Medicine and Health Sciences Commons^™

Myricetin Inhibits Proliferation Of Cisplatin-Resistant Cancer Cells Through A P53-Dependent Apoptotic Pathway, Haizhi Huang, Allen Y. Chen, Xingqian Ye, Bingyun Li, Yon Rojanasakul, Gary O. Rankin, Yi Charlie Chen

Pharmaceutical Science and Research

Cisplatin is a commonly used drug for cancer treatment by crosslinking DNA, leading to apoptosis of cancer cells, resistance to cisplatin treatment often occurs, leading to relapse. Therefore, there is a need for the development of more effective treatment strategies that can overcome chemoresistance. Myricetin is a flavonoid from fruits and vegetables, showing anticancer activity in various cancer cells. In this study, we found myricetin exhibited greater cytotoxicity than cisplatin in two cisplatin-resistant ovarian cancer cell lines, OVCAR-3 and A2780/CP70, and it was less cytotoxic to the normal ovarian cell line IOSE-364. Myricetin selectively induced apoptosis in both cisplatin-resistant cancer …

Whole-Retina Reduced Electrophysiological Activity In Mice Bearing Retina-Specific Deletion Of Vesicular Acetylcholine Transporter, Jake Bedore, Amanda C. Martyn, Anson K. C. Li, Eric A. Dolinar, Ian S. Mcdonald, Stuart G. Coupland, Vania F. Prado, Marco A. Prado, Kathleen A. Hill

Anatomy and Cell Biology Publications

Background: Despite rigorous characterization of the role of acetylcholine in retinal development, long-term effects of its absence as a neurotransmitter are unknown. One of the unanswered questions is how acetylcholine contributes to the functional capacity of mature retinal circuits. The current study investigates the effects of disrupting cholinergic signalling in mice, through deletion of vesicular acetylcholine transporter (VAChT) in the developing retina, pigmented epithelium, optic nerve and optic stalk, on electrophysiology and structure of the mature retina. Methods & Results A combination of electroretinography, optical coherence tomography imaging and histological evaluation assessed retinal integrity in mice bearing retina-targeted (embryonic day …

Selective Generation Of Dopaminergic Precursors From Mouse Fibroblasts By Direct Lineage Conversion., Changhai Tian, Yuju Li, Yunlong Huang, Yongxiang Wang, Dapeng Chen, Jinxu Liu, Xiaobei Deng, Lijun Sun, Kristi Anderson, Xinrui Qi, Yulong Li, R. Lee Mosley, Xiangmei Chen, Jian Huang, Jialin C. Zheng

Journal Articles: Pharmacology & Experimental Neuroscience

Degeneration of midbrain dopaminergic (DA) neurons is a key pathological event of Parkinson's disease (PD). Limited adult dopaminergic neurogenesis has led to novel therapeutic strategies such as transplantation of dopaminergic precursors (DPs). However, this strategy is currently restrained by a lack of cell source, the tendency for the DPs to become a glial-restricted state, and the tumor formation after transplantation. Here, we demonstrate the direct conversion of mouse fibroblasts into induced DPs (iDPs) by ectopic expression of Brn2, Sox2 and Foxa2. Besides expression with neural progenitor markers and midbrain genes including Corin, Otx2 and Lmx1a, the iDPs were restricted to …

Native/Aboriginal Students Use Natural Health Products For Health Maintenance More So Than Other University Students, Fidji Gendron, Samiah Naji Alqahtani, Sarah Omar Alkholy, Dina Haque, Maria Pontes Ferreira

Nutrition and Food Science Faculty Research Publications

Background and aim: University student use of Natural Health Products (NHP) for health maintenance (HealthM) is assessed in Canada. We hypothesize greater use of NHP by Native/Aboriginal and female students. Demographic predictor variables and the top ten NHP used are determined.

