Medicine and Health Sciences Commons^™

Alkaline Treated Wheat Straw Or Corn Stover Fed To Growing Calves, Adam L. Shreck, Brandon L. Nuttelman, Cody J. Schneider Schneider, Dirk B. Burken, Casey N. Macken, William A. Griffin, Galen E. Erickson, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

Four hundred sixty steer calves were fed CaO treated (5% of DM) or untreated wheat straw and corn stover with wet distillers grains plus solubles (WDGS) during a 69-day growing study. An interaction between crop residue and alkaline treatment was observed for ending BW and ADG. The relative response in ADG and ending BW due to alkaline treatment was greater for wheat straw compared to corn stover. Steers fed wheat straw diets had greater DMI and improved F:G compared to corn stover diets. Alkaline treatment increased DMI and improved F:G, although the F:G response was small. Growing calves on untreated …

Use Of Treated Corn Residues In Growing Diets, Sarah J. Peterson, Dirk B. Burken, James C. Macdonald Donald, Galen E. Erickson

Nebraska Beef Cattle Reports

A growing study compared the effectsof pelleting corn residue and treating with calcium oxide or calcium hydroxide. All diets contained 60% corn residue, 36% distillers products, and 4% supplement (DM basis). Steers consuming pelleted diets had increased DMI, greater ending BW, but poorer F:G compared to non-pelleted treatments. Diets containing the chemically treated corn stover had increased ADG and lower F:G compared to the non-treated diets. While both pelleting and chemical treatment with CaO increased DMI, and ADG, only the use of CaO improved feed efficiency.

Using Enspira™ To Improve Fiber Digestion, Jana L. Harding, Galen E. Erickson, James C. Macdonald Donald

Nebraska Beef Cattle Reports

Two experiments evaluated the effectof treating various feedstuffs with an enzyme(Enspira) on digestibility. Twelve feeds commonly fed to beef cattle were treated with four levels of the enzyme(0, 0.25, 0.50, or 0.75 lb of enzyme per ton of DM). Enzyme treatment increased in vitro DMD of high moisture corn (HMC), wet distillers grains plus solubles (WDGS), corn bran, and husks. There was a quadratic increasein gas production for corn leaves, as well as a linear increase in gas production for corn bran treated with increasing levels of Enspira. Treating feeds with the commercial enzyme Enspiraimproved in vitro digestibility of feeds …

Dryland Cover Crops As A Grazing Option For Beef Cattle, Alex H. Titlow, Jake A. Hansen, Matt K. Luebbe Luebbe, Terry J. Klopfenstein, Karla H. Jenkins

Nebraska Beef Cattle Reports

A two-year grazing study was conducted to evaluate forage quality and utilization of cover crops (CC) in dryland cropping systems compared to crested wheatgrass pastures (CWP). The CC mixture consisted of oats, peas and turnips planted in March with a no-till drill. Both CC and CWP were grazed during the month of June. Total tract dry matter digestibility and CP were greater for CC compared to CWP while NDF and ADF of CC were less. The CC was observed to have greater forage quality over both years and may produce similar amounts of forage as crested wheatgrass pastures allowing deferred …

Effect Of Irrigation Allocation On Perennial Grass Production And Quality, Gary W. Hergert, Karla H. Jenkins, James Margheim Margheim, Alex Pavlista, Rex Nielsen, Murali Darapuneni

Nebraska Beef Cattle Reports

Cool-season grass mixtures and warm-season grass mixtures were evaluated in 2010, 2011, and 2012 under varying irrigation levels to determine dry matter yield, CP, and TDN for beef cattle in the Nebraska Panhandle. As a generalization, when seasonal precipitation was average, irrigation levels over 10 inches resulted in no significant increase in either grass production or quality. Cool-season grasses produced more dry matter yield and maintained greater CP and TDN than warm-season grasses. In all three years, a mixture of wheatgrasses had greater forage yield than an orchardgrass monoculture or a mixture dominated by bromegrasses. In 2010 and 2011, treatments …

Effect Of Distillers Grains Supplementation On Calves Grazing Irrigated Or Non-Irrigated Corn Residue, Mandi Jones, James C. Macdonald, Galen Erickson Erickson, Terry J. Klopfenstein, Andrea K. Watson

Nebraska Beef Cattle Reports

Steer calves grazing irrigated or non-irrigated corn residue received supplementation of dried or modified distillers grains plus solubles (DGS) at 0.3, 0.7, or 1.1% of BW . Steers were individually supplemented daily through Calan gates. Daily gain improved quadratically with increasing supplementation (1.55 lb/day to 2.12 lb/day) and for calves grazing non-irrigated (2.02 lb/day) compared to irrigated (1.77 lb/day) corn residue. Feeding dry instead of modified DGS did not significantly impact ADG. Supplementing DGS to calves grazing corn residue increased gain during the winter period.

