Medicine and Health Sciences Commons^™

Range-Wide Declines Of Northern Spotted Owl Populations In The Pacific Northwest: A Meta-Analysis, Alan B. Franklin, Katie M. Dugger, Damon B. Lesmeister, Raymond J. Davis, J. David Wiens, Gary C. White, James D. Nichols, James E. Hines, Charles B. Yackulic, Carl J. Schwarz, Steven H. Ackers, L. Steven Andrews, Larissa L. Bailey, Robin Bown, Jesse Burgher, Kenneth P. Burnham, Peter C. Carlson, Tara Chestnut, Mary M. Conner, Krista E. Dilione, Eric D. Forsman, Elizabeth M. Glenn, Scott A. Gremel, Keith A. Hamm, Dale R. Herter, J. Mark Higley, Rob B. Horn, Julianna M. Jenkins, William L. Kendall, David W. Lamphear, Christopher Mccafferty, Trent L. Mcdonald, Janice A. Reid, Jeremy T. Rockweit, David C. Simon, Stan G. Sovern, James K. Swingle, Heather Wise

United States Department of Agriculture Wildlife Services: Staff Publications

The northern spotted owl (Strix occidentalis caurina) inhabits older coniferous forests in the Pacific Northwest and has been at the center of forest management issues in this region. The immediate threats to this federally listed species include habitat loss and competition with barred owls (Strix varia), which invaded from eastern North America. We conducted a prospective meta-analysis to assess population trends and factors affecting those trends in northern spotted owls using 26 years of survey and capture-recapture data from 11 study areas across the owls' geographic range to analyze demographic traits, rates of population change, and occupancy parameters for spotted …

Development Of Vaccines And Antivirals Against Zika Virus, Aryamav Pattnaik

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

The re-emergence of Zika virus (ZIKV), an arbovirus, poses a major global human health concern because of its ability to cause congenital abnormalities and neurological diseases. While many candidate vaccines and antiviral drugs are in the developmental pipeline, none have been approved for use against ZIKV infection. This dissertation describes the characterization of one vaccine and two antiviral drug candidates against ZIKV infection. A bacterial ferritin-based nanoparticle vaccine, termed zDIII-F, is designed to display multiple copies of ZIKV E protein domain III on its surface. These stable nanoparticles are shown to induce robust antibody-mediated protection against lethal ZIKV infection in …

Determination Of Freedom-From-Rabies For Small Indian Mongoose Populations In The United States Virgin Islands, 2019–2020, A. Springer Browne, Hannah M. Cranford, Clint N. Morgan, James A. Ellison, Are Berentsen, Nicholas Wiese, Alexandra Medley, John Rossow, Leanne Jankelunas, Alan S. Mckinley, Claudia D. Lombard, Nicole F. Angeli, Thomas Kelley, Jennifer Valiulus, Bethany Bradford, Valicia J. Burke-France, Cosme J. Harrison, Irene Guendel, Marissa Taylor, Gerard L. Blanchard, Jeffrey B. Doty, David J. Worthington, David Horner, Keith R. Garcia, Joseph Roth, Brett R. Ellis, Kristine M. Bisgard, Ryan Wallace, Esther M. Ellis

United States Department of Agriculture Wildlife Services: Staff Publications

Mongooses, a nonnative species, are a known reservoir of rabies virus in the Caribbean region. A cross-sectional study of mongooses at 41 field sites on the US Virgin Islands of St. Croix, St. John, and St. Thomas captured 312 mongooses (32% capture rate). We determined the absence of rabies virus by antigen testing and rabies virus exposure by antibody testing in mongoose populations on all three islands. USVI is the first Caribbean state to determine freedom-from-rabies for its mongoose populations with a scientifically-led robust cross-sectional study. Ongoing surveillance activities will determine if other domestic and wildlife populations in USVI are …

Testing A Bite-Shortened Hook To Minimize Fish Handling In A Recreational Fishery, Holden E. Harris, Brian K. Whalen, Andrew G. Gude, Michael S. Allen

