Medicine and Health Sciences Commons^™

Challenges In Stem Cell Therapy: Bench-To-Bedside, Varunkumar G. Pandey, Michael Karsy

Quill & Scope

The article is a review of stem cell research and some of the challenges before the technology can become realizable patient therapy. We tried to make it a general review for individuals from various backgrounds.

At A Loss For Words: Language Choice In The Doctor’S Office, Evan Schloss

Quill & Scope

This piece is about language choice among bilingual populations (ie. code-switching and all the de-liberations between people when they decide which language to use), and its effects on the doctor-patient relationship.

The Modern Bloodletters, Charles Volk

Quill & Scope

When doctors order blood tests to be drawn, the order goes out to a team of people who have made bloodletting the sole focus of their job. Who are these people who have trained to complete this one task? The author shares his experiences drawing blood and what it is like to be ancillary staff at a large medical center.

Rapid Response Team Utilization Of Modified Early Warning Scores To Improve Patient Outcomes, Nikki L. Stoffel-Lowis

All Graduate Theses, Dissertations, and Other Capstone Projects

This retrospective, descriptive study was designed to (a) determine if the Modified Early Warning Score risk assessment tool identified moderate to high risk patients prior to the activation of the Rapid Response Team (b) determine how much time occurred from the onset of clinical deterioration until activation of the Rapid Response Team. A Modified Early Warning Score (MEWS) was applied to the documented vital signs in the medical records of a convenience sample of 108 adult patients between the ages of 19 and 99 years of age who had experienced an activation of the Rapid Response Team (RRT). A risk …

Needs Assessment For A Multidisciplinary Eating Disorder Outpatient Services, Mary Beth Patterson

All Graduate Theses, Dissertations, and Other Capstone Projects

Research has indicated there is limited specialist multidisciplinary care in non-metropolitan regions and rural communities for eating disorder treatment, leaving patients and professionals dissatisfied with quality of care. The purpose of this project was to assess the need for multidisciplinary eating disorder treatment service in South-Central Minnesota, and if so, to obtain information about the current patient population and treatment of such patients.

Utilizing Probabilistic Reinforcement To Enhance Participation In Parent Training, Errity Jones

USF Tampa Graduate Theses and Dissertations

Parental participation in parent training programs is necessary for success in behavioral parent training. Prior literature has demonstrated probabilistic reinforcement as an effective intervention for improving a wide variety of behaviors. In the present study, a probabilistic reinforcement program (i.e., lottery) was implemented in order to evaluate its efficacy as part of a behavioral parent training program. The behaviors targeted for increase included attendance, participation, homework completion, and performing role-plays or completing in-class assignments for two 10 week Tools for Positive Behavior Change courses. Participants earned lottery tickets for each of the dependent measures, and drawings took place at the …

The Meaning Of Adherence Among Veterans Receiving Pegylated Interferon And Ribavirin Antiviral Treatment For Chronic Hepatitis C, Frances Hinshaw Phillips

Nursing Dissertations - Archive

The purpose of this study was to describe the meaning of adherence and adherence experiences in hepatitis C (HCV) infected Veterans receiving pegylated interferon and ribavirin antiviral treatment. In addition, how these experiences influenced the Veteran‟s choice to remain adherent or non-adherent was vital to the concept of adherence. The theoretical framework used to guide this project was the Information, Motivation and Behavioral Skills Model of Adherence.A convenience sample of 21 HCV infected Veterans who were enrolled in a VA HCV treatment clinic were recruited. Those who were receiving antiviral treatment or who had recently completed antiviral treatment were eligible …

Mentoring Nurse Practitioners In A Hospital Setting, Rodica S. Pop

Nursing Dissertations - Archive

In 2009, the American Academy of Nurse Practitioners report identified more than 125, 000 Nurse Practitioners (NP) working in the United States. As the number of NPs increases and their role expands, questions about their work satisfaction and ability to learn and perform the role arise. Since NPs have to combine a nursing philosophy with a medical care model, it is often difficult to mediate the two approaches and fully understand the role. Mentoring has been successfully used to facilitate role transition and role understanding for nurses and physicians, but has been rarely studied in NPs. Although widely used in …

