Medicine and Health Sciences Commons^™

Sex Differences In Peripheral Fat Detection And Metabolism, Naima Dahir

Electronic Theses and Dissertations, 2020-2023

The current epidemic of obesity is driven, at least in part, by excessive caloric intake and fat intake has been implicated as a major contributing factor. The mammalian taste system displays marked plasticity, and it has been shown that the detection of fatty acids, the prototypical fat taste stimulus, leads to reduced sensitivity upon prolonged exposure to a high-fat diet. Increased obesity and metabolic disorders in postmenopausal females indicate that the female steroid hormone, estrogen, may contribute to metabolic homeostasis. Sex differences in the taste system were explored using a multidisciplinary approach to examine the ways in which estrogen influences …

Anti-Map Triple Therapy Supports Immunomodulatory Therapeutic Response In Crohn's Disease Through Downregulation Of Nf-Kb Activation In The Absence Of Map Detection, Erij Elkamel

Electronic Theses and Dissertations, 2020-2023

The triple antibiotic formulation, known as anti-MAP therapy, exhibits unique synergistic antimicrobial activity and should be effective for treatment of Crohn's disease (CD) associated with Mycobacterium avium subspecies paratuberculosis (MAP). The absence of MAP detection in some CD cases may be linked to poor diagnostics or lack of association with the disease. To understand the therapeutic response of some CD patients to anti-MAP therapy in absence of MAP detection, the immunomodulatory potency of anti-MAP therapy and its major ingredients, clarithromycin (CLA) and rifabutin (RIF), in THP-1, Caco-2, and Jurkat T-cells were investigated. Anti-MAP formulation at 2.0 µg/mL decreased MAP viability …

Knowledge And Attitudes Of Nurse Practitioner's Assessment Of Adults With Signs And Symptoms Of Posttraumatic Stress Disorder, Kelli Lipscomb

Electronic Theses and Dissertations, 2020-2023

As the numbers of primary care physicians decline, nurse practitioners (NPs) are filling this gap as primary care providers in many settings. The number of adults presenting to their primary care provider with signs and symptoms of posttraumatic stress disorder (PTSD) each year is unknown. The devastating effects of untreated PTSD include high rates of homelessness, violence, drug abuse, suicidality and a decreased quality of life. Little is known as to the breadth of NPs knowledge of PTSD sequelae, epidemiology and screening protocols. Increased perceived self-efficacy, tied to positive attitudes about PTSD knowledge and screening, result in overall higher rates …

Reassessing The Transcriptional Regulation Of Protective Cd4 T Cell Responses Against Influenza A Virus, Kunal Dhume

Electronic Theses and Dissertations, 2020-2023

An attractive strategy to improve efficacy of current Influenza A Virus (IAV) vaccines is to promote protective CD4 T cell responses. Antiviral CD4 T cell responses are predominantly classified as strong IFN-gamma producing T helper type 1 (Th1) cells. Yet, the role of IFN-gamma in protection against IAV is still unclear, suggesting absolute Th1 polarization may be expendable for effective IAV immunity. Here we test this hypothesis using models that restrict the deficiency of known transcriptional regulators of Th1 immunity in only CD4 T cells, avoiding indirect impact on other immune cell responses. We find the 'master regulator' of Th1 …

Housing Is Healthcare Measuring The Cross-Sector Impact Of A Major Housing Initiative In Portland, Oregon, Keri B Vartanian, Aisha K Gilmore, Kyle Jones

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No abstract provided.