United States Department of Agriculture Wildlife Services: Staff Publications

Discard mortality can make fishing unsustainable, even in catch-and-release or highly regulated fisheries. However, fishing practices and gears that minimize hook injury, handling, and air exposure can considerably improve fitness and survival in released fish. This study tested whether modified hooks could allow anglers to successfully land and then release fish in the water and without handling. Standard, barbless, and bite-shortened hooks were used to catch Spotted Seatrout Cynoscion nebulosus (n > 75 per hook type), and logistic models were used to assess the differences in landing success and hook self-release success by hook type and categorical fish size. Average landing …

Landscape Of Stress: Tree Mortality Influences Physiological Stress And Survival In A Native Mesocarnivore, Jennifer R. Kordosky, Eric M. Gese, Craig M. Thompson, Patricia A. Terletzky, Lorin A. Neuman-Lee, Jon D. Schneiderman, Kathryn L. Purcell, Susannah S. French

United States Department of Agriculture Wildlife Services: Staff Publications

Climate change and anthropogenic modifications to the landscape can have both positive and negative effects on an animal. Linking landscape change to physiological stress and fitness of an animal is a fundamental tenet to be examined in applied ecology. Cortisol is a glucocorticoid hormone that can be used to indicate an animal’s physiological stress response. In the Sierra Nevada Mountains of California, fishers (Pekania pennanti) are a threatened mesocarnivore that have been subjected to rapid landscape changes due to anthropogenic modifications and tree mortality related to a 4-year drought. We measured cortisol concentrations in the hair of 64 …

Risks Of Introduction And Economic Consequences Associated With African Swine Fever, Classical Swine Fever And Foot-And-Mouth Disease: A Review Of The Literature, Vienna Brown, Ryan S. Miller, Sophie C. Mckee, Karina H. Ernst, Nicole M. Didero, Rachel M. Maison, Meredith J. Grady, Stephanie A. Shwiff

United States Department of Agriculture Wildlife Services: Staff Publications

African swine fever (ASF), classical swine fever (CSF) and foot-and-mouth disease (FMD) are considered to be three of the most detrimental animal diseases and are currently foreign to the U.S. Emerging and re-emerging pathogens can have tremendous impacts in terms of livestock morbidity and mortality events, production losses, forced trade restrictions, and costs associated with treatment and control. The United States is the world's top producer of beef for domestic and export use and the world's third-largest producer and consumer of pork and pork products; it has also recently been either the world's largest or second largest exporter of pork …

Economic Losses Of Catfish To Avian Predation: A Case Report, Ganesh Kumar, Shraddha Hegde, David Wise, Charles Mischke, Brian Dorr

United States Department of Agriculture Wildlife Services: Staff Publications

Avian predation in catfish aquaculture has been a persistent issue throughout the history of the industry, and as production has expanded predation from piscivorous birds has intensified. Catfish ponds in the Mississippi River delta (in the Mississippi migratory flyway) provide a constant and readily accessible supply of forage for birds. Intensive foraging by fish-eating birds has led to a specific regulatory policy and numerous on-farm and regional management efforts. However, in 2016, legal challenges lead to recision of some federal policies and uncertainty as to allowable management, resulting in limitations on bird depredation. Estimating the extent of fish losses to …

The Value Of Republishing Scientific Literature In Institutional Repositories, Sue Ann Gardner, Linnea Fredrickson, Paul Royster

University of Nebraska-Lincoln Libraries: Presentations

Background: The University of Nebraska-Lincoln University Libraries institutional repository was founded in 2005 on the Digital Commons platform. It currently contains more than 118,000 full-text scholarly works that have been downloaded over 73,000,000 times.

Problem that drove the project: Older scientific literature may only occasionally circulate from university library collections or be purchased by readers from remaindered publishers' stock.

