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Full-Text Articles in Medicine and Health Sciences

Light And Scanning Electron Microscopic Studies On The Lingual Papillae Of 80-Day-Old Wild Pig Fetal Siblings, Sevi̇nç Ateş, Yeşi̇m Akaydin Bozkurt, Tolunay Kozlu, Aydin Alan, Ayhan Düzler Jan 2013

Light And Scanning Electron Microscopic Studies On The Lingual Papillae Of 80-Day-Old Wild Pig Fetal Siblings, Sevi̇nç Ateş, Yeşi̇m Akaydin Bozkurt, Tolunay Kozlu, Aydin Alan, Ayhan Düzler

Turkish Journal of Veterinary & Animal Sciences

This study examined the lingual papillae of 80-day-old wild pig fetal siblings through macroscopic and light and scanning electron microscopic observations. The results revealed that fungiform papillae were located on every aspect of the tongue except the ventral surface and the center of the dorsal surface. Conical papillae were present on the dorsal surface of the root of the tongue and marginal papillae were observed on the lateral edges of the tip of the tongue. There were 2 vallate papillae on the dorsal and 2 foliate papillae on the lateral aspects of the root of the tongue. The filiform papillae …


A Macroanatomical Study Of The Arcus Aortae In The Fox (Vulpes Vulpes), Emi̇ne Karakurum, Özcan Özgel Jan 2013

A Macroanatomical Study Of The Arcus Aortae In The Fox (Vulpes Vulpes), Emi̇ne Karakurum, Özcan Özgel

Turkish Journal of Veterinary & Animal Sciences

The present study was aimed at the investigation of the anatomy of the arcus aortae in 5 adult female foxes. It was determined that 2 main arteries, namely the truncus brachiocephalicus and arteria subclavia sinistra, originated from the fifth intercostal space at the arcus aortae. It was ascertained that, firstly, the arteria carotis communis sinistra stemmed from the truncus brachiocephalicus, and the continuation of the artery bifurcated into the arteria subclavia dextra and arteria carotis communis dextra. The first branch, which had its origin at the arteria subclavia sinistra, was the arteria vertebralis. Further along its course, the artery gave …


Prevalence Of Shiga Toxin-Producing Escherichia Coli And Pathogenic Leptospira Spp. In Rodents From Outdoor Farms In Western Spain, Alfredo Garcia, Remigio Martinez, Lourdes Garcia, Jose Manuel Benitez-Medina, David Risco, Waldo Luis Garcia, Joaquin Rey, Juan Manuel Alonso, Angeli Kodjo Jan 2013

Prevalence Of Shiga Toxin-Producing Escherichia Coli And Pathogenic Leptospira Spp. In Rodents From Outdoor Farms In Western Spain, Alfredo Garcia, Remigio Martinez, Lourdes Garcia, Jose Manuel Benitez-Medina, David Risco, Waldo Luis Garcia, Joaquin Rey, Juan Manuel Alonso, Angeli Kodjo

Turkish Journal of Veterinary & Animal Sciences

Sixty-five rats (Rattus norvegicus) and 5 mice (Mus musculus) were trapped from 8 outdoor farms and the presence of Shiga toxin-producing Escherichia coli was not detected in any individual. Nevertheless, using the microscopic agglutination test, it was found that 20% (13/65) of rat serum samples were positive for anti-Leptospira antibodies, and DNA of pathogenic leptospires was detected in 3.07% (2/65) of the rat kidneys by real-time polymerase chain reaction. The most common infecting serovar was Castellonis (7.69%), followed by Canicola (6.15%) and Mangus (6.15%). According to these results, rats from outdoor farms may be considered a reservoir host for Leptospira …


Prevention Of Embryonic Death Using Different Hormonal Treatments In Ewes, Mehmet Bozkurt Ataman, Mehmet Aköz, Mustafa Kemal Saribay, Hüseyi̇n Erdem, Mustafa Numan Bucak Jan 2013

Prevention Of Embryonic Death Using Different Hormonal Treatments In Ewes, Mehmet Bozkurt Ataman, Mehmet Aköz, Mustafa Kemal Saribay, Hüseyi̇n Erdem, Mustafa Numan Bucak

