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Articles 43741 - 43770 of 45148

Full-Text Articles in Medicine and Health Sciences

Quantitation Of Neuronal Loss Induced By Status Epilepticus And The Effects Of Nitric Oxide, Güldal Güleç, Behzat Noyan Jan 2000

Quantitation Of Neuronal Loss Induced By Status Epilepticus And The Effects Of Nitric Oxide, Güldal Güleç, Behzat Noyan

Turkish Journal of Medical Sciences

Neuronal loss in the hippocampal CA1 and CA3 fields due to lithium-pilocarpine- induced status epilepticus (SE) and the effects of a nitric oxide (NO) substrate, L-Arginine and a NO synthase inhibitor, N_w -nitro-L-Arginine methyl ester (L-NAME) on SE-induced brain damage were determined using the neuron counting procedure in rats. Rats were implanted with four chronic, epidural screw electrodes in order to obtain electrocortical recordings. SE was determined from the electrocortical recordings. After decapitation, the brains were removed, and the hippocampal sections were obtained and stained with hematoxylin and eosin. An analysis of variance (ANOVA) and Student's t- test were used …

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Biomimetic Preparation Of Ha Powders At 37°C In Urea-And Enzyme Urease-Containing Synthetic Body Fluids, Defne Bayraktar, A. Cüneyt Taş Jan 2000

Biomimetic Preparation Of Ha Powders At 37°C In Urea-And Enzyme Urease-Containing Synthetic Body Fluids, Defne Bayraktar, A. Cüneyt Taş

Turkish Journal of Medical Sciences

An important inorganic phase of synthetic bone applications, calcium hydroxyapatite (HA, Ca_10 (PO_ 4 ) 6_(OH_2 ), was prepared as a single-phase and sub-micron bioceramic powder. Carbonated HA powders were synthesized from calcium nitrate tetrahydrate and di-ammonium hydrogen phosphate salts dissolved in synthetic body fluid (SBF) solutions, containing urea (H_2 NCONH_2 ) and enzyme urease, under the biomimetic conditions of 37°C and pH 7.4, with a novel chemical precipitation technique. These powders were also found to contain trace amounts of Na and Mg ions in them, intentionally incorporated by using SBF solutions, instead of pure water, during their synthesis. The …

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The Use Of A Nonradioactive Digoxigenin Labeled Probe For Detection Of Apo(A) Gene Size, Yeşi̇m İlçöl, Bülent İlçöl, Yavuz Taga Jan 2000

The Use Of A Nonradioactive Digoxigenin Labeled Probe For Detection Of Apo(A) Gene Size, Yeşi̇m İlçöl, Bülent İlçöl, Yavuz Taga

Turkish Journal of Medical Sciences

Elevated lipoprotein(a) [Lp(a)] concentrations are associated with premature coronary heart disease and early myocardial infarction. In the general human population the sizes of apolipoprotein [apo(a)] isoforms are inversely associated with lipoprotein(a) levels. Large isoforms are associated with low Lp (a) and small isoforms with high Lp(a) in the plasma. The size of the polymophism is directly correlated with the number of kringle-4 domains in apo(a). It has been shown by quantitative Southern blotting, and more recently by pulsed-field gel electrophoresis, that polymophism is the result of differences in the number of tandem kringle-4 repeats in the apo(a) gene. In our …

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Establishment Of A Nonradioactive Molecular Diagnosis Of Fragile-X Syndrome, Zehra Oya Uyguner, Bernd Wollnik, Hülya Kayseri̇li̇, Turgut Tükel, Seher Başaran, Memnune Yüksel Apak Jan 2000

Establishment Of A Nonradioactive Molecular Diagnosis Of Fragile-X Syndrome, Zehra Oya Uyguner, Bernd Wollnik, Hülya Kayseri̇li̇, Turgut Tükel, Seher Başaran, Memnune Yüksel Apak