Methods: This research will employ statistical analyses to demonstrate the value of making available four series of scientific literature in the University of Nebraska-Lincoln University Libraries Digital Commons: Insecta Mundi, the Nebraska Bird Review, the Transactions of the Nebraska …

Attenuated Strain Of Cvb3 With A Mutation In The Car‑Interacting Region Protects Against Both Myocarditis And Pancreatitis, Ninaad Lasrado, Arunakumar Gangaplara, Chandirasegaran Massilamany, Rajkumar Arumugam, Allison Shelbourn, Mahima T. Rasquinha, Rakesh H. Basavalingappa, Gustavo Delhon, Shi-Hua Xiang, Asit K. Pattnaik, David Steffen, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

Coxsackievirus B3 (CVB3), is commonly implicated in myocarditis, which can lead to dilated cardiomyopathy, in addition to causing acute pancreatitis and meningitis. Yet, no vaccines are currently available to prevent this infection. Here, we describe the derivation of a live attenuated vaccine virus, termed mutant (Mt) 10, encoding a single amino acid substitution H790A within the viral protein 1, that prevents CVB3 infection in mice and protects from both myocarditis and pancreatitis in challenge studies. We noted that animals vaccinated with Mt 10 developed virus neutralizing antibodies, predominantly containing IgG2a and IgG2b, and to a lesser extent IgG3 and IgG1. …

Contemporary Challenges And Opportunities For The Management Of Bird Damage At Field Crop Establishment, Christophe Sausse, Alice Baux, Michel Bertrand, Elsa Bonnaud, Sonia Canavelli, Alexandra Destrez, Page E. Klug, Lourdes Olivera, Ethel Rodriguez, Guilllermo Tellechea, Sebastian Zuil

United States Department of Agriculture Wildlife Services: Staff Publications

Bird damage, from sowing to crop establishment, is an important issue for farmers in many parts of the world. However, reliable and cost-effective solutions remain elusive because management tools and research on the subject are limited. The spatial variability of damage across landscapes and the adaptative behaviour of birds create further challenges. Additionally, the issue must be tackled at the landscape scale and involve a variety of stakeholders with conflicting interests and objectives. We summarize some of the challenges and opportunities identified to face these difficulties and address four major research directions for operational solutions including 1) crop damage assessment, …

A Systematic Review Of Recommendations For Behavioral Health Services For Transgender And Gender Diverse Adults: The Three-Legged Stool Of Evidence-Based Practice Is Unbalanced, Natalie Holt, Allura L. Ralston, Debra A. Hope, Richard Mocarski, Nathan Woodruff

Trans Collaborations Academic Papers

There is a growing literature of clinical recommendations for transgender and gender diverse (TGD) affirming behavioral health care, yet it is unknown to what extent these recommendations are rooted in evidence-based practice (EBP). This systematic review included 65 articles published between 2009 and 2018 with recommendations for behavioral health services with TGD adults, emphasizing general clinical care. Coded variables included type of article, participant demographics, aspects of EBP, and whether care was informed by objective assessment. Most articles did not equally draw from all components of EBP. Recommendations for specific clinical problems are increasingly available and address diversity within TGD …

Incompatibility Group I1 (Inci1) Plasmids: Their Genetics, Biology, And Public Health Relevance, Steven L. Foley, Pravin R. Kaldhone, Steven C. Ricke, Jing Han

United States Food and Drug Administration: Publications

Bacterial plasmids are extrachromosomal genetic elements that often carry antimicrobial resistance (AMR) genes and genes encoding increased virulence and can be transmissible among bacteria by conjugation. One key group of plasmids is the incompatibility group I1 (IncI1) plasmids, which have been isolated from multiple Enterobacteriaceae of food animal origin and clinically ill human patients. The IncI group of plasmids were initially characterized due to their sensitivity to the filamentous bacteriophage If1. Two prototypical IncI1 plasmids, R64 and pColIb-P9, have been extensively studied, and the plasmids consist of unique regions associated with plasmid replication, plasmid stability/maintenance, transfer machinery apparatus, single-stranded DNA …

Promise And Complexity Of Lupus Mouse Models, Erica Moore, Joshua A. Reynolds, Anne Davidson, Stefania Gallucci, Laurence Morel, Deepak A. Rao, Howard A. Young, Chaim Putterman