Nutrient And Tenderness Differences Of Beef From Heifers Due To Mutation Of The Myostatin Genehypothesized, Michelle E. Semler, Chris R. Calkins, Galen E. Erickson Erickson

Nebraska Beef Cattle Reports

Strip loins and eye of rounds were obtained from heifers genotyped with variations of the myostatin gene; 19 homozygous dominant (Angus), 20 heterozygous dominant (Angus x Piedmontese), and 20 homozygous recessive (Piedmontese). Steaks were aged for 14 days, cooked fresh (never frozen), and nutrient steaks were frozen three days postmortem. Meat from homozygous recessive heifers was equal in tenderness to homozygous dominant and heterozygous dominant heifers. Fat content of meat from homozygous recessive heifers decreased while moisture and protein increased compared to homozygous dominant and heterozygous dominant. Calorie content decreased with increasing copies of the recessive gene. Thus, meat from …

Effect Of Feeding De-Oiled Wet Distillers Grains Plus Solubles On Beef Fatty Acid Profiles, Katherine I. Domenech, Kim A. Varnold, Michelle E. Semler Semler, Michael D. Chao, Tommi F. Jones, Galen E. Erickson, Chris R. Calkins

Nebraska Beef Cattle Reports

A total of 336 steers were fed one of seven finishing diets: a corn-based control, 35%, 50%, or 65% inclusion of wet distillers grains plus solubles (WDGS), either traditional (full-fat) or de-oiled. At harvest, 15 low USDA Choice carcasses within each dietary treatment (n = 105) were selected to evaluate the effect of diet on the fatty acid profile of strip loin steaks aged seven days. Feeding WDGS increased the amount of polyunsaturated fatty acids (PUFA) in comparison to a corn based diet. Feeding de-oiled WDGS resulted in less PUFA’s than the full-fat WDGS diets. It seems that the removal …

Effects Of Feeding Distillers Grains In A Yearling Beef System On Meat Quality, Kelby M. Sudbeck, Kimberly A. Varnold, James C. Macdonald Donald, Chris R. Calkins, Galen E. Erickson

Nebraska Beef Cattle Reports

Distillers grains use while wintering on cornstalks during summer grazing and during the finishing period was evaluated to determine the effects of lifetime exposure to distillers grains on meat characteristics. Finishing diets with distillers grains increased discoloration in steaks following six days of retail display for steaks aged seven days, and after four days of retail display for steaks aged 21 days. Supplementation during summer grazing increased discoloration when cattle were not finished using distillers grains. There were no differences in oxidative rancidity among dietary treatments. Supplementing with distillers grains prior to finishing was not additive in impacting the color …

Effect Of Natural Antioxidant Concentration On Lipid Oxidation Of Ready-To-Eat Ground Beef Links From Cattle Fed Distillers Grains In Different Phases Of Production, Brandy D. Cleveland, Joe O. Buntyn, Amy L. Redfield Redfield, James C. Macdonald, Galen E. Erickson, Tommi F. Jones, Ty B. Schmidt, Gary A. Sullivan

Nebraska Beef Cattle Reports

Shelf life of cooked ground beef links with no, low, or high concentrations of a blend of natural plant extract antioxidant were compared to evaluate lipid oxidation over time. When no antioxidants were added, samples stored nine days or beyond were more oxidized than the samples with the addition of an antioxidant. No differences in lipid oxidation were observed between 0.13% and 0.20% antioxidant concentrations during similar days of refrigerated storage days. Therefore, the addition of natural antioxidants were effective at reducing oxidative rancidity, regardless the concentration of antioxidant.

