Medicine and Health Sciences Commons^™

Modifying Antibody Dna For Site-Specific Binding, Madeline Gemoules

DePaul Discoveries

Humanized antibody plasmid DNA was modified to allow the distance between the Fc fragment and antigen binding sites of immunoglobulin G (IgG) antibodies to be studied. Specific variable heavy (VH) and variable light (VL) genes were inserted into heavy and light chain plasmids so that dye molecules can be easily attached to the expressed protein, and further inspection of antibody structure and function can be conducted via single molecule Förster resonance energy transfer (FRET). First, VH and VL genes were inserted into humanized antibody plasmids through the technique of ligation. The ligation product was then transformed into Escherichia coli cells, …

Single-Molecule Fluorescence Studies Of Glycosylated And Aglycosylated Antibodies, Irina Timoshevskaya

DePaul Discoveries

Antibodies are Y-shaped, flexible proteins whose structures can be studied using Förster Resonance Energy Transfer (FRET) at the single-molecule level. Dye molecules must be attached to these proteins so as to carry out FRET studies of antibodies. In order to label the binding sites of an antibody, dye molecules were attached to a small molecule, or hapten, which the antibody binds to. Evidence for this binding was provided by ultraviolet-visible (UV-Vis) spectroscopy. To label the stem region of a humanized immunoglobulin G (IgG) antibody, the DNA for this antibody was mutated to introduce a cysteine residue to which dyes can …

Soil And Leaf Lead Concentrations In The Lincoln Park Area, Agnes Kalat

DePaul Discoveries

Lead contamination in urban plants and soils has been a common occurrence as a result of human activities. The first objective of this experiment was to study the relationship between lead in soils and leaves: what is the source of lead in leaves found in urban trees? More specifically, does lead enter plants predominantly from the soil? It was hypothesized that if accumulation of lead occurred in the soil there would be a significant positive correlation between leaf and soil metal content. The second objective was to test if lead soil hotspots were associated with lead sources that have been …

Cortical Degeneration And Contusion Size Is Attenuated In Calpain-1 Knockout Mice Following A Controlled Cortical Impact (Cci), Stacey Seidl

DePaul Discoveries

Recent research has indicated that calcium-activated neutral proteases, calpains, are essential mediators of cell death in chronic and acute neurodegenerative disorders. Calpain activation has also been linked to Traumatic Brain Injury (TBI)-induced pathology. Specifically, two isoforms of calpain exist in the brain, calpain-2 and calpain-1, yet the specific roles of each enzyme following TBI are not well understood. Using calpain-1 knockout mice (KO), the current study examined the specific role of calpain-1 in neural degeneration following the controlled cortical impact (CCI) rodent model of TBI. Both Calpain-1 KO and wild type mice received a unilateral CCI over the forelimb sensorimotor …

Transplantation Of Adult Bone Marrow Stem Cells Enhances Behavioral Recovery Following A Traumatic Brain Injury (Tbi), Daniel J. Clark

DePaul Discoveries

A potential treatment for millions of new cases of traumatic brain injury (TBI) may be through the transplantation of genetically modified adult bone marrow stem cells (BMSC) in the form of neurospheres. This study examined behavioral recovery in the controlled cortical impact (CCI), a rodent model of TBI, after neurosphere transplantation. Rats received a unilateral CCI over the forelimb sensorimotor cortex. Seven days post-CCI, neurospheres or vehicle control were injected within the cortex or striatum. Forelimb deficits were assessed with two behavioral tests for two months. The test results indicated striatal neurosphere transplants significantly reduced deficits in both behavior tests …

Depression In Youth With Asthma: Asthma Severity, Exposure To Violence And Poverty, Draycen D. Decator

DePaul Discoveries

Youth with asthma have increasingly been the subjects of studies examining the effects of their chronic illness. The results of these studies have been mixed. A possible explanation for these inconsistent findings is that there are additional factors influencing these youth such as poverty and disease severity, and previous studies have not accounted for these differences. The current study was designed to help determine if there was a possible confounding variable (i.e., poverty) which strongly predicts depressive symptoms, and if asthma severity and exposure to violence moderate this relationship. Additionally, the current study looked for main effects between poverty, depressive …

Patterns And Predictors Of Self-Medication In Northern Uganda., Moses Ocan, Freddie Bwanga, Godfrey S. Bbosa, Danstan Bagenda, Paul Waako, Jasper Ogwal-Okeng, Celestino Obua

