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2003

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Articles 31 - 60 of 318

Full-Text Articles in Veterinary Medicine

Sheep Updates 2003 - Plenaries, Ashley Mercy, David Scobie, Neil Buchanan, Kevin Bell, John Milton, Graeme Martin, Bob Hall, Maurie Stephen, Rob Woodgate Aug 2003

Sheep Updates 2003 - Plenaries, Ashley Mercy, David Scobie, Neil Buchanan, Kevin Bell, John Milton, Graeme Martin, Bob Hall, Maurie Stephen, Rob Woodgate

Sheep Updates

This session covers eight papers from different authors:1. Biosecurity in the sheep industry will improve production and profits Ashley Mercy, Department of Agriculture, Western Australia 2. Easy care sheep David Scobie, AgResearch, PO Box 60 Lincoln, 8152, New Zealand 3. Targeting the market’s requirements - live sheep exports Neil Buchanan, Manager Animal Health Operations, Primary Industries & Resources, South Australia 4. Setting flock breeding objectives Kevin Bell, Sheep Management and Production Consultants, Kojonup WA 5. Future feeding of sheep in Western Australia John Milton and Graeme Martin, The University of Western Australia 6. Recipe for getting back into Sheep Bob …


The Economics Of Managing Wildlife Disease, Richard D. Horan, Christopher A. Wolf Jul 2003

The Economics Of Managing Wildlife Disease, Richard D. Horan, Christopher A. Wolf

Michigan Bovine Tuberculosis Bibliography and Database

The spread of infectious disease among and between wild and domesticated animals has become a major problem worldwide. Upon analyzing the dynamics of wildlife growth and infection when the diseased animals cannot be identified separately from healthy wildlife prior to the kill, we find that harvest-based strategies alone have no impact on disease transmission. Other controls that directly influence disease transmission and/or mortality are required. Next, we analyze the socially optimal management of infectious wildlife. The model is applied to the problem of bovine tuberculosis among Michigan white-tailed deer, with non-selective harvests and supplemental feeding being the control variables. Using …


Magnetic Resonance Images Of The Brain Of A Dwarf Sperm Whale (Kogia Simus), L. Marino, K. Sudheimer, D. A. Pabst, J. I. Johnson Jul 2003

Magnetic Resonance Images Of The Brain Of A Dwarf Sperm Whale (Kogia Simus), L. Marino, K. Sudheimer, D. A. Pabst, J. I. Johnson

Veterinary Science and Medicine Collection

Cetacean (dolphin, whale and porpoise) brains are among the least studied mammalian brains because of the difficulty of collecting and histologically preparing such relatively rare and large specimens. Among cetaceans, there exist relatively few studies of the brain of the dwarf sperm whale (Kogia simus). Magnetic resonance imaging (MRI) offers a means of observing the internal structure of the brain when traditional histological procedures are not practical. Therefore, MRI has become a critical tool in the study of the brain of cetaceans and other large species. This paper represents the first MRI-based anatomically labelled three-dimensional description of the dwarf sperm …


Do Fishes Have Nociceptors? Evidence For The Evolution Of A Vertebrate Sensory System, Lynne U. Sneddon, Victoria A. Braithwaite, Michael J. Gentle Jun 2003

Do Fishes Have Nociceptors? Evidence For The Evolution Of A Vertebrate Sensory System, Lynne U. Sneddon, Victoria A. Braithwaite, Michael J. Gentle

Veterinary Science and Medicine Collection

Nociception is the detection of a noxious tissue-damaging stimulus and is sometimes accompanied by a reflex response such as withdrawal. Pain perception, as distinct from nociception, has been demonstrated in birds and mammals but has not been systematically studied in lower vertebrates. We assessed whether a fish possessed cutaneous nociceptors capable of detecting noxious stimuli and whether its behavior was sufficiently adversely affected by the administration of a noxious stimulus. Electrophysiological recordings from trigeminal nerves identified polymodal nociceptors on the head of the trout with physiological properties similar to those described in higher vertebrates. These receptors responded to mechanical pressure, …


