Medicine and Health Sciences Commons^™

Impact Of Increasing Glp-1 On Markers Of Inflammation, Glucose Control And Cardiovascular Risk Factors In Patients With Type 2 Diabetes, Kristina Sandstedt

Physician Assistant Scholarly Project Posters

There is a strong established relationship between diabetes and cardiovascular disease. Much of the latest research studies have identified a link between the inflammatory processes and the pathogenesis of both cardiovascular disease and diabetes. Specific inflammatory markers include: Interleukins 1,6,18; C-reactive protein, Fibrinogen, Tumor Necrosis Factor-α, PAI-I and cell adhesion molecules. As a result, there has been an emphasis on identifying therapeutic approaches that would improve both markers of inflammation and glucose control. The endocrine hormones known as incretins, glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropicpolypeptide (GIP), are produced in the gastrointestinal tract following ingestion of a meal. In individuals with …

Comparing Short Versus Long Term Antibiotics For Reducing Persistent Lyme Symptoms, Joe Webster

Physician Assistant Scholarly Project Posters

• Lyme disease is the most common tick borne illness in the United States with over 300,000 cases annually.

• Two main organizations called the Infectious Disease Society of America and the International Lyme and Associated Diseases Society differ on their opinions in the treatment duration of Lyme disease.

• A review of the literature explored studies that compared the effectiveness of short term versus long term antibiotics in the treatment of Lyme. Determining the most effective treatment method duration for reducing the long term risk of persistent symptoms of Lyme will be important for the improvement of the delivery …

Occupational Therapy Feeding And Eating Interventions For Autism Spectrum Disorders And Pervasive Developmental Disorders: A Systematic Review, Jordan T. Adolf, Hana M. Mattern

Occupational Therapy Capstones

Due to the limited evidence and lack of methodological rigor regarding feeding and issues in children with Autism Spectrum Disorders (ASD) and Pervasive Developmental Disorders (PDD), clinicians who treat children with these diagnoses rely on the limited amount of information and many are not aware of evidence-based interventions (Ahearn, Castine, Nault, & Green, 2001; Marshall, Hill, & Dodrill, 2013). The purpose of this scholarly project is to gather, critique, and determine efficacy of occupational therapy feeding and eating interventions for children with ASD and PDD.

We systematically reviewed literature for higher-level evidence, as defined by Level III evidence or above, …

Lumbar Radiculopathy Post Lumbar Discectomy: A Case Study, Amy Harmon

Physical Therapy Scholarly Projects

Background and Purpose: Low back pain (LBP) is one of the most common and costly musculoskeletal diagnosis in America. Approximately 50% of individuals with LBP have recurrent episodes by 1 year, 60% by 2 years, and 70% by 5 years. Micro-lumbar discectomies are often performed to relieve the radiculopathy and pain associated with nerve root entrapment. The purpose of this case study was to describe the clinical decision process implemented for the post-operative assessment and intervention of a patient with a L4-5, L5-S1 discectomy and to outline the associated outcomes.

Case Description: The patient, a 26 year-old male, …

Stepping On: Gait Velocity And Fall Risk Assessment, Brittney Herbst

Physical Therapy Scholarly Projects

Purpose: To identify fall risk and determine if the Stepping On program is effective at reducing the risk of falls in participants by increasing gait speed to greater than or equal to 1 m/s. Step and stride length were also compared to age related norms to determine if they correlated to fall risk. Gait speed of less than I m/s has been shown to increase the risk for falls in elderly.

Methods: Fourteen female participants over the age of 65 (mean age of 87) were recruited from a local Stepping On class conducted at an assisted living facility …

Physical Therapy Rehabilitation Following Arthroscopic Rotator Cuff Repair, Brittany E. Wirth

Physical Therapy Scholarly Projects

Background and Purpose: According to the American Academy of Orthopaedic Surgeons, previous studies have found the prevalence of rotator cuff tears may exceed 50% in individuals older than age 65. Since this age group is the fastest growing of the U.S. population, rotator cuff pathology is sure to become a more significant problem in the future. With this information, it is important to continue research on rotator cuff rehabilitation following arthroscopic surgery.

