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Full-Text Articles in Medicine and Health Sciences

Exogenous Egf And Bdnf Treatment Enhances Differentiation And Proliferation Of Cultured Taste Cells, Mehmet Hakan Özdener, Nancy Ellen Rawson Jan 2012

Exogenous Egf And Bdnf Treatment Enhances Differentiation And Proliferation Of Cultured Taste Cells, Mehmet Hakan Özdener, Nancy Ellen Rawson

Turkish Journal of Medical Sciences

To determine the effects of epidermal growth factor (EGF) and brain-derived neurotrophic factor (BDNF) on taste cell generation by utilizing a long-term rat taste cell culture that exhibits molecular and functional characteristics of mature taste cells. The role of neurotrophic factors in taste cell development is not well understood. Materials and methods: A long-term rat taste cell culture exhibiting molecular and functional characteristics of mature taste cells was used. The effects of 3 different concentrations of EGF and BDNF on taste cell differentiation and proliferation were examined at 3 different time points. Results: We showed the presence of BDNF, EGF …


Ovariectomy Decreases Biomechanical Quality Of Skin Via Oxidative Stress In Rat, Ülkü Çömelekoğlu, Serap Yalin, Ebru Balli, Mehmet Berköz Jan 2012

Ovariectomy Decreases Biomechanical Quality Of Skin Via Oxidative Stress In Rat, Ülkü Çömelekoğlu, Serap Yalin, Ebru Balli, Mehmet Berköz

Turkish Journal of Medical Sciences

To investigate the effect of ovariectomy on the skin using biomechanical, biochemical, and histological techniques in the ovariectomized rat model. Ovariectomy causes significant changes in the physical characteristics of the skin. Materials and methods: Twenty female Wistar albino rats were divided into 2 groups, with each group consisting of 10 rats: the control group and the ovariectomized group. The ovariectomized group underwent bilateral ovariectomy via ventral incision and the control group underwent a sham operation. Skin biomechanics were measured 3 months following the ovariectomy with a tensile test using a biomaterial testing machine, and tensile strength, stress, strain, and toughness …


Thoracic Epidural Anesthesia With Bupivacaine Attenuates Systemic Inflammatory Response And Lung Impairment Associated With Cerulein-Induced Pancreatitis In Rats, Nurdan Bedi̇rli̇, Nalan Akyürek, Bahadir Kösem, Melek Yaman, Aslihan Çavunt Bayraktar, Ömer Kurti̇pek, Mustafa Kavutçu Jan 2012

Thoracic Epidural Anesthesia With Bupivacaine Attenuates Systemic Inflammatory Response And Lung Impairment Associated With Cerulein-Induced Pancreatitis In Rats, Nurdan Bedi̇rli̇, Nalan Akyürek, Bahadir Kösem, Melek Yaman, Aslihan Çavunt Bayraktar, Ömer Kurti̇pek, Mustafa Kavutçu

Turkish Journal of Medical Sciences

To evaluate the effects of thoracic epidural anesthesia (TEA) on acute pancreatitis (AP)-induced lung injury in a rat model. Materials and methods: A total of 24 rats were divided into 3 groups: the control group (n = 8; pancreatitis), the bupivacaine group (n = 8; pancreatitis + 20 µL/h 0.5% bupivacaine), and the saline group (n = 8, pancreatitis + 20 µL/h saline). Pancreatitis was induced by 4 intraperitoneal injections of 50 µg/kg cerulein. At 24 h, blood was collected for determination of amylase, blood gas, tumor necrosis factor-\alpha (TNF-\alpha), interleukin (IL)-6, and IL-1\beta; lung samples were collected for the …


The Effect Of Soya Bean Meal On Tibial Articular Cartilage Growth In Mice After Suckling Period: A Histomorphometric And Biochemical Study, Simin Fazelipour, Seyed Babak Kiaei, Amir Hossein Eghtesad, Zahra Tootian Jan 2012

The Effect Of Soya Bean Meal On Tibial Articular Cartilage Growth In Mice After Suckling Period: A Histomorphometric And Biochemical Study, Simin Fazelipour, Seyed Babak Kiaei, Amir Hossein Eghtesad, Zahra Tootian