Development And Evaluation Of Wound Healing Property Of In-Situ Hydrogel Formulation For Topical Delivery Of Short-Chain Hyaluronic Acid In Dental Use, Porniweat Rosaming

Chulalongkorn University Theses and Dissertations (Chula ETD)

Regeneration of damaged periodontal tissues becomes one of the biggest challenges that is hard to overcome, even without the presence of any pathogens. Tissue engineering was proposed as a possible technique for the lost tissues’ restoration. In this study, methacrylated hyaluronic acid (MeHA) hydrogel was developed with different concentrations of low molecular weight (short-chain) hyaluronic acid to achieve the hydrogels with suitable physical properties and biological properties for dental tissue regeneration. The results revealed that short-chain hyaluronic acid (sHA) could interfere with some behaviors of networking systems; however, overall properties (including gelation time, mechanical properties, swelling and degradation profiles, and …

Confidence, Interest And Intentions Of Final-Year Nursing Students Regarding Employment In General Practice, Kaara Ray B. Calma, Susan Mcinnes, Elizabeth Halcomb, Anna Williams, Marijka Batterham

Nursing Papers and Journal Articles

Background: Rising health care burden has increased demand for general practice nurses. Exploring final-year nursing students’ perceived levels of confidence, interest and intention to work in this area can inform preparation and recruitment of new graduates into this workforce.

Aim: To explore final-year nursing students’ confidence, interest and intention to work in general practice.

Methods: Final-year nursing students from five universities situated in New South Wales, Australia were surveyed between March and June 2019. The survey comprised investigator-developed questions and validated tools adapted for use in general practice.

Findings: Of the 355 included responses, 34.1% respondents had a clinical placement …

Effectiveness Of Online Communication Skills Training For Cancer And Palliative Care Health Professionals: A Systematic Review, Melissa N. Berg, Irene Ngune, Penelope Schofield, Lisa Grech, Ilona Juraskova, Michelle Strasser, Zoe Butt, Georgia K.B Halkett

Nursing Papers and Journal Articles

Objective: To determine the reported effect of online communication skills training (CST) on health professional (HP) communication skills and patient care outcomes in cancer and palliative care.

Methods: Primary research published in English between January 2003 and April 2019 was identified in bibliographic databases including Medline, Embase and Proquest (Prospero: CRD42018088681). An integrated mixed-method approach included studies describing a CST intervention and its effect, for cancer or palliative care HPs, delivered online or blended with an online component. Included studies' outcomes were categorised then findings were stratified by an evaluation framework and synthesised in an effect direction plot. Risk of …

Sustained Passing Performance Of Elite And Subelite Female Soccer Players Following A Female Match-Specific Exercise Protocol, Mikayla Lyons, Jenny Conlon, Amy Perejmibida, Paola Chivers, Christopher Joyce

Nursing Papers and Journal Articles

Purpose: This study examined the maintenance of passing performance following soccer-specific high-intensity intermittent exercise in elite (n = 9) and subelite (n = 11) Western Australian female soccer players (19.5 [2.5] y).

Methods: A total of 20 participants completed the Loughborough Soccer Passing Test (LSPT) prior to, during, and following 90 minutes of a modified, female-specific, individualized exercise protocol (Loughborough Intermittent Shuttle Test [LIST]) to simulate 2 halves of a soccer match. Performance in the LSPT was calculated by adding “raw time” to the accumulated “penalty time” for each test.

Results: Elite players recorded greater distances ( …

Clinicians' Delirium Treatment Practice, Practice Change, And Influences: A National Online Survey, Annmarie Hosie, Meera Agar, Gideon A. Caplan, Brian Draper, Stephen Hedger, Debra Rowett, Penny Tuffin, Seong Leang Cheah, Jane L. Phillips, Linda Brown, Manraaj Sidhu, David C. Currow

Nursing Papers and Journal Articles

Background: Recent studies cast doubt on the net effect of antipsychotics for delirium.

Aim: To investigate the influence of these studies and other factors on clinicians’ delirium treatment practice and practice change in palliative care and other specialties using the Theoretical Domains Framework.

Design: Australia-wide online survey of relevant clinicians.

Setting/participants: Registered nurses (72%), doctors (16%), nurse practitioners (6%) and pharmacists (5%) who cared for patients with delirium in diverse settings, recruited through health professionals’ organisations.

