Medicine and Health Sciences Commons^™

Supporting Narrative Retells For People With Aphasia Using Augmentative And Alternative Communication: Photographs Or Line Drawings? Text Or No Text?, Julie Griffith, Aimee R. Dietz, Kristy S.E. Weissling

Department of Special Education and Communication Disorders: Faculty Publications

Purpose: The purpose of this study was to examine how the interface design of an augmentative and alternative communication (AAC) device influences the communication behaviors of people with aphasia during a narrative retell task.

Method: A case-series design was used. Four narratives were created on an AAC device with combinations of personally relevant (PR) photographs, line drawings (LDs), and text for each participant. The narrative retells were analyzed to describe the expressive modality units (EMUs) used, trouble sources experienced, and whether trouble sources were repaired. The researchers also explored the participants’ perceived helpfulness of the interface features.

Results: The participants …

The Effects Of Heart Disease, Teresa Wallace

Nebraska College Preparatory Academy: Senior Capstone Projects

• Physical: The physical risks and complications for contracting cardiovascular disease and how to prevent it.

• Social: Being social with the patient is a good way to lead the patient to the right path of recovery whereas if the patient had some family problems and had no family to comfort them.

• Emotional: The family is affected as well as the patient and I will be discussing how they act and feel before the surgeries of their child/family members and after the surgery.

Handling Cattle Through High Heat Humidity Indexes, Rob Eirich

Nebraska Beef Quality Assurance Program

As cattlemen enter the summer months, they need to understand and deal with heat and humidity. Consider incorporating the following practices into management plans to help reduce additional stress.

Novel Gammaherpesvirus Functions 1 Encoded By Bovine Herpesvirus (Bovine Lymphotropic Virus), J. Jia, G. Delhon, E. R. Tulman, D. G. Diel, Fernando A. Osorio, X. Wen, G. F. Kutish, D. L. Rock

School of Veterinary and Biomedical Sciences: Faculty Publications

The genus Macavirus of the subfamily Gammaherpesvirinae includes viruses that infect lymphoid cells of domestic and wild ruminants and swine, causing asymptomatic latent infections in reservoir hosts. Here, we describe the genome of bovine herpesvirus 6 (BoHV-6), a

macavirus ubiquitous in healthy cattle populations. The BoHV-6 genome exhibited architecture conserved in macaviruses, including a repetitive H-DNA region and unique, 141 kilobase pair L

DNA region predicted to encode 77 genes. BoHV-6 encoded in variable genomic regions a novel complement of genes relative to other characterized macaviruses, likely contributing to distinctive aspects of BoHV-6 infection biology and host range. Most notably, …

Integrating Mitosis, Toxicity, And Transgene Expression In A Telecommunications Packet-Switched Network Model Of Lipoplex-Mediated Gene Delivery, Timothy M. Martin, Beata Wysocki, Jared P. Beyersdorf, Tadeusz A. Wysocki, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Gene delivery systems transport exogenous genetic information to cells or biological systems with the potential to directly alter endogenous gene expression and behavior with applications in functional genomics, tissue engineering, medical devices, and gene therapy. Nonviral systems offer advantages over viral systems because of their low immunogenicity, inexpensive synthesis, and easy modification but suffer from lower transfection levels. The representation of gene transfer using models offers perspective and interpretation of complex cellular mechanisms, including nonviral gene delivery where exact mechanisms are unknown. Here, we introduce a novel telecommunications model of the nonviral gene delivery process in which the delivery of …

Distillers Grains Supplementation In A Forage System With Spayed Heifers, Kari L. Gillespie, Terry J. Klopfenstein, James C. Macdonald Donald, Brandon L. Nuttelman, Cody J. Schneider, Jerry D. Volesky, Galen Erickson

Nebraska Beef Cattle Reports

Spayed heifers were developed into yearlings by grazing corn residue and bromegrass, followed by native range, and were finished on a common diet. Treatments were 2 lb or 5 lb of wet distillers grains with solubles (WDGS; DM basis) supplement on corn residue daily, and modified distillers grains with solubles (MDGS) fed at 0.6% BW daily or no MDGS during summer grazing. Feeding 5 lb increased winter ADG by 0.68 lb (year 1) or 0.40 lb (year 2) compared to 2 lb, and increased HCW after finishing. Summer supplementation increased summer ADG by 0.50 lb (year 1) or 0. 44 …

