Medicine and Health Sciences Commons^™

Epigenetic Alterations Mediate Ipsc Normalization Of Dna-Repair Expression And Tnr Stability In Huntington's Disease, Peter A. Mollica, Martina Zamponi, John Reid, Deepak Sharma, Alyson E. White, Roy C. Ogle, Robert D. Bruno, Patrick C. Sachs

School of Medical Diagnostics & Translational Sciences Publications

Huntington's disease (HD) is a rare autosomal dominant neurodegenerative disorder caused by a cytosine-adenine-guanine (CAG) trinucleotide repeat (TNR) expansion within the HTT gene. The mechanisms underlying HD-associated cellular dysfunction in pluripotency and neurodevelopment are poorly understood. We had previously identified downregulation of selected DNA repair genes in HD fibroblasts relative to wild-type fibroblasts, as a result of promoter hypermethylation. Here, we tested the hypothesis that hypomethylation during cellular reprogramming to the induced pluripotent stem cell (iPSC) state leads to upregulation of DNA repair genes and stabilization of TNRs in HD cells. We sought to determine how the HD TNR region …

Type 2 Bovine Viral Diarrhea Virus NPro Suppresses Type I Interferon Pathway Signaling In Bovine Cells And Augments Bovine Respiratory Syncytial Virus Replication, Abdulrahman Abdulaziz A. Alkheraif

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Bovine viral diarrhea virus (BVDV) and bovine respiratory syncytial virus (BRSV) infections contribute to the bovine respiratory disease complex (BRDC), which is a multi-factorial disorder involving co-infections of viruses and bacteria including mycoplasma. BRDC causes great economic losses to the United States feedlot industry. BVDV infection induces immunosuppression in infected animals. BVDV N^pro binds and degrades the transcription factor interferon regulatory factor-3 (IRF-3) and effectively blocks type I interferon (type I IFN) expression in host cells. BRSV nonstructural proteins, NS1 and NS2, block activation of IRF-3. In calves, concurrent infection with BVDV and BRSV resulted in more severe clinical …

A Biomechanical Simulation Of Musculoskeletal Kinematics During Ambulation, Alex Thomas

Master's Theses (2009 -)

The purpose of this study was to validate a 3D musculoskeletal model in OpenSim and assess OpenSim’s ability to determine muscle-length variation during ambulation. An 18 camera motion capture system was used to analyze 20 healthy individuals between the ages of 18 and 35. Following data collection, the data was processed through OpenSim and Visual3D. The kinematic output processed through the OpenSim model was then compared to the kinematic output of the validated Visual3D model to validate the OpenSim model. Muscle fiber length data obtained from the same experimental data was compared to control data to assess OpenSim’s muscle analysis …

Orthodontic Treatment Planning Using Direct Visual Approximation Of Arch Length Discrepancy And Cephalometric Analyses, Kathleen Rouse Vaught

Master's Theses (2009 -)

Introduction: Space analysis and radiographic analyses are crucial elements in developing an orthodontic treatment plan. It is imperative that the orthodontist makes accurate measurements in order to come up with the most effective treatment options for each patient. Some practitioners refrain from direct measurements to determine arch length discrepancies and instead determine the amount of crowding by using direct visual approximation. In addition, more orthodontists do not routinely trace all cephalograms taken. If more and more orthodontists are using direct visual approximation to determine angular cephalometric measurements, it is important to assess the accuracy and reliability of these measurements. This …

Comparison Of Immediate Vs. Delayed Recall Of Orthodontic Information Following An Electronic Reminder, Michael Lenz

Master's Theses (2009 -)

Patients and parents in dentistry often do not remember important information disclosed during the informed consent process, so improvement in the delivery of information is needed. This study explored the effect of an emailed narrated instructional PowerPoint on the immediate vs. delayed recall and comprehension of informed consent information in orthodontics. Thirty-two subjects were recruited from a university and private practice orthodontic clinic. At the initial visit, subjects were alternately assigned to one of two groups and were presented with a mock orthodontic treatment plan and informed consent presentation for a pretend patient. Immediately following the presentation, the subjects’ verbal …

Orthodontic Open-Coil Spring Deactivation Forces Differ With Varying Activation Levels, Ryan Lubinsky

Master's Theses (2009 -)