Results: Most of the sample (n=475): worked in geriatrics/aged (31%) or palliative care (30%); in hospitals (64%); and saw a new patient with delirium …

Community-Engaged Frap: A Mixed Methods Approach For Policy Change And Advocacy To Reduce Cancer Disparities In Hispanic Women, Autumn Kieber-Emmons Md, Mph, William L. Miller Md, Ma, Benjamin F. Crabtree

Department of Family Medicine

No abstract provided.

Supervisor Knowledge, Attitudes, And Interventions Methods Of Burnout Among Healthcare Providers, Wendy Deal

College of Graduate Studies: Theses & Dissertations

Context: Burnout is prevalent among healthcare providers. Research has identified evidence-based intervention and preventative methods for use against burnout. There is limited research surrounding perceptions of burnout among those that supervise healthcare providers. Purpose: To explore supervisors’ knowledge, attitudes, and interventions related to burnout of healthcare providers within a hospital or clinic setting. Design: Consensual qualitative research with emergent design Methods: Eight supervisors were interviewed using a semi-structured interview approach via virtual platform. Participants were asked questions that aimed to capture supervisor perceptions of burnout. Interview transcripts were coded based on categories and themes developed through a consensus process …

Protecting Life And Lung: Protected Areas Affect Fine Particulate Matter And Respiratory Hospitalizations In The Brazilian Amazon Biome., Derek Michael Sheehan

Graduate Student Theses, Dissertations, & Professional Papers

I assessed the impacts of upwind protected area coverage on local respiratory health within the Brazilian Amazon. A hypothesized mechanism is the legal prohibition of human ignited fires within protected areas, reducing particulate matter pollution, impacting respiratory health downwind. The connection between fires and respiratory diseases in the Amazon is well established (Smith et al. 2014; Rangel and Vogl 2019; Rocha and Sant’anna 2020). What is not well understood is the potential that government policies aimed at preventing ecosystem loss may also promote health and wellbeing, combining the UN sustainable development goals 3 and 15. Protected areas currently dominate government …

College Students' Mental Health And Drug Use Outcomes During The Stay-At-Home Order, Alexander T. Hain

Cal Poly Humboldt theses and projects

The COVID-19 pandemic has affected people of all ages causing increased drug usage and worsening mental health in addition to hospitalization and death. The current study investigated how the United States’ stay-at-home orders affected the mental health and drug use of young adult college students. This population is of particular interest because young adults are at most risk of drug use developing into addiction. Two hypotheses related to the self-medication hypothesis were investigated: (1) there will be a significant increase in drug use during the stay-at-home order when compared to drug use prior to the stay-at-home order and (2) feelings …

Effects Of A Dual-Task Paradigm On Tandem Gait Performance, Courtney Angela Perry

Cal Poly Humboldt theses and projects

In concussion management, a variety of cognitive and motor control tests, like the tandem gait, have been used for assessing the presence of a concussion injury at the sideline and through recovery. More recently, research suggests that introducing a secondary cognitive retention task during tandem gait (i.e., dual task) may provide a better assessment of concussion. The purpose of this study was to determine if a tandem gait test paired with a 6-digit retention is a valid and reliable tool. Participants completed three trials of both the single-task (ST) and dual-task (DT) tandem gait tests, and their average (MEAN) and …

Dexamethasone Serum Concentrations After Intravenous Administration In Horses During Race Training, S. Mcclure, C. K. Fenger, Bradley Brown, George Maylin, W. Duer, L. Dirikolu, Kimberly Brewer, Jacob Machin, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Dexamethasone (DXM) sodium phosphate is a widely used corticosteroid for inflammatory conditions in horses, regulated in racing jurisdictions in the USA by a 0.005 ng/ml serum/plasma threshold. This study seeks to describe serum concentrations of DXM at 48 and 72 h after intravenous administration of 20 mg DXM sodium phosphate over 1 to 5 days, and to identify a possible source of DXM overages. 74 horses (39 Thoroughbreds, 13 Standardbreds, 22 Quarter Horses) in active race training received 20 mg DXM sodium phosphate. Serum was collected before injection, at 48 and 72 h post last injection, and analysed by LC/MS-MS …

