Medicine and Health Sciences Commons^™

Rna-Dependent Selenocysteine Biosynthesis In Eukaryotes And Archaea, Sotiria Palioura

Yale Medicine Thesis Digital Library

Selenocysteine (Sec), the 21st genetically encoded amino acid, is the major metabolite of the micronutrient selenium. Sec is inserted into nascent proteins in response to a UGA codon. The substrate for ribosomal protein synthesis is selenocysteinyl-tRNASec. While the formation of Sec-tRNASec from seryl-tRNASec by a single bacterial enzyme selenocysteine synthase (SelA) has been well described, the mechanism of Sec-tRNASec formation in archaea and eukaryotes remained poorly understood. Herein, biochemical and genetic data provide evidence that, in contrast to bacteria, eukaryotes and archaea utilize a different route to Sec-tRNASec that requires the tRNASec-dependent conversion of O-phosphoserine (Sep) to Sec. In this …

Mature Dendritic Cells Use Endocytic Receptors To Capture And Present Antigens, Craig Daniel Platt

Yale Medicine Thesis Digital Library

In response to inflammatory stimuli, dendritic cells (DCs) trigger maturation, a terminal differentiation program required to initiate T lymphocyte responses. A hallmark of maturation is downregulation of endocytosis, widely assumed to restrict the ability of mature DCs to capture and present antigens encountered after the initial stimulus. We found that mature DCs continue to internalize antigens, especially by receptor-mediated endocytosis and phagocytosis. These antigens were transported to lysosomal compartments, loaded onto MHCII, and presented efficiently to T cells, both in vitro and in vivo. Antigens were also presented on MHCI with high efficiency. While mature DCs down-regulate macropinocytosis, they capture …

The Impact Of A Clinical Mentor Program On Patient Outcomes, Cynthia M. Steckel Phd

Dissertations

The increasing complexity of patient care requires an expert nurse to navigate the hospital stay, yet today's workforce brings a declining supply of seasoned nurses, creating a patient need/nurse expertise gap. The clinical mentor role was developed to bridge this gap and create a safety net, using expert nurses, relieved of a patient assignment, to provide oversight for quality patient care. Results reported elsewhere showed improvements in failure to rescue and pressure ulcers six months before and after implementation. The purpose of this study was to examine the long-term impact of this new care model over the subsequent three years …

Effects Of Reamer-Femoral Component Offset On Cement Mantle Penetration In Hip Resurfacing Arthroplasty, Mark Lloyd Paulick

Master's Theses

Hip resurfacing arthroplasty has changed the treatment of end stage arthritis without severe deformity for young, active adults. Presently, there are varying clinical approaches to implant design selection and cementation techniques. The purpose of this project is to determine what amount of reamer-femoral component offset allows for the best cement penetration into the femoral head.

Rapid prototyped femoral component models were produced with reamer femoral component offsets of 0.0 mm, 0.5 mm, and 1.0 mm. After implantation onto models of reamed femoral heads made from high-density open-cell reticulated carbon foam, cement penetration was assessed from cross-sections of the foam-implant unit. …

Endoscopic Repair Of High-Flow Cranial Base Defects Using A Bilayer Button., Adam J. Luginbuhl, Peter G. Campbell, James Evans, Marc Rosen

Department of Otolaryngology - Head and Neck Surgery Faculty Papers

OBJECTIVES/HYPOTHESIS: Repair of the skull base still begins with a direct repair of the dural defect. We present a new button closure for primary repair of the dura for high flow defects.

STUDY DESIGN: Retrospective review.

METHODS: We reviewed our 20 cases of primary button grafts and compared the results to the previous 20 high-flow open-cistern cerebrospinal fluid (CSF) cases. Subjects were excluded if they had no violation of the arachnoid space or potential for low-flow CSF leak. The button is constructed so that the inlay portion is at least 25% larger than the dural defect, and the onlay portion …

Perioperative Specialty Certification: The Cnor As Evidence For Magnet Excellence, Michelle Byrne, Kathryn Schroeter, Julie Mower

College of Nursing Faculty Research and Publications

No abstract provided.