Coil springs are used in orthodontics to deliver forces to move teeth. However, the optimal amount of activation to produce predictable, continuous deactivation forces, for the purpose of orthodontic tooth movement, is unclear. The purpose of this study was to evaluate the deactivation force characteristics of nickel-titanium open-coil springs after varying levels of activation. Methods: Four open-coil spring products were evaluated: Dentsply GAC 100 gram and 150 gram, Orthoclassic Orthodontics Medium 150 gram, and American Orthodontics nickel-titanium springs. Open-coil springs were compressed (activation) with a universal testing machine to 20, 40, 60, or 80% of original length (15 mm; n=15/product/activation …

Orthodontic Bond Strength Comparison Between Two Filled Resin Sealants, James Kolstad

Master's Theses (2009 -)

Introduction: Sealants are used in orthodontics to help prevent demineralization during treatment. The objective of this study was to determine if there is a difference in the shear bond strength (SBS) between two different resin sealants bonded to teeth. Materials and Methods: Extracted human premolars (n=20/group) were randomly divided and prepared by acid etching, followed by application of primer or sealant. Group 1, the control group, used Transbond XT Primer (3M Unitek). Groups 2 and 3 were prepared with the sealants L.E.D. Pro Seal (Reliance Orthodontic Products) and Opal Seal (Opal Orthodontics) respectively. Transbond XT Adhesive was applied to a …

Application Of Mechanical Vibration To Enhance The Stability After Orthodontic Treatment - A Micro - Ct Study, Nicolas Branshaw

Master's Theses (2009 -)

Introduction: Mechanical vibration (MV) possesses anti-resorptive properties that can possibly enhance the stability of the dentition during retention, lessening the potential for relapse of teeth to their initial positions. In this project, we established a mouse model and investigated the effects of MV on bone modeling during orthodontic retention. Materials and Methods: Thirty-two 14-week-old inbred strain C57BL/6 male mice were randomly assigned into four groups: 1) Control (N=6); 2) Mechanical Vibration (MV) (N =6); 3) Orthodontic Retention (OR) (N=9); and 4) Orthodontic Retention and Mechanical Vibration (ORMV) (N=11). All mice in OR and ORMV groups received approximately 10g of orthodontic …

Difference In Therapeutic Alliance: High-Conflict Co-Parents Vs Regular Couples, Andrea Mae Parady

Theses and Dissertations

The purpose of this research is to explore differences in the therapeutic alliance for High-Conflict Co-Parents (HCC) vs. Regular Couples. Therapeutic alliance refers to the relationship, consisting of a bond, and agreement on the tasks and goals of therapy, between at least two people in the therapeutic relationship. It was hypothesized that HCC clients would have lower therapeutic alliance scores compared to regular couple clients. The results supported this hypothesis. By identifying these differences, more research can be conducted to improve our understanding of how to strengthen the therapeutic alliance with HCC clients, leading to improved treatment of this population.

Association Of Catechol-Omethyltransferase (Comt Val158met) With Future Risk Of Cardiovascular Disease In Depressed Individuals - A Swedish Population-Based Cohort Study, Aysha Almas, Yvonne Forsell, Vincent Millischer, Jette Möller, Catharina Lavebrat

Section of Internal Medicine

Background: Catechol-O-methyltransferase (COMT Val¹⁵⁸Met) has been implicated in both depression and cardiovascular disease. The purpose of this study was to assess if COMT Val¹⁵⁸Met, which influences the COMT enzyme activity, has an effect on the risk of cardiovascular disease (CVD) in individuals with a history of depression and also to determine if the risk differs depending on gender.

Methods: Data from a longitudinal cohort study of mental health among Swedish adults was used. Depression was assessed twice 3 years apart for each participant, in 1998-2001 and 2001-2003. Saliva DNA was contributed by 4349 (41.7%) of the …

The Impact Of Non-Mandated Physical Training On Physiological Measures And Performance In Army Officers, Emily Garrett

Theses and Dissertations

The purpose of this study was to observe the physiological changes in body composition and fitness assessments in US Army Officers after 5 months of non-mandated exercise. Twenty two captains stationed at Fort Benning, Georgia volunteered to participate (age; 26.9£1.2 years, height; 1.76+0.6m, and weight; 83.1+11.1kg). Eleven participants remained in active units with mandated training (MT) while 11 participants were in positions where training was not mandated (NMT). Anthropometrics, body composition, cardiorespiratory fitness, anaerobic power, and muscular strength were measured in both groups before and after the intervention period (1812 weeks). At pre-intervention, MT and NMT were not different in …

