Medicine and Health Sciences Commons^™

Production Of Shiga-Like Toxin By Escherichia Coli, Lilian R. M. Marques, Marjorie A. Moore, Joy G. Wells, I. Kate Wachsmuth, Alison D. O'Brien

Uniformed Services University of the Health Sciences

Escherichia coli is one of several agents that cause intestinal disease in humans and animals. Four classes of E. coli have been recognized [1], Enterotoxigenic E. coli (ETEC) strains produce a heat-labile (LT) enterotoxin and/or a heat-stable( ST) enterotoxin. Enteroinvasive E . coli (EIEC) strains, like shigellae, penetrate and multiply within epithelial cells. Enteropathogenic E. coli (EPEC) strains belong to certain serogroups that have been incriminated as pathogens by epidemiological studies. Some EPEC strains have been shown to adhere to cells of the intestinal mucosa and to produce pathognomonic lesions at the site of attachment. Enterohemorrhagic E. coli (EHEC) strains …

Formation Of Influenza Virus Particles Lacking Hemagglutinin On The Viral Envelope, Asit K. Pattnaik, Donald J. Brown, Debi P. Nayak

School of Veterinary and Biomedical Sciences: Faculty Publications

We investigated the intraceUular block in the transport of hemagglutinin (HA) and the role of HA in virus particle formation by using temperature-sensitive (Is) mutants (1s134 and 1s61S) of inOuenza virus AlWSN/33. We found that at the nonpermissive temperature (39.5°C), the exit of ts HA from the rough endoplasmic reticulum to the Golgi complex was blocked and that no additional block was apparent in either the exit from the Golgi complex or post-Golgi complex transport. When MDBK ceUs were infected with these mutant viruses, they produced noninfectious virus particles at 39.5°C. The efficiency of particle formation at 39.5°C was essentiaUy …

Results Of A Head And Neck Examination: Evaluation Of A Tumor Of The Floor Of The Mouth, Caren M. Barnes, Kenneth W. Nelson, Gary H. Conover

College of Dentistry: Faculty Publications

This article reviews a solitary, well-defined tumor of the floor of the mouth. The tumor presented as a raised, elevated, sessile mass that was freely movable and soft to. the touch. The case illustrates the importance of performing a thorough head and neck examination, and formulating a differential diagnosis if any abnormality is detected. The clinical features, histopathology and responsibility of the dental hygienist in performing a thorough head and neck examination is reviewed.

Soybean Oil Is Not Allergenic To Soybean-Sensitive Individuals, Robert K. Bush, Steve L. Taylor, Julie A. Nordlee, William W. Busse

Department of Food Science and Technology: Faculty Publications

We have previously demonstrated that peanut oil is not allergenic to peanut-sensitive individuals. Seven soybean-sensitive patients were enrolled in a double-blind crossover study to determine whether ingestion of soybean oil can induce adverse reactions in such patients. All subjects had histories of systemic allergic reactions (urticaria, angioedema, wheezing, dyspnea, and/or vomiting) after soybean ingestion and had positive puncture skin tests with a 1:20 w/v glycerinated-saline whole soybean extract. Sera from six of the seven subjects were tested by RAST assay for the presence of specific IgE antibodies to soybean allergens. All patients had elevated levels of serum IgE antibodies to …

Recovery Of Helminths From Prehistoric Feces: The Cultural Ecology Of Ancient Parasitism, Karl J. Reinhard

Karl Reinhard Publications

Several helminth parasites have been recovered from archaeological sites on the Colorado Plateau. These include Ascaris, Trichuris, Enterobius, Moniliformis, and tapeworms in the families Hymenolepidae and Taeniidae. The recovery of these animals demonstrates that cross-temporal: comparison of parasitism between different prehistoric lifestyles is possible. Such comparison might elucidate the effects of changing lifestyle and subsistance pattern on human parasitism.

