W. Kasten, Janet E. Foley, John Laudre, Kerry Murphy, Pamela K. Swift, Vicki L. Kramer, Stephen J. O'Brien

Other Publications in Zoonotics and Wildlife Disease

Bartonella henselae is the main agent of cat scratch disease in humans and domestic cats are the main reservoir of this bacterium. We conducted a serosurvey to investigate the role of American wild felids as a potential reservoir of Bartonella species. A total of 479 samples (439 serum samples and 40 Nobuto strips) collected between 1984 and 1999 from pumas (Felis concolor) and 91 samples (58 serum samples and 33 Nobuto strips) collected from bobcats (Lynx rufus) in North America, Central America and South America were screened for B. henselae antibodies. The overall prevalence of B. …

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Genomically Intact Endogenous Feline Leukemia Viruses Of Recent Origin, Alfred L. Roca, Jill Pecon-Slattery, Stephen J. O'Brien Apr 2004

Genomically Intact Endogenous Feline Leukemia Viruses Of Recent Origin, Alfred L. Roca, Jill Pecon-Slattery, Stephen J. O'Brien

Biology Faculty Articles

We isolated and sequenced two complete endogenous feline leukemia viruses (enFeLVs), designated enFeLV-AGTT and enFeLV-GGAG. In enFeLV-AGTT, the open reading frames are reminiscent of a functioning FeLV genome, and the 5′ and 3′ long terminal repeat sequences are identical. Neither endogenous provirus is genetically fixed in cats but polymorphic, with 8.9 and 15.2% prevalence for enFeLV-AGTT and enFeLV-GGAG, respectively, among a survey of domestic cats. Neither provirus was found in the genomes of related species of the Felis genus, previously shown to harbor enFeLVs. The absence of mutational divergence, polymorphic incidence in cats, and absence in related species suggest that …

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Chronic Wasting Disease—Prion Disease In The Wild, Steve Bunk Apr 2004

Chronic Wasting Disease—Prion Disease In The Wild, Steve Bunk

Other Publications in Zoonotics and Wildlife Disease

In 1967, mule deer in a research facility near Fort Collins, Colorado, in the United States apparently began to react badly to their captivity. At least, that was the guess of researchers working on the natural history and nutrition of the deer, which became listless and showed signs of depressed mood, hanging their heads and lowering their ears. They lost appetite and weight. Then they died—of emaciation, pneumonia, and other complications—or were euthanized. The scientists dubbed it chronic wasting disease (CWD), and for years they thought it might be caused by stress, nutritional deficiencies, or poisoning. A decade later, CWD …

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A Mutation In The Latency-Related Gene Of Bovine Herpesvirus 1 Inhibits Protein Expression From Open Reading Frame 2 And An Adjacent Reading Frame During Productive Infection, Yanjun Jiang, Melissa Inman, Yange Zhang, Nuria Aleman Posadas, Clinton J. Jones Mar 2004

A Mutation In The Latency-Related Gene Of Bovine Herpesvirus 1 Inhibits Protein Expression From Open Reading Frame 2 And An Adjacent Reading Frame During Productive Infection, Yanjun Jiang, Melissa Inman, Yange Zhang, Nuria Aleman Posadas, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

The latency-related (LR) gene of bovine herpesvirus 1 (BHV-1) is abundantly expressed during latency. A mutant BHV-1 strain that contains three stop codons at the 5′ terminus of the LR gene (LR mutant) does not reactivate from latency. This study demonstrates that the LR mutant does not express open reading frame 2 or an adjacent reading frame that lacks an initiating ATG (reading frame C). Since the LR mutant and wild-type BHV-1 express similar levels of LR RNA, we conclude that LR protein expression plays an important role in regulating the latency reactivation cycle in cattle.

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Tuberculosis, John B. Kaneene, Charles O. Thoen Mar 2004

Tuberculosis, John B. Kaneene, Charles O. Thoen

Michigan Bovine Tuberculosis Bibliography and Database

Tuberculosis is a term that encompasses various diseases caused by bacteria of the Mycobacterium tuberculosis complex, including M tuberculosis, M bovis, M africanum, and other mycobacterial species. Whereas M tuberculosis infection is largely spread from human to human, M bovis infection has been identified as a zoonotic disease with most cases of human infection attributable to animal sources. The mycobacteria other than tuberculosis complex (MOTT), which includes M avium subsp avium and M avium subsp intracellulare isolated from animals, has been isolated from immune-compromised humans (ie, those with human immunodeficiency virus [HIV] infection), but seldom from immunocompetent humans. Recently, …

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Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn Mar 2004

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

No abstract provided.