Strategies To Improve Respiratory Outcomes In Premature Infants With Respiratory Distress Syndrome, Patricia Thomas

Nursing Dissertations - Archive

Infants born prematurely with respiratory distress syndrome (RDS) are at high risk for complications from mechanical ventilation. Strategies are needed to minimize their days on the ventilator. The purpose of this study was to compare extubation success rates in infants treated with two different types of continuous positive airway pressure (CPAP). A retrospective cohort study design was used. Data were retrieved from electronic medical records for patients in a large, metropolitan, Level III neonatal intensive care unit. A sample of 194 premature infants with RDS were selected, 124 of whom were treated with nasal intermittent positive pressure ventilation (NIPPV) and …

Health Screenings Beyond A History Of Gestational Diabetes Mellitus: A Secondary Analysis Of The Behavioral Risk Factor Surveillance System, Johnnetta Phillips Kelly

Nursing Dissertations - Archive

Gestational diabetes mellitus (GDM) affects over 200,000 women in the United States each year placing them at a sevenfold risk of developing type 2 diabetes mellitus within a decade of delivery as compared to women without GDM. Diabetes has been projected to become more epidemic among women yet, a paucity of research has examined glucose screening trends beyond the postpartum period and longitudinal studies are few among those with a reproductive history of GDM. Suboptimal glucose screening and delayed diabetes detection can lead to an increased risk of cardiometabolic morbidity and mortality. Therefore, this study was designed to examine the …

Evaluation Of A Peer-Delivered, Strength-Based Family Education Program On Family Resilience Factors In Veterans Returning From Iraq And Afghanistan, Jane M. Harmon

Doctor of Nursing Practice (DNP) Scholarly Projects - Archive

Purpose: The purpose of this study was to evaluate the effects of participation in a peer-led, strength-based family education program focused on family resilience factors in returning OIF/OEF veterans and their families. 4 Data sources: De-identified data from Veterans returning from Iraq and Afghanistan and veteran family member participants in the 8-week Operation Resilient Families (ORP) program was analyzed. Data analysis included review of demographic information and statistical analysis of pre- and post self-assessment evaluation questionnaires. Results: Demographic data was provided on forty seven of fifty-three known participants - 89%. Data represented veterans and veteran family members, with nineteen (19) …

Ethnicity And Cultural Issues, John E. Zeber, Jodi M. Gonzalez, Richard Van Dorn, Alejandro Interian

Mental Health Law & Policy Faculty Publications

No abstract provided.

Depression Care Management: Can Employers Purchase Improved Outcomes?, Kathryn Rost, Donna Marshall, Benjamin Shearer, Allen J. Dietrich

Mental Health Law & Policy Faculty Publications

Fourteen vendors are currently selling depression care management products to US employers after randomized trials demonstrate improved work outcomes. The research team interviewed 10 (71.4%) of these vendors to compare their products to four key components of interventions demonstrated to improve work outcomes. Five of 10 depression products incorporate all four key components, three of which are sold by health maintenance organizations (HMOs); however, HMOs did not deliver these components at the recommended intensity and/or duration. Only one product delivered by a disease management company delivered all four components of care at the recommended intensity and duration. This “voltage drop,” …

Mechanism Of Resistance To Dietary Cholesterol, Lindsey R. Boone, Patricia A. Brooks, Melissa I. Niesen, Gene C. Ness

Molecular Medicine Faculty Publications

Background. Alterations in expression of hepatic genes that could contribute to resistance to dietary cholesterol were investigated in Sprague-Dawley rats, which are known to be resistant to the serum cholesterol raising action of dietary cholesterol. Methods. Microarray analysis was used to provide a comprehensive analysis of changes in hepatic gene expression in rats in response to dietary cholesterol. Changes were confirmed by RT-PCR analysis. Western blotting was employed to measure changes in hepatic cholesterol 7α hydroxylase protein. Results. Of the 28,000 genes examined using the Affymetrix rat microarray, relatively few were significantly altered. As expected, decreases …

Analyzing And Mapping Sweat Metabolomics By High-Resolution Nmr Spectroscopy, Viktor P. Kutyshenko, Maxim Molchanov, Peter Beskaravayny, Vladimir N. Uversky, Maria A. Timchenko

