Medicine and Health Sciences Commons^™

Structural Aberration In Ha Associated With Adaptation Of Human Influenza A H3n2 Virus In Embryonated Chicken Eggs, Viroj Wiwanitkit

Turkish Journal of Veterinary & Animal Sciences

Molecular changes associated with adaptation of human influenza A virus in embryonated chicken eggs are reported: 4 amino acid substitutions occurred in HA (G186V, S219F, V226I, V309I). These substitutions allowed binding to SAalpha2,3Gal- and SAalpha2,6Gal-containing receptors, conferred SAalpha2,3Gal specificity, and preserved antigenicity. Here, the author reports the result of protein secondary structure predictions of the adaption from its primary sequence using the NNPREDICT server. Comparing the classical HA of H3N2, the adaptation has additional helices and strands and these identified structural changes should be the explanation for the reported binding and antigenicity changes.

Nuclear Morphometry In Relation To Tumor Grade In Canine Spontaneous Cutaneous Squamous Cell Carcinomas, Radostin Simeonov

Turkish Journal of Veterinary & Animal Sciences

In a retrospective study on cytological specimens from 17 dogs with histologically confirmed cutaneous squamous cell carcinoma, the morphometric variables were studied and compared to tumors' nuclear grade. The morphometric parameters evaluated in this study were mean nuclear area (MNA, µm2), mean nuclear perimeter (MNP, µm), mean nuclear diameter (D mean, µm), minimum nuclear diameter (D min, µm), and maximum nuclear diameter (D max, µm). Associations between MNA, MNP, D mean, D min, and D max and tumors' grade were assessed using the ANOVA/LSD test (Statistica 6.0, StatSoft, USA) at a level of significance of P < 0.05. The result indicated an increase in the mean values of the nuclear parameters with increasing tumor grade. In the cases examined, significant differences in MNA, MNP, and D mean occurred between tumors in all grades. Besides, no significant differences in D max (between grade I-II and II-III) and D min (grade II-III) were found. In conclusion, nuclear morphometry could be used as an additional tool for differentiating nuclear grade in canine cutaneous squamous cell carcinomas.

Ileal Leiomyoma In A Captive Zebra (Equus Burchelli), Rahşan Yilmaz, Ahmet Akkoç, M. Özgür Özyi̇ği̇t

Turkish Journal of Veterinary & Animal Sciences

In the present study, an ileal leiomyoma encountered in a captive zebra (Equus burchelli) was reported. A 29-year-old, female zebra was found dead in the Bursa zoo. According to the referring veterinarian the most prominent clinical signs were intermittent colic (responsive to analgesics) and constipation. At post mortem examination, abdomen was severely distended. A firm, multilobular, well vascularized and encapsulated tumoural mass, 12 cm in diameter, embedded into the proximal part of the ileum wall, and protruded from the serosal surface was found. The cut section of the mass had whorled appearance, pale yellow to white in colour, and had …

The Pharmacogenomics Of Egfr-Dependent Nsclc: Predicting And Enhancing Response To Targeted Egfr Therapy, Justin M. Balko

University of Kentucky Doctoral Dissertations

The introduction of tyrosine kinase inhibitors (TKI) targeting the epidermal growth factor receptor (EGFR) inhibitors to the clinic has resulted in an improvement in the treatment of non small cell lung cancer (NSCLC). However, many patients treated with EGFR TKIs do not respond to therapy. The burden of failed treatment is largely placed on the healthcare field, limiting the effectiveness of EGFR TKIs. Furthermore, responses are hindered by the emergence of resistance. Thus, two questions must be addressed to achieve maximum benefit of EGFR inhibitors: How can patients who will benefit from EGFR TKIs be selected a priori? How …

The Interplay Between The Expression And Functions Of Wnt13 Isoforms During Apoptosis In Bovine Aortic Endothelial Cells, Tao Tang

University of Kentucky Doctoral Dissertations

Wnt proteins are crucial for development/homeostasis by controlling cell fate including apoptosis (Moon RT et al. 1997). Three humanWnt13 isoforms were identified: the secreted Wnt13A, mitochondrial Wnt13B, and nuclear Wnt13C forms; and nuclear Wnt13 had an increased sensitivity to LPS/TNF-induced apoptosis in primary endothelial cells (EC); both Wnt13B and C mRNA contain two start codons (AUG+1 and +74), but the same protein encoded from AUG+74 by Wnt13C was expressed lower than Wnt13B (Struewing IT et al.2006). We hypothesize that during EC apoptosis, the nuclear Wnt13C expression is regulated translationally; nuclear Wnt13 favors apoptosis through regulating the activity/expression of apoptosis-related factors; …