Feeding Elevated Levels Of Corn Silage And Mdgs In Finishing Diets, Dirk B. Burken, Brandon L. Nuttelman, Terry J. Klopfenstein Klopfenstein, Galen E. Erickson

Nebraska Beef Cattle Reports

A finishing experiment evaluatedsubstitution of corn silage and modified distillers grains with solubles (MDGS) in place of corn. The treatment arrangementwas a 2 X 2 + 1 factorial with 15 or 45% corn silage and 20 or 40% MDGS plus a control containing 5% cornstalks and 40% MDGS. There were interactions between corn silage and MDGS for carcass adjusted performance. As corn silage inclusion was increased in the diet, F:G increased when fed with 20% MDGS, however there was no difference when fed with 40% MDGS.

Transitioning Cattle From Ramp® To A Finishing Diet On Feedlot Performance And Feed Intake Variance, Cody J. Schneider, Brandon L. Nuttelman, Dirk B. Burken Burken, Terry J. Klopfenstein, Galen E. Erickson

Nebraska Beef Cattle Reports

Individually fed cattle were adapted to high grain diets with a traditional grain adaptation program or one of two RAMP^® adaptation programs. RAMP programs adapted cattle to a finishing diet gradually over 28 days in four steps or directly without an adaptation. Feed intake variance among d was greater for traditionally adapted cattle compared to either RAMP program, but DMI was not different during the adaptation period. Over the 138-day period, feedlot performance and carcass traits were not affected by adaptation treatment. Cattle fed RAMP for 10 days can be transitioned to a finishing ration containing 47.5% Sweet Bran …

Use Of A Pelleted Corn Residue Complete Feed In Receiving Diets, Sarah J. Peterson, Brandon L. Nuttelman, Dirk B. Burken Burken, James C. Macdonald, Matt K. Luebbe, Galen E. Erickson

Nebraska Beef Cattle Reports

The effects of feeding a complete pelleted feed to newly received steer calves (585 ± 4 lb; n = 1318 ) was compared to a control ration consisting of 32% (DM basis) wet or modified distillers grains, 32% alfalfa hay, 32% dry-rolled corn, and 4% supplement. The pelleted complete feed consisted of 35% corn residue and a blend of grain byproducts and minerals. Feeding the complete pelleted feed increased DMI but decreased ADG, thereby reducing feed efficiency. The pelleted feed numerically reduced morbidity. Feeding a complete pellet consisting of corn residue appears to be a viable option for receiving calves …

Comparison Of Long-Term Progestin-Based Synchronization Protocols On Fixed-Time Ai Pregnancy Rate In Beef Heifers, Rebecca A. Vraspir, Adam F. Summers, Doug O'Hare Hare, Larry D. Rowden, Rick N. Funston

Nebraska Beef Cattle Reports

Yearling Angus heifers at a commercial ranch in the Nebraska Sandhills were randomly assigned to one of two progestin-based fixed-time AI protocols (MGA or 14-day CIDR) to compare pregnancy rates. Heifers had similar fixed-time AI pregnancy rates between MGA and 14-day CIDR. A similar proportion of MGA and 14-day CIDR heifers displayed a second estrus; however, heifers previously synchronized with MGA tended to have a greater second AI pregnancy rate. Overall pregnancy rate was similar between MGA and 14-day CIDR treatments. The MGA system was the more cost effective synchronization protocol in this study.

Effect Of Feeding De-Oiled Wet Distillers Grains Plus Solubles On Beef Oxidation And Tenderness, Katherine I. Domenech, Kim A. Varnold, Michelle E. Semler, Michael D. Chao, Tommi F. Jones, Galen E. Erickson, Chris R. Calkins

Nebraska Beef Cattle Reports

Cattle fed a de-oiled wet distiller’s grains plus solubles (WDGS) diet were compared to cattle fed corn or traditional (full-fat) WDGS diets to determine effects on discoloration, oxidation, and tenderness of beef aged for seven and 21 days. At seven days of aging, dietary treatment had no effect on discoloration. At 21 days of aging, beef from cattle fed de-oiled WDGS had less oxidation than the corn control and several of the full-fat WDGS treatments. Although tenderness increased with aging and retail display, dietary treatment had no effect on tenderness. These findings suggest that these dietary treatments, followed by a …

