Pharmacy and Pharmaceutical Sciences Commons^™

Research News, Grants, 2023, Volume 4, Soumyajit Majumdar

Grants (2015-)

Grants and contracts awarded during October - December 2023

Evaluation Of Selenomethionine Entrapped In Nanoparticles For Oral Supplementation Using In Vitro, Ex Vivo And In Vivo Models, Shane Forde, Giuliana Vozza, David J. Brayden, Jesus Maria Frias, Sinead M. Ryan

Articles

Selenium methionine (Se Met) is an essential micronutrient required for normal body function and is associated with additional health benefits. However, oral administration of Se Met can be challenging due to its purported narrow therapeutic index, low oral bioavailability, and high susceptibility to oxidation. To address these issues, Se Met was entrapped in z e i n-coated nanoparticles made from chitosan using an ionic gelation formulation. The high stability of both the Se Met and s e l e n o methionine nanoparticles (Se Met-NPs) was established using cultured human intestinal and liver epithelial cells, rat liver homogenates, and rat …

Usability Evaluation Of A Community Pharmacy Health Information Exchange Interface Prototype, Katelyn N. Hettinger, Margie E. Snyder, Omolola A. Adeoye-Olatunde, Alissa L. Russ-Jara

Department of Pharmacy Practice Faculty Publications

Objective
Few community pharmacies have access to health information exchange (HIE) data. We conducted a first-of-its-kind usability evaluation of an HIE interface prototype (referred to throughout as the “HIE-Pioneer mock-up”) developed with pharmacists and pharmacy technicians to aid future implementation in community pharmacies.

Methods
Community pharmacists and pharmacy technicians were recruited to complete usability evaluations with the HIE-Pioneer mock-up. Each usability evaluation lasted up to 60 minutes. System usability scale (SUS) scores were collected from each participant following each usability evaluation session and summarized with descriptive statistics. Usability evaluation videos were reviewed for common usability attributes, such as the impact …

Disparities In Medicare Beneficiaries’ Receiving Medication Synchronization, Prajakta H. Waghmare, Chien-Yu Huang, Heather A. Jaynes, Wendy M. Green, Margie E. Snyder, Omolola A. Adeoye-Olatunde, Antoinette B. Coe, Joel F. Farley, Alan Zillich

Department of Pharmacy Practice Faculty Publications

Background

Medication synchronization (med-sync) aligns patients’ monthly or quarterly chronic medications to a predetermined single pickup date at a community pharmacy. The study objective was to examine med-sync enrollment disparities based on Medicare beneficiaries’ predisposing, enabling, and need characteristics.

Methods

This was a retrospective cohort study using a Medicare dataset of beneficiaries receiving medications from pharmacies that self-identified as providing med-sync. Medicare beneficiaries who were continuously enrolled in fee-for-service medical and pharmacy benefits during the study period (2014–2016) were included. Study cohorts (med-sync and non–med-sync patients) were defined, and bivariate and multivariable logistic regression analyses were performed. Andersen’s Health …

Lower Mortality With Andexanet Alfa Vs 4-Factor Prothrombin Complex Concentrate For Factor Xa Inhibitor-Related Major Bleeding In A U.S. Hospital-Based Observational Study, Paul P. Dobesh, Gregory J. Fermann, Mary J. Christoph, Bruce Koch, Eva Lesén, Hungta Chen, Belinda Lovelace, Theresa Dettling, Mark Danese, Julie Ulloa, Sherry Danese, Craig I. Coleman

Journal Articles: Pharmacy Practice

BACKGROUND: Well-designed studies with sufficient sample size comparing andexanet alfa vs 4-factor prothrombin complex concentrate (4F-PCC) in routine clinical practice to evaluate clinical outcomes are limited.

OBJECTIVES: To compare in-hospital mortality in patients hospitalized with rivaroxaban- or apixaban-related major bleeding who were treated with andexanet alfa or 4F-PCC.

