Medicine and Health Sciences Commons^™

Population Genomics Of Invasive Rodents On Islands: Genetic Consequences Of Colonization And Prospects For Localized Synthetic Gene Drive, Kevin P. Oh, Aaron B. Shiels, Laura Shiels, Dimitri V. Blondel, Karl J. Campbell, J. Royden Saah, Alun L. Lloyd, Paul Q. Thomas, Fred Gould, Zaid Abdo, John R. Godwin, Antoinette J. Piaggio

United States Department of Agriculture Wildlife Services: Staff Publications

Introduced rodent populations pose significant threats worldwide, with particularly severe impacts on islands. Advancements in genome editing have motivated interest in synthetic gene drives that could potentially provide efficient and localized suppression of invasive rodent populations. Application of such technologies will require rigorous population genomic surveys to evaluate population connectivity, taxonomic identification, and to inform design of gene drive localization mechanisms. One proposed approach leverages the predicted shifts in genetic variation that accompany island colonization, wherein founder effects, genetic drift, and island-specific selection are expected to result in locally fixed alleles (LFA) that are variable in neighboring nontarget populations. Engineering …

Thyreos Completes Seed Funding From Invest Nebraska, Strategic Angel Investors, Nebraska Combine

The Combine

First paragraph:

Thyreos (https://www.thyreosvaccines.com/), a vaccine company that is developing a novel vaccine platform that protects against a range of herpesviruses, has announced a $750,000 investment round led by Invest Nebraska with participation from other local angel investors in the animal health and veterinary space.

Identification Of A Chlorovirus Pbcv-1 Protein Involved In Degrading The Host Cell Wall During Virus Infection, Irina V. Agarkova, Leslie C. Lane, David D. Dunigan, Cristian F. Quispe, Garry A. Duncan, Elad Milrot, Abraham Minsky, Ahmed Esmael, Jayadri S. Ghosh, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Chloroviruses are unusual among viruses infecting eukaryotic organisms in that they must, like bacteriophages, penetrate a rigid cell wall to initiate infection. Chlorovirus PBCV-1 infects its host, Chlorella variabilis NC64A by specifically binding to and degrading the cell wall of the host at the point of contact by a virus-packaged enzyme(s). However, PBCV-1 does not use any of the five previously characterized virus-encoded polysaccharide degrading enzymes to digest the Chlorella host cell wall during virus entry because none of the enzymes are packaged in the virion. A search for another PBCV-1-encoded and virion-associated protein identified protein A561L. The fourth domain …

Categorization Of Orthologous Gene Clusters In 92 Ascomycota Genomes Reveals Functions Important For Phytopathogenicity, Daniel Peterson, Tang Li, Ana M. Calvo, Yanbin Yin

Food for Health: Publications

Phytopathogenic Ascomycota are responsible for substantial economic losses each year, destroying valuable crops. The present study aims to provide new insights into phytopathogenicity in Ascomycota from a comparative genomic perspective. This has been achieved by categorizing orthologous gene groups (orthogroups) from 68 phytopathogenic and 24 non-phytopathogenic Ascomycota genomes into three classes: Core, (pathogen or non-pathogen) group-specific, and genome-specific accessory orthogroups. We found that (i) ~20% orthogroups are group-specific and accessory in the 92 Ascomycota genomes, (ii) phytopathogenicity is not phylogenetically determined, (iii) group-specific orthogroups have more enriched functional terms than accessory orthogroups and this trend is particularly evident in phytopathogenic …

A High-Androgen Microenvironment Inhibits Granulosa Cell Proliferation And Alters Cell Identity, Renee M. Mcfee, Sarah M. Romereim, Alexandria P. Snider, Adam F. Summers, William E. Pohlmeier, Scott G. Kurz, Robert A. Cushman, John S. Davis, Jennifer R. Wood, Andrea S. Cupp