A Preliminary Investigation Into The Effect Of Kinesio And Athletic Tape On Skin Blood Flow Changes, Ryan Klawon

Masters Theses

This research tested the effectiveness of a relatively new therapeutic product called Kinesio tape. One of Kinesio tape's therapeutic claims is increased blood flow to the taped area and thus expediting the body's healing process (Fu et aI., 2007). The purpose of this study was to compare the effects of Kinesio tape versus athletic tape on blood flow in the upper arm during rest and immediately following submaximal exercise. Certified athletic trainers (ATC) often use regular athletic tape to limit the range of motion and stabilize a joint as opposed to providing therapeutic benefits such as increasing blood flow.

Research …

Parental Report Versus Child Perception Of Familial Support: Which Is More Associated With Child Physical Activity And Television Use?, Daheia J. Barr-Anderson, Ramona Robinson-O'Brien, Jess Haines, Peter J. Hannan, Dianne R. Neumark-Sztainer

Faculty Publications

Background: Parent-report and child perception of familial support for weight-related behaviors may not be congruent. This research explores whether parent-report or child perception is more strongly associated with child-reported physical activity and television (TV) use. Methods: Elementary school children (n = 73) participating in Ready. Set. ACTION!, a theater-based obesity prevention pilot program in Saint Paul, MN, and their parents completed surveys assessing familial support for physical activity and limitations on TV use in fall 2006. Paired t tests examined congruency between parent-report and child perception. Linear regression models adjusted for sociodemographics explored the associations between familial …

The Pontine Respiratory Group, Particularly The Kölliker-Fuse Nucleus, Mediates Phases Of The Hypoxic Ventilatory Response In Unanesthetized Goats, Joshua M. Bonis, Suzanne E. Neumueller, K. L. Krause, T. Kiner, A. Smith, B. D. Marshall, B. Qian, Lawrence Pan, Hubert V. Forster

Physical Therapy Faculty Research and Publications

The objective of the present study was to test the hypothesis that, in the in vivo awake goat model, perturbation/lesion in the pontine respiratory group (PRG) would decrease the sensitivity to hypercapnia and hypoxia. The study reported herein was part of two larger studies in which cholinergic modulation in the PRG was attenuated by microdialysis of atropine and subsequently ibotenic acid injections neurotoxically lesioned the PRG. In 14 goats, cannula were bilaterally implanted into either the lateral (n = 4) or medial (n = 4) parabrachial nuclei or the Kölliker-Fuse nucleus (KFN, n = 6). Before and after …

Identification And Characterization Of Steroid Receptor Ligands, Megan Kathleen Dennis

Biomedical Sciences ETDs

This work focuses on steroid receptors, including the androgen receptor (AR), the estrogen receptors (ERs) ERα and ERβ, known as the classical ERs, and GPR30, a recently described G protein-coupled estrogen receptor. Each of these receptors plays a role in many normal tissues and disease pathologies and signaling through steroid receptors is required for growth of many cancers. Additionally, many tissues express more than one estrogen receptor, making delineation of the functions of individual receptors difficult. We have previously identified a GPR30-selective agonist, G-1, and this small molecule has been shown to mediate GPR30 function in a range of in …

Engineering Rna Phage Ms2 Virus-Like Particles For Peptide Display, Sheldon Keith Jordan

Biomedical Sciences ETDs

Phage display is a powerful and versatile technology that enables the selection of novel binding functions from large populations of randomly generated peptide sequences. Random sequences are genetically fused to a viral structural protein to produce complex peptide libraries. From a sufficiently complex library, phage bearing peptides with practically any desired binding activity can be physically isolated by affinity selection, and, since each particle carries in its genome the genetic information for its own replication, the selectants can be amplified by infection of bacteria. For certain applications however, existing phage display platforms have limitations. One such area is in the …

Perinatal Arsenic Exposure Inhibits Binding Ability Of Glucocorticoid Receptors To Nuclear Response Elements Altering Gene Expression And Affecting Learning Behavior, Ebany J. Martinez-Finley

Biomedical Sciences ETDs

Learning deficits in children following arsenic (As) exposure via drinking water have been epidemiologically described in the last decade. Arsenic is a persistent environmental toxin and exposure has been shown to perturb the hypothalamic-pituitary-adrenal (HPA) stress axis. The glucocorticoid receptors (GR) are an integral part of the HPA axis and are found throughout the central nervous system, particularly in the hippocampus, an area of the brain important in learning and memory. The mitogen-activated protein kinase (MAPK) pathway is involved in learning and two kinases of the MAPK pathway, Ras and Raf are transcriptionally regulated by GRs. In the studies detailed …

