Medicine and Health Sciences Commons^™

Preparation, Characterization, And Delivery Of Antibodies Binding To A Model Oncogenic Rna, Human Initiator Trna, Jennifer Archer

Electronic Theses and Dissertations

Non-coding RNAs (ncRNAs) account for a higher percent of the genome than coding mRNAs, and are implicated in human disease such as cancer, neurological, cardiac and many others. While the majority of ncRNAs involved in disease were originally attributed to a class of RNAs called micro RNAs (miRNAs) with a small size of only about 19 -24 base pairs, emerging research has now demonstrated a class of long non-coding RNAs (lncRNAs) that have a size of over 200 base pairs to be responsible for gene regulation and other functional roles and have also found to contribute to pathogenesis in humans. …

The Role Of Lim Kinase 1 And Its Substrates In Cell Cycle Progression, Lisa Ritchey

Electronic Theses and Dissertations

LIM Kinase 1 (LIMK1), a modulator of actin and microtubule dynamics, has been shown to be involved in cell cycle progression. In this study we examine the role of LIMK1 in G1 phase and mitosis. We found ectopic expression of LIMK1 resulted in altered expression of p27Kip1, the G1 phase Cyclin D1/Cdk4 inhibitor. Overexpression of LIMK1 resulted in lower levels of p27Kip1 and p27Kip1-pY88 (inactive p27Kip1). Knockdown of LIMK1 resulted in elevated levels of p27Kip1 and p27Kip1-pY88. Together, these results suggest LIMK1 regulates progression of G1 phase through modulation of p27Kip1 expression. LIMK1 is involved in the mitotic process through …

Management Of Breast Cancer In The Medicaid Population, Rohan Mahabaleshwarkar

Electronic Theses and Dissertations

Breast cancer is the second most comcancer and the second leading cause of cancer-related death among women. The current project examined some key issues important for effective breast cancer management in the Medicaid population. Medicaid is one the largest healthcare insurance systems in the US providing coverage to more than 60 million low-income individuals. As a part of this project, three studies were conducted. The first study determined the healthcare burden associated with breast cancer in the form of healthcare use (inpatient, outpatient, and emergency room [ER] visits) and costs associated with the condition in the Medicaid population. Significant healthcare …

Cranberry Metabolites With Urinary Anti-Adhesion Activity, Christina M. Coleman

Electronic Theses and Dissertations

The fruit of the American cranberry (Vaccinium macrocarpon Aiton, Ericaceae) is a comfood and a top selling dietary supplement in the U.S. Cranberry juice is traditionally used for the prevention of urinary tract infections (UTIs), many of which are caused by P-fimbriated Escherichia coli. Human urine produced after cranberry juice consumption can prevent E. coli adhesion, but the urinary metabolites responsible for this activity are currently not known. High-molecular weight components of cranberry juice, specifically proanthocyanidin oligomers (PACs), are currently believed to be responsible for the ability of cranberry juice to prevent UTIs. Cranberry PACs can prevent the adherence of …

Structure, Synthesis And Biological Activity Of Harmful Algal Bloom Toxins From Karlodinium Sp. And Exploration Of Microbial Derived Natural Products, Amanda Leigh Waters

Electronic Theses and Dissertations

This work features natural products isolated from both the marine and microbial environments. Part I focuses on the elucidation of toxins from harmful algal blooms (HAB). The HAB dinoflagellate Karlodinium veneficum produces a harmful suite of molecules identified as the karlotoxins (KmTx) that have been implicated in massive fish kills pan-globally. Nine new karlotoxin congeners were isolated from various Karlodinium blooms around the world and utilizing the structure of KmTx2 were characterized using overlaid 2D NMR techniques. Due to the contrasting conformational differences between KmTx and the closely related amphidinols, the absolute configuration of KmTx was further evaluated by extensive …

Intranasal Delivery Of Therapeutics Targeted To Csf And Brain, Prashanth Manda

Electronic Theses and Dissertations

Blood-brain barrier and blood-cerebrospinal fluid barrier limit the systemic delivery of therapeutics to the brain and cerebrospinal fluid (CSF) respectively. Therapeutic agents targeting CNS are currently being administered by invasive routes which are not patient compliant and do not allow frequent administration. Intranasal delivery of therapeutics to target CSF and brain is a noninvasive route of administration which is patient compliant and allows frequent administration. In the current study, the plausibility of delivering ziconotide and cefotaxime to CSF and brain respectively via intranasal pathway using barrier modulating agents like chitosan was investigated. The ability of chitosan to enhance the permeation …