School of Veterinary and Biomedical Sciences: Faculty Publications

A naturally occurring bovine model with excess follicular fluid androstenedione (High A4), reduced fertility, and polycystic ovary syndrome (PCOS)-like characteristics has been identified. We hypothesized High A4 granulosa cells (GCs) would exhibit altered cell proliferation and/or steroidogenesis. Microarrays of Control and High A4 GCs combined with Ingenuity Pathway Analysis indicated that High A4 GCs had cell cycle inhibition and increased expression of microRNAs that inhibit cell cycle genes. Granulosa cell culture confirmed that A4 treatment decreased GC proliferation, increased anti-Müllerian hormone, and increased mRNA for CTNNBIP1. Increased CTNNBIP1 prevents CTNNB1 from interacting with members of the WNT signaling pathway thereby …

Non-Nutritive Suck Burst Pattern Stability In Extremely Premature Infants, Alejandra Marquez

College of Education and Human Sciences: Dissertations, Theses, and Student Research

The development of non-nutritive suck (NNS) burst dynamics in preterm infants reflects the integrity of the brain and is used clinically to assess feeding readiness and orofacial motor development (Mizuno and Ueda, 2005). The application of NNS analytics in the present report represents one outcome measurement set that is part of an ongoing clinical trial involving extremely preterm infants (EPI’s,[GA]) randomized to receive either pulsed orocutaneous stimulation therapeutics or a sham (blind pacifier), in conjunction with salivary sampling twice weekly to map gene expression of key proteins involved in neural development and molecular sensing of feeding related pathways in the …

Clinical Case Study: The Effects Of Real-Time Iemg Biofeedback On Facial Muscle Activation Patterns In A Child With Congenital Facial Palsy, Alyssa Molina

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Experimental findings are limited concerning efficacious procedures for facial reanimation following persistent idiopathic facial paralysis or paresis. Additional research examining the use of real-time integrated electromyography (iEMG) biofeedback for young children with persistent idiopathic facial paralysis is one approach to further understand the efficacy of electrophysiology biofeedback techniques in facial reanimation for this age group and etiology. iEMG research has applications in interventions for pathology of nerves and muscles affecting animation of the face. The proposed clinical case study will adopt real time iEMG visual biofeedback to facilitate facial reanimation in a preadolescent child. Quantitative measurements will determine orofacial muscle …

An Analysis Of The Behaviors Used By Physicians And (Pre)Medical Students To Cope With Stress, Carter Bracht

Honors Program: Senior Projects (Public)

Physicians (both practicing and resident) in the U.S. are experiencing high rates of burnout and mental health issues because of the stressors related to their career and the lack of coping mechanisms in practice by these physicians. As a population, physicians normalize internalization of their stress as members of a “proud” community. This study sought to examine the positive, negative, and positive addiction coping mechanisms that physicians use to relieve job-related stress using the following coping domains: emotion-oriented, task-oriented, and avoidance-oriented coping. We further sought to examine stressors and coping mechanisms that are introduced in (pre)medical school and how they …

Using Enclosed Y-Mazes To Assess Chemosensory Behavior In Reptiles, M. Rockwell Parker, Andrea F. Currylow, Eric A. Tillman, Charlotte J. Robinson, Jilian M. Josimovich, Isabella M.G. Bukovich, Lauren A. Nazarian, Melia G. Nafus, Bryan M. Kluever, Amy A. Yackel Adams

United States Department of Agriculture Wildlife Services: Staff Publications

Reptiles utilize a variety of environmental cues to inform and drive animal behavior such as chemical scent trails produced by food or conspecifics. Decrypting the scent-trailing behavior of vertebrates, particularly invasive species, enables the discovery of cues that induce exploratory behavior and can aid in the development of valuable basic and applied biological tools. However, pinpointing behaviors dominantly driven by chemical cues versus other competing environmental cues can be challenging. Y-mazes are common tools used in animal behavior research that allow quantification of vertebrate chemosensory behavior across a range of taxa. By reducing external stimuli, Y-mazes remove confounding factors and …

Lessons Learned: New Insights On The Role Of Cytokines In Covid-19, Maja Buszko, Aleksandra Nita-Lazar, Jung Hyun Park, Pamela L. Schwartzberg, Daniela Verthelyi, Howard A. Young, Amy S. Rosenberg

United States Public Health Resources

In the midst of resurging COVID-19 cases, the second NIH/FDA virtual COVID-19 and Cytokines symposium was held on 1 December 2020, focusing on longitudinal studies of COVID-19 immunity, including long-term consequences, potential associations with autoimmunity and the multisystem inflammatory syndrome in children (MIS-C).