Turkish Journal of Medical Sciences

Fragile-X syndrome is a hereditary dynamic mutation disorder, predominantly caused by a large expansion of CGG trinucleotide repeats in the FMR 1 gene leading to methylation and down regulation of transcription of the gene. For the molecular diagnosis of the disease, the repeat locus in FMR 1 gene is primarily detected by Southern blotting with radioactively labeled probes. Unusually GC rich composition of the expanded region caused technical difficulties during PCR based testing and therefore is not performed by most diagnostic laboratories. We established a Southern blot method and a novel PCR protocol which enables the amplification of normal, premutated …

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Abnormalities Of The Midline Cavities Of The Brain: A Computerized Tomography Study, Zehra Akgün, Erdem Gümüşburun, Mustafa Akgün, H. Murat Göksel, Ferruh Yücel Jan 2000

Abnormalities Of The Midline Cavities Of The Brain: A Computerized Tomography Study, Zehra Akgün, Erdem Gümüşburun, Mustafa Akgün, H. Murat Göksel, Ferruh Yücel

Turkish Journal of Medical Sciences

This is a preliminary study carried out on the adult population of the Sivas region. The aim was to determine the incidence of Cavum Septum Pellucidum (CSP), Cavum Vergae (CV) and Cavum Velum Interpositi (CVI) and to compare the results with the data of previous studies and the relevant literature. A total of 442 computed tomography (CT) scans (302 males, 140 females), in axial plane, from the archive of the Department of Neurosurgery at Cumhuriyet University were examined to find out the brain midline cavities. Among the 442 CT scans, 69 (15.61%) had these brain midline cavities. Out of these …

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The Role Of Immunological Factors In Wound Infections (A Prospective Clinical Study), Nusret Akyürek, Mustafa Kerek, Osman Yüksel, Türkan Patiroğlu Jan 2000

The Role Of Immunological Factors In Wound Infections (A Prospective Clinical Study), Nusret Akyürek, Mustafa Kerek, Osman Yüksel, Türkan Patiroğlu

Turkish Journal of Medical Sciences

Wound infections are among the major problems complicating surgical procedures. The aim of this study was to investigate the relationship between the immunological status of the patient and wound infections. Wounds were classified as clean, clean-contaminated, contaminated and dirty. T-lymphocyte(CD3+), B-lymphocyte (CD19+) and total lymphocyte(CD45+) counts, and IgG, IgM, IgA levels were measured at the beginning of treatment, and on the fifth and tenth days. The levels of CD3+, CD19+, CD45+, IgGM, IgA were not different in the groups, while in the dirty group, IgG levels were lower than in the other groups, at the beginning of treatment (P0.05). There …

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Decreased Placental Adrenomedullin May Play Role In Preeclampsia, Remzi̇ Gökdeni̇z, Muhi̇tti̇n Yürekli̇, Alanur Menekşe, Nursel Bazoğlu Jan 2000

Decreased Placental Adrenomedullin May Play Role In Preeclampsia, Remzi̇ Gökdeni̇z, Muhi̇tti̇n Yürekli̇, Alanur Menekşe, Nursel Bazoğlu

Turkish Journal of Medical Sciences

Adrenomedullin (AdM) is a novel peptide that elicits a long-lasting vasorelaxant activity. It is expressed in several tissues, including adrenal medulla, heart, lung, kidneys, and cultured vascular smooth muscle cells. Also in large amounts, it is present in amniotic fluid and cord blood. The aim of this study was to assess placental AdM secretion in preeclampsia. Placental tissues were collected from seven preeclamptic patients and ten healthy gravidas. Tissue concentration of rat AdM was measured by using reverse-phase high performance liquid chromatography (Cecil 1100). Mann Whitney U test was used for statistical significance. Significance was set at p

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Prevalence Of Goitre And Its Relationship With Iodine Deficiency In School Children In Kocaeli, Turkey, Şükrü Hatun, Sidika Beykal, Yenal Dündar, Olcay Evli̇yaoğlu Jan 2000

Prevalence Of Goitre And Its Relationship With Iodine Deficiency In School Children In Kocaeli, Turkey, Şükrü Hatun, Sidika Beykal, Yenal Dündar, Olcay Evli̇yaoğlu