United States Public Health Resources

As a follow up to a 2010 meeting deliberating on the benefits of studying mouse models of systemic lupus erythematosus (SLE), the virtual conference “Mouse models of lupus 10 years later” convened on 10 December 2020 to address a challenging decade that saw few new therapies approved, despite leaps in knowledge

Abnormalities in both innate and adaptive immunity characterize SLE, a systemic autoimmune disease with potentially severe consequences in patients. Treatment has traditionally been limited to broad-acting, side-effect-heavy immunosuppressants, which are also incompletely effective. Thus, the need for specific therapies targeting the pathogenic mechanisms of SLE remains unmet. Affirming the …

Comparing Management Programs To Reduce Red–Tailed Hawk Collisions With Aircraft, Brian Washburn, Craig K. Pullins, Travis L. Guerrant, Gregory J. Martinelli, Scott F. Beckerman

United States Department of Agriculture Wildlife Services: Staff Publications

Wildlife-aircraft collisions (wildlife strikes) pose a serious safety risk to aircraft. Raptors (i.e., hawks and owls) are one of the most frequently struck guilds of birds within North America. Although raptors (most notably red-tailed hawks [Buteo jamaicensis]) are commonly managed at most airports and military bases, there is no scientific information available regarding comparisons of the efficacy of raptor management programs for reducing raptor-aircraft collisions. Therefore, we conducted a study to examine the efficacy of 2 raptor hazard management programs implemented at Chicago's O'Hare International Airport (ORD). The first raptor management program (Phase I) occurred during January 2010–June 2013 and …

James Davidson Fawcett (1933–2020): Imbibing With The Kiwi, Louis A. Somma

Papers in Herpetology

An obituary and summary of the life of James D. Fawcett (1933-2020), herpetologist and instructor and professor of Biology at University of Nebraska at Omaha, 1972-2015. Includes bibliography of his works, list of master's theses chaired, and recollections of former students.

Peanut Allergen Reaction Thresholds During Controlled Food Challenges In 2 Canadian Randomized Studies (Canada-Arm1 And Pisces), Derek K. Chu, Joseph L. Baumert, Steve L. Taylor, Julie A. Nordlee, Tina Nham, Jonathan Bramson, Sébastien La Vieille, Michael A. Abbott, Paul Spill, Andrea Marrin, Manel Jordana, Susan Waserman

Department of Food Science and Technology: Faculty Publications

In 2 randomized studies addressing peanut allergy (Canada-Food Allergy Risk Management 1 [NCT01812798] and Peanut Immunotherapy Starting in Canada, Evaluation and DiScovery [NCT0 1601522]), we quantified peanut allergen thresholds to food challenge using Bayesian stacked model averaging to inform policy and clinical practice. About 50% of patients tolerated more than 70 mg (~ ¼ peanut).

Overview Of The Microbiome Among Nurses Study (Micro-N) As An Example Of Prospective Characterization Of The Microbiome Within Cohort Studies, Christine Everett, Chengchen Li, Jeremy E. Wilkinson, Long H. Nguyen, Lauren J. Mciver, Kerry Ivey, Jacques Izard, Natalia Palacios, A. Heather Eliassen, Walter C. Willett, Alberto Ascherio, Qi Sun, Shelley S. Tworoger, Andrew T. Chan, Wendy S. Garrett, Curtis Huttenhower, Eric B. Rimm, Mingyang Song

Department of Food Science and Technology: Faculty Publications

A lack of prospective studies has been a major barrier for assessing the role of the microbiome in human health and disease on a population-wide scale. To address this significant knowledge gap, we have launched a large-scale collection targeting fecal and oral microbiome specimens from 20,000 women within the Nurses’ Health Study II cohort (the Microbiome Among Nurses study, or Micro-N). Leveraging the rich epidemiologic data that have been repeatedly collected from this cohort since 1989; the established biorepository of archived blood, urine, buccal cell, and tumor tissue specimens; the available genetic and biomarker data; the cohort’s ongoing follow-up; and …