Lipid Oxidation In Cooked Ground Beef Links From Cattle Fed Distillers Grains In Different Phases Of Production, Brandy D. Cleveland, Joe O. Buntyn, Amy L. Redfield Redfield, James C. Macdonald, Galen E. Erickson, Tommi F. Jones, Ty B. Schmidt, Gary A. Sullivan

Nebraska Beef Cattle Reports

Ground beef links from cattle fed high or low levels of distillers grains during backgrounding and Sweet Bran^® or modified wet distillers grains in finishing diets were compared to analyze oxidation over time. Ready-to-eat beef links from cattle fed 5 lb/head/day (DM basis) of wet distillers grains during backgrounding had greater oxidative rancidity with extended storage than those from cattle fed 2 lb/head/day (DM basis). Beef links from cattle finished with wet distillers grains oxidized more rapidly than those fed Sweet Bran. Therefore, cooked beef from cattle fed distillers grains during either phase of production (backgrounding or finishing) showed …

Replacement Of Grazed Forage And Animal Performance When Distillers Grains Are Fed In A Bunk Or On The Ground On Summer Range, Kari L. Gillespie, L. Aaron Stalker, Terry J. Klopfenstein Klopfenstein, Jerry D. Volesky, Jacki A. Musgrave

Nebraska Beef Cattle Reports

Forage savings and supplement loss caused by feeding on the ground were estimated when spayed yearling heiferswere fed modified distillers grains with solubles (MDGS) while grazing Sandhills summer range. Across two years, heifers fed 0.6% BW MDGS had 1.39 lb greater ADG and consumed approximately 17% less forage than non-supplemented heifers. Calculated loss of MDGS when fed on the ground was 5.6%. Supplementing MDGS decreased forage consumption approximately 17% and increased summer gains.

Effects Of Dietary Change On Viral-Bacterial Interactions In The Rumen Of Cattle, Christopher L. Anderson, Melissa L. Jolly, Adam L. Shreck Shreck, Galen E. Erickson, Terry J. Klopfenstein, Samodha C. Fernando

Nebraska Beef Cattle Reports

This ongoing study investigates the impact of diet and bacteriophage activity on the structuring of rumen microbial community composition and diversity. Fistulated cattle were acclimated to a given diet for 21 days before samples were collected and subsequently enriched for viral particles with tangential flow filtration. Taxonomic identification, abundance, and functional attributes were assigned to both bacterial and viral communities. Principle coordinate analysis of the bacterial communities revealed significant clustering based on diet. While diet drives the structuring of rumen bacterial communities, bacteriophages may maintain high, constant bacterial diversity.

Effect Of Micro-Aid® Supplementation On Nitrogen Losses From Manure, Andrea K. Watson, Galen E. Erickson, Terry J. Klopfenstein Klopfenstein, Mike J. Rincker

Nebraska Beef Cattle Reports

A 2x2 factorial designed experiment was used to study the effects of Micro-Aid and time on OM and N losses from manure, in a simulated feedlot pen setting. Manure was collected from cattle on a common diet, except for the addition of 1 g Micro-Aid /steer daily. Losses of OM were greater at 60 d than 30 d, and greater for control than Micro-Aid. Nitrogen losses at d 30 were similar between treatments but control pans had greater N losses at d 60. Feeding Micro-Aid to cattle may inhibit N volatilization from manure, enhancing the fertilizer value of manure.

The Effects Of Diet And Cooler Aging On Consumer Panel Scores For Beef, Kimberly A. Varnold, Chris R. Calkins, Rhonda K. Miller Miller, Galen E. Erickson

Nebraska Beef Cattle Reports

Crossbred steers (n = 64) grazed warm- or cool-season grasses, without or with energy supplementation of wet distillers grains with solubles (WDGS), and were finished on a corn-based diet with or without 35% WDGS. Finishing cattle on WDGS, especially after beingsupplemented with WDGS, caused declinesin flavor desirability scores of L. dorsi steaks. Conversely, grass type was more influential in B. femoris steaks with warm-season grasses generating lower consumer panel scores. Scores were not different from each other when supplementation was provided. It is recommended that producers provide WDGS supplementation and finish on an all-corn diet in order to create the …