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to evaluate different treatment protocols to prevent embryonic death in ewes. A total of 180 Akkaraman crossbred ewes and 10 healthy rams were used as material. The ewes were divided into 3 equal groups, with each of the 3 groups then separated into 3 subgroups. Ewes in estrus, determined with teaser rams, were exposed to mating. Three different treatment protocols of gonadotropin-releasing hormone (GnRH) analog (buserelin, intramuscularly) at a dose of 20 µg, vaginal sponges containing 30 mg fluorogestone acetate (FGA), and saline at a dose of 1 mL (control, intramuscularly) were applied on …


Effects Of Feeding Dry Fat And Yeast Culture On Broiler Chicken Performance, Mutassim Mohamed Abdelrahman Jan 2013

Effects Of Feeding Dry Fat And Yeast Culture On Broiler Chicken Performance, Mutassim Mohamed Abdelrahman

Turkish Journal of Veterinary & Animal Sciences

A feeding trial with 400 male broilers (Ross) was conducted to investigate the effect of using dry fat and 2 levels of yeast culture (Saccharomyces cerevisiae) on performance, blood glucose, cholesterol, calcium, phosphorus, cobalt, copper, magnesium, manganese, and zinc. One-day-old broilers were distributed among 4 treatments, with 4 replicates and 25 chicks/replicate. The treatments were: diet 1 (basal diet with maize oil, group T1), diet 2 (basal diet with dry fat, group T2), diet 3 (basal diet with dry fat + 2 kg of yeast culture/t diet, group T3), and diet 4 (basal diet with dry fat + 3 kg …


Seroepidemiology Of Canine Brucellosis In Beijing, China, Fu Xiang, Zhaofei Xia, Qingming Wu, Yanyun Chen, Jinhau Yu, Jianging Wan Jan 2013

Seroepidemiology Of Canine Brucellosis In Beijing, China, Fu Xiang, Zhaofei Xia, Qingming Wu, Yanyun Chen, Jinhau Yu, Jianging Wan

Turkish Journal of Veterinary & Animal Sciences

A seroepidemiological survey of canine brucellosis was carried out in Beijing, China. Serum samples were taken from dogs admitted to the Beijing Companion Animal Hospital for immigration and emigration inspection, including dogs from 4 breeding farms and stray dogs (n = 1200, 14 districts), between January 2008 and April 2009. All of the sera were first tested and preselected by Rose Bengal plate agglutination test (RBAT), and the positive samples by RBAT were determined by tube agglutination test (TAT) again. Of the 1200 dogs studied, 60 (5%) were positive by RBAT, 21 (51.7%) of which were confirmed by TAT. The …


Changes In The Bioactive Protein Concentrations In The Bovine Colostrum Of Jersey And Polish Holstein–Friesian Cows, Monika Sobczuk-Szul, Zofia Wielgosz-Groth, Marek Wronski, Arkadiusz Rzemieniewski Jan 2013

Changes In The Bioactive Protein Concentrations In The Bovine Colostrum Of Jersey And Polish Holstein–Friesian Cows, Monika Sobczuk-Szul, Zofia Wielgosz-Groth, Marek Wronski, Arkadiusz Rzemieniewski

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine changes in the bioactive protein concentrations in the bovine colostrum of Jersey and Polish Holstein-Friesian (PHF) cows. Double colostrum samples were collected from 16 Jersey and PHF cows, during 8 successive milking runs after calving. A total of 256 colostrum samples were analyzed to determine the pH, density, percentage share, and content of selected bioactive proteins, including the percentage share of casein fractions (\alpha_s-, \beta-, \gamma-, and \kappa-) in the total casein, and the concentrations of \alpha-lactalbumin (\alpha-LA), \beta-lactoglobulin (\beta-LG), lactoferrin (LF), interleukin-1\beta, interleukin-6, and tumor necrosis factor-alpha. The concentrations of the …


A Retrospective Study Of Tetralogy Of Fallot In Dogs, Urszula Paslawska, Agnieszka Noszczyk-Nowak, Alicja Cepiel, Maciej Staszczyk, Adrian Janiszewski Jan 2013

A Retrospective Study Of Tetralogy Of Fallot In Dogs, Urszula Paslawska, Agnieszka Noszczyk-Nowak, Alicja Cepiel, Maciej Staszczyk, Adrian Janiszewski