METHODS: An observational cohort study (ClinicalTrials.gov identifier: NCT05548777) was conducted using electronic health records between May 2018 and September 2022 from 354 U.S. hospitals. Inclusion criteria were age ≥18 years, inpatient admission with diagnosis code D68.32 (bleeding due to extrinsic anticoagulation), a record of use of the factor Xa inhibitors rivaroxaban or …

Transmission Competence Of B. Burgdorferi Infected Ixodes Ticks And Development Of An Ospc-Based Lyme Disease Vaccine, Samanta Kamalika

Theses and Dissertations (ETD)

Introduction. Lyme disease, otherwise called Lyme borreliosis, is a vector-borne infectious disease caused by the spirochete Borrelia burgdorferi (Bb). Studying the increase in overall Lyme disease incidence requires understanding the role of the vector Ixodes scapularis (I. scapularis) as the agent that carries the spirochete which is important to limit the occurrence of Lyme disease. Once we understand the vector-pathogen response, it also becomes important to determine the remedies for Lyme disease. Lyme disease can be treated with antibiotics if detected early, but the major obstacle lies in the early detection process. Therefore, it is important to investigate alternative preventive …

Modeling The Bidirectional Relationship Between Shared-Patient Physician Networks And Patient Longitudinal Treatment Patterns: Application To Physician Risky-Prescribing, Xin Ran

Dartmouth College Ph.D Dissertations

Risky-prescribing is a pressing public health concern in the United States. Opioids, benzodiazepines, and non-benzodiazepine sedative-hypnotics (sedative-hypnotics) are three commonly-prescribed but potentially risky drug groups, prescribed alone or in combination. Physician shared-patient networks provide a unique perspective in studying physician network characteristics and structures, as well as their association with the delivery of health care. Understanding how physician shared-patient networks are related to their prescribing may inform network-based interventions targeting risky-prescribing, which is yet to be fully studied.

We investigated patient receipt of risky prescriptions and physician risky-prescribing intensity through the scope of shared-patient networks. We used retrospective Medicare insurance …

Physiologically-Based Pharmacokinetic (Pbpk) Modeling For The Preclinical Development Of Spectinamide Antibiotics, Keyur R. Parmar

Theses and Dissertations (ETD)

Despite being an ancient disease caused by Mycobacterium tuberculosis (Mtb) and after decades of research, tuberculosis (TB) still affects millions of people every year worldwide. In 2018, the World Health Organization (WHO) reported that 10 million people developed tuberculosis and 1.5 million died of the disease. With the increase in the multidrug-resistant (MDR) and extensively drug-resistant (XDR) cases, the treatment for TB with the standard first and second-line therapy is becoming increasingly difficult. Therefore, there is an urgent need to find new anti-TB drugs as combination partners of existing and experimental classes of antimicrobial agents to shorten and simplify the …

Industry Update October 2022, Elaine Harris

Articles

Company news Bend Bioscience invests in expansion of drug-delivery capabilities Bend Bioscience (OR, USA) and NovaQuest Private Equity, have announced plans on 11 October 2022 to invest in a new facility in Bend, Oregon. The group will focus on developing new R&D partnerships, and early phase manufacturing and development activities based on proprietary particle engineering-based drug-delivery technologies. This new facility will consist of approximately 20,000 square feet of laboratory and processing areas and it is anticipated that manufacturing and analytical capabilities will operational by the end of Q2 2023. Announcing the investment decision, Dan Dobry, Bend Bioscience’s co-founder said “We …

Retrospective Evaluation Of Dexmedetomidine Dosing In A Cohort Of Neurologically Injured Obese Critically Ill Patients., Harrison He, Sara A Atyia, Keaton S Smetana, Casey C May

Pharmacy Articles

BACKGROUND: Dexmedetomidine is used in neurocritical care units (NCCUs) due to the light, dose-dependent sedation, and anxiolysis provided. It is unknown how to dose dexmedetomidine in obese patients. The primary objective is to assess the ability to achieve the goal Richmond Agitation Sedation Scale (RASS) measurements in obese patients with a neurological injury who are solely on dexmedetomidine before and after an institutional dosing change from actual body weight (ABW) to adjusted body weight (AdjBW).