Alfaxalone Successfully Immobilizes Small Indian Mongooses (Urva Auropunctata): A Field Report, Caroline C. Sauvé, Yves Rondenay, Are R. Berentsen, Mel J. Rivera-Rodriguez, Patrick A. Leighton

United States Department of Agriculture Wildlife Services: Staff Publications

We investigated intramuscular administration of alfaxalone (5.3–10.0 mg/kg) as an immobilizing agent in free-ranging small Indian mongooses (Urva auropunctata) on the island of St. Kitts, West Indies. From 5–11 January 2020, we successfully immobilized 10 of 11 mongooses. Alfaxalone provided rapid onset (median = 3 min) of immobilization. Mean duration of immobilization was 16.8 ± 3 min. Mean recovery time was 5.6 ± 1 min. There was no effect of dose administered on induction, anesthesia, or recovery times at the dose range used. We concluded that alfaxalone represents an effective alternative to dissociative agents for the immobilization of free-ranging mongooses. …

Strategic Testing Approaches For Targeted Disease Monitoring Can Be Used To Inform Pandemic Decision-Making, James D. Nichols, Tiffany L. Bogich, Emily Howerton, Ottar N. Bjørnstad, Rebecca K. Borchering, Matthew Ferrari, Murali Haran, Christopher Jewell, Kim M. Pepin, William J.M. Probert, Juliet R.C. Pulliam, Michael C. Runge, Michael Tildesley, Cecile Viboud, Katriona Shea

United States Department of Agriculture Wildlife Services: Staff Publications

More than 1.6 million Servere Acute Respiratory Syndrome Cronovirus 2(SARS-COV-2)tests were administered daily in the United States at the peak of the epidemic, with a significant focus on individual treatment. Here, we show that objective-driven, strategic sampling designs and analyses can maximize information gain at the population level, which is necessary to increase situational awareness and predict, prepare for, and respond to a pandemic, while also continuing to inform individual treatment. By focusing on specific objectives such as individual treatment or disease prediction and control (e.g., via the collection of population- level statistics to inform lockdown measures or vaccine rollout) …

An Evaluation Of Body-Grip Trap Trigger Configurations For Reducing River Otter Take Incidental To Beaver Trapping, Alec J. Sundelius, Nicholas Forman, Nathan M. Roberts, Shawn T. Rossler, David B. Ruid, Robert C. Willging

United States Department of Agriculture Wildlife Services: Staff Publications

River otter (Lontra canadensis) populations in North America have been the focus of significant restoration efforts. Wildlife management agencies, concerned about the unintentional take of river otters incidental to beaver (Castor canadensis) trapping, may recommend techniques to avoid capturing river otters. River otter avoidance techniques that are ineffective or diminish trap performance for beavers are undesirable. We conducted a field evaluation in 2015 and 2016 in Wisconsin to assess how two trigger configurations (offset and center) on body-grip traps would affect the incidental capture rate of river otters during beaver trapping. We also evaluated effects of …

Brown Treesnake Mortality After Aerial Application Of Toxic Baits, Scott M. Goetz, Eric T. Hileman, Melia G. Nafus, Amy A. Yackel Adams, Amanda R. Bryant, Robert N. Reed, Shane R. Siers

United States Department of Agriculture Wildlife Services: Staff Publications

Quantitative evaluation of control tools for managing invasive species is necessary to assess overall effectiveness and individual variation in treatment susceptibility. Invasive brown treesnakes (Boiga irregularis) on Guam have caused severe ecological and economic effects, pose a risk of accidental introduction to other islands, and are the greatest impediment to the reestablishment of extirpated native fauna. An aerial delivery system for rodent‐based toxic baits can reduce brown treesnake abundance and heterogeneity among individuals may influence bait attraction or toxicant susceptibility. Previous baiting trials have either been simulated aerial treatments or relied on slightly different bait capsule compositions and the results …

Component Causes Of Infectious Bovine Keratoconjunctivitis—Non-Moraxella Organisms In The Epidemiology Of Infectious Bovine Keratoconjunctivitis, John Dustin Loy, Kristin A. Clothier, Gabriele Maier

School of Veterinary and Biomedical Sciences: Faculty Publications

• Non-Moraxella organisms are associated with infectious bovine keratoconjunctivitis (IBK).