Methods: A cross-sectional survey of 963 students (n=212 Native/Aboriginal; n=751 non-Native/Aboriginal) was conducted. χ2 and Fisher’s exact tests analyzed group differences. Multiple logistic regressions determined predictor variables of NHP use.

Results: Of 963 surveyed students, 268 (27.8%) used NHP for HealthM, while 695 students (72.2%) did not. More Native/Aboriginal students used commercial tobacco (47% vs. 13%, P<0.001) and NHP (67% vs. 45%, P<0.001) than non- Native/Aboriginal students. Gender was not associated with NHP use (P=0.527). Canadians used echinacea more than non-Canadians (Odds Ratio [OR]=4.96; 95% Confidence Interval [CI]: 1.2-21.0). Ginger (OR=0.39; 95% CI: 0.2-0.78) and garlic (OR=0.28; 95% CI: 0.13-0.6) were popular amongst non-Canadians. Native/Aboriginal students used homeopathics (OR=39.9; 95% CI: 8.6-185.4) and rat root (OR=56.73; 95% CI: 6.91-465.8). Chamomile was less used by males (OR=0.33; 95% CI: 0.13-0.83) and used more by upperclassmen (OR=2.6 95% CI: 1.3-5.3).

Conclusion: Homeopathics …

Chemically Related 4,5-Linked Aminoglycoside Antibiotics Drive Subunit Rotation In Opposite Directions, Michael R. Wasserman, Arto Pulk, Zhou Zhou, Roger B. Altman, John C. Zinder, Keith D. Green, Sylvie Garneau-Tsodikova, Jamie H. Doudna Cate, Scott C. Blanchard

Pharmaceutical Sciences Faculty Publications

Dynamic remodelling of intersubunit bridge B2, a conserved RNA domain of the bacterial ribosome connecting helices 44 (h44) and 69 (H69) of the small and large subunit, respectively, impacts translation by controlling intersubunit rotation. Here we show that aminoglycosides chemically related to neomycin-paromomycin, ribostamycin and neamine-each bind to sites within h44 and H69 to perturb bridge B2 and affect subunit rotation. Neomycin and paromomycin, which only differ by their ring-I 6'-polar group, drive subunit rotation in opposite directions. This suggests that their distinct actions hinge on the 6'-substituent and the drug's net positive charge. By solving the crystal structure of …

Assessment Of Chronic Dyspnea In Older Adults: Impact Of The Dyspnoea-12 Questionnnaire On Nurse Self-Efficacy, Illaria C. Moore

Nursing Capstones

No abstract provided.

Fruit And Vegetable Consumption, Fat Intake, And Physical Activity Participation In Relation To Socio-Demographic Factors Among Medically Underserved Adults, Amir Alakaam, Jennifer Lemacks

Faculty Publications

Fruit and vegetable intake as well as physical activity participation in Mississippi is consistently lower than recommendations. We conducted a cross-sectional study to examine fruit and vegetables consumption, fat intake, and moderate-intensity physical activity participation and how these variables relate to socio-demographic factors among medically underserved adults in south Mississippi. Fruit and vegetable consumption and fat intake along with physical activity participation and socio-demographic characteristics was collected from a sample of 161 (48 male and 113 female) adults in south Mississippi. A majority (81.9%) of participants reported consuming less than five servings of fruits and vegetables per day and 54% …

Pp2a Inhibitors Arrest G2/M Transition Through Jnk/Sp1-Dependent Down-Regulation Of Cdk1 And Autophagy-Dependent Up-Regulation Of P21, Fei-Ran Gong, Meng-Yao Wu, Meng Shen, Qiaoming Zhi, Ze-Kuan Xu, Rong Wang, Wen-Jie Wang, Yang Zong, Zeng-Liang Li, Yadi Wu, Binhua P. Zhou, Kai Chen, Min Tao, Wei Li