Desiccated feces recovered from archaelogical deposits were studied. Hunter-gatherer feces were selected from Dust Devil Cave in southeastern Utah. Agriculturalist feces were studied from Antelope House, a cave site in northeastern Arizona, and Salmon Ruins, a Pueblo …

Pubertal Timing And Grade Effects On Adjustment, Anne C. Petersen, Lisa J. Crockett

Department of Psychology: Faculty Publications

Effects on adjustment of biological maturation and social timing were com pared using data from a longitudinal sample of 335 young adolescents, who were followed from the sixth through eighth grades. Biological maturation was estimated from the relative timing of the adolescent growth spurt. Social timing was assessed by grade in school. Five adjustment constructs were examined: school achievement (course grades in five subjects), family relations (a 17-item scale), peer relations (a 10- item scale), body image (an 11-item scale), impulse control (an 8-item scale), and psychopathology (an 11-item emotional tone scale and an 11-item general psychopathology scale). All but …

Rationality, Culture, And The Construction Of “Ethical Discourse”: A Comparative Perspective , Carolyn P. Edwards

Department of Psychology: Faculty Publications

The problem of ethical relativism has never been resolved or laid to rest. It turns out to be a complicated set of problems, involving many philosophical issues of meaning (Brandt 1954; Ladd 1957). For example, how should we define morality and ethics? How should we define the problem of ethical relativism? How does the problem of ethical relativism relate to the problem of cultural relativism?

One question that is part of this package is a scientific one and concerns whether there are even aspects of moral values and ethical discourse that can be validly abstracted from their cultural context and …

Characterization Of Monoclonal Antibodies Against Shiga-Like Toxin From Escherichia Coli, Nancy A. Strockbine, Lilian R. M. Marques, Randall K. Holmes, Alison D. O'Brien

United States Public Health Resources

Three monoclonal antibodies, designated MAb 16E6, MAb 13C4, and MAb 19G8, were produced which recognize Shiga-like toxin (SLT) from Escherichia coli. All three monoclonal antibodies neutralized the cytotoxicity of E. coli SLT and were able to immunoprecipitate intact labeled toxin with Staphylococcus aureus protein A. The three antibodies were of the Gl heavy and kappa light chain classes. MAb 16E6 bound to the B subunit of SLT in Western blots and also neutralized the lethality of the toxin for mice and the enterotoxicity of the toxin in ligated rabbit ileal loops. The ability of MAb 16E6 to neutralize the …

The Diarrheal Response Of Humans To Some Classic Serotypes Of Enteropathogenic Escherichia Coli Is Dependent On A Plasmid Encoding An Enteroadhesiveness Factor, Myron M. Lavine, James P. Nataro, Helge Karch, Mary M. Baldini, James B. Kaper, Robert E. Black, Mary Lou Clements, Alison D. O'Brien

Uniformed Services University of the Health Sciences

Isolates of the most common 0 serogroups of enteropathogenic Escherichia coli (EPEC) associated with infant diarrhea (designated class I) adhere to Hep-2 cells; the genes for this adhesin, termed EPEC adherence factor (EAF), are located on plasmids 50-70 MDa in size. Volunteers ingested 10¹⁰ organisms of an 0127:H6 Hep-2-adhesive class I strain (E2348/69) or its plasmid-minus, nonadhesive derivative. Diarrhea occurred in nine of 10 volunteers who ingested the parent strain (mean, 1,178 ml) but in only two of nine who took the plasmid-minus variant (mean, 433 ml; P < .006). All volunteers ill from strain E2348/69 mounted serum IgA and IgG responses to a 94-kDa plasmid-associated outer membrane protein of E2348/69; this protein was found in other class I EPEC but not in enterotoxigenic or meningitic strains. The 50-70-MDa EAF plasmid seems neces- sary for full expression of pathogenicity in EPEC that exhibit Hep-2 adhesiveness. EPEC isolates of certain other, less common, 0 serogroups (044, 086, and 0114) are rarely Hep-2 adhesive. These EPEC, designated class II, possess distinct 50-70 MDa plasmids lacking EAF genes. Diarrhea was caused by 10⁸ or 10¹⁰ organisms of an 0114:H2 class II EPEC …

Effects Of Interpersonal Problem-Solving Training With Chronic Aftercare Patients On Problem-Solving Component Skills And Effectiveness Of Solutions, David J. Hansen, Janet S. St. Lawrence, Karen A. Christoff

Department of Psychology: Faculty Publications

Seven chronic psychiatric patients who were enrolled in a partial-hospitalization program and had difficulty solving interpersonal problems received group skills training. A multiple-baseline design was used to demonstrate training effectiveness through improved problem solving on trained situations and through skill generalization to novel, untrained situations. Maintenance of the training was evaluated after 1 month and 4 months. Social validation was accomplished by comparing the problem-solving competency of the patient sample with skills demonstrated by “normal” nonpsychiatric persons in their community.