Turkish Journal of Medical Sciences

To examine the effects of an oral supplement of soya bean meal on the histomorphometric alteration of tibia cartilage and the serum levels of calcium and alkaline phosphatase in 3-week-old female mice using a computer-assisted histomorphometric method. Materials and methods: Forty immature BALB/c female mice were selected and divided into 4 groups. They were fed for 3 months with 4 different regimens: a low-protein regimen without soya bean meal, 23% protein without soya bean meal, 20% of the total protein being provided by soya bean meal, and 40% of the total protein being provided by soya bean meal. After 3 …


Antinociceptive, Antiinflammatory, And Antioxidant Activities Of The Ethanol Extract Of Crataegus Orientalis Leaves*, Zeynep Bor, Rana Arslan, Nurcan Bektaş, Sevda Pirildar, Ali̇ Aslan Dönmez Jan 2012

Antinociceptive, Antiinflammatory, And Antioxidant Activities Of The Ethanol Extract Of Crataegus Orientalis Leaves*, Zeynep Bor, Rana Arslan, Nurcan Bektaş, Sevda Pirildar, Ali̇ Aslan Dönmez

Turkish Journal of Medical Sciences

To determine the in vitro antioxidant activity and to investigate the possible antinociceptive and antiinflammatory effects of the ethanol extract of Crataegus orientalis leaves. The medicinal use of extracts prepared from the leaves, flowers, and fruits of Crataegus (hawthorn) dates back to ancient times. It has been proposed that its antioxidant constituents account for these beneficial therapeutic effects. Materials and methods: To evaluate these effects, the hot-plate, tail-immersion, writhing, and carrageenan-induced edema tests were used. Moreover, the antioxidant activity of the extract was determined using the 2,2-diphenyl-1-picrylhydrazyl and \beta-carotene-linoleic acid assay systems. In the tests that were used to determine …


Violence Among High School Students In Malatya: A Prevalence Study, Osman Celbi̇ş, Leyla Karaoğlu, Mücahi̇t Eğri̇, Bora Özdemi̇r Jan 2012

Violence Among High School Students In Malatya: A Prevalence Study, Osman Celbi̇ş, Leyla Karaoğlu, Mücahi̇t Eğri̇, Bora Özdemi̇r

Turkish Journal of Medical Sciences

To determine the prevalence of violence-related behaviors on school property and to identify the predictors of youth violence among high school adolescents in Malatya. Materials and methods: Using a cluster sampling method, a cross-sectional descriptive study was carried out involving 1175 students. Backward logistic regression analysis was performed to evaluate the independent association existing between the potential risk factors and violence-related behaviors. Results: Of the students, 24.5% had been in a physical fight, 5.5% were injured and had to be treated by a doctor or nurse, and 4.3% had carried a weapon on school property during the 12 months preceding …


Seroprevalence Of Major Blood-Borne Infections Among Blood Donors In Kano, Nigeria, Emmanuel Nwankwo, Imoru Momodu, Ismaila Umar, Babashani Musa, Solomon Adeleke Jan 2012

Seroprevalence Of Major Blood-Borne Infections Among Blood Donors In Kano, Nigeria, Emmanuel Nwankwo, Imoru Momodu, Ismaila Umar, Babashani Musa, Solomon Adeleke

Turkish Journal of Medical Sciences

To examine the seroprevalence of common blood infections among blood donors, which remains a major concern in transfusion medicine in Nigeria. Blood transfusion is one of the known therapeutic interventions that cut across a number of clinical disciplines. Materials and methods: The seroprevalences of human immunodeficiency virus (HIV), hepatitis C virus (HCV), hepatitis B surface antigen (HBsAg), and syphilis among 280 blood donors at the Murtala Mohammed Specialist Hospital (MMSH) were determined. The seroprevalences of the pathogens were determined with regard to age, sex, donor category, and occurrence of association pathogens. Between June and December 2008, 276 males (98.6%) and …


A Comparative Investigation Of Anti-Müllerian Hormone (Amh) And Various Biochemical Parameters In Patients With Cryptorchidism, Oligospermia, Or Varicocele*, Turan Akdağ, Ali̇ Muhtar Ti̇fti̇k, Levent Sariyildiz Jan 2012