METHODS: This study included patients admitted to the NCCU with a neurological condition, required dexmedetomidine for at least 8 h as a sole sedative, and …

Role Of Histone Deacetylase (Hdac) In Epithelial To Mesenchymal Transition (Emt) In A Human Cholangiocyte Model Of Ischemic Cholangiopathy, Priyanshi Pragnesh Parikh

Theses and Dissertations

Organ transplants are a vital intervention for many diseases that result in end stage organ failure. Currently, the donation pool is not meeting the demands of the transplant list. Expanding this pool to include donation after cardiac death (DCD) is highly sought-after. However, the use of DCD livers can lead to increased odds of graft failure and ischemic cholangiopathy. The loss of epithelialization and fibrosis that occurs during ischemic cholangiopathy is characteristic of these cells undergoing epithelial-to-mesenchymal transition (EMT). The biological changes the cell experiences enhance migratory capacity, invasiveness, and increased resistance to apoptosis. Our earlier studies have shown differential …

The Impact Of Hiv-1 Tat And Morphine On Spatial Distribution Of Drugs In The Brain., Austin M. Jones

Theses and Dissertations

Despite combination antiretroviral therapy effectively suppressing HIV within the periphery, the central nervous system (CNS) remains affected by the virus. Approximately half of people living with HIV will experience HIV-associated neurocognitive disorders (HAND) or neuroHIV. Concurrent opioid use exacerbates neuroHIV by promoting neuroinflammation, viral replication, and potentially altering the antiretroviral concentrations within the brain.

Using a transgenic mouse that expresses the HIV-1 Tat protein, we examined the effects of Tat and morphine on antiretroviral accumulation and distribution and the effects of Tat on morphine accumulation within the brain using infrared matrix-assisted laser desorption electrospray ionization with mass spectrometry imaging (IR-MALDESI-MSI). …

Development Of A Chemical Biology Approach To Uncover The Influence Of Sequence Variations On Ces1 Activity In Live Cells, Samuel James Knebel

Masters Theses

Drug metabolism is the biochemical process of modifying drugs to detoxify and remove them through enzymatic transformations. These biotransformation’s occur primarily in the liver and are critical to understanding how pharmaceutical compounds are chemically altered inside the human body. Human carboxylesterases (CESs) catalyze the hydrolysis of esters, amides, thioesters, and carbamates. CES-mediated hydrolysis plays an important role in the metabolism of many drugs including the first FDA approved antiviral treatment for COVID-19, remdesivir (Veklury), the seizure control medication rufinamide (Banzel), and the flu antiviral drug oseltamivir (Tamiflu). CES activity is known to be influenced by a variety of factors including …

Modified Tlr7/8 Adjuvanted Liposomes For Improved Intranasal Delivery Of Subunit Vaccines, Elizabeth Brander Lorentz

Graduate Student Theses, Dissertations, & Professional Papers

Intranasal vaccines hold enormous potential for improving vaccine efficacy against respiratory pathogens by activating the mucosal immune system. The urgency for novel approaches in vaccine development is amplified by the recent COVID-19 pandemic. Most intranasal vaccines that were developed are either live-attenuated or inactivated vaccine candidates that demonstrated poor safety profiles. Protein-based subunit vaccines represent a safe and cost-effective alternative, but they are often weak immunogenically. TLR7/8 adjuvants such as INI-4001 have shown promise for enhancing the humoral and cellular immunogenicity of protein-based subunit vaccines. It is theorized that poor immunogenicity of intranasal protein subunit vaccines is due to low …

