Oncology Commons^™

A Stable Explant Culture Of Her2/Neu Invasive Carcinoma Supported By Alpha-Smooth Muscle Actin Expressing Stromal Cells To Evaluate Therapeutic Agents, Marie P. Piechocki

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

To gain a better understanding of the effects of therapeutic agents on the tumor microenvironment in invasive cancers, we developed a co-culture model from an invasive lobular carcinoma. Tumor cells expressing HER2/neu organize in nests surrounded by alpha-Smooth Muscle Actin (α-SMA) expressing tumor stroma to resemble the morphology of an invading tumor. This co-culture, Mammary Adenocarcinoma Model (MAM-1) maintains a 1:1 ratio of HER2/neu positive tumor cells to α-SMA-reactive stromal cells and renews this configuration for over 20 passages in vitro.

Methods

We characterized the cellular elements of the MAM-1 model by microarray analysis, and immunocytochemistry. We developed …

Preclinical Studies Of Apogossypolone: A New Nonpeptidic Pan Small-Molecule Inhibitor Of Bcl-2, Bcl-XL And Mcl-1 Proteins In Follicular Small Cleaved Cell Lymphoma Model, Alan A. Arnold, Amro Aboukameel, Jianyong Chen, Dajun Yang, Shaomeng Wang, Ayad Al-Katib, Ramzi M. Mohammad

Wayne State University Associated BioMed Central Scholarship

Abstract

Elevated expression of anti-apoptotic Bcl-2 family proteins have been linked to a poor survival rate of patients with Follicular Lymphoma (FL). This prompted us to evaluate a very potent non-peptidic Small-Molecule Inhibitor (SMI) targeting Bcl-2 family proteins, Apogossypolone (ApoG2) using follicular small cleaved cell lymphoma cell line (WSU-FSCCL) and cell isolated from lymphoma patients. ApoG2 inhibited the growth of WSU-FSCCL significantly with a 50% growth inhibition of cells (IC₅₀) of 109 nM and decreased cell number of fresh lymphoma cells. ApoG2 activated caspases-9, -3, and -8, and the cleavage of Poly (ADP-ribose) polymerase (PARP) and Apoptosis Inducing …

The Function And Mechanism Of Chmp1a In Tumor Development, Jing Li

Theses, Dissertations and Capstones

Chmp1A (Chromatin modifying protein 1A/Charged multivesicular protein 1A) is a member of the ESCRT-III (Endosomal Sorting Complex Required for Transport) family, which mediates trafficking via MVB (multivesicular body) formation and sorting. Our studies suggest that Chmp1A is a novel tumor suppressor, especially in the pancreas. Knockdown of Chmp1A resulted in an increase of anchorage-independent growth of HEK 293T cells. Moreover, we showed that Chmp1A depleted HEK 293T cells forms tumor in xenograft mice. Knockdown of Chmp1A in PanC-1 cells promoted cell growth. In contrast, Doxycycline induced over-expression of Chmp1A in pancreatic cancer cells (PanC-1) resulted in cell growth inhibition, tumor …

Robotic Rectal Cancer Surgery, Minia Hellan, Joshua Ellenhorn, Alessio Pigazzi

Department of Surgery Faculty Publications

No abstract provided.

Beliefs, Perceptions, And Preferences For Treatment In Latinas With Breast Cancer, Barbara Ann Kreling

Walden Dissertations and Doctoral Studies

Research documents that breast cancer is the leading cause of death in Latina females. The exact numbers are unknown, but studies reveal that Latinas with breast cancer underuse recommended follow-up chemotherapy, decreasing their rates of survival. Although several factors may be responsible, cultural influences are a possible barrier. However, there is a gap in the literature about how culture affects decisions about breast cancer treatment. This focused ethnographic study examined the role of cultural beliefs and perceptions in the decision-making process for Latina women about whether or not to receive chemotherapy following a breast cancer diagnosis. Drawing from Douglas' cultural …

Living Past Your Expiration Date: A Phenomenological Study Of Living With Stage Iv Cancer Longer Than Expected, Cynthia Levine

Antioch University Dissertations & Theses

More treatment options exist today for persons diagnosed with terminal cancerextending lives longer than expected though there is little known about the psychosocial needs or resources for these individuals. This study describes the experience of living past the expiration date and still living with Stage IV cancer. A transcendental phenomenological approach was used to elucidate vivid expressions of this experience in a sample population of five Caucasian women. The women survived beyond their prognoses of an earlier expiration are not close to imminent death and are still living with incurable breast cancer metastases. The aim of this phenomenological inquiry is …

Oncolog Volume 53, Number 10, October 2008, Joe Munch, Sunita Patterson

OncoLog MD Anderson's Report to Physicians (All issues)