A Comparative Investigation Of Anti-Müllerian Hormone (Amh) And Various Biochemical Parameters In Patients With Cryptorchidism, Oligospermia, Or Varicocele*, Turan Akdağ, Ali̇ Muhtar Ti̇fti̇k, Levent Sariyildiz

Turkish Journal of Medical Sciences

To investigate the serum AMH and various biochemical parameter levels in patients with cryptorchidism, oligospermia, or varicocele. Materials and methods: This study included 100 participants, divided into 5 groups: cryptorchidism (n = 20), varicocele (n = 20), oligospermia (n = 20), and 2 control groups [control 1, 8-12 years old (n = 20) and control 2, 18-24 years old (n= 20)]. Using the blood samples drawn from both patient and control groups, AMH, testosterone, FSH, LH, and prolactin parameters were investigated. Results: AMH values measured were found to be 73.04 ± 44.5 ng/mL and 84.81 ± 59.1 ng/mL in 20 …


Prevalence Of Latent Prostate Cancer And Prostatic Intraepithelial Neoplasia In İstanbul, Turkey: An Autopsy Study, Nusret Akpolat, Yalçin Büyük, İbrahi̇m Uzun, İlhan Geçi̇t, Gülay Kurnaz Jan 2012

Prevalence Of Latent Prostate Cancer And Prostatic Intraepithelial Neoplasia In İstanbul, Turkey: An Autopsy Study, Nusret Akpolat, Yalçin Büyük, İbrahi̇m Uzun, İlhan Geçi̇t, Gülay Kurnaz

Turkish Journal of Medical Sciences

To investigate the frequency of latent prostatic carcinoma (PCa) and prostatic intraepithelial neoplasia (PIN) in the Turkish population. Materials and methods: PCa and PIN were evaluated in 116 male autopsy cases in which the cause of death was nontumoral. All patients were Turkish, living in İstanbul, and aged 40-79 years. Results: The prevalence of PCa was 19.8% and the decade rates (decades 5-8) were 9.5%, 12.5%, 18.8%, and 37%, respectively (P < 0.011). The rates of high-grade prostatic intraepithelial neoplasia (HGPIN) were 33.3%, 31.3%, 56.3%, and 25.9% for decades 5-8, respectively (P > 0.05). A total of 68.4% of PCa cases were HGPIN. There was a statistically significant correlation among HGPIN, PCa, and Gleason scores (P < 0.002). Conclusion: The prevalence of latent PCa in the Turkish population is very high (19.7%). In order to determine latent PCa cases, males over 40 years of age must be screened more strictly.


The Effects Of Axillary Block Using The Multiple Injection Method With Ropivacaine In Uremic And Nonuremic Patients*, Hülya Çevi̇k, Ahmet Mahli̇, Demet Coşkun Jan 2012

The Effects Of Axillary Block Using The Multiple Injection Method With Ropivacaine In Uremic And Nonuremic Patients*, Hülya Çevi̇k, Ahmet Mahli̇, Demet Coşkun

Turkish Journal of Medical Sciences

To compare the sensory and motor block quality in axillary brachial plexus block using the multiple injection method with ropivacaine in uremic and nonuremic patients. Materials and methods: Examined were 60 patients scheduled for orthopedic surgery of the distal upper extremity (nonuremic group: group N, n = 30) or creation of an arteriovenous fistula (uremic group: group U, n = 30) with an axillary brachial plexus block. The median, radial, ulnar, and musculocutaneous nerves were selectively localized by nerve stimulation. After obtaining an appropriate peripheral motor response, predetermined volumes of ropivacaine (0.5%), in accordance with a formula, were selectively injected …


Plasma Lipid Levels In Patients With Acute Bacterial Infections, Mohammad Nassaji, Raheb Ghorbani Jan 2012

Plasma Lipid Levels In Patients With Acute Bacterial Infections, Mohammad Nassaji, Raheb Ghorbani

Turkish Journal of Medical Sciences

To assess the impact of acute bacterial infections on plasma lipid levels and to determine the value of plasma lipid measurements in the diagnosis of acute bacterial infection. Materials and methods: A cross-sectional study was conducted involving 112 patients with acute bacterial infections admitted in hospital and 112 healthy individuals as controls. Levels of total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein, and triglycerides were measured in blood samples of all subjects. Mean levels of serum lipids were compared in both groups. Results: Both groups were matched based on age (P = 0.10), sex (P = 0.789), BMI (P = 0.515), …