Molecular Medicine Faculty Publications

The content of human sweat is studied by high-resolution NMR, and the majority of organic components most often found in sweat of conditionally healthy people are identified. Original and simple tools are designed for sweat sampling from different areas of human body. The minimal surface area needed for sampling is in the range of 50–100 cm2. On all the surface parts of the human body examined in this work, the main constituents forming a sweat metabolic profile are lactate, glycerol, pyruvate, and serine. The only exception is the sole of the foot (planta pedis), where trace amounts of …

Predictive Power Estimation Algorithm (Ppea) - A New Algorithm To Reduce Overfitting For Genomic Biomarker Discovery, Jiangang Liu, Robert A. Jolly, Aaron T. Smith, George H. Searfoss, Keith M. Goldstein, Vladimir N. Uversky, Keith Dunker, Shuyu Li, Craig E. Thomas, Tao Wei

Molecular Medicine Faculty Publications

Toxicogenomics promises to aid in predicting adverse effects, understanding the mechanisms of drug action or toxicity, and uncovering unexpected or secondary pharmacology. However, modeling adverse effects using high dimensional and high noise genomic data is prone to over-fitting. Models constructed from such data sets often consist of a large number of genes with no obvious functional relevance to the biological effect the model intends to predict that can make it challenging to interpret the modeling results. To address these issues, we developed a novel algorithm, Predictive Power Estimation Algorithm (PPEA), which estimates the predictive power of each individual transcript through …

Effects Of Hmgn Variants On The Cellular Transcription Profile, Mark Rochman, Leila Taher, Toshihiro Kurahashi, Srujana Cherukuri, Vladimir N. Uversky, David Landsman, Ivan Ovcharenko, Michael Bustin

Molecular Medicine Faculty Publications

High mobility group N (HMGN) is a family of intrinsically disordered nuclear proteins that bind to nucleosomes, alters the structure of chromatin and affects transcription. A major unresolved question is the extent of functional specificity, or redundancy, between the various members of the HMGN protein family. Here, we analyze the transcriptional profile of cells in which the expression of various HMGN proteins has been either deleted or doubled. We find that both up- and downregulation of HMGN expression altered the cellular transcription profile. Most, but not all of the changes were variant specific, suggesting limited redundancy in transcriptional regulation. Analysis …

Critical Role Of Leucine-Valine Change In Distinct Low Ph Requirements For Membrane Fusion Between Two Related Retrovirus Envelopes, Marceline Côté, Yi-Min Zheng, Kun Li, Shi-Hua Xiang, Lorraine M. Albritton, Shan-Lu Liu

Nebraska Center for Virology: Faculty Publications

Many viruses use a pH-dependent pathway for fusion with host cell membrane, the mechanism of which is still poorly understood. Here we report that a subtle leucine (Leu)-valine (Val) change at position 501 in the envelope glycoproteins (Envs) of two related retroviruses, jaagsiekte sheep retro-virus (JSRV) and enzootic nasal tumor virus (ENTV), is responsible for their distinct low pH requirements for membrane fusion and infection. The Leu and Val residues are predicted to reside within the C-terminal heptad repeat (HR2) region of JSRV and ENTV Envs, particularly proximal to the hairpin turn of the putative six-helix bundle (6HB). Substitution of …

Hiv-1 Effects On Neuropsychological Performance In A Resource-Limited Country, Zambia, Gabriel Holguin, Mwanza Banda, Elizabeth J. Willen, Costantine Malama, Kaseya O. Chiyenu, Victor C. Mudenda, Charles Wood

Nebraska Center for Virology: Faculty Publications

Zambia has substantially been affected by the HIV/AIDS epidemic with prevalence rates at 14% in a population estimated at 12 million. Yet, the extent of HIV-associated neurocognitive disorders (HAND) in this population remains to be clearly understood. A series of culturally appropriate neuropsychological (NP) assessments [International HIV Dementia Scale (IHDS), Color Trails Test 1 and 2, Grooved pegboard Test, and Time Gait Test] were used to test the effects of HIV on NP performance of HIV seropositive and seronegative individuals. Twenty-two percent HIV positive individuals ARV naïve met the criteria for IHDS-defined NP impairment. Gender significantly influenced the performance on …

