Medicine and Health Sciences Commons^™

Impairment Of Melibiose Utilization In Streptococcus Mutans Serotype C Gtfa Mutants, Raul G. Barletta, Roy Curtiss Iii

School of Veterinary and Biomedical Sciences: Faculty Publications

The Streptococcus mutans serotype c gtfA gene encodes a 55-kilodalton sucrose-hydrolyzing enzyme. Analysis of S. mutans gtfA mutants revealed that the mutant strains were specifically impaired in the ability to use melibiose as a sole carbon source. S. mutans gafA mutant strains synthesized less α-galactosidase activity inducible by raffinose than wild-type strains. Melibiose (an inducer in wild-type strains) failed to induce significant levels of a-galactosidase in the mutant strains. We hypothesize that melibiose use by . mutans requires the interaction of the GtfA enzyme, or another gene product under the control of the gtfA promoter, with other gene product(s) involved …

Pig Vocalizations Under Selected Husbandry Practices, H. Xin, J. A. Deshazer, Daniel W. Leger

Department of Psychology: Faculty Publications

Acoustical characteristics of vocalizations of sows, piglets, and nursery pigs under selected husbandry practices were analyzed with a digital signal processing system. The duration (D) and major energy-resonance frequency (P) for each call were determined as follows: a) processing of piglets (D = 0.81 s and f* = 3 700 Hz); b) food anticipation of breeding-gestation sows (2.50 s and 3 000 Hz); c) isolation of piglet (0.34 s and 500, 3 500 Hz); d) startling of nursery pigs (0.29 s and 900 Hz); e) sows in heat (3.07 s and 1 375 Hz); f) farrowing (0.10 s and 3 …

From The Gulf To The Rio Grande: Human Adaptation In Central, South, And Lower Pecos Texas, Thomas Hester, Stephen L. Black, D. Gentry Steele, Ben W. Olive, Anne A. Fox, Karl Reinhard, Leland C. Bement

Karl Reinhard Publications

The South Texas area, Region 3 of the Southwestern Division, U.S. Army Corps of Engineers, is synthesized from archeological and bioarcheological perspectives. Three distinct geographic units within Region 3 are treated in detail: Central Texas Plateau Prairie, South Texas Plains, and Lower Pecos Canyonlands. More than 11,000 years of human adaptation are chronicled for this area, stretching from the Gulf of Mexico to the Rio Grande along the border with northeastern Mexico. Particular attention is devoted to a consideration of the region's prehistoric record; significant problems and data gaps are outlined. For the first time, a compilation has been done …

Toxin Genotypes And Plasmid Profiles As Determinants Of Systemic Sequelae In Escherichia Coli 0157:H7 Infections, Stephen Ostroff, Phillip Tarr, Marguerite A. Neill, Jay H. Lewi, Nancy Hargrett-Bean, John M. Kobayashi

United States Public Health Resources

In 1987, 93 Escherichia coli 0157:H7 isolates were collected during routine surveillance for this pathogen in the state of Washington. Toxin genotypes and plasmid profiles were correlated with the clinical sequelae of illness in 88 of the 93 patients from whom these strains were isolated. Thirteen plasmid patterns were observed among the 88 tested isolates; four patterns accounted for 82(7o of the isolates. Genetic probing for Shiga-like toxins (SLT) I and II demonstrated the presence of both genes in 67 (76%), SLT I alone in three (3%), and SLT II alone in 18 (20%). The hemolytic uremic syndrome or throm-botic …

In Vivo Formation Of Hybrid Toxins Comprising Shiga Toxin And The Shiga-Like Toxins And Role Of The B Subunit In Localization And Cytotoxic Activity, Debra L. Weinstein, Matthew P. Jackson, Liyanage P. Perera, Randall K. Holmes, Alison D. O'Brien