Personal Hardiness As An Unexamined Component Of The Healthy Volunteer Effect, Angelique E. Blann

Walden Dissertations and Doctoral Studies

Clinical trials are the standard for approving medicines for public use. To conduct trials, researchers depend on a declining number of volunteers. The healthy volunteer effect, a phenomenon in which participants demonstrate better outcomes than their peers, limits the generalizability of trial outcomes. The healthy volunteer effect does not fully explain the outcome difference between volunteers and nonvolunteers. The purpose of this cross-sectional quantitative study was to examine personal hardiness as an unexplored component of the healthy volunteer effect and determine whether personal hardiness is associated with willingness to participate. Hardiness was a personality construct that represented resilience to stress. …

Personal Hardiness As An Unexamined Component Of The Healthy Volunteer Effect, Angelique E. Blann

Walden Dissertations and Doctoral Studies

Clinical trials are the standard for approving medicines for public use. To conduct trials, researchers depend on a declining number of volunteers. The healthy volunteer effect, a phenomenon in which participants demonstrate better outcomes than their peers, limits the generalizability of trial outcomes. The healthy volunteer effect does not fully explain the outcome difference between volunteers and nonvolunteers. The purpose of this cross-sectional quantitative study was to examine personal hardiness as an unexplored component of the healthy volunteer effect and determine whether personal hardiness is associated with willingness to participate. Hardiness was a personality construct that represented resilience to stress. …

Pharmacy Manager System Implementation Strategies To Mitigate The Cost Of Prescription Errors, Tunde Abiodun Cardozo

Walden Dissertations and Doctoral Studies

AbstractOne of the most frequent medical errors in contemporary medicine is incorrect prescriptions, and the profits from retail pharmacy operations are adversely impacted by the costs associated with prescription errors. Independent pharmacy managers are interested in finding workable strategies to mitigate the cost of prescription errors and increase profit. Using the resource-based theory of competitive advantage (RBTCA), the purpose of this qualitative multiple-case study was to explore strategies some independent pharmacy managers in Texas use to mitigate the cost of pharmacy employee prescription errors and increase profitability. The participants were five independent pharmacy managers who implemented strategies to mitigate the …

Role Of Cysteine Redox State In Altering The Epithelial-Mesenchymal Transition In Lung Cancer, Md Anisur Rahman

University of the Pacific Theses and Dissertations

Epithelial-mesenchymal transition is a process by which cancer cells increase their capability for metastasis. EMT involves conversion of epithelial cells into a mesenchymal phenotype which makes cancer cells more invasive and motile. Lung cancer has the highest mortality rate among different types of cancers globally. Lung cancer exists in various types, the most prevalent of which is non-small cell lung cancer (NSCLC). Several factors, including oxidative stress, can induce EMT in cancer cells. However, the mechanism of how reactive oxygen species (ROS)-induced changes in cysteine redox state affect EMT remains elusive. Our studies aim to understand this mechanism by inducing …

Investigating Bzip Recognition Of Dna Sequences Through A Knob-Socket Perspective, Aaron Tran

University of the Pacific Theses and Dissertations

To investigate whether higher order packing interactions confer protein-DNA specificity, a modified Knob-Socket (KS) model was used to analyze the interface of bZIP-DNA crystal structures. The KS analysis identified a nine-residue quadripartite recognition core consisting of four contiguous KS pockets P1, P2, N3, and N4 that each pack one of the four DNA half-site bases in the target sequence. Only one base per base pair packs, and these interactions are split across the DNA strands: the first two positive strand positions 1p and 2p pack into P1 and P2 while the last two negative strand positions 3n and 4n pack …

Evaluation Of Human Stem Cell-Derived Neurons In 2d And 3d Cultures For Neurodevelopmental Studies, Epilepsy Disease Modeling And Drug Discovery, Hamed Salmanzadeh-Dozdabi