Commissioning An Anthropomorphic Spine And Lung Phantom For Remote Dose Verification Of Institutions Participating In Rtog 0631, Douglas F. Caruthers

Dissertations and Theses (Open Access)

The RPC developed a new phantom to ensure comparable and consistent radiation administration in spinal radiosurgery clinical trials. This study assessed the phantom’s dosimetric and anatomic utility. The ‘spine phantom’ is a water filled thorax with anatomy encountered in spinal radiosurgery: target volume, vertebral column, spinal canal, esophagus, heart, and lungs. The dose to the target volume was measured with axial and sagittal planes of radiochromic film and thermoluminescent dosimeters (TLD). The dose distributions were measured with the radiochromic film calibrated to the absolute dose measured by the TLD. Four irradiations were administered: a four angle box plan, a seven …

Osteopontin And Cadherin 11 Are Novel Mediators And Drug Targets For Chronic Lung Diseases, Daniel J. Schneider

Dissertations and Theses (Open Access)

Chronic lung diseases (CLDs) are a considerable source of morbidity and mortality and are thought to arise from dysregulation of normal wound healing processes. An aggressive, feature of many CLDs is pulmonary fibrosis (PF) and is characterized by excess deposition of extracellular matrix (ECM) proteins from myofibroblasts in airways. However, factors regulating myofibroblast biology are incompletely understood. Proteins in the cadherin family contribute epithelial to mesenchymal transition (EMT), a suggested source of myofibroblasts. Cadherin 11 (CDH11) contributes to developmental and pathologic processes that parallel those seen in PF and EMT. Utilizing Cdh11 knockout (Cdh11 -/-) mice, the goal of this …

Dialysis Communication Record, Paul Howells Rn,Bsn,Mba,Cnn, Susann R. Groller Rn, Bsn, Mba

Patient Care Services / Nursing

No abstract provided.

Pregnancy Outcomes In Female Liver Recipients Transplanted Under The Age Of 21, Carlo B. Ramirez, Warren R. Maley, Cataldo Doria, Lisa A. Coscia, Michael J. Moritz, Vincent T. Armenti

Department of Surgery Faculty Papers

Conclusions: Liver recipients transplanted under the age of 21 are able to maintain pregnancy with 75% resulting in a livebirth. Pregnancy, potential risks for mother and newborn, and long-term maternal survival should be discussed with the recipient and the parents of the recipient. Transplant centers are encouraged to report pregnancy outcomes in these recipients to the NTPR.

Contracting Of Primary Health Care Services In Pakistan: Is Up-Scaling A Pragmatic Thinking, Babar Shaikh, F. Rabbani, Najibullah Safi, Zia Dawar

Community Health Sciences

Quite often, public health care systems in developing countries are struggling because of incompetence and a lack of provider responsiveness to the needs of consumers. On the contrary, the private sector dominates the system of health provision. In recent years, contracting has been experimented as an approach to ensure delivery of comprehensive public health services in an efficient, effective, superior and fair manner and has generally thrived well. The state's healthcare system in Pakistan has suffered a lot, owing to structural fragmentation, resource scarcity, inefficiency and lack of functional specificity, gender insensitivity and inaccessibility. However, partnering with the private sector …

Breaking Bad News: Exploring Patient's Perspective And Expectations, Sidra Ishaque, Taimur Saleem, Fariha Batool Khawaja, Waris Qidwai

Department of Family Medicine

OBJECTIVE: To explore patient's perspectives and expectations from physicians with respect to breaking of bad news.

METHODS: A cross-sectional survey was carried out in the Community Health Centre of a tertiary care teaching hospital in Pakistan. All consenting individuals from 18 to 60 years of age were interviewed on the basis of a structured, pre-tested questionnaire.