Post Discharge Nausea And Vomiting In Ambulatory Surgical Patients: Incidence And Management Strategies, Jan Odom Forren

University of Kentucky Doctoral Dissertations

Approximately 65% of all surgeries are conducted in the outpatient surgery setting involving more than 35 million patients. Thirty-five to fifty percent of these outpatients will experience post discharge nausea and vomiting (PDNV), nausea and vomiting that occurs after discharge from the health care facility after surgery. A dearth of literature details the problems associated with nausea and vomiting experienced by patients after discharge home from outpatient surgery.

The purposes of this dissertation were to (1) review the current knowledge in the area of post discharge nausea and vomiting; (2) present results of an integrative review of the research literature …

Treatment Options For Neuropathic Pain, Sara Akre

Honors Program Theses

Neuropathic pain is caused by injury to or disease of the nervous system. It affects many people around the world and is a symptom of several diseases. In the United States, it is estimated that up to 3% of Americans have neuropathic pain, which costs the healthcare system $40 billion annually (Gilron et al. 2006). It is, unfortunately, difficult to ameliorate or eliminate due to the complexity of the nervous system and its plasticity and is, therefore, a chronic condition. Health care professionals continually struggle to treat patients' pain and, since tolerance to painkillers is easily built up, this often …

Public Water Fluoridation, Jessica Fohey

Honors Program Theses

This thesis project addresses the issue of public water fluoridation. There is a debate within the United States as to whether or not public water systems should be fluoridated. Because of the large scale potential health impact, it is critical to determine whether or not this practice is in the best interest of the public. This paper provides an extensive review of scientific literature on the effectiveness, safety, and cost effectiveness of public water fluoridation. Following the review of scientific literature, I will present an overview of pseudo-scientific literature which advocates against public water fluoridation that, although often not scientifically …

Low Density Lipoprotein Receptor And Alzheimers Disease, Rangaraj K. Gopalraj

University of Kentucky Doctoral Dissertations

Since apoE allele status is the predominant Alzheimers disease (AD) genetic risk factor, functional single nucleotide polymorphisms (SNPs) in brain apoE receptors represent excellent candidates for association with AD. Therefore, three low density lipoprotein receptor (LDLR) SNPs were evaluated by TaqMan allelic discrimination assays for association with AD and I found that certain haplotypes alter the odds of AD. A SNP within LDLR exon 12, rs688, was identified in silico as neutralizing a putative exon splicing enhancer (ESE). Since LDLR is a major apoE receptor in the brain, I hypothesized that rs688 modulates LDLR splicing in neural tissues and associates …

Intra-Individual Variation In Children's Physical Activity Patterns: Implications For Measurement, Kate Ridley, Tim Olds, Beth P. Hands, Dawne Larkin, Helen Parker

Health Sciences Papers and Journal Articles

Children's physical activity (PA) patterns change form day to day. This intra-individual variability affects precision when measuring key physical activity and sedentary behavior variables. This paper discusses strategies to reduce the random error associated with intra-individual variability and demonstrates the implications for assessing PA when varying number of days are sampled. Self-reported data collected on two hundred and ninety eight 13- to 14-year-olds were used to compare estimates of PA and sedentary behaviour derived from between 1 and 7 days of recall data. Large intra-individual coefficients of variation were calculated for physical activity level (14.5%), moderate-to-vigorous physical activity (83.4%), screen …

The Self Reported Aggravating Activities Of Chronic Non Specific Low Back Pain Patients Do Not Demonstrate A Consistent Directional Pattern: An Observational Study, Benedict M. Wand, Rebecca Hunter, Neil E. O'Connell, Louise Marston, James Mcauley

Health Sciences Papers and Journal Articles

Question: Do the self-reported aggravating activities of chronic non-specific low back pain patients demonstrate a consistent directional pattern?

Design: Cross-sectional observational study. Participants: 240 chronic non specific low back pain patients.