Secondary Metabolite Production By An Endophytic Bacillus Sp. From Platanus Occidentalis, Michael C. James

Electronic Theses and Dissertations

Endophytes are bacterial and fungal microbes that live inside of plant tissue without causing disease. Endophytes have applications in many industries including the pharmaceutical and food industries as producers of natural products. This potential was demonstrated by investigating the natural product production from a Bacillus amyloliquefaciens endophyte of Platanus occidentalis using a combination of MALDI-IMS, LC-MS, MS-MS, and NMR methods, by purifying nicotianamine from soy flour, and by reviewing natural product inhibitors of the hepatitis C virus (HCV). The secondary metabolite production of the endophytic B. amyloliquefaciens was investigated under a variety of fermentation conditions. It was discovered that the …

Pilot Study Of Safe Sisters: A Sexual Assault Prevention Program For Sorority Women, Alicia Pugh Cambron

Electronic Theses and Dissertations

Sexual violence is a serious problem that can have lasting, harmful effects on victims and their family, friends, and communities (CDC, 2014). Approximately one in five women will become a victim of sexual violence at some point in their lifetime (RAINN, 2009). College women are at a greater risk for rape and other forms of sexual assault than women in the general population (Fisher, Cullen & Turner, 2000). During an academic school year, approximately three percent of college women became a victim of sexual violence (Tijaden & Thoennes, 1998). Sorority women are four times more likely to experience sexual violence …

The Effects Of High-Intensity Interval Training And 28 Days Of [Beta]-Hydroxy-[Beta]-Methybutyrate Supplementation On Measures Of Aerobic Power And Metabolic Thresholds, Edward Robinson

Electronic Theses and Dissertations

Purpose: To examine the effects of 28 days of β-hydroxy-β-methylbutyrate free acid (HMB) and high-intensity interval training (HIIT) on maximal oxygen consumption (VO2peak), ventilatory threshold (VT), respiratory compensation point (RCP) and time to exhaustion (Tmax) in collegeaged men and women. Methods: Healthy men and women (n=34, age and VO2peak= 22.7+3.1yr and 39.3+5.0 mL. kg-1.min-1, respectively) participated in this study. All participants completed a series of tests prior and subsequent to treatment. A maximal oxygen consumption test was performed on a cycle ergometer to assess VO2peak, Tmax, VT, and RCP. The peak power output (Ppeak), power at VT (PVT) and power …

Mobile Phone Short Message Service (Sms) To Improve Malaria Pharmacoadherence In Zambia, Elinda Steury

Electronic Theses and Dissertations

Malaria significantly contributes to morbidity and mortality rates in Zambia. The currently accepted malaria treatment is artemisinin-based combination therapy (ACT); it is more than 97% effective when the regimen is strictly adhered to. However, the mean ACT adherence rate in sub-Saharan Africa is only approximately 38-48%. Poor pharmacoadherence remains a significant barrier to malaria control and elimination. The purpose of this study was to determine if adherence rates to a six-dose ACT antimalarial treatment differ between patients in Zambia who received short message service (SMS) reminders and those who did not. An experimental, randomized, controlled trial was conducted to collect …

Role Of Cardiac Catecholamines In Embryos And Adults Under Stress, Candice Baker

Electronic Theses and Dissertations

Cardiovascular disease is responsible for the loss of one life every 38 seconds and accounts for 26.6 percent of all infants that die of congenital birth defects. Adrenergic hormones are critically important regulators of cardiovascular physiology in embryos and adults. They are key mediators of stress responses and have profound stimulatory effects on cardiovascular function, and dysregulation of adrenergic function has been associated with many adverse cardiac conditions, including congenital malformations, arrhythmias, ischemic heart disease, heart failure, and sudden cardiac death. Despite intensive study, the specific roles these hormones play in the developing heart is not well-understood. Further, there is …

Perceptual Analysis Of Prolonged Vowels In Adolescent And Young Adults With Friedreich's Ataxia, Tara Varsallone

Electronic Theses and Dissertations

The purpose of this study was to conduct perceptual analyses, using the initial two-second portions, of prolonged vowels /a/, /i/, and /o/. Two groups of adolescents and young adults were analyzed- one group consisting of 20 individuals with Friedreich's ataxia who were compared to 20 individuals with normal voice (control participants). A trained group of 10 graduate students listened to 132 vowel samples (3 vowels X 40 participants, + 12 samples (10%) for reliability purposes) for a total of 132 perceptual judgments. The students listened to the samples which were randomized onto Dell computers (Optiplex 755) and played through headphones …