Fractures In Young, Single-Humped Camels (Camelus Dromedarius), Ahmed F. Ahmed, Fahd Abdullah Al-Sobayil Jan 2012

Fractures In Young, Single-Humped Camels (Camelus Dromedarius), Ahmed F. Ahmed, Fahd Abdullah Al-Sobayil

Turkish Journal of Veterinary & Animal Sciences

The objectives were to study the frequencies and classification of fractures in young camels and to evaluate the clinical relevance of external fixation as a method of treatment. Cases of fractures (n = 75) in young camels (less than 2 years old) were studied. On admission, the cause, site, classification, and radiography of the fractures as well as the methods of treatment were investigated. Factors affecting fracture healing after treatment were investigated and analyzed. The frequencies of fracture were affected by breed (P = 0.001) and age (P = 0.01) but not sex. Trauma was the most common cause of …


Antioxidative And Antimicrobial Effects Of Garlic In Ground Camel Meat, Hamid Reza Gheisari, Vahid Reza Ranjbar Jan 2012

Antioxidative And Antimicrobial Effects Of Garlic In Ground Camel Meat, Hamid Reza Gheisari, Vahid Reza Ranjbar

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the antioxidative and antimicrobial effects of equivalent concentrations of garlic derivatives in ground camel meat during storage at 4 ± 1 °C. The addition of either garlic or butylated hydroxyanisole (BHA) (0.1 g/kg) significantly delayed lipid oxidation when compared with the control. The antioxidant activities of the various ingredients added followed the order of fresh garlic (FG), garlic powder (GP), BHA, and garlic oil (GO). After 14 days' storage, the aerobic plate count (APC) of both FG- and GP-formulated meat was significantly lower than that of either the control or BHA-formulated samples. …


A Determination Of Growth Hormone Receptor Gene Polymorphisms In East Anatolian Red Cattle, South Anatolian Red Cattle, And Turkish Grey Cattle, Iraz Akiş Akad, Ahmet Mengi̇, Kemal Özdem Öztabak Jan 2012

A Determination Of Growth Hormone Receptor Gene Polymorphisms In East Anatolian Red Cattle, South Anatolian Red Cattle, And Turkish Grey Cattle, Iraz Akiş Akad, Ahmet Mengi̇, Kemal Özdem Öztabak

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to determinate the genotype and allele distributions of growth hormone receptor gene polymorphisms in East Anatolian Red cattle (EAR), South Anatolian Red cattle (SAR), and Turkish Grey (TG) cattle. To this end, 50 EAR, 50 SAR, and 50 TG cattle were used in the study. DNA samples were isolated by using the standard ammonium acetate salt-out method. Target regions were amplified and digested by AluI, AccI, StuI, NsiI, and Fnu4HI restriction enzymes. The + allele frequency of growth hormone receptor (GHR)/AluI polymorphism related with milk traits in TG cattle and the - allele frequencies …


Optimum Timing For Operation In Bitches With Pyometra Related To Endotoxemia, Meli̇h Uçmak, Çağatay Tek, Mehmet Can Gündüz, Ahmet Sabuncu, Adem Şenünver, Arzu Funda Bağcigi̇l, Tülay Bakirel Jan 2012

Optimum Timing For Operation In Bitches With Pyometra Related To Endotoxemia, Meli̇h Uçmak, Çağatay Tek, Mehmet Can Gündüz, Ahmet Sabuncu, Adem Şenünver, Arzu Funda Bağcigi̇l, Tülay Bakirel

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the plasma levels of endotoxin in bitches with pyometra and to investigate the effects of preoperative supportive therapy on plasma endotoxin levels (LPS), the complete blood count, serum biochemistry parameters (alanine amino transferase (ALT), alkaline phosphatase (ALP), total protein, albumin, urea, and creatinine), clinical health status at the 12th, 24th, and 48th h following presentation, and to explore if they were correlated. Randomly allocated into 2 equivalent groups were 20 bitches with pyometra, aged from 3 to 16 years (mean 9.15 ± 3.65 years). After collection of the samples, bitches in ovariohysterectomy …