Outcome measure: We invited experienced clinicians to classify each of the three self-nominated aggravating activities from the Patient Specific Functional Scale by the direction of lumbar spine movement. Patients were described as demonstrating a directional pattern if all nominated activities moved the spine into the same direction. Analyses were undertaken to determine if the proportion of patients demonstrating a directional pattern was greater than would be expected by chance. …

Fitness, Motor Competence And Body Composition Are Weakly Associated With Adolescent Back Pain, Mark Perry, Leon Straker, Peter O'Sullivan, Anne Smith, Beth P. Hands

Health Sciences Papers and Journal Articles

Study Design: Cross sectional

Objectives: To assess the associations between adolescent back pain and fitness, motor competence and body composition.

Background: Although deficits in physical fitness and motor control have been shown to relate to adult back pain, the evidence in adolescents is less clear.

Methods and measures: In this cross-sectional study, 1608 "Raine" cohort adolescents (mean age, 14 years) answered questions on lifetime, month and chronic prevalence of back pain, and participated in a range of physical tests assessing aerobic capacity, muscle performance, flexibility, motor competence, and body composition. A history of diagnosed back pain in the adolescent was …

Psychosocial Aspects Of Deafness: Implications For Rehabilitation Counselors, Diana M. Fraser, Sandra Hansmann, Shawn P. Saladin

School of Rehabilitation Services & Counseling Faculty Publications

Negative societal attitudes toward people with hearing loss can be handicapping. Because of their deafness, people face a multitude of external barriers which become their handicap. The primary barriers or handicaps to this underserved population are two fold, inaccessibility to appropriate services during important milestones in their lives and a misunderstanding of Deaf culture. Rehabilitation counselors are advised to become aware of the types of interventions their Deaf consumers had as children through their education and socialization for in sight to appropriate services. Also discussed is the importance of communication and employer involvement in reducing societal stereotypes.

A Survey Of Physical Therapists: Long Term Therapy Needs For Persons With Severe Disabilities, Irmo Marini, Karen Luckett, Eva Miller, E. Lisette Blanco

School of Rehabilitation Services & Counseling Faculty Publications

Sixty-three physical therapists participated in a survey designed to obtain their opinions regarding long-term and on-going future therapy recommendations for patients with spinal cord injuries, reflex sympathetic dystrophy, traumatic brain injuries, cerebral vascular accidents, cerebral palsy, and severe burns. Overall, therapists indicated these patients needed on-going therapy due to a loss or impairment of motor skills, ambulation skills, and loss or impairment of self-care skills. The primary reason reported for patients not returning to physical therapy for on-going treatment following their acute care was lack of insurance coverage or end of insurance benefits. This study outlines the implications for life …

Attitudes Of College Students Toward People With Disabilities, Wonsun (S.) Seo, Roy K. Chen

School of Rehabilitation Services & Counseling Faculty Publications

The current study sought to explore attitudinal differences in college students toward people with disabilities (PWD) based on their demographic backgrounds and levels of prior contact. Participants were 311 undergraduate and graduate students at a large public university in the Midwest. Using the Attitudes Towards Disabled Persons Scale, Form-O (Yuker & Block, 1986) and the Contact with Disabled Persons Scale (Yuker & Hurley, 1987), a moderate positive correlation between attitudes and levels of contact was found (r = .302, p < .001). Significant differences in the mean scores of the ATDP were observed for different demographic groups. A subsequent ANCOVA indicated that levels of prior contact were influential on attitudes towards PWD. Implications for rehabilitation practice and future research are discussed.

Are The Outcomes Of Immediate And Early Single Tooth Implants Comparable To Conventionally Placed Implants?, Gary L. Stafford

School of Dentistry Faculty Research and Publications

Data Sources

Medline, Embase, the Cochrane Central Register of Controlled Trials (Central) and references of relevant studies were searched.

Study selection

Longitudinal studies [randomised controlled trials (RCT), clinical trials, cohort-studies and case series] were considered for evaluation. Retrospective studies were excluded. Only case series that investigated at least five patients were considered for inclusion. Outcome measures considered were implant survival, radiographical assessment of marginal peri-implant bone levels, dental-professional-assessed aesthetics, peri-implant biological structures (level of marginal gingiva, papilla index, probing depth, presence of plaque, bleeding on probing), patient satisfaction, and biological and technical complications.