Turkish Journal of Veterinary & Animal Sciences

Tetralogy of Fallot is a rare congenital heart defect; however, it is the most common cyanotic heart malformation in humans and animals. It consists of a ventricular septal defect, pulmonic stenosis, displacement of the aortic root, and hypertrophy of the right ventricle. At the Department of Internal Medicine Clinic of Diseases of Horses, Dogs, and Cats at the Wroclaw University of Environmental and Life Sciences, patients with Fallot syndrome constituted 4.44% of all patients with congenital heart diseases. There was no breed predisposition found. The most common clinical signs were exercise intolerance, dyspnea, and cyanosis of the mucous membranes, but …


Determination Of Newcastle Disease Virus Among Wild Bird Populations In Lake Van Basin, Turkey, Banur Boynukara, Ti̇mur Gülhan, Fethi̇ye Çöven, İlhami̇ Ki̇zi̇roğlu, Ati̇lla Durmuş Jan 2013

Determination Of Newcastle Disease Virus Among Wild Bird Populations In Lake Van Basin, Turkey, Banur Boynukara, Ti̇mur Gülhan, Fethi̇ye Çöven, İlhami̇ Ki̇zi̇roğlu, Ati̇lla Durmuş

Turkish Journal of Veterinary & Animal Sciences

In this study, 540 feces samples taken from a total of 22 bird species, including transit migrant, winter visitor, migratory, and resident bird species, in Lake Van basin were examined by real-time polymerase chain reaction (RT-PCR) with respect to the Newcastle disease virus (NDV). All of the feces samples were cultured in specific pathogen-free embryonated chicken eggs (ECEs) for NDV isolation. The NDV isolates were analyzed for virulent fusion (F) protein by RT reverse transcription PCR (RRT-PCR). Of the 540 examined feces samples, 28 (5.18%) were found to be positive by RT-PCR. The same samples were cultured in ECEs and, …


Effects Of Parity And Litter Size On The Energy Contents And Immunoglobulin G Concentrations Of Awassi Ewe Colostrum, Shogo Higaki, Masashi Nagano, Seiji Katagiri, Yoshiyuki Takahashi Jan 2013

Effects Of Parity And Litter Size On The Energy Contents And Immunoglobulin G Concentrations Of Awassi Ewe Colostrum, Shogo Higaki, Masashi Nagano, Seiji Katagiri, Yoshiyuki Takahashi

Turkish Journal of Veterinary & Animal Sciences

This study determined the energy content and immunoglobulin G (IgG) concentration of Awassi ewe colostrum and examined the effects of parity and litter size on them. Colostral samples were collected from 57 ewes from the 1st to 7th parities with a litter size of 1 or 2, within 12 h after lambing. Concentrations of colostral fat, lactose, and protein were measured as energy sources, and IgG was measured as representative of the immunoglobulin content of the colostrum. Mean values obtained from analyses of the ewe colostrum samples were 7.4 ± 2.1 kJ/g for the energy value and 60.9 ± 21.4 …


Multiple Jaw Fractures And Hiatus Linguae In A Dog, İbrahi̇m Akin, Murat Sarierler, Nuh Kiliç, Ali̇ Belge Jan 2013

Multiple Jaw Fractures And Hiatus Linguae In A Dog, İbrahi̇m Akin, Murat Sarierler, Nuh Kiliç, Ali̇ Belge

Turkish Journal of Veterinary & Animal Sciences

The case of a 3.5-year-old male Norwegian Elkhound crossbreed dog, seen the day after a traffic accident, is reported. Fractures in the symphysis mandibulae and the right corpus mandibulae were diagnosed. The apex linguae hung down laterally; a 2-cm cut wound was present on the right side. Two more cut wounds (6 cm long on the ventral surface and 3 cm long on the dorsal aspect) of the tongue in a direction oblique to its long axis were connected by a cut through the entire thickness of the tongue. Tooth 103 was broken at the gingival line and 104 under …


Cytotoxicity Of Nickel Ions For Human Osteoblasts In The Context Of Orthodontic Treatment In Humans And Animals, Marcin Mikulewicz, Katarzyna Chojnacka, Iwona Kochanowska, Agnieszka Dziewicka, Maciej Janeczek, Jerzy Cieplik, Aleksander Chrószcz Jan 2013