Markey Cancer Center Faculty Publications

Protein phosphatase 2A (PP2A) plays an important role in the control of the cell cycle. We previously reported that the PP2A inhibitors, cantharidin and okadaic acid (OA), efficiently repressed the growth of cancer cells. In the present study, we found that PP2A inhibitors arrested the cell cycle at the G2 phase through a mechanism that was dependent on the JNK pathway. Microarrays further showed that PP2A inhibitors induced expression changes in multiple genes that participate in cell cycle transition. To verify whether these expression changes were executed in a PP2A-dependent manner, we targeted the PP2A catalytic subunit (PP2Ac) using siRNA …

Asset Transfer Of Subsidiary Of Pt Askes (Persero) And Its Implications For The Utility Of Participant Health Security, Adhi Kristian

Journal of Indonesian Health Policy and Administration

This research aims to learn about PT AJII transformation process as well as the implications of the transfer concerning service delivery to the National Health Insurance beneficiary. This is a normative empirical research, analyzing provision of the prevailing laws, legal principles and of the practice by carrying out library and field research. Informants of the research were the Primary Commissioner of PT AJII, who acted as internal supervisor of the transfer, member of Social Security Agency Bodies, (BPJS) and experts of social security who provided information and solution about the problems researched. The data obtained were analyzed by using qualitative …

Analysis On Policy Implementation Of Community Health Center As Report Obligation Recipient Institution For Narcotic Addicts In Jakarta Province In 2014, Sri Puji Wahyuni, Adang Bachtiar

Journal of Indonesian Health Policy and Administration

This paper analyzed the Policy Implementation of Community Health Center (CHC) as Report Obligation Recipient Institution for Narcotic Addicts in Jakarta Province in 2014. The design of the study employed a mixed methods approach of Sequential Explanatory. The implementation analysis employed the model developed by Donald Van Meter and Carl Van Horn. Within the model, policy implementation was associated with performance. The relationship was influenced by six variables: standards and targets, resources, communication among implementing agencies, implementing agency characteristics, attitudes of the implementers, as well as social, economic, and political factors. We found that variations of those six variables influenced …

2014- 2015 Clinical Guidelines And Recommendations, Beth Careyva Md

Department of Family Medicine

No abstract provided.

Reversal Of Aging-Related Neuronal Ca2+ Dysregulation And Cognitive Impairment By Delivery Of A Transgene Encoding Fk506-Binding Protein 12.6/1b To The Hippocampus, John C. Gant, Kuey-Chu Chen, Inga Kadish, Eric M. Blalock, Olivier Thibault, Nada M. Porter, Philip W. Landfield

Pharmacology and Nutritional Sciences Faculty Publications

Brain Ca(2+) regulatory processes are altered during aging, disrupting neuronal, and cognitive functions. In hippocampal pyramidal neurons, the Ca(2+)-dependent slow afterhyperpolarization (sAHP) exhibits an increase with aging, which correlates with memory impairment. The increased sAHP results from elevated L-type Ca(2+) channel activity and ryanodine receptor (RyR)-mediated Ca(2+) release, but underlying molecular mechanisms are poorly understood. Previously, we found that expression of the gene encoding FK506-binding protein 12.6/1b (FKBP1b), a small immunophilin that stabilizes RyR-mediated Ca(2+) release in cardiomyocytes, declines in hippocampus of aged rats and Alzheimer's disease subjects. Additionally, knockdown/disruption of hippocampal FKBP1b in young rats augments neuronal Ca(2+) responses. …

Profile Of Veliparib And Its Potential In The Treatment Of Solid Tumors, Lars M. Wagner

Pediatrics Faculty Publications

Inhibition of poly(ADP-ribose) polymerase (PARP) is an attractive therapeutic strategy because of the importance of this pathway in restoring DNA damage. Small-molecule inhibitors of PARP appear most effective when used to treat tumors with underlying defects in DNA repair, or when combined with DNA-damaging agents. Veliparib is one of several recently developed oral inhibitors of PARP currently in clinical trials. This review summarizes the pharmacology, mechanisms of action, toxicity, and activity of veliparib seen in clinical trials to date. Also discussed are proposed mechanisms of resistance, potential biomarkers of activity, and issues regarding patient selection and combination therapies that may …

Behavioral Health Integration In Pediatric Primary Care, Angelica Ventresca Msw, Lsw, Lauren Dennelly Msw, Lcsw

Department of Community Health and Health Studies

No abstract provided.