Turkish Journal of Medical Sciences

Prevalence of Goitre and Its Relationship with Iodine Deficiency in School Children in Kocaeli, Turkey 5564 primary school children living in Kocaeli, Turkey, were clinically assessed for thyroid gland enlargement. Goitre was graded according to the WHO 1994 classification. 792 children showed various grades of goitre, giving a prevalence of overall goitre of 14.23%. The median urinary iodine values were found to be greater than 10mg/dl in each group of 20 students, who were selected randomly from those in whom Grade I and Grade II goitre had been detected and from those in whom goitre had not been detected. These …

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Serum Antiotensin Converting Enzyme Activity In Streptozotocin-Induced Diabetic Rats, Mustafa Vakur Bor, Esra Seval Elmali, Ni̇lgün Altan Jan 2000

Serum Antiotensin Converting Enzyme Activity In Streptozotocin-Induced Diabetic Rats, Mustafa Vakur Bor, Esra Seval Elmali, Ni̇lgün Altan

Turkish Journal of Medical Sciences

No abstract provided.

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Effects Of Angiotensin Converting Enzyme Inhibitors In Healthy Rats And In Rats With Carbon Tetrachloride-Induced Toxic Hepatitis, Ercüment Ovali, Mustafa Çeti̇ner, Si̇ret Rati̇p, Fazil Aydin, Yavuz Tekeli̇oğlu, Sami̇ Karti, Asim Örem, Gökhan Harova, Levent Albayrak, Nazim Ağaoğlu Jan 2000

Effects Of Angiotensin Converting Enzyme Inhibitors In Healthy Rats And In Rats With Carbon Tetrachloride-Induced Toxic Hepatitis, Ercüment Ovali, Mustafa Çeti̇ner, Si̇ret Rati̇p, Fazil Aydin, Yavuz Tekeli̇oğlu, Sami̇ Karti, Asim Örem, Gökhan Harova, Levent Albayrak, Nazim Ağaoğlu

Turkish Journal of Medical Sciences

Sulphydryl-containing captopril (CPR) appears to act as a scavanger of oxygen derived free radicals. This is not present in other angiotensin-converting enzymes such as enalapril (EPR). The hepatotoxic effect of carbon tetrachloride (CCl4) may result from induction of reactive oxygen free radicals. The aim of this study was to analyse the effects of CPR and non- sulphydryl-containing enalapril (EPR) in healthy rats and in rats with CCL4-induced toxic hepatitis. The rats were divided into two major groups. The first group consisted of healthy rats, and the second group consisted of rats with CCl4- induced toxic hepatitis. Each major group was …

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Lineage-Restricted Function Of Nuclear Factor Kappab-Inducing Kinase (Nik) In Transducing Signals Via Cd40., Norman Garceau, Yoko Kosaka, Sally Masters, John Hambor, Reiko Shinkura, Tasuko Honjo, Randolph J. Noelle Jan 2000

Lineage-Restricted Function Of Nuclear Factor Kappab-Inducing Kinase (Nik) In Transducing Signals Via Cd40., Norman Garceau, Yoko Kosaka, Sally Masters, John Hambor, Reiko Shinkura, Tasuko Honjo, Randolph J. Noelle

Dartmouth Scholarship

CD40 signaling in B cells and dendritic cells (DCs) is critical for the development of humoral and cell-mediated immunity, respectively. Nuclear factor kappaB (NF-kappaB)-inducing kinase (NIK) has been implicated as a central transducing kinase in CD40-dependent activation. Here, we show that although NIK is essential for B cell activation, it is dispensable for activation of DCs. Such data provide compelling evidence that different intermediary kinases are used by different cellular lineages to trigger NF-kappaB activation via CD40.