Cytotoxicity Of Nickel Ions For Human Osteoblasts In The Context Of Orthodontic Treatment In Humans And Animals, Marcin Mikulewicz, Katarzyna Chojnacka, Iwona Kochanowska, Agnieszka Dziewicka, Maciej Janeczek, Jerzy Cieplik, Aleksander Chrószcz

Turkish Journal of Veterinary & Animal Sciences

In the present work, the biocompatibility of orthodontic devices was tested by the propagation of human osteoblasts. A different methodological approach was applied. Since the materials used in human and veterinary orthodontics (not in implantology) had been tested, osteoblasts were not implanted on the surface of the biomaterial, but have been cultured in the medium containing the products of corrosion. In the present work, osteoblasts were propagated in the medium spiked with Ni(II) salt versus the control. Osteoblast cells (live, dead, and total) were counted under light microscope. The statistical significance of the differences between the experimental and control groups …


The Use Of Progesterone-Supplemented Co-Synch And Ovsynch For Estrus Synchronization And Fixed-Time Insemination In Nulliparous Saanen Goat, Zekari̇ya Nur, Yavuz Nak, Deni̇z Nak, Burcu Üstüner, Bi̇lgi̇ner Tuna, Gözde Şi̇mşek, Hakan Sağirkaya Jan 2013

The Use Of Progesterone-Supplemented Co-Synch And Ovsynch For Estrus Synchronization And Fixed-Time Insemination In Nulliparous Saanen Goat, Zekari̇ya Nur, Yavuz Nak, Deni̇z Nak, Burcu Üstüner, Bi̇lgi̇ner Tuna, Gözde Şi̇mşek, Hakan Sağirkaya

Turkish Journal of Veterinary & Animal Sciences

The effect of Chronogest-supplemented Ovsynch and Co-synch protocols on estrus synchronization, follicular development, and fertility after fixed-time insemination was investigated in goats during the breeding season. Co-synch (n = 24) and Ovsynch (n = 25) protocols were applied to nulliparous does with a body weight of more than 30 kg. The onset and duration of estrus were determined with teaser bucks. The does were laparoscopically inseminated with 11.5 × 10^6 motile spermatozoa/straw at the second gonadotropin-releasing hormone (GnRH) injection for Co-synch and 8 h after the second GnRH injection for Ovsynch. The rates of estrus exhibited in the does were …


Investigation Of The Effects Of Co2 Insufflation On Blood Gas Values During Laparoscopic Procedures In Pigs, Özlem Güzel, Di̇lek Olğun Erdi̇kmen, Di̇dar Aydin, Zi̇hni̇ Mutlu, Esma Yildar Jan 2012

Investigation Of The Effects Of Co2 Insufflation On Blood Gas Values During Laparoscopic Procedures In Pigs, Özlem Güzel, Di̇lek Olğun Erdi̇kmen, Di̇dar Aydin, Zi̇hni̇ Mutlu, Esma Yildar

Turkish Journal of Veterinary & Animal Sciences

Due to the fact that they produce less post-operative pain and shorter hospitalisation times, laparoscopic surgery procedures are more widely used than many traditional open surgical methods. However, the carbon dioxide gas used to visualise the surgical site during the procedure causes complications such as an increase in the blood CO_2 level and acidosis. In the present study, 12 male pigs were used. Sedation was achieved via intramuscular injection of xylazine at a dose of 2 mg/kg and anaesthesia induction was done via slow intravenous injection of ketamine HCl at a dose of 2 mg/kg. Following endotracheal intubation, general anaesthesia …


A Microscopic And Stereological Study Of The Renal Artery Transitional Zone Of The Adult Male Dog, Fatemah Ramezani Nowrozani Jan 2012

A Microscopic And Stereological Study Of The Renal Artery Transitional Zone Of The Adult Male Dog, Fatemah Ramezani Nowrozani

Turkish Journal of Veterinary & Animal Sciences

A transitional zone in the dog renal artery was documented and its parameters were recorded: length and volumes of the luminal wall, tunica intima, tunica media, and tunica adventitia-as well as these parameters in the non-transitional zone of artery. Twelve normal adult male dogs were studied. The specimens were processed in the routine manner for light and electron microscopy. After the determination of transitional zone length by light microscope, for the stereological study each renal artery was divided into transitional and non-transitional zones, and paraffin blocks were prepared. It was established that the transitional zone contains parallel and continuous elastic …