Characterization Of Species C Human Adenovirus Serotype 6 (Ad6), Eric A. Weaver, Reeti Khare, Mathew L. Hillestad, Donna Palmer, Philip Ng, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

Adenovirus serotype (Ad5) is the most studied Ad. Ad1, 2, and 6 are also members of species C Ad and are presumed to have biologies similar to Ad5. In this work, we have compared the ability of Ad1, 2, 5, and 6 to infect liver and muscle after intravenous and intramuscular injection. We found that Ad6 was surprisingly the most potent at liver gene delivery and that Ad1 and Ad2 were markedly weaker than Ad5 and 6. To understand these differences, we sequenced the Ad6 genome. This revealed that the Ad6 fiber protein is surprisingly three shaft repeats shorter than …

Generation Of A Kupffer Cell-Evading Adenovirus For Systemic And Liver-Directed Gene Transfer, Reeti Khare, Shannon M. May, Francesco Vetrini, Eric A. Weaver, Donna Palmer, Amanda Rosewell, Nathan Grove, Philip Ng, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

As much as 90% of an intravenously (i.v.) injected dose of adenovirus serotype 5 (Ad5) is absorbed and destroyed by liver Kupffer cells. Viruses that escape these cells can then transduce hepatocytes after binding factor X (FX). Given that interactions with FX and Kupffer cells are thought to occur on the Ad5 hexon protein, we replaced its exposed hypervariable regions (HVR) with those from Ad6. When tested in vivo in BALB/c mice and in hamsters, the Ad5/6 chimera mediated >10 times higher transduction in the liver. This effect was not due to changes in FX binding. Rather, Ad5/6 appeared to …

Molecular Bases Of Cutaneous And Uveal Melanomas, Sudeep Gaudi, Jane L. Messina

Pathology and Cell Biology Faculty Publications

Intensive research in recent years has begun to unlock the mysteries surrounding the molecular pathogenesis of melanoma, the deadliest of skin cancers. The high-penetrance, low-frequency susceptibility gene CDKN2A produces tumor suppressor proteins that function in concert with p53 and retinoblastoma protein to thwart melanomagenesis. Aberrant CDKN2A gene products have been implicated in a great many cases of familial cutaneous melanoma. Sporadic cases, on the other hand, often involve constitutive signal transduction along the mitogen-activated protein kinase (MAPK) pathway, with particular focus falling upon mutated RAS and RAF protooncogenes. The proliferative effects of the MAPK pathway may be complemented by the …

Primary Colorectal Adenocarcinoma Metastatic To The Breast: Case Report And Review Of Nineteen Cases, Rodney E. Shackelford, Pushpa Allam-Nandyala, Marilyn M. Bui, John V. Kiluk, Nicole Nicosia Esposito

Pathology and Cell Biology Faculty Publications

Metastases to the breast from extramammary primaries are uncommon and account for 0.5–6% of all breast malignancies (Georgiannos et al., 2001, and Vizcaíno et al., 2001). Malignant melanoma, lymphoma, and lung and gastric carcinomas are the most frequently encountered nonmammary metastases to the breast in adults (Georgiannos et al., 2001, and Chaignaud et al., 1994). Primary colorectal adenocarcinoma (CRC) metastatic to the breast is extremely rare, with the medical literature having only 19 recorded cases. Typically CRC metastatic to the breast is indicative of widely disseminated disease and a poor prognosis. Here we present a case of poorly differentiated colon …

Pathology Of Breast Carcinoma: Diagnostic, Prognostic, And Therapeutic Issues And Challenges, Rohit Bhargava, Sunati Sahoo, Nicole Nicosia Esposito, Beiyun Chen

Pathology and Cell Biology Faculty Publications

No abstract provided.