Journal Articles: Epidemiology

Self-medication with antimicrobial agents is a common form of self-care among patients globally with the prevalence and nature differing from country to country. Here we assessed the prevalence and predictors of antimicrobial self-medication in post-conflict northern Uganda. A cross-sectional study was carried out using structured interviews on 892 adult (≥18 years) participants. Information on drug name, prescriber, source, cost, quantity of drug obtained, and drug use was collected. Households were randomly selected using multistage cluster sampling method. One respondent who reported having an illness within three months in each household was recruited. In each household, information was obtained from only …

Substance Use Disorders And Stigma, Shirley S. Patrick

Regis University Student Publications (comprehensive collection)

Stigma is a problem for persons with substance abuse addictions and impacts access to health care services. The purpose of this project was to examine an educational program's effectiveness in reducing stigmatizing attitudes of health care professionals towards persons with substance abuse addictions. An educational program including a PowerPoint on substance abuse and stigma was presented to one group of nursing students and one group of Registered Nurses in a nurse residency program and pre-tests and post-tests completed. Results were calculated utilizing the paired samples ^-test. At the < 0 .05 level for a 2-tailed test only pairs seven, eight, and nine of the pre-test and post-test answers were statistically significant. Recommendations for future practice include educational programs targeting health care professionals. Stigma, social distance and discrimination are major obstacles to persons with addictions in obtaining mental health services.

Yoga Therapy For Irritable Bowl Syndrome: Integrative Medicine Grand Rounds, Daniel G. Kipnis, Msi

Jefferson Digital Commons News

Please join us for Integrative Medicine Grand Rounds on Tuesday, April 1, 2014 in 925 Chestnut Street, 2nd floor conference room from 8:00-9:00 am. Please note the venue for this exciting program. Senthamil R. Selvan, Ph.D.. is presenting and the title of his presentation is: “Yoga Therapy for Irritable Bowl Syndrome: Interim Results of a Randomized Control Study.”

Microfluidic-Enabled Liposomes Elucidate Size-Dependent Transdermal Transport, Renee R. Hood, Eric L. Kendall, Mariana Junqueira, Wyatt N. Vreeland, Zenaide M.N. Quezado, Julia C. Finkel, Don L. Devoe

Pediatrics Faculty Publications

Microfluidic synthesis of small and nearly-monodisperse liposomes is used to investigate the size-dependent passive transdermal transport of nanoscale lipid vesicles. While large liposomes with diameters above 105 nm are found to be excluded from deeper skin layers past the stratum corneum, the primary barrier to nanoparticle transport, liposomes with mean diameters between 31–41 nm exhibit significantly enhanced penetration. Furthermore, multicolor fluorescence imaging reveals that the smaller liposomes pass rapidly through the stratum corneum without vesicle rupture. These findings reveal that nanoscale liposomes with well-controlled size and minimal size variance are excellent vehicles for transdermal delivery of functional nanoparticle drugs.

Co2 Recycling Using Microalgae For The Production Of Fuels, Michael H. Wilson, John Groppo, Andrew Placido, S. Graham, S. A. Morton Iii, Eduardo Santillan-Jimenez, Aubrey Shea, Mark Crocker, Czarena Crofcheck, Rodney Andrews

Center for Applied Energy Research Faculty and Staff Publications

CO₂ capture and recycle using microalgae was demonstrated at a coal-fired power plant (Duke Energy’s East Bend Station, Kentucky). Using an in-house designed closed loop, vertical tube photobioreactor, Scenedesmus acutus was cultured using flue gas as the CO₂ source. Algae productivity of 39 g/(m² day) in June–July was achieved at significant scale (18,000 L), while average daily productivity slightly in excess of 10 g/(m² day) was demonstrated in the month of December. A protocol for low-cost algae harvesting and dewatering was developed, and the conversion of algal lipids—extracted from the harvested biomass—to diesel-range hydrocarbons via catalytic …

Today - March 21, 2014, Loma Linda University Health

TODAY

Inside this issue:

-- Loma Linda University Children's Hospital Foundation Gala adds first-ever benefit concert
-- Loma Linda University Health television show to air nationally on PBS's WORLD Channel beginning April 4
-- GRASP and GCAT awards highlight internally funded research at LLU
-- LLUH introduces new site for policy & procedure management
-- Riverside Convention Center to offer menu options designed by Loma Linda University Health
-- School of Pharmacy delivers flu shots to San Bernardino UPS facility
-- Human resources changes name to talent management services
-- School of Dentistry hosts successful 24th annual Clinic With A Heart …

Antiviral Resistance And Dynamic Treatment And Chemoprophylaxis Of Pandemic Influenza, Sandro Paz