Isospora Species (I. Canaria, Isospora Sp.) In Canaries (Serinus Canarius, Linnaeus), Cem Ecmel Şaki̇, Edi̇p Özer Jan 2012

Isospora Species (I. Canaria, Isospora Sp.) In Canaries (Serinus Canarius, Linnaeus), Cem Ecmel Şaki̇, Edi̇p Özer

Turkish Journal of Veterinary & Animal Sciences

Of 64 canaries with diarrhea in Elazığ Province between May 2002 and May 2006, 18 (28.1%) had Isospora oocysts in their feces. It was determined that the oocysts, examined morphologically before and after sporulating, were Isospora canaria and Isospora sp. Among the infected animals, 8 (44.4%) were concurrently infected with both species. Total numbers of I. canaria oocysts were greater than those of Isospora sp. A total of 2 canaries showing severe clinical signs were necropsied. Liver, spleen, and lung preparations and peripheral blood smears of the necropsied animals were examined for Atoxoplasma, and no development forms were seen. For …


Prolonged Dystocia, Uterine Necrosis, And Ovariohysterectomy In A Chios Ewe, Christos N. Brozos, Lazaros Lazarides, Isidoros Karagiannis, Evangelos Kiossis, Georgios Tsousis, Vasilios Psychas, Nektarios Giadinis Jan 2012

Prolonged Dystocia, Uterine Necrosis, And Ovariohysterectomy In A Chios Ewe, Christos N. Brozos, Lazaros Lazarides, Isidoros Karagiannis, Evangelos Kiossis, Georgios Tsousis, Vasilios Psychas, Nektarios Giadinis

Turkish Journal of Veterinary & Animal Sciences

A 4-year-old Chios ewe was submitted to the Clinic of Farm Animals (School of Veterinary Medicine, Aristotle University of Thessaloniki). The animal had delivered 2 healthy lambs, 2 days earlier, and was inappetent, depressed, and reluctant to move, while it had tachycardia and tachypnea. The cervix was opened at a dilation of 1 finger in diameter and fetid fetal membranes expelled from the vagina. Serum biochemistry revealed renal and liver disease, while ultrasonography revealed uterine dilatation. Laparotomy revealed a partly autolysed embryo in the uterus and gangrenous metritis. Clostridium perfringens and Escherichia coli were isolated in bacterial cultures from the …


Morphological Characterization Of The Immune Organs In Ostrich Chicks, Hui Song, Ke-Mei Peng, Sheng-He Li, Yan Wang, Lan Wei, Li Tang Jan 2012

Morphological Characterization Of The Immune Organs In Ostrich Chicks, Hui Song, Ke-Mei Peng, Sheng-He Li, Yan Wang, Lan Wei, Li Tang

Turkish Journal of Veterinary & Animal Sciences

This study describes the anatomical, light microscopic, and ultrastructural characterization of the immune organs in ostrich chicks. In this study 6 healthy 8-week-old ostrich chicks were processed for light and transmission electron microscopy. Hematoxylin and eosin as well as improved Weigert staining were used for light microscopy. The small thymus, which clustered caudally along both sides of the neck, contained Hassall's corpuscles in the medulla. The cloacal bursa, which formed the hanging and lateral walls of the cloaca, enveloped the cloaca but did not form a truly independent bursa. Inside the bursa convex papillae were densely distributed on the surface …


Optimum Temperature And Growth Performance Of Hatchery Reared Black Sea Flounder (Platichthys Flesus Luscus Pallas, 1814), İlhan Aydin, Orhan Ak, Ercan Küçük, Hamza Polat, Bi̇nnur Ceylan Jan 2012

Optimum Temperature And Growth Performance Of Hatchery Reared Black Sea Flounder (Platichthys Flesus Luscus Pallas, 1814), İlhan Aydin, Orhan Ak, Ercan Küçük, Hamza Polat, Bi̇nnur Ceylan