A central and ongoing quest in COVID-19 research is to establish why and how SARS-CoV-2 elicits heterogeneity in disease severity and immunopathology among infected individuals. Hence, much effort has been exerted to understand the cellular basis of SARS-CoV-2-induced immune responses, with the aim of identifying new biomarkers and prognostic tools and developing new therapeutic options. Cytokines emerged …

Program And Proceedings: Nebraska Academy Of Sciences 1880–2021, 141st Anniversary Year, One Hundred-Thirty-First Annual Meeting, April 23-24, 2021

Nebraska Academy of Sciences: Programs and Proceedings

Program

Aeronautics and Space Science

Anthropology

Applied Science and Technology

Biological and Medical Sciences

Collegiate Academy: Biology

Collegiate Academy: Chemistry and Physics

Earth Sciences

Environmental Sciences

General Chemistry

General Physics

Teaching of Science and Mathematics

Friend of Science Award: Paul Karr

Rejection Of African Indigenous Food: The Case Of Rwanda, Eugene Baraka

NUTR/GLST 498b: Global Research Experiences in Nutrition and Health

Rwanda has one of the highest stunting rates among children globally, and malnutrition remains one of the most pressing public health issues. Lack of diversified diets, due to colonialization and nutrition transition, is the leading cause of the double burden of malnutrition in the country where the progress to reduce undernutrition is slow and obesity rates are rapidly rising. Consumption of indigenous foods has proven to diversify diets, but very few studies have been conducted to assess which ones are available for consumption in Rwanda. The purpose of this study was to assess the availability and use of indigenous foods …

Unprecedented Migratory Bird Die-Off: A Citizen-Based Analysis On The Spatiotemporal Patterns Of Mass Mortality Events In The Western United States, Di Yang, Anni Yang, Jue Yang, Rongting Xu, Han Qiu

United States Department of Agriculture Wildlife Services: Staff Publications

Extensive, severe wildfires, and wildfire-induced smoke occurred across the western and central United States since August 2020. Wildfires resulting in the loss of habitats and emission of particulate matter and volatile organic compounds pose serious threatens to wildlife and human populations, especially for avian species, the respiratory system of which are sensitive to air pollutions. At the same time, the extreme weather (e.g., snowstorms) in late summer may also impact bird migration by cutting off their food supply and promoting their migration before they were physiologically ready. In this study, we investigated the environmental drivers of massive bird die-offs by …

The Importance Of Nutrition And Preventing Malnutrition In Older Adults: A Literature Review And Informational Booklet, Sophie Johnson

Honors Program: Senior Projects (Public)

Over years of studying nutrition science and gerontology, there was very little information in classes that combined these two important topics. Older adults are often classified as those above the age of 65 years old. There is a growing number of older adults in the United States, which increases the need for information on how nutrition and aging are interlaced. As humans age, multiple factors can impact nutrition. Nutrition involves consuming the proper amount of nutrients to maintain body function. There are physiological, physical, socioeconomic and medication factors that can all influence nutrition status. It is important to be aware …

Discussion Of “Is Group Testing Ready For Prime-Time In Disease Identification?” By Haber, Malinovsky, And Albert, Statistics In Medicine, 2021, Brad J. Biggerstaff

United States Public Health Resources

I congratulate the authors on an interesting, instructive, and timely paper. Certainly, including the testing for SARS-CoV-2 example in a paper on a topic—group testing—of wide interest during the current COVID-19 pandemic emphasizes the relevance of the topic in public health and diagnostic medicine generally. But the thoughtful inclusion of the remaining examples—HIV, HPV, and cancer biomarker detection—illustrates that the issues considered are important even in less urgent times. In this note of discussion I will comment on two particular aspects in the paper that I found particularly informative, and then I will comment on two other, related areas that …

Spoken Barriers: The Effects Of Spanish-English Interpretation Within The United States Health System, Michelle Lounsbery

Honors Program: Senior Projects (Public)