Relations Between Electrical Conductivity, Somatic Cell Count, California Mastitis Test And Some Quality Parameters In The Diagnosis Of Subclinical Mastitis In Dairy Cows, Güven Kaşikçi, Ömer Çeti̇n, Enver Bariş Bi̇ngöl, Mehmet Can Gündüz Jan 2012

Relations Between Electrical Conductivity, Somatic Cell Count, California Mastitis Test And Some Quality Parameters In The Diagnosis Of Subclinical Mastitis In Dairy Cows, Güven Kaşikçi, Ömer Çeti̇n, Enver Bariş Bi̇ngöl, Mehmet Can Gündüz

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the effectiveness of the electrical conductivity (EC) method on the diagnosis of subclinical mastitis in dairy cows comparing with somatic cell count (SCC) and California mastitis test (CMT), and also to investigate the effect of these values on the amount of total viable bacteria, density, freezing point, and mineral substances. A total of 386 milk samples collected from quarters of 188 cows at 10 different farms were used as materials. Of the samples, 258 (66.85%) were CMT (+), 85 (22.02%) were CMT (++), and 43 (11.13%) were CMT (+++). The mean EC and SCC …


Vaginal Prolapse Related To Ovarian Granulosa Cell Tumor In An Anatolian Shepherd., Deni̇z Nak, Ayli̇n Alasonyalilar Demi̇rer, Bi̇lgi̇ner Tuna, Yavuz Nak, Musa Özgür Özyi̇ği̇t Jan 2012

Vaginal Prolapse Related To Ovarian Granulosa Cell Tumor In An Anatolian Shepherd., Deni̇z Nak, Ayli̇n Alasonyalilar Demi̇rer, Bi̇lgi̇ner Tuna, Yavuz Nak, Musa Özgür Özyi̇ği̇t

Turkish Journal of Veterinary & Animal Sciences

A 4-year old, primiparous, female Anatolian Shepherd dog was presented for evaluation of type II vaginal prolapse with alopecia of the left perineal skin. Hematological analyses demonstrated leukocytosis and thrombocytopenia. Estradiol (E2) and progesterone (P4) levels were 156 pg/mL and 2.3 ng/mL, respectively. The vaginal smear was predominantly (at least 80%) made up of anuclear superficial cells. An abdominal ultrasonography revealed a heterogeneous multicystic mass with anechoic areas adjacent to the caudal pole of the left kidney. An ovariohysterectomy was performed. Ten days after the surgery, the prolapsed vagina was completely regressed. A histopathological examination of the ovarian mass demonstrated …


Interfragmental Fixation Of Rostral Mandibular Fracture With Cerclage Wire In A Thoroughbred English Horse, Mehmet Alper Çeti̇nkaya, Alper Demi̇rutku Jan 2012

Interfragmental Fixation Of Rostral Mandibular Fracture With Cerclage Wire In A Thoroughbred English Horse, Mehmet Alper Çeti̇nkaya, Alper Demi̇rutku

Turkish Journal of Veterinary & Animal Sciences

The patient in this study was a 20-year-old thoroughbred gelding. After clinical and radiographical assessments, a bilateral rostral mandibular body fracture was determined. Fracture stabilization was achieved via a bilateral interfragmentary cerclage wire application. The horse started to use its jaw within 24 h of surgery. Postoperative radiographs were taken until month 6, at different periods, and fracture healing was evaluated. Fixation materials were left in place. Fracture healing occurred with a rostral part malocclusion. The purpose of this study was to give clinical information about mandibular fractures and treatment of these fractures in a horse.


Congenital Lymphoma Of B-Cell Lineage In A Newborn Calf, Enver Beytut, Burhan Özba Jan 2012

Congenital Lymphoma Of B-Cell Lineage In A Newborn Calf, Enver Beytut, Burhan Özba

Turkish Journal of Veterinary & Animal Sciences

In this report, congenital lymphoma of B-cell lineage in a Brown Swiss calf is described. A large mass was seen on the head of the calf at birth. At necropsy, multiple masses were found on the skin and internal organs. A histopathological examination showed atypical lymphoid cells separated by connective tissue. None of the tumor cells showed a positive reaction to CD3, but the cells were immunopositive for CD79\alphacy. A CD45+ reaction confirmed a hematopoietic origin of the neoplasm. Tumor cells were also positive for lambda light chain IgG (\lambda IgG). Proliferating cell nuclear antigen (PCNA) immunostaining showed diffuse nuclear …