University of the Pacific Theses and Dissertations

Human stem cells have revolutionized the study of early neural development, offering a powerful tool to investigate the complex processes involved in the formation of the nervous system. In this study, the human pluripotent stem cell line, TERA2.cl.SP12, was utilized to derive neuroglial cells and to determine their differentiation and functional maturation up to one year in culture; this model was then investigated as a new tool for the study of neurodevelopmental toxicity, for seizure modeling and as a screening method for the investigation of new anticonvulsants. Immunocytochemistry experiments determined that non-differentiated cells expressed the stem cell marker Oct-3/4 and, …

Targeting Ribonucleotide Reductase For Breast Cancer Treatment, Nahid Sultana

University of the Pacific Theses and Dissertations

Breast cancer is the second most common type of cancer in the world. Hormone receptor (HR) positive breast cancer (BC) is a prevalent disease accounting for approximately 2 million new cases globally. Almost 70-80% of breast cancer patients are women with a positive score for the estrogen receptor (ER). Triple-negative breast cancer (TNBC) which have a negative score for estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor type 2 (HER2) is considered an aggressive histological breast cancer subtype with limited treatment options.Frequently, doxorubicin (DOXO)-based chemotherapy is utilized in this patient population due to the lack of …

Testing The Efficacy And Synergistic Components Of Sesamol And Black Rice Extract On Human Colon Cancer Cells, Sera Lim, Philip M. Gerk

UROP Posters

Purpose: Systemic treatment of colorectal cancer involves chemotherapeutic agents which elicit serious and negative side effects from the toxicity of the drug. To address this issue, we are testing dietary supplements for their efficacy against human colon cancer cell lines and also their potential synergistic effects when combined with conventional chemotherapy. Dietary supplements (specifically sesamol and black rice extract) exhibit anticancer, anti-inflammatory, and chemo-preventive properties. Meanwhile, one of the cancer resistance mechanisms is the upregulation of drug elimination mechanisms, leading to multi-drug resistance. We hypothesize that dietary compounds will act as chemo-enhancers, thus enhancing potency of the chemotherapy drug(s) on …

Lysosomes, Curcumin, And Anti-Tumor Effects: How Are They Linked?, Qian Shen, Xue Pan, Yi Li, Junchen Li, Chuanlong Zhang, Xiaochen Jiang, Fudong Liu, Bo Pang

Faculty, Staff and Student Publications

Curcumin is a natural active ingredient from traditional Chinese medicine (TCM) that has multi-target characteristics to exert extensive pharmacological activities and thus has been applied in the treatment of various diseases such as cancer, cardiovascular diseases, nervous system, and autoimmune disorders. As an important class of membranous organelles in the intracellular membrane system, lysosomes are involved in biological processes such as programmed cell death, cell metabolism, and immune regulation, thus affecting tumor initiation and progression. It has been shown that curcumin can modulate lysosomal function through the aforementioned pathways, thereby affecting tumor proliferation, invasion, metastasis, drug resistance, and immune function. …

ผลลัพธ์ของการตรวจคัดกรองยีนแพ้ยา Hla-B*58:01ก่อนเริ่มใช้ยาอัลโลพูรินอลครั้งแรก ณ โรงพยาบาลระดับตติยภูมิ, พัชรพล เฟื่องประยูร

Chulalongkorn University Theses and Dissertations (Chula ETD)