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Ultrasound Diagnosis, Phiwipha Kamonrat Mar 2004

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

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What Is Your Diagnosis?, Pranee Tuntivanich Mar 2004

What Is Your Diagnosis?, Pranee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

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Evaluation Of The Aflatoxin B1 Adsorption Capacity Of Bentonite Using An In Vitro Method Mimicking Monogastric Gastro-Intestinal Tract Conditions, Piyarat Chansiripornchai, Johanna Fink-Gremmels Mar 2004

Evaluation Of The Aflatoxin B1 Adsorption Capacity Of Bentonite Using An In Vitro Method Mimicking Monogastric Gastro-Intestinal Tract Conditions, Piyarat Chansiripornchai, Johanna Fink-Gremmels

The Thai Journal of Veterinary Medicine

Aflatoxins are considered as unavoidable contaminants of food and animal feeds, since their production cannot be prevented successfully using current agricultural practices. The use of aflatoxin adsorbents as feed supplements is one of the most promising approaches to reduce aflatoxicoses in animals. Since there are numerous adsorbents marketed, an effective and practical method for the rapid evaluation of adsorption capacity of individual products is needed. In this study, we used an in vitro model designed to mimic the temperature, pH and the time of passage through the stomach and the intestinal tract of a monogastric animal, for the evaluation of …

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Peracute Infection Of Actinobacillus Pleuropneumoniae Serotype 1 In Guinea Pigs, Anongnart Somchit, Pornchalit Assavacheep, Wantanee Neramitmansook, Achariya Sailasuta Mar 2004

Peracute Infection Of Actinobacillus Pleuropneumoniae Serotype 1 In Guinea Pigs, Anongnart Somchit, Pornchalit Assavacheep, Wantanee Neramitmansook, Achariya Sailasuta

The Thai Journal of Veterinary Medicine

This experiment was performed to study gross and microscopic lung lesions after endotracheal inoculation with Actinobacillus pleuropneumoniae (A. pleuropneumoniae) serotype 1 in twenty-four guinea pigs. Four-six hours post inoculation, clinical signs of respiratory distress and epistasis were observed. Most of the infected animals suddenly died during this time. The differences between the pulmonary lesions were evaluated. In this experiment, the lesions of peracute infection by A. pleuropneumoniae were induced within 4-6 hours and the lesions of subacute infection, characterized by fibrinopurulent pleuritis were observed in surviving animals 18-20 hours post inoculation. The results suggested that guinea pigs could be used …

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Semiquantitative Analysis Of Il-10 Production In Bronchoalveolar Lavage Leukocytes Of Prrsv Infected Pigs, Permsak Thanawang, Chanida Yamkleebbua, Kritkong Shiyun, Roongroje Thanawongnuwech, Sawang Kesdangsakonwut, Supradit Wangnaitham, Sanipa Suradhat Mar 2004

Semiquantitative Analysis Of Il-10 Production In Bronchoalveolar Lavage Leukocytes Of Prrsv Infected Pigs, Permsak Thanawang, Chanida Yamkleebbua, Kritkong Shiyun, Roongroje Thanawongnuwech, Sawang Kesdangsakonwut, Supradit Wangnaitham, Sanipa Suradhat

The Thai Journal of Veterinary Medicine

Semi-quantitative analysis of the interleukin 10 (IL-10) in bronchoalveolar lavage cells (BALC), from pigs challenged with porcine reproductive and respiratory syndrome virus (PRRSV), was undertaken in this study. Twenty-one, three weeks old, crossbred pigs from a PRRSV-free commercial farm were separated into three groups, a control, non-infected group (3 pigs/group), and 2 groups (9 pigs/group), the first inoculated with a Thai-isolated European (O2SB3) strain and the second a Thai-isolated, North American (O1NP1) PRRSV strain. BALC were isolated from three infected pigs and one control on days 5, 9 and 15 post infection (DPI). The levels of IL-10 production in BALC …

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Survival Rates, Growth Rates, Weaning Weights And The Impact Of A Colostrum Supplement In Low Birthweight Piglets, Kangsadarn Pinsumrit, Morakot Suparattanasit, Arisa Oponsawat, Padet Tummaruk, Annop Kunavongkrit Mar 2004