RESULTS: The response rate for this study was 91.3%. A total of 400 respondents completed the full interview. About 60% patients had a fairly accurate idea about the implications of the phrase "bad news". A big proportion (44.1%) of people reported that bad news …

Forensic Aspects Of Unnatural Death In Children, K Jongprasartsuk, W. Charaschisri

Chulalongkorn Medical Journal

Objective : This study is a review of the causes of unnatural death among children for future prevention, statistics and medico-legal benefit. Setting : Department of Forensic Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok Study design : Descriptive study. Subjects and Methods : One hundred and one autopsy reports of unnatural death in children under 15 years old from 2001 to 2007 by forensic pathologists of the Department of Forensic Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok. Results : Sixty-eight were male and 33 were female. The most common age is ranged from 1 to 3 years old. Accident, especially …

Regulation Of Cyclin E1 By The Breast Cancer Microenvironment, Yuehan Wu

Biomedical Sciences ETDs

The behavior of breast epithelial cells is influenced by their microenvironment, which includes stromal cells and extracellular matrix. During breast cancer progression, the tissue microenvironment fails to control proliferation, resulting in uncontrolled growth and invasion. Upon invasion, the extracellular matrix encountered by breast cancer cells changes from primarily laminin and collagen IV to primarily collagen I. The current study used a three-dimensional (3D) collagen I culture model to address how the microenvironment controls the breast cancer cell cycle after invasion through the basement membrane. We show that culturing breast cancer cells in 3D collagen I inhibits proliferation through direct regulation …

Tactics For Identifying And Eliminating Tritrichomonas Foetus From Infected Beef Herds, Jeff D. Ondrak

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

The protozoan, Tritrichomonas foetus (TF), has been recognized as a cause of bovine infertility for more than 100 years (Skirrow and BonDurant, 1988). As an obligate parasite of the bovine reproductive tract its control and eradication seems achievable (Harding, 1950). However, this disease continues to trouble United States cattle producers and a recent epidemic in the Western US has lead to increased interest in research and regulatory efforts (Cima, 2009). Outbreak investigations were carried out on three Nebraska ranches to assess the efficiency of currently available diagnostic tests, culture, gel polymerase chain reaction (PCR), and real time PCR (rtPCR), in …

Socioeconomic Status And Prevalence Of Obesity And Diabetes In A Mexican American Community, Cameron County, Texas, 2004-2007, Susan P. Fisher-Hoch, Anne R. Rentfro, Jennifer Salinas, Adriana Perez, H. Shelton Brown, Belinda M. Reininger, Blanca I. Restrepo, Jeff Gaines Wilson, Monir Hossain, Mohammad H. Rahbar, Craig Hanis, Joseph B. Mccormick

School of Nursing Faculty Publications

Introduction Mexican Americans are at increased risk for obesity and diabetes. We established a cohort on the United States-Mexico border to determine the prevalence of obesity and diabetes in this Mexican American population and to see whether minor economic advantages had any effect on health.

Methods We randomly selected and extensively documented 810 people aged 35 to 64 years. Weighted data were analyzed to establish prevalence of obesity and diabetes and other markers of poor health such as elevated glycated hemoglobin levels.

Results Rates of obesity (body mass index ≥30 kg/m²) were 57% in the first (lower) of …

Implementation Of An Anthropomorphic Phantom For The Evaluation Of Proton Therapy Treatment Procedures, Ryan L. Grant

Dissertations and Theses (Open Access)

With an increasing number of institutions offering proton therapy, the number of multi-institutional clinical trials involving proton therapy will also increase in the coming years. The Radiological Physics Center monitors sites involved in clinical trials through the use of site visits and remote auditing with thermoluminescent dosimeters (TLD) and mailable anthropomorphic phantoms. Currently, there are no heterogeneous phantoms that have been commissioned to evaluate proton therapy. It was hypothesized that an anthropomorphic pelvis phantom can be designed to audit treatment procedures (patient simulation, treatment planning and treatment delivery) at proton facilities to confirm agreement between the measured dose and calculated …

Xenobiotic Metabolism Genes And Clubfoot, Amy M. Sommer

Dissertations and Theses (Open Access)

Idiopathic or isolated clubfoot is a common orthopedic birth defect that affects approximately 135,000 children worldwide. It is characterized by equinus, varus and adductus deformities of the ankle and foot. Correction of clubfoot involves months of serial manipulations, castings and bracing, with surgical correction needed in forty percent of cases. Multifactorial etiology has been suggested in numerous studies with both environmental and genetic factors playing an etiologic role. Maternal smoking during pregnancy is the only common environmental factor that has consistently been shown to increase the risk for clubfoot. Moreover, a positive family history of clubfoot and maternal smoking increases …

Self-Efficacy And Educational Interventions In Heart Failure: A Review Of The Literature, Karen S. Yehle, Kimberly Plake

School of Nursing Faculty Publications

Purpose: The purpose of this study was to conduct a systematic review of the literature to better understand how to structure educational interventions for heart failure patients to improve their self-efficacy for self-care behaviors.