Data extraction and synthesis

Articles were screened …

Factors Affecting Occupational Therapists’ Decisions To Join Their State Association, Shawn Thomas Ross

Masters Theses

The following research focused on the factors affecting occupational therapists’ decisions to join, withdraw from, or never join their state association. Data was collected using a mixed methods design consisting of a survey that included quantitative questions and a qualitative question. The survey was sent to 1,908 occupational therapists in Michigan and 497 (26%) were returned and usable. Occupational therapists consistently reported dissatisfaction with their state association. Three themes developed regarding reasons occupational therapists’ decisions about membership: prioritization, interpersonal and group communication, and benefits. State and national association memberships were found to be associated. The greatest predictor of membership was …

Automatic Diagnosis For Prostate Cancer Using Run-Length Matrix Method, Xiaoyan Sun, Shao-Hui Chuang, Jiang Li, Frederic Mckenzie, Nico Karssemeijer (Ed.), Maryellen L. Giger (Ed.)

Electrical & Computer Engineering Faculty Publications

Prostate cancer is the most common type of cancer and the second leading cause of cancer death among men in US¹. Quantitative assessment of prostate histology provides potential automatic classification of prostate lesions and prediction of response to therapy. Traditionally, prostate cancer diagnosis is made by the analysis of prostate-specific antigen (PSA) levels and histopathological images of biopsy samples under microscopes. In this application, we utilize a texture analysis method based on the run-length matrix for identifying tissue abnormalities in prostate histology. A tissue sample was collected from a radical prostatectomy, H&E fixed, and assessed by a pathologist …

Efficacy And Safety Of Intermittent Preventive Treatment With Sulfadoxine-Pyrimethamine For Malaria In African Infants: A Pooled Analysis Of Six Randomised, Placebo-Controlled Trials, J.J. Aponte, D. Schellenberg, A. Egan, A. Breckenridge, I. Carneiro, J. Critchley, I. Danquah, A. Dodoo, R. Kobbe, B. Lell, J. May, Zul Premji, S. Sanz, E. Sevene, R. Soulaymani-Becheikh, P. Winstanley, S. Adjei, S. Anemana, D. Chandramohan, S. Issifou, F. Mockenhaupt F., S. Owusu-Agyei, B. Greenwood, M.P. Grobusch, P.G. Kremsner, E. Macete, H. Mshinda, R.D. Newman, L. Slutsker, M. Tanner, P. Alonso, C. Menendez

Pathology, East Africa

Background: Intermittent preventive treatment (IPT) is a promising strategy for malaria control in infants. We undertook a pooled analysis of the safety and efficacy of IPT in infants (IPTi) with sulfadoxine-pyrimethamine in Africa. Methods: We pooled data from six double-blind, randomised, placebo-controlled trials (undertaken one each in Tanzania, Mozambique, and Gabon, and three in Ghana) that assessed the efficacy of IPTi with sulfadoxine-pyrimethamine. In all trials, IPTi or placebo was given to infants at the time of routine vaccinations delivered by WHO's Expanded Program on Immunization. Data from the trials for incidence of clinical malaria, risk of anaemia (packed-cell volume <25% or haemoglobin <80 g/L), and incidence of hospital admissions and adverse events in infants up to 12 months of age were reanalysed by use of standard outcome definitions and time periods. Analysis was by modified intention to treat, including all infants who received at least one dose of IPTi or placebo. Findings: The six trials provided data for 7930 infants (IPTi, n=3958; placebo, n=3972). IPTi had a protective efficacy of 30·3% (95% CI 19·8-39·4, p<0·0001) against clinical malaria, 21·3% (8·2-32·5, p=0·002) against the risk of anaemia, 38·1% (12·5-56·2, p=0·007) against hospital admissions associated with malaria parasitaemia, and 22·9% (10·0-34·0, p=0·001) against all-cause hospital admissions. There were 56 deaths in the IPTi group compared with 53 in the placebo group (rate ratio 1·05, 95% CI 0·72-1·54, p=0·79). One death, judged as possibly related to IPTi because it occurred 19 days after a treatment dose, was subsequently attributed to probable sepsis. Four of 676 non-fatal hospital admissions in the IPTi group were deemed related to study treatment compared with five of 860 in the placebo group. None of three serious dermatological adverse events in the IPTi group were judged related to study treatment compared with one of 13 in the placebo group. Interpretation: IPTi with sulfadoxine-pyrimethamine was safe and efficacious across a range of malaria transmission settings, suggesting that this intervention is a useful contribution to malaria control. Funding: Bill & Melinda Gates Foundation. © 2009 Elsevier Ltd. All rights reserved.