• Pancreatic Cancer: The Prognosis Is Changing
• Delivering “The Guardian of the Genome”
• House Call: “Seeing” Cancer from the Outside
• Catching Pancreatic Cancer Early

Oncolog Volume 53, Number 09, September 2008, Maude Veech, Dawn Chalaire

OncoLog MD Anderson's Report to Physicians (All issues)

• Deciphering Metastatic Colorectal Carcinoma
• House Call: Planning Ahead with Advance Directives
• A Child-Centered Approach to Anesthesia for Proton Therapy

Oncolog Volume 53, Number 02, February 2008, Joe Munch, Diane Witter, Guillermo Garcia-Manero Md

OncoLog MD Anderson's Report to Physicians (All issues)

• Assessing Symptom Burden in Cancer
• DiaLog: Making Headway in Hematology, by Guillermo Garcia-Manero, MD, Associate Professor, Department of Leukemia
• Helping Kids Make Smart Food Choices
• Expanding Therapy Options for Advanced Cancer

Oncolog Volume 51, Number 01, January 2006, Stephanie P. Deming, Diane Witter

OncoLog MD Anderson's Report to Physicians (All issues)

• Fertility After Cancer
• Improving the Odds in Lung Cancer
• House Call: Understanding the Basics of Melanoma

Oncolog Volume 53, Number 06, June 2008, Karen Stuyck, Virginia M. Mohlere, David Stouter, David Jenkins

OncoLog MD Anderson's Report to Physicians (All issues)

• Psychotherapy Extends Cancer Care Beyond the Physical
• House Call: Understanding How Cancer Spreads
• DiaLog: Teaming Spiritual and Medical Care, by Rev. David Stouter, Chaplain, Department of Chaplaincy and Pastoral Education, and Rev. David Jenkins, Director, Department of Chaplaincy and Pastoral Education
• Radical Trachelectomy: A Fertility-Sparing Option for Invasive Cervical Cancer

Oncolog Volume 53, Number 05, May 2008, John Lebas, Joe Munch, Alexander V. Prokhorov Md, Phd

OncoLog MD Anderson's Report to Physicians (All issues)

• Hepatocellular Carcinoma: New Standards in Therapy Emerging
• House Call: When Diagnosis Meets Denial
• In Brief: Overweight, Obese Patients Fare Worse with Breast Cancer, Researchers Find
• DiaLog: ASPIRE to Stop Teen Smoking, by Alexander V. Prokhorov, MD, PhD, Project Director, ASPIRE, Professor, Department of Behavioral Science
• A Reverse Approach to Adrenal Gland Resection

Oncolog Volume 53, Number 03, March 2008, John Lebas, Gordon B. Mills Md, Phd

OncoLog MD Anderson's Report to Physicians (All issues)

• In Search of the Answer
• DiaLog: Mapping the Cancer Genome, by Gordon Mills, MD, PhD, Director, Kleberg Center for Molecular Markers, Chair, Department of Systems Biology
• House Call: The Basics of Biopsies

2007 Cancer Care Annual Report, Children's Mercy Hospital

Cancer Center Annual Reports

Annual report of cancer care and oncology services at the Children's Mercy Hospital, Kansas City MO, a pediatric medical center.

Prolonged Hemophagocytic Lymphohistiocytosis Syndrome As An Initial Presentation Of Hodgkin Lymphoma: A Case Report., Kathryn Chan, Eric Behling, David S Strayer, William S Kocher, Scott K Dessain

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

INTRODUCTION: Hemophagocytic lymphohistiocytosis is an immune-mediated syndrome that typically has a rapidly progressive course that can result in pancytopenia, coagulopathy, multi-system organ failure and death. CASE PRESENTATION: A 57-year-old Caucasian woman was referred in fulminant hemophagocytic lymphohistiocytosis, with fever, pancytopenia, splenomegaly, mental status changes and respiratory failure. She was found to have stage IV classical Hodgkin lymphoma, in addition to Epstein-Barr virus and cytomegalovirus viremia. Her presentation was preceded by a 3-year prodrome consisting of cytopenia and fever that were partially controlled by steroids and azathioprine. CONCLUSION: Fulminant hemophagocytic lymphohistiocytosis may follow a prodromal phase that possesses features suggestive of …

Distinguishing Post-Treatment Changes From Recurrent Disease In Cholangiocarcinoma: A Case Report., Timothy N Showalter, A Omer Nawaz, Frederick M Fellin, Pramila R Anne, Ernest L Rosato, Adam P Dicker

Department of Radiation Oncology Faculty Papers

INTRODUCTION: Three-dimensional techniques for radiotherapy have expanded possibilities for partial volume liver radiotherapy. Characteristic, transient radiographic changes can occur in the absence of clinical radiation-induced liver disease after hepatic radiotherapy and must be distinguished from local recurrence. CASE PRESENTATION: In this report, we describe computed tomography changes after chemoradiotherapy for cholangiocarcinoma as an example of collaboration to determine the clinical significance of the radiographic finding. CONCLUSION: Because of improved three-dimensional, conformal radiotherapy techniques, consultation across disciplines may be necessary to interpret post-treatment imaging findings.