Augmentative-Alternative Communication Access For Individuals With Communication Disorders In Medical Settings, Stephanie Amundsen

Electronic Theses and Dissertations

This study surveyed speech-language pathologists (SLPs) working in medical settings in the state of Florida in order to: (a) assess the availability of AAC devices, related materials, and services in acute, sub-acute, long-term care, and outpatient medical facilities, (b) examine barriers and supports in providing AAC services to patients with complex communication needs (CCN) in the aforementioned settings, and (c) determine perceived levels of AAC knowledge of health care practitioners. One of the study's major findings was that 97.59% of SLPs served at least one patient they identified as having CCNs, and 94.1% of respondents indicated that their patients could …

Effect Of Acute L-Alanyl-L-Glutamine (Sustamine) And Electrolyte Ingestion On Cognitive Function, Multiple Object Tracking And Reaction Time Following Prolonged Exercise, Gabriel Pruna

Electronic Theses and Dissertations

Changes in physiological function occurring during a body water deficit may result in significant decrements in performance, cognitive function and fine motor control during exercise. This may be due to the magnitude of the body water deficit. Rehydration strategies are important to prevent these deleterious effects in performance. The purpose of this study was to examine the changes before and after prolonged exercise of an alanine-glutamine dipeptide (AG) on cognitive function and reaction time. Twelve male endurance-trained runners (age: 23.5 [plus or minus] 3.7 y; height: 175.5 [plus or minus] 5.4 cm; weight: 70.7 [plus or minus] 7.6 kg) participated …

Factors Associated With Information Literacy Competencies Of The Traditional Baccalaureate Nursing Student, Patricia Lafferty

Electronic Theses and Dissertations

Nursing practice at all levels requires a nurse to use evidence-based nursing to improve the quality of patient care. Registered nurses need information literacy skills to practice evidence-based nursing, therefore, all nursing students need to be information literate upon graduation from nursing programs. There is no empirical research evidence on information literacy skills for students entering nursing programs in four-year colleges. Students seeking to attain a baccalaureate degree through a traditional nursing program, enter with differing demographic and educational factors that may affect their information literacy skills. Therefore, the purpose of this research was to examine information literacy skills of …

Effect Of Acute L-Alanyl-L-Glutamine (Sustamine) And Electrolyte Ingestion On Plasma Electrolytes, Physiologic Measures, And Neuromuscular Fatigue During Endurance Exercise, William Mccormack

Electronic Theses and Dissertations

The purpose of this study was to compare the efficacy of two dose levels of L-Alanyl-LGlutamine in a commercially available sports drink to the sports drink only on time to exhaustion, neuromuscular fatigue and physiological measures during prolonged endurance exercise. Twelve endurance-trained males (23.5±3.7 yrs; 175.5±5.4 cm; 70.7±7.6 kg) performed four trials, each consisting of 1 hr treadmill runs at 75% of VO2peak followed by a run to exhaustion at 90% of VO2peak. The trials differed in type of hydration. One trial consisted of no hydration (NHY), another required ingestion of only a sports drink (ET), and two trials required …

Barriers To Membership In The American Dental Hygienists’ Association In The State Of Georgia, Brandy Henderson

Electronic Theses and Dissertations

Professional associations must have a significant level of membership to be effective. Georgia membership is increasingly low; therefore, ADHA cannot represent dental hygienists’ interests. This study determined factors that caused dental hygienists to continue to forgo membership in the ADHA. Several theoretical views of professional membership were considered. The sample was acquired from an unbiased systematic sampling of 50% (3,270) of registered dental hygienists and a convenience sampling of ADHA nonmembers at 2 continuing education seminars in Georgia. Data collection procedures included an electronic cover letter, consent form, and survey via Survey Monkey or hard copies for seminars. Three hundred …

An Evaluation Of The Quality Assurance Plan At East Tennessee State University’S Dental Hygiene Program, Jennifer E. Fielden

Electronic Theses and Dissertations

Quality assurance in healthcare is fundamental in ensuring the achievement of desired outcomes for patients. In 2011 a quality assurance plan was created at the East Tennessee State University (ETSU) dental hygiene program in order to meet accreditation standards. The purpose of this study was to evaluate this plan in order to determine its effectiveness in improving the quality of patient care indicators. One hundred fifty patient charts were selected and audited. Deficiencies were counted in the categories of assessment, treatment, documentation, referral, caries management, perio management, patient education, and follow-up. Research findings were varied; however, external variables with the …