A Genetic Evaluation Of Production Traits For Dairy Cattle In Thailand, Jureeratn Sanpote, Sayan Buaban Jun 2003

A Genetic Evaluation Of Production Traits For Dairy Cattle In Thailand, Jureeratn Sanpote, Sayan Buaban

The Thai Journal of Veterinary Medicine

A study of genetic parameters, breeding values, genetic and phenotypic trends, 305-day milk, fat and protein yields was carried out using 6,357 pedigree and milk records of Holstein Friesian crossbreds, recorded between 1990-2002, in the Master bull project database, Artificial Insemination Division. First lactations, with a lactation length of more than 150 days, were adjusted to a 305-day yield and Restricted, Maximum Likelihood (REML), estimated, variance components. Genetic parameters and breeding values were estimated using the Best Linear Unbiased Prediction (BLUP). The results showed that the average 305-day milk, fat and protein yields were 3,651.87 ± 904.97, 138.32± 39.98 and …


Identification Of Herpes Simplex Virus Type 1 Latency-Associated Transcript Sequences That Both Inhibit Apoptosis And Enhance The Spontaneous Reactivation Phenotype, Ling Jin, Weiping Peng, Guey-Chuen Perng, David J. Brick, Anthony B. Nesburn, Clinton J. Jones, Steven L. Wechsler Jun 2003

Identification Of Herpes Simplex Virus Type 1 Latency-Associated Transcript Sequences That Both Inhibit Apoptosis And Enhance The Spontaneous Reactivation Phenotype, Ling Jin, Weiping Peng, Guey-Chuen Perng, David J. Brick, Anthony B. Nesburn, Clinton J. Jones, Steven L. Wechsler

School of Veterinary and Biomedical Sciences: Faculty Publications

The herpes simplex virus type 1 (HSV-1) latency-associated transcript (LAT) gene is essential for the high spontaneous and induced reactivation phenotype of HSV-1 in the rabbit ocular model and for the high induced reactivation phenotype in the mouse ocular model. Recently we showed that LAT has an antiapoptosis function, and we hypothesized that LAT’s ability to inhibit apoptosis played an important role in LAT’s ability to enhance the reactivation phenotype. Expression of just the first 1.5 kb of the 8.3-kb LAT gene is sufficient for both inhibition of apoptosis in an in vitro transient-transfection assay and the high spontaneous reactivation …


Eimeria Zuernii Infection In Thai Native Calves, Komkrich Teankum, Nopadon Pirarat, Manop Moungyai Jun 2003

Eimeria Zuernii Infection In Thai Native Calves, Komkrich Teankum, Nopadon Pirarat, Manop Moungyai

The Thai Journal of Veterinary Medicine

A 3-month-old, female calf in the Dontan district of Mukdaharn province, showed clinical signs of bloody diarrhoea over a period of 3 days and finally died on April 4, 2001. After necropsy, severe, diffuse, haemorrhagic caeco-colitis and proctitis were markedly evident. Histopathologically, various stages of coccidial development, including schizogony and gametogony, were found in the villous epithelium and the epithelial crypts. Infiltration of lymphocytes, plasma cells, neutrophils and macrophages into the lamina propria, as well as into the submucosa was observed. Another 6-month-old, female calf from a nearby area also showed bloody diarrhoea with mild anorexia and numerous oocysts of …


What Is Your Diagnosis?, Pranee Tuntivanich Jun 2003

What Is Your Diagnosis?, Pranee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


Inactivation Of Picornaviruses And Caliciviruses Part 1: Biology And Epidemiology, Suphachai Nuanualsuwan, Dean O. Cliver Jun 2003

Inactivation Of Picornaviruses And Caliciviruses Part 1: Biology And Epidemiology, Suphachai Nuanualsuwan, Dean O. Cliver