Hybrid Nanocrystals For Treatment And Bioimaging Of Disease, Tonglei Li

Pharmaceutical Sciences Faculty Patents

Hybrid nanocrystals able to reach specific targets in the body for treatment and biological imaging are provided, as well as methods of making and administering same for treatment of disease conditions and for bioimaging and radiotherapy. The hybrid nanocrystals and methods can be used alone or in combination with other treatment and imaging modalities.

Patent No. Us 9,090,589 B2: Specific Nnos Inhibitors For The Therapy And Prevention Of Human Melanoma, Richard B. Silverman, Frank L. Meyskens Jr., Sun Yang, Haitao Ji, Fengtian Xue, Thomas L. Poulos

Pharmacy Faculty Articles and Research

Methods for melanoma treatment and prevention with selective nitric oxide synthase inhibitor compounds and related pharmaceutical compositions, alone or in conjunction with one or more other melanoma therapies.

The Physics Of Human Performance: An Ideal Lab, William B. Laing Iii, Harold Mayer

Faculty Works

Physics lab goes to the gymnasium, where students calculate the mechanical power required to walk on an inclined treadmill in watts and convert to units power used to measure human performance: VO2, and METs. Students learn how to use two linear regression models: the ACSM "walking equation" to estimate the actual power expenditure of walking and the Rockport 1 mile test to estimate their own VO2max. Students use models to prescribe exercise parameters for themselves and for two cases. The IDEAL lab collaboration is developing labs that are open, applied to life, and rigorously quantitative.

Quantifying Measurement Error In Digital Instruments, William B. Laing Iii, Sean Bryant

Faculty Works

A first lab experiment clearly illustrates that a glucose meter is actually an excellent source of both random and systematic error, much to the surprise to students and physicians alike. A histogram is constructed and the utility of the standard deviation and standard error to quantify the uncertainty in each measurement and in the mean value, respectively, is demonstrated. From the first lab on, students are challenged to express and interpret confidence intervals in order to form quantitative conclusions. Assessments reveal that many students find this to be surprisingly challenging.

Repurposing Celecoxib As A Topical Antimicrobial Agent, Shankar Thangamani, Waleed Younis, Mohamed N. Seleem

Department of Comparative Pathobiology Faculty Publications

There is an urgent need for new antibiotics and alternative strategies to combat multidrug-resistant bacterial pathogens, which are a growing clinical issue. Repurposing existing approved drugs with known pharmacology and toxicology is an alternative strategy to accelerate antimicrobial research and development. In this study, we show that celecoxib, a marketed inhibitor of cyclooxygenase-2, exhibits broad-spectrum antimicrobial activity against Gram-positive pathogens from a variety of genera, including Staphylococcus, Streptococcus, Listeria, Bacillus, and Mycobacterium, but not against Gram-negative pathogens. However, celecoxib is active against all of the Gram-negative bacteria tested, including strains of, Acinetobacter, and Pseudomonas, when …

Numerical Chromosomal Instability Mediates Susceptibility To Radiation Treatment, Samuel F. Bakhoum, Lilian Kabeche, Matthew D. Wood, Christopher D. Laucius

Dartmouth Scholarship

The exquisite sensitivity of mitotic cancer cells to ionizing radiation (IR) underlies an important rationale for the widely used fractionated radiation therapy. However, the mechanism for this cell cycle-dependent vulnerability is unknown. Here we show that treatment with IR leads to mitotic chromosome segregation errors in vivo and long-lasting aneuploidy in tumour-derived cell lines. These mitotic errors generate an abundance of micronuclei that predispose chromosomes to subsequent catastrophic pulverization thereby independently amplifying radiation-induced genome damage. Experimentally suppressing whole-chromosome missegregation reduces downstream chromosomal defects and significantly increases the viability of irradiated mitotic cells. Further, orthotopically transplanted human glioblastoma tumours in which …