Modification Of Sand Fly Biting Behavior By Leishmania Leads To Increased Parasite Transmission, Raymond Beach, Gabriel Kjilu, Johannis Leeuwenberg

Harold W. Manter Laboratory of Parasitology: Library Materials

To attempt rodent-sand fly-rodent transmission of Leishmania major, laboratory-reared Phlebotomus doboscqi were fed on L. major-infected mice and then refed on uninfected mice 21 days later. Flies which refed either probed 1–2 times and took a full blood meal in less than 10 minutes or probed 3 or more times and took little or no blood during a period of 15 minutes or more. When dissected, 7 of 8 flies which experienced difficulty in obtaining a blood meal had flagellates in their cibaria, an observation supporting the hypothesis that parasites in this part of the alimentary canal modify normal …

Sulfite Residues In Maraschino Cherries, Julie A. Nordlee, Laura B. Martin, Steve L. Taylor

Department of Food Science and Technology: Faculty Publications

A survey of 53 samples of maraschino cherries from 14 different processors revealed that total sulfite residues averaged 52.3 ± 44.7 ppm. However, 58.5% of the samples had less than 40 ppm total SO₂, while only 7.6% had greater than 120 ppm total SO₂ indicating that the distribution was skewed in the direction of lower residue levels. Free sulfite residues in the 53 samples were considerably lower, averaging 14.2 ± 7.1 ppm. With free SO₂ levels, 35.8% of the samples had less than 10 ppm free SO₂, while only 5.7% had greater than 30 …

How Does Familiarity Affect Visual Search For Letter Strings?, John H. Flowers, Doris J. Lohr

Department of Psychology: Faculty Publications

Subjects visually searched for letter string targets consisting of either familiar English three-letter words (e.g., SEX) or featurally similar nonword trigrams (e.g., SFX). Distractor items were either words or nonwords and had varying degrees of feature overlap with the target among different blocks of trials. Search was facilitated by a word-nonword category distinction between target and distractors, particularly when target-noise feature overlap was high, but such facilitation consisted of slope reductions in an apparently serial, self-terminating search pattern as opposed to a "pop-out" effect.

Namru-3 Translations Of Parasite Literature From Russian, French, Japanese, Etc. Into English (1929-1985) T1701-T1800, United States Naval Medical Research Unit Number Three, Cairo, Medical Zoology Department

United States Naval Medical Research Unit 3: Publications

T1701. TRANSLATION FROM RUSSIAN, OVEZMUKHAMMEDOV, A. and SAF'YANOVA, V. M. (1983), Occurrence of amastigotes of Leishmania gymnodactyli in the peripheral blood of Gymnodactylus caspius and Agama caucasica. Parazitologiya, Leningrad. 17(3):185-188.

T1702. TRANSLATION FROM RUSSIAN. YASTREBOV, V. K. and YURLOV, K. T. (1978). Possible arbovirus dissemination in western Siberia in connection with data on migrations and overwinterings of west Siberian birds. Dokl. Simp. Transkont. Svyazi Pereletn. Ptits Rol' v Rasp. Arbovirus. (Novosibirsk, July-August, 1976), pp. 167-172.

T1703. TRANSLATION FROM GERMAN. MOSKVIN, I. A. (1929). On the role of the tick Ornithodoros papillipes Bir. (Ixodoidea) in transmitting relapsing fever in Turkestan. Z. …

The Role Of Emotion In Moral Socialization, Richard A. Dienstbier

Department of Psychology: Faculty Publications

The major aim of this chapter is to discuss a research program designed to assess emotion-cognition-behavior relationships in moral decision making. While pursuing that aim, I will discuss the relationship of that research to some of the more enduring theoretical issues in emotion.