Chlorproguanil - Dapsone - Artesunate Versus Artemether - Lumefantrine: A Randomized, Double-Blind Phase Iii Trial In African Children And Adolescents With Uncomplicated Plasmodium Falciparum Malaria, Zul Premji, R.E. Umeh, S. Owusu-Agyei, F. Esamai, E.U. Ezedinachi, S. Oguche, S. Borrmann, A. Sowunmi, S. Duparc, P.L. Kirby, A. Pamba, L. Kellam, R. Guiguemde, B. Greenwood, S.A. Ward, P.A. Winstanley

Pathology, East Africa

Background: Chlorproguanil-dapsone-artesunate (CDA) was developed as an affordable, simple, fixed-dose artemisinin-based combination therapy for use in Africa. This trial was a randomized parallel-group, double-blind, double-dummy study to compare CDA and artemether-lumefantrine (AL) efficacy in uncomplicated Plasmodium falciparum malaria and further define the CDA safety profile, particularly its hematological safety in glucose-6-phosphate dehydrogenase (G6PD) -deficient patients. Methods and Findings: The trial was conducted at medical centers at 11 sites in five African countries between June 2006 and August 2007. 1372 patients (≥1 to <15 years>old, median age 3 years) with acute uncomplicated P. falciparum malaria were randomized (2:1) to receive CDA 2/2.5/4 …

Coartem°: The Journey To The Clinic, Zul Premji

Pathology, East Africa

Artemisinin, from which the artemether component of Coartem° (artemether/lumefantrine, AL) is derived, is obtained from the plant sweet wormwood (Artemisia annua) which has been used for over 2,000 years as a Chinese herbal remedy. Artemisinin was first identified by Chinese researchers as the active anti-malarial constituent of A. annua and its derivatives were found to be the most potent of all anti-malarial drugs. Artemether acts rapidly, reducing the infecting parasite biomass by approximately 10,000-fold per asexual life cycle. Lumefantrine, the other active constituent of AL, acts over a longer period to eliminate the residual 100-100,000 parasites that remain after artemether …

Laryngeal Level Amplitude Modulation In Vibrato, Christopher Dromey, Lorie Reese, J. Arden Hopkin

Faculty Publications

Objectives: The goal of this investigation was to test a new methodology for measuring amplitude modulation (AM) at the level of the vocal folds during vibrato in trained singers, because previous research has suggested that AM arises in large part as an acoustic epiphenomenon through an interaction of the harmonics in the laryngeal source with the resonances of the vocal tract as the fundamental frequency oscillates.
Study Design: A within-subjects model was used to compare vocal activity across three pitch and three loudness conditions.
Methods: Seventeen female singers with a range of training and experience were recorded with a microphone …

Estimating Dysphonia Severity In Continuous Speech: Application Of A Multi-Parameter Spectral/Cepstral Model, Christopher Dromey, Shaheen N. Awan, Nelson Roy

Faculty Publications

The purpose of the study was to identify a sub-set of spectral/cepstral-based analysis methods that would most effectively predict dysphonia severity (as estimated via auditory-perceptual analysis) in samples of continuous speech. Acoustic estimates of dysphonia severity were used as an objective treatment outcomes measure in a set of pre- vs post-treatment speech samples. Pre- and post-treatment continuous speech samples from 104 females with primary muscle tension dysphonia (MTD) were rated by listeners using a 100 point visual analogue scale (VAS) and analysed acoustically with spectral/cepstral-based measures. Stepwise linear regression produced a three-factor model consisting of the cepstral peak prominence (CPP); …

A Motor Speech Programming Investigation Using Inhibition: Part I, Jeffrey Thomas Backlin