Examining The Association Of Environmental Degradation And Poor Cardiovascular And/Or Respiratory Health Outcomes In A Disadvantaged Community, Khyati Niranjan Kantaria

Health Services Research Dissertations

Air pollution is associated with poor cardiovascular and/or respiratory health outcomes. The poor air quality in certain communities due to the emission of toxic air pollutants from industries and major roadways has been a growing concern. The main objective of this study was to examine whether residential proximity to environmental air pollution sources, individual-level risk factors (age, gender, and body mass index (BMI)) and number of years at same residence are associated with observed poor cardiovascular and/or respiratory health outcomes in the residents of Southeast community in Newport News, Virginia. Logistic regression was conducted to assess this association using the …

What Predicts Medication Therapy Management Completion Rates? The Role Of Community Pharmacy Staff Characteristics And Beliefs About Medication Therapy Management, Omolola A. Adeoye-Olatunde, Leslie M. Lake, Spencer G. Lourens, Rachel E. Morris, Margie E. Snyder

Department of Pharmacy Practice Faculty Publications

Objectives: To model the association between pharmacy technicians' attitudes and planned behaviors toward participating in medication therapy management (MTM) and MTM completion rates. Secondary objectives included 1) to compare pharmacy technician and pharmacist attitudes and planned behaviors toward participating in MTM and 2) to identify respondent and pharmacy demographic factors associated with MTM completion rates.

Design: A 27-item survey, adapted from a previously published survey tool based on the Theory of Planned Behavior (TPB), was used to collect respondent perceptions of MTM. Study procedures were approved by the institutional review board.

Setting and participants: Pharmacy technicians and pharmacists representing 116 …

Post-Traumatic Stress Disorder And Cardiovascular Diseases: A Cohort Study Of Men And Women Involved In Cleaning The Debris Of The World Trade Center Complex, Molly Remsch, Zoey Laskaris, Janine D. Flory, Consuelo Mora-Mclaughlin, Alfredo Morabia

Publications and Research

BACKGROUND: To determine whether post-traumatic stress disorder (PTSD) is a risk factor for myocardial infarction (MI) and stroke, beyond the expected effects from recognized cardiovascular risk factors and depression.

METHODS AND RESULTS: World Trade Center-Heart is an observational prospective cohort study of 6481 blue-collar first responders nested within the World Trade Center Health Program in New York City. Baseline measures in 2012 and 2013 included blood pressure, weight and height, and blood lipids. PTSD, depression, smoking, and dust exposure during the 2001 cleanup were self-reported. During the 4-year follow-up, outcomes were assessed through (1) interview-based incident, nonfatal MI, and stroke, …

Exploring Empathy In Medical Narratives, Adam Uhrig

All NMU Master's Theses

No abstract provided.

Pcornet Antibiotics And Childhood Growth Study: Process For Cohort Creation And Cohort Description, Jason P. Block, L. Charles Bailey, Matthew W. Gillman, Douglas Lunsford, Janne Boone-Heinonen, Lauren P. Cleveland, Jonathan Finkelstein, Casie E. Horgan, Melanie Jay, Juliane S. Reynolds, Jessica L. Sturtevant, Christopher B. Forrest

OHSU-PSU School of Public Health Faculty Publications and Presentations

OBJECTIVES: The National Patient-Centered Clinical Research Network (PCORnet) supports observational and clinical research using health care data. The PCORnet Antibiotics and Childhood Growth Study is one of PCORnet’s inaugural observational studies. We sought to describe the processes used to integrate and analyze data from children across 35 participating institutions, the cohort characteristics, and prevalence of antibiotic use.

METHODS:We included children in the cohort if they had at least one same-day height and weight measured in each of 3 age periods: 1) before 12 months, 2) 12 to 30 months, and 3) after 24 months. We distributed statistical queries that each …

Retrograde Axonal Transport Of Rabies Virus Is Unaffected By Interferon Treatment But Blocked By Emetine Locally In Axons., Margaret A. Macgibeny, Orkide O. Koyuncu, Christoph Wirblich, Matthias J. Schnell, Lynn W. Enquist

Department of Microbiology and Immunology Faculty Papers

Neuroinvasive viruses, such as alpha herpesviruses (αHV) and rabies virus (RABV), initially infect peripheral tissues, followed by invasion of the innervating axon termini. Virus particles must undergo long distance retrograde axonal transport to reach the neuron cell bodies in the peripheral or central nervous system (PNS/CNS). How virus particles hijack the axonal transport machinery and how PNS axons respond to and regulate infection are questions of significant interest. To track individual virus particles, we constructed a recombinant RABV expressing a P-mCherry fusion protein, derived from the virulent CVS-N2c strain. We studied retrograde RABV transport in the presence or absence of …

The Scoop, Vol. 6 Issue 3-4, Summer 2019, Health Sciences Library

Volume 6

Latest news and events from the Health Sciences Library in our monthly newsletter for Summer 2019.