Survival Rates, Growth Rates, Weaning Weights And The Impact Of A Colostrum Supplement In Low Birthweight Piglets, Kangsadarn Pinsumrit, Morakot Suparattanasit, Arisa Oponsawat, Padet Tummaruk, Annop Kunavongkrit

The Thai Journal of Veterinary Medicine

The objective of the present study was to investigate survival rates, growth rates, weaning weights and the effect of colostrum in low birth weight piglets. The study was made on 288 piglets with a birth weight below 1 kg. After the individual birth weights were measured, the piglets were divided into 2 treatment groups, a control group (the piglets received colostrums after birth from their dams according to the usual management practice) and a colostrum supplemented group (piglets received an extra 20 ml of colostrum within 12 hrs of birth). The study showed that the survival rate of the low …

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Karyotype Of Crossbred Progeny Born To The Interspecies Hybridization Of Wild And Zebu Cattle With Reference To Their Fertility And Growth Performance, Nussara Vadhanakul, Wanchai Tunwattana, Panpilai Sekkasiddhi, Montakarn Tansatit, Vivat Chavananikul Mar 2004

Karyotype Of Crossbred Progeny Born To The Interspecies Hybridization Of Wild And Zebu Cattle With Reference To Their Fertility And Growth Performance, Nussara Vadhanakul, Wanchai Tunwattana, Panpilai Sekkasiddhi, Montakarn Tansatit, Vivat Chavananikul

The Thai Journal of Veterinary Medicine

Karyotypes of the crossbred progeny of wild and zebu cattle, born from the insemination of zebu dams with deep frozen semen from gaur (Bos gaurus) and banteng (Bos javanicus) sires were studied. The crossbred progeny (F1) born from the gaur sire and zebu dams revealed a diploid chromosome number of 2n=58. Fifty-four acrocentric and 2 submetacentric autosomes were found, sex chromosomes being submetacentric and metacentric in the male and submetacentric in the female. Two types of chromosomes with a complement of 2n=59 and 2n=58, were found in the crossbred progeny (F1) of the banteng sire and zebu dams. Robertsonian translocation …

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The Determination Of Plasma Norepinephrine And Epinephrine In Dairy Cows And Swamp Buffaloes (Bubalus Bubalis) Using High Performance Liquid Chromatography With Electrochemical Detection, Sarinee Kalandakanond, Siripen Komolvanich, Sutthasinee Poonyachoti, Chollada Buranakarl Mar 2004

The Determination Of Plasma Norepinephrine And Epinephrine In Dairy Cows And Swamp Buffaloes (Bubalus Bubalis) Using High Performance Liquid Chromatography With Electrochemical Detection, Sarinee Kalandakanond, Siripen Komolvanich, Sutthasinee Poonyachoti, Chollada Buranakarl

The Thai Journal of Veterinary Medicine

In the present study, we report on the level of norepinephrine and epinephrine in healthy cattle, using high-performance liquid chromatography with an electrochemical detector (HPLC-EC). Sixteen crossbred Holstein dairy cows (> 87.5% Holstein) and fifteen swamp buffaloes (Bubalus bubalis), which included both sexes, were included. In the dairy cows, the plasma norepinephrine and epinephrine concentrations (mean ± SE) were 3.89 ± 0.51 nM and 0.82 + 0.12 nM, respectively. The concentrations of plasma norepinephrine and epinephrine in buffaloes (mean ± SE) were 3.75 ± 0.51 and 1.82 ± 0.45 nM, respectively.

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Acetylcholinesterase Activity And Total Haemocyte Count In Black Tiger Shrimps (Penaeus Monodon) Exposed To Trifluralin At Therapeutic Levels For Ectoparasitic Treatment, Phanupong Chaowanit, Apirak Pleanplak, Rujira Sangarun, Lila Ruangpan, Janenuj Wongtavatchai, Piyarat Chansiripornchai Mar 2004

Acetylcholinesterase Activity And Total Haemocyte Count In Black Tiger Shrimps (Penaeus Monodon) Exposed To Trifluralin At Therapeutic Levels For Ectoparasitic Treatment, Phanupong Chaowanit, Apirak Pleanplak, Rujira Sangarun, Lila Ruangpan, Janenuj Wongtavatchai, Piyarat Chansiripornchai

The Thai Journal of Veterinary Medicine

Trifluralin, a herbicide, was observed for it's side effects when used, extralabel, as an ectoparasiticide in shrimp farming. Black tiger shrimp were exposed to trifluralin at a therapeutic level, commonly employed in shrimp farming, 0.1 or 0.2 ppm, for up to 96 hrs. Shrimp were examined 96 hrs after the primary dosage of trifluralin. The total haemocyte count in the exposed shrimp was not different from the control, unexposed shrimp. A significant reduction of muscle acetylcholinesterase was found in all exposed shrimp (p<0.05). Some of the exposed shrimp also demonstrated clinical signs of cholinergic overload characterized as abnormal movement. This study suggests sublethal effects when trifluralin is used for the treatment of shrimp ectoparasitic disease.