Grit And Mindset - Unrelated To Performance In A Motor Learning Task, Jacob Smith

Cal Poly Humboldt theses and projects

Objective Research has shown grit and mindset can be improved, but no known research has investigated the effect of learning a new skill on grit and growth mindset. While grit and mindset seem to predict success in education and success, it is also not clear if initial grit or mindset have an effect on motor skill acquisition. The purposes of this research are to investigate if grit and growth mindset are predictors of learning a new skill and if learning a new skill leads to improvement in grit and growth mindset. Methods 14 students enrolled in a motor learning course …

The Antitumor Effects Of Plasma-Activated Saline On Muscle-Invasive Bladder Cancer Cells In Vitro And In Vivo Demonstrate Its Feasibility As A Potential Therapeutic Approach, Hao Zhang, Jishen Zhang, Bo Guo, Hailan Chen, Dehui Xu, Michael G. Kong

Bioelectrics Publications

Muscle-invasive bladder cancer (MIBC) is a fast-growing and aggressive malignant tumor in urinary system. Since chemotherapy and immunotherapy are only useable with a few MIBC patients, the clinical treatment of MIBC still faces challenges. Here, we examined the feasibility of plasma-activated saline (PAS) as a fledgling therapeutic strategy for MIBC treatment. Our data showed that plasma irradiation could generate a variety of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in saline. In vivo tests revealed that pericarcinomatous tissue injection with PAS was effective at preventing subcutaneous bladder tumor growth, with no side effects to the visceral organs after …

Growth In A Biofilm Sensitizes Cutibacterium Acnes To Nanosecond Pulsed Electric Fields, Asia Poudel, Adenrele Oludiran, Esin B. Sözer, Maura Casciola, Erin B. Purcell, Claudia Muratori

Bioelectrics Publications

The Gram-positive anaerobic bacterium Cutibacterium acnes (C. acnes) is a commensal of the human skin, but also an opportunistic pathogen that contributes to the pathophysiology of the skin disease acne vulgaris. C. acnes can form biofilms; cells in biofilms are more resilient to antimicrobial stresses. Acne therapeutic options such as topical or systemic antimicrobial treatments often show incomplete responses. In this study we measured the efficacy of nanosecond pulsed electric fields (nsPEF), a new promising cell and tissue ablation technology, to inactivate C. acnes. Our results show that all tested nsPEF doses (250 to 2000 pulses, 280 ns pulses, …

Comprehensive Collagen Crosslinking Comparison Of Microfluidic Wet-Extruded Microfibers For Bioactive Surgical Suture Development, Amrita Dasgupta, Nardos Sori, Stella Petrova, Yas Maghdouri-White, Nick Thayer, Nathan Kemper, Seth Polk, Delaney Leathers, Kelly Coughenour, Jake Dascoli, Riya Palikonda, Connor Donahue, Anna A. Bulysheva, Michael P. Francis

Bioelectrics Publications

Collagen microfiber-based constructs have garnered considerable attention for ligament, tendon, and other soft tissue repairs, yet with limited clinical translation due to strength, biocompatibility, scalable manufacturing, and other challenges. Crosslinking collagen fibers improves mechanical properties; however, questions remain regarding optimal crosslinking chemistries, biocompatibility, biodegradation, long-term stability, and potential for biotextile assemble at scale, limiting their clinical usefulness. Here, we assessed over 50 different crosslinking chemistries on microfluidic wet-extruded collagen microfibers made with clinically relevant collagen to optimize collagen fibers as a biotextile yarn for suture or other medical device manufacture. The endogenous collagen crosslinker, glyoxal, provides extraordinary fiber ultimate tensile …

Cardioporation Enhances Myocardial Gene Expression In Rat Heart, Carly Boye, Sezgi Arpag, Nina Burcus, Cathryn Lundberg, Scott Declemente, Richard Heller, Michael Francis, Anna Bulysheva