USF Tampa Graduate Theses and Dissertations

Public health data show the tremendous economic and societal impact of pandemic influenza in the past. Currently, the welfare of society is threatened by the lack of planning to ensure an adequate response to a pandemic. This preparation is difficult because the characteristics of the virus that would cause the pandemic are unknown, but primarily because the response requires tools to support decision-making based on scientific methods. The response to the next pandemic influenza will likely include extensive use of antiviral drugs, which will create an unprecedented selective pressure for the emergence of antiviral resistant strains. Nevertheless, the literature has …

How To Get The Most From Microarray Data: Advice From Reverse Genomics, Ivan P. Gorlov, Ji-Yeon Yang, Jinyoung Byun, Christopher Logothetis, Olga Y. Gorlova, Kim-Anh Do, Christopher Amos

Dartmouth Scholarship

Whole-genome profiling of gene expression is a powerful tool for identifying cancer-associated genes. Genes differentially expressed between normal and tumorous tissues are usually considered to be cancer associated. We recently demonstrated that the analysis of interindividual variation in gene expression can be useful for identifying cancer associated genes. The goal of this study was to identify the best microarray data–derived predictor of known cancer associated genes. We found that the traditional approach of identifying cancer genes—identifying differentially expressed genes—is not very efficient. The analysis of interindividual variation of gene expression in tumor samples identifies cancer-associated genes more effectively. The results …

An Evaluation Of Group Contingency Interventions: The Role Of Teacher Preference, Christina Ennis

USF Tampa Graduate Theses and Dissertations

Disruptive behavior within classrooms is a major concern for teachers and parents. Positive Behavior Interventions and Supports (PBIS) provides a multi-tiered framework for schools to provide supports to students, which are matched to each student's needs. Whereas most students are successful with the school-wide supports provided to all students, approximately 20% of students are likely to require additional supports. Group contingencies have an established basis of support as effective Tier 2 interventions; however, these contingencies vary in a variety of dimensions that may influence their efficacy and acceptability. The purpose of this study was to evaluate the relative impact of …

Research Brief: "Housing Instability And Mental Distress Among Us Veterans", Institute For Veterans And Military Families At Syracuse University

Institute for Veterans and Military Families

This study highlights potential relationships between veteran mental health and veteran housing instability. This research shows that veterans reporting housing instability have an increased likelihood of symptoms of mental distress and mental illness, especially among female, younger, and unmarried veterans. Future researchers should conduct similar studies throughout the United States, as well as look at long-term data for the purpose of providing ways to improve quality of life for at-risk veterans.

Pathways Of Embodiment: Drug Use Among Adolescents In Popay[Aacute]N Colombia, Sarah Louise Fishleder

USF Tampa Graduate Theses and Dissertations

This research examines the interrelated biological and cultural factors that determine pathways by which recreational drug use is manifest to addiction in the lives of youth aged 12-18 in Popay[aacute]n, Colombia. It utilizes existing data from mixed methods research conducted between 2004-2005 examining epidemiological risk factors, drug use, perceptions about drugs, and a biological phenomenon of the brain known as incentive salience. Perceptions and experiences related to drugs were gathered using structured methods. MDS and hierarchical plots of drug perceptions are presented in order to demonstrate the power of culture and expectation on perception and choice. Structural equation modeling was …

Evaluation Of Hand Augered Well Technologies' Capacity To Improve Access To Water In Coastal Ng[Oumlaut]Be Communities In Panama, Sarah Hayman

USF Tampa Graduate Theses and Dissertations

Amid the global efforts surrounding United Nations' Millennium Development Goal Target 7c to improve access to safe and sustainable drinking water among populations who lack this resource, it has become essential to monitor and evaluate progress. Development initiatives working to achieve improved drinking water access often introduce appropriate technologies designed to be sustainably owned and operated by populations in rural areas suffering from water related hardships. It is valuable to thoroughly examine the degree to which these technologies satisfy intended objectives and affect user experienced water access. The accurate reflection of impact and progress can be complex, as the evaluation …

Access To Therapy For Children With Autism: A Population-Based Analysis, Teal W. Benevides

Theses and Dissertations

Children with autism spectrum disorder (ASD) represent a growing category of children who have special health care needs. Recent Centers for Disease Control and Prevention (CDC) estimates suggest that 1 in 88 children in the United States have an ASD (CDC, 2012). Due to difficulty with communication, social skills, and restricted and/or repetitive behaviors that comprise current diagnostic criteria (APA, 2013), children with ASD require significant medical, mental health, and therapeutic supports that contribute to greater heath care utilization costs than persons without ASD (e.g., Shimabukuro, Grosse, & Rice, 2008). Therapies such as occupational, physical, and speech therapy are among …