Effect Of Feeding Different Types Of Byproducts And Concentrations Throughout A Beef Growing System On Ground Beef Color And Lipid Oxidation, Joe O. Buntyn, Brandy D. Cleveland, Amy L. Redfield Redfield, James C. Macdonald, Galen E. Erickson, Tommi F. Jones, Ty B. Schmidt, Gary A. Sullivan

Nebraska Beef Cattle Reports

The objective of this trial was to evaluate the effect of feeding different concentrations of wet distillers grains during winter backgrounding and either modified wet distillers grains or Sweet Bran^® during the finishing phase on ground beef color and lipid oxidation. After a 14 day aging period, ground beef patties were made and placed in a simulated retail display for seven days. There were no overall differences in lipid oxidation between treatments but was a treatment by day interaction for discoloration. Ground beef from heifers finished with modified wet distillers grains discolored at a greater extent when compared to …

Shelf Life Of Cooked Ground Beef Patties From Cattle Fed Wet Distillers Grains With Solubles, Nathan T. Dierks, Tommi F. Jones, Kimberly A. Varnold Varnold, Derek J. Schroeder, Amy L. Redfield, Gary A. Sullivan

Nebraska Beef Cattle Reports

Cattle were grazed without or with energy supplementation of wet distillers grains with solubles (WDGS) during backgrounding, and were finished on a corn-based diet with or without 35% WDGS. Ground beef patties were made from shoulder clods, cooked, and stored in a refrigerated or frozen state. Cattle supplemented with WDGS had greater lipid oxidation in cooked ground beef patties regardless of finishing diet or storage type.

Differences In Fecal Bacterial Community Composition Between Beef Steers Which Are High-Shedders And Low-Shedders Of Shiga Toxin-Producing Escherichia Coli (Stec), Nirosh D. Aluthge, Yoshitha A. Wanniarachchi, Brandon L. Nuttelman Nuttelman, Cody J. Schneider, Terry J. Klopfenstein, Galen E. Erickson, Samodha C. Fernando

Nebraska Beef Cattle Reports

The community composition of the fecal microbiota was compared between beef steers which were high-shedders and low-shedders of Shiga toxin-producing Escherichia coli. Based on Shannon and Chao 1 diversity indices, the high-shedders had a more diverse fecal bacterial community than the low-shedding steers. Members of the genus Prevotella were observed as being more abundant in the low-shedders compared to the high-shedders, while Succinivibrio were more abundant in the high-shedders. Isolation of specific bacteria which are significantly more abundant in low-shedders may pave the way to developing direct-fed microbials which are effective in reducing STEC shedding among high-shedding beef steers.

Optimal Marketing Date Of Steers Depends On Marketing Strategy, James C. Macdonald, Cody J. Schneider, Kelsey M. Rolfe Rolfe, Stephen D. Kachman, Terry J. Klopfenstein, Galen E. Erickson

Nebraska Beef Cattle Reports

Seven research trials conducted over five years at the University of Nebraska–Lincoln were summarized to determine how animal performance changes through the finishing period on a live and carcass weight basis. Live weight, carcass weight, carcass ADG, and carcass feed efficiency all changed quadratically throughout the feeding period; live ADG and live feed efficiency declined linearly. During times of negative profit margins, optimal profitability for steers marketed on a live-basis occurred by selling early, whereas optimal profitability was achieved by feeding steers marketed on a carcass-basis longer.

The Effects Of Diet And Cooler Aging On Specific Flavor Notes In Beef, Kimberly A. Varnold, Chris R. Calkins, Rhonda K. Miller Miller, Galen E. Erickson

Nebraska Beef Cattle Reports

Crossbred steers (n = 64) were grazed on warm- or cool-season grass-dominated pastures, without or with energy supplementation of wet distillers grains with solubles (WDGS), and were finished on a corn-based diet with or without 35% WDGS. Finishing on corn increased desirable flavor notes and decreasedundesirable flavor notes in both L. dorsi and B. femoris steaks. In addition, grazing on warm-season grasses increased the prevalence of undesirable flavors but was often dissipated by the addition WDGS supplementation. Longer aging periods tended to increase the prevalence of undesirable flavors, especially in B. femoris steaks. It is recommended producers provide WDGS supplementation, …

Adult Response To Olanzapine Or Clozapine Treatment Is Altered By Adolescent Antipsychotic Exposure: A Preclinical Test In The Phencyclidine Hyperlocomotion Model, Qing Shu, Gang Hu, Ming Li