Retention Strategies For Medical Technologists: Addressing The Shortages And Vacancies In The Clinical Laboratory, Kathy S. Small

Electronic Theses and Dissertations

It is important to have well-trained and qualified laboratory professionals. Seventy percent of patient care is based on decisions made from laboratory results, yet there is a growing shortage of medical technologists. Although some baby boomers are delaying retirement, worsening of the shortage crisis is inevitable. Retention of medical technologists has become more important than recruitment. The purpose of this study was to identify and evaluate innovative retention strategies used by clinical laboratory managers throughout the United States.

A significant finding of this study was the lack of qualified medical technologists entering the ranks of laboratory managers. This study identified …

Who Done It? Rurality Vs. Ses As Critical Factors In Evaluating The Prevalence Of Child Psychosocial Concerns In Primary Care, Robert M. Tolliver

Electronic Theses and Dissertations

The purpose of this study was to evaluate the prevalence of child psychosocial concerns in rural primary care, hypothesized to be greater than national averages due to lacking mental health services in rural areas. This study was an examination of the role of SES, various definitions of “rural,” and the interaction of SES and rurality, in predicting parent-reported child psychosocial concerns in Appalachian primary care clinics. Caregivers presenting with their child at one of 8 pediatric primary care sites (n=2,672) were recruited to complete a measure assessing demographics and the Pediatric Symptom Checklist (PSC). Results showed that while rural status …

Anxiety In Children With Williams Syndrome : Association With Negative Reactivity, Self-Regulation, And Sensory Modulation., Nicole A. Crawford-Zelli

Electronic Theses and Dissertations

Williams syndrome (WS) is a neurodevelopmental disorder caused by a submicroscopic deletion of approximately 25 genes on chromosome 7q11.23 (Hillier et al., 2003). This genotype is associated with a characteristic set of phenotypic features including mild to moderate intellectual disability, a distinctive cognitive profile, facial dysmorphology, common personality traits, cardiovascular problems, and connective tissue disorders (Morris, 2010). In addition, behavioral difficulties such as anxiety symptoms (e.g., Leyfer, Woodruff-Borden, & Mervis, 2009), negative reactivity (e.g., Davies, Udwin, & Howlin, 1998), problems with self-regulation (e.g., Woodruff-Borden, Kistler, Henderson, Crawford, & Mervis, 2010), and sensory modulation difficulties (e.g., John & Mervis, 2010) are …

Effects Of High Fidelity Simulation On Knowledge Acquisition, Self-Confidence, And Satisfaction With Baccalaureate Nursing Students Using The Solomon-Four Research Design, Rachel M. Hall

Electronic Theses and Dissertations

High Fidelity Simulation is a teaching strategy that is becoming well-entrenched in the world of nursing education and is rapidly expanding due to the challenges and demands of the health care environment. The problem addressed in this study is the conflicting research results regarding the effectiveness of HFS for students’ knowledge acquisition after participating in simulation exercises. Specifically this researcher determined the effects of a formatted simulation scenario on knowledge acquisition among nursing students and the students’ satisfaction and selfconfidence with the simulation learning activity. Kolb’s Experiential Learning Theory (1984) provided the framework for this study.

This study used a …

Health Service Utilization And Stigma Among Hiv-Positive Men-Who-Have-Sex-With Men (Msm) In Rural Appalachia, Roger L. Blackwell Jr

Electronic Theses and Dissertations

ABSTRACT

Health Service Utilization and Stigma among

HIV-Positive Men-Who-Have-Sex-With-Men (MSM) in Rural Appalachia

by

Roger Lee Blackwell

The world has now entered the third decade of the AIDS epidemic. Men-who-have-sex-with-men continue to be disproportionately affected by HIV/AIDS. The United States still struggles in its response to this ongoing crisis in many areas: disease prevention, treatment, and HIV related stigma, prejudice and discrimination. Much of the information reported on MSM living with HIV has come from urban population centers, but only a few studies have focused on HIV positive MSM living in rural areas. Therefore, the overall aim of this dissertation …

Becoming The Best Mom Or The Best Doctor? Gender Inequality And Medical Students’ Specialty Choice, Casey L. Lawson