Bioelectrics Publications

Damage from myocardial infarction (MI) and subsequent heart failure are serious public health concerns. Current clinical treatments and therapies to treat MI damage largely do not address the regeneration of cardiomyocytes. In a previous study, we established that it is possible to promote regeneration of cardiac muscle with vascular endothelial growth factor B gene delivery directly to the ischemic myocardium. In the current study we aim to optimize cardioporation parameters to increase expression efficiency by varying electrode configuration, applied voltage, pulse length, and plasmid vector size. By using a surface monopolar electrode, optimized pulsing conditions and reducing vector size, we …

The Hsp90 Inhibitor, Auy-922, Protects And Repairs Human Lung Microvascular Endothelial Cells From Hydrochloric Acid-Induced Endothelial Barrier Dysfunction, Ruben M.L. Colunga Biancatelli, Pavel Solopov, Betsy Gregory, John D. Catravas

Bioelectrics Publications

Exposure to hydrochloric acid (HCl) leads acutely to asthma-like symptoms, acute respiratory distress syndrome (ARDS), including compromised alveolo-capillary barrier, and respiratory failure. To better understand the direct effects of HCl on pulmonary endothelial function, we studied the characteristics of HCl-induced endothelial barrier dysfunction in primary cultures of human lung microvascular endothelial cells (HLMVEC), defined the involved molecular pathways, and tested the potentially beneficial effects of Heat Shock Protein 90 (HSP90) inhibitors. HCl impaired barrier function in a time- and concentration-dependent manner and was associated with activation of Protein Kinase B (AKT), Ras homolog family member A (RhoA) and myosin light …

Dietary Phytoestrogens Ameliorate Hydrochloric Acid-Induced Chronic Lung Injury And Pulmonary Fibrosis In Mice, Pavel Solopov, Ruben Manuel Luciano Colunga Biancatelli, Christiana Dimitropoulou, John D. Catravas

Bioelectrics Publications

We previously reported that female mice exhibit protection against chemically induced pulmonary fibrosis and suggested a potential role of estrogen. Phytoestrogens act, at least in part, via stimulation of estrogen receptors; furthermore, compared to residents of Western countries, residents of East Asian countries consume higher amounts of phytoestrogens and exhibit lower rates of pulmonary fibrosis. Therefore, we tested the hypothesis that dietary phytoestrogens ameliorate the severity of experimentally induced pulmonary fibrosis. Male mice placed on either regular soybean diet or phytoestrogen-free diet were instilled with 0.1 N HCl to provoke pulmonary fibrosis. Thirty days later, lung mechanics were measured as …

Moderate Heat-Assisted Gene Electrotransfer As A Potential Delivery Approach For Protein Replacement Therapy Through The Skin, Chelsea Edelblute, Cathryn Mangiamele, Richard Heller

Bioelectrics Publications

Gene-based approaches for protein replacement therapies have the potential to reduce the number of administrations. Our previous work demonstrated that expression could be enhanced and/or the applied voltage reduced by preheating the tissue prior to pulse administration. In the current study, we utilized our 16-pin multi-electrode array (MEA) and incorporated nine optical fibers, connected to an infrared laser, between each set of four electrodes to heat the tissue to 43 °C. For proof of principle, a guinea pig model was used to test delivery of reporter genes. We observed that when the skin was preheated, it was possible to achieve …

Moderate Heat-Assisted Gene Electrotransfer For Cutaneous Delivery Of A Dna Vaccine Against Hepatitis B Virus, Chelsea Edelblute, Cathryn Mangiamele, Richard Heller

Bioelectrics Publications

An estimated 350 million people are living with chronic Hepatitis B virus (HBV) worldwide. Preventative HBV vaccination in infants has reduced the disease burden; however, insufficient immunization programs and access obstacles leave vulnerable populations at risk for infection in endemic regions. Gene electrotransfer (GET) using a noninvasive multielectrode array (MEA) provides an alternative platform for DNA vaccination in the skin. DNA vaccines are nonlive and nonreplicating and temperature stable unlike their counterparts. In addition, their simple engineering allows them to be manufactured quickly at a low cost. In the current work, we present the combination of GET and moderate heating …