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Effect Of Actinomycin D And Cycloheximide On Ischemic Preconditioning-Induced Delayed Cardioprotective Effect In Rats, Devinder Singh, Ajay Sharma, Manjeet Singh Jan 2000

Effect Of Actinomycin D And Cycloheximide On Ischemic Preconditioning-Induced Delayed Cardioprotective Effect In Rats, Devinder Singh, Ajay Sharma, Manjeet Singh

Pharmacy Faculty Articles and Research

The present study was designed to investigate the effect of actinomycin D, a transcription inhibitor, and cycloheximide, a translation inhibitor, on the delayed cardioprotective effect of ischemic preconditioning. Left thoracotomy was performed in anaesthetized rats at 4th/5th intercostal space and polypropylene suture (5-0) was employed to occlude left common coronary artery. Ischemic preconditioning was produced by four episodes of 5 min of coronary artery occlusion followed by 5 min of reperfusion and thoracic cavity was sutured. Left thoracotomy was performed again after 24 hr of ischemic preconditioning and left coronary artery was occluded for 30 min followed by reperfusion for …

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Molecular Investigations In The Role Of The Galk1 Gene In Galactokinase Deficiency, Michael L. Hunter Jan 2000

Molecular Investigations In The Role Of The Galk1 Gene In Galactokinase Deficiency, Michael L. Hunter

Theses : Honours

Galactokinase deficiency is an autosomal-recessive inborn error of galactose metabolism whose major clinical manifestation is the development of cataracts during the first months of life. This metabolic disorder is caused by defects in the first enzyme of the Leloir pathway, galactokinase, encoded by the gene GALK1 on chromosome 17q24. Despite the identification of a number of conserved domains in GALK1, understanding of the functional significance of these regions and the molecular basis of the disorder is limited. This is largely due to the rarity of the disease and the fact that the small number of GALK1 mutations identified to-date are …

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Ethical Issues In Dementia, Peter J. Whitehouse Jan 2000

Ethical Issues In Dementia, Peter J. Whitehouse

Faculty Scholarship

The growing number of individuals affected by dementia will intensify the ethical issues that emerge in clinical practice and research, issues early in disease relate to genetic testing, use of medications in mildly affected persons, and diagnostic disclosure. Research issues relate to appropriate informed consent processes, conflict of interests, and research design issues, such as the use of placebos and the use of biological tissues. In the later stages of disease concern about appropriate therapeutic goals and end-of-life care is appropriate.

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Isovolumic Regulation In Nervous Tissue: A Novel Mechanism Of Cell Volume Regulation, O. Quesada, R. Franco, K. Hernandez-Fonseca, K. Tuz Jan 2000

Isovolumic Regulation In Nervous Tissue: A Novel Mechanism Of Cell Volume Regulation, O. Quesada, R. Franco, K. Hernandez-Fonseca, K. Tuz

School of Veterinary and Biomedical Sciences: Faculty Publications

Cell volume regulation is a property present in most animal cell lineages that allows them to recover their original volume after events of swelling or shrinkage. Such events can be caused by changes in external osmolarity or to osmotic gradients generated during normal cell functioning.4,6 The mechanism of cell volume regulation involves transmembrane fluxes of osmotically active solutes in the necessary direction to counteract the net gain or loss of intracellular water.9 The process through which cells recover their normal volume after swelling is named Regulatory Volume Decrease (RVD). This consists of the efflux of inorganic osmolytes, such as K …

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A Cultivated Taste For Yeast., C Brenner Jan 2000

A Cultivated Taste For Yeast., C Brenner

Kimmel Cancer Center Faculty Papers

The availability of complete genomic sequences of Saccharomyces cerevisiae has catalyzed a cultural change in the practice of yeast biology, providing opportunities to develop high throughput techniques to define protein function, to define drug targets, and to discover and characterize drugs.

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Application Of Ai In Medicine, Tarmizi Amirul Hisyam Jan 2000

Application Of Ai In Medicine, Tarmizi Amirul Hisyam

Student Works (2000-2009)

From the beginning of human civilization, mankind have been trying to fins solution on how to make life easier. They create, modify and adapt, as they would please their need. From the smallest things in our life to the biggest part of all technologies, has improved our life tremendously. Computer technology nowadays has an important role in education, communication, medicine, and many more. In the field of medicine, we have seen many support systems had been developed and use to help human in their daily lives. Application of AI in medicine was proposed in this reason in mind, as a …