Case Description: This case report describes the physical therapy interventions and functional outcomes of a 58-year-old patient who underwent arthroscopic surgery to repair a partial tear …

Critical Illness Polyneuropathy, Complications, Erica E. Hjelmstad

Physical Therapy Scholarly Projects

Background and Purpose: Critical Illness Polyneuropathy/Myopathy results from the presence of sepsis, multi-organ or respiratory failure, septic inflammatory response syndrome, or difficulty weaning from the ventilator. Decreased amplitudes of compound muscle and sensory action potentials, and widespread denervation potentials in muscle are the primary cause for the resultant muscle weakness. The purpose of this paper is to report on the rehabilitation progress in a woman who developed CIP following surgical complications.

Case Description: A 63-year-old woman suffered from critical illness polyneuropathy following a complicated recovery following a sigmoidectomy secondary to diverticular disease. Multiple surgeries, inability to wean from …

Accessibility And Perceived Value Of Pre-Admission Clinical Contact Hours: A Physical Therapy Student Perspective, Katie Anderson, Megan Volden

Physical Therapy Scholarly Projects

Background and Purpose: The purposes of this study were to determine whether professional physical therapy students experience challenges obtaining pre-admission contact hours and if they found them to be beneficial. The results of the survey will be utilized to allow physical therapy programs to make informed decisions about admission requirements in regard to pre-admission contact hours.

Methods: This study was performed utilizing an electronic survey sent in an e-mail to program chairpersons or Directors of Clinical Education, asking them to distribute the survey to their respective current students. All accredited physical therapy programs in the United States were …

Shoulder Impingement, Jennifer Ramsay

Physical Therapy Scholarly Projects

Background. Women who undergo mastectomy are almost 6 times more likely to experience shoulder restriction and impairment than patients who undergo breast-conserving surgery. Many women in the U.S. will develop invasive breast cancer over the course of their lives. The purpose of this case study is to review the rehabilitation plan for a patient with shoulder dysfunction and a history of surgical intervention for breast cancer.

Case Description. The patient was referred to physical therapy with complaints of pain in the right shoulder and anterior chest which increased with overhead movement. The patient's medical history included a skin …

Emg Activity Of Accessory Muscles Of Breathing During Recovery Positions, Joel Kramer, Kelsey Meyer, Eric Nefstead, Daniel Vilaubi

Physical Therapy Scholarly Projects

Chronic obstructive pulmonary disease (COPD) is predicted to become the third leading cause of death worldwide between 2020 and 2030. Signs and symptoms of this disease can be debilitating, however techniques can be taught to decrease impairments. Suggested techniques for pulmonary recovery consist of supporting the upper extremities, supporting the head and leaning forward. The purpose of the current study was to examine the effects of recovery positions on the EMG activation of accessory muscles of respiration to decipher which recovery position is the most ideal for COPD patients. Fourteen healthy adults over the age of eighteen were recruited for …

Developmental Dysplasia Of The Hip: A Case Study, Joseph D. Taylor

Physical Therapy Scholarly Projects

PURPOSE: The primary purpose of this case study was to describe the implementation of a conservative treatment protocol for dysplasia of the hip in a young girl in an attempt to prevent surgical intervention. A secondary objective of reducing femoral acetabular impingement was subsequently undertaken in the course of the case study.

BACKGROUND: The patient was a 16 year old female with a diagnosis of bilateral developmental dysplasia of the hip (DOH), bilateral femoral acetabular impingement (FAI), anterior superior labral tear to left (L) hip, elevated antinuclear antibody (ANA) screening and a history of depression.