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Genetic Polymorphism Of Beta And Kappa Casein In Crossbred Dairy Cattle, Chortip Aroondechachai, Chancharat Reodecha, Duangsmorn Suwattana, Monchai Duangjinda Mar 2004

Genetic Polymorphism Of Beta And Kappa Casein In Crossbred Dairy Cattle, Chortip Aroondechachai, Chancharat Reodecha, Duangsmorn Suwattana, Monchai Duangjinda

The Thai Journal of Veterinary Medicine

The objective of this study was to determine the frequencies of the genetic variants of the beta and kappa casein gene in 87 crossbred dairy cattle. Beta and kappa casein genotypes were investigated, the first by an allele-specific polymerase chain reaction (ASPCR) and the second by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Three beta casein alleles of A1, A2 and B were found, with frequencies of 0.30, 0.61 and 0.09, respectively. In total, five genotypes of A1 A1, A1 A2, A2 A2, A2B and A1B were found, with frequencies of 0.06, 0.41, 0.36, 0.11 and 0.06. Three kappa …

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Escherichia Coli 0 1 57:H7: An Update On Intestinal Colonization And Virulence Mechanisms, Rodney A. Moxley Mar 2004

Escherichia Coli 0 1 57:H7: An Update On Intestinal Colonization And Virulence Mechanisms, Rodney A. Moxley

School of Veterinary and Biomedical Sciences: Faculty Publications

Cattle are a major reservoir of Escherichia coli 0157:H7, an important zoonotic pathogen that causes hemorrhagic colitis and hemolytic uremic syndrome (HUS). Colonization of cattle occurs predominantly in the large intestine, and may especially target follicle-associated epithelium (FAE) in the terminal rectum. Bacterial colonization involves induction of attaching- effacing (A/E) lesions, mediated by type III secreted proteins and an outer membrane protein called intimin. ToxB, encoded on plasmid p0157, contributes to adherence of E. coli 0157:H7 through promotion of the production and/or secretion of type III secreted proteins. Introduction of type III secreted proteins and intestinal colonization appear to involve …

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Tape Worm Joyeuxiella Sp. Infection In A Cat: A Case Report, Sudchit Chungpivat, Anudep Rungsipipat, Komkrich Tienkum Mar 2004

Tape Worm Joyeuxiella Sp. Infection In A Cat: A Case Report, Sudchit Chungpivat, Anudep Rungsipipat, Komkrich Tienkum

The Thai Journal of Veterinary Medicine

A four years old, mixed breed cat was reported to have a Joyeuxiella sp. tapeworm infection. Upon necropsy, The most striking lesion was a diffuse chronic catarrhal enteritis along with a heavy tapeworm infection in the small intestine. The tapeworms were identified as the genus Joyeuxeilla, Family Dilepididae, based on the morphology which were comprised of 2 sets of reproductive organs in a mature segment and only one egg in each egg capsule, in the gravid segments. This is the first report of Joyeuxiella sp. tapeworm infection in Thailand.

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Effects Of Beta And Kappa Casein On Breeding Values Predictions In Dairy Cattle, Chortip Aroondechachai, Chancharat Reodecha, Monchai Duangjinda, Duangsmorn Suwattana Mar 2004

Effects Of Beta And Kappa Casein On Breeding Values Predictions In Dairy Cattle, Chortip Aroondechachai, Chancharat Reodecha, Monchai Duangjinda, Duangsmorn Suwattana

The Thai Journal of Veterinary Medicine

The objective of this study was to estimate the genetic parameters and breeding values for milk yield, protein yield and protein percentage in 87 crossbred dairy cattle with pedigree and production records and beta and kappa casein genotypes. The data was analyzed using three mixed model equations. The first model included fixed effects and random effects, identifying additive and permanent environments. The second model was similar to the first model using beta (a) or kappa (b) casein genotype (single gene) effect. The third model was the same as the first model but used composite beta and kappa casein genotype (multigene) …