Electronic Theses and Dissertations

In anticipation of an expected national shortage of primary care physicians, 24 medical students from the East Tennessee State University Quillen College of Medicine were selected through a snowball sample and participated in in-depth interviews. A major aim of the study was to explore the social and economic factors influencing students’ specialty choice and career interests. Students’ perceptions of “rural” environments, student debt, professional obligations, specialties, and preceptorship experiences were analyzed. Students’ gender heavily influenced their feelings about choosing a medical specialty, as did their stereotypes of physicians in particular medical fields. The thesis concludes with recommendations for challenging negative …

Cyclin E Induction And Oncolytic Replication Of E1b-Deleted Adenoviruses., Pei-Hsin Cheng

Electronic Theses and Dissertations

Virus-mediated oncolysis has been considered as a new and promising cancer therapeutic approach. Although adenoviruses (Ads) with deletion of E1b55K preferentially replicate in cancer cells and have been used in numerous cancer treatments, the selective replication mechanism of this kind of virus still remains controversial. The lack of a well-established studies focusing on possible mechanisms enabling tumor selectivity of oncolytic Ads has hindered the further development of virotherapies and limits their clinical applications. Therefore, uncovering the molecular basis behind the tumor-killing phenomena will fill critical gaps in our understanding of the oncolytic adenovirology. Previously our laboratory has demonstrated that Ad …

Exercise Participation During Weight Loss On A High Protein – Low Carbohydrate Diet Plan In Females Aged 15-25 Years, Margaret Mobley-Meulman

Electronic Theses and Dissertations

Weight gain due to poor diet and lack of exercise is responsible for over 300,000 deaths each year (U.S. Department of Health and Human Services, 2010). Obese adults have an increased risk for serious health conditions including high blood pressure and cholesterol, type 2 diabetes, coronary heart disease, stroke, gallbladder disease, osteoarthritis, sleep apnea, respiratory problems, and certain cancers (National Cancer Institute, 2012). Participation in exercise can help control weight, strengthen muscles and bones, and reduce the incidence of cardiac events, stroke, hypertension, type 2 diabetes, colon and breast cancers, osteoporotic fractures, gallbladder disease, obesity, depression, anxiety, and delay mortality …

Screening The Safety Net, Babette L. Southard Mrs

Electronic Theses and Dissertations

Safety net clinics across the country struggle with a lack of resources to tackle the needs presented. Screening programs set up for children and elderly have proven to be effective in triaging need, prioritizing care, and maximizing resources. These programs do not currently exist for working uninsured adults. Research was initiated to answer the question: Does the screening process improve patient care for the community clinic? During a 6-week pilot study a licensed dental hygienist performed 30 screenings in the community clinic setting. Findings were recorded and coded according to patient’s level of need identified. Pre- and posttest data for …

Unpacking The Temperament Weight Relationship: The Mediating Role Of Food Preferences, Sarah A. Berry

Electronic Theses and Dissertations

The current study examined the mediating role of possible food preferences on the temperamentweight relationship among 18-month-old toddlers. Parents of 37 typically developing toddlers completed the Early Childhood Behavior Questionnaire (ECBQ). During a lab visit toddlers’ weight and recumbent length were measured and recorded. Toddlers also completed a sequential touching task to examine their ability to categorize a healthy group of foods and an unhealthy group of foods. The only temperament measure found to associate with both child weight status and food categorization was inhibitory control. Toddlers’ food categorization was not found to mediate the relationship between inhibitory control and …

Efficacy Of Partial Rom Squat In Maximal Strength Training, Caleb Bazyler

Electronic Theses and Dissertations

Eighteen well trained males (1RM Squat: 150.57 ± 26.79 kg) were assigned to two groups: full ROM training (control) and full ROM with partial ROM training (CP) for the seven-week training intervention. There was a significant time effect (p

The Relationship Of Stress Levels To Wellness Practices Among U.S. Radiologic Technology Program Directors, Jesse D. Pennington

Electronic Theses and Dissertations

The purpose of this modified replication study is to examine the relationship between the stress levels and the wellness practices of U.S. radiologic technology program directors and to determine if correlations exist between wellness practices and stress levels of the group. Additionally, relaxation activities and the feelings, characteristics, and symptoms associated with stress were considered. The design of the study was ex post facto correlational. The population of the study was U.S. radiologic technology program directors working in JRCERT accredited programs. The electronic instrument was distributed to 635 program directors through e-mail, with 424 (66.7%) usable returns. Significant associations were …