Uniformed Services University of the Health Sciences

Shiga toxin, Shiga-like toxin I (SLT-I) and Shiga-like toxin II (SLT-H) are cell-associated cytotoxins that kill both Vero cells and HeLa cells, whereas Shiga-like toxin II variant (SLT-llv) is an extracellular cytotoxin that is more cytotoxic for Vero cells than for HeLa cells. The basis for these differences in cytotoxin localization and host cell specificity were examined in this study. The A and B subunit genes of Shiga toxin and the SLTs were recombined by two methods so that hybrid toxins would be formed in vivo. Complementation of heterologous subunits was accomplished by cloning the individual A and B subunit …

Case Report Of Human Infection With Capillaria Philippinensis, Ching-Yu Chen, Wei-Chuan Hsieh, Tsang-Lie Chen

Harold W. Manter Laboratory of Parasitology: Library Materials

In July 1988, a case of human infection with Capillaria philippinensis, so-called the intestinal capillariasis, was discovered at the National Taiwan University Hospital (NTUH). This is the first case reported in Taiwan.

Capillaria philippinesis is a parasite of fish-eating birds In nature it has a fish-bird life cycle. When human populations disrupt the natural cycle by eating raw fish infected with the larvae of C. philippinensis, they may acquire the disease.

A Mimbres Burial With Associated Colon Remains From The Nan Ranch Ruin, New Mexico, Harry J. Shafer, Marrianne Marek, Karl J. Reinhard

Karl Reinhard Publications

The skeletal remains of an adult male associated with desiccated tissue and a coprolite were recovered from an apen-air midden deposit at the NAN Ranch Ruin (LA15049)) a large Mimbres site in Grant County) New Mexico. The find dates to about A.C. 1000-1100. Identifiable macroscopic elements in the caprolite consist offi nely fragmented corn and tiny seed fragments of an unknown plant. High amounts of willow (Salix) and mustard (Brassicaceae) pollen may indicate the ingestion of medicinal plants to combat a deteriorating health condition. The individual was approximately 35 -40 years old at the time of death and suffered from …

Bioarcheological Synthesis For "From The Gulf To The Rio Grande: Human Adaptation In Central, South, And Lower Pecos Texas", Karl Reinhard, Ben W. Olive, D. Gentry Steele

Karl Reinhard Publications

One of the main problems encountered in the review of the bioarcheology of Region 3 has been the limited number of sites where human skeletal material has been adequately recovered and analyzed. In the preceding chapter it was documented that less than 30% of the burials recovered from recorded sites have been reported in published literature. It was further estimated that of the 323 sites with burials, no more than 80 sites have published detailed bioarcheological reports on the burials recovered. Only 50 of these 80 reports provide individual descriptions of each burial which facilitate subsequent analyses and evaluation.

Four …

Creativity And Perception, John H. Flowers, Calvin P. Garbin

Department of Psychology: Faculty Publications

Informal thought about the nature of mental operations important to creative human behavior suggests that perceptual processes are of considerable importance. The ability to “see relationships among elements” is an attribution commonly made toward authors of major scientific discoveries or of noteworthy artistic achievements. For example, Shepard (1978, 1981) documented self-reports from several creative scientists and authors that strongly emphasize the role of visual imagery and the manipulation of visual codes in the creative process.

Given the anecdotal and self-report evidence for a relationship between creative behavior and aspects of perceptual processing, it initially may seem surprising that there is …

The Relations Of Pubertal Status To Intrapersonal Changes In Young Adolescents, Lorah D. Dorn, Lisa J. Crockett, Anne C. Petersen

Department of Psychology: Faculty Publications

The purpose of this study was to investigate the relations between pubertal development and adolescents’ perceptions of their physical and emotional states. Two research questions were posed: (a) What are the effects of pubertal status upon satisfaction with appearance and mood states of the adolescent; and, (b) What are the patterns of those pubertal effects in sixth, seventh, and eighth grades? A nonclinical group of adolescents (N = 253) was selected from two cohorts and was followed from sixth through eighth grade. Multiple measures were used to assess satisfaction with appearance and mood states. Significant multivariate findings were limited to …

Sensitivity To Sulfited Foods Among Sulfite-Sensitive Subjects With Asthma, Steve L. Taylor, Robert K. Bush, John C. Selner, Julie A. Nordlee, Matthew B. Wiener, Karen Holden, Jerald W. Koepke, William W. Busse