Methods: A computer search of the literature from 1966 through May 2009 was conducted, yielding 84 manuscripts. All manuscripts were reviewed in relation to the search criteria, resulting in 12 articles that were evaluated.

Results: Each of the reviewed studies utilized an intervention plus education to improve self-efficacy. Self-efficacy was a primary outcome for seven of the studies, and a secondary outcome for five on the …

Operative Management Of Unstable Thoracolumbar Burst Fractures, Mujahid Jamil Khattak, Shakir Syed, Riaz Hussain Lakdawala

Department of Surgery Docs

Operative management of unstable burst vertebral fractures is challenging and debatable. This study of such cases was conducted at the Aga Khan Hospital, Karachi from January 1998 to April 2003. All surgically managed spine injuries were reviewed from case notes and operative records. Clinical outcome was assessed by Hanover spine score and correction of kyphosis was measured for radiological assessment. The results were analyzed by Wilcoxon sign rank test for two related samples and p-value < 0.05 was considered significant. Ten patients were identified by inclusion criteria. There was statistically significant difference between mean pre-and postoperative Hanover spine score (p=0.008). Likewise, there was significant difference between mean immediate postoperative and final follow-up kyphosis. (p=0.006). Critical assessment of neurologic and structural extent of injury, proper pre-operative planning and surgical expertise can optimize the outcome of patients

Acute Mesenteric, Portal And Inferior Vena Cava (Ivc) Venous Thrombosis: Optimal Outcome Achieved With Anticoagulation, Abdul Rehman Alvi, Shahida Bibi, Zia Ur Rehman, Sameullah Khan Niazi

Department of Surgery Docs

The prevalence and clinical spectrum of acute mesenteric venous thrombosis (AMVT) in Pakistan is largely unknown. The authors report two patients with acute mesenteric, portal and inferior vena cava venous thrombosis confirmed on CT imaging. The diagnoses were established within 24 hours of presentation and both patients were successfully treated with therapeutic heparin during hospital admission and continued on oral warfarin because of hypercoagulable state. The protocol that we currently use is evidence based and is leading to optimal outcome.

Unsaturated Fatty Acids Repress The Expression Of Adipose Fatty Acid-Binding Protein, Ap2, In Raw 264.7 Macrophage, Sara L. Coleman

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Adipocyte fatty acid binding protein, aP2, present in macrophages has been implicated in the integration of lipid metabolism and inflammatory response, contributing to development of insulin resistance and atherosclerosis. We investigated the modulation of aP2 expression by inflammatory insults and fatty acids in RAW 264.7 macrophages. When the cells were incubated with lipopolysaccharides (LPS; 100 ng/ml) or 10 ng/ml of tumor necrosis factor α for 18 h, aP2 mRNA and protein levels were drastically increased. Unsaturated fatty acids (100 μM of 18:1, 18:2, 18:3, 20:5 in complex with BSA), but not saturated fatty acids (16:0), significantly repressed the basal aP2 …

Youth School Enrichment Nutrition Programming In Nebraska: A Descriptive Study, Ashley M. Keller

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

The objectives of this study were to determine the nutrition knowledge of students in grades K-6 (n = 62,324) participating in Supplemental Nutrition Assistance Program-Education (SNAP-Ed), to determine if the nutrition information provided by the program impacts youth nutrition knowledge and behaviors, and to determine if there is a difference in the impact of the school enrichment program versus the non-school enrichment program. Without proper nutrition and physical activity the tendency for children to gain weight increases; therefore, increasing the likelihood of becoming obese. In the past 20 years, the prevalence of obesity has doubled. Eating behaviors and the amount …

Assessing Dietary Intake, Eating And Exercise Attitudes And Fitness Levels In College-Aged Students, Katie J. James

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

BACKGROUND The transition from high school to college can be a stressful lifestyle change for young adults. During this transition students develop a variety of health attitudes and behaviors that may have a negative impact on their overall wellbeing. OBJECTIVE To determine the correlation of BMI to dietary intake, fitness level and eating and exercise attitudes of college students. SETTING Participants completed an eating and exercise attitude survey, 2-day diet recall, and preformed three fitness tests at a Midwestern University through their Campus Recreational Center. PARTICIPANTS 772 students between the ages of 18-25 from a Midwestern University between the years …