ที่มาและความสำคัญ: การตรวจยีนแพ้ยา HLA-B*58:01 ก่อนเริ่มใช้ยา allopurinol สามารถช่วยป้องกันการแพ้ยาทางผิวหนังจากการใช้ยา allopurinol ได้ วัตถุประสงค์: เพื่อเปรียบเทียบอุบัติการณ์การแพ้ยาทางผิวหนังจากยา allopurinol โดยรวมทั้งชนิดที่ไม่รุนแรงและรุนแรง และศึกษาความแตกต่างของค่าใช้จ่ายทางตรงที่เกี่ยวข้องกับการรักษาเมื่อพบการแพ้ยาในกลุ่มผู้ป่วยที่ได้รับการตรวจยีนแพ้ยา (กลุ่มศึกษา) และผู้ป่วยที่ไม่ได้รับการตรวจยีนแพ้ยา (กลุ่มเปรียบเทียบ) วิธีการศึกษา: การวิจัยจากเหตุไปหาผลแบบย้อนหลัง ผู้ป่วยที่เข้ารับการรักษาในโรงพยาบาลเจริญกรุงประชารักษ์ กรุงเทพมหานคร ระหว่างวันที่ 1 มกราคม พ.ศ. 2562 ถึงวันที่ 31 มีนาคม พ.ศ. 2567 ผู้ป่วยที่ผ่านคุณสมบัติตามที่ผู้วิจัยกำหนดทั้งหมด 1,026 ราย แบ่งเป็นกลุ่มศึกษา 281 รายและกลุ่มเปรียบเทียบ 745 ราย ผลการศึกษา: ผู้ป่วยกลุ่มศึกษา 281 ราย ตรวจพบยีนแพ้ยาเป็นบวก 53 ราย (ร้อยละ 18.8) ได้รับการรักษาด้วยยาลดกรดยูริกในเลือดอื่น ๆ 32 ราย ตรวจพบยีนแพ้ยาเป็นลบ 228 ราย (ร้อยละ 81.14) ได้รับการรักษาด้วยยา allopurinol 163 ราย และกลุ่มเปรียบเทียบ 745 ราย ผู้ป่วยทุกรายได้รับการรักษาด้วยยา allopurinol ที่ระยะเวลาการติดตามการแพ้ยา 90 วัน ในกลุ่มศึกษาพบอุบัติการณ์การแพ้ยาทางผิวหนังโดยรวม และชนิดที่ไม่รุนแรงเท่ากันร้อยละ 1.4 และกลุ่มเปรียบเทียบร้อยละ 5.9 และ 5.0 ตามลำดับ อย่างมีนัยสำคัญทางสถิติ (p = 0.007, 0.02 ตามลำดับ) และในกลุ่มศึกษาไม่พบอุบัติการณ์การแพ้ยาทางผิวหนังชนิดที่รุนแรง แต่กลุ่มเปรียบเทียบพบร้อยละ 0.9 (p = 0.216) ความแตกต่างของค่าใช้จ่ายทางตรงที่เกี่ยวข้องกับการรักษาเมื่อพบการแพ้ยาระหว่างกลุ่มศึกษาน้อยกว่ากลุ่มเปรียบเทียบ 78,198.52 บาทต่อราย สรุปผลการศึกษา: การตรวจคัดกรองยีน HLA-B*58:01 ก่อนเริ่มใช้ยา allopurinol มีประสิทธิผลในการลดอุบัติการณ์แพ้ยาทางผิวหนัง และลดค่าใช้จ่ายทางตรงที่เกี่ยวข้องกับการแพ้ยา

Synthetic Naphthoquinone Analogs As Pi3k/Akt/M Tor Targeted Inhibitor, Adzkia Fasha

Chulalongkorn University Theses and Dissertations (Chula ETD)

Lung, breast, and colorectal cancers significantly contribute to the high incidence and mortality rates of cancer worldwide. Naphthoquinone is a well-known anticancer agent, including lawsone and napabucasin. The series of lawsone (bicyclic system) and napabucasin (tricyclic system) were designed to find the suitable target proteins though computational modelling, predicting the interactions between 172 naphthoquinone analogues and 12 potential cancer-related target proteins. The virtual study proposed the naphthoquinone analogues based on their superior docking scores, interactions, and absorption, distribution, metabolism, and elimination (ADME) predictions compared to lawsone and napabucasin. Interestingly, the PI3K/AKT/mTOR proteins emerged as the promising target for the naphthoquinones. …