Department of Food Science and Technology: Faculty Publications

Eight individuals with asthma who had been diagnosed as sulfite sensitive on the basis of double-blind capsule-beverage challenges were subjected to challenges with various sulfited foods, including lettuce, shrimp, dried apricots, white grape juice, dehydrated potatoes (as mashed potatoes), and mushrooms. Four of these patients failed to respond to challenges with any of the sulfited foods. The other four patients experienced a decrease in pulmonary function on double-blind challenges with sulfited lettuce. Two of three of these patients reacted to challenges with dried apricots and white grape juice; the fourth patient has not yet been challenged with these products. Only …

Analysis Of The Virulence Of Streptococcus Mutans Serotype C Gtfa Mutants In The Rat Model System, Raul G. Barletta, Suzanne M. Michalek, Roy Curtis Iii

School of Veterinary and Biomedical Sciences: Faculty Publications

The Streptococcus mutans serotype c gtfA gene encodes a 55-kilodalton protein which catalyzes the synthesis of a small glucan (1.5 kilodaltons) from sucrose (J. P. Robeson, R. G. Barletta, and R. Curtiss III, J. Bacteriol. 153:211-221, 1983). To investigate the role of the GtfA enzyme in virulence, we constructed S. mutans gtfA mutants from three cariogenic serotype c strains. A plasmid that carried an erythromycin resistance determinant and an internal fragment of the gtfA gene but that was unable to replicate in streptococci was used to transform S. mutans. The erythromycin-resistant transformants carried a partial duplication of the internal …

Cloning And Sequencing Of The Genes For Shiga Toxin From Shigella Dysenteriae Type 1, Nancy A. Strockbine, Matthew Jackson, Lawrence Sung, Randall Holmes, Alison D. O'Brien

United States Public Health Resources

The structural genes for Shiga toxin, designated stx A and stx B, were cloned from Shigella dysenteriae type 1 3818T, and a nucleotide sequence analysis was performed. Both stx A and stx B were present on a single transcriptional unit, with stx A preceding stx B. The molecular weight calculated for the processed A subunit was 32,225, while the molecular weight of the processed B subunit was 7,691. Comparison of the nucleotide sequences for Shiga toxin and Shiga-like toxin I (SLT-I) from Escherichia coli revealed that the genes for Shiga toxin and SLT-I were greater than 99% homologous; three nucleotide …

Improved Selective Medium For The Isolation Of Treponema Hyodysenteriae, Robert A. Kunkle, J. M. Kinyon

School of Veterinary and Biomedical Sciences: Faculty Publications

An agar medium with improved selection for Treponema hyodysenteriae was developed. Cultures of T. hyodysenteriae and T. innocens, feces from 11 clinically normal pigs, and colonic contents from 6 pigs with gross lesions consistent with swine dysentery were diluted in phosphate-buffered saline and plated on Trypticase soy agar (BBL Microbiology Systems, Cockeysville, Md.) with 5% citrated bovine blood (TSA), TSA with 400 ,ug of spectinomycin per ml (TSA-S400), TSA-S400 with 25 ,ug each of colistin and vancomycin per ml, and TSA with 5% pig feces extract and five antimicrobial agents (spiramycin, rifampin, vancomycin, colistin, and spectinomycin) (BJ). Viable numbers …

Purification Of Maturation-Promoting Factor, An Intracellular Regulator Of Early Mitotic Events, Manfred J. Lohka, Marianne K. Hayes, James L. Maller

School of Veterinary and Biomedical Sciences: Faculty Publications

Maturation-promoting factor causes germinal vesicle breakdown when injected into Xenopus oocytes and can induce metaphase in a cell-free system. The cell-free assay was used to monitor maturation-promoting factor during its purification from unfertilized Xenopus eggs. Ammonium sulfate precipitation and six chromatographic procedures resulted in a preparation purified >3000-fold that could induce germinal vesicle breakdown within 2 hr when injected into cycloheximide- treated oocytes. Proteins of 45 kDa and 32 kDa were correlated with fractions of highest activity in both assays. These fractions contained a protein kinase activity able to phosphorylate the endogenous 45-kDa protein, as well as histone Hi, phosphatase …