In recent times the prevalence of the Spanish language in the United States has been on a steady incline, and the language barrier created between patients and providers is only growing. Studies display a direct relationship between communication barriers formed by insufficient interpretation methods and negative patient outcomes due to a lack of understanding. This study will evaluate of the effects of Spanish-English interpretation in the United States health system as well as review the significance of direct patient-provider communication and how to improve these methods. Research methods will involve the use of other accredited academic literature and studies, as …

Maldi-Tof Ms Biomarker Detection Models To Distinguish Rtx Toxin Phenotypes Of Moraxella Bovoculi Strains Are Enhanced Using Calcium Chloride Supplemented Agar, Matthew M. Hille, Michael L. Clawson, Aaron M. Dickey, Justin H. Lowery, John Dustin Loy

School of Veterinary and Biomedical Sciences: Faculty Publications

Moraxella bovoculi is the bacterium most often cultured from ocular lesions of cattle with infectious bovine keratoconjunctivitis, also known as bovine pinkeye. Some strains of M. bovoculi contain operons encoding for a repeats-in-toxin (RTX) toxin, which is a known virulence factor of multiple veterinary pathogens. We explored the utility of MALDI-TOF MS and biomarker detection models to classify the presence or absence of an RTX phenotype in M. bovoculi. Ninety strains that had undergone whole genome sequencing were classified by the presence or absence of complete RTX operons and confirmed with a visual assessment of hemolysis on blood agar. Strains …

Food Sourcing And The Relationship To Height Of 6th To 7th Graders In Southern Ethiopia And Zambia, Fulxen Ignacio Bescansa Luers

NUTR/GLST 498b: Global Research Experiences in Nutrition and Health

Introduction: Zambia and Ethiopia are Sub-Saharan African countries experiencing high rates of malnutrition and stunting. This study examines food sourcing and its relationship to stunting.

Methods: Anthropometric assessment and food frequency-sourcing interviews were taken for 488 6th-7th graders in Livingstone, Zambia and Hawassa, Ethiopia. Data were analyzed using ANOVA.

Results: Most students sourced food from local markets and home gardens; however Zambian students used a greater variety of sources. Those sourcing from home gardens were significantly taller.

Discussion: Home gardening provides economic and health benefits, as well as food security. A focus on preservation and use …

Rehabilitation For Anterior Cruciate Ligament Tears: A Written And Video Guide For Athletes Today And In The Future, Braeland Jones

Honors Program: Senior Projects (Public)

The following paper is a partner to a corresponding video exercise guide. The purpose of this paper is to support the exercises included in the guide with research. The research paper aids in defining which exercises are included in the first place as well as in which section they appear. The research is the literary match for the recorded exercise guide, which will be the visual side of the project. The two ideally should be read and watched together to fully understand content of both portions. The paper guide is split into three sections, evaluating the types of exercises and …

The Chromosome-Level Genome Of Dragon Fruit Reveals Whole-Genome Duplication And Chromosomal Co-Localization Of Betacyanin Biosynthetic Genes, Jinfang Zheng, Lyndel W. Meinhardt, Ricardo Goenaga, Dapeng Zhang, Yanbin Yin

Food for Health: Publications

Dragon fruits are tropical fruits economically important for agricultural industries. As members of the family of Cactaceae, they have evolved to adapt to the arid environment. Here we report the draft genome of Hylocereus undatus, commercially known as the white-fleshed dragon fruit. The chromosomal level genome assembly contains 11 longest scaffolds corresponding to the 11 chromosomes of H. undatus. Genome annotation of H. undatus found ~29,000 protein-coding genes, similar to Carnegiea gigantea (saguaro). Whole-genome duplication (WGD) analysis revealed a WGD event in the last common ancestor of Cactaceae followed by extensive genome rearrangements. The divergence …

Isometric Exercises And Stretching Warm-Up Program For University String Musicians: An Intervention Study, Emma Porter

Honors Program: Senior Projects (Public)

In this study, the purpose of this investigation was to find the effects of reducing pain through combining isometric and stretching warm-up exercises for stringed instrument musicians. In Lee’s et al. (2013) study, it showed that about 75 % of musicians have work-related musculoskeletal disorders and experience pain because of playing. In this study, they also saw that string players have the highest prevalence of both musculoskeletal disorders and pain while playing (Lee et al., 2013). In Nawrocka’s et al. (2014) journal article, the study showed the correlation of more pain in musicians that did not meet the recommended criteria …