Turkish Journal of Veterinary & Animal Sciences

The present study focused on the effect of water temperature on growth and feed conversion ratio of juvenile Black Sea flounder (Platichthys flesus luscus; initial weight: 2.0 g ± 0.01 g). The experiment was carried out for 84 days with the following range of water temperatures: 13, 17, 21, and 25 °C. Growth, food consumption, and feed conversion ratio were the highest at 21 °C and the lowest at 13 °C. The growth rate of the fish increased with water temperatures up to 21 °C and it decreased significantly with a further increase in temperature. The conclusion of this study …


Full Length Cdna Synthesis Of Differentially Displayed Ests During Lactation In The Indian Buffalo (Bubalus Bubalis), Ajai Tripathi, Krishna M. Singh, Makrand N. Vaze, Umed V. Ramani, Dharamsi N. Rank, Chaitanya G. Joshi Jan 2012

Full Length Cdna Synthesis Of Differentially Displayed Ests During Lactation In The Indian Buffalo (Bubalus Bubalis), Ajai Tripathi, Krishna M. Singh, Makrand N. Vaze, Umed V. Ramani, Dharamsi N. Rank, Chaitanya G. Joshi

Turkish Journal of Veterinary & Animal Sciences

In the present study, antisense primers were designed from 7 differentially displayed expressed sequence tags (ESTs) previously identified at our laboratory. Total RNA was isolated from the mammary gland tissue of lactating buffalo to carry out 5' rapid amplification cDNA ends (RACE) PCR. 5' RACE PCR amplicons, ranging from 161 bp to 336 bp, were cloned and sequenced. These 5' RACE sequences were analyzed using the blastn (Megablast) bioinformatics tool available from NCBI. Of these 7, only one 5' mRNA sequence did not show a match in the available database and another 1 failed to show a match with any …


Seasonal And Group Effects On Dairy Cow Behavior In Large Yards, Akbar Nikkhah, Rasool Kowsar Jan 2012

Seasonal And Group Effects On Dairy Cow Behavior In Large Yards, Akbar Nikkhah, Rasool Kowsar

Turkish Journal of Veterinary & Animal Sciences

In mechanized modern dairy facilities with competitive environments, monitoring behavior provides opportunities to manipulate and optimize the nutritional, health, and social status of high-merit cows. The objective of the current study was to determine seasonal and cow group effects on the eating, ruminating, standing, and lying behaviors of dairy cows in large yards. Seasonal data on various behaviors of lactating cows in different production and lactation stages were collected continuously for 26 months, from December 2006 through February 2008. The herd had approximately 3000 dairy cattle housed in groups within specific yards. A total of 415 multiparous high-producing cows (MH), …


A Retrospective Study Of Anaplasma In Minnesota Cattle, Aneela Durrani, Sagar Goyal Jan 2012

A Retrospective Study Of Anaplasma In Minnesota Cattle, Aneela Durrani, Sagar Goyal

Turkish Journal of Veterinary & Animal Sciences

To determine the seroprevalence of Anaplasma in Minnesota cattle, the database of the Veterinary Diagnostic Laboratory (VDL), University of Minnesota was searched over a 10 year period (2001 to July 2010). A total of 438,407 bovine serum samples were tested by complement fixation test (CFT) and competitive enzyme linked immunosorbent test (cELISA). The positive rate of seroprevalence by CFT was 2.4% out of 211,484 samples, with a 0.13% anticomplementary result. The VDL at the University of Minnesota started using cELISA after 2002, and CFT application was suspended as diagnostic test for anaplasmosis after 2005 due to its complexity. With cELISA, …


Effect Of Fermented Protein Feedstuffs On Pig Production Performance, Nutrient Digestibility, And Fecal Microbes, Juan Chang, Qingqiang Yin, Pengpeng Wang, Weimin Wang, Ruiyu Zuo, Qiuhong Zheng, Junxi Liu Jan 2012

Effect Of Fermented Protein Feedstuffs On Pig Production Performance, Nutrient Digestibility, And Fecal Microbes, Juan Chang, Qingqiang Yin, Pengpeng Wang, Weimin Wang, Ruiyu Zuo, Qiuhong Zheng, Junxi Liu