Equine Grass Sickness In Cyprus: A Case Report, Kypros Protopapas, Kyriakos Alexandros Spanoudes, Nikolaos Diakakis, Georgia Brellou Jan 2012

Equine Grass Sickness In Cyprus: A Case Report, Kypros Protopapas, Kyriakos Alexandros Spanoudes, Nikolaos Diakakis, Georgia Brellou

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.


Unusual Anal Atresia In A Lamb, Siavash Ahmadi Noorbakhsh Jan 2012

Unusual Anal Atresia In A Lamb, Siavash Ahmadi Noorbakhsh

Turkish Journal of Veterinary & Animal Sciences

The first report of an unusual anal atresia with growth of ectodermal organs (wool and skin) in the shape of the intestine inside the body is provided here. During reconstructive surgery, a rectum-like structure was noted, in which wool strands grew densely. A hypothetical mechanism by which this structure formed is presented on the basis of local alterations in fetal tissue proliferation and rate of development.


Optimum Temperature And Growth Performance Of Hatchery Reared Black Sea Flounder (Platichthys Flesus Luscus Pallas, 1814), İlhan Aydin, Orhan Ak, Ercan Küçük, Hamza Polat, Bi̇nnur Ceylan Jan 2012

Optimum Temperature And Growth Performance Of Hatchery Reared Black Sea Flounder (Platichthys Flesus Luscus Pallas, 1814), İlhan Aydin, Orhan Ak, Ercan Küçük, Hamza Polat, Bi̇nnur Ceylan

Turkish Journal of Veterinary & Animal Sciences

The present study focused on the effect of water temperature on growth and feed conversion ratio of juvenile Black Sea flounder (Platichthys flesus luscus; initial weight: 2.0 g ± 0.01 g). The experiment was carried out for 84 days with the following range of water temperatures: 13, 17, 21, and 25 °C. Growth, food consumption, and feed conversion ratio were the highest at 21 °C and the lowest at 13 °C. The growth rate of the fish increased with water temperatures up to 21 °C and it decreased significantly with a further increase in temperature. The conclusion of this study …


Seasonal And Group Effects On Dairy Cow Behavior In Large Yards, Akbar Nikkhah, Rasool Kowsar Jan 2012

Seasonal And Group Effects On Dairy Cow Behavior In Large Yards, Akbar Nikkhah, Rasool Kowsar

Turkish Journal of Veterinary & Animal Sciences

In mechanized modern dairy facilities with competitive environments, monitoring behavior provides opportunities to manipulate and optimize the nutritional, health, and social status of high-merit cows. The objective of the current study was to determine seasonal and cow group effects on the eating, ruminating, standing, and lying behaviors of dairy cows in large yards. Seasonal data on various behaviors of lactating cows in different production and lactation stages were collected continuously for 26 months, from December 2006 through February 2008. The herd had approximately 3000 dairy cattle housed in groups within specific yards. A total of 415 multiparous high-producing cows (MH), …


A Microscopic And Stereological Study Of The Renal Artery Transitional Zone Of The Adult Male Dog, Fatemah Ramezani Nowrozani Jan 2012

A Microscopic And Stereological Study Of The Renal Artery Transitional Zone Of The Adult Male Dog, Fatemah Ramezani Nowrozani

Turkish Journal of Veterinary & Animal Sciences

A transitional zone in the dog renal artery was documented and its parameters were recorded: length and volumes of the luminal wall, tunica intima, tunica media, and tunica adventitia-as well as these parameters in the non-transitional zone of artery. Twelve normal adult male dogs were studied. The specimens were processed in the routine manner for light and electron microscopy. After the determination of transitional zone length by light microscope, for the stereological study each renal artery was divided into transitional and non-transitional zones, and paraffin blocks were prepared. It was established that the transitional zone contains parallel and continuous elastic …


A Retrospective Study Of Borrelia Burgdorferi Antibodies In Dogs In Minnesota, Aneela Durrani, Sagar Goyal Jan 2012