An Exploratory Factor Analysis: Factors That Influence Employers Disposition To Hire And Retain Persons With Disabilities, Javier Nazario Rodriguez

Theses and Dissertations

Despite the laws that are in place to ensure equal employment opportunities for persons with disabilities, there continues to be a disparity in employment rates for persons with disabilities when compared to persons without disabilities. Understanding factors that influence employers disposition to hire and retain persons with disabilities is the first step towards developing a guide for professionals that provide direct job placement services to this population in developing an educational intervention plan that is conducive to employers being more receptive to hiring persons with disabilities.

Currently there are many studies that focus on employers’ attitudes towards hiring and retaining …

2018 Summer Newsletter, Morehead State University. Nursing Department.

Nursing Department Publications Archive

2018 Summer Newsletter of the Department of Nursing at Morehead State University.

Trametinib Prevents Mesothelial-Mesenchymal Transition And Ameliorates Abdominal Adhesion Formation, Edward J. Macarak, Christine Lotto, Deepika Koganti, Xiaoling Jin, Pete J. Wermuth, Anna-Karin Olsson, Matthew Montgomery, Joel Rosenbloom

Department of Dermatology and Cutaneous Biology Faculty Papers

Background

Intra-abdominal adhesions are a major cause of morbidity after abdominal or gynecologic surgery. However, knowledge about the pathogenic mechanism(s) is limited, and there are no effective treatments. Here, we investigated a mouse model of bowel adhesion formation and the effect(s) of an Federal Drug Administration-approved drug (trametinib) in preventing adhesion formation.

Materials and methods

C57BL/6 mice were used to develop a consistent model of intra-abdominal adhesion formation by gentle cecal abrasion with mortality rates ofEDA) which arises from alternative splicing of the fibronectin messenger RNA resulting in different protein isoforms. Trichrome staining assessed collagen deposition. Quantitative polymerase chain reaction …

Preparation And Characterization Of Functionalized Heparin-Loaded Poly-Ɛ-Caprolactone Fibrous Mats To Prevent Infection With Human Papillomaviruses, Daniela Gonzalez, Jorge Ragusa, Peter C. Angeletti, Gustavo F. Larsen

Department of Chemical and Biomolecular Engineering: Faculty Publications

In this study, heparin-loaded poly-ε-caprolactone (PCL) fibrous mats were prepared and characterized based on their physical, cytotoxic, thermal, and biological properties. The main objective of the work described here was to test the hypothesis that incorporation of heparin into a PCL carrier could serve as bio-compatible material capable of inhibiting Human Papillomavirus (HPV) infection. The idea of firmly anchoring heparin to capture soluble virus, vs. a slow heparin release to inhibit a virus in solution was tested. Thus, one material was produced via conventional heparin matrix encapsulation and electrohydrodynamic fiber processing in one step. A second type of material was …

Pollen Evidence Of Medicine From An Embalming Jar Associated With Vittoria Della Rovere, Florence, Italy, Karl Reinhard, Kelsey B. Lynch, Annie Larsen, Braymond Adams, Leon Higley, Marina Milanello Do Amaral, Julia Russ, Donatella Lippi, Johnica J. Morrow, Dario Piombino-Mascali

Karl Reinhard Publications

Various samples of human viscera fragments, sponges, and cloth were collected from embalming jars belonging to members of the Medici family of Florence. One jar was labeled with the name Vittoria della Rovere, who died in March of 1694. This jar contained viscera fragments that were identified as a section of collapsed intestine. The intestine of the Vittoria della Rovere sample contained a large concentration of pollen belonging to the Myrtaceae family. The Myrtaceae pollen was sometimes observed in clusters during analysis, which is indicative of purposeful ingestion of flowers, buds, or a substance derived from floral structures. Thus, the …