A Kinesiological Analysis Of Dancers At The University Of Nebraska-Lincoln, Vanessa Reiser

Honors Program: Senior Projects (Public)

Supplemental conditioning can improve a dancer’s performance of important skills without taking away from class and rehearsal time. To investigate this further, an individualized conditioning plan based on a specific skill was created for eight student dancers, and the effects were analyzed using a survey and rating scale by a trained dance professor. The results showed improvement of skills for the majority of dancers, along with improved fitness in qualities such as strength and balance. In conclusion, individualized conditioning plans based on a specific skill may benefit student dancers who are juggling intense class and rehearsal schedules.

Presence Of Antibody-Dependent Cellular Cytotoxicity (Adcc) Against Sars-Cov-2 In Covid-19 Plasma, For Yue Tso, Salum J. Lidenge, Lisa K. Poppe, Phoebe B. Pena, Sara R. Privatt, Sydney J. Bennett, John R. Ngowi, Julius Mwaiselage, Michael Belshan, Jacob A. Siedlik, Morgan A. Raine, Juan B. Ochoa, Julia Garcia-Diaz, Bobby Nossaman, Lyndsey Buckner, W Mark Roberts, Matthew J. Dean, Augusto C. Ochoa, John T. West, Charles Wood

Nebraska Center for Virology: Faculty Publications

Background Neutralizing-antibody (nAb) is the major focus of most ongoing COVID-19 vaccine trials. However, nAb response against SARS-CoV-2, when present, decays rapidly. Given the myriad roles of antibodies in immune responses, it is possible that antibodies could also mediate protection against SARS-CoV-2 via effector mechanisms such as antibody-dependent cellular cytotoxicity (ADCC), which we sought to explore here. Methods Plasma of 3 uninfected controls and 20 subjects exposed to, or recovering from, SARS CoV-2 infection were collected from U.S. and sub-Saharan Africa. Immunofluorescence assay was used to detect the presence of SARS-CoV-2 specific IgG antibodies in the plasma samples. SARS-CoV-2 specific …

Deep-Learning-Based Multivariate Pattern Analysis (Dmvpa): A Tutorial And A Toolbox, Karl M. Kuntzelman, Jacob M. Williams, Phui Cheng Lim, Ashtok Samal, Prahalada K. Rao, Matthew R. Johnson

Center for Brain, Biology, and Behavior: Faculty Publications

In recent years, multivariate pattern analysis (MVPA) has been hugely beneficial for cognitive neuroscience by making new experiment designs possible and by increasing the inferential power of functional magnetic resonance imaging (fMRI), electroencephalography (EEG), and other neuroimaging methodologies. In a similar time frame, “deep learning” (a term for the use of artificial neural networks with convolutional, recurrent, or similarly sophisticated architectures) has produced a parallel revolution in the field of machine learning and has been employed across a wide variety of applications. Traditional MVPA also uses a form of machine learning, but most commonly with much simpler techniques based on …

Sars-Cov-2 Exposure In Escaped Mink, Utah, Usa, Susan A. Shriner, Jeremy W. Ellis, J. Jeffrey Root, Annette Roug, Scott R. Stopak, Gerald W. Wiscomb, Jared R. Zierenberg, Hon S. Ip, Mia Kim Torchetti, Thomas J. Deliberto

United States Department of Agriculture Wildlife Services: Staff Publications

In August 2020, outbreaks of coronavirus disease were confirmed on mink farms in Utah, USA. We surveyed mammals captured on and around farms for evidence of infection or exposure. Free-ranging mink, presumed domestic escapees, exhibited high antibody titers, suggesting a potential severe acute respiratory syndrome coronavirus 2 transmission pathway to native wildlife.