The Thai Journal of Veterinary Medicine

Foodborne picornaviruses and human calicivirus have one molecule of linear, positive-sense, single-stranded RNA, covered by a capsid without a lipid envelope. These viruses have a spherical shape, a particle diameter of 27-40 nanometers and the triangulation (T=3) icosahedral symmetry of a capsid. The replication cycle of poliovirus (PV) and hepatitis A virus (HAV) occurs in the cytoplasm of the host cell. The viral proteins are synthesized by the translation of RNA after the virus particles attach and uncoat at the host cell membrane. Major translated proteins are structural protein (capsid protein) and functional proteins e.g. proteases and RNAdependent RNA polymerase. …


Antimicrobial Susceptibility Tests On Vibrio Pathogens From Black Tiger Shrimps Penaeus Monodon, Nithawan Jarernporn, Cholthida Bantaokul, Nutcharnart Tipmongkolsilp, Monthon Lertworapreecha, Janenuj Wongtavatchai Jun 2003

Antimicrobial Susceptibility Tests On Vibrio Pathogens From Black Tiger Shrimps Penaeus Monodon, Nithawan Jarernporn, Cholthida Bantaokul, Nutcharnart Tipmongkolsilp, Monthon Lertworapreecha, Janenuj Wongtavatchai

The Thai Journal of Veterinary Medicine

The standard, in vitro, minimal inhibitory concentrations (MICs) of 5 antimicrobials; chloramphenicol, ciprofloxacin, enrofloxacin, norfloxacin and nalidixic acid were evaluated against vibrios isolated from diseased black tiger shrimps Penaeus monodon. One hundred and ninety-seven pathogenic vibrio isolates obtained from different shrimp culture areas in Thailand during March 2001-January 2002 were, V. fluvialis (50 isolates, 25.38%), V. damsela (41 isolates, 20.81%), V. vulnificus (39 isolates, 19.80%), V. parahaemolyticus (28 isolates, 14.21%), V. cholerae (25 isolates, 12.69%) and V. alginolyticus (14 isolates, 7.11%). MICs were examined for all the tested antimicrobials and the degree of susceptibility of shrimp vibrio pathogens to each …


Pathological Studies In Thailand On Commercially Valued Fish After Exposure To Crude Cyanotoxin Extract, Piyawat Pholsanong, Achariya Sailasuta, Aparat Mahakhant Jun 2003

Pathological Studies In Thailand On Commercially Valued Fish After Exposure To Crude Cyanotoxin Extract, Piyawat Pholsanong, Achariya Sailasuta, Aparat Mahakhant

The Thai Journal of Veterinary Medicine

Pathological changes in four species of Thai commercially valued fish (age 1-1.5 month), Gunther's walking catfish, Clarius macrocephalus (Gunther), Nile tilapia, Oreochromis niloticus (Linnaeus), Striped catfish, Pangasius hypophthalmus (Sauvage) and common silver barb, Barbodes gonionotus (Bleeker), after exposure to crude cyanotoxin extract, at different concentrations, were investigated. The cyanobacterium, Microcystis aeruginosa, was collected from a fish farm in Pathum Thani province and analyzed using HPLC. Skin, liver and gills were histopathologically examined. The skin showed significant, lesions of irritation and epidermal layers were thickened, by an increase of mucous cells, in the scaled species, Nile tilapia and common silver barb, …


Review Of Molecular Infection Biology: Interactions Between Mircoorganisms And Cells By Jörg Hacker And Jürgen Heesemann, Jeffrey D. Cirillo Jun 2003

Review Of Molecular Infection Biology: Interactions Between Mircoorganisms And Cells By Jörg Hacker And Jürgen Heesemann, Jeffrey D. Cirillo

School of Veterinary and Biomedical Sciences: Faculty Publications

Pathogenesis is one potential outcome of the constant struggle between host defenses and the desire of microorganisms to acquire a privileged niche. This mutually competitive evolutionary process has resulted in interactions among complex and often elegant systems throughout the course of infections. The complexity of these interactions and the large number of infectious agents that exist makes the introduction of students to infectious diseases, as well as a comprehensive review of the field, an extremely daunting task. This book provides just such a comprehensive and timely description of the field in a manner that is easily understandable and enjoyable to …


The Susceptibility Of Thai Native, Mixed Thai Native, Layertype And Broiler Chickens To Infectious Bursal Disease Virus, Thawat Lekdumrongsak, Jiroj Sasipreeyajan Jun 2003