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Sistem Maklumat Perubatan Tradisional Melayu, Omar Rosfazila Jan 2000

Sistem Maklumat Perubatan Tradisional Melayu, Omar Rosfazila

Student Works (2000-2009)

Sistem Maklumat Perubatan Tradisional Melayu adalah merupakan satu sistem makumat berkomputer yang dibangunkan di halaman web. Ia bertujuan untuk membantu pengguna mencapai maklumat mengenai (ubatan tradisional serta tumbuhan berubat yang) diamalkan oleh orang Melayu. Sistem ini akan menceritakan serba sedikit tentang sejarah penggunaan perubatan tradisional. Pengguna sistem juga dijangka dapat mengemukan pertanyaan dan memberi sebarang komen yang berkaitan dengan sistem. Bantuan kepada pengguna sistem tentang cara menggunakan sistem turut dipaparkan dalam pembangunan system ini nanti. Sistem ini juga membolehkan pihak pentadbir membuat pengemaskinian maklumat mengikut kesesuaian semasa dan pembaharuan. Ia adalah sejajar dengan matlamat untuk memastikan penyimpanan maklwnat yang lebih …

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Sistem Rawatan Pesakit Mengikut Al-Quran Dan Sunnah (Srpas), Shafie Fazila Adini Jan 2000

Sistem Rawatan Pesakit Mengikut Al-Quran Dan Sunnah (Srpas), Shafie Fazila Adini

Student Works (2000-2009)

Di Malaysia, rawatan Islam, iaitu dengan menggunakan ayat-ayat al-Quran, hadis, contoh sunnah Rasulullah, doa-doa yang diamalkan oleh para anbiya' adalah merupakan satu alternatif yang masih lagi baru. Amalan ini sebenarnya satu kaedah yang jelas, teratur dan dapat dimanfaatkan oleh segenap lapisan masyarakat. Sistem rawatan pesakit mengikut al-quran dan sunnah (SRPAS ) merupakan satu sistem yang bertujuan untuk membantu serta menambahkan minat masyarakat kini tentang kepentingan rawatan Islam. Sistem ini juga sedikit sebanyak mampu mengatasi kekangan-kekangan dan masalah-masalah yang wujud dalam mengamalkan kaedah rawatan Islam. Selain daripada itu, sistem ini dapat menambah pengetahuan pengguna mengenai kelebihan nama-nama Allah dan fadhilat amalan …

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Evaluation Of Chitosan As A Potential Medical Iron (Iii) Ion Adsorbent, Ayer Burke, Elvan Yilmaz, Nesri̇n Hasirci Jan 2000

Evaluation Of Chitosan As A Potential Medical Iron (Iii) Ion Adsorbent, Ayer Burke, Elvan Yilmaz, Nesri̇n Hasirci

Turkish Journal of Medical Sciences

Chitosan, a derivative of the natural polysaccharide chitin, is known for its biocompatibility and metal-binding capacity. The aim of this study was to combine these two properties of chitosan and investigate its potential as a new iron (III) ion (ferric ion) adsorbing agent. The development of a safe and orally active iron chelating agent is especially important for the treatment of thalassemics. In this study, the physicochemical parameters affecting the ability of chitosan flakes to adsorb iron (III) ions were studied by complexometric titration. The results showed that the iron (III) adsorption capacity of chitosan increases with the amount of …

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Infective Panniculitis In A Child With Juvenile Rheumatoid Arthritis And Iga Deficiency, Ayça Törel Ergür, Meli̇h Akyol, Esi̇n Yildiz Jan 2000

Infective Panniculitis In A Child With Juvenile Rheumatoid Arthritis And Iga Deficiency, Ayça Törel Ergür, Meli̇h Akyol, Esi̇n Yildiz

Turkish Journal of Medical Sciences

No abstract provided.