Turkish Journal of Veterinary & Animal Sciences

In order to determine the availability of fermented protein feedstuffs (FPFS)-such as cottonseed, blood, and feather meals—with Aspergillus oryzae, 2 phases of feeding experiments were adopted for 7 weeks. Sixty 58-day-old crossbred pigs were assigned to 6 groups, 10 pigs per group. Group 1 was the control; groups 2, 3, and 4 were supplemented with 6%-7%, 12%-14%, and 18%-21% FPFS, respectively, by replacing the same percentage of soybean meal (SBM); group 5 was supplemented with 12%-14% unfermented protein feedstuffs (UFPFS) by replacing the same percentage of SBM; group 6 was on the same base as group 3 with digestive energy …


Detection, Cloning, And Sequencing Of The Enterotoxin Gene Of Clostridium Perfringens Type C Isolated From Goat, Yuan Tang, Ming Wen, Bin Lian, Jianzhong Cheng, Kaigong Wang, Bijun Zhou Jan 2012

Detection, Cloning, And Sequencing Of The Enterotoxin Gene Of Clostridium Perfringens Type C Isolated From Goat, Yuan Tang, Ming Wen, Bin Lian, Jianzhong Cheng, Kaigong Wang, Bijun Zhou

Turkish Journal of Veterinary & Animal Sciences

Clostridium perfringens enterotoxin (CPE) is an important virulence factor involved in C. perfringens food poisoning and other gastrointestinal illnesses. In our experiment a Clostridium perfringens enterotoxin gene (cpe) positive type C strain was obtained by multiplex PCR from 34 C. perfringens isolates from goat in Guizhou Province, China. Then a pair of specific primers was designed and synthesized according to the published cpe gene, and the complete cpe gene was amplified from the genomic DNA of the cpe-positive C. perfringens isolate. The PCR product was cloned into pMD18-T vectors and transformed into competent cell DH5a. The recombinant plasmid was identified …


The Effect Of Bleeding On Peripheral Blood And Head Kidney Hematopoietic Tissue In Common Carp (Cyprinus Carpio), Elzbieta Kondera, Aneta Dmowska, Monika Rosa, Malgorzata Witeska Jan 2012

The Effect Of Bleeding On Peripheral Blood And Head Kidney Hematopoietic Tissue In Common Carp (Cyprinus Carpio), Elzbieta Kondera, Aneta Dmowska, Monika Rosa, Malgorzata Witeska

Turkish Journal of Veterinary & Animal Sciences

Adverse environmental conditions (contamination of water with nitrite, pesticides, or other pollutants), nutritional deficiencies, bacterial and viral infections, and parasitic invasions may induce anemia in fish, in both natural and aquaculture conditions. Changes in hematological values and cellular composition of peripheral blood and head kidney hematopoietic tissue of bled common carp (Cyprinus carpio L.) were evaluated over a 4 week period post-bleeding (30% blood loss). The results showed that fish very quickly compensated for anemia. Erythrocyte count (RBC), hemoglobin concentration (Hb), and hematocrit (Ht) gradually increased to values significantly higher than they were before bleeding; this was accompanied by an …


The Effect Of Different Extenders On The Motility And Morphology Of Ram Sperm Frozen Or Stored At 4 °C, Recai̇ Kulaksiz, Çi̇ğdem Çebi̇, Ergün Akçay Jan 2012

The Effect Of Different Extenders On The Motility And Morphology Of Ram Sperm Frozen Or Stored At 4 °C, Recai̇ Kulaksiz, Çi̇ğdem Çebi̇, Ergün Akçay

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to evaluate the effects on sperm motility and abnormality in ram semen extended with skimmed milk (M), sodium citrate (SC), Tris (T), and Bioxcell® (B) after storage in liquid form at 4 °C and in frozen form. Ejaculates were collected from 3 Akkaraman rams by artificial vagina twice a week during the non-breeding season. After pooling, each pooled ejaculate was split into 4 equal aliquots and diluted with skimmed milk (M), sodium citrate (SC), Tris (T), and Bioxcell® (B) extenders. Sperm motility was significantly higher (P < 0.05) in M compared with B, SC, and T on the first and second days (48 h) of storage. With regard to the percentage of total abnormal spermatozoa, the results in M were different from (P < 0.05) from those of SC and T on the first day (24 h) of storage but no different from B. Differences among extenders were found to be significant post-thawing for spermatozoa motility and the percentage of total abnormal spermatozoa. The total abnormality of semen diluted with M was significantly lower than that observed in the other extenders. Consequently, it was found that skimmed milk was better than the other extenders in terms of the sperm parameters evaluated during liquid storage and post-thaw.