The Susceptibility Of Thai Native, Mixed Thai Native, Layertype And Broiler Chickens To Infectious Bursal Disease Virus, Thawat Lekdumrongsak, Jiroj Sasipreeyajan

The Thai Journal of Veterinary Medicine

Thai native, mixed Thai native and layer-type chickens, 50 birds of each and forty broiler chickens, were reared separately, to determine the susceptibility of each breed to infectious bursal disease virus (IBDV). All birds received IBDV when 36-days-old, given by oral inoculation. Mortality rates were observed for 10 days. Blood samples were collected on days 36, 39, 42 and 46 and sera were tested by an enzyme-linked immunosorbent assay (ELISA) for IBDV antibodies. Bursa to body weight ratios and histopathological lesion scores were determined when the birds were 36 and 46-days-old. The results revealed that mortality rate of Thai native, …


The Use Of Porcine Small Intestinal Submucosa (Psis) In A Canine Deep Corneal Ulcer, Pasakorn Briksawan, Sunti Wongaumnuaykul, Pranee Tuntivanich, Nalinee Tuntivanich, Kumpanart Sunthornvipat Jun 2003

The Use Of Porcine Small Intestinal Submucosa (Psis) In A Canine Deep Corneal Ulcer, Pasakorn Briksawan, Sunti Wongaumnuaykul, Pranee Tuntivanich, Nalinee Tuntivanich, Kumpanart Sunthornvipat

The Thai Journal of Veterinary Medicine

Porcine small intestinal submucosa (PSIS) was used as a graft to repair 13 dogs with deep corneal ulceration. Eight cases had staphyloma and staphyloma associated with hyphema. Two cases had staphyloma associated with glaucoma. There was one case of each of descemetocoele, descemetocoele associated with glaucoma and rupture of the cornea. Ten dogs without glaucoma returned to normal vision (76.93%) with minimal anterior synechia and scarring. Three dogs with associated glaucoma became blind (23.07%). PSIS has been demonstrated to stimulate corneal healing without tissue rejection and is recommended to be used as a graft material to repair deep corneal ulcers …


Cytokine Profiles Following Mycoplasma Hyopneumoniae And Prrsv Co-Infection In Pigs, Roongroje Thanawongnuwech, Eileen L. Thacker Jun 2003

Cytokine Profiles Following Mycoplasma Hyopneumoniae And Prrsv Co-Infection In Pigs, Roongroje Thanawongnuwech, Eileen L. Thacker

The Thai Journal of Veterinary Medicine

Bronchoalveolar cells collected by lung lavage 28 days post infection (DPI) of negative controls, Mycoplasma hyopneumoniae (M. hyo)-infected, Porcine Respiratory and Reproductive Syndrome virus (PRRSV)-infected and dual-infected pigs, were evaluated for various cytokine mRNA (IL1α, IL1β, IL6, IL8, IL10, IL12, and TNFα). Semiquantitative RT-PCR revealed that IL1β, IL6, IL10 and the IL12 mRNA expression was significantly increased in dual-infected pigs, compared with the negative controls. PRRSV-infected pigs had significantly increased levels of IL10 and IL12 mRNA. The increased IL10 gene expression that was observed in the dual infected pigs may play a role in the potentiation of PRRSV-induced pneumonia by …


The Role Of Immune Cells In The Sow Endometrium During Different Stages Of The Oestrous Cycle And Early Pregnancy, Kampon Kaeoket Jun 2003

The Role Of Immune Cells In The Sow Endometrium During Different Stages Of The Oestrous Cycle And Early Pregnancy, Kampon Kaeoket

The Thai Journal of Veterinary Medicine

Approximately 30%(range 13-49%) of sows are culled due to fertility disturbances. Reproductive failure on farms are due to anoestrus, repeat breeding (regular and irregular return to oestrus), negative pregnancy diagnosis, abortion or failure to farrow. Reproductive problems are often difficult to diagnose. As a diagnostic tool, post-mortem examination (i.e. macroscopically and histologically) of the reproductive organs is useful when sows with reproductive failure are culled. The present review provides information about the distribution of immune cells, both in non-inseminated and inseminated pregnant sows endometrium, in order to be able to differentiate a physiological distribution of immune cells from pathological conditions. …


Ecg Quiz, Chollada Buranakarl, Sumpun Thammacharoen Jun 2003

Ecg Quiz, Chollada Buranakarl, Sumpun Thammacharoen

The Thai Journal of Veterinary Medicine

No abstract provided.