Paramedicine And Social Work: Case Studies In Authentic Student Recruitment, Nathalie Collins, Lynelle Watts, Renee Strauss, David Hodgson

eCULTURE

Selling the course experience to future students has been corporatised across the Higher Education sector. At many universities marketing and sales specialists, such as ECU’s Student Recruitment Team, rather than academic staff, field prospective student enquiries. Representing courses authentically is crucial to matching future students with an appropriate course experience and career, as well is managing future students’ expectations. A challenge for academics is communicating the course/career experience to university sales agents (recruiters). A challenge for recruiters is conveying an authentic course experience to future students when they have not taken a course themselves. This paper selects two ECU courses, …

Australian Midwifery Students' Views About Profession-Specific Peer Mentoring, Sara J. Bayes, Doreen Collyer, Kirsten Dobbs, Laura Walsh

eCULTURE

Midwifery is one of the most demanding professions there is, and midwifery students can find coping with the practice aspect of their course difficult. Mentoring is one way of supporting health clinicians’ emotional wellbeing; to date however, there is little research on mentoring for midwifery students. In this study, the aim of which was to discover midwifery students’ views of profession-related peer mentoring, qualitative and quantitative data were collected from 21 midwifery students at one Australian university. Analysis of the data revealed that most participants felt they would benefit from and would like to know more about mentoring. The qualitative …

Clinical Placement Before Or After Simulated Learning Environments? A Naturalistic Study Of Clinical Skills Acquisition Amongst Early-Stage Paramedicine Students, Brennen W. Mills Mr, Owen Carter A/Prof, Cobie Rudd Prof, Nathan Ross Mr, Louise Claxton Mrs

eCULTURE

Background

There is conflicting evidence surrounding the merit of clinical placements (CP) for early-stage health-profession students. Some contend early-stage CPs facilitate contextualisation of subsequently learned theory. Others argue attending CP before attaining skills competency is problematic and should only occur after training in simulated-learning environments (SLE). The evidentiary basis surrounding the extent to which either is true remains limited.

Methods

First-year paramedicine students (n=85) undertook three days of CP and SLEs as part of course requirements. Students undertook CP either before or after participation in SLEs creating two groups (Clin→Sim/Sim→Clin). Clinical skills acquisition was measured via objectively-structured clinical examinations (OSCE) …

Using Eye Tracking, Time-To-Action, Heart-Rate And Perceived Task Difficulty To Assess Level Of Distraction And Performance Of Entry-Level Paramedicine Students In Low- Versus High-Fidelity Simulation, Owen Carter, Brennen W. Mills, Cobie Rudd, Natalie Strobel, Louise Claxton, Nathan Ross

eCULTURE

Introduction

There is limited research investigating the usefulness of high-fidelity simulations (HFS) for early-stage students. Some argue the additional expense of HFS over low-fidelity simulation (LFS) is unnecessary as early-stage students are too easily distracted by non-relevant stimuli inhibiting student’s ability to focus on the core task. However, the extent to which this is true is yet to be empirically tested.

Methods

First-year paramedicine students were randomly assigned to complete either a LFS or HFS involving a collapsed patient with an obstructed airway. Level of distraction was measured via eye-tracking, arousal via heart-rate (HR), task difficulty via the NASA TLX, …

Veraflo Negative Pressure Wound Therapy With Household Bleach For Refractory Driveline Infection In A Patient With Left Ventricular Assist Device., Amr Idris, Amanda Hart, Sarah Branam, Maya Guglin