Dust And Endotoxin In Laying Hen Dwellings, Kristina Matkovic, Marija Vucemilo, Bara Vinkovic Jan 2012

Dust And Endotoxin In Laying Hen Dwellings, Kristina Matkovic, Marija Vucemilo, Bara Vinkovic

Turkish Journal of Veterinary & Animal Sciences

Only healthy laying hens are capable of producing an average of 280 eggs in 1 year of exploitation. Production of consume eggs takes place in intensive housing systems where the laying hens are still mainly kept in cages under controlled conditions. Technical infrastructure and hygiene quality do not guarantee a production atmosphere completely free from pollutants, including potential pathological risk factors. Laying hens and farm workers are exposed to large quantities of bioaerosols in henhouses. Bioaerosols represent a mixture of different biological particles which can, under certain circumstances, cause health and welfare problems in animals. The most important components are …


Reproductive Performance Of Primiparous And Multiparous Saanen Goats After Laparoscopic Intrauterine Insemination: A Field Study, Recai̇ Kulaksiz, Ali̇ Daşkin Jan 2012

Reproductive Performance Of Primiparous And Multiparous Saanen Goats After Laparoscopic Intrauterine Insemination: A Field Study, Recai̇ Kulaksiz, Ali̇ Daşkin

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to evaluate the reproductive performance of primiparous and multiparous Saanen goats after intrauterine laparoscopic artificial insemination with frozen semen. Twenty-four Saanen goats, divided in 2 groups: group 1 consisted of 11 primiparous goats and group 2 consisted of 13 multiparous goats. Estrus was synchronized by 20 mg fluorogestone acetate (FGA)-impregnated intravaginal sponges and the IM administration of 125 mg of cloprostenol (PGF2\alpha) and eCG (400 IU), 48 h before sponge removal. Intrauterine fixed time artificial insemination (AI) was carried out 55 h after removal of the FGA sponges. Results of the present study indicate …


A Loop-Mediated Isothermal Amplification (Lamp) Assay For Rapid Detection Of Anaplasma Phagocytophilum Infection In Dogs, Chungchen Lee, Yungchang Lin, Chauloong Tsang, Yangtsung Chung Jan 2012

A Loop-Mediated Isothermal Amplification (Lamp) Assay For Rapid Detection Of Anaplasma Phagocytophilum Infection In Dogs, Chungchen Lee, Yungchang Lin, Chauloong Tsang, Yangtsung Chung

Turkish Journal of Veterinary & Animal Sciences

A simple and rapid diagnostic method of Anaplasma phagocytophilum infection was established, employing the loop-mediated isothermal amplification (LAMP) reaction. With 1 set of 4 specific primers targeting the citrate synthase (gltA) gene of A. phagocytophilum, the LAMP assay rapidly amplified the target DNA sequence in a single tube in 1 h, requiring only a water bath for reaction. When visualized by gel electrophoresis, the LAMP products appeared as a ladder-like pattern, with many bands of different sizes from 180 base pairs up to the loading well. The results obtained from testing 32 clinical blood samples of dogs demonstrated that the …


Serum Protein Fractions In Brazilian-Breed Donkeys Using Agarose Gel Electrophoresis, Paulo Henrique Cavalcante, Andrea Cristina Silva, Sidnei Sakamoto, Benito Soto-Blanco Jan 2012

Serum Protein Fractions In Brazilian-Breed Donkeys Using Agarose Gel Electrophoresis, Paulo Henrique Cavalcante, Andrea Cristina Silva, Sidnei Sakamoto, Benito Soto-Blanco

Turkish Journal of Veterinary & Animal Sciences

The values for the serum protein profiles of horses are available in many studies. However, only one study has characterized the electrophoretic migration profile of plasma protein in donkeys. The present study aimed to determine by agarose gel electrophoresis the normal values for serum protein fractions in erratic Brazilian-breed donkeys and to compare these values with those of the serum proteins of horses. Serum samples were collected from 26 donkeys of the Brazilian-breed and from 10 healthy horses. The serum levels of the total proteins were determined by the biuret method. The separation and determination of the values of serum …