A Retrospective Study Of Borrelia Burgdorferi Antibodies In Dogs In Minnesota, Aneela Durrani, Sagar Goyal

Turkish Journal of Veterinary & Animal Sciences

In order to determine the seroprevalence of Borrelia burgdorferi antibodies in suspected dogs in Minnesota, the present study examines the database of the Veterinary Diagnostic Laboratory at the University of Minnesota over a 10 year period (2001-June 2010). A total of 1081 serum samples from dogs suspected of having borreliosis were submitted by 112 private veterinary clinics. The samples were tested using an indirect fluorescent antibody (IFA) test. Samples with titers of =>1:320 were considered positive. The rate of seroprevalence of antibodies was 88%. The increase in the rate of yearly seroprevalence of Borrelia burgdorferi antibodies in dogs was …


Effects Of Cold Stress During Transportation On Hatchability And Chick Quality Of Broiler Breeder Eggs, Ahmad Salahi, Mozhdeh Moosanezhad Khabisi, Abbas Pakdel, Ali Baghbanzadeh Jan 2012

Effects Of Cold Stress During Transportation On Hatchability And Chick Quality Of Broiler Breeder Eggs, Ahmad Salahi, Mozhdeh Moosanezhad Khabisi, Abbas Pakdel, Ali Baghbanzadeh

Turkish Journal of Veterinary & Animal Sciences

In this experiment 72,000 broiler breeder eggs (Ross 308 strain) collected from 36-week-old flocks were subjected to cold stress during transportation. Eggs were allocated to 4 temperature groups (treatments): 1.2 °C, 1-2 °C, 2.5-3.9 °C, 4-6 °C, and a control group, 21-22 °C. Each treatment had 14,400 eggs, and data were analyzed in a completely randomized design. The results of this study showed that cold stress had a significant effect on percentage of egg weight loss (P < 0.001), and minimal egg weight loss occurred in the control group. The percentage of exploders and early hatched chicks and chick weight were higher in the below zero temperature treatment than the other groups (P < 0.01). Cold stress had a significant effect on chick length, hatchability, and the hatching of fertile eggs (P < 0.001). The effects of cold stress on chick yield and body weight uniformity were significant (P < 0.01). The effect of cold stress on hatchery byproduct efficiency was significant (P < 0.001), but did not affect fertility. Cold stress also had significant effects on early (1-8 days), middle (9-17 days), and late mortality (20-21 days); total embryo mortality; and exposed brain. Ectopic viscera was significant (P < 0.001), and most mortality was observed in below 4 °C treatments. Total percentages of malpositions and deformity (P < 0.001) and egg contamination at 1-9 days (first stage) and 10-21 days (second phase) were affected by cold stress (P < 0.001). Cold stress also had a significant impact on the number of cull chicks; percent of string navel, button navel, total string, and button; omphalitis; full body cavity; red hocks; dehydration; dirty chickens; and stubby down. Cold stress affects performance during incubation and overall chick quality.


Detection, Cloning, And Sequencing Of The Enterotoxin Gene Of Clostridium Perfringens Type C Isolated From Goat, Yuan Tang, Ming Wen, Bin Lian, Jianzhong Cheng, Kaigong Wang, Bijun Zhou Jan 2012

Detection, Cloning, And Sequencing Of The Enterotoxin Gene Of Clostridium Perfringens Type C Isolated From Goat, Yuan Tang, Ming Wen, Bin Lian, Jianzhong Cheng, Kaigong Wang, Bijun Zhou

Turkish Journal of Veterinary & Animal Sciences

Clostridium perfringens enterotoxin (CPE) is an important virulence factor involved in C. perfringens food poisoning and other gastrointestinal illnesses. In our experiment a Clostridium perfringens enterotoxin gene (cpe) positive type C strain was obtained by multiplex PCR from 34 C. perfringens isolates from goat in Guizhou Province, China. Then a pair of specific primers was designed and synthesized according to the published cpe gene, and the complete cpe gene was amplified from the genomic DNA of the cpe-positive C. perfringens isolate. The PCR product was cloned into pMD18-T vectors and transformed into competent cell DH5a. The recombinant plasmid was identified …