Multimodality Molecular Imaging Of [18f]-Fluorinated Carboplatin Derivative Encapsulated In [111in]-Labeled Liposome, Narottam Lamichhane

Theses and Dissertations

Platinum based chemotherapy is amongst the mainstream DNA-damaging agents used in clinical cancer therapy today. Agents such as cisplatin, carboplatin are clinically prescribed for the treatment of solid tumors either as single agents, in combination, or as part of multi-modality treatment strategy. Despite the potent anti-tumor activity of these drugs, overall effectiveness is still hampered by inadequate delivery and retention of drug in tumor and unwanted normal tissue toxicity, induced by non-selective accumulation of drug in normal cells and tissues. Utilizing molecular imaging and nanoparticle technologies, this thesis aims to contribute to better understanding of how to improve the profile …

Accuracy Of Educators In Identifying Middle School Students With Elevated Levels Of Anxiety Or Depression, Cheryl Gelley

USF Tampa Graduate Theses and Dissertations

Schools need accurate and efficient ways to identify youth with mental health problems, in part to provide services to such students whose mental health concerns pose barriers to learning. The present study involved an investigation of the accuracy of one method--relying on educators (teachers, a team of school-based mental health professionals, and a school nurse) to identify early adolescents who self-report elevated levels of anxiety or depression. With respect to teachers as screening agents, the possible variability in rates of accurate identification as a function of number of teachers involved in the process (i.e., pooling nominations from multiple subject area …

Isoform- And Species-Specific Control Of Inositol 1,4,5-Trisphosphate (Ip3) Receptors By Reactive Oxygen Species., Száva Bánsághi, Tünde Golenár, Muniswamy Madesh, György Csordás, Satish P. Ramachandrarao, Kumar Sharma, David I Yule, Suresh K Joseph, György Hajnóczky

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Reactive oxygen species (ROS) stimulate cytoplasmic [Ca(2+)] ([Ca(2+)]c) signaling, but the exact role of the IP3 receptors (IP3R) in this process remains unclear. IP3Rs serve as a potential target of ROS produced by both ER and mitochondrial enzymes, which might locally expose IP3Rs at the ER-mitochondrial associations. Also, IP3Rs contain multiple reactive thiols, common molecular targets of ROS. Therefore, we have examined the effect of superoxide anion (O2) on IP3R-mediated Ca(2+) signaling. In human HepG2, rat RBL-2H3, and chicken DT40 cells, we observed [Ca(2+)]c spikes and frequency-modulated oscillations evoked by a O2 donor, xanthine (X) + xanthine oxidase (XO), dose-dependently. …

Agenda: Fracking, Water Quality And Public Health: Examining Current Laws And Regulations, Network For Public Health Law, American Society Of Law, Medicine & Ethics, Public Health Law Research Program

Fracking, Water Quality and Public Health: Examining Current Laws and Regulations (March 20)

Improved technology developments in directional drilling and hydraulic fracturing, more commonly known as "fracking," have resulted in an oil and gas production boom nationwide. Fracking involves pumping pressurized water, sand, and chemicals down wells to crack bedrock, freeing petroleum and natural gas. Wastewater discharges, hydraulic fracturing fluid releases, and other accidental spills pose potential water quality risks, sparking concern for public health.

This webinar will examine the laws and regulations governing water quality issues related to fracking, recent state court decisions affecting regulations, and implications for public health.

Slides: Best Management Practices For Oil And Gas Development And Comparative Water Quality Database Of Regulations Relating To Shale Oil And Gas, Matt Samelson, University Of Colorado Boulder. Getches-Wilkinson Center For Natural Resources, Energy, And The Environment. Intermountain Oil And Gas Bmp Project

Fracking, Water Quality and Public Health: Examining Current Laws and Regulations (March 20)

Presenter: Matt Samelson, J.D., Attorney, Consultant for Intermountain Oil and Gas Best Management Practices (BMP) Project, Getches-Wilkinson Center for Natural Resources, Energy and the Environment, University of Colorado Law School

34 slides

Redox-Mediated And Ionizing-Radiation-Induced Inflammatory Mediators In Prostate Cancer Development And Treatment, Lu Miao, Aaron K. Holley, Yanming Zhao, William H. St. Clair, Daret K. St. Clair

Toxicology and Cancer Biology Faculty Publications

SIGNIFICANCE: Radiation therapy is widely used for treatment of prostate cancer. Radiation can directly damage biologically important molecules; however, most effects of radiation-mediated cell killing are derived from the generated free radicals that alter cellular redox status. Multiple proinflammatory mediators can also influence redox status in irradiated cells and the surrounding microenvironment, thereby affecting prostate cancer progression and radiotherapy efficiency.