The Effects Of Weaning-To-Oestrus Interval On The Expression Of Oestrus And The Timing Of Ovulation In Sows, Nontakorn Urasopon, Wichai Tantasuparuk, Mongkol Techakumphu Jun 2003

The Effects Of Weaning-To-Oestrus Interval On The Expression Of Oestrus And The Timing Of Ovulation In Sows, Nontakorn Urasopon, Wichai Tantasuparuk, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

The aim of the present study was to investigate the effects of the weaning-to-oestrus interval (WOI) and parity on the expression of oestrus and the timing of ovulation in crossbred (Large White x Landrace) sows. The detection of pro-oestrus and oestrus was performed every 8 hrs in all sows, by observing the reddening and swelling of the vulva and by applying the back pressure test in the presence of a boar. Ovulation was monitored every 8 hrs using transrectal ultrasonography. The duration of oestrus was 59.1, 59.8 and 65.3 hrs for parities 1, 2 and 3-4, respectively. Parity 1 and …


Trigeminal Somatosensory Innervation Of The Head Of A Teleost Fish With Particular Reference To Nociception, Lynne U. Sneddon May 2003

Trigeminal Somatosensory Innervation Of The Head Of A Teleost Fish With Particular Reference To Nociception, Lynne U. Sneddon

Veterinary Science and Medicine Collection

Trigeminal somatosensory receptors have not been characterised in teleost fish and studies in elasmobranchs have failed to identify nociceptors. The present study examined the trigeminal nerve of a teleost fish, the rainbow trout (Oncorhynchus mykiss) to determine what types of somatosensory receptors were present on the head of the trout specifically searching for nociceptors. Single unit recordings were made from receptive fields on the head of the fish innervated by the trigeminal nerve. Each receptive field was tested for sensitivity to mechanical, thermal and chemical stimulation. Five different receptor types were found: fast adapting receptors responding to mechanical stimulation; slowly …


Enhancement Of Ehrlichia Canis Infection Of Canine Blood Monocytes In Vitro Through The Addition Of Seropositive And Monoclonal Antibody, John Paul Gniady May 2003

Enhancement Of Ehrlichia Canis Infection Of Canine Blood Monocytes In Vitro Through The Addition Of Seropositive And Monoclonal Antibody, John Paul Gniady

Honors Capstones

No abstract provided.


Addressing The Decline In Reproductive Performance Of Lactating Dairy Cows: A Researcher’S Perspective, William J. Silvia May 2003

Addressing The Decline In Reproductive Performance Of Lactating Dairy Cows: A Researcher’S Perspective, William J. Silvia

Animal and Food Sciences Faculty Publications

No abstract provided.


Infection Of Cattle With A Bovine Herpesvirus 1 Strain That Contains A Mutation In The Latency-Related Gene Leads To Increased Apoptosis In Trigeminal Ganglia During The Transition From Acute Infection To Latency, Luciane Lovato, Melissa Inman, Gail A. Henderson, Alan R. Doster, Clinton J. Jones Apr 2003

Infection Of Cattle With A Bovine Herpesvirus 1 Strain That Contains A Mutation In The Latency-Related Gene Leads To Increased Apoptosis In Trigeminal Ganglia During The Transition From Acute Infection To Latency, Luciane Lovato, Melissa Inman, Gail A. Henderson, Alan R. Doster, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine herpesvirus 1 (BHV-1) is an important pathogen of cattle and infection is usually initiated via the ocular or nasal cavity. After acute infection, the primary site for BHV-1 latency is sensory neurons in the trigeminal ganglia (TG). Reactivation from latency occurs sporadically, resulting in virus shedding and transmission to uninfected cattle. The only abundant viral transcript expressed during latency is the latencyrelated (LR) RNA. An LR mutant was constructed by inserting three stop codons near the beginning of the LR RNA. This mutant grows to wild-type (wt) efficiency in bovine kidney cells and in the nasal cavity of acutely …