• Mycoplasma bovoculi can cause conjunctivitis and has a potential role in IBK pathogenesis.

• Other Non-Mycoplasma agents can cause disease that resembles IBK but is clinically different.

• Genomics and molecular technology are advancing research in this area.

• Classification and determination of pathogenesis potential of these organisms may be better understood through metagenomics and whole genome sequencing.

Complexity Of Malaria Transmission Dynamics In The Brazilian Atlantic Forest, Ana Maria Ribeiro De Castro Duarte, Licia Natal Fernandes, Fabiana Santos Silva, Igor Lucoves Sicchi, Luis Filipe Mucci, Izilda Curado, Aristides Ferrnandes, Antônio Ralph Medeiros-Sousa, Walter Ceretti-Junior, Mauro Toledo Marrelli, Eduardo Evangelista, Renildo Teixeira, Juliana Laurito Summa, Marcello Schiavo Nardi, Margoth Ramos Garnica, Ana Carolina Loss, Julyana Cerqueira Buery, Crispim Cerutti Jr., M. Andreína Pacheco, Ananias A. Escalante, Maria Anice Mureb Sallum, Gabriel Zorello Laporta

Harold W. Manter Laboratory of Parasitology: Library Materials

Plasmodium malariae and Plasmodium vivax are protozoan parasites that can cause malaria in humans. They are genetically indistinguishable from, respectively, Plasmodium brasilianum and Plasmodium simium, that is, parasites infecting New World non-human primates in South America. In the tropical rainforests of the Brazilian Atlantic coast, it has long been hypothesized that P. brasilianum and P. simium in platyrrhine primates originated from P. malariae and P. vivax in humans. A recent hypothesis proposed the inclusion of Plasmodium falciparum into the transmission dynamics between humans and non-human primates in the Brazilian Atlantic tropical rainforest. Herein, we assess the occurrence of human …

Landscape Genetics Of American Beaver In Coastal Oregon, Clinton W. Epps, Vanessa M. Petro, Tyler G. Creech, Rachel S. Crowhurst, Matthew J. Weldy, Jimmy D. Taylor

United States Department of Agriculture Wildlife Services: Staff Publications

American beaver (Castor canadensis) have been translocated for population restoration, reduction of human‐wildlife conflict, and enhancement of ecosystem function. Yet few studies have assessed dispersal of beaver, making it difficult to determine at what scale translocations are appropriate. Genetic studies can provide inferences about gene flow, and thus dispersal. We used a landscape genetic approach to evaluate whether landscape features influenced gene flow among beaver in the Coast Range of western Oregon, USA, using samples collected April–September 2014. We collected genetic samples from live‐captured (n=232), road‐killed (n=2) and trapper‐provided (n=58) tissue samples and genotyped them at 10 microsatellite loci. We …

Genome Sequence Of A Virulent African Swine Fever Virus Isolated In 2020 From A Domestic Pig In Northern Vietnam, Quang Lam Truong, Thi Lan Nguyen, Thi Hoa Nguyen, Jishu Shi, Hiep Lai Xuan Vu, Thi Lan Huong Lai, Van Giap Nguyen

Nebraska Center for Virology: Faculty Publications

This study reports the genome sequence of an isolated African swine fever (ASF) virus (VNUA-ASFV-05L1/HaNam) obtained at the fourth passage on pulmonary alveolar macrophages. The virus was isolated during a typical acute ASF outbreak in pigs in a northern province of Vietnam in 2020.