Prolonged Dystocia, Uterine Necrosis, And Ovariohysterectomy In A Chios Ewe, Christos N. Brozos, Lazaros Lazarides, Isidoros Karagiannis, Evangelos Kiossis, Georgios Tsousis, Vasilios Psychas, Nektarios Giadinis Jan 2012

Prolonged Dystocia, Uterine Necrosis, And Ovariohysterectomy In A Chios Ewe, Christos N. Brozos, Lazaros Lazarides, Isidoros Karagiannis, Evangelos Kiossis, Georgios Tsousis, Vasilios Psychas, Nektarios Giadinis

Turkish Journal of Veterinary & Animal Sciences

A 4-year-old Chios ewe was submitted to the Clinic of Farm Animals (School of Veterinary Medicine, Aristotle University of Thessaloniki). The animal had delivered 2 healthy lambs, 2 days earlier, and was inappetent, depressed, and reluctant to move, while it had tachycardia and tachypnea. The cervix was opened at a dilation of 1 finger in diameter and fetid fetal membranes expelled from the vagina. Serum biochemistry revealed renal and liver disease, while ultrasonography revealed uterine dilatation. Laparotomy revealed a partly autolysed embryo in the uterus and gangrenous metritis. Clostridium perfringens and Escherichia coli were isolated in bacterial cultures from the …


Dust And Endotoxin In Laying Hen Dwellings, Kristina Matkovic, Marija Vucemilo, Bara Vinkovic Jan 2012

Dust And Endotoxin In Laying Hen Dwellings, Kristina Matkovic, Marija Vucemilo, Bara Vinkovic

Turkish Journal of Veterinary & Animal Sciences

Only healthy laying hens are capable of producing an average of 280 eggs in 1 year of exploitation. Production of consume eggs takes place in intensive housing systems where the laying hens are still mainly kept in cages under controlled conditions. Technical infrastructure and hygiene quality do not guarantee a production atmosphere completely free from pollutants, including potential pathological risk factors. Laying hens and farm workers are exposed to large quantities of bioaerosols in henhouses. Bioaerosols represent a mixture of different biological particles which can, under certain circumstances, cause health and welfare problems in animals. The most important components are …


Immune System Dysfunction In Broiler Chickens Experimentally Inoculated With Fowl Adenovirus Serotype-4 Associated With Inclusion Body Hepatitis Hydropericardium Syndrome, Iftikhar Hussain, Muhammad Shahid Mahmood, Muhammad Imran Arshad, Masood Akhtar, Fazal Mahmood, Azhar Rafique Jan 2012

Immune System Dysfunction In Broiler Chickens Experimentally Inoculated With Fowl Adenovirus Serotype-4 Associated With Inclusion Body Hepatitis Hydropericardium Syndrome, Iftikhar Hussain, Muhammad Shahid Mahmood, Muhammad Imran Arshad, Masood Akhtar, Fazal Mahmood, Azhar Rafique

Turkish Journal of Veterinary & Animal Sciences

Inclusion body hepatitis-hydropericardium syndrome (IBH-HPS) in broilers is caused by an adenovirus, here referred to as inclusion body hepatitis-hydropericardium syndrome virus (IBH-HPSV). This study describes the immune status of broiler chickens experimentally inoculated with an isolate of fowl adenovirus-4 serotype-1, involved in IBH-HPS in various trials. When compared with the unchallenged controls, IBH-HPSV-inoculated broilers had significantly higher atrophy of the bursa of Fabricius (BF) by up to 2-fold, as well as of the thymus (up to 9-fold) and spleen (up to 1.5-fold) (P < 0.05), with up to 60% mortality. Upon challenge with sheep red blood cells (SRBCs), IBH-HPSV-inoculated birds had from 2- to 3-log lower anti-SRBC antibody titers than the control. Response to phytohemagglutinin-P (PHA-P) was significantly reduced in IBH-HPSV-inoculated birds (P < 0.05) as compared to the control. Results of the studies revealed that the immune status of broilers is compromised significantly during IBH-HPSV infection in terms of lymphoid organ integrity, humoral, and cell-mediated immune responses, respectively. These findings suggest that immune dysfunction could be a contributing factor in the increased mortality of birds affected by IBH-HPS, which renders the birds immunosuppressed for the rest of their lives.