PLAN OF CARE …

Mckenzie Evaluation And Treatment For Lumbar Dysfunction And Anr, Michael Brooks

Physical Therapy Scholarly Projects

Background and Purpose: When it comes to low back pain, McKenzie theory is considered highly effective for evaluating and treating patients with low back symptoms. There are three main classifications within McKenzie theory; postural, dysfunction, and derangement syndromes. The main focus of this case will be on the dysfunction classification and its subcategory adherent nerve roots (ANR). Dysfunction syndrome is characterized by pain due to deformation of structures and tissues that limit ROM causing pain at end range. ANR's are caused by scar tissue build up encompassing nerve roots due to trauma or surgery that produces symptoms when nerve …

The Effectiveness Of The "Stepping On" Program For Reducing The Incidence Of Falls In The Elderly: Measured By Four Stage Balance Test, Julia Nelson

Physical Therapy Scholarly Projects

Introduction: The Stepping On program encompasses both a balance and strengthening exercise regimen that is targeted towards reducing falls in community-dwelling individuals over the age of 65, who have experienced a fall or are fearful of falling.

Purpose: The purpose of this study was to determine if the Four Stage Balance Test (FSBT) is an appropriate measure of fall risk in the Stepping On program and to determine if the Stepping On program is effective at reducing fall risk, measured by improved time performance.

Methods: Eight participants, all female, ages ranging from 80-94 years old (M=87.2) completed …

Electromyographic Analysis Of Hip Muscle Activity Comparing Maximal Voluntary Contraction To Manual Muscle Test Grades, Ty Bommersbach, Anthony Charbonneau,, Holly Koch, Braidy Solie

Physical Therapy Scholarly Projects

Introduction: The purpose of this study was to investigate the relationship between muscle activity during three manual muscle tests (MMT) and muscle activity during a maximal voluntary contraction (MVC) of the same muscle.

Subjects: Eleven female subjects voluntarily participated in this study. All participants were physical therapy students under the age of 30, nonpregnant, and without recent history of musculoskeletal injury.

Methods: Electromyographic (EMG) data was collected by placing surface electrodes over each participant's right tensor fasciae latae (TFL) and gluteus medius (GM) muscle. The EMG data from each muscle was transmitted by Noraxont TeleMy02400 G2 telemetry …

Exposing Second Year Medical Students To The Clinical Reasoning Process Prior To High Fidelity Simulation Learning, Phondie Simelane Simelane

Theses and Dissertations

Clinical reasoning is a highly complex process that is both difficult to impart and acquire (Bowen 2006, Custers 2005, Merriënboer 2010, Schmidt 2015). Second year medical learners appear to lack strategy to effectively step through the presented scenarios (Allen, personal communication, March 2, 2015). Though possessing a degree of background knowledge, immature clinical reasoning skills make data collection (focused history, focused review of systems and focused physical assessments) a challenge to efficiently navigate. As oppose to discriminating their line of questions, learners sweep through a wide range of information. Consequently, problem solving takes on a shot gun approach resulting in …

Identification Of Mild Freezing Shock Response Pathways In Barley Based On Transcriptome Profiling, Xiaolei Wang, Dezhi Wu, Qian Yang, Jianbin Zeng, Gulei Jin, Zhong-Hua Chen, Guoping Zhang, Fei Dai

Publications

Low temperature is a major abiotic stress affecting crop growth and productivity. A better understanding of low temperature tolerance mechanisms is imperative for developing the crop cultivars with improved tolerance. We herein performed an Illumina RNA-sequencing experiment using two barley genotypes differing in freezing tolerance (Nure, tolerant and Tremois, sensitive), to determine the transcriptome profiling and genotypic difference under mild freezing shock treatment after a very short acclimation for gene induction. A total of 6474 differentially expressed genes, almost evenly distributed on the seven chromosomes, were identified. The key DEGs could be classified into six signaling pathways, i.e., Ca(2+) signaling, …

The Relationship Of Chlamydia Antigen, Ad-Like Pathology, And Inflammation In The Brains Of Intranasally Infected Balb/C Mice, Brittany L. Kane