Stobe Photography Mapping Of Cell Membrane Potential With Nanosecond Resolution, Allen S. Kiester, Bennett L. Ibey, Zachary N. Coker, Andrei G. Pakhomov, Joel N. Bixler

Bioelectrics Publications

The ability to directly observe membrane potential charging dynamics across a full microscopic field of view is vital for understanding interactions between a biological system and a given electrical stimulus. Accurate empirical knowledge of cell membrane electrodynamics will enable validation of fundamental hypotheses posited by the single shell model, which includes the degree of voltage change across a membrane and cellular sensitivity to external electric field non-uniformity and directionality. To this end, we have developed a high-speed strobe microscopy system with a time resolution of ~ 6 ns that allows us to acquire time-sequential data for temporally repeatable events (non-injurious …

The Role Of Reactive Oxygen Species In The Immunity Induced By Nano-Pulse Stimulation, Siqi Guo, Niculina I. Burcus, Megan Scott, Yu Jing, Iurii Semenov

Bioelectrics Publications

Reactive oxygen species (ROS) are byproducts of tumor cells treated with Nano-Pulse Stimulation (NPS). Recently, ROS have been suggested as a contributing factor in immunogenic cell death and T cell-mediated immunity. This research further investigated the role of NPS induced ROS in antitumor immunity. ROS production in 4T1-luc breast cancer cells was characterized using three detection reagents, namely, Amplex Red, MitoSox Red, and Dihydroethidium. The efficiency of ROS quenching was evaluated in the presence or absence of ROS scavengers and/or antioxidants. The immunogenicity of NPS treated tumor cells was assessed by ex vivo dendritic cell activation, in vivo vaccination assay …

Effect Of He Plasma Jet Versus Surface Plasma On The Metabolites Of Acute Myeloid Leukemia Cells, Dehui Xu, Ning Ning, Yujing Xu, Wenjie Xia, Hai-Lan Chen, Michael G. Kong

Bioelectrics Publications

Cold atmospheric plasma, including plasma jet and surface plasma, can promote the apoptosis of cancer cells without causing significant damage to surrounding normal cells, which was hopeful to be applied to the clinical cancer therapy. However, experimental plasma devices used directly to clinical experiments has challenges in technology and methods, especially the difference in killing tumor cells efficiency of these two common plasma sources. Therefore, it is great necessity to explore the differences in treating tumors between different plasma sources. This paper achieved good killing efficiency by using two kinds of cold atmospheric plasma generating devices, namely plasma jet and …

Seasonal Impact In Admissions And Burn Profiles In A Desert Burn Unit, Syed F. Saquib, Joseph T. Carroll, Paul J. Chestovich

Department of Surgery Faculty Research

In much of the world, pattern of burn injuries can vary depending on the season. This study examines the seasonal impact of admissions, acuity, mortality, and resource utilization at an accredited burn center located in a desert climate. It is a retrospective analysis of patients admitted from March 1, 2014 to February 28, 2019 for acute burns. Patients were categorized into four groups according to each season based on their date of admission: Spring (March, April, May), Summer (June, July, August), Fall (September, October, November), and Winter (December, January, February).

A total of 1519 patients were included. 1016 were male …

Ziba, Ziba, Sherianne Schow, Brandi Kilmer, Heather Oman

TSOS Interview Gallery

Ziba, a promising medical student, fled Afghanistan in 2018 due to instability and for her safety. Life was difficult upon arrival in the United States. In Afghanistan Ziba was involved in national and international poetry, math and science competitions. Ziba went from having everything to starting completely over in a new country. Her anxiety and depression became extremely difficult to deal with She reminded herself who she was, what her passions were and in January 2019 started medical school while working part time as a cashier. Her hope for future arriving refugees is to have a mental health network established …