The Use Of Real Time B-Mode Ultrasound To Detect Ovulation And Pregnancy In Swine, Kampon Kaeoket Mar 2003

The Use Of Real Time B-Mode Ultrasound To Detect Ovulation And Pregnancy In Swine, Kampon Kaeoket

The Thai Journal of Veterinary Medicine

Real time B-mode ultrasound for ovarian examination in pigs has been used in many experimental studies. All were aimed at elucidating the relationship between the duration of oestrus, the time of ovulation, the time of insemination and fertility. This review describes the uses and benefits of real time B-mode ultrasound for ovulation monitoring and pregnancy diagnosis for fertility control in swine breeding herds. The reliability of diagnosis is very much dependent on the experience of the investigator and the type of ultrasound machine used. It is considered that real time B-mode ultrasound is a useful tool for monitoring the ovulation …


Safety Evaluation Of Trichlorfon Used At A Therapeutic Dose Level For Ectoparasitic Treatment By Studying Serum Cholinesterase Activity Measurements In Carp (Cyprinus Carpio), Rangsiwan Pet-In, Rujira Thumma, Ubonrat Nimittritip, Janenuj Wongtavatchai, Piyarat Chansiripornchai Mar 2003

Safety Evaluation Of Trichlorfon Used At A Therapeutic Dose Level For Ectoparasitic Treatment By Studying Serum Cholinesterase Activity Measurements In Carp (Cyprinus Carpio), Rangsiwan Pet-In, Rujira Thumma, Ubonrat Nimittritip, Janenuj Wongtavatchai, Piyarat Chansiripornchai

The Thai Journal of Veterinary Medicine

The safety of trichlorfon at a therapeutic dose for ectoparasitic treatment in healthy carp (Cyprinus carpio) was evaluated by the measurement of serum cholinesterase (ChE) activity. Three groups of juvenile carp (70-80 g b.w., n =30 each) were bathed with 0.5 ppm trichlorfon 1, 2 or 3 times, respectively, at 5-day intervals. Serum ChE activity in each fish were compared pre and post-treatment. A significant post-treatment reduction of serum ChE activity (p < 0.05) was found in all groups, although they, resumed their pre-treatment levels 14 days after trichlorfon was withdrawn. All fish were clinically healthy throughout the experiment, suggesting, that the doses of trichlorfon applied in this study were safe.


Paralesional Betamethasone Injections For The Treatment Of Kissing-Spines-Syndrome In A Horse: A Case Report, Voraphan Na Songkhla Mar 2003

Paralesional Betamethasone Injections For The Treatment Of Kissing-Spines-Syndrome In A Horse: A Case Report, Voraphan Na Songkhla

The Thai Journal of Veterinary Medicine

A 4-year-old, thoroughbred mare showed clinical signs of back pain and weakness of both hindlimbs. Kissing- Spines-Syndrome (KSS) of the T14-16 dorsal spinous processes were diagnosed from clinical examination and radiography. The horse returned to training after three months and to racing in five months after paralesional betamethasone injections.


What Is Your Diagnosis?, Pranee Tuntivanich Mar 2003

What Is Your Diagnosis?, Pranee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


Antibiotic Susceptibility Of Pigs To E. Coli F4+, Pornchalit Assavacheep, Anongnart Somchit, Apisida Srikrauedong, Nipaporn Paiboonkijkul Mar 2003

Antibiotic Susceptibility Of Pigs To E. Coli F4+, Pornchalit Assavacheep, Anongnart Somchit, Apisida Srikrauedong, Nipaporn Paiboonkijkul