Theories of emotion wax and wane across a more extended time frame than is typical for theoretical approaches in many other areas in psychology. Perhaps these extended cycles of emotion theory result from the relative difficulty in the recent past in doing definitive research within the area of human emotion. The field was thereby insulated from sudden …

Effect Of Membrane Filtration Of Antimalarial Drug Solutions On In Vitro Activity Against Plasmodium Falciparum, J. Kevin Baird, C. Lambros

United States Public Health Resources

Antimalarial activities ofchloroquine, mefloquine, amodiaquine, and quinine in vitro against Plasmodium falciparum were diminished as a consequence ofmembranefiltration. Filtered drug solutions gave ID₅₀ values up to 25-fold greater than those of non-filtered (ethanol-sterilized) drug solutions. Loss ofactivity byfiltration was overcome by increasing the drug concentration prior to filtration. Water solutions filtered through Millex-GSfilter units consistently showed an absorbance maximum at 277 nm, accompanied by a lesser peak at 225 nm. Water filtrates from Nucleopore and Millex-G Vfilters showed no absorbance at 277 nm and only slight absorbance was evidentfor the Gelmanfilter unit. Activity losses were attributed to extractable contaminating …

Namru-3 Translations Of Parasite Literature From Russian, French, Japanese, Etc. Into English (1973-1984) T1801-T1900, United States Naval Medical Research Unit Number Three, Cairo, Medical Zoology Department

United States Naval Medical Research Unit 3: Publications

T1801. TRANSLATION FROM RUSSIAN. KOLOTVINOV, S. V. and MASLENNIKOV, V. A. (1980). Method for evaluating the functional activity of viral antigens. Zh. Mikrobiol., Moskva, 57(8): 58-61.

T1803. TRANSLATION FROM GERMAN. CAFLISH, U., TONZ, O., SCHAAD, U. B., AESCHLIMANN, A. and BURGDORFER, W. (1984). Tick-borne meningo-radiculitis – a spirochetosis. Schweiz. Med. Wschr., 114(18):630-634.

T1804. TRANSLATION FROM FRENCH. LEW, D. (1984). Fevers developing after travels – some unusual cases. Schweiz. Med. Wschr., 114(29):1029-1031.

T1805. TRANSLATION FROM RUSSIAN. L'VOV, D. K., ANDREEV, V. P., ISACHENKO, V. A., YAKHNO, M. A. ZAKSTEL'SKAYA, L. Ya., SOKOLOVA, N. N. and VASIL' EV, V. I. (1974). Detection …

Identification Of Four Complementary Rna Species In Akabane Virus-Infected Cells, Asit K. Pattnaik, G. Abraham

School of Veterinary and Biomedical Sciences: Faculty Publications

The analysis of RNA extracted from purified Akabane virus demonstrated the presence of three size classes of single-stranded RNAs with sedimentation coefficients of 31S (large, L), 26S (medium, M), and 13S (small, S). Molecular weights of these RNA species were estimated to be 2.15 x 10⁶,1.5 X 10⁶, and 0.48 x 10⁶ for the L, M, and S RNAs, respectively. Hybridization analysis involving viral genomic RNA and RNA from virus-infected cells resulted in the identification of four virus-specific cRNA species in infected cells. These cRNAs were found to be nonpolyadenylated by their inability to bind …

Field Technique For The Identification Of Deer Blood, David W. Oates, Carol A. Jochum, Kenneth A. Pearson, Cathy A. Hoilien

Nebraska Game and Parks Commission: Staff Research Publications

A latex suspension sensitized with deer antiserum has been prepared, placed on plastic cards, dried, and packaged for field use. The product was tested against bloodstains from 22 species (including Homo sapiens). Strong agglutination reactions occurred only with bloodstains from deer and elk.

Escherichia Coli Strains Producing Streptococcus Mutans Proteins Responsible For Colonization And Virulence, Roy Curtiss Iii, Robert G. Holt, Raul G. Barletta, James P. Robeson, Shigeno Saito

School of Veterinary and Biomedical Sciences: Faculty Publications

Streptococcus mutans is a principal etiologic agent of dental caries and is likely one of the most ubiquitous bacterial infectious disease agents worldwide. The ability of S. mutans to colonize the oral cavity is due to sucroseindependent and sucrose-dependent adherence to the pellicle-coated tooth surface with glucan facilitated aggregation between cells to result in plaque. Cariogenicity is then caused by the ability of S. mutans in plaque to metabolize free sugars and both extra and intracellular complex carbohydrates to yield predominantly lactic acid. The S. mutans gene products that contribute to colonizing ability and thus virulence include glucosyltransferases, glucanbinding proteins, …