Sensitivity To Ingested Sulfites, Steve L. Taylor, Julie A. Nordlee

Department of Food Science and Technology: Faculty Publications

Sulfiting agents, including sodium and potassium bisulfite, sodium and potassium metabisulfite, sodium sulfite, and sulfur dioxide, have enjoyed widespread use as food and drug ingredients. The oral ingestion of these sulfiting agents is now known to trigger asthma in a small subset of the asthmatic population. The best evidence suggests that perhaps 150,000 to 200,000 individuals in the United States may be sulfite sensitive. Although the mechanism of sulfite-induced asthma remains unknown, several possibilities have been considered, including inhalation of sulfur dioxide (SO₂) while swallowing, an IgE-mediated reaction, and a deficiency of sulfite oxidase leading to impaired sulfite …

Catecholamine Training Effects From Exercise Programs: A Bridge To Exercise-Temperament Relationships, Richard A. Dienstbier, Robert L. Laguardia,, Marc Barnes, Gerald Tharp, Richard J. Schmidt

Department of Psychology: Faculty Publications

Three studies were conducted to test whether a catecholamine training effect results from a long-term aerobic exercise program. Study 1 showed significant increases in urinary adrenaline and noradrenaline following moderate mental stress/challenge for male aerobics subjects after a semester of training. Control groups of nonexercisers and continuously in-condition marathoners showed no comparable pre- to post semester catecholamine increases. Male and female Aerobics subjects were contrasted with nonexercisers across a semester in Study 2; the hypothesis was confirmed that postsemester increases in catecholamines occurred only following an episode of mental challenges/stress, and not following base-rate-rest conditions. Under conditions of more active …

Congruence Between Objective And Self-Report Data In A Sample Of Young Adolescents, Lisa J. Crockett, John E. Schulenberg, Anne C. Petersen

Department of Psychology: Faculty Publications

Methodology texts frequently emphasize the limitations of self-report measures. Empirical information on the validity of self-report data, however, tends to be limited to particular topics and populations. This paper examines the validity of self-report data in a sample of young adolescents for whom objective and self-report data were available on course grades, height, and weight. A comparison of the two kinds of data generally supported the validity of the self-reports, although there was some evidence of response effects. It was concluded that while young adolescents exhibit some systematic errors in reporting, self-reports can provide a useful substitute for some kinds …

Maternal And Nutritional Contributions To Infant Rats’Activational Responses To Ingestion, Brian H. Bornstein, Leslie M. Terry, Joseph A. Browde Jr.

Department of Psychology: Faculty Publications

Infant rats deprived of food, maternal care, and the opportunity to suckle display a dramatic behavioral activation and vigorously ingest when provided milk through oral cannulas. These experiments assessed which components of deprivation are important in producing these responses to milk. Nutritional deprivation alone, with or without the presence of an active maternal female, appears to be sufficient to produce ingestion. Behavioral activation, on the other hand, appears to require both nutritional deprivation and deprivation from a maternal female. The effect of maternal stimulation on later behavioral reactivity was not a function of the pups’ opportunity to suckle. However, active …

The Relationship Of Temperament To Tolerance Of Cold And Heat: Beyond “Cold Hands-Warm Heart”, Richard A. Dienstbier, Robert L. Laguardia, Noreen S. Wilcox

Department of Psychology: Faculty Publications

Thermal regulation and peripheral arousal in episodes of emotion are dependent upon similar autonomic and hormonal processes. Thermal-tolerance measure were developed and validated in order to indirectly assess adrenergic (cold tolerance) and cholinergic (heat tolerance) responsivity. We hypothesized that cold tolerance would correlate with reduced emotionality (largely fear and anxiety) and depression, and with increased stimulus seeking and dispositions toward activity; those hypotheses were confirmed. Tentatively advanced hypotheses that heat tolerance would correlate with reduced depression and emotional states were not confirmed, but heat tolerance was positively associated with activity and (weakly) with some of the same dimensions of temperament …