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The Structure Of Red Blood Cells, White Blood Cells And Thrombocytes In Freshwater Crocodiles (Crocodylus Siamensis), Kamol Sakulwira, Wutichai Klomklew, Siripen Vethchagarun, Wimon Pothiwong Mar 2004

The Structure Of Red Blood Cells, White Blood Cells And Thrombocytes In Freshwater Crocodiles (Crocodylus Siamensis), Kamol Sakulwira, Wutichai Klomklew, Siripen Vethchagarun, Wimon Pothiwong

The Thai Journal of Veterinary Medicine

Blood cells and thrombocytes from five crocodiles, three males and two females, were studied by both light and transmission electron microscopes (TEM). The blood cells could be categorized into red blood cells, white blood cells and thrombocytes. The nucleated red blood cells were oval in shape and the TEM study revealed vacuoles in the cytoplasm. The white blood cells fell into two groups; one with granules and the other without granules. The granulocytes were heterophils, eosinophils and basophils. The heterophils had an eccentrically oval nucleus and a large number of acidophilic rod-shaped granules in the cytoplasm. Eosinophils also posessed an …

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Antigen Distribution Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) In Thai Crossbred Pigs Using Immunohistochemistry, Porntip Laohasittikul, Nuttapon Boonarpha, Yongyut Pongprapachuen, Sawang Kesdangsakonwut, Supradit Wangnaitham, Roongroje Thanawongnuwech Mar 2004

Antigen Distribution Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) In Thai Crossbred Pigs Using Immunohistochemistry, Porntip Laohasittikul, Nuttapon Boonarpha, Yongyut Pongprapachuen, Sawang Kesdangsakonwut, Supradit Wangnaitham, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Twenty, 3-week-old, crossbred pigs from a herd free from porcine reproductive and respiratory syndrome virus (PRRSV) were inoculated intranasally and intramusculary (1 ml each 102.5 TCID50/ml) with Thai PRRSV isolates of the US genotype (01NP1) (n=9) or the EU genotype (02SB3) (n=8). Three pigs were used as negative controls. Three infected and one control group pig were euthanatized 5, 9 or 15 days postinoculation (DPI) to detect and to compare the location and the amount of PRRSV-antigen present. Immunohistochemical evaluation of formalin-fixed tissues revealed PRRSV-antigen in the pulmonary macrophages of the lungs (6/9, 8/8 and 0/3 of the 01NP1-infected, 02SB3-infected …

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Chronic Wasting Disease Management In Nebraska Jan 2004

Chronic Wasting Disease Management In Nebraska

Other Publications in Zoonotics and Wildlife Disease

The presence of Chronic Wasting Disease (CWD) in free-roaming and domesticated cervine animal populations of deer and elk in the state of Nebraska presents a threat to the natural resources of the state. The presence of this disease could drastically restrict the successful achievement of the goals and objectives of the Nebraska Game and Parks Commission (NGPC) as set forth in their long range plan “Focusing on the Future…A Plan for Nebraska’s Fish, Wildlife and Parkland Resources”. Objective 3 in the Big Game section of this plan is to “Monitor the status and health of deer, so reasonable assumptions may …

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Decreased Shedding Of Escherichia Coli O157:H7 By Cattle Following Vaccination With Type Iii Secreted Proteins, Andrew A. Potter, Sandra Klashinsky, Yuling Li, Elizabeth Frey, Hugh Townsend, Dragan Rogan, Galen E. Erickson, Susanne Hinkley, Terry J. Klopfenstein, Rodney A. Moxley, David R. Smith, B. Brett Finlay Jan 2004

Decreased Shedding Of Escherichia Coli O157:H7 By Cattle Following Vaccination With Type Iii Secreted Proteins, Andrew A. Potter, Sandra Klashinsky, Yuling Li, Elizabeth Frey, Hugh Townsend, Dragan Rogan, Galen E. Erickson, Susanne Hinkley, Terry J. Klopfenstein, Rodney A. Moxley, David R. Smith, B. Brett Finlay

School of Veterinary and Biomedical Sciences: Faculty Publications

Cattle are an important reservoir of Escherichia coli O157:H7 leading to contamination of food and water, and subsequent human disease. This pathogen colonizes its hosts by producing several proteins such as Tir and EspA that are secreted by a type III secretion system. These proteins play a role in colonization of the intestine, suggesting that they might be useful targets for the development of a vaccine to reduce levels of this organism in cattle. Vaccination of cattle with proteins secreted by E. coli O157:H7 significantly reduced the numbers of bacteria shed in feces, the numbers of animals that shed, and …