Hiv-1 Tat And Aids-Associated Cancer: Targeting The Cellular Anti-Cancer Barrier?, Giuseppe Nunnari, Johanna A Smith, René Daniel

Department of Medicine Faculty Papers

The acquired immunodeficiency syndrome (AIDS) is accompanied by a significant increase in the incidence of neoplasms. Several causative agents have been proposed for this phenomenon. These include immunodeficiency and oncogenic DNA viruses and the HIV-1 protein Tat. Cancer in general is closely linked to genomic instability and DNA repair mechanisms. The latter maintains genomic stability and serves as a cellular anti-cancer barrier. Defects in DNA repair pathway are associated with carcinogenesis. This review focuses on newly discovered connections of the HIV-1 protein Tat, as well as cellular co-factors of Tat, to double-strand break DNA repair. We propose that the Tat-induced …

Oncolog Volume 53, Number 11, November 2008, John Lebas, Sunni Hosemann

OncoLog MD Anderson's Report to Physicians (All issues)

• Options for Malignant Pleural Mesothelioma
• House Call: Saying No to Smoking: When You Need More than Willpower
• Intermediate-Stage Bladder Cancer: Deciding on Preoperative Chemotherapy and Urinary Diversion

Oncolog Volume 53, Number 04, April 2008, Diane Witter, John Lebas, Sunni Hosemann

OncoLog MD Anderson's Report to Physicians (All issues)

• Cancer as a Chronic Disease
• In Brief: Cancer-Promoting Protein in Ovarian Cancer May Be Stopped with RNA Liposome
• House Call: Caring for the Caregiver
• Barely Benign

Oncolog Volume 53, Number 01, January 2008, John Lebas, Don Norwood

OncoLog MD Anderson's Report to Physicians (All issues)

• Prostate Cancer: A Tale of Two Therapies
• In Brief: Newly Identified Gene May Stop Development of Lung Cancers
• House Call: What You Should Know About Male Cancers
• Making Headway Against Myeloproliferative Disorders

Oncolog Volume 53, Number 07/08, July/August 2008, John Lebas, Sunni Hosemann

OncoLog MD Anderson's Report to Physicians (All issues)

• Multiple Myeloma: Increasing Response, Remission, and Survival Rates in a Complex Disease
• House Call: A Guide to Radiation Therapy
• In Brief: Researchers Enhance Anticancer Activity of Natural Killer Cells in Laboratory
• Beyond Images: Advances in Interventional Radiology
• Radioembolotherapy Using Yttrium 90

Maldi Mass Spectrometry Imaging For The Discovery Of Prostate Carcinoma Biomarkers, Lisa Harris Cazares

Theses and Dissertations in Biomedical Sciences

The elucidation of new biological markers of prostate cancer (PCa) should aid in the detection, and prognosis of this disease. Diagnostic decision making by pathologists in prostate cancer is highly dependent on tissue morphology. The ability to localize disease-specific molecular changes in tissue would help improve this critical pathology decision making process. Direct profiling of proteins in tissue sections using MALDI imaging mass spectrometry (MALDI-IMS) has the power to link molecular detail to morphological and pathological changes, enhancing the ability to identify candidates for new specific biomarkers. However, critical questions remain regarding the integration of this technique with clinical decision …

Dosimetric Evaluation Of Helical Tomotherapy Treatment Planning For Non-Small Cell Lung Cancer, Karen Chu, George Rodrigues, Slav Yartsev, A. Rashid Dar, Edward Yu, Robert Ash, Brian Yaremko, Marc Mackenzie, Harvey Quon, Glenn Bauman, Wilson Roa

Edward Yu

Helical tomotherapy (HT) is a novel technique to deliver intensity modulated radiation therapy guided by 3D megavoltage CT imaging. The purpose of our study is to assess the dosimetric parameters related to HT and 3DCRT in advanced non-small cell lung cancer (NSCLC). Eleven patients from the London Regional Cancer Centre and the Cross Cancer Institute with NSCLC underwent individualized treatment planning on both HT and 3DCRT. Corresponding HT and 3DCRT plans for each patient were analyzed using dose-volume histograms for GTV, PTV (median dose 60Gy/30 fractions), and critical structures (lung V5-30, esophageal V50-60, and spinal cord D1). Observed differences in …