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Biosynthesis Of Polyhydroxybutyrate And Its Copolymers And Their Use In Controlled Drug Release, Füsun Türesi̇n, Zeynep Gümüşyazici, Fatma N. Kök, İhsan Gürsel, N. Gürdal Alaeddi̇noğlu, Vasif Hasirci Jan 2000

Biosynthesis Of Polyhydroxybutyrate And Its Copolymers And Their Use In Controlled Drug Release, Füsun Türesi̇n, Zeynep Gümüşyazici, Fatma N. Kök, İhsan Gürsel, N. Gürdal Alaeddi̇noğlu, Vasif Hasirci

Turkish Journal of Medical Sciences

Our aim was to prepare antibiotic loaded rods with biotechnologically produced biodegradable polymers and use them in the treatment of osteomyelitis by providing a high local dose of antibiotic at the infected site. For that purpose, first the production of PHB and its copolymers (P(3HB- co-3HV) and P(3HB- co-4HB)) by Alcaligenes latus and Alcaligenes eutrophusin the shake-flask cultures and in a fed-batch fermenter and their purification and characterization were performed. The polymers were then used in the preparation of the sulbactam-cefoperazone loaded rods. To predict the in vivo behavior of the controlled release system, the in vitro release kinetics of …

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Elevated Serum \Beta-Hcg Levels In Severepreeclampsia, Remzi̇ Gökdeni̇z, Erdal Arigüloğlu, Nursel Bazoğlu, Özcan Balat Jan 2000

Elevated Serum \Beta-Hcg Levels In Severepreeclampsia, Remzi̇ Gökdeni̇z, Erdal Arigüloğlu, Nursel Bazoğlu, Özcan Balat

Turkish Journal of Medical Sciences

Most current hypotheses regarding the pathophysiologic mechanisms of pregnancy-induced hypertention point to early placental abnormalities. Therefore, we attempted to determine whether serum human chorionic gonadotropin (hCG) levels reflect a different trophoblastic secretory response of preeclampsia. Thirteen pregnant women with severe preeclampsia were matched with twenty-one normotensive pregnant women with singleton pregnancies in the third trimester. Serum b-hCG levels were found to be significantly higher in severe preeclampsia, compared with controls (p

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Mri In Joubert Syndrome, Nai̇l Bulakbaşi, Taner Üçöz, Cem Tayfun, Yüksel Pabuşçu, Alper Alemdaroğlu Jan 2000

Mri In Joubert Syndrome, Nai̇l Bulakbaşi, Taner Üçöz, Cem Tayfun, Yüksel Pabuşçu, Alper Alemdaroğlu

Turkish Journal of Medical Sciences

No abstract provided.

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The Effect Of Halothane On The Enzymatic Activity Of Mouse Liver And Erythrocyte Glucose-6- Phosphate Dehydrogenase, Ergül Belge Kurutaş, Güneş T. Yüregi̇r, İlhan Tuncer Jan 2000

The Effect Of Halothane On The Enzymatic Activity Of Mouse Liver And Erythrocyte Glucose-6- Phosphate Dehydrogenase, Ergül Belge Kurutaş, Güneş T. Yüregi̇r, İlhan Tuncer

Turkish Journal of Medical Sciences

The effects of repeated halothane anaesthesia on 6-10 week old male, Mus musculus albino mice weighing 29-32 g were studied. Halothane, a widely used anaesthetic, is known to be metabolised in the liver to toxic intermediates through cytochrome P4502E1 (CYP2E1). During the first week of repeated dosage, glucose-6- phosphate dehydrogenase (G6PDH) activity decreased in both erythrocytes and liver cells, and during this time, livers increased significantly in weight and suffered histopathological damage, the damage increasing with the dosage of halothane, in the range 0.25-1.0 ml twice daily. When repeated anaesthesia was continued for a second week, G6PDH activity increased significantly …

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Assessment Of Proliferative Activity In Soft Tissue Sarcomas Showing Pcna And Ki-67 Reactivity Immunohistochemically, Özlem Aydin, Canan Ersöz, Serdar Özbarlas, Gülşah Şeydaoğlu Jan 2000

Assessment Of Proliferative Activity In Soft Tissue Sarcomas Showing Pcna And Ki-67 Reactivity Immunohistochemically, Özlem Aydin, Canan Ersöz, Serdar Özbarlas, Gülşah Şeydaoğlu