PCOM Biomedical Studies Student Scholarship

Alzheimer's' Disease (AD) is a progressive neurodegenerative disease that affects millions of elderly individuals in the western world. There are two forms of AD, sporadic late-onset AD and Familial AD. The neuropathy seen in both cases of AD is the same; neurofibrillary tangles (NFTs) that are composed of modified tau protein, and neuritic senile plaques (NSPs) composed of deposited beta amyloid peptide. The objective of this study is to continue to investigate the role of infection as a possible risk factor for sporadic AD. Previous mouse models using Chlamydia pneumoniae demonstrated that intranasal inoculation with this organism induced AD-like pathology …

Bioinnovation Enterprise: An Engine Driving Breakthrough Therapies., Scott A. Waldman, Andre Terzic

Department of Pharmacology and Experimental Therapeutics Faculty Papers

Biological advances have radically expanded our insights into the underpinnings of health and disease. New knowledge has formed the substrate for translation-expedited in turn by the biotechnology and pharmaceutical industry into novel therapeutic solutions impacting the management of patients and populations. Indeed, this Bioinnovation Enterprise has become the dominant growth sector in drug development and the engine driving the translation of breakthrough therapies worldwide. This annual Therapeutic Innovations issue highlights recent exceptional advances by the Bioinnovation Enterprise in translating molecular insights in pathobiology into transformative therapies.

Do Pressure Requirements Change Over Time With Auto Titrating Positive Airway Pressure Therapy For Obstructive Sleep Apnea?, Anupama Tiwari, Md, Karl Doghramji, Md, Ritu Grewal, Md

Department of Sleep Medicine Faculty Papers

Study Objective:

Prior studies have demonstrated reduced inflammation after chronic use of CPAP therapy. This may lead to reduced pressures over time as the upper airway becomes more compliant. We sought to determine if there was a reduction in 90th percentile pressure requirements in patients on Auto PAP for OSA.

Methods:

A retrospective chart review of 988 patients who underwent a sleep study at our sleep center was performed. Those with moderate to severe sleep apnea who were prescribed and compliant with Respironics Auto PAP were included, yielding a total of 46 patients for analysis. All statistical analysis analyses were …

A Hyperbranched Dopamine-Containing Peg-Based Polymer For The Inhibition Of Α-Synuclein Fibrillation, Leonid Breydo, Ben Newland, Hong Zhang, Anne Rosser, Carsten Werner, Vladimir N. Uversky, Wenxin Wang

Molecular Medicine Faculty Publications

Aggregation of α-synuclein is believed to play an important role in Parkinson's disease and in other neurodegenerative maladies. Small molecule inhibitors of this process are among the most promising drug candidates for neurodegenerative diseases. Dendrimers have also been studied for anti-fibrillation applications but they can be difficult and expensive to synthetize. Here we show that RAFT polymerization can be used to produce a hyperbranched polyethylene glycol structure via a one-pot reaction. This polymer included a dopamine moiety, a known inhibitor of α-synuclein fibril formation. Dopamine within the polymer structure was capable of aggregation inhibition, although not to the same degree …

Structure And Function Relationships Of Proteins Based On Polar Profile: A Review, Carlos Polanco, Thomas Buhse, Vladimir N. Uversky

Molecular Medicine Faculty Publications

Proteins in the post-genome era impose diverse research challenges, the main are the understanding of their structure-function mechanism, and the growing need for new pharmaceutical drugs, particularly antibiotics that help clinicians treat the ever- increasing number of Multidrug-Resistant Organisms (MDROs). Although, there is a wide range of mathematical-computational algorithms to satisfy the demand, among them the Quantitative Structure-Activity Relationship algorithms that have shown better performance using a characteristic training data of the property searched; their performance has stagnated regardless of the number of metrics they evaluate and their complexity. This article reviews the characteristics of these metrics, and the need …

Structure And Stability Of Recombinant Bovine Odorant-Binding Protein: Iii. Peculiarities Of The Wild Type Bobp Unfolding In Crowded Milieu, Olga V. Stepanenko, Denis O. Roginskii, Olesya V. Stepanenko, Irina M. Kuznetsova, Vladimir N. Uversky, Konstantin K. Turoverov