The VAD Journal

Driveline infections and sepsis are amongst the most common causes of morbidity and mortality in a patient with a left ventricular assist device. In this case, we present a patient with a multi drug resistant pseudomonas aeruginosa driveline exit site infection. Initially, it was treated with dual antibiotics, followed by surgical debridement utilizing Dakin’s solution irrigation and standard negative pressure wound therapy. Wound healing was minimal determined by poor granulation appearance, unchanged size from surgical debridement, purulent drainage, and the wound base remained colonized with multi drug resistant pseudomonas aeruginosa. A novel treatment of VeraFlo negative pressure wound therapy in …

Samhd1 Is A Single-Stranded Nucleic Acid Binding Protein With No Active Site-Associated Nuclease Activity., Kyle J. Seamon, Zhiqiang Sun, Luda S. Shlyakhtenko, Yuri L. Lyubchenko, James T. Stivers

Journal Articles: Pharmaceutical Sciences

The HIV-1 restriction factor SAMHD1 is a tetrameric enzyme activated by guanine nucleotides with dNTP triphosphate hydrolase activity (dNTPase). In addition to this established activity, there have been a series of conflicting reports as to whether the enzyme also possesses single-stranded DNA and/or RNA 3'-5' exonuclease activity. SAMHD1 was purified using three chromatography steps, over which the DNase activity was largely separated from the dNTPase activity, but the RNase activity persisted. Surprisingly, we found that catalytic and nucleotide activator site mutants of SAMHD1 with no dNTPase activity retained the exonuclease activities. Thus, the exonuclease activity cannot be associated with any …

What's Happening: July 27, 2015, Maine Medical Center

What's Happening

No abstract provided.

Mhealth Sms Text Messaging Interventions And To Promote Medication Adherence: An Integrative Review, Tracy Dekoekkoek, Barbara Given, Charles W. Given, Kimberly Ridenour, Monica Schueller, Sandra L. Spoelstra

Peer Reviewed Articles

Aims and objectives: This article is an integrative review of the evidence for mobile health Short Message Service text messages as an innovative and emerging intervention to promote medication adherence. Authors completed this review to draw conclusions and implications towards establishing a scientific foundation for use of text messages to promote medication adherence, thus informing clinical practice.

Background: The World Health Organization has identified medication adherence as a priority global problem. Text messages are emerging as an effective means of improving health behaviours and in some diseases to promote medication adherence. However, a gap in the literature indicates lack of …

Amikacin Concentrations Predictive Of Ototoxicity In Multidrug-Resistant Tuberculosis Patients, Chawangwa Modongo, Jotam G. Pasipanodya, Nicola M. Zetola, Scott M. Williams

Dartmouth Scholarship

Aminoglycosides, such as amikacin, are used to treat multidrug-resistant tuberculosis. However, ototoxicity is a common problem and is monitored using peak and trough amikacin concentrations based on World Health Organization recommendations. Our objective was to identify clinical factors predictive of ototoxicity using an agnostic machine learning method. We used classification and regression tree (CART) analyses to identify clinical factors, including amikacin concentration t

hresholds that pre- dicted audiometry-confirmed ototoxicity among 28 multidrug-resistant pulmonary tuberculosis patients in Botswana. Amika- cin concentrations were measured for all patients. The quantitative relationship between predictive factors and the probability of ototoxicity were then identified using …

Faculty Experience Of Flipping The Classroom: Lessons Learned, Melinda Hermanns, Jerri L. Post, Belinda Deal

Nursing Faculty Publications and Presentations

"Flipping the classroom" is gaining in popularity. The flipped classroom permits active learning and student engagement whereby the traditional class time is transformed into an active learning experience in which students can apply knowledge and interact with their peers and perform "hands-on" activities. This descriptive phenomenological study focused on the lived experiences of nursing faculty implementing a flipped classroom into the medical/surgical curriculum. The faculty's experience with implementing and adapting to non-traditional teaching learning methods of the "flipped"classroom in their medical/surgical classes is shared. The themes expressed by faculty are presented in the two overall categories of: Faculty concerns and …