We report a wildlife epidemiologic investigation of mammals captured on or near properties in Utah, USA, where outbreaks of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection occurred in farmed mink. Mink farms are relatively common in the United States, and most are small family farms. The US …

Toxicity Of Sodium Nitrite-Based Vertebrate Pesticides For European Starlings (Sturnus Vulgaris), Scott J. Werner, Shelagh T. Deliberto, Hailey E. Mclean, Katherine E. Horak, Kirt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

In the 21st century, invasive animals rank second only to habitat destruction as the greatest threat to global biodiversity. Socially-acceptable and cost-effective strategies are needed to reduce the negative economic and environmental impacts of invasive animals. We investigated the potential for sodium nitrite (SN; CAS 7632-00-0) to serve as an avian toxicant for European starlings (Sturnus vulgaris L.). We also assessed the non-target hazard of an experimental formulation of SN that is being developed as a toxicant for invasive wild pigs (Sus scrofa L.). In gavage experiments with European starlings, we identified a lowest observed adverse effect level …

Avian Visual Ecologists Should Consider Uv Absorbance And All Sensory Modalities: A Response To Wisenden Et Al. (2020), Sean T. O'Daniels, Scott J. Werner, Ken Yasukawa

United States Department of Agriculture Wildlife Services: Staff Publications

In a recent publication, Wisenden et al. (2020) examined responses of territorial male Red-winged Blackbirds (Agelaius phoeniceus) to models constructed with ultraviolet (UV)-reflective red epaulets for the purpose of determining if the addition of UV reflectance to epaulets ("UV+") changed the effectiveness of signals to receivers relative to "control" epaulets under field conditions. The authors hypothesized that "UV+epaulet coloration represents a visual signal with increased efficacy in territorial interactions." They presented behavioral data but no visual modeling data. Our aims in this commentary are to suggest alternative terms to those used by the authors, to express concern about the use …

Exploring Attentional And Emotional Biases As A Function Of Trauma And Dissociation Symptomology, Claudia Clinchard

Department of Psychology: Dissertations, Theses, and Student Research

There is evidence that threatening facial expressions (e.g., angry faces) direct attention toward the target, and that for facial expressions that are less threatening but still convey negative valence (e.g., fear faces) direct attention outward and to one’s environment, therefore causing a shift in memory performance and attentional bias depending on the level of threat in emotional facial expressions presented. Extant literature provides evidence for attentional biases both towards and away from threat in those with posttraumatic stress disorder (PTSD) symptomology. The current study aimed to replicate the previous findings of the effects stimulus and emotion have on memory performance …

Inhibition Of Zika Virus Replication By G-Quadruplex-Binding Ligands, Indian Institute Of Technology Indore, Aryamav Pattnaik, Bikash R. Sahoo, Uma Shankar, Asit K. Pattnaik, Amit Kumar, Debasis Nayak

Nebraska Center for Virology: Faculty Publications

Zika virus (ZIKV), a mosquito-transmitted Flavivirus, emerged in the last decade causing serious diseases and affecting human health globally. Currently, no licensed vaccines or antivirals are available to combat ZIKV, although several vaccine candidates are in the pipeline. In recent years, the presence of non-canonical G-quadruplex (GQ) secondary structures in viral genomes has ignited significant attention as potential targets for antiviral strategy. In this study, we identified several novel conserved potential GQ structures by analyzing published ZIKV genome sequences using an in-house algorithm. Biophysical and biochemical analysis of the RNA sequences containing these potential GQ sequences suggested the existence of …

3d Printing Of Human Microbiome Constituents To Understand Spatial Relationships And Shape Parameters In Bacteriology, Jacques Izard, Teklu Kuru Gerbaba, Shara R. P. Yumul

Department of Food Science and Technology: Faculty Publications

Effective laboratory and classroom demonstration of microbiome size and shape, diversity, and ecological relationships is hampered by a lack of high-resolution, easy-to-use, readily accessible physical or digital models for use in teaching. Three-dimensional (3D) representations are, overall, more effective in communicating visuospatial information, allowing for a better understanding of concepts not directly observable with the unaided eye. Published morphology descriptions and microscopy images were used as the basis for designing 3D digital models, scaled at 20,000×, using computer-aided design software (CAD) and generating printed models of bacteria on mass-market 3D printers. Sixteen models are presented, including rod-shaped, spiral, flask-like, vibroid, …