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Department Of Veterinary And Biomedical Sciences 2004 Annual Report Jan 2004

Department Of Veterinary And Biomedical Sciences 2004 Annual Report

Veterinary and Biomedical Sciences -- Department Information and History

Veterinary and Biomedical Sciences Personnel
VBMS Teaching Program
VBMS Research Program
International Activities, 2004
Veterinary Extension Program. 2004
VBMS Grants and Contracts Program. 2004
Refereed Publications by VBMS Faculty in 2004
Department of Veterinary and Biomedical Sciences. Selected Committees, Editorial and Other Appointments. 2004
Articles Regarding the Department in 2004
Departmental Budget Summaries. 2004
Nebraska Agricultural Statistics. 2003/2004

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A Processed Pseudogene Contributes To Apparent Mule Deer Prion Gene Heterogeneity, Kelly A. Brayton, Katherine I. O'Rourke, Amy K. Lyda, Michael W. Miller, Donald P. Knowles Jr. Jan 2004

A Processed Pseudogene Contributes To Apparent Mule Deer Prion Gene Heterogeneity, Kelly A. Brayton, Katherine I. O'Rourke, Amy K. Lyda, Michael W. Miller, Donald P. Knowles Jr.

Other Publications in Zoonotics and Wildlife Disease

Pathogenesis and transmission of the prion disorders (transmissible spongiform encephalopathies, TSEs) are mediated by a modified isoform of the prion protein (PrP). Prion protein gene (PRNP) alleles associated with relative susceptibility to TSE have been identified in sheep, humans and possibly elk. Comparable data have not been derived for mule deer, a species susceptible to the TSE chronic wasting disease (CWD). Initial analysis of the open reading frame (ORF) in exon 3 of the mule deer PRNP gene revealed polymorphisms in all 145 samples analyzed, with 10 potential polymorphic sites. Because 144/145 (99.3%) of the samples were heterozygous …

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Histamine As An Aid To Biopsy Of Third Eyelid Lymphoid Tissue In Sheep, S. Bender, J. Alverson, L. M. Herrmann, Katherine I. O'Rourke Jan 2004

Histamine As An Aid To Biopsy Of Third Eyelid Lymphoid Tissue In Sheep, S. Bender, J. Alverson, L. M. Herrmann, Katherine I. O'Rourke

Other Publications in Zoonotics and Wildlife Disease

TRANSMISSIBLE spongiform encephalopathies, of which ovine scrapie is a prototype, are neurodegenerative disorders characterised by the accumulation of an abnormal isoform (PrP-scrapie or PrPSc) of a normal cellular protein (PrPC) (Prusiner 1982). Characteristics of the disease include long incubation periods, and a progressive and chronic clinical course resulting in death (Fraser 1976). PrPsc is detectable during the preclinical stages of scrapie in peripheral lymph nodes (Ikegami and others 1991, Muramatsu and others 1993), in lymphoid follicles of the tonsils (Schreuder and others 1996) and in lymphoid follicles of the nictitating membrane or third eyelid (O'Rourke …

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Variable Patterns Of Distribution Of Prpcwd In The Obex And Cranial Lymphoid Tissues Of Rocky Mountain Elk (Cervus Elaphus Nelsoni) With Subclinical Chronic Wasting Disease, T. R. Spraker, A. Balachandran, D. Zhuang, Katherine I. O'Rourke Jan 2004

Variable Patterns Of Distribution Of Prpcwd In The Obex And Cranial Lymphoid Tissues Of Rocky Mountain Elk (Cervus Elaphus Nelsoni) With Subclinical Chronic Wasting Disease, T. R. Spraker, A. Balachandran, D. Zhuang, Katherine I. O'Rourke

Other Publications in Zoonotics and Wildlife Disease

Sections of medulla oblongata. taken at the level of the obex, palatine tonsil and medial retropharyngeal lymph node from 10,269 captive Rocky Mountain elk (Cervus elaphus nelsoni), were examined by immunohistochemical staining with monoclonal antibody for the prion protein associated with the transmissible spongiform encephalopathy of cervids, chronic wasting disease (PrPCWD). The protein was detected in 226 of them. On the basis of the anatomical location of the deposits in the brainstem of 183 elk, four distinct patterns of distribution of PrPCWD within the parasympathetic region of the dorsal motor nucleus of the vagus nerve …

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