Infectious Pancreatic Necrosis (Ipn) Of Salmonid Fishes, Phillip E. Mcallister

United States Fish and Wildlife Service: Publications

Infectious pancreatic necrosis (IPN) is a viral disease principally associated with salmonids, although IPN and lPN-like viruses have been isolated from various nonsa1rnonid fish and marine invertebrates. Acute infection occurs in 1- to 4-month-old salmonids and can result in cumulative mortality approaching 100%. In contrast, fish 6 months old or older can undergo subclinical or inapparent infection, but experience no significant mortality. Survivors of the disease can become lifelong carriers of the virus. Epizootics of IPN have been reported worldwide, and several virus serotypes are recognized. The disease known as IPN was probably first described by M'Gonigle (1941) as acute …

A Balb/C Congenic Strain Of Mice That Carries A Genetic Locus (ItyR) Controlling Resistance To Intracellular Parasites, Michael Potter, Alison D. O'Brien, Emil Skamene, Philippe Gros, Adrien Forget, Patricia A. L. Kongshavn, Judith S. Wax

Uniformed Services University of the Health Sciences

BALB/c.DBA/2 Idh-1^b-Ity^r-Pep-3^b congenic mice were developed by introgressively backcrossing the Idh-1^b and Pep-3^b markers of DBA/2 mice onto the BALB/cπ mice. This introduced a 30-centimorgan chromosome 1 segment of DBA/2 chromatin that contained the Ity^r gene. BALB/c.DBA/2 Idh-1^b-Ity^r-Pep-3^b mice were resistant to in vivo infections by Salmonella typhimurium, Mycobacterium bovis, and Leishmania donovani.

Purification And Characterization Of A Shigella Dysenteriae 1- Like Toxin Produced By Escherichia Coli, Alison D. O'Brien, Gerald D. Laveck

Uniformed Services University of the Health Sciences

A toxin from an enteropathogenic strain of Escherichia coli (E. coli H30) was purified to apparent homogeneity from cell lysates. The steps used to isolate the E. coli H30 toxin included French pressure-cell disruption of bacteria grown in iron-depleted media, Affi-Gel Blue chromatography, chromatofocusing, and anti- Shiga toxin affinity chromatography. The mobilities of the subunits of radioiodinated E. coli H30 toxin and Shiga toxin observed after the two toxins were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis were identical. In the absence of 2-mercaptoethanol, a narrow band was seen at M_r 31,500 (±1,000), and a wide heavy band …

31P Nmr Study Of Erythrocytes From A Patient With Hereditary Pyrimidine-5'-Nucleotidase Deficiency, M. S. Swanson, C. R. Angle, S. J. Stohs, S. T. Wu, J. M. Salhany, R. S. Elliot, R. S. Markin

United States Public Health Resources

The composition of phosphate metabolites and the intracellular pH in erythrocytes from a patient with hereditary pyrimidine-5'-nucleotidase deficiency were examined using ³¹P NMR spectroscopy. Several resonances were identified in spectra from intact cells and from extracts. The 2,3-bisphosphoglycerate line intensities were normal but the NTP resonances were about twice normal due to the presence of millimolar quantities of pyrimidine phosphates. Several intense resonances were also observed in the diphosphodiester region of the spectrum. One compound contributing to these lines has been identified as cytidine diphosphocholine. The resonances of NTPs were in a position indicating that the additional triphosphates were …

Expression Of A Streptococcus Mutans Glucosyltransferase Gene In Escherichia Coli, James P. Robeson, Raul G. Barletta, Roy Curtiss Iii

School of Veterinary and Biomedical Sciences: Faculty Publications

Chromosomal DNA from Streptococcus mutans strain UAB90 (serotype c) was cloned into Escherichia coli K-12. The clone bank was screened for any sucrosehydrolyzing activity by selection for growth on raffinose in the presence of isopropyl- β-D-thiogalactoside. A clone expressing an S. mutans glucosyltransferase was identified. The S. mutans DNA encoding this enzyme is a 1.73-kilobase fragment cloned into the HindIII site of plasmid pBR322. We designated the gene gtfA. The plasmid-encoded gtfA enzyme, a 55,000-molecular-weight protein, is synthesized at 40% the level of pBR322-encoded β-lactamase in E. coli minicells. Using sucrose as substrate, the gtfA enzyme catalyzes …