Disease Gene Classification With Metagraph Representations, Sezin Kircali Ata, Yuan Fang, Min Wu, Xiao-Li Li, Xiaokui Xiao

Research Collection School Of Computing and Information Systems

This chapter is based on exploiting the network-based representations of proteins, metagraphs, in protein-protein interaction network to identify candidate disease-causing proteins. Protein-protein interaction (PPI) networks are effective tools in studying the functional roles of proteins in the development of various diseases. However, they are insufficient without the support of additional biological knowledge for proteins such as their molecular functions and biological processes. To enhance PPI networks, we utilize biological properties of individual proteins as well. More specifically, we integrate keywords from UniProt database describing protein properties into the PPI network and construct a novel heterogeneous PPI-Keyword (PPIK) network consisting …

Detecting Personal Intake Of Medicine From Twitter, Debanjan Mahata, Jasper Friedrichs, Rajiv Ratn Shah, Jing Jiang

Research Collection School Of Computing and Information Systems

Mining social media messages such as tweets, blogs, and Facebook posts for health and drug related information has received significant interest in pharmacovigilance research. Social media sites (e.g., Twitter), have been used for monitoring drug abuse, adverse reactions to drug usage, and analyzing expression of sentiments related to drugs. Most of these studies are based on aggregated results from a large population rather than specific sets of individuals. In order to conduct studies at an individual level or specific groups of people, identifying posts mentioning intake of medicine by the user is necessary. Toward this objective we develop a classifier …

The Tragedy Of Child And Adolescent Suicide, Brad Hinman

Faculty Publications

"In 2015, the Centers for Disease Control and U.S. Department of Education reported that 20 to 28 percent of U.S. students in grades 6 to 12 have experienced bullying, 30 percent admitted to bullying others, and 70 percent witnessed bullying in some form. According to the report, children and young adults are affected by bullying and cyberbullying during their school-age years, and some even into adulthood.³ “School violence and bullying occurs throughout the world and affects a significant proportion of children and adolescents. It is estimated that 246 million children and adolescents experience school violence and bullying in …

Rehearsal Injuries In Soft-Soled Character Shoes: Injury Rate Of Female Collegiate Dancers Wearing Soft-Soled Character Shoes With 2.5 Or 3.0 Inch Heels In Musical Theatre Rehearsals, Evelyn Vaughan

Honors College Theses

High-heeled shoes (HHS) have been known to present biomechanical imbalances in lower-leg mechanics at the pedestrian level, but have not been thoroughly studied in the context of highly dynamic movement, such as dance. In order to analyze the effects of 2.5-inch and 3.0-inch soft-soled character shoes (CS) worn by female collegiate musical theatre dancers during rehearsals, selected subjects completed a written questionnaire, the star balance excursion test, and a heel raise test. It was predicted that both heel heights would promote irregularities in subjects’ sense of balance and lower leg stamina, while 3.0 inch CS would cause greater rates of …

The Fight For Birth, Jessica E. Brown

Research Briefs

No abstract provided.

Characterizing The Recognition Motif And Novel Substrates Of Carm1, Sitaram Gayatri

Dissertations and Theses (Open Access)

A limited pool of proteins attains vast functional repertoire due to posttranslational modifications (PTMs). Arginine methylation is a common posttranslational modification, which is catalyzed by a family of nine protein arginine methyltransferases or PRMTs. These enzymes deposit one or two methyl groups to the nitrogen atoms of arginine side-chains. Elucidating the substrate specificity of each PRMT will promote a better understanding of which signaling networks these enzymes contribute to. Although many PRMT substrates have been identified, and their methylation sites mapped, the optimal target motif for each of the nine PRMTs has not been systematically addressed. Here we describe the …

Alternative Financial Services As A Social Determinant Of Health In U.S. Metropolitan Statistical Areas, Courtney Hundley, Richard W. Wilson, John Chenault

Faculty and Staff Scholarship

Alternative financial services (AFS) such as payday lenders, pawn brokers, tax refund loans, and check cashers are more prevalent in minority and lower income neighborhoods. These are neighborhoods also found to have disparities in health, compared to more affluent neighborhoods and communities. The focus of this paper is to determine if any relationship exists between use of AFS and health disparities. Using data from a survey performed by the Federal Deposit Insurance Corporation (FDIC), we compared four banking variables to several measures of health for 85 metropolitan statistical areas (MSA) across the nation. The four banking variables all related to …