Department of Psychology: Faculty Publications

This study examined how repeated olanzapine (OLZ) or clozapine (CLZ) treatment in adolescence alters sensitivity to the same drug in adulthood in the phencyclidine (PCP) hyperlocomotion model. Male adolescent Sprague-Dawley rats (postnatal day (P) 44–48) were first treated with OLZ (1.0 or 2.0 mg/kg, subcutaneously (sc)) or CLZ (10.0 or 20.0 mg/kg, sc) and tested in the PCP (3.2 mg/kg, sc)-induced hyperlocomotion model for five consecutive days. Then a challenge test with OLZ (0.5 mg/kg) or CLZ (5.0 mg/kg) was administered either during adolescence (~P 51) or after the rats matured into adults (~P 76 and 91). During adolescence, repeated …

Evaluating The Clinical And Physiological Effects Of Long Term Ultraviolet B Radiation On Guinea Pigs (Cavia Porcellus), Megan K. Watson, Adam W. Stern, Amber L. Labelle, Stephen Joslyn, Timonthy M. Fan, Katie Leister, Micah Kohles, Kemba Marshall, Mark A. Mitchell

School of Veterinary and Biomedical Sciences: Faculty Publications

Vitamin D is an important hormone in vertebrates. Most animals acquire this hormone through their diet, secondary to exposure to ultraviolet B (UVB) radiation, or a combination thereof. The objectives for this research were to evaluate the clinical and physiologic effects of artificial UVB light supplementation on guinea pigs (Cavia porcellus) and to evaluate the long-term safety of artificial UVB light supplementation over the course of six months. Twelve juvenile acromelanic Hartley guinea pigs were randomly assigned to one of two treatment groups: Group A was exposed to 12 hours of artificial UVB radiation daily and Group B …

Update Of The Who/Iuis Allergen Nomenclature Database Based On Analysis Of Allergen Sequences, C. Radauer, A. Nandy, F. Ferreira, Richard E. Goodman, J. N. Larsen, J. Lidholm, A. Pomés, M. Raulf-Heimsoth, P. Rozynek, W. R. Thomas, Heimo Breiteneder

Department of Food Science and Technology: Faculty Publications

The IUIS Allergen Nomenclature Sub-Committee, under the auspices of the World Health Organization and the International Union of Immunological Societies, maintains the systematic nomenclature of allergenic proteins and publishes a database of approved allergen names on its Web site, www.allergen.org. In this paper, we summarize updates of allergen names approved at the meetings of the committee in 2011 through 2013. These changes reflect recent progress in identification, cloning, and sequencing of allergens. The goals of this update were to increase consistency in the classification of allergens, isoallergens, and variants and in the incorporation of the evolutionary classification of proteins into …

Grain Sorghum Whole Kernel Oil Lowers Plasma And Liver Cholesterol In Male Hamsters With Minimal Wax Involvement, Bo Hyun Lee, Thomas M. Carr, Curtis Weller, Susan L. Cuppett, Ismail M. Dweikat, Vicki Schlegel

Department of Food Science and Technology: Faculty Publications

The lipid fraction of the grain sorghum whole kernel (GS-WK) (i.e., phytosterol rich oil or policosanol rich wax) responsible for lowering cholesterol in hamsters fed the crude lipid (wax + oil) was determined. As expected, hamsters fed an atherogenic diet for a four week period presented with higher plasma non-HDL plasma and liver esterified cholesterol than those on the low fat diet. However, the atherogenic diet containing 5% (w/w) oil significantly lowered non-HDL plasma and liver cholesterol. Although the 5% wax supplement did not affect either plasma or liver cholesterol, excreted neutral sterol and bile acid were slightly higher than …

A Prospective Study Of Periodontal Disease And Risk Of Gastric And Duodenal Ulcer In Male Health Professionals, Matthew R. Boylan, Hamed Khalili, Edward S. Huang, Dominique S. Michaud, Jacques Izard, Kaumudi J. Joshipura, Andrew T. Chan

Department of Food Science and Technology: Faculty Publications

Periodontal disease is characterized by microbial shifts within the oral cavity and chronic inflammation of the gingival tissue, and can lead to tooth loss. Several studies support an association between periodontal disease and risk of chronic systemic conditions, including vascular disease, chronic obstructive pulmonary disease, diabetes mellitus, and cancer, including gastric adenocarcinoma. These associations may be mediated by systemic inflammation, the immune response to periodontal infection, or direct invasion by pathogens. Previous studies of periodontal disease and risk of peptic ulcer have been limited by cross-sectional design, diagnoses that were not confirmed by medical record review, and a lack of …