The Effects Of Diet On The Biochemical Constituents Of Beef, Kimberly A. Varnold, Chris R. Calkins, Brandon L. Nuttelman Nuttelman, Lasika S. Senaratne-Lenagala, Justine J. Stevenson, Michelle E. Semler, Michael D. Chao, Tommi F. Jones, Galen E. Erickson

Nebraska Beef Cattle Reports

Crossbred steers (n = 64) were grazed on warm- or cool-season grasses, without or with energy supplementation of wet distillers grains with solubles (WDGS), and were finished on a corn-based diet with or without 35% WDGS. Grass-type was the major contributor in determining the biochemical composition of L. dorsi steaks, with warm-season grasses causing increased concentrations of moisture and zinc and decreased concentrations of magnesium. Aging 28 days instead of 7 days increased pH and caused an increased concentration of carbohydrates, and non-heme and heme iron in B. femoris steaks. Diet, especially grass type, during grazing, can alter the end …

Grass Type, Grazing Supplementation, And Finishing Diets Affect Beef Fatty Acids, Kimberly A. Varnold, Chris R. Calkins, Brandon L. Nuttelman Nuttelman, Lasika S. Senaratne-Lenagala, Tommi F. Jones, Timothy P. Carr, Galen E. Erickson

Nebraska Beef Cattle Reports

Crossbred steers (n = 64) were grazed on warm- or cool-season grasses, without or with energy supplementation of wet distillers grains with solubles (WDGS), and were finished on a corn-based diet with or without 35% WDGS. Grass type was the major contributor in determining the fatty acid profile, especially in the neutral lipid layer. Warm-season grasses decreased concentrations of most fatty acids compared to cool-season grasses. The provision of WDGS as an energy supplement while grazing dissipated any differences caused by grass type.

Vein Steak Differences In Strip Loins Of Heifers Due To Mutation Of The Myostatin Gene, Michelle E. Semler, Chris R. Calkins, Galen E. Erickson Erickson

Nebraska Beef Cattle Reports

Strip loins from heifers with variations of the myostatin gene; 19 homozygous dominant (Angus), 20 heterozygous dominant (Angus x Piedmontese), and 20 homozygous recessive (Piedmontese) were studied. Strip loins were cut into 1-inch thick steaks and total number of steaks and number of steaks with Gluteusmedius (often called vein steaks) were recorded. Strip loins from heterozygous dominant heifershad a greater number of non-vein steaks and decreased percentage of vein steaks compared to homozygous dominant and homozygous recessive samples. Differences in percentage of vein steaks were inconsistent and showed no meaningful pattern.

Effect Of Feeding Distillers Grains And Supplementing With Dietary Antioxidants On Ground Beef Color During Retail Display, Brandy D. Cleveland, Chad G. Bower, Amy L. Redfield Redfield, Gary A. Sullivan

Nebraska Beef Cattle Reports

Ground beef patties from cattle fed corn-based diets with no wet distillers grains (control), wet distillers grains (WDGS), WDGS + 1000 IU/head/day vitamin E, WDGS + 150 ppm/head/day, Ethoxyquin/TBHQ (Agrado Plus, Novus International, St. Louis, Mo.), or WDGS + 500 IU/head/day vitamin E + 150 ppm/head/day Ethoxyquin/TBHQ during the finishing phase were compared to analyze color stability during retail display. As display time increased, patties from all dietary treatments had greater discoloration and became darker, less red, and more yellow. Therefore, beef patties discolored during retail display, but the rate and degree of discoloration were unaffected by diet or antioxidant …

Effect Of Distillers Grains Plus Solubles Supplementation On Grazing Cattle Performance, Tyler L. Hasenauer, Terry J. Klopfenstein, James C. Macdonald Donald, Cody J. Schneider, Dirk B. Burken

Nebraska Beef Cattle Reports

Yearlings rotationally grazing smooth bromegrass were individually supplemented modified distillers grains plus solubles (MDGS) at .05, 0.4, 0.6, and 0.8% BW. Gain increased quadratically as MDGS level increased. Maximal ADG (2.95 lb/d) was predicted when supplementing level of 0.48% of BW. Economic analysis compared 0, 2, and 5 lb (DM) MDGS supplementation. When cattle ownership was retained through the feeding period, MDGS supplementation was profitable. Supplementation at 2 lb (DM) was more profitable than 5 lb (DM) when MDGS is above $265.63/ton (DM) or 85% the price of $7.50 /bu corn.