Dissertations and Theses @ UNI

The current study investigated stop/go boundaries for speech acts (i.e. saying "uh, annul, annulment") in a speech inhibition task modeled on Slater-Hammel's (1959) experiment. Specifically, the project sought to answer the following research questions: (1) Can human speakers learn to stop a sweep hand using their voice? (2) What is the absolute (100%) inhibition threshold for "uh?" (3) What is the threshold at which one half of the spoken response for "uh," can be inhibited? Two additional studies were conducted for the additional stop targets "annul" (Williams, 2009) and "annulment" (Corbett, 2009). Self-report and informal speech-language screening assessed ten adult …

A Speech Motor Programming Investigation Using Inhibition, Alison Michele Corbett

Dissertations and Theses @ UNI

Motor learning and its applications have been extensively studied, particularly in the area of sports physiology and kinesiology. From these fields, many theories of movement and its control mechanisms have evolved. Previous research has drawn upon these theatrical principles to investigate the specific parameters of skilled movements, specifically the time required to complete an action. In particular, a finger-lift task has been used to study the refractory period during which a motor program can not be inhibited when trained to occur at a particular temporal target (Slater-Hammel, 1959). The current study applies key methodological concepts from Slater-Hammel's work to examine …

Fiber’S Impact On High-Sensitivity C-Reactive Protein Levels In Cardiovascular Disease, Renea L. Beckstrand, Jennifer L. Butcher

Faculty Publications

The purpose of this study was to determine if increased dietary or supplemental intake of fiber slows or prevents inflammation as evidenced by hs-CRP values.

Morphoproteomic Evidence Of Constitutively Activated And Overexpressed Mtor Pathway In Cervical Squamous Carcinoma And High Grade Squamous Intraepithelial Lesions, Wei Feng, Xiuzhen Duan, Jinsong Liu, Jianguo Xiao, Robert E Brown

Faculty, Staff and Student Publications

Human papilloma virus (HPV) infection of the uterine cervix is linked to the pathogenesis of cervical cancer. Preclinical in vitro and in vivo studies using HPV-containing human cervical carcinoma cell lines have shown that the mammalian target of rapamycin (mTOR) inhibitor, rapamycin, and epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor, erlotinib, can induce growth delay of xenografts. Activation of Akt and mTOR are also observed in cervical squamous cell carcinoma and, the expression of phosphorylated mTOR was reported to serve as a marker to predict response to chemotherapy and survival of cervical cancer patients. Therefore, we investigated: a) the expression …

Implications For Nursing Practice: Delivering The Folic Acid Message, Kimberly Townsend Little

UNLV Theses, Dissertations, Professional Papers, and Capstones

In 1995, North Carolina (NC) had one of the highest prevalence rates of neural tube defects (NTDs) in the United States. Since the NC Folic Acid Council began focusing their efforts on educating women of childbearing age regarding NTDs in 1994, the prevalence of NTDs has declined overall by 40%; however, NTD prevalence among Hispanics in NC continues to be double the rate of non-Hispanics. Research has found daily consumption of a multivitamin with folic acid significantly decreases NTDs. Healthy People 2010 include in their objectives the need to increase folic acid consumption in women and reduce the NTD rates. …

Filipino Physician-Turned Nurses: A Phenomenological Study, Victor Rey Cui Vapor

UNLV Theses, Dissertations, Professional Papers, and Capstones

Foreign nurses have augmented the United States nursing workforce. The Philippines has remained the world's leading exporter of nurses, including the United States. More recently, a new phenomenon has emerged involving Filipino physicians who went back to school to take up nursing in the Philippines in order to migrate to foreign countries to work as nurses. The purpose of this study was to describe and to interpret the lived experiences of Filipino physician-turned nurses in the United States. Phenomenology was used as research design, with data obtained from a purposive sample of eight (8) self-identified physician-turned nurses in Las Vegas, …

Spectral Analysis Of Pathological Acoustic Speech Waveforms, Priyanka Medida

UNLV Theses, Dissertations, Professional Papers, and Capstones

Biomedical engineering is the application of engineering principles and techniques to the medical field. The design and problem solving skills of engineering are combined with medical and biological science, which improves medical disorder diagnosis and treatment. The purpose of this study is to develop an automated procedure for detecting excessive jitter in speech signals, which is useful for differentiating normal from pathologic speech. The fundamental motivation for this research is that tools are needed by speech pathologists and laryngologists for use in the early detection and treatment of laryngeal disorders. Acoustical analysis of speech was performed to analyze various features …