Novel Molecular Markers Of Malignancy In Histologically Normal And Benign Breast, Aejaz Nasir, Dung-Tsa Chen, Mike Gruidl, Evita B. Henderson-Jackson, Chinnambally Venkataramu, Susan M. Mccarthy, Heyoung L. Mcbrid, Eleanor Harris, Nazanin Khakpour, Timothy J. Yeatman

Pathology and Cell Biology Faculty Publications

To detect the molecular changes of malignancy in histologically normal breast (HNB) tissues, we recently developed a novel 117-gene-malignancy-signature. Here we report validation of our leading malignancy-risk-genes, topoisomerase-2-alpha (TOP2A), minichromosome-maintenance-protein-2 (MCM2) and “budding-uninhibited-by-benzimidazoles-1-homolog-beta” (BUB1B) at the protein level. Using our 117-gene malignancy-signature, we classified 18 fresh-frozen HNB tissues from 18 adult female breast cancer patients into HNB-tissues with low-grade (HNB-LGMA; ) and high-grade molecular abnormality (HNB-HGMA; ). Archival sections of additional HNB tissues from these patients, and invasive ductal carcinoma (IDC) tissues from six other patients were immunostained for these biomarkers. TOP2A/MCM2 expression was assessed as staining index (%) and …

Ovarian Epithelial-Stromal Interactions: Role Of Interleukins 1 And 6, Kamisha T. Woolery, Patricia A. Kruk

Pathology and Cell Biology Faculty Publications

Ovarian epithelial cancer is the most lethal gynecologic malignancy. The high mortality is attributed to the fact that most cases typically present in late stage when ovarian cancer (OC) has already spread beyond the ovary. Ovarian epithelial cancer cells are shed into intraperitoneal ascites and easily disseminate throughout the peritoneal cavity with preferential metastasis to the omentum, peritoneum, and local organs. Understanding how ovarian epithelial cells interact with and modulate their microenvironment can provide insight into the molecular mechanism(s) involved with malignant transformation and progression which may eventually identify novel diagnostic, prognostic, and therapeutic targets. The objective of this paper …

Toward Personalized Cell Therapies: Autologous Menstrual Blood Cells For Stroke, Maria Carolina O. Rodrigues, Loren E. Glover, Nathan Weinbren, Jessica A. Rizzi, Kazutaka Shinozuka, Naoki Tajiri, Yuji Kaneko, Paul R. Sanberg, Julie G. Allickson, Nicole Kuzmin-Nichols, Svitlana Garbuzova-Davis, Julio Cesar Voltarelli, Eduardo Cruz, Cesar V. Borlongan

Neurosurgery and Brain Repair Faculty Publications

Cell therapy has been established as an important field of research with considerable progress in the last years. At the same time, the progressive aging of the population has highlighted the importance of discovering therapeutic alternatives for diseases of high incidence and disability, such as stroke. Menstrual blood is a recently discovered source of stem cells with potential relevance for the treatment of stroke. Migration to the infarct site, modulation of the inflammatory reaction, secretion of neurotrophic factors, and possible differentiation warrant these cells as therapeutic tools. We here propose the use of autologous menstrual blood cells in the restorative …

Providing Mobile Librarian Service To A College Of Nursing To Increase Interactions And Transactions: One Wayne State University, Science And Technology Librarian's Experience, Nancy A. Wilmes

Library Scholarly Publications

A mobile librarian service to the Wayne State University College of Nursing was developed to provide in-person reference and research support. This service has been successfully used for eight semesters by College of Nursing faculty, graduate students, and some undergraduates for support of research and course work. This article will address the details and provide a description of the service.

Tanning Predicts Bone Mass But Not Structure In Adolescent Females Living In Hawaii, Daniel L. Osborne, Connie M. Weaver, Linda D. Mccabe, George P. Mccabe, Rachel Novotny, Carol Boushey, Dennis A. Savaiano

Department of Anthropology: Faculty Publications

Objectives: To evaluate the relationship between facultative skin pigmentation, which predicts circulating levels of plasma 25-hydroxymitamin D, and several measures of bone mass and structure in a cross sectional sample of adolescent females living in Hawaii.

Methods: Our sample was composed of adolescent females (n = 94) living in Hawaii where seasonal sun exposure is minimal, and who self-identified as either white (n = 16) or Asian (n = 78). Bone mineral content (BMC) of the total body, the lumbar spine and the hip, and cross sectional area (CSA) and section modulus (Z) at the …

Eastern Ichigan University Graduate Catalog, 2011-2012, Office Of The Registrar

Graduate Catalogs

No abstract provided.