Global Analysis Of Chlorella Variabilis Nc64a Mrna Profiles During The Early Phase Of Paramecium Bursaria Chlorella Virus-1 Infection, Janet M. Rowe, Adrien Jeanniard, James R. Gurnon, Yuannan Xia, David D. Dunigan, James L. Van Etten, Guillaume Blanc

Nebraska Center for Virology: Faculty Publications

The PBCV-1/Chlorella variabilis NC64A system is a model for studies on interactions between viruses and algae. Here we present the first global analyses of algal host transcripts during the early stages of infection, prior to virus replication. During the course of the experiment stretching over 1 hour, about a third of the host genes displayed significant changes in normalized mRNA abundance that either increased or decreased compared to uninfected levels. The population of genes with significant transcriptional changes gradually increased until stabilizing at 40 minutes post infection. Functional categories including cytoplasmic ribosomal proteins, jasmonic acid biosynthesis and anaphase promoting …

Dynamic Attachment Of Chlorovirus Pbcv-1 To Chlorella Variabilis, Irina Agarkova, Brigitte Hertel, Xinzheng Zhang, Les Lane, Alexander Tchourbanov, David D. Dunigan, Gerhard Thiel, Michael G. Rossmann, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Chloroviruses infect their hosts by specifically binding to and degrading the cell wall of their algal hosts at the site of attachment, using an intrinsic digesting enzyme(s). Chlorovirus PBCV-1 stored as a lysate survived longer than virus alone, suggesting virus attachment to cellular debris may be reversible. Ghost cells (algal cells extracted with methanol) were used as a model to study reversibility of PBCV-1 attachment because ghost cells are as susceptible to attachment and wall digestion as are live cells. Reversibility of attachment to ghost cells was examined by releasing attached virions with a cell wall degrading enzyme extract. The …

Genetic Variation And Possible Mechanisms Driving The Evolution Of Worldwide Fig Mosaic Virus Isolates, Jeewan Jyot Walia, Anouk Willemsen, Eminur Elci, Kadriye Caglayan, Bryce W. Falk, Luis Rubio

Nebraska Center for Virology: Faculty Publications

Fig mosaic virus (FMV) is a multipartite negative-sense RNA virus infecting fig trees worldwide. FMV is transmitted by vegetative propagation and grafting of plant materials, and by the eriophyid mite Aceria ficus. In this work, the genetic variation and evolutionary mechanisms shaping FMV populations were characterized. Nucleotide sequences from four genomic regions (each within the genomic RNAs 1, 2, 3, and 4) from FMV isolates from different countries were determined and analyzed. FMV genetic variation was low, as is seen for many other plant viruses. Phylogenetic analysis showed some geographically distant FMV isolates which clustered together, suggesting long-distance migration. …

Risk Factors For Early Childhood Infection Of Human Herpesvirus-8 In Zambian Children: The Role Of Early Childhood Feeding Practices, Kay L. Crabtree, Janet M. Wojcicki, Veenu Minhas, David R. Smith, Chipepo Kankasa, Charles D. Mitchell, Charles Wood

Nebraska Center for Virology: Faculty Publications

Background—Human herpesvirus-8 (HHV-8) infection in early childhood is common throughout sub-Saharan Africa with prevalence increasing throughout childhood. Specific routes of transmission have not been clearly delineated, though HHV-8 is present in high concentrations in saliva.

Methods—To understand the horizontal transmission of HHV-8 within households to children we enrolled for cross-sectional analysis, 251 households including 254 children, age two and under, in Lusaka, Zambia. For all children, plasma was screened for HHV-8 and HIV-1 and health and behavioral questionnaires were completed. Multi-level logistic regression analysis was conducted to assess independent factors for HHV-8 infection in children.