Short Term Safety, Immunogenicity, And Reproductive Effects Of Combined Vaccination With Anti-Gnrh (Gonacon) And Rabies Vaccines In Female Feral Cats, Shiri Novak, Boris Yakobson, Shir Sorek, Liat Morgan, Smadar Tal, Ran Nivy, Roni King, Lauren Jaebker, Douglas C. Eckery, Tal Raz

United States Department of Agriculture Wildlife Services: Staff Publications

Overpopulation of free-roaming cats is a major problem leading to negative impacts on animal health and welfare, public nuisance, transmission of zoonotic diseases, and well-documented harm to wildlife. Surgical sterilization had failed to provide a practical solution to free-roaming cats’ overpopulation under field conditions; therefore, efficient and safe non-surgical immunocontraception methods are aspired. Rabies is a deadly virus that may infect people and animals. However, the safety and efficacy of combined vaccination with anti-GnRH and rabies vaccines in feral cats, which often suffer from disrupted health conditions and experienced high stress level, has never been studied. Therefore, our objective was …

Short Term Safety, Immunogenicity, And Reproductive Effects Of Combined Vaccination With Anti-Gnrh (Gonacon) And Rabies Vaccines In Female Feral Cats, Shiri Novak, Boris Yakobson, Shir Sorek, Liat Morgan, Smadar Tal, Ran Nivy, Roni King, Lauren Jaebker, Douglas C. Eckery, Tal Raz

United States Department of Agriculture Wildlife Services: Staff Publications

Overpopulation of free-roaming cats is a major problem leading to negative impacts on animal health and welfare, public nuisance, transmission of zoonotic diseases, and well-documented harm to wildlife. Surgical sterilization had failed to provide a practical solution to free-roaming cats' overpopulation under field conditions; therefore, efficient and safe non-surgical immunocontraception methods are aspired. Rabies is a deadly virus that may infect people and animals. However, the safety and efficacy of combined vaccination with anti-GnRH and rabies vaccines in feral cats, which often suffer from disrupted health conditions and experienced high stress level, has never been studied. Therefore, our objective was …

Codon Bias Can Determine Sorting Of A Potassium Channel Protein, Anja J. Engel, Marina Kithil, Markus Langhans, Oliver Rauh, Matea Cartolano, James L. Van Etten, Anna Moroni, Gerhard Thiel

Nebraska Center for Virology: Faculty Publications

Due to the redundancy of the genetic code most amino acids are encoded by multiple synonymous codons. It has been proposed that a biased frequency of synonymous codons can affect the function of proteins by modulating distinct steps in transcription, translation and folding. Here, we use two similar prototype K⁺ channels as model systems to examine whether codon choice has an impact on protein sorting. By monitoring transient expression of GFP-tagged channels in mammalian cells, we find that one of the two channels is sorted in a codon and cell cycle-dependent manner either to mitochondria or the secretory pathway. …

The Experts Speak: A New Feature In The Jicr, Michael Gale, Ram Savan, Adolfo Garcia-Sastre, Ludmila Prokunina-Olsso, Kuppusamy Balamurugan, Howard A. Young

United States Public Health Resources

This month we launch a new feature in the JICR: The Experts Speak. This feature is designed to provide first-hand insight from topical experts in the fields of interferon, cytokines, innate immunity, and adaptive immune actions of cytokine biology in disease. We start by asking experts to comment on interferon, cytokines, and SARS-CoV-2.

Component Causes Of Infectious Bovine Keratoconjunctivitis - The Role Of Moraxella Species In The Epidemiology Of Infectious Bovine Keratoconjunctivitis, John Dustin Loy, Matthew Hille, Gabriele Maier, Michael L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

Moraxella bovis can cause infectious bovine keratoconjunctivitis (IBK).

The role of M bovoculi in IBK is not fully understood.

M bovis and M bovoculi appear to undergo genetic recombination with each other or other members of the Moraxellaceae.

Recombination complicates their classification and potential role(s) in IBK pathogenesis.

MALDI-TOF MS is used to identify M bovis, 2 major strains or genotypes of M bovoculi, M ovis, and other members of the Moraxellaceae.

Classification and determination of pathogenesis potential within Moraxella species may be better understood through whole genome sequencing.