Development And Evolution Of Risk Assessment For Food Allergens, Rene W. R. Crevel, Joseph Baumert, Athanasia Baka, Geert Houben, André C. Knulst, Astrid Kruizinga, Stefano Luccioli, Stephen L. Taylor, Charlotte B. Madsen

Department of Food Science and Technology: Faculty Publications

The need to assess the risk from food allergens derives directly from the need to manage effectively this food safety hazard. Work spanning the last two decades dispelled the initial thinking that food allergens were so unique that the risk they posed was not amenable to established risk assessment approaches and methodologies. Food allergens possess some unique characteristics, which make a simple safety assessment approach based on the establishment of absolute population thresholds inadequate. Dose distribution modelling of MEDs permitted the quantification of the risk of reaction at the population level and has been readily integrated with consumption and contamination …

Translating Reference Doses Into Allergen Management Practice: Challenges For Stakeholders, René W. R. Crevel, Joseph Baumert, Stefano Luccioli, Athanasia Baka, Sue Hattersley, Jonathan O.B. Hourihane, Stefan Ronsmans, Frans Timmermans, Rachel Ward, Yong-Joo Chung

Department of Food Science and Technology: Faculty Publications

Risk assessment describes the impact of a particular hazard as a function of dose and exposure. It forms the foundation of risk management and contributes to the overall decision-making process, but is not its endpoint. This paper outlines a risk analysis framework to underpin decision-making in the area of allergen cross-contact. Specifically, it identifies challenges relevant to each component of the risk analysis: risk assessment (data gaps and output interpretation); risk management (clear and realistic objectives); and risk communication (clear articulation of risk and benefit). Translation of the outputs from risk assessment models into risk management measures must be informed …

Bovine Herpesvirus 1 Productive Infection Stimulates Inflammosome Formation And Caspase 1 Activity, Jianlin Wang, Jeffrey Alexander, Matthew S. Wiebe, Clinton J. Jones

Nebraska Center for Virology: Faculty Publications

Bovine herpesvirus 1 (BoHV-1), a significant viral pathogen of cattle, causes inflammation in affected tissue during acute infection. Consequently, we tested whether productively infected bovine cells stimulate inflammasome formation. Expression of two components required for inflammasome formation, the DNA sensor IFI16 (gamma-interferon-inducible protein 16) and NLRP3 (NOD-like receptor family, pyrin domain containing 3), were induced in bovine kidney cells by eight hours after infection. IFI16 was detected in punctate granules localized to the cytoplasm and nucleus. During productive infection, more than ten times more cells were caspase 1 positive, which is activated following inflammasome formation. Two caspase 1 inhibitors had …

Cell- And Virus-Mediated Regulation Of The Barrier-To-Autointegration Factor’S Phosphorylation State Controls Its Dna Binding, Dimerization, Subcellular Localization, And Antipoxviral Activity, Augusta Jamin, April Wicklund, Matthew S. Wiebe

Nebraska Center for Virology: Faculty Publications

Barrier-to-autointegration factor (BAF) is a DNA binding protein with multiple cellular functions, including the ability to act as a potent defense against vaccinia virus infection. This antiviral function involves BAF’s ability to condense double-stranded DNA and subsequently prevent viral DNA replication. In recent years, it has become increasingly evident that dynamic phosphorylation involving the vaccinia virus B1 kinase and cellular enzymes is likely a key regulator of multiple BAF functions; however, the precise mechanisms are poorly understood. Here we analyzed how phosphorylation impacts BAF’s DNA binding, subcellular localization, dimerization, and antipoxviral activity through the characterization of BAF phosphomimetic and unphosphorylatable …

Characterization Of A Dual-Tropic Human Immunodeficiency Virus (Hiv-1) Strain Derived From The Prototypical X4 Isolate Hxbc2, Shi-Hua Xiang, Beatriz Pacheco, Dane Bowder, Wen Yuan, Joseph Sodroski