Namru-3 Translations Of Parasite Literature From Russian, French, Japanese, Etc. Into English (1915-1983) T1601-T1700, United States Naval Medical Research Unit Number Three, Cairo, Medical Zoology Department

United States Naval Medical Research Unit 3: Publications

T1603. TRANSLATION FROM RUSSIAN. SELIVANOV, Ya. M. and POLYAKOVA, A. N. (1981). Technic of preparing and producing experimental series of immunoglobulin erythrocytic polytype diagnosticums to certain arboviruses for the indirect hemagglutination test. Sborn. Trud. Inst. Virus. imeni D. I. Ivanovsky, Akad. Med. Nauk SSSR, pp. 145-151.

T1604. TRANSLATION FROM RUSSIAN. ROSLAYA, I. G., DEMENEV, V. A., PRYGOVA, A. I., ROSLYAKOV, G. E. and VERETA, L. A. (1981). Certain questions of ecology of tickborne encephalitis group viruses in the lower Pre-Amur'ye. Sborn. Trud. Inst. Virus. imeni D. I. Ivanovsky, Akad. Med. Nauk SSSR, pp. 90-94.

T1605. TRANSLATION FROM RUSSIAN. EMEL'YANOVA. N. …

United States Naval Medical Research Unit Number Three, Cairo, Egypt, Medical Zoology Department Translation List, Harry Hoogstraal

United States Naval Medical Research Unit 3: Publications

List of translations of medical parasitology and medical entomology papers prepared by the United States Naval Medical Research Unit Number 3 in Cairo, Egypt. The papers were translated from numerous languages, such as Russian (most were translated from Russian), Bulgarian, Chinese, Czech, Finnish, French, German, and Japanese, into English. The publication dates of the original papers ranged from about 1915 to 1983. Harry Hoogstraal translated some or all of the papers.

Microbiological Water Quality Of Impoundments: A Literature Review, G. Allen Burton Jr.

United States Public Health Resources

Assessing the microbiological water quality of impoundments and the potential for waterborne disease outbreaks is a difficult task when using traditional sampling programs. Problems associated with using fecal coliform bacteria as indicators of human pathogen presence complicates assessments of future water quality in preimpoundment areas. Reliable determination of future and present microbiological water quality requires knowledge of how the chemical, physical, and biological characteristics of the watershed and impoundment interrelate to influence microbial indicator and pathogen densities. Accurate estimates of microbial indicator and pathogen densities, obtainable by using the enumeration methods and their modifications suggested in this report, will allow …

Strange Males Block Pregnancy In Lactating Pine Voles, Microtus Pinetorum, And Reduce Survival And Growth Of Nursing Young, Margaret H. Schadler

Eastern Pine and Meadow Vole Symposia

Strange (unfamiliar) males affect survival of progeny of reproductive female rodents in a variety of ways. In mice, strange males block pregnancy before implantation of embryos (Bruce, 1959) but only in females that are not lactating (Bruce and Parkes, 1961). In voles these males can block pregnancy both before and after implantation (Stehn and Richmond, 1975; Marks and Schadler, 1979; Schadler, 1981). Strange males have also been shown to kill nursing pups in lemmings (Mallory and Brooks, 1978) and mice (Labov, 1980 and vom Saal and Howard, 1982).

The present study in pine voles (Microtus pinetorum) examines the …

Production Of Shigella Dysenteriae Type 1-Like Cytotoxin By Escherichia Coli, Alison D. O'Brien, Gerald D. Laveck, Michael R. Thompson, Samuel B. Formal

Uniformed Services University of the Health Sciences

Strains of Escherichia coli previously implicated or proven to be causes of diarrhea were examined for production of a toxin similar to that of Shigella dysenteriae type 1 (Shiga). Organisms grown in an iron-depleted broth were lysed by pressure disruption followed by ultracentrifugation. Saline-dialyzed extracts were tested for cytotoxic effects on HeLa cells that were neutralizable with antiserum to Shiga toxin. Among the 13 E. coli strains so analyzed, 11 made a Shiga-like cytotoxin in levels ranging from trace (two avirulent isolates) to amounts equivalent to S. dysenteriae type 1 (two noninvasive strains that did not make E. coli heat-labile …