Molecular Medicine Faculty Publications

Contrary to the majority of the members of the lipocalin family, which are stable monomers with the specific OBP fold (a β-barrel consisting of a 8-stranded anti-parallel β-sheet followed by a short α-helical segment, a ninth β-strand, and a disordered C-terminal tail) and a conserved disulfide bond, bovine odorant-binding protein (bOBP) does not have such a disulfide bond and forms a domain-swapped dimer that involves crossing the α-helical region from each monomer over the β-barrel of the other monomer. Furthermore, although natural bOBP isolated from bovine tissues exists as a stable domain-swapped dimer, recombinant bOBP has decreased dimerization potential and …

Detection Of Productively Rearranged Tcr-Α V–J Sequences In Tcga Exome Files: Implications For Tumor Immunoscoring And Recovery Of Antitumor T-Cells, Thomas R. Gill, Mohammad D. Samy, Shanitra N. Butler, James A. Mauro, Wade J. Sexton, George Blanck

Molecular Medicine Faculty Publications

Tumor immunoscoring is rapidly becoming a universal parameter of prognosis, and T-cells isolated from tumor masses are used for ex vivo amplification and readministration to patients to facilitate an antitumor immune response. We recently exploited the cancer genome atlas (TCGA) RNASeq data to assess T-cell receptor (TcR) expression and, in particular, discovered strong correlations between major histocompatibility class II (MHCII) and TcR-α constant region expression levels. In this article, we describe the results of searching TCGA exome files for TcR-α V-regions, followed by searching the V-region datasets for TcR-α-J regions. Both primary and metastatic breast cancer sample files contained recombined …

A Widely Employed Germ Cell Marker Is An Ancient Disordered Protein With Reproductive Functions In Diverse Eukaryotes, Michelle A. Carmell, Gregoriy A. Dokshin, Helen Skaletsky, Yueh-Chiang Hu, Josien C. Van Wolfswinkel, Kyomi J. Igarashi, Daniel W. Bellott, Michael Nefedov, Peter W. Reddien, George C. Enders, Vladimir N. Uversky, Craig C. Mello, David C. Page

Molecular Medicine Faculty Publications

The advent of sexual reproduction and the evolution of a dedicated germline in multicellular organisms are critical landmarks in eukaryotic evolution. We report an ancient family of GCNA (germ cell nuclear antigen) proteins that arose in the earliest eukaryotes, and feature a rapidly evolving intrinsically disordered region (IDR). Phylogenetic analysis reveals that GCNA proteins emerged before the major eukaryotic lineages diverged; GCNA predates the origin of a dedicated germline by a billion years. Gcna gene expression is enriched in reproductive cells across eukarya – either just prior to or during meiosis in single-celled eukaryotes, and in stem cells and germ …

Smoking Correlates With Increased Cytoskeletal Protein-Related Coding Region Mutations In The Lung And Head And Neck Datasets Of The Cancer Genome Atlas, John M. Yavorski, George Blanck

Molecular Medicine Faculty Publications

Cancer from smoking tobacco is considered dependent on mutagens, but significant molecular aspects of smoking-specific, cancer development remain unknown. We defined sets of coding regions for oncoproteins, tumor suppressor proteins, and cytoskeletal-related proteins that were compared between nonsmokers and smokers, for mutation occurrences, in the lung adenocarcinoma (LUAD), head and neck squamous carcinoma (HNSC), bladder carcinoma (BLCA), and pancreatic adenocarcinoma ( PAAD) datasets from the cancer genome atlas (TCGA). We uncovered significant differences in overall mutation rates, and in mutation rates in cytoskeletal protein-related coding regions (CPCRs, including extracellular matrix protein coding regions), between nonsmokers and smokers in LUAD and …