Nebraska Center for Virology: Faculty Publications

Human immunodeficiency virus type 1 (HIV-1) coreceptor usage and tropism can be modulated by the V3 loop sequence of the gp120 exterior envelope glycoprotein. For coreceptors, R5 viruses use CCR5, X4 viruses use CXCR4, and dual-tropic (R5X4) viruses use either CCR5 or CXCR4. To understand the requirements for dual tropism, we derived and analyzed a dual-tropic variant of an X4 virus. Changes in the V3 base, which allow gp120 to interact with the tyrosine-sulfated CCR5 N-terminus, and deletion of residues 310/311 in the V3 tip were necessary for efficient CCR5 binding and utilization. Thus, both sets of V3 changes allowed …

Live Siv Vaccine Correlate Of Protection: Immune Complex-Inhibitory Fc Receptor Interactions That Reduce Target Cell Availability, Anthony J. Smith, Stephen W. Wietgrefe, Liang Shang, Cavan S. Reilly, Peter J. Southern, Katherine E. Perkey, Lijie Duan, Heinz Kohler, Sybille Muller, James Robinson, John V. Carlis, Qingsheng Li, R. Paul Johnson, Ashley T. Haase

Nebraska Center for Virology: Faculty Publications

Principles to guide design of an effective vaccine against HIV are greatly needed, particularly to

protect women in the pandemic’s epicentre in Africa. We have been seeking these principles by

identifying correlates of the robust protection associated with SIV_mac239Δnef vaccination in the

SIV-rhesus macaque animal model of HIV-1 transmission to women. We have identified one

correlate of SIV_mac239Δnef protection against vaginal challenge as a resident mucosal system for

SIV-gp41 trimer antibody production and neonatal Fc receptor (FcRn)-mediated concentration of

these antibodies on the path of virus entry to inhibit establishment of infected founder populations

at the …

Review Of Epidemiology And Transmission Of Kaposi’S Sarcoma-Associated Herpesvirus, Veenu Minhas, Charles Wood

Nebraska Center for Virology: Faculty Publications

This review summarizes the current knowledge pertaining to Kaposi sarcoma-associated herpesvirus (KSHV) epidemiology and transmission. Since the identification of KSHV twenty years ago, it is now known to be associated with Kaposi’s sarcoma (KS), primary effusion lymphoma, and multicentric Castleman’s disease. Many studies have been conducted to understand its epidemiology and pathogenesis and their results clearly show that the worldwide distribution of KSHV is uneven. Some geographical areas, such as sub-Saharan Africa, the Mediterranean region and the Xinjiang region of China, are endemic areas, but Western Europe and United States have a low prevalence in the general population. This makes …

Seroprevalence Of Human Herpesvirus 8 And Hepatitis C Virus Among Drug Users In Shanghai, China, Tiejun Zhang, Ying Liu, Yuyan Zhang, Jun Wang, Veenu Minhas, Charles Wood, Na He

Nebraska Center for Virology: Faculty Publications

To elucidate and compare the seroprevalence of human herpesvirus 8 (HHV8) and hepatitis C virus (HCV) among Chinese drug users, a cross-sectional study of 441 participants, was conducted in Shanghai, China, from 2012 through 2013. Seventy-seven (17.5%) participants were found to be positive for HHV8 antibodies, while 271 (61.5%) participants were positive for HCV. No significant association between HHV8 seropositivity and drug use characteristics, sexual behaviors, HCV, or syphilis was observed. In contrast, a statistically significant association between HCV seropositivity and injected drug history (OR, 2.18, 95% CI 1.41–3.37) was detected, whereas no statistically significant association between HCV seropositivity and …

Vaccines Within Vaccines: The Use Of Adenovirus Types 4 And 7 As Influenza Vaccine Vectors, Eric A. Weaver

Nebraska Center for Virology: Faculty Publications

adenovirus Types 4 and 7 (ad4 and ad7) are associated with acute respiratory distress (aRD). In order to prevent wide- spread ad-associated aRD (ad-aRD) the United states military immunizes new recruits using a safe and effective lyophi- lized wildtype ad4 and ad7 delivered orally in an enteric-coated capsule. We cloned ad4 and ad7 and modified them to express either a GFP-Luciferase (GFPLuc) fusion gene or a centralized influenza H1 hemagglutinin (Ha1-con). BaLB/c mice were injected with GFPLuc expressing viruses intramuscularly (i.m.) and intranasally (i.n.). ad4 induced significantly higher luciferase expression levels as compared with ad7 by both routes. ad7 transduction …