Results—Risk factors for HHV-8 infection …

Turnip Crinkle Virus Coat Protein Inhibits The Basal Immune Response To Virus Invasion In Arabidopsis By Binding To The Nac Transcription Factor Tip, Teresa Donze, Feng Qu, Paul Twigg, T. Jack Morris

Nebraska Center for Virology: Faculty Publications

Turnip crinkle virus (TCV) has been shown to interact with a NAC transcription factor, TIP, of Arabidopsis thaliana, via its coat protein (CP). This interaction correlates with the resistance response manifested in TCV-resistant Arabidopsis ecotype Di-17. We report that failure of a mutated CP to interact with TIP triggered the corresponding TCV mutant (R6A) to cause more severe symptoms in the TCV-susceptible ecotype Col-0. We hypothesized that TCV regulates antiviral basal immunity through TIP-CP interaction. Consistent with this hypothesis, we found that the rate of accumulation of R6A was measurably slower than wild-type TCV over the course of an …

Effects Of Grazing On Nebraska Sandhills Meadow Forage Nutrient Content, Jared V. Judy, Jacki A. Musgrave, L. Aaron Stalker Stalker, Karla H. Jenkins, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

Nebraska Sandhills subirrigated meadow pastures were utilized to measure the effects of grazing on forage nutrient content in summer pastures. Pre-grazed pastures had greater protein and in vitro dry matter digestibility levelsand lower neutral detergent fiber levels compared with post-grazed pastures early in the grazing season. By late July, post-grazed vs. pre-grazed pastures did not differ in in vitro dry matter digestibility and neutral detergent fiber levels. Observed results indicate the greatest differences in nutrient content between post-grazed and pre-grazed pastures occur early in the grazing season.

Light Stimulates The Mouse Adrenal Through A Retinohypothalamic Pathway Independent Of An Effect On The Clock In The Suprachiasmatic Nucleus, Silke Kiessling, Patricia J. Sollars, Gary E. Pickard

School of Veterinary and Biomedical Sciences: Faculty Publications

The brain’s master circadian pacemaker resides within the hypothalamic suprachiasmatic nucleus (SCN). SCN clock neurons are entrained to the day/night cycle via the retinohypothalamic tract and the SCN provides temporal information to the central nervous system and to peripheral organs that function as secondary oscillators. The SCN clock-cell network is thought to be the hypothalamic link between the retina and descending autonomic circuits to peripheral organs such as the adrenal gland, thereby entraining those organs to the day/night cycle. However, there are at least three different routes or mechanisms by which retinal signals transmitted to the hypothalamus may be conveyed …

Dens Agenesis And Cervical Vertebral Malformation In A Labrador Retriever Puppy, Elisa Salas, Henry E. Cerny, Seth P. Harris

School of Veterinary and Biomedical Sciences: Faculty Publications

Cervical vertebral malformations can cause acute or progressive pain, paralysis, and potentially death. Herein we present a case report of dens agenesis; cervical vertebral malformations of C1, C2, C6, and C7; subluxation of C1 and C2 and C4 and C5; and associated degenerative myelopathy in a 5-month-old female Labrador Retriever puppy. We additionally review current knowledge regarding pathogenesis and treatment.

Viruses As Nanoparticles: Structure Versus Collective Dynamics, S. Sirotkin, A. Mermet, M. Bergoin, V. Ward, James L. Van Etten

James Van Etten Publications

In order to test the application of the “nanoparticle” concept to viruses in terms of low-frequency dynamics, large viruses (140–190 nm) were compared to similar-sized polymer colloids using ultra-small-angle x-ray scattering and very-low-frequency Raman or Brillouin scattering. While both viruses and polymer colloids show comparable highly defined morphologies, with comparable abilities of forming self-assembled structures, their respective abilities to confine detectable acoustic vibrations, as expected for such monodisperse systems, differed. Possible reasons for these different behaviors are discussed.

Viruses Infecting Marine Picoplancton Encode Functional Potassium Ion Channels, Fenja Siotto, Corinna Martin, Oliver Rauh, James L. Van Etten, Indra Schroeder, Anna Moroni, Gerhard Thiel

James Van Etten Publications

Phycodnaviruses are dsDNA viruses, which infect algae. Their large genomes encode many gene products, like small K+ channels, with homologs in prokaryotes and eukaryotes. Screening for K+ channels revealed their abundance in viruses from fresh-water habitats. Recent sequencing of viruses from marine algae or from salt water in Antarctica revealed sequences with the predicted characteristics of K+ channels but with some unexpected features. Two genes encode either 78 or 79 amino acid proteins, which are the smallest known K+ channels. Also of interest is an unusual sequence in the canonical α-helixes in K+ channels. Structural prediction algorithms indicate that the …