A Scoping Review Of Disease Modeling Approaches In Estimating Snakebite Burden, Ahmed Adamu Gololo

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: Epidemiological modeling studies in snakebite envenoming research are evolving. Their techniques can be essential in filling the knowledge gap needed to attain the World Health Organization’s goal of halving the burden of snakebite envenoming by complementing the current data scarcity. Hence, there is a need for a scoping review to summarize epidemiological models used in estimating the burden of snakebite envenoming. Methods: We conducted a scoping review by searching PubMed, EMBASE, and Scopus to identify articles reporting epidemiological models in snakebite envenoming from database inception to 31st December 2023. The study protocol was registered with Open Science Framework (https://osf.io/u6cyh/). …

The Development Of Weight Management Model “Weightcut” For Community Pharmacists’ Practice, Pairin Supsongserm

Chulalongkorn University Theses and Dissertations (Chula ETD)

Obesity is one of the health problems that could cause health and economic and social impacts. However, few community pharmacists provided weight management services (WMS) and there has not been standard practice for weight management by community pharmacists in Thailand. The objectives of this study were: 1) to systematically review factors affecting community pharmacists' intention and weight management service provision in various countries; 2) to explore factors affecting community pharmacists' intention and weight management service provision in Thailand by using survey; and 3) to develop weight management service called “WeightCut” for community pharmacy practice in Thailand. From systematic review, lack …

Protective Effect Of Naringenin Against Indomethacin-Induced Lysosomal Toxicity, Kaitsuda Saiprom

Chulalongkorn University Theses and Dissertations (Chula ETD)

An increase in lysosomal membrane permeabilization (LMP) impaired lysosome integrity causing release of cathepsin B, and subsequently may contribute to cell death. Indomethacin, which is a non-steroidal anti-inflammatory drugs (NSAIDs), caused apoptosis through generation of cell stress, and disruption of mitochondria and lysosome functions. Recently, it was reported that naringenin, a natural bioactive flavonoid, elicited cytoprotection against indomethacin toxicity in Caco-2 cells. This study investigated the effect of naringenin on indomethacin-induced lysosome toxicity in human colon carcinoma Caco-2 cells. Cells were pretreated with naringenin (100 µM) for 24 h before incubating with indomethacin for another 48-72 h. At the end …

Determination Of Valsartan Enantiomers By Ligand-Exchange Chromatography Using Chiral Mobile Phase Additives, Chanbopha Tho

Chulalongkorn University Theses and Dissertations (Chula ETD)

A simple, accurate, and low-cost effective method has been introduced in this study for separation of valsartan enantiomers using ligand-exchange chromatography as chiral mobile phase additives. The optimized method was found using a mobile phase system of 57%(v/v) methanol, adding 1.5 mM of L-isoleucine as the chiral selector along with 0.5 mM of cupric sulfate and 10 mM of sodium acetate, adjusted to pH 6.1. The flow rate was 0.8 mL/min with a temperature of 400C. The injection volume was 10 µL and detected at 210 nm. The stationary phase was a reversed-phase C18 Zorbax Eclipse Plus (250 mm x …

Development Of Plant-Produced Proteins For Treatment And Prevention Against Rsv Type A (Rsv-A), Nuttapat Pisuttinusart

Chulalongkorn University Theses and Dissertations (Chula ETD)

Respiratory Syncytial Virus (RSV), a highly contagious virus known to cause severe respiratory illnesses, poses a significant health threat, particularly impacting vulnerable populations such as young children, the elderly, and immunocompromised individuals. It is a leading cause of lower respiratory tract infections, often resulting in serious complications like pneumonia and bronchiolitis. Despite its substantial impact, there is currently only an approved RSV vaccine specifically designed for the elderly, emphasizing the urgency of continued research in this area for developing broader coverage of the population. The plant expression platform for producing therapeutic proteins offers a cost-effective and scalable solution, presenting a …