RECENT ADVANCES: Ionizing radiation (IR)-generated oxidative stress can regulate and be regulated by the production of proinflammatory mediators. Depending on the type and stage of the prostate cancer cells, these proinflammatory mediators may lead to different biological consequences ranging from …

Hospitalization Of Children With Down Syndrome, Ariel Tenenbaum, Rana N. Hanna, Diana Averbuch, Isaiah D. Wexler, Maor Chavkin, Joav Merrick

Pediatrics Faculty Publications

INTRODUCTION: Children with Down syndrome present with multiple medical problems in a higher prevalence compared with the general population, which may lead to hospitalizations.

METHODS: Analysis of 560 hospitalizations of 162 children aged 0-16 years with Down syndrome at Hadassah Medical Center during the years 1988-2007 compared with data on children in the general population, hospitalized at the same period. Data was collected from patient files and statistical data from the Ministry of Health.

RESULTS: Respiratory infections were the leading cause for hospitalization of children with Down syndrome. The number of hospitalizations of children with Down syndrome compared to the …

Effects Of Graston Technique On Blood Flow In The Upper Extremity, Ashley Nicole Keck

Theses and Dissertations

Context: When musculature around the shoulder is tight, alterations in scapular positioning may result, thus compressing the blood vessels to the upper extremity and decreasing blood flow. Graston Technique (GT) mobilizes the soft tissues to break up adhesions in fascia and lengthens tight musculature. The effect of GT has on blood flow after treatment to the shoulder musculature is unknown. Objective: To determine if GT has an effect on blood flow and pectorals minor length after a single treatment session. Design: Controlled laboratory study with randomization. Setting: University athletic training laboratory. Patients or Other Participants: Sixty healthy subjects (27 male, …

Palatal Mucosa Derived Fibroblasts Present An Adaptive Behavior Regarding Cytokine Secretion When Grafted Onto The Gingival Margin, Fabíola Pontes Azevedo, Ana Carolina Morandini, Carla Renata Sipert, Thiago José Dionísio, Carlos Ferreira Santos, Carla Andreotti Damante, Maria Lúcia Rubo De Rezende, Adriana Campos Passanezi Sant'ana, Sebastião Luiz Aguiar Greghi

All Dugoni School of Dentistry Faculty Articles

BACKGROUND: Considering that grafted gingival tissue might have to be adapted to the receptor area and that fibroblasts have the ability to respond to bacterial stimuli through the release of various cytokines, this study investigated whether fibroblasts from the palatal mucosa behave differently when grafted onto the gingival margin regarding cytokine secretion.

METHODS: Biopsies from the palatal mucosa were collected at the time of free gingival graft surgery, and after four months re-collection was performed upon surgery for root coverage. Fibroblasts were isolated by the explant technique, cultured and stimulated with Porphyromonas gingivalis (Pg) and Escherichia coli (Ec) LPS for …

Microbiota, Oral Microbiome, And Pancreatic Cancer, Dominique S. Michaud, Jacques Izard

Department of Food Science and Technology: Faculty Publications

Only 30% of patients diagnosed with pancreatic cancer survive one year post-diagnosis. Progress in understanding the causes of pancreatic cancer has been made, including solidifying the associations with obesity and diabetes, and a proportion of cases should be preventable through lifestyle modifications. Unfortunately, identifying reliable biomarkers of early pancreatic cancer has been extremely challenging, and no effective screening modality is currently available for this devastating form of cancer. Recent data suggest the microbiota may play a role in the disease process, but many questions remain. Future studies focusing on the human microbiome, both etiologically and as a marker of disease …

Investigating A Role For The Ccaat/Enhancer-Binding Protein Δ In The Developing Zebrafish, Alisha Jennifer Beirl

Dissertations and Theses

The CCAAT/enhancer-binding protein delta (C/EBPδ) is a highly conserved transcription factor capable of regulating numerous cell fate processes, such as cell growth, differentiation, proliferation and apoptosis. C/EBPδ is inducible during cellular stress responses, including inflammation and responses to growth factor deprivation or thermal stress. C/EBPδ is stress-inducible in a diversity of fishes, including the zebrafish Danio rerio; however, little is known about its role in fish development. Here I show that overexpression of C/EBPδ leads to severe developmental defects, including reduced body length, edema, liver malformation and retinal abnormalities. The proportion of individuals that display developmental abnormalities is significantly …