The Thai Journal of Veterinary Medicine

The prevalence and antibiotic sensitivity of E. coli., and haemolytic E. coli. F4+ and haemolytic E. coli. F4- (E/F4+, E/F4-, HE/F4+ and HE/F4-) was investigated in five hundred and thirty, randomly collected, rectal swabs from pig herds in five areas of Thailand, between the dates June 2000 and January 2001. The prevalence of E/F4+, in each area was statistically significantly different with a range of 21.8-42.5% (30.4±8.8%). The greatest prevalence was found in the Northern area of Thailand, following by the Central, Western, Eastern, and Northeastern areas, respectively. The prevalence of E. coli. and haemolytic E. coli. F4+ and F4- …


Dna Fingerprinting Of Leptospira Spp. Using A Repetitive Sequence-Based Polymerase Chain Reaction, Alongkorn Amonsin, Chailai Koowatananukul, Thanis Damrongwatanapokin, Somatat Wongsawang Mar 2003

Dna Fingerprinting Of Leptospira Spp. Using A Repetitive Sequence-Based Polymerase Chain Reaction, Alongkorn Amonsin, Chailai Koowatananukul, Thanis Damrongwatanapokin, Somatat Wongsawang

The Thai Journal of Veterinary Medicine

A DNA fingerprinting technique, repetitive sequence based polymerase chain reaction (rep-PCR), was used to characterize 20 isolates of both pathogenic and nonpathogenic Leptospira spp. Rep-PCR differentiated 20 Leptospira isolates into 16 rep-PCR patterns with a high index of discrimination (D=0.963). The results of cluster analysis demonstrated that pathogenic and nonpathogenic Leptospira belonged to separate clusters with at least 60% similarity. Overall, the results of our studies indicated that rep-PCR is useful for fingerprinting Leptospira species. The technique is simple, rapid, inexpensive and may be used for strain tracking in molecular epidemiological investigations of Leptospirosis outbreaks in the future.


An Inactivated Newcastle Disease Vaccine Part Ii: The Efficacy Of An Inactivated, Experimental Vaccine On The Prevention Of Newcastle Disease, Somsak Pakpinyo, Jiroj Sasipreeyajan, Niwat Chansiripornchai Mar 2003

An Inactivated Newcastle Disease Vaccine Part Ii: The Efficacy Of An Inactivated, Experimental Vaccine On The Prevention Of Newcastle Disease, Somsak Pakpinyo, Jiroj Sasipreeyajan, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

A comparison of the efficacy of a Newcastle disease (ND), inactivated, experimental vaccine (NDIEV) and a ND, inactivated, commercial vaccine (NDICV) was under taken. Broiler chickens were divided into 5 groups of 112 birds each. All groups, except group 5 which served as unvaccinated controls, were given a live ND vaccine, strain B1 and different inactivated ND vaccines as follows. Groups 1 and 3 were given a NDIEV at 1 and 10 days old. And groups 2 and 4 were given a NDICV at 1 and 10 days old. When 21, 28, and 35 days old, 28 birds from each …


Neoplasms Of Dogs In Bangkok, Anudep Rungsipipat, Boonmee Sunyasootcharee, Lek Ousawaphlangchai, Achariya Sailasuta, Roongroje Thanawongnuwech, Komkrich Teankum Mar 2003

Neoplasms Of Dogs In Bangkok, Anudep Rungsipipat, Boonmee Sunyasootcharee, Lek Ousawaphlangchai, Achariya Sailasuta, Roongroje Thanawongnuwech, Komkrich Teankum

The Thai Journal of Veterinary Medicine

2,425 neoplasms from dogs in Bangkok were submitted to the Department of Pathology, Chulalongkorn University, from January 1982 to December 2000 (a 19-year period) and were retrospectively studied to survey the prevalence of the different types of tumours. Histopathological diagnosis was based on the World Health Organization (WHO, 1974) classification of tumours in domestic animals. The prevalence of canine tumours was categorized into skin and soft tissue tumours, 55.05% (1,335 cases); mammary tumours, 28.29% (686 cases); reproductive tumours, 9.69% (235 cases); musculoskeletal tumours, 1.86% (45 cases); digestive tumours, 2.68% (65 cases); hematopoietic and lymphatic tumours, 1.94% (47 cases) and urinary …