Two Isoforms Of Yersinia Pestis Plasminogen Activator Pla: Intraspecies Distribution, Intrinsic Disorder Propensity, And Contribution To Virulence, Svetlana V. Dentovskaya, Mikhail E. Platonov, Tat’Yana E. Svetoch, Pavel Kh. Kopylov, Tat’Yana I. Kombarova, Sergey A. Ivanov, Rima Z. Shaikhutdinova, Lyubov’ V. Kolombet, Sadhana Chauhan, Vitaly G. Ablamunits, Vladimir L. Motin, Vladimir N. Uversky, Andrey P. Anisimov

Molecular Medicine Faculty Publications

It has been shown previously that several endemic Y. pestis isolates with limited virulence contained the I259 isoform of the outer membrane protease Pla, while the epidemic highly virulent strains possessed only the T259 Pla isoform. Our sequence analysis of the pla gene from 118 Y. pestis subsp. microtus strains revealed that the I259 isoform was present exclusively in the endemic strains providing a convictive evidence of more ancestral origin of this isoform. Analysis of the effects of the I259T polymorphism on the intrinsic disorder propensity of Pla revealed that the I259T mutation slightly increases the intrinsic disorder propensity of …

Structure And Stability Of Recombinant Bovine Odorant-Binding Protein: I. Design And Analysis Of Monomeric Mutants, Olga V. Stepanenko, Denis O. Roginskii, Olesya V. Stepanenko, Irina M. Kuznetsova, Vladimir N. Uversky, Konstantin K. Turoverov

Molecular Medicine Faculty Publications

Bovine odorant-binding protein (bOBP) differs from other lipocalins by lacking the conserved disulfide bond and for being able to form the domain-swapped dimers. To identify structural features responsible for the formation of the bOBP unique dimeric structure and to understand the role of the domain swapping on maintaining the native structure of the protein, structural properties of the recombinant wild type bOBP and its mutant that cannot dimerize via the domain swapping were analyzed. We also looked at the effect of the disulfide bond by designing a monomeric bOBPs with restored disulfide bond which is conserved in other lipocalins. Finally, …

Prothymosin-Α Variants Elicit Anti-Hiv-1 Response Via Tlr4 Dependent And Independent Pathways, G. Luca Gusella, Avelino Teixeira, Judith Aberg, Vladimir N. Uversky, Arevik Mosoian

Molecular Medicine Faculty Publications

Prothymosin α (ProTα) (isoform 2: iso2) is a widely distributed, small acidic protein with intracellular and extracellular-associated functions. Recently, we identified two new ProTα variants with potent anti-HIV activity from CD8+ T cells and cervicovaginal lavage. The first is a splice variant of the ProTα gene known as isoB and the second is the product of ProTα pseudogene 7 (p7). Similarly to iso2, the anti-HIV activity of both variants is mediated by type I IFN. Here we tested whether the immunomodulatory activity of isoB and p7 are also TLR4 dependent and determined their kinetic of release in response to HIV-1 …

Classifying Lipoproteins Based On Their Polar Profiles., Carlos Polanco, Jorge Alberto Castañón-González, Thomas Buhse, Vladimir N. Uversky, Rafael Zonana Amkie

Molecular Medicine Faculty Publications

No abstract provided.

Feasibility Of Integrating Tripterygium Wilfordii Into Modern Cancer Therapy For Increased Efficacy With Minimal Toxicity, Andy Vo

AUCTUS: The Journal of Undergraduate Research and Creative Scholarship

Cancer is the second leading cause of death in the U.S., and millions of novel cancer cases are being diagnosed each year. While chemotherapy and ionizing radiation are effective treatments against these malignant tumors, the adverse effects that accompany such treatments are devastating. In order to find alternative treatment methods with less side effects, we turn to Eastern herbal medicine. Recent scientific research has found that Tripterygium wilfordii, an herbal medicine traditionally used to treat inflammation in China, contains compounds (triptolide and celastrol) that prevent the growth of solid tumors, induce apoptosis, and prevent metastasis of developed tumors. Investigations on …