Shiga And Shiga-Like Toxins, Alison D. O'Brien, Randall K. Holmes

Uniformed Services University of the Health Sciences

Introduction ........................ 206

Shiga Toxin in Shigella Spp. ........................ 207

History ........................ 207

Purification and Structure ........................ 207

Mode of Action ........................ 208

Receptor binding and internalization ........................ 208

Inhibition of protein synthesis ........................ 209

Immunology and Immunochemistry ........................ 209

Genetics ........................ 210

Role of Toxin in Disease ........................ 210

Dysentery ........................ 210

Diarrhea ........................210

HUS ........................ 211

Shiga-like toxins in E. Coli ........................ 211

History and Nomenclature ........................ 211

Discovery that EPEC and EHEC make Shiga-like toxins ........................ 211

Relationship of Shiga-like toxins to Vero toxins ........................ 212

Purification and Structure ........................ 212

Mode of Action ........................ 212

Immunology …

1987 Beef Cattle Report, Irvin T. Omtvedt

Nebraska Beef Cattle Reports

Acknowledgments

Finishing

Determining Moisture of Grain Using Oven, Microwave, and Moisture Tester

Sarsaponin in Receiving Diets of Feedlot Cattle

Effect of Probiotics: Feedlot Receiving Diets

Effect of Roughage Source in Dry or High Moisture Whole Corn Finishing Diets

High Moisure Corn, Dry Grain Combinations for Finishing Cattle

Dry Corn, Wheat Combinations for Finishing Lambs, Cattle

Dry or Wet Corn Bran or Gluten Feed for Ruminants

Corn Gluten Feed: A Review

Methods of Estimating Heat Damage in Protein Sources

Altering Rumen Degradability of Soybean Meal Using Non-Enzymatic Browning

Slaughter Steer Basis, Nebraska Panhandle

Computer Prediction of Finishing Cattle Performance

Cow-Calf

Nutrition …

Pubertal Status And Psychosocial Development: Findings From The Early Adolescence Study, Lisa J. Crockett, Anne C. Petersen

Department of Psychology: Faculty Publications

Beginning in 1978, we have conducted a longitudinal study of early adolescent development. A major objective of this research has been to examine the influence of pubertal changes on other aspects of development during this period. With regard to pubertal effects we wished to address two major questions. First, what aspects of behavior seem to be affected by pubertal development? (In other words, are pubertal effects pervasive or relatively circumscribed, and if they are circumscribed, which aspects of behavior are involved?) Second, how does pubertal change exert an influence--does it operate directly on specific aspects of behavior and performance (for …

Late Fall Transmission Of Nematodirus Battus (Nematoda: Trichostrongyloidea) In Western Oregon, Lora G. Rickard, Eric P. Hoberg, Gary L. Zimmerman, Jannell K. Erno

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Studies on Nematodirus battusCrofton and Thomas, 1951, have shown a marked seasonality in the pattern of transmission. The life cycle normally involves only one parasitic generation per year. The infective third-stage larvae develop over the summer within eggs deposited on pasture. Eggs are sensitized by low temperatures during fall and winter; larval hatching follows in the presence of adequate moisture and proper osmotic conditions as the temperature rises above 10°C. This set of conditions, conducive to hatching, is regularly present only during spring; thus, hatching of eggs is concentrated into a few weeks during this time. Outbreaks of clinical …

Helminth Remains From Prehistoric Indian Coprolites On The Colorado Plateau, Karl J. Reinhard, Richard H. Hevly, Glenn A. Anderson

Karl Reinhard Publications

Examination of coprolites excavated from archaeological sites in the Americas demonstrates excellent preservation of helminth eggs and, in some cases, larvae. To gain an understanding of helminth parasitism in prehistory on the Colorado Plateau of Arizona, New Mexico, and Utah, 319 coprolites from 5 archaeological sites were analyzed. Helminth eggs and larvae were recovered after the coprolites were rehydrated, screened, and sedimented. At a sixth site, soils excavated from 5 rooms used as latrine areas were processed with palynological techniques. The results indicate that all but 1 of the prehistoric populations examined were infected with intestinal worms. The helminths implicated …