Turkish Journal of Medical Sciences

The aim of this study was to determine PCNA (Proliferating Cell Nuclear Antigen) and Ki-67 expression of various soft tissue sarcomas (STS) immunohisto-chemically and to correlate with the histologic grade of these tumors. Retrospectively 185 cases of STS were re-examined. Of the 185 cases, 35 primary STS cases which were localized in the extremities and trunk wall were included in this study. Anti-PCNA and Anti-Ki-67 monoclonal antibodies were applied to the paraffin sections of tumor tissues immunohistochemically. A semiquantitative score was employed to assess the percentage of cells that were positively stained. The comparison of the positively stained tumor cell …

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Faculty Publications 1999, Lsu Health Sciences Center New Orleans Jan 2000

Faculty Publications 1999, Lsu Health Sciences Center New Orleans

Historical Faculty Publications

This Faculty bibliography contains 871 publications.

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The Global Carbon Metabolism Regulator Crc Is A Component Of A Signal Transduction Pathway Required For Biofilm Development By Pseudomonas Aeruginosa, George A. O'Toole, Karine A. Gibbs, Paul W. Hager, Paul V. Phibbs, Roberto Kolter Jan 2000

The Global Carbon Metabolism Regulator Crc Is A Component Of A Signal Transduction Pathway Required For Biofilm Development By Pseudomonas Aeruginosa, George A. O'Toole, Karine A. Gibbs, Paul W. Hager, Paul V. Phibbs, Roberto Kolter

Dartmouth Scholarship

The transition from a planktonic (free-swimming) existence to growth attached to a surface in a biofilm occurs in response to environmental factors, including the availability of nutrients. We show that the catabolite repression control (Crc) protein, which plays a role in the regulation of carbon metabolism, is necessary for biofilm formation in Pseudomonas aeruginosa. Using phase-contrast microscopy, we found that a crc mutant only makes a dispersed monolayer of cells on a plastic surface but does not develop the dense monolayer punctuated by microcolonies typical of the wild-type strain. This is a phenotype identical to that observed in mutants …

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Auto-Inhibition Of Ets-1 Is Counteracted By Dna Binding Cooperativity With Core-Binding Factor Α2, Tamara L. Goetz, Ting-Lei Gu, Nancy A. Speck, Barbara J. Graves Jan 2000

Auto-Inhibition Of Ets-1 Is Counteracted By Dna Binding Cooperativity With Core-Binding Factor Α2, Tamara L. Goetz, Ting-Lei Gu, Nancy A. Speck, Barbara J. Graves

Dartmouth Scholarship

Auto-inhibition is a common transcriptional control mechanism that is well characterized in the regulatory transcription factor Ets-1. Autoinhibition of Ets-1 DNA binding works through an inhibitory module that exists in two conformations. DNA binding requires a change in the inhibitory module from the packed to disrupted conformation. This structural switch provides a mechanism to tightly regulate Ets-1 DNA binding. We report that the Ets-1 partner protein core-binding factor α2 (CBFα2; also known as AML1 or PEBP2) stimulates Ets-1 DNA binding and counteracts auto-inhibition. Support for this conclusion came from three observations. First, the level of cooperative DNA binding (10-fold) was …

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Assessment Of Prothrombotic Tendency In Humans Using Functional And Genomic Determinants, Pádraig O'Sullivan Jan 2000

Assessment Of Prothrombotic Tendency In Humans Using Functional And Genomic Determinants, Pádraig O'Sullivan

Theses

Thrombophilia describes the familial or acquired disorders of the haemostatic mechanism that are likely to predispose to thrombosis. Venous thrombosis and its associated complications account for a significant number of hospital admissions and deaths annually having a significant health-economic impact on the heath-care industry globally. Mutations in genes that code for proteins involved directly (or indirectly) in blood coagulation have been associated with prothrombotic states. The majority of inherited genetic defects were attributed to polymorphisms in Antithrombin III, Protein C, and Protein S genes until 1994. Together these accounted for only 5-10% of individuals with thrombosis. Subsequently the Factor V …

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