Prevalence Of Sensitivity To Sulfiting Agents In Asthmatic Patients, Robert K. Bush, Steve L. Taylor, Karen Holden, Julie A. Nordlee, William S. Busse

Department of Food Science and Technology: Faculty Publications

Ingestion of sulfiting agents can induce wheezing in some asthmatic patients. However, neither the prevalence of sulfite sensitivity nor the clinical characteristics of the affected asthmatic population are known. In a prospective single-blind screening study, 120 non-steroid-dependent and 83 steroid-dependent asthmatic patients underwent challenge with oral capsules of potassium metabisulfite. Five non-steroid-dependent and 16 steroid-dependent asthmatic patients experienced a greater than 20 percent reduction in their one-second forced expiratory volume within 30 minutes following the oral challenge. Twelve of these sulfite reactors were rechallenged with metabisulfite capsules in a double-blind protocol. Under these conditions, only three of seven steroid-dependent patients …

Natural And Experimental Infection With An Attaching And Effacing Strain Of Escherichia Coli In Calvest, Rodney A. Moxley, David H. Francis

School of Veterinary and Biomedical Sciences: Faculty Publications

Gnotobiotic calves were inoculated with an 05:K4:H^-, urease-positive strain of Escherichia coli isolated from a 2-day-old calf with diarrhea. The calves developed elevated temperatures and passed loose mucoid feces, with or without blood. The E. coli strain was negative for heat-stable and heat-labile enterotoxins but produced high levels of Shiga-like toxin. Bacteria attached diffusely to the epithelium of the large intestine and multifocally to the epithelium of the ileum. The duodenum and jejunum were not affected. At the sites of bacterial attachment, microvilli were effaced, enterocytes were degenerate, and necrosis and exfoliation had occurred. These results confirm a …

The Incidence Of Cryptosporidial Infections In Nebraska Dairy Calves, Douglas Lee Varner

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Within the last decade a new etiologic agent has been identified as a possible cause of diarrhea in humans and domestic animals—that being the protozoan parasite Cryptosporidium. The purpose of this study was to determine the incidence and distribution of Cryptosporidium in the state of Nebraska, to determine if Cryptosporidium occurs more frequently in association with other enteropathogens and to determine about the relation of scouring in the calves to infection with Cryptosporidium. A total of 620 fecal samples from 334 dairy calves from 71 herds in Nebraska were examined for cyptosporidial oocysts using the Sheather’s sugar flotation …

A Longitudinal Study Of Mesiodistal Crown Diameters In Human Primary And Permanent Dentitions, Randall S. Asher

Open Access Master's Theses (through 2010)

Dental practitioners treating children are often faced with the question of when to initiate orthodontic treatment. Preventive and interceptive orthodontics encourage treatment of young patients to guide developing malocclusions into functional and aesthetic harmony. Success in early orthodontic treatment depends on accurate diagnosis. Dimensional growth of the arches anterior to the first permanent molar is essentially complete by the age of eight, years of age. 24 Therefore, two important factors in dentition alignment may be the arch length anterior to the first permanent molars and the mesiodistal crown diameter (MDCD) of the permanent teeth in the arch.

The purpose of …

Two Toxin-Converting Phages From Escherichia Coli 0157:H7 Strain 933 Encode Antigenically Distinct Toxins With Similar Biologic Activities, Nancy A. Strockbine, Lilian R. M. Marques, John W. Newland, H. Williams Smith, Randall K. Holmes, Alison D. O'Brien

United States Public Health Resources

Escherichia coli 0157:H7 strain 933 contains two distinct toxin-converting phages (933J and 933W). The biologic activities and antigenic relationship between the toxins produced by 933J and 933W lysogens of E. coli K-12, as well as the homology of the genes that encode the two toxins, were examined in this study. The 933J and 933W toxins, like Shiga toxin produced by Shigella dysenteriae type 1, were cytotoxic for the same cell lines, caused paralysis and death in mice, and caused fluid accumulation in rabbit ileal segments. The cytotoxic activity of 933J toxin for HeLa cells